CN103969236A - Portable pathogen detector - Google Patents
Portable pathogen detector Download PDFInfo
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- CN103969236A CN103969236A CN201410191840.0A CN201410191840A CN103969236A CN 103969236 A CN103969236 A CN 103969236A CN 201410191840 A CN201410191840 A CN 201410191840A CN 103969236 A CN103969236 A CN 103969236A
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Abstract
The invention discloses a pathogen detector. The pathogen detector comprises a transparent amplified reaction chip, a temperature controller, a heater, a radiator, a fluorescence excitation unit and a fluorescence receiving unit, wherein the amplified reaction chip is provided with a reaction hole and is embedded on the heater; the temperature controller is arranged between the amplified reaction chip and the heater; the radiator is arranged below the heater; the fluorescence excitation unit is vertically arranged above the reaction hole of the amplified reaction chip; the fluorescence receiving unit is arranged above the reaction hole of the amplified reaction chip and forms an angle alpha together with the fluorescence excitation unit; the angle alpha ranges from 0 to 80 degrees. The pathogen detector is compact in structure, small in size, convenient to carry, low in cost, simultaneously good in constant temperature control effect and high in detection precision, and can be applied to bulk detection.
Description
Technical field
The invention belongs to medical science detection field, more specifically, relate to a kind of Portable disease substance detector.
Background technology
Nucleic acid amplification is one of method the most frequently used in gene diagnosis technology, the method of nucleic acid amplification is a lot of at present, but all because having shortcoming at aspects such as specificity, easy degree, temperature and the requirements of reagent instrument, be seldom applied in clinical diagnosis and the on-the-spot occasion detecting in real time.The method has simply, fast, the easily advantage such as detection of the high and product of high specificity, amplification efficiency.
When the principle of fluoroscopic examination is the irradiation of microorganism or the certain wavelength of compound quilt, they can be stimulated and send fluorescence, measure the fluorescent energy sending and are fluoroscopic examination.Different materials generally only absorbs the light of specific wavelength, and produces the fluorescence of specific wavelength, so fluorescence detection method is differentiated very effective for the specificity of material.As some pathogenic bacteria or virus in the time being subject to UV-irradiation, can produce fluorescence, this is autofluorescence method.The nucleic acid molecules of some germ or pathogen itself can't produce fluorescence in the time of irradiation, but they can adsorb specific fluorescent dye or pigment, these fluorescent dyes or pigment can produce the fluorescence of specific wavelength under the fluorescence of specific wavelength irradiates, and this is indirect fluorescent method.Fluoroscopic examination, as a kind of detection method conventional in biomedicine, has the features such as sensitivity height, is widely used in the detection of faint fluorescence.
Current fluoroscopic examination analytical instrument, as PCR instrument and qPCR instrument, volume is large, price, reagent and operating personnel are required high, and these shortcomings determine that it can not be used in portability field.Highly integrated, portability, the high throughput analysis of instrument and detect robotization and become an important developing direction.Environmental pollution, the problem serious threats such as water pollution and food security human health and life, the fluoroscopic examination of microorganism, pathogen, toxic compounds, the even analysis to water quality, the analysis of food composition, all in the urgent need to invent a kind of easy to carry, detect instrument accurately and rapidly.
Summary of the invention
For above defect or the Improvement requirement of prior art, the invention provides a kind of pathogen detection instrument, its object is by compact design and selects its all parts, solves thus current pathogen detection instrument volume large, carries inconvenience, the technical matters that cost is expensive.
For achieving the above object, according to one aspect of the present invention, provide a kind of pathogen detection instrument, comprised transparent amplified reaction chip, temperature controller, well heater, heating radiator, fluorescence excitation unit and fluorescence reception unit; Described amplified reaction chip is provided with reacting hole, described amplified reaction chip is entrenched on described well heater, between described amplified reaction chip and described well heater, be provided with temperature control device, described well heater has heating radiator, fluorescence excitation unit is vertically installed in described amplified reaction chip reacting hole top, described fluorescence reception unit is arranged at amplified reaction chip reacting hole top and is angle α with described fluorescence excitation unit, angle α at 10 degree between 80 degree.
Preferably, pathogen detection instrument, it is characterized in that, on described amplified reaction chip, there are multiple reacting holes, described fluorescence excitation unit and fluorescence reception unit are arranged on one of them reacting hole top, described amplified reaction chip and described fluorescence excitation unit and fluorescence reception unit can be movable relatively, and make reacting hole successively in described fluorescence excitation unit and below, fluorescence reception unit.
Preferably, described pathogen detection instrument, the material of its amplified reaction chip is glass.
Preferably, described pathogen detection instrument, its well heater is Peltier.
Preferably, described pathogen detection instrument, its heat abstractor is passive radiator.
Preferably, described pathogen detection instrument, in its fluorescence excitation unit and fluorescence reception unit, at least one comprises optical filter.
Preferably, described pathogen detection instrument, its fluorescence excitation unit comprises light source and short focal length focusing len, described light source sends fluorescent exciting, focuses on through too short burnt condenser lens, projects on the sample in the reacting hole of amplified reaction chip.
Preferably, described pathogen detection instrument, its fluorescence reception unit comprises short focal length focusing len and photoelectric tube, the sample in the reacting hole of amplified reaction chip, through excitation, is launched fluorescence, after short focal length focusing len focuses on, is caught by photoelectric tube.
In general, the above technical scheme of conceiving by the present invention compared with prior art, can obtain following beneficial effect:
(1) the present invention adopt micro-fluidic chip and fluorescence excitation unit and the fluorescence reception unit of compact conformation, the volume of instrument own is little, be easy to carry and cost lower.
(2) temperature controller is arranged between well heater and amplified reaction chip, and the temperature of mensuration approaches the true temperature of sample in reacting hole the most, and thermostatic control is effective, and accuracy of detection is high.
(3) the present invention can be applicable to batch detection, easy to use.
Brief description of the drawings
Fig. 1 is structural representation of the present invention;
Fig. 2 is the structural representation of embodiment 1;
Fig. 3 is the amplified reaction chip structure schematic diagram of embodiment 1;
Fig. 4 is temperature controller, well heater and the heat spreader structures schematic diagram of embodiment 1;
Fig. 5 is fluorescence excitation unit and the fluorescence reception cellular construction schematic diagram of embodiment 1;
Fig. 6 is the structural representation of embodiment 2;
Fig. 7 is the amplified reaction chip structure schematic diagram of embodiment 2;
Fig. 8 is temperature controller, well heater and the heat spreader structures schematic diagram of embodiment 2;
Fig. 9 is fluorescence excitation unit and the fluorescence reception cellular construction schematic diagram of embodiment 2;
Figure 10 is the structural representation of embodiment 3;
Figure 11 is the amplified reaction chip structure schematic diagram of embodiment 3;
Figure 12 is temperature controller, well heater and the heat spreader structures schematic diagram of embodiment 3;
Figure 13 is fluorescence excitation unit and the fluorescence reception cellular construction schematic diagram of embodiment 3.
In institute's drawings attached, identical Reference numeral is used for representing identical element or structure, wherein: 1 is amplified reaction chip, 2 is well heater, 3 is heating radiator, 4 is fluorescence excitation unit, 5 is fluorescence reception unit, 6 is temperature controller, 7 is circular turntable, 8 is translation stage, 11 is amplified reaction chip top layer sample introduction layer, 12 is amplified reaction chip top layer responding layer, 13 is amplified reaction chip bottom responding layer, 14 is amplified reaction chip bottom substrate layer, 15 is amplified reaction chip sample holes, 16 is amplified reaction chip reacting hole, 17 is amplified reaction chip sample outlet hole, 41 is fluorescence excitation unit light source, 42 is fluorescence excitation single-element lens, 43 is fluorescence excitation unit optical filter, 51 is fluorescence reception unit light fulgurite, 52 is fluorescence reception single-element lens, 53 is the optical filter of fluorescence reception unit.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.In addition,, in each embodiment of described the present invention, involved technical characterictic just can combine mutually as long as do not form each other conflict.
Pathogen detection instrument provided by the invention, as shown in Figure 1, comprises transparent amplified reaction chip 1, temperature controller 6, well heater 2, heating radiator 3, fluorescence excitation unit 4 and fluorescence reception unit 5.
Described amplified reaction chip 1 sees through exciting light and emergent light preferably for obtaining, and is necessary for transparent amplified reaction chip, can be glass, organic glass, quartz, is preferably glass.Described amplified reaction chip 1 is provided with reacting hole 16, and reacting hole 16 can be one or more; When reacting hole 16 is while being multiple, can realize batch detection.
Preferably, described amplified reaction chip 1 is a kind of microarray reaction chip, comprises top layer sample introduction layer 11, top layer responding layer 12, bottom responding layer 13 and the bottom substrate layer 14 placed from top to bottom; Wherein top layer sample introduction layer and top layer responding layer bonding composition top layer chip, bottom responding layer and bottom substrate layer bonding composition bottom chip; The sample feeding hole 15 arranging in top layer chip and bottom chip, reacting hole 16, sample outlet hole 17, intercommunicating pore and communicating passage are all arranged on the circumference of different radii as the center of circle radially taking the center hole that runs through axis, each self-corresponding intercommunicating pore and the communicating passage discrete fluid runner array that all complete matching forming section are communicated with on every layer of chip after bonding, reacting hole 16 is not connected with any passage, forms isolated array of circular apertures; On the outmost turns circumference of top layer sample introduction layer, molding has a circle instruction to revolve the scale that sliding angle orientation is used, and on the outmost turns circumference on bottom substrate layer, molding has the pointer of a circle correspondence; Between top layer chip and bottom chip for axle is connected, between the two can rotation-sliding.
Preferably, described amplified reaction chip also can comprise from top to bottom place two-layer, upper strata 18 is for being provided with the dimethyl silicone polymer layer of sample holes 15, pipeline and sample outlet hole 16, lower floor 19 is for being provided with the glassy layer of reacting hole 16, dimethyl silicone polymer layer covers on glassy layer, and each reacting hole 16 is communicated with corresponding sample holes 15 and sample outlet hole 17 by supratectal passage.Response sample enters the reacting hole 16 on glassy layer by the sample holes 15 of entering on dimethyl silicone polymer layer, in the interior acceptance detection of reacting hole 16, after having detected, discharges from sample outlet hole 17 by pipeline.
Described temperature controller 6, for detection of amplified reaction chip 1 actual temperature, and carries out or stops heating according to preset temperature control heater.Described temperature controller 6, is preferably programmable thermostat, commonly uses as temperature sensor DS18B20, and temperature-measuring range-55 DEG C～+ 125 DEG C, in the time of-10～+ 85 DEG C, accuracy of temperature control is ± 0.5 DEG C, programmable best result distinguishes that temperature is 0.0625 DEG C.
Described well heater 2 can be resistance wire, Electric radiant Heating Film, heating plate, electric-heating coatings and Peltier, and preferred well heater is Peltier.Peltier is easy to processing, and its power control and easy to adjust, speed of action are fast, long service life, have high reliability and accurate temperature control ability, meet the needs of Programmable Temperature control.
Described heating radiator 3 can be active heat sink or passive radiator, and active heat sink is the heating radiator that needs external energy to drive, as fan, condenser pipe; Passive radiator is the heating radiator that does not need external energy to drive, as heat radiator.Described heating radiator is preferably passive radiator, due to simple in structure, is easy to integrated.Conventional passive sensor is heat radiator, and heat radiator has strengthened the contact area with air, perfect heat-dissipating, and volume is little, drives without additional energy, and Simultaneous Stabilization is good, is easily combined with well heater.
