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DE112015005476A5 - Verfahren und system zur detektion und unterscheidung zwischen mindestens zwei farbstoffen - Google Patents

Verfahren und system zur detektion und unterscheidung zwischen mindestens zwei farbstoffen Download PDF

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Publication number
DE112015005476A5
DE112015005476A5 DE112015005476.6T DE112015005476T DE112015005476A5 DE 112015005476 A5 DE112015005476 A5 DE 112015005476A5 DE 112015005476 T DE112015005476 T DE 112015005476T DE 112015005476 A5 DE112015005476 A5 DE 112015005476A5
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DE
Germany
Prior art keywords
distinction
dyes
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
DE112015005476.6T
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English (en)
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DE112015005476B4 (de
Inventor
Friedrich Schuler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hann-Schickard-Gesellschaft fuer Angewandte Forschung eV
Original Assignee
Hann-Schickard-Gesellschaft fuer Angewandte Forschung eV
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Application filed by Hann-Schickard-Gesellschaft fuer Angewandte Forschung eV filed Critical Hann-Schickard-Gesellschaft fuer Angewandte Forschung eV
Publication of DE112015005476A5 publication Critical patent/DE112015005476A5/de
Application granted granted Critical
Publication of DE112015005476B4 publication Critical patent/DE112015005476B4/de
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432Quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE112015005476.6T 2014-12-04 2015-12-04 Verfahren und system zur detektion und unterscheidung zwischen mindestens zwei farbstoffen Expired - Fee Related DE112015005476B4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102014117890.4 2014-12-04
DE102014117890 2014-12-04
PCT/EP2015/078646 WO2016087637A1 (de) 2014-12-04 2015-12-04 Verfahren und system zur detektion und unterscheidung zwischen mindestens zwei farbstoffen

Publications (2)

Publication Number Publication Date
DE112015005476A5 true DE112015005476A5 (de) 2017-08-24
DE112015005476B4 DE112015005476B4 (de) 2023-01-26

Family

ID=54782723

Family Applications (1)

Application Number Title Priority Date Filing Date
DE112015005476.6T Expired - Fee Related DE112015005476B4 (de) 2014-12-04 2015-12-04 Verfahren und system zur detektion und unterscheidung zwischen mindestens zwei farbstoffen

Country Status (2)

Country Link
DE (1) DE112015005476B4 (de)
WO (1) WO2016087637A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023203227A1 (de) 2022-04-22 2023-10-26 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Pcr-multiplexing durch zielsequenz-unabhängige-reportermoleküle mit unterscheidbaren signalstärken
EP4265735A1 (de) 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Pcr-multiplexing durch zielsequenz-unabhängige-reportermoleküle mit unterscheid-baren signalstäken

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH438489A (de) 1966-01-21 1967-06-30 Verein Foerd Festkoerperphysik Verfahren zum Steuern der Lumineszenzfarbe einer lumineszenzfähigen Substanz
DE2656632A1 (de) 1976-12-14 1978-06-15 Block Engineering Verfahren und vorrichtung zur untersuchung von fluoreszenzmaterial
DE4228366C2 (de) 1992-08-26 1995-05-24 Rainer Dr Uhl Fluoreszenz-Meßvorrichtung
US6252486B1 (en) 1997-06-13 2001-06-26 Philips Electronics North America Corp. Planar winding structure and low profile magnetic component having reduced size and improved thermal properties
EP0919604B1 (de) 1997-10-31 2004-01-02 The Pilot Ink Co., Ltd. Farbänderungsmaterial
US6447724B1 (en) 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
EP1321771A1 (de) 2001-12-21 2003-06-25 Evotec Analytical Systems GmbH Verfahren zur qualitativen und/oder quantitativen Bestimmung von Aggregaten
US8697029B2 (en) * 2002-04-18 2014-04-15 The Regents Of The University Of Michigan Modulated physical and chemical sensors
DE102008019756B4 (de) 2008-04-18 2011-03-17 Lavision Gmbh Verfahren zur simultanen Messung von Geschwindigkeits- und Zustandsparameterfeldern in einer Fluidströmung
DE102008025328B4 (de) 2008-05-27 2013-10-17 DRK-Blutspendedienst Baden-Württemberg-Hessen gGmbH Zusammensetzungen und Verfahren zur Amplifikation von HI-Viren mittels Multiplex-PCR in multiplen Genombereichen
DE102009026363B4 (de) 2009-08-11 2012-03-22 Deutsches Zentrum für Luft- und Raumfahrt e.V. Druckempfindliche Farbe
US20130178378A1 (en) 2011-06-09 2013-07-11 Andrew C. Hatch Multiplex digital pcr
DE102011083224A1 (de) 2011-09-22 2013-03-28 Infineon Technologies Ag Wärmeleitpaste, Leistungshalbleiteranordnung mit Wärmeleitpaste und Verfahren zum Aufbringen eines Wärmeleitmediums auf eine Wärmeableitfläche
DE102012203964B3 (de) 2012-03-14 2013-02-28 GNA Biosolultions GmbH Verfahren und Kit zur Detektion von Nukleinsäuren

Also Published As

Publication number Publication date
WO2016087637A1 (de) 2016-06-09
DE112015005476B4 (de) 2023-01-26

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