CO6592070A2 - Remoción de transgenes en organismos geneticamente modificados - Google Patents
Remoción de transgenes en organismos geneticamente modificadosInfo
- Publication number
- CO6592070A2 CO6592070A2 CO12128704A CO12128704A CO6592070A2 CO 6592070 A2 CO6592070 A2 CO 6592070A2 CO 12128704 A CO12128704 A CO 12128704A CO 12128704 A CO12128704 A CO 12128704A CO 6592070 A2 CO6592070 A2 CO 6592070A2
- Authority
- CO
- Colombia
- Prior art keywords
- plant
- nucleic acid
- removal
- zfns
- specific
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8265—Transgene containment, e.g. gene dispersal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Cultivation Of Plants (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un método para eliminar una región de ADN en una planta. En algunas modalidades, el método comprende transformar una planta con una molécula de ácido nucleico, en donde la molécula de ácido nucleico codifica una o más nucleasas del dedo de zinc (ZFNs) enlazada de manera operable a uno o más promotores específicos del tejido, por ejemplo, un promotor específico de polen. Los métodos incluyen la remoción de genes naturales en una planta. Por consiguiente, en algunas modalidades, son diseñados los ZFNs que reconocen secuencias que flanquean los genes naturales de la planta. En otra modalidad adicional, los ZNFs son expresados bajo el control de los promotores específicos de la etapa de desarrollo de modo que, por ejemplo, las secuencias de ácido nucleico son removidas específicamente en las plantas durante las etapas relativamente tardías de desarrollo. Se incluyen también moléculas de ácido nucleico útiles para llevar a cabo los métodos descritos y las plantas producidas mediante dichos métodos.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29762810P | 2010-01-22 | 2010-01-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
CO6592070A2 true CO6592070A2 (es) | 2013-01-02 |
Family
ID=44307617
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CO12128704A CO6592070A2 (es) | 2010-01-22 | 2012-07-31 | Remoción de transgenes en organismos geneticamente modificados |
Country Status (20)
Country | Link |
---|---|
US (3) | US9695432B2 (es) |
EP (1) | EP2525649B1 (es) |
JP (2) | JP2013517774A (es) |
KR (1) | KR101951489B1 (es) |
CN (1) | CN102843904B (es) |
AR (1) | AR079968A1 (es) |
AU (3) | AU2011207387B2 (es) |
BR (1) | BR112012017896A2 (es) |
CA (1) | CA2787674C (es) |
CL (1) | CL2012002007A1 (es) |
CO (1) | CO6592070A2 (es) |
EA (1) | EA031356B1 (es) |
GE (1) | GEP201606544B (es) |
IL (2) | IL220870A (es) |
MX (1) | MX2012008485A (es) |
NZ (1) | NZ600546A (es) |
PH (1) | PH12012501467B1 (es) |
UA (1) | UA113493C2 (es) |
WO (1) | WO2011091311A2 (es) |
ZA (1) | ZA201204519B (es) |
Families Citing this family (46)
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US10260062B2 (en) * | 2010-01-22 | 2019-04-16 | Sangamo Therapeutics, Inc. | Targeted genomic alteration |
CA2831144A1 (en) | 2011-03-23 | 2012-09-27 | Pioneer Hi-Bred International, Inc. | Methods for producing a complex transgenic trait locus |
EP2734621B1 (en) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
CN103890181A (zh) * | 2011-08-22 | 2014-06-25 | 拜尔作物科学公司 | 修饰植物基因组的方法和手段 |
AP2014007861A0 (en) | 2012-01-23 | 2014-08-31 | Dow Agrosciences Llc | Herbicide tolerant cotton event PDAB4468.19.10.3 |
SG10201702445TA (en) | 2012-04-25 | 2017-04-27 | Regeneron Pharma | Nuclease-mediated targeting with large targeting vectors |
UA119135C2 (uk) * | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
JP6473419B2 (ja) * | 2012-12-13 | 2019-02-20 | ダウ アグロサイエンシィズ エルエルシー | 部位特異的ヌクレアーゼ活性のdna検出方法 |
DK3456831T3 (da) | 2013-04-16 | 2021-09-06 | Regeneron Pharma | Målrettet modifikation af rottegenom |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
EP4279496A3 (en) | 2013-09-04 | 2024-03-13 | Corteva Agriscience LLC | Rapid targeting analysis in crops for determining donor insertion |
US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