Described fluorescence excitation unit 4 comprises light source 41 and lens 42, also comprises if desired optical filter 43.Described light source 41 preferably adopts miniwatt long-life diode as light source, is preferably LED, and LED is only automatically lighted or manually touched button and light by program in the time detecting, and saves the energy, can also extend the life-span of LED.Described lens 42, preferably adopt short focal length focus lamp, and its focal length between 15mm, can reduce lens center to the distance between reacting hole at 3mm, reduces the volume of instrument.Described optical filter 43 is selected according to fluorescence indicator, conventional calcein is as fluorescence indicator, the fluorescence excitation wave band of calcein is in blue light or ultraviolet, utilizing emitted light is green glow, band center 530nm, it is 470nm blue-ray LED that light source selects wavelength, excites the optical filter of unit to select band pass filter, wavelengths centered 470nm, bandwidth 10nm.The filter plate of receiving element selects high permeability band pass filter, wavelengths centered 535nm, bandwidth 20nm.Described light source 41 sends fluorescent exciting, focuses on through too short burnt condenser lens 42, and mating plate 43 after filtration if desired, projects on the sample in the reacting hole 16 of amplified reaction chip 1.
Described fluorescence reception unit 5, comprises short focal length focusing len 52 and photoelectric tube 51, comprises if desired optical filter 53.Described lens 52, preferably adopt short focal length focus lamp, and its focal length between 15mm, can coordinate fluorescence excitation unit at 3mm like this, reduce lens center to the distance between reacting hole, reduce the volume of instrument.Described photoelectric tube 51, for the fluorescence signal of collecting through lens 52 and optical filter 53 is converted to electric signal, selects volume little, and sensitivity is the high sensitivity PIN photodiode BPW21 of pA level, fast response time, and the range of linearity is good.Described optical filter 53 is selected according to fluorescence indicator.
Described amplified reaction chip 1 is entrenched on described well heater 2, between described amplified reaction chip 1 and described well heater 2, be provided with temperature controller 6, described well heater 2 has heating radiator 3, fluorescence excitation unit 4 is vertically installed in described amplified reaction chip reacting hole 16 tops, described fluorescence reception unit 5 is arranged at amplified reaction chip reacting hole 16 tops and is angle α with described fluorescence excitation unit 4, angle α between 80 degree, is preferably 45 degree at 10 degree.Described fluorescent emission unit 4 and fluorescence reception unit 5, the focal length that the distance of its lens distance reacting hole is selected lens.In the time having multiple reacting hole on described amplified reaction chip 1, described fluorescence excitation unit 4 and fluorescence reception unit 5 are arranged on one of them reacting hole 16 top, described amplified reaction chip 1 can be movable relatively with described fluorescence excitation unit 4 and fluorescence reception unit 5, makes multiple reacting holes 16 successively in described fluorescence excitation unit 4 and 5 belows, fluorescence reception unit.
Be below embodiment:
Embodiment 1
A kind of pathogen detection instrument, as shown in Figure 2, comprises transparent amplified reaction chip 1, temperature controller, well heater 2, heating radiator 3, fluorescence excitation unit 4 and fluorescence reception unit 5.
Described amplified reaction chip 1, as shown in Figure 3, is glass material, is a kind of microarray reaction chip, comprises top layer sample introduction layer 11, top layer responding layer 12, bottom responding layer 13 and the bottom substrate layer 14 placed from top to bottom; Wherein top layer sample introduction layer 11 and top layer responding layer 12 bonding composition top layer chips, bottom responding layer 13 and bottom substrate layer 14 bonding composition bottom chip; The sample feeding hole 15 arranging in top layer chip and bottom chip, reacting hole 16, sample outlet hole 17, intercommunicating pore and communicating passage are all arranged on the circumference of different radii as the center of circle radially taking the center hole that runs through axis, each self-corresponding intercommunicating pore and the communicating passage discrete fluid runner array that all complete matching forming section are communicated with on every layer of chip after bonding, reacting hole 16 is not connected with any passage, forms isolated array of circular apertures; On the outmost turns circumference of top layer sample introduction layer, molding has a circle instruction to revolve the scale that sliding angle orientation is used, and on the outmost turns circumference on bottom substrate layer, molding has the pointer of a circle correspondence; Between top layer chip and bottom chip for axle is connected, between the two can rotation-sliding.
