CN216237044U

CN216237044U - Automatic change liquid-transfering nucleic acid and draw PCR all-in-one

Info

Publication number: CN216237044U
Application number: CN202122825400.4U
Authority: CN
Inventors: 刘永辉
Original assignee: Guangzhou Naique Medical Appliances Co ltd
Current assignee: Guangzhou Naique Medical Appliances Co ltd
Priority date: 2021-11-18
Filing date: 2021-11-18
Publication date: 2022-04-08
Anticipated expiration: 2031-11-18

Abstract

The utility model discloses an automatic pipetting nucleic acid extraction PCR all-in-one machine, which comprises a bottom plate and a manipulator assembly; a bottom plate: electric guide rails are arranged at the left end and the right end of the upper surface of the base plate in parallel, a sample rack is arranged at the front end of the upper surface of the base plate, a PCR instrument is arranged at the rear end of the upper surface of the base plate, a third linear motor is arranged in the middle of the upper surface of the base plate, and a 96-hole plate is placed on the bottom surface of a groove arranged on the upper surface of a rotor base of the third linear motor; a manipulator assembly: respectively with the active cell seat fixed connection of two electronic guide rails, the manipulator subassembly has two and parallel arrangement, and the manipulator subassembly front end lower surface of front side is equipped with extraction element, and extraction element sets up with 96 orifice plates and sample frame cooperation respectively, and the manipulator subassembly front end lower surface of rear side is equipped with the sucking disc, and the sucking disc sets up with 96 orifice plates and PCR appearance cooperation respectively, and this automation is moved liquid nucleic acid and is drawed PCR all-in-one, reduces the manual operation volume, has improved work efficiency.

Description

Automatic change liquid-transfering nucleic acid and draw PCR all-in-one

Technical Field

The utility model relates to the technical field of biological detection, in particular to an automatic pipetting nucleic acid extraction PCR all-in-one machine.

Background

The nucleic acid extracting device is an instrument for automatically completing the extraction of sample nucleic acid by using a matched nucleic acid extracting reagent, is widely applied to various fields of disease control centers, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like, the nucleic acid extracting method is divided into a centrifugal column method and a magnetic bead method, the PCR is called as a polymerase chain reaction, the PCR is a molecular biology technology for amplifying and amplifying specific DNA fragments, the PCR can be regarded as special DNA replication outside organisms, the PCR has the greatest characteristic that trace DNA can be greatly increased, so that the application of the PCR is very significant, and the PCR can be used for separating the DNA of one site from ancient organisms in fossil, remains of historical figures and hair, skin or blood left by murder in a murder case dozens of years ago, the PCR can be amplified and compared by using PCR, but the existing nucleic acid extraction and fluorescence PCR are carried out separately, PCR operation is carried out after nucleic acid cannot be extracted, and an extractor needs to be operated manually, so that the whole working efficiency is reduced.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects in the prior art, provides the automatic pipetting nucleic acid extraction PCR all-in-one machine, reduces the manual operation amount, improves the working efficiency, and can effectively solve the problems in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: an automatic liquid-transferring nucleic acid extraction PCR all-in-one machine comprises a bottom plate and a manipulator assembly;

a bottom plate: electric guide rails are arranged at the left end and the right end of the upper surface of the base plate in parallel, a sample rack is arranged at the front end of the upper surface of the base plate, a PCR instrument is arranged at the rear end of the upper surface of the base plate, a third linear motor is arranged in the middle of the upper surface of the base plate, and a 96-hole plate is placed on the bottom surface of a groove arranged on the upper surface of a rotor base of the third linear motor;

a manipulator assembly: the manipulator assembly is fixedly connected with the rotor bases of the two electric guide rails respectively, the manipulator assembly is provided with two parts and is arranged in parallel, the lower surface of the front end of the manipulator assembly on the front side is provided with an extraction device, the extraction device is matched with the 96 pore plate and the sample rack respectively, the lower surface of the front end of the manipulator assembly on the rear side is provided with a sucker, and the sucker is matched with the 96 pore plate and the PCR instrument respectively.

Wherein: still include the PLC controller, the PLC controller sets up in the upper surface front end of bottom plate, and external power source is connected to the input electricity of PLC controller, and electronic guide rail, PCR appearance and extraction element's input is connected to the output electricity respectively of PLC controller, with the sample of nucleic acid detection, mix extraction, tectorial membrane and the integration of a plurality of step systems of PCR detection, reduce the manual operation volume, improved work efficiency.

Further, the manipulator assembly comprises a first linear motor and a second linear motor, the two ends of the first linear motor are respectively fixedly connected with the rotor bases of the two electric guide rails, the front side face of the rotor base of the first linear motor is provided with the second linear motor, the front side face of the second linear motor on the front side is provided with an extraction device, the front side face of the second linear motor on the rear side is provided with a sucker, the input ends of the first linear motor and the second linear motor are electrically connected with the output end of the PLC, the two-axis manipulator is adopted for operation, and the working efficiency is improved.

Further, still include the laminating machine, the laminating machine sets up in the upper surface rear side of bottom plate, and the laminating machine sets up with the cooperation of 96 orifice plates, and PLC controller's output is connected to the input electricity of laminating machine, seals the test tube.

Furthermore, the PCR instrument is a fluorescent quantitative PCR instrument, and the whole PCR process is monitored in real time by utilizing fluorescent signal accumulation, so that the precision of nucleic acid detection is improved.

Further, the sucking disc is electronic vacuum chuck, and the output of PLC controller is connected to the input electricity of sucking disc, presss from both sides automatically to 96 orifice plates and gets and carry.

Compared with the prior art, the utility model has the beneficial effects that: this automatic move liquid nucleic acid extraction PCR all-in-one has following benefit:

placing a virus sampling tube in a jack of a sample rack, starting an electric guide rail through a PLC (programmable logic controller), driving a first linear motor at the front end of the electric guide rail to move forwards by a rotor seat at the front end of the electric guide rail, driving an extraction device to move downwards by a second linear motor at the front end, inserting the extraction device into the virus sampling tube to extract a blood sample, respectively controlling the second linear motor at the front end to move reversely by the PLC to draw out the extraction device upwards, driving the first linear motor at the front end to move backwards to the upper part of a 96-pore plate by the electric guide rail, inputting the blood sample in the extraction device into a test tube in the 96-pore plate, adding an extracting solution into the test tube in the 96-pore plate through the extraction device, controlling a third linear motor to swing back and forth by the PLC after all test tubes in the 96-pore plate are injected with the extracting solution, and oscillating the mixed solution of the sample and the extracting solution, vibrate the below that the third linear electric motor transferred the 96 orifice plate to the laminating machine after accomplishing, the laminating machine is to the test tube tectorial membrane of 96 orifice plate, the first linear electric motor of electronic guide rail rear side drives the rear side after the tectorial membrane is accomplished moves to the top of 96 orifice plate, PLC controller control sucking disc is fixed with 96 orifice plate centre gripping and upwards mentions, then carry to the inside of PCR appearance, start the PCR appearance and carry out the accounting detection analysis, this automatic move liquid nucleic acid and draw the PCR all-in-one, with the sample of nucleic acid detection, mix and draw, tectorial membrane and the integration of PCR detection multiple step system, reduce the manual operation volume, and the work efficiency is improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic cross-sectional plan view of the present invention.

In the figure: the device comprises a base plate 1, a PLC 2, an electric guide rail 3, a manipulator assembly 4, a first linear motor 41, a second linear motor 42, a third linear motor 5, a film laminating machine 6, a PCR 7 instrument, an 896 pore plate, a sample rack 9, an extraction device 10 and a sucker 11.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-2, the present embodiment provides a technical solution: an automatic liquid-transferring nucleic acid extraction PCR integrated machine comprises a bottom plate 1 and a manipulator assembly 4;

bottom plate 1: the left end and the right end of the upper surface of the base plate are parallelly provided with electric guide rails 3, the front end of the upper surface of the base plate 1 is provided with a sample rack 9, the rear end of the upper surface of the base plate 1 is provided with a PCR instrument 7, the PCR instrument 7 is a fluorescence quantitative PCR instrument, the whole PCR process is monitored in real time by utilizing fluorescence signal accumulation, the precision of nucleic acid detection is improved, the middle part of the upper surface of the base plate 1 is provided with a third linear motor 5, the bottom surface of a groove formed in the upper surface of a rotor base of the third linear motor 5 is provided with a 96-hole plate 8, the PLC controller 2 is used for controlling the third linear motor 5 to swing in a reciprocating manner to vibrate a mixed solution of a sample and an extracting solution, and the input end of the third linear motor 5 is electrically connected with the output end of the PLC controller 2;

wherein: still include PLC controller 2, PLC controller 2 sets up in the upper surface front end of bottom plate 1, and external power source is connected to PLC controller 2's input electricity, and electronic guide rail 3, PCR appearance 7 and extraction element 10's input are connected to PLC controller 2's output electricity respectively.

The manipulator assembly 4: the automatic sample clamping and conveying device is fixedly connected with the rotor seats of the two electric guide rails 3 respectively, the manipulator assembly 4 is provided with two parallel devices, the lower surface of the front end of the manipulator assembly 4 on the front side is provided with an extraction device 10, the extraction device 10 is matched with the 96 pore plate 8 and the sample rack 9 respectively, the lower surface of the front end of the manipulator assembly 4 on the rear side is provided with a sucker 11, the sucker 11 is an electric vacuum sucker, the input end of the sucker 11 is electrically connected with the output end of the PLC controller 2 to automatically clamp and convey the 96 pore plate 8, the sucker 11 is matched with the 96 pore plate 8 and the PCR instrument 7 respectively, the manipulator assembly 4 comprises a first linear motor 41 and a second linear motor 42, two ends of the first linear motor 41 are fixedly connected with the rotor seats of the two electric guide rails 3 respectively, the front side surface of the rotor seat of the first linear motor 41 is provided with the second linear motor 42, the front side surface of the rotor seat of the second linear motor 42 on the front side is provided with the extraction device 10, the sucking disc 11 is equipped with to the second linear electric motor 42 active cell seat leading flank of rear side, the equal electricity of the input of first linear electric motor 41 and second linear electric motor 42 connects the output of PLC controller 2, adopt the diaxon manipulator to operate, work efficiency has been improved, start electronic guide rail 3 through PLC controller 2, the active cell seat of electronic guide rail 3 front end drives the first linear electric motor 41 of front side and moves forward, the second linear electric motor 42 of front side drives extraction element 10 and moves down, extraction element 10 inserts the inside extraction blood sample of virus sampling pipe.

Wherein: still include laminating machine 6, laminating machine 6 sets up in the upper surface rear side of bottom plate 1, and laminating machine 6 sets up with the cooperation of 96 orifice plate 8, and PLC controller 2's output is connected to laminating machine 6's input electricity, and third linear electric motor 5 transfers 96 orifice plate 8 to the below of laminating machine 6, and laminating machine 6 seals the test tube to 96 orifice plate 8's test tube tectorial membrane.

The working principle of the automatic pipetting nucleic acid extraction PCR all-in-one machine provided by the utility model is as follows:

placing a virus sampling tube in a jack of a sample rack 9, starting an electric guide rail 3 through a PLC (programmable logic controller) 2, driving a first linear motor 41 at the front side to move forwards by a rotor base at the front end of the electric guide rail 3, driving an extraction device 10 to move downwards by a second linear motor 42 at the front side, inserting the extraction device 10 into the virus sampling tube to extract a blood sample, then respectively controlling the second linear motor 42 at the front side to move reversely by the PLC 2 to extract the extraction device 10 upwards, driving the first linear motor 41 at the front side to move backwards to the position above a 96-pore plate 8 by the electric guide rail 3, inputting the blood sample in the extraction device 10 into a test tube in the 96-pore plate 8, then adding an extracting solution into the test tube in the 96-pore plate 8 through the extraction device 10, and controlling a third linear motor 5 to swing back and forth by the PLC 2 after all test tubes in the 96-pore plate 8 are injected with the extracting solution, vibrate the mixed liquid of sample and extract, vibrate the below that the third linear electric motor 5 transferred 96 orifice plate 8 to laminating machine 6 after accomplishing, laminating machine 6 is to the test tube tectorial membrane of 96 orifice plate 8, the first linear electric motor 41 of the active cell seat drive rear side of electronic guide rail 3 rear side after the tectorial membrane is accomplished moves to the top of 96 orifice plate 8, PLC controller 2 control sucking disc 11 is fixed with the centre gripping of 96 orifice plate 8 and is upwards mentioned, then carry to the inside of PCR appearance 7, start PCR appearance 7 and carry out accounting detection analysis.

