CN216073836U

CN216073836U - High-flux nucleic acid extraction and purification device

Info

Publication number: CN216073836U
Application number: CN202121553804.6U
Authority: CN
Inventors: 肖腾飞
Original assignee: Shanghai Xunbaihui Biotechnology Co ltd
Current assignee: Shanghai Xunbaihui Biotechnology Co ltd
Priority date: 2021-07-09
Filing date: 2021-07-09
Publication date: 2022-03-18
Anticipated expiration: 2031-07-09

Abstract

The utility model relates to the technical field of gene sequencing instruments, in particular to a high-flux nucleic acid extraction and purification device which comprises a base, wherein a liquid injection area and a purification area are respectively arranged at two ends of the base, a cracking mechanism is arranged on the liquid injection area of the base, and a purification mechanism is arranged on the purification area of the base. Wherein, the cracking mechanism is used for cracking the cells to release nucleic acid and dispensing; and the purification mechanism transfers the magnetic beads adsorbed with the nucleic acid by liquid adding, liquid transferring or magnetic force mechanisms, and the purification mechanism is matched with the magnetic beads adsorbed with the nucleic acid to realize the purification of the nucleic acid. The high-flux nucleic acid extraction and purification device can realize automatic extraction and purification of nucleic acid so as to improve efficiency and ensure accuracy of results.

Description

High-flux nucleic acid extraction and purification device

Technical Field

The utility model relates to the technical field of gene sequencing instruments, in particular to a high-throughput nucleic acid extraction and purification device.

Background

Nucleic acids are carriers of genetic information, are the most important biological information molecules, and are the main objects of molecular biological research. Nucleic acid extraction is also the most critical operation in clinical molecular diagnosis, and is directly related to success or failure of experiments, and is a step of clinical molecular diagnosis which is easy to cause problems. At present, the extraction and purification processes of nucleic acid are generally operated manually, the operation steps are more complicated and error is easy to make, the sample adding amount is not easy to control, the difference of detection results of the same batch of samples and different batches of samples is increased, the efficiency of manual operation is limited, and the sequencing efficiency is lower.

SUMMERY OF THE UTILITY MODEL

In order to solve the above technical problems, an object of the present invention is to provide a high-throughput nucleic acid extraction and purification apparatus, which can automatically extract and purify nucleic acid, thereby improving efficiency and ensuring accuracy of results.

In order to achieve the technical effect, the utility model adopts the following technical scheme:

a high-flux nucleic acid extraction and purification device comprises a base, wherein a liquid injection area and a purification area are respectively arranged at two ends of the base, a cracking mechanism is arranged on the liquid injection area of the base and comprises a shaking table and a mounting seat, a cracking groove is formed in the shaking table and is arranged right above the liquid injection area, the cracking groove is connected with a separate injection head through a separate injection pipeline, the separate injection head is slidably mounted at the bottom of the mounting seat, and a y-axis driving piece used for driving the separate injection head to move along the y-axis direction is arranged on the mounting seat; the base is provided with a purification mechanism in a purification area, the purification mechanism comprises a magnetic rod assembly and a liquid transfer assembly, the magnetic rod assembly comprises a magnetic rack and a first driving piece used for driving the magnetic rack to slide along the base, and the liquid transfer assembly comprises a liquid transfer head and a second driving piece used for driving the liquid transfer head to slide along the base; the base is also provided with a sample seat and an x-axis driving piece for driving the sample seat to move between the liquid injection area and the purification area, the sample seat is detachably provided with a porous sample plate, and the porous sample plate is provided with a plurality of groups of sample grooves along the directions of an x axis and a y axis; the base is further provided with a controller, and the cracking mechanism, the purifying mechanism and the x-axis driving piece are electrically connected with the controller.

Further, the liquid transfer head comprises a liquid transfer support and a plurality of pipettes arranged at the bottom of the liquid transfer support in a lifting mode, a negative pressure assembly is further arranged on the base, the pipettes are connected with the negative pressure assembly through negative pressure pipelines, and electromagnetic valves are further arranged on the negative pressure pipelines.

Further, the magnetic rod assembly comprises a magnetic rod support, and a plurality of magnetic core rods are arranged at the bottom of the magnetic rod support in a liftable mode.

Further, first driving piece and second driving piece are electronic ball screw, slidable mounting has the drive table on the electronic ball screw, move liquid head and magnetic frame and all correspond and install on the drive table, just first driving piece and second driving piece respectively fixed mounting in the both sides of base.

