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CO6592070A2 - Remoción de transgenes en organismos geneticamente modificados - Google Patents

Remoción de transgenes en organismos geneticamente modificados

Info

Publication number
CO6592070A2
CO6592070A2 CO12128704A CO12128704A CO6592070A2 CO 6592070 A2 CO6592070 A2 CO 6592070A2 CO 12128704 A CO12128704 A CO 12128704A CO 12128704 A CO12128704 A CO 12128704A CO 6592070 A2 CO6592070 A2 CO 6592070A2
Authority
CO
Colombia
Prior art keywords
plant
nucleic acid
removal
zfns
specific
Prior art date
Application number
CO12128704A
Other languages
English (en)
Inventor
Sean M Russell
Joseph F Petolino
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44307617&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CO6592070(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of CO6592070A2 publication Critical patent/CO6592070A2/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8265Transgene containment, e.g. gene dispersal
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cultivation Of Plants (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para eliminar una región de ADN en una planta. En algunas modalidades, el método comprende transformar una planta con una molécula de ácido nucleico, en donde la molécula de ácido nucleico codifica una o más nucleasas del dedo de zinc (ZFNs) enlazada de manera operable a uno o más promotores específicos del tejido, por ejemplo, un promotor específico de polen. Los métodos incluyen la remoción de genes naturales en una planta. Por consiguiente, en algunas modalidades, son diseñados los ZFNs que reconocen secuencias que flanquean los genes naturales de la planta. En otra modalidad adicional, los ZNFs son expresados bajo el control de los promotores específicos de la etapa de desarrollo de modo que, por ejemplo, las secuencias de ácido nucleico son removidas específicamente en las plantas durante las etapas relativamente tardías de desarrollo. Se incluyen también moléculas de ácido nucleico útiles para llevar a cabo los métodos descritos y las plantas producidas mediante dichos métodos.
CO12128704A 2010-01-22 2012-07-31 Remoción de transgenes en organismos geneticamente modificados CO6592070A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29762810P 2010-01-22 2010-01-22

Publications (1)

Publication Number Publication Date
CO6592070A2 true CO6592070A2 (es) 2013-01-02

Family

ID=44307617

Family Applications (1)

Application Number Title Priority Date Filing Date
CO12128704A CO6592070A2 (es) 2010-01-22 2012-07-31 Remoción de transgenes en organismos geneticamente modificados

Country Status (20)

Country Link
US (3) US9695432B2 (es)
EP (1) EP2525649B1 (es)
JP (2) JP2013517774A (es)
KR (1) KR101951489B1 (es)
CN (1) CN102843904B (es)
AR (1) AR079968A1 (es)
AU (3) AU2011207387B2 (es)
BR (1) BR112012017896A2 (es)
CA (1) CA2787674C (es)
CL (1) CL2012002007A1 (es)
CO (1) CO6592070A2 (es)
EA (1) EA031356B1 (es)
GE (1) GEP201606544B (es)
IL (2) IL220870A (es)
MX (1) MX2012008485A (es)
NZ (1) NZ600546A (es)
PH (1) PH12012501467B1 (es)
UA (1) UA113493C2 (es)
WO (1) WO2011091311A2 (es)
ZA (1) ZA201204519B (es)

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Also Published As

Publication number Publication date
CN102843904B (zh) 2015-01-21
US20190112614A1 (en) 2019-04-18
AU2015202859A1 (en) 2015-06-18
CL2012002007A1 (es) 2012-10-19
GEP201606544B (en) 2016-09-26
NZ600546A (en) 2014-08-29
EP2525649B1 (en) 2020-01-08
ZA201204519B (en) 2013-09-25
PH12012501467A1 (en) 2012-10-22
US20110191877A1 (en) 2011-08-04
IL220870A (en) 2016-11-30
US9695432B2 (en) 2017-07-04
KR101951489B1 (ko) 2019-04-22
US20160257967A1 (en) 2016-09-08
AU2017265039A1 (en) 2017-12-07
UA113493C2 (xx) 2017-02-10
AU2011207387B2 (en) 2015-02-26
BR112012017896A2 (pt) 2015-09-01
MX2012008485A (es) 2012-08-17
IL248459B (en) 2018-06-28
WO2011091311A3 (en) 2011-11-17
KR20120116494A (ko) 2012-10-22
AU2011207387A1 (en) 2012-07-05
AR079968A1 (es) 2012-02-29
CA2787674C (en) 2022-09-06
WO2011091311A2 (en) 2011-07-28
EP2525649A2 (en) 2012-11-28
JP2013517774A (ja) 2013-05-20
AU2015202859B2 (en) 2017-10-19
JP6420270B2 (ja) 2018-11-07
IL220870A0 (en) 2012-09-24
JP2016144458A (ja) 2016-08-12
EA031356B1 (ru) 2018-12-28
CN102843904A (zh) 2012-12-26
PH12012501467B1 (en) 2018-07-04
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