Described temperature controller 7, as shown in Figure 4, is temperature sensor DS18B20, temperature-measuring range-55 DEG C～+ 125 DEG C, and in the time of-10～+ 85 DEG C, accuracy of temperature control is ± 0.5 DEG C, programmable best result distinguishes that temperature is 0.0625 DEG C.
Described well heater 2 is Peltier, and as shown in Figure 4, sink and form the cavity cooperatively interacting with amplified reaction chip in middle part.
Described heating radiator 3 as shown in Figure 4, is heat radiator.
Described fluorescence excitation unit 4, as shown in Figure 5, comprises light source 41, lens 42 and optical filter 43.Described light source 41 adopts the long-term durability luminous diode of miniwatt as light source.Described lens 42, adopt short Jiao poly-apart from focus lamp, and its focal length is 8mm.Described optical filter 43 is selected according to fluorescence indicator, selects calcein as fluorescence indicator, and the optical filter 43 that excites unit is band pass filter, wavelengths centered 470nm, bandwidth 10nm.Described diode sends fluorescent exciting, focuses on through too short burnt condenser lens 42, and mating plate 43 after filtration, projects on the sample in the reacting hole 16 of amplified reaction chip, launches fluorescence.
Described fluorescence reception unit 5, as shown in Figure 5, comprises apart from condenser lens 52, photoelectric tube 51 and optical filter 53.Described lens 52, its focal length is 8mm.Described photoelectric tube 51, for the fluorescence signal of collecting through lens and optical filter 53 is converted to electric signal, is selected the high sensitivity PIN photodiode BPW21 of pA level.Described optical filter 53 is selected according to fluorescence indicator, selects calcein as fluorescence indicator, and the optical filter 53 of receiving element is high permeability band pass filter, wavelengths centered 535nm, bandwidth 20nm.
Described amplified reaction chip 1 is entrenched on described well heater 2, between described amplified reaction chip 1 and described well heater 2, be provided with temperature controller 6, described well heater 2 has heating radiator 3, fluorescence excitation unit 4 is vertically installed in described amplified reaction chip 1 reacting hole 16 tops, described fluorescence reception unit 5 is arranged at amplified reaction chip 1 reacting hole 16 tops and is angle α with described fluorescence excitation unit 4, and angle α is 45 degree.Described fluorescent emission unit and fluorescence reception unit 5, the focal length that the distance of its lens distance reacting hole 16 is selected lens.Described well heater 2 is placed on circular turntable 7, can rotate around central shaft, drives chip to be rotated around central shaft, and corresponding reacting hole 16 is detected in fluorescent emission unit and 5 belows, fluorescence reception unit.
Embodiment 2
A kind of pathogen detection instrument, as shown in Figure 6, comprises transparent amplified reaction chip 1, temperature controller 6, well heater 2, heating radiator 3, fluorescence excitation unit 4 and fluorescence reception unit 5.
Described amplified reaction chip 1 as shown in Figure 7, is organic glass material.Described amplified reaction chip 1 also can comprise from top to bottom place two-layer, upper strata 18 is for being provided with the dimethyl silicone polymer layer of sample holes 15, pipeline and sample outlet hole 17, lower floor is the glassy layer that is provided with reacting hole 16, dimethyl silicone polymer layer covers on glassy layer, and each reacting hole 16 is communicated with corresponding sample holes 15 and sample outlet hole 17 by supratectal passage.Response sample enters the reacting hole 16 on glassy layer by the sample holes 15 of entering on dimethyl silicone polymer layer, in the interior acceptance detection of reacting hole 16, after having detected, discharges from sample outlet hole 17 by pipeline.
Described temperature controller 6, as shown in Figure 8, is temperature sensor DS18B20, temperature-measuring range-55 DEG C～+ 125 DEG C, and in the time of-10～+ 85 DEG C, accuracy of temperature control is ± 0.5 DEG C, programmable best result distinguishes that temperature is 0.0625 DEG C.
Described well heater 2, as shown in Figure 8, is Electric radiant Heating Film, is placed in the below of reaction chip, and physical dimension is consistent with chip.
Described heating radiator 3 as shown in Figure 8, is heat radiator.
Described fluorescence excitation unit 4, as shown in Figure 9, comprises light source 41, lens 42 and optical filter 43.Described light source 41 adopts the long-term durability luminous diode of miniwatt as light source 41, and diode is only automatically lighted or manually touched button and light by program in the time detecting, and saves the energy, can also extend the life-span of diode.Described lens 42, adopt short focal length focus lamp, and its focal length is 3mm.Described optical filter 43 is selected according to fluorescence indicator, selects calcein as fluorescence indicator, excites the optical filter 43 of unit to select band pass filter, wavelengths centered 470nm, bandwidth 10nm.Described diode sends fluorescent exciting, focuses on through too short burnt condenser lens 42, and mating plate 43 after filtration, projects on the sample in the reacting hole 16 of amplified reaction chip 1.
Described fluorescence reception unit 5, as shown in Figure 9, comprises apart from condenser lens 52 and photoelectric tube 51.Described lens, its focal length is 3mm.Described photoelectric tube, for the fluorescence signal of collecting through lens is converted to electric signal, is selected the high sensitivity PIN photodiode BPW21 of pA level.
Described amplified reaction chip 1 is entrenched on described well heater 2, between described amplified reaction chip 1 and described well heater 2, be provided with temperature controller 6, described well heater 2 has heating radiator 3, fluorescence excitation unit 4 is vertically installed in described amplified reaction chip 1 reacting hole top, described fluorescence reception unit 5 is arranged at amplified reaction chip 1 reacting hole top and is angle α with described fluorescence excitation unit 4, and angle α is 10 degree.Described fluorescent emission unit and fluorescence reception unit 5, the focal length that the distance of its lens distance reacting hole is selected lens.Fluorescence excitation unit 4 and fluorescence reception unit 5 are arranged on circular turntable 7, can rotate around central shaft, make fluorescence excitation unit 4 successively on chip the top in differential responses hole detect.
Embodiment 3
A kind of pathogen detection instrument, as shown in figure 10, comprises transparent amplified reaction chip 1, temperature controller 6, well heater 2, heating radiator 3, fluorescence excitation unit 4 and fluorescence reception unit 5.
Described amplified reaction chip 1 as shown in figure 11, is quartzy material.Described amplified reaction chip 1 is a kind of porous plate, comprise two-layer up and down, lower floor 19 is for being provided with the transparent glass layer of reacting hole 16, upper strata 18 is for there being the overlayer of interface channel, sample holes 15 and sample outlet hole 17, it is the array reacting hole 16 that 2 row 4 are listed as that glassy layer is loaded with, each reacting hole 16 is independent, and each reacting hole 16 is by overlayer, sample holes 15 and the sample outlet hole 17 of correspondence are communicated with it.
Described temperature controller 6, as shown in figure 12, is temperature sensor DS18B20, temperature-measuring range-55 DEG C～+ 125 DEG C, and in the time of-10～+ 85 DEG C, accuracy of temperature control is ± 0.5 DEG C, programmable best result distinguishes that temperature is 0.0625 DEG C.
Described well heater 2, as shown in figure 12, is the brace table that electric-heating coatings covers, and is placed in the below of amplified reaction chip 1, and physical dimension is consistent with amplified reaction chip 1 shape.
Described heating radiator 3 as shown in figure 12, is fan.
Described fluorescence excitation unit 4, as shown in figure 13, comprises light source 41 and lens 42.Described light source 41 adopts the long-term durability luminous diode of miniwatt as light source.Described lens 42, adopt short focal length focus lamp, and its focal length is 15mm.Described diode sends fluorescent exciting, focuses on through too short burnt condenser lens, and mating plate after filtration if desired, projects on the sample in the reacting hole 16 of amplified reaction chip 1.
Described fluorescence reception unit 5, as shown in figure 13, comprises apart from condenser lens 52, photoelectric tube 51 and optical filter 53.Described lens 52, its focal length is 15mm.Described photoelectric tube 51, for the fluorescence signal of collecting through lens 52 and optical filter 53 is converted to electric signal, is selected the high sensitivity PIN photodiode BPW21 of pA level.Described optical filter 53 is selected according to fluorescence indicator, selects calcein as fluorescence indicator, and the optical filter of receiving element is high permeability band pass filter, wavelengths centered 535nm, bandwidth 20nm.
Described amplified reaction chip 1 is entrenched on described well heater 2, between described amplified reaction chip 1 and described well heater 2, be provided with temperature controller, described well heater 2 has heating radiator 3, fluorescence excitation unit 4 is vertically installed in described amplified reaction chip 1 reacting hole 16 tops, described fluorescence reception unit 5 is arranged at amplified reaction chip 1 reacting hole 16 tops and is angle α with described fluorescence excitation unit 4, and angle α is 80 degree.Described fluorescence excitation unit 4 and fluorescence reception unit 5, the focal length that the distance of its lens distance reacting hole 16 is selected lens.Described heating radiator 3 is placed on translation stage 8, can be along carrying out horizontal reciprocating movement, and reacting hole 16 is detected in fluorescence excitation unit 4 and 5 belows, fluorescence reception unit successively.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present invention; not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (8)
1. a pathogen detection instrument, it is characterized in that, comprise transparent amplified reaction chip (1), temperature controller (6), well heater (2), heating radiator (3), fluorescence excitation unit (4) and fluorescence reception unit (5), described amplified reaction chip (1) is provided with reacting hole (16), described amplified reaction chip (1) is entrenched on described well heater (2), between described amplified reaction chip (1) and described well heater (2), be provided with temperature control device (6), described well heater (2) has heating radiator (3), fluorescence excitation unit (4) is vertically installed in described amplified reaction chip reacting hole (16) top, described fluorescence reception unit (5) is arranged at amplified reaction chip reacting hole (16) top and is angle α with described fluorescence excitation unit (4), angle α at 10 degree between 80 degree.
2. pathogen detection instrument as claimed in claim 1, it is characterized in that, on described amplified reaction chip (1), there are multiple reacting holes (16), described fluorescence excitation unit (4) and fluorescence reception unit (5) are arranged on one of them reacting hole (16) top, described amplified reaction chip (1) can be movable relatively with described fluorescence excitation unit (4) and fluorescence reception unit (5), makes reacting hole (16) successively in described fluorescence excitation unit (4) and fluorescence reception unit (5) below.
3. pathogen detection instrument as claimed in claim 1 or 2, is characterized in that, the material of described amplified reaction chip (1) is glass.
4. pathogen detection instrument as claimed in claim 1 or 2, is characterized in that, described well heater (2) is Peltier.
5. pathogen detection instrument as claimed in claim 1 or 2, is characterized in that, described heating radiator (3) is passive radiator.
6. pathogen detection instrument as claimed in claim 1 or 2, is characterized in that, in described fluorescence excitation unit (4) and fluorescence reception unit (5), at least one comprises optical filter.
7. pathogen detection instrument as claimed in claim 6, it is characterized in that, described fluorescence excitation unit (4) comprises light source (41) and lens (42), described light source sends fluorescent exciting, through lens focus, project on the sample in the reacting hole (16) of amplified reaction chip (1).
8. pathogen detection instrument as claimed in claim 6, it is characterized in that, described fluorescence reception unit (5) comprises lens (52) and photoelectric tube (51), sample in the reacting hole (16) of amplified reaction chip (1) is through excitation, launch fluorescence, after lens (52) focus on, caught by photoelectric tube (51).
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| CN111426666A (en) * | 2020-02-27 | 2020-07-17 | 中国科学院上海技术物理研究所 | High-throughput automatic detector for simultaneously detecting multiple kinds of viruses |
| WO2023012511A1 (en) * | 2021-08-04 | 2023-02-09 | Centro De Investigación Y De Estudios Avanzados Del Instituto Politécnico Nacional | PORTABLE HYBRID OPTO-THERMAL CYCLER FOR RT-qPCR USING A BUBBLE-FREE MICROFLUIDIC DEVICE |
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