Further, the base is in purification region keeps away from the regional one end of annotating liquid still is equipped with waste liquid groove, reagent groove and washing cistern, the second driving piece can drive move the liquid head and move extremely waste liquid groove, reagent groove and washing liquid trench position.

Furthermore, the bottom of the mounting seat is connected with a driving seat in a sliding manner, and the dispensing head is fixedly mounted on the driving seat; the top of the driving seat is provided with a rack, the mounting seat is provided with a gear meshed with the rack, and the mounting seat is internally provided with a power mechanism used for driving the gear to rotate intermittently.

Furthermore, the power mechanism comprises a first motor arranged in the mounting seat, the output end of the first motor is provided with a driving thumb wheel, one end of the gear is coaxially provided with a grooved wheel, and the grooved wheel is matched with the driving thumb wheel and serves as a transmission piece.

Further, the x-axis driving part comprises a linear guide rail fixedly connected with the base, a linear sliding table is slidably mounted on the linear guide rail, and the sample seat is fixedly mounted on the linear sliding table.

Compared with the prior art, the utility model has the beneficial effects that: the utility model provides a high-flux nucleic acid extraction and purification device, which is provided with a base, wherein a liquid injection area and a purification area are respectively arranged at two ends of the base, a cracking mechanism and a purification mechanism are respectively arranged in the liquid injection area and the purification area, and an x-axis driving piece for driving a sample seat to transfer between the liquid injection area and the purification area is arranged on the base. When the device is implemented, cells can be cracked by the cracking mechanism to release nucleic acid and the lysate is distributed into different sample tanks, the purification mechanism transfers magnetic beads attached with the nucleic acid by liquid adding, liquid transferring or magnetic force mechanisms, and the magnetic rod assembly and the liquid transferring assembly are matched with each other to realize automatic extraction and purification of the nucleic acid, so that the efficiency is improved, and the accuracy of results is ensured.

Drawings

FIG. 1 is a schematic diagram of an overall structure of a high throughput nucleic acid extraction and purification apparatus according to an embodiment of the present invention;

FIG. 2 is a top view of a high throughput nucleic acid extraction and purification apparatus according to one embodiment of the present invention;

FIG. 3 is a schematic cross-sectional view taken along line A-A of a high throughput nucleic acid extraction and purification apparatus according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of a partial enlarged structure at A of a high throughput nucleic acid extraction and purification apparatus according to an embodiment of the present invention;

the reference signs are: 10, a base, 20, a linear guide rail, 21, a linear sliding table, 22, a sample holder, 23, a multi-hole sample plate, 231, a sample groove, 30, a support base, 31, a mounting base, 32, a shaking table, 321, a lysis groove, 40, a driving base, 41, a dispensing pipeline, 42, a dispensing head, 43, a rack, 44, a gear, 45, a first motor, 451, a reducer, 452, a driving dial wheel, 453, a grooved wheel, 50, an electric ball screw, 51, a driving table, 60, a magnetic rod support, 61, a magnetic core rod, 70, a pipetting support, 71, a pipette, 81, a waste liquid groove, 82, a reagent groove, 83, and a cleaning liquid groove.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

As shown in fig. 1 to 4, the high-throughput nucleic acid extraction and purification device provided in this embodiment includes a base 10, a liquid injection region and a purification region are respectively disposed at two ends of the base 10, the base 10 is provided with a cracking mechanism in the liquid injection region, and the base 10 is provided with a purification mechanism in the purification region. Specifically, the lysis mechanism is used for lysing cells to release nucleic acids, and dispensing a cell lysate containing the nucleic acids into different sample cells 231; and the purification mechanism transfers the magnetic beads adsorbed with the nucleic acid by liquid adding, liquid transferring or magnetic force mechanisms, and the purification mechanism is matched with the magnetic beads adsorbed with the nucleic acid to realize the purification of the nucleic acid. Meanwhile, in order to facilitate the transfer of the sample between the priming area and the purification area, a sample holder 22 and an x-axis driving member for driving the sample holder 22 to move between the priming area and the purification area are further disposed on the base 10, and a multi-hole sample plate 23 is detachably disposed on the sample holder 22, and a plurality of sets of sample grooves 231 are disposed on the multi-hole sample plate 23 along the x-axis direction and the y-axis direction, so as to achieve high throughput extraction and purification of the nucleic acid. Still be equipped with the controller on the base 10, cracking mechanism, purification mechanism and x axle driving piece all with the controller electricity is connected so that carry out automatic control to extracting and purification process, can realize the automatic extraction and the purification of nucleic acid basically, with reduction artifical intensity of labour.

In this embodiment, the lysis mechanism comprises a shaking table 32 and a mounting seat 31, the mounting seat 31 is fixedly connected to the base 10 through a supporting seat 30, the shaking table 32 is installed directly above the mounting seat 31, and a lysis trough 321 is disposed on the shaking table 32, and the lysis trough 321 is used for adding reagents and cells to facilitate extraction of nucleic acids in the cells. A shaking table 32 mechanism is provided in the mounting seat 31 so as to drive the shaking table 32 to shake, thereby accelerating the extraction process of nucleic acid. Simultaneously, after the extraction process is accomplished, for replacing the manual work to the sample application of separately annotating, mount pad 31 is located annotate the liquid region directly over, schizolysis groove 321 is connected with branch and annotates head 42 through annotating branch and annotate pipeline 41, it just to be equipped with the peristaltic pump on the pipeline 41 to annotate the peristaltic pump with the controller electricity is connected, and branch annotate head 42 then slidable mounting in mount pad 31's bottom just be equipped with on the mount pad 31 and be used for the drive divide annotate the y axle driving piece of head 42 along the motion of y axle direction. In specific implementation, the y-axis driving member drives the dispensing head 42 to slide along the y-direction, and meanwhile, the x-axis driving member and the peristaltic pump are matched to dispense and sample-add the plurality of sample slots 231 on the multi-hole sample plate 23, so that the accuracy and the efficiency are high.

In this embodiment, a driving seat 40 is slidably connected to the bottom of the mounting seat 31, and the dispensing head 42 is fixedly mounted on the driving seat 40; the top of the driving seat 40 is provided with a rack 43, the mounting seat 31 is provided with a gear 44 engaged with the rack 43, and the mounting seat 31 is internally provided with a power mechanism for driving the gear 44 to rotate intermittently. Specifically, the power mechanism includes a first motor 45 disposed in the mounting seat 31, and the first motor 45 is electrically connected to the controller. The output end of the first motor 45 is connected with a driving thumb wheel 452 through a decelerator 451, and one end of the gear 44 is coaxially provided with a grooved wheel 453, and the grooved wheel 453 is engaged with the driving thumb wheel 452 and serves as a transmission member. In specific implementation, the controller controls the driving dial 452 to rotate, so that the grooved wheel 453 is driven by the driving dial 452 to rotate intermittently, the gear 44 rotates synchronously with the grooved wheel 453, and the rack 43 can move a fixed distance each time under the driving of the gear 44, and the distance is equal to the distance between two adjacent sample slots 231 in the y direction.

In this embodiment, in order to facilitate automatic processing of the filled sample in the sample tank 231, the purification mechanism includes a magnetic bar assembly and a pipetting assembly, the magnetic bar assembly includes a magnetic rack and a first driving member for driving the magnetic rack to slide along the base 10, and the pipetting assembly includes a pipetting head and a second driving member for driving the pipetting head to slide along the base 10. Meanwhile, the base 10 is in the purification area is kept away from the one end of annotating the liquid area and still is equipped with waste liquid groove 81, reagent groove 82 and washing cistern 83, the second driving piece can drive move the liquid head to waste liquid groove 81, reagent groove 82 and washing cistern 83 position. Specifically, the pipetting head includes pipetting support 70 and locate with liftable a plurality of pipettes 71 of pipetting support 70 bottom, still be equipped with negative pressure assembly on the base 10, pipette 71 pass through negative pressure pipeline with negative pressure assembly connects, still be equipped with the solenoid valve on the negative pressure pipeline. The magnetic rod assembly comprises a magnetic rod bracket 60, and a plurality of magnetic core rods 61 are arranged at the bottom of the magnetic rod bracket 60 in a lifting manner. More specifically, the pipette 71 is connected to the pipetting support 70 by a first lifting drive, the magnetic core rod 61 is connected to the magnetic rod support 60 by a second lifting drive, and both the first lifting drive and the second lifting drive can be selected from the prior art. And first driving piece and second driving piece are electronic ball screw 50, slidable mounting has drive table 51 on electronic ball screw 50, it all corresponds to install to move liquid head and magnetic frame on drive table 51, first driving piece and second driving piece respectively fixed mounting in the both sides of base 10. In specific implementation, the first driving member and the second driving member respectively drive the magnetic rack and the pipetting head to move along the base 10 under the control of the controller, and respectively process the magnetic beads and the reagent in the sample slot 231 under the cooperation of the first lifting driving member and the second lifting driving member. Meanwhile, in order to facilitate the pipetting head to transfer the sample, an external magnetic field is further arranged on the base 10.

In this embodiment, in order to facilitate driving the movement of the sample holder 22, the x-axis driving component includes a linear guide 20 fixedly connected to the base 10, a linear sliding table 21 is slidably mounted on the linear guide 20, and the sample holder 22 is fixedly mounted on the linear sliding table 21, and in specific implementation, the linear sliding table 21 is driven by the linear guide 20 to move, so as to transfer the porous sample plate 23 on the sample holder 22 in the liquid injection area and the purification area.

Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the utility model as defined in the appended claims. The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims

1. A high flux nucleic acid extraction and purification device is characterized in that: the liquid injection device is characterized by comprising a base (10), wherein liquid injection regions and purification regions are respectively arranged at two ends of the base (10), a cracking mechanism is arranged in the liquid injection region of the base (10), the cracking mechanism comprises a shaking table (32) and a mounting seat (31), a cracking groove (321) is arranged on the shaking table (32), the mounting seat (31) is arranged right above the liquid injection region, the cracking groove (321) is connected with a dispensing head (42) through a dispensing pipeline (41), the dispensing head (42) is slidably mounted at the bottom of the mounting seat (31), and a y-axis driving piece used for driving the dispensing head (42) to move along the y-axis direction is arranged on the mounting seat (31); the base (10) is provided with a purifying mechanism in a purifying area, the purifying mechanism comprises a magnetic rod assembly and a liquid transferring assembly, the magnetic rod assembly comprises a magnetic rack and a first driving piece used for driving the magnetic rack to slide along the base (10), and the liquid transferring assembly comprises a liquid transferring head and a second driving piece used for driving the liquid transferring head to slide along the base (10); the base (10) is also provided with a sample seat (22) and an x-axis driving piece for driving the sample seat (22) to move between the liquid injection area and the purification area, the sample seat (22) is detachably provided with a porous sample plate (23), and the porous sample plate (23) is provided with a plurality of groups of sample grooves (231) along the x-axis direction and the y-axis direction; the base (10) is further provided with a controller, and the cracking mechanism, the purifying mechanism and the x-axis driving piece are electrically connected with the controller.

2. The high throughput nucleic acid extraction and purification apparatus of claim 1, wherein: the liquid transfer head comprises a liquid transfer support (70) and a plurality of pipettes (71) arranged at the bottom of the liquid transfer support (70) in a lifting mode, a negative pressure assembly is further arranged on the base (10), the pipettes (71) are connected with the negative pressure assembly through negative pressure pipelines, and electromagnetic valves are further arranged on the negative pressure pipelines.

3. The high throughput nucleic acid extraction and purification apparatus of claim 1, wherein: the magnetic rod assembly comprises a magnetic rod support (60), and a plurality of magnetic core rods (61) are arranged at the bottom of the magnetic rod support (60) in a lifting mode.

4. The high throughput nucleic acid extraction and purification apparatus of claim 1, wherein: first driving piece and second driving piece are electronic ball screw (50), slidable mounting has drive table (51) on electronic ball screw (50), it all corresponds to install to move liquid head and magnetic frame drive table (51) is last, just first driving piece and second driving piece respectively fixed mounting in the both sides of base (10).

5. The high throughput nucleic acid extraction and purification apparatus of claim 1, wherein: the base (10) is in the purification region is kept away from the one end in annotating the liquid region and still is equipped with waste liquid groove (81), reagent groove (82) and washing cistern (83), the second driving piece can drive move the liquid head and move to waste liquid groove (81), reagent groove (82) and washing cistern (83) position.

6. The high throughput nucleic acid extraction and purification apparatus of claim 1, wherein: the bottom of the mounting seat (31) is connected with a driving seat (40) in a sliding manner, and the dispensing head (42) is fixedly mounted on the driving seat (40); the top of drive seat (40) is equipped with rack (43), be equipped with on mount pad (31) with gear (44) of rack (43) meshing, be equipped with in mount pad (31) and be used for driving gear (44) intermittent type pivoted power unit.

7. The high throughput nucleic acid extraction and purification apparatus of claim 6, wherein: the power mechanism comprises a first motor (45) arranged in the mounting seat (31), an output end of the first motor (45) is provided with a driving thumb wheel (452), one end of the gear (44) is coaxially provided with a grooved wheel (453), and the grooved wheel (453) is matched with the driving thumb wheel (452) and serves as a transmission piece.

8. The high throughput nucleic acid extraction and purification apparatus of claim 1, wherein: the x-axis driving piece comprises a linear guide rail (20) fixedly connected with the base (10), a linear sliding table (21) is slidably mounted on the linear guide rail (20), and the sample seat (22) is fixedly mounted on the linear sliding table (21).