It should be noted that the electric guide rail 3, the first linear motor 41, the second linear motor 42, the PCR instrument 7, the extraction device 10, the suction cup 11, and the third linear motor 5 disclosed in the above embodiments can be freely configured according to practical application scenarios, the extraction device 10 can select a multi-channel liquid suction pump, the first linear motor 41 and the second linear motor 42 can select an MJ45 module, and the PLC controller 2 controls the electric guide rail 3, the first linear motor 41, the second linear motor 42, the PCR instrument 7, the extraction device 10, the suction cup 11, and the third linear motor 5 to operate by a method commonly used in the prior art.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. The utility model provides an automatic change liquid-transfering nucleic acid and draw PCR all-in-one which characterized in that: comprises a bottom plate (1) and a manipulator assembly (4);

base plate (1): electric guide rails (3) are arranged at the left end and the right end of the upper surface of the base plate in parallel, a sample rack (9) is arranged at the front end of the upper surface of the base plate (1), a PCR instrument (7) is arranged at the rear end of the upper surface of the base plate (1), a third linear motor (5) is arranged in the middle of the upper surface of the base plate (1), and a 96-hole plate (8) is placed on the bottom surface of a groove formed in the upper surface of a rotor seat of the third linear motor (5);

manipulator assembly (4): the manipulator assembly (4) is provided with two parallel arrangements, the lower surface of the front end of the manipulator assembly (4) on the front side is provided with an extraction device (10), the extraction device (10) is matched with a 96-hole plate (8) and a sample rack (9) respectively, the lower surface of the front end of the manipulator assembly (4) on the rear side is provided with a sucker (11), and the sucker (11) is matched with the 96-hole plate (8) and a PCR instrument (7) respectively.

Wherein: still include PLC controller (2), PLC controller (2) set up in the upper surface front end of bottom plate (1), and external power source is connected to the input electricity of PLC controller (2), and the input of electronic guide rail (3), PCR appearance (7) and extraction element (10) is connected to the output electricity of PLC controller (2) respectively.

2. The automated pipetting nucleic acid extraction PCR all-in-one machine of claim 1, wherein: manipulator subassembly (4) include first linear electric motor (41) and second linear electric motor (42), the both ends of first linear electric motor (41) respectively with the active cell seat fixed connection of two electric guide (3), the active cell seat leading flank of first linear electric motor (41) all is equipped with second linear electric motor (42), second linear electric motor (42) active cell seat leading flank of front side is equipped with extraction element (10), second linear electric motor (42) active cell seat leading flank of rear side is equipped with sucking disc (11), the output of PLC controller (2) is connected to the equal electricity of input of first linear electric motor (41) and second linear electric motor (42).

3. The automated pipetting nucleic acid extraction PCR all-in-one machine of claim 1, wherein: still include laminating machine (6), laminating machine (6) set up in the upper surface rear side of bottom plate (1), and laminating machine (6) set up with 96 orifice plates (8) cooperation, and the output of PLC controller (2) is connected to the input electricity of laminating machine (6).

4. The automated pipetting nucleic acid extraction PCR all-in-one machine of claim 1, wherein: the PCR instrument (7) is a fluorescent quantitative PCR instrument.

5. The automated pipetting nucleic acid extraction PCR all-in-one machine of claim 1, wherein: the sucker (11) is an electric vacuum sucker, and the input end of the sucker (11) is electrically connected with the output end of the PLC (2).