JP6560203B2 (ja) * | 2013-11-04 | 2019-08-14 | ダウ アグロサイエンシィズ エルエルシー | 遺伝子ターゲッティングのためのユニバーサルドナー系 |
MX2016007654A (es) | 2013-12-11 | 2017-08-15 | Regeneron Pharma | Metodos y composiciones para la modificacion dirigida de un genoma. |
US20150166985A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting von willebrand factor point mutations |
SI3152312T1 (sl) | 2014-06-06 | 2020-07-31 | Regeneron Pharmaceuticals, Inc. | Postopki in sestavki za spremembo ciljnega lokusa |
EP4079847A1 (en) | 2014-07-30 | 2022-10-26 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
EP3628739B1 (en) | 2014-09-12 | 2024-05-01 | Corteva Agriscience LLC | Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use |
EP3221457B1 (en) | 2014-11-21 | 2019-03-20 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modification using paired guide rnas |
EP3365357B1 (en) | 2015-10-23 | 2024-02-14 | President and Fellows of Harvard College | Evolved cas9 proteins for gene editing |
US10781455B2 (en) | 2016-06-20 | 2020-09-22 | Dow Agrosciences Llc | Methodologies and compositions for creating targeted recombination and breaking linkage between traits |
IL308426A (en) | 2016-08-03 | 2024-01-01 | Harvard College | Adenosine nuclear base editors and their uses |
US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
GB2573062A (en) | 2016-10-14 | 2019-10-23 | Harvard College | AAV delivery of nucleobase editors |
CA3043019A1 (en) * | 2016-11-21 | 2018-05-24 | Dow Agrosciences Llc | Site specific integration of a transgene using intra-genomic recombination via a non-homologous end joining repair pathway |
EP3555285A4 (en) * | 2016-12-14 | 2020-07-08 | Dow AgroSciences LLC | RECONSTRUCTION OF SITE SPECIFIC NUCLEASE BINDING SITES |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
EP3592777A1 (en) | 2017-03-10 | 2020-01-15 | President and Fellows of Harvard College | Cytosine to guanine base editor |
EP3601562A1 (en) | 2017-03-23 | 2020-02-05 | President and Fellows of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
EP3697906A1 (en) | 2017-10-16 | 2020-08-26 | The Broad Institute, Inc. | Uses of adenosine base editors |
EP3797160A1 (en) | 2018-05-23 | 2021-03-31 | The Broad Institute Inc. | Base editors and uses thereof |
EP3942042A1 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
EP3986118A4 (en) * | 2019-06-19 | 2023-10-04 | Pioneer Hi-Bred International, Inc. | COMPOSITIONS AND METHODS FOR IMPROVING CUSH BREAK TOLERANCE IN RAPS |
CN116096873A (zh) | 2020-05-08 | 2023-05-09 | 布罗德研究所股份有限公司 | 同时编辑靶标双链核苷酸序列的两条链的方法和组合物 |
US11242534B1 (en) | 2020-07-31 | 2022-02-08 | Inari Agriculture Technology, Inc. | INHT31 transgenic soybean |
US20240011043A1 (en) * | 2020-07-31 | 2024-01-11 | Inari Agriculture Technology, Inc. | Generation of plants with improved transgenic loci by genome editing |
BR112023001849A2 (pt) * | 2020-07-31 | 2023-02-23 | Inari Agriculture Tech Inc | Célula de planta de soja transgênica, parte de planta de soja transgênica, planta de soja transgênica, método para obter uma população a granel de sementes endogâmicas, método de obtenção de semente de soja híbrida, molécula de dna, produto de planta de soja transgênica processado, amostra biológica, molécula de ácido nucleico adaptada para detecção de dna genômico, método para detectar uma célula de planta de soja e método de excisão do lócus transgênico inht31 do genoma da célula de planta de soja |
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2011
- 2011-01-21 UA UAA201210056A patent/UA113493C2/uk unknown
- 2011-01-21 JP JP2012550172A patent/JP2013517774A/ja active Pending
- 2011-01-21 EP EP11735267.4A patent/EP2525649B1/en active Active
- 2011-01-21 EA EA201290678A patent/EA031356B1/ru not_active IP Right Cessation
- 2011-01-21 MX MX2012008485A patent/MX2012008485A/es not_active Application Discontinuation
- 2011-01-21 CA CA2787674A patent/CA2787674C/en active Active
- 2011-01-21 PH PH1/2012/501467A patent/PH12012501467B1/en unknown
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