CN209810206U - Centrifugal micro-fluidic chip clamping device - Google Patents
Centrifugal micro-fluidic chip clamping device Download PDFInfo
- Publication number
- CN209810206U CN209810206U CN201920164676.2U CN201920164676U CN209810206U CN 209810206 U CN209810206 U CN 209810206U CN 201920164676 U CN201920164676 U CN 201920164676U CN 209810206 U CN209810206 U CN 209810206U
- Authority
- CN
- China
- Prior art keywords
- centrifugal
- micro
- microfluidic chip
- channel
- pressing hand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model relates to a centrifugal micro-fluidic chip clamping device. Specifically, the utility model provides a centrifugal microfluidic chip clamping device, which comprises a centrifugal microfluidic chip positioning part and a transmission part; the centrifugal micro-fluidic chip positioning part comprises a centrifugal micro-fluidic chip positioning tray, a centrifugal pressing hand shaft and a centrifugal pressing hand, and the centrifugal pressing hand is provided with a through hole; the periphery of the centrifugal microfluidic chip positioning tray is provided with a suspension through hole, the centrifugal pressing hand shaft penetrates through the through hole of the centrifugal pressing hand, and two ends of the centrifugal pressing hand shaft penetrate through the suspension through hole; in the non-centrifugal state, the centrifugal pressing hand is in a suspension state, and in the centrifugal state, the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft with the lower end outwards and the upper end inwards and downwards. The utility model discloses centrifugal micro-fluidic chip clamping device can guarantee that centrifugal micro-fluidic chip can not take place to drop and realize fast that centrifugal micro-fluidic chip gel is filled in centrifugal process.
Description
Technical Field
The utility model relates to a micro-fluidic chip clamping device field specifically relates to a centrifugal micro-fluidic chip clamping device.
Background
The existing centrifugal microfluidic chip assembly method is as follows: a rotating shaft mounting hole is formed in the center of the microfluidic chip and is assembled on the rotating shaft through a spring buckle; this type of assembly has the following disadvantages: the spring buckle has certain buckle force, the operation of placing and taking out the microfluidic chip is relatively difficult, and a reagent or a sample is easy to spill when the microfluidic chip is taken out; in addition, the center of the microfluidic chip is provided with a rotating shaft mounting hole, which occupies the original reagent feeding port and reagent cavity, so that the diameter of the microfluidic chip is relatively increased, and particularly, when DNA and protein gel electrophoresis experiments are carried out, a good separation effect is required to be obtained, a glue running channel with enough length must be arranged, the diameter of the chip must be increased, the diameter of the microfluidic chip is large, and the micro-fluidic chip is not beneficial to man-machine operation and the increase of the manufacturing cost of the microfluidic chip.
Therefore, the clamping device for the centrifugal microfluidic chip is required to be developed, wherein the clamping device is convenient to mount, the length and the volume of the channel of the centrifugal microfluidic chip are increased, and the centrifugal microfluidic chip is prevented from falling off in the centrifugal process.
SUMMERY OF THE UTILITY MODEL
A first object of the utility model is to provide a centrifugal micro-fluidic chip's clamping device that the installation is convenient, increase centrifugal micro-fluidic chip passageway length and volume and guarantee that centrifugal micro-fluidic chip can not take place to drop in centrifugal process.
The utility model discloses a another aim at improves gel channel preparation efficiency, improves gel electrophoresis experimental efficiency's centrifugal micro-fluidic chip.
The utility model provides a centrifugal micro-fluidic chip clamping device in a first aspect, which comprises a positioning part and a transmission part of the centrifugal micro-fluidic chip;
the centrifugal micro-fluidic chip positioning part comprises a centrifugal micro-fluidic chip positioning tray 1.1, a centrifugal pressing hand shaft 1.2 and a centrifugal pressing hand 1.3, and the centrifugal pressing hand is provided with a through hole 1.4;
the periphery of the centrifugal microfluidic chip positioning tray is provided with a suspension through hole 1.5, the centrifugal pressing hand shaft penetrates through the through hole of the centrifugal pressing hand, two ends of the centrifugal pressing hand shaft penetrate through the suspension through hole, and the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft;
in the non-centrifugal state, the centrifugal pressing hand is in a suspended state, and in the centrifugal state, the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft with the lower end outwards and the upper end inwards and downwards;
the centrifugal micro-fluidic chip positioning tray is connected with the transmission part, and the transmission part can drive the centrifugal micro-fluidic chip positioning tray to rotate.
In another preferred example, the chip is centrifugally pressed on the cylindrical shaft of the hand shaft.
In another preferred example, the centrifugal microfluidic chip positioning tray is disc-shaped.
In another preferred embodiment, the number of the suspension through holes is 2-8, and more preferably 3-6.
In another preferred example, the cross-sectional area of the perforation is larger than or equal to the cross-sectional area of the centrifugal presser shaft.
In another preferred example, in the non-centrifugal state, a gap exists between the perforation of the centrifugal pressing hand and the centrifugal pressing hand shaft.
In another preferred embodiment, the height of said gap is 1-4mm in the non-centrifuged state.
In another preferred embodiment, the center of gravity of the centrifugal press hand is located below the perforation when the centrifugal press hand is in an unsynchronized state.
In another preferred example, the through hole is a kidney-shaped through hole.
In another preferred example, the centrifugal pressing hand is a "Z" type centrifugal pressing hand, the "Z" type centrifugal pressing hand comprises a pressing hand head 1.6, a pressing hand body 1.7 and a pressing hand tail 1.8, the pressing hand head and the pressing hand body form an angle of 50-110 degrees, the pressing hand tail and the pressing hand body form an angle of 70-110 degrees, and the angle formed by the pressing hand head and the pressing hand body and the angle formed by the pressing hand tail and the pressing hand body face opposite directions.
In another preferred example, the hand pressing head and the hand pressing body form an angle towards the micro-fluidic chip positioning tray.
In another preferred example, the centrifugal microfluidic chip positioning tray is further provided with a pressing hole 1.9 for pressing the centrifugal pressing hand shaft.
In another preferred example, the pressing hole is a pressing threaded hole.
In another preferred example, the pressing screw hole is screwed by a screw for pressing the chip centrifugal pressing hand shaft.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with an installation groove, and the centrifugal pressing hand shaft is arranged on the installation groove.
In another preferred embodiment, the transmission part comprises a motor 1.10 and a rotating shaft 1.11.
In another preferred example, the rotating shaft is connected with the motor, and the microfluidic chip positioning tray is connected with the rotating shaft.
In another preferred embodiment, the motor is provided with a motor shaft, and the rotating shaft is connected to the motor shaft of the motor through a diaphragm coupling.
In another preferred example, the motor is a servo motor.
In another preferred example, the center of the bottom of the centrifugal microfluidic chip positioning tray is connected with the transmission part.
In another preferred example, the centrifugal microfluidic chip positioning tray is provided with positioning pins 1.12.
In another preferred example, the number of the positioning pins is 2-6.
In another preferred example, the centrifugal microfluidic chip positioning tray is provided with a groove on the upper surface thereof for placing the microfluidic chip.
In another preferred example, the slot position is a circular slot position.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is provided with a plurality of strip-shaped holes.
In a second aspect of the present invention, a centrifugal microfluidic chip is provided, which comprises a channel disc 2.1;
the channel disc is provided with a plurality of liquid storage cavities 2.2, a circular reagent cavity is arranged in the central area of the channel disc, each liquid storage cavity is communicated with the circular reagent cavity 2.3, each liquid storage cavity is connected with a main micro-channel 2.4, the tail end of the main micro-channel far away from the liquid storage cavity is communicated with a first exhaust cavity through a main pore channel 2.5, one end of the main micro-channel close to the main pore channel is provided with a left micro-channel 2.6 and a right micro-channel 2.7, and the tail ends of the left micro-channel and the right micro-channel are respectively communicated with a second exhaust cavity and a third exhaust cavity through a left pore channel 2.8 and a right pore channel 2.9;
the channel disc is provided with a liquid filling port 2.10, the liquid filling port is communicated with the liquid storage cavity or the circular reagent cavity, and the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with an exhaust hole 2.11.
In another preferred embodiment, the liquid storage cavity, the circular reagent cavity, the main microchannel, the left microchannel, the right microchannel, the main pore channel, the left pore channel and/or the right pore channel are all conductive.
In another preferred embodiment, the liquid storage cavities are independent from each other.
In another preferred embodiment, the channel disc is a circular disc.
In another preferred embodiment, the ends of the left microchannel and the right microchannel refer to the ends far away from the main microchannel.
In another preferred example, the filling opening is provided with a glue film 2.18.
In another preferred example, the number of the filling openings is 1 or more.
In another preferred embodiment, the number of the liquid filling ports is 2-6, preferably 2-3.
In another preferred embodiment, the first exhaust chamber comprises a first exhaust channel 2.12 and a first excess reagent chamber 2.13.
In another preferred embodiment, the second exhaust chamber comprises a second exhaust channel 2.14 and a second excess reagent chamber 15.
In another preferred embodiment, the third exhaust chamber comprises a third exhaust channel 2.16 and a third excess reagent chamber 2.17.
In another preferred embodiment, the vent hole is arranged on the first excess reagent chamber.
In another preferred embodiment, said vent is provided in said second excess reagent chamber.
In another preferred embodiment, the vent hole is formed on the third excess reagent chamber.
In another preferred embodiment, the main hole is communