CN201262610Y - Fluorescent quantitative PCR detection system based on bottom scanning detection - Google Patents
Fluorescent quantitative PCR detection system based on bottom scanning detection Download PDFInfo
- Publication number
- CN201262610Y CN201262610Y CNU2008201217449U CN200820121744U CN201262610Y CN 201262610 Y CN201262610 Y CN 201262610Y CN U2008201217449 U CNU2008201217449 U CN U2008201217449U CN 200820121744 U CN200820121744 U CN 200820121744U CN 201262610 Y CN201262610 Y CN 201262610Y
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- metal module
- detection system
- alternating temperature
- temperature metal
- quantitative pcr
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Abstract
The utility model discloses a fluorescence quantitative PCR detection system which is based on the scan and detection of the bottom part, comprising a temperature-variable metal module, a semiconductor refrigerator and a heat radiation device, which can be arranged from top to down in sequence into a whole, wherein, the temperature-variable metal module can be inserted by a plurality of test tubes. The detection system is characterized in that the test tube jack of the temperature-variable metal module is a through-hole communicated with the temperature-variable metal module, the semiconductor refrigerator and the heat radiation device; the heat radiation device is a radiator of a heat pipe; the heat pipe extends to the outer side of the whole structure and the extending end is connected with a radiating fin; a photoelectric detector is positioned at the bottom part of the whole-structure heat radiation device and scanned under the drive of a motor; and the fluorescence of a reagent is detected from the bottom of the test tubes. By using the utility model, the efficiency of exciting light can be enhanced, higher fluorescence sensitivity and fluorescence signal strength are obtained, and the stability is improved. The detection result is more accurate, the rapid temperature increase and decrease and precise temperature control of the temperature-variable metal module can be achieved, and the service life of the temperature-variable metal module is greatly prolonged.
Description
Technical field
The utility model relates to fluorescence quantitative PCR detection system, especially the fluorescence quantitative PCR detection system that detects based on bottom scan.
Background technology
The fluorescence quantitative PCR detection system principle of work is the instrument that makes reactant Automatic Cycle between denaturation temperature, renaturation temperature and the elongating temperature of appointment, and the temperature cycles by sex change, renaturation and extension can increase millions of times with target DNA at short notice.
The main flow fluorescence quantitative PCR detection system comprises from top to bottom the alternating temperature metal module, semiconductor cooler, radiating element and the blower fan that insert some test tubes of device successively at present.Adopt the semiconductor cooler alternating temperature, the temperature follow procedure of alternating temperature metal module is heated up and cooling, be positioned over the invisible spectro reactant of metal module by the temperature required circulation change of PCR thereby make.
Fluorescence quantitative PCR detection system is by using the excitation light irradiation test tube of different wave length, when the reagent in the test tube was inspired fluorescence, optical sensor (PMT, PD, CCD etc.) collected fluorescence intensity signals and in time is sent to computing machine and carries out real time data demonstration and analysis.
The mode of operation of main flow fluorescence quantitative PCR detection system (Real time PCR) has at present:
(a) top detecting pattern (see figure 1).Most systems all adopts this pattern, it is characterized in that: alternating temperature metal module bottom seal, and excitation source all is positioned at the module top, and optical detection device also is positioned at the module top, general scioptics focusing or with the scan mode image data.Under this pattern, can use general semiconductor cooler, heating radiator.But shortcoming is: because the out-of-flatness of plastic test tube top often has evaporation and dewfall steam to stop and drippage, cause the fluorescence signal sudden change and detect repeated variation.When detecting from the top simultaneously, distance is far away, causes exciting light to die down and signal weaker.
(b) side detecting pattern (see figure 2).Peer machine adopts this pattern, it is characterized in that: alternating temperature metal module bottom is sealed, excitation source from top incident or with the fluorescence signal common optical fiber, the optical fiber light-guiding bundle that fluorescence signal is drawn by side, module hole conducts.Under this pattern, the influence of plastic test tube can reduce, and is easily polluted, weares and teares, ruptures by residue but optical fiber is positioned at sidewalls, and be difficult to cleaning, uses the limited and lost of life.A little less than believing that signal relatively under this pattern, system stability is poor.And optical fiber is buried underground and keeps in repair very difficult.
Summary of the invention
At the defective that prior art exists, the purpose of this utility model provides the fluorescence quantitative PCR detection system based on the bottom scan detection of a kind of high sensitivity, high stability.
The fluorescence quantitative PCR detection system that detects based on bottom scan of the present utility model, comprise and install the alternating temperature metal module that inserts some test tubes in aggregates from top to bottom successively, semiconductor cooler and radiating element, the test tube jack that it is characterized in that the alternating temperature metal module is for connecting the alternating temperature metal module, the through hole of semiconductor cooler and radiating element, radiating element is a heat-pipe radiator, heat pipe extends to the one-piece construction outside, elongated end connects radiating fin, photoelectric detector is positioned at the bottom of one-piece construction radiating element, by motor-driven scanning, from the floor detection reagent fluorescence of test tube.
Further feature of the present utility model is that alternating temperature metal module bottom has the circular protrusion around the test tube hole, heat-pipe radiator is made of copper billet and heat pipe, heat pipe one end is imbedded in the copper billet, the other end outwards leads to the connection radiating fin, be furnished with on the copper billet and test tube hole through hole one to one, on copper billet, have and the corresponding concave surface of alternating temperature metal module bottom circular protrusion around the test tube hole, the jut of copper billet top is connected with semiconductor cooler, concave surface is connected with alternating temperature metal module bottom circular protrusion by heatproof O-ring seal disposed thereon or sealing gluing, so not only thermal insulation but also airtight, condensation vapor and influence refrigerator work in the air in the time of can preventing low temperature.
The copper billet plane contacts with the hot side of semiconductor cooler, plays the effect of heat conduction and heat radiation.
Has heat transfer medium between alternating temperature metal module and the semiconductor cooler and between semiconductor cooler and the radiating element.Said heat transfer medium can be that glue or heat conduction phase-change material or conductive graphite etc. are solidified in heat conduction.
For strengthening radiating effect, further feature of the present utility model is that blower fan is installed on radiating fin.
The beneficial effects of the utility model are:
1) because heat pipe has very good thermal conduction capability, and the liquid working substance of inside heat pipe can be transmitted to a large amount of heats from refrigerator position far away, both sides, and by wind-cooling heat dissipating, fluoroscopic examination mechanism can be placed in the space that the below is reserved.The utility model adopts bottom scan, floor detection reagent fluorescence from test tube, can residual liquid in the hole, eliminated the emptying aperture fluorescence background problem under the common detecting pattern, make testing result more accurate, and prolonged serviceable life of alternating temperature metal module greatly, make cleaning become low cost and low-risk simultaneously.
2) adopt bottom scan, photoelectric detector and test tube distance from bottom can be adjusted to minimum, help improving exciting light efficient, obtain higher fluorescence sensitivity and fluorescence signal intensity, improve stability.
3) allow to use all kinds of test tubes, no matter height, flat-top or dome test tube, or skirtboard type test tube is all unrestricted, all can obtain desired result.Avoid in vitro liquid evaporation and the stop of dewfall steam and dripped the signal fluctuation that causes.
4) have anti-condensation water function, allow the long-time running of alternating temperature metal module working temperature to be less than or equal to 4 ℃, can realize sample deepfreeze function in the test tube.
5) have metastable heat dissipation environment, can realize the gentle precision temperature control of fast lifting of alternating temperature metal module.
Description of drawings
Fig. 1 is a top detecting pattern synoptic diagram;
Fig. 2 is a side detecting pattern synoptic diagram;
Fig. 3 is based on the fluorescence quantitative PCR detection system synoptic diagram that bottom scan detects;
Fig. 4 is the alternating temperature metal module of Fig. 3, the one-piece construction enlarged drawing that semiconductor cooler and radiating element are installed;
Fig. 5 is the sectional axonometric drawing of Fig. 3 alternating temperature metal module.
Embodiment
Further specify the utility model below in conjunction with accompanying drawing.
With reference to Fig. 3, Fig. 4, Fig. 5, the fluorescence quantitative PCR detection system that detects based on bottom scan of the present utility model, comprise and install the alternating temperature metal module 1 that inserts some test tubes in aggregates from top to bottom successively, semiconductor cooler 2 and radiating element 3, the test tube jack of alternating temperature metal module is for connecting the through hole of alternating temperature metal module 1 and semiconductor cooler 2 and radiating element 3.Radiating element 3 is a heat-pipe radiator, in the illustrated example, heat-pipe radiator 3 is made of copper billet and heat pipe, heat pipe one end is imbedded in the copper billet, the other end is outwards drawn and is extended to the one-piece construction outside, the radiating fin 5 of elongated end connecting band blower fan, be furnished with on the copper billet and test tube hole through hole one to one, on copper billet, the concave surface corresponding with alternating temperature metal module bottom circular protrusion 6 arranged around the test tube hole, the jut of copper billet top is connected with semiconductor cooler 2, and concave surface is connected with alternating temperature metal module bottom circular protrusion 6 by heatproof O-ring seal disposed thereon or sealing gluing 7.
Has heat transfer medium 4 between alternating temperature metal module 1 and the semiconductor cooler 2 and between semiconductor cooler 2 and the radiating element 3, for example glue or heat conduction phase-change material or conductive graphite etc. are solidified in heat conduction, semiconductor cooler is connected with alternating temperature metal module and radiating element, can adopt securing member to connect or employing heat-conducting glue hot setting.Photoelectric detector 8 is positioned at the bottom of one-piece construction radiating element 3, by motor 9 driven sweeps, from the floor detection reagent fluorescence of test tube.
Claims (5)
1. fluorescence quantitative PCR detection system that detects based on bottom scan, comprise and install the alternating temperature metal module (1) that inserts some test tubes in aggregates from top to bottom successively, semiconductor cooler (2) and radiating element (3), the test tube jack that it is characterized in that the alternating temperature metal module is for connecting alternating temperature metal module (1), the through hole of semiconductor cooler (2) and radiating element (3), radiating element (3) is a heat-pipe radiator, heat pipe extends to the one-piece construction outside, elongated end connects radiating fin (5), photoelectric detector (8) is positioned at the bottom of one-piece construction radiating element (3), by motor (9) driven sweep, from the floor detection reagent fluorescence of test tube.
2. fluorescence quantitative PCR detection system according to claim 1 is characterized in that on the radiating fin (5) blower fan being installed.
3. fluorescence quantitative PCR detection system according to claim 1, it is characterized in that alternating temperature metal module bottom has the circular protrusion (6) around the test tube hole, heat-pipe radiator (3) is made of copper billet and heat pipe, heat pipe one end is imbedded in the copper billet, the other end outwards leads to the connection radiating fin, be furnished with on the copper billet and test tube hole through hole one to one, on copper billet, have and the corresponding concave surface of alternating temperature metal module bottom circular protrusion around the test tube hole, the jut of copper billet top is connected with semiconductor cooler (2), and concave surface is connected with alternating temperature metal module bottom circular protrusion by heatproof O-ring seal disposed thereon or sealing gluing.
4. fluorescence quantitative PCR detection system according to claim 1 and 2 is characterized in that having heat transfer medium (4) between alternating temperature metal module (1) and the semiconductor cooler (2) and between semiconductor cooler (2) and the radiating element (3).
5. fluorescence quantitative PCR detection system according to claim 4 is characterized in that said heat transfer medium (4) solidifies glue or heat conduction phase-change material or conductive graphite for heat conduction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201217449U CN201262610Y (en) | 2008-07-18 | 2008-07-18 | Fluorescent quantitative PCR detection system based on bottom scanning detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201217449U CN201262610Y (en) | 2008-07-18 | 2008-07-18 | Fluorescent quantitative PCR detection system based on bottom scanning detection |
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| Publication Number | Publication Date |
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| CN201262610Y true CN201262610Y (en) | 2009-06-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201217449U Expired - Lifetime CN201262610Y (en) | 2008-07-18 | 2008-07-18 | Fluorescent quantitative PCR detection system based on bottom scanning detection |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329725A (en) * | 2010-06-16 | 2012-01-25 | 三星泰科威株式会社 | Light transmittable temperature control device and polymerase chain reaction apparatus with the same |
| CN101698823B (en) * | 2009-10-23 | 2012-07-04 | 西安天隆科技有限公司 | High-speed servo motor and optical positioning-based fluorescence quantitative PCR detection system |
| CN104136914A (en) * | 2011-12-15 | 2014-11-05 | 海因生命科学股份有限公司 | A device for optically measuring fluorescence of nucleic acids in test samples and use of the device |
| CN111263885A (en) * | 2017-09-19 | 2020-06-09 | 拜克门寇尔特公司 | System for analog light measurement and photon counting in chemiluminescence measurement |
-
2008
- 2008-07-18 CN CNU2008201217449U patent/CN201262610Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101698823B (en) * | 2009-10-23 | 2012-07-04 | 西安天隆科技有限公司 | High-speed servo motor and optical positioning-based fluorescence quantitative PCR detection system |
| CN102329725A (en) * | 2010-06-16 | 2012-01-25 | 三星泰科威株式会社 | Light transmittable temperature control device and polymerase chain reaction apparatus with the same |
| CN104136914A (en) * | 2011-12-15 | 2014-11-05 | 海因生命科学股份有限公司 | A device for optically measuring fluorescence of nucleic acids in test samples and use of the device |
| CN104136914B (en) * | 2011-12-15 | 2016-08-24 | 海因生命科学股份有限公司 | The device of fluorescence and the use of this device for optical measurement sample amplifying nucleic acid |
| CN111263885A (en) * | 2017-09-19 | 2020-06-09 | 拜克门寇尔特公司 | System for analog light measurement and photon counting in chemiluminescence measurement |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20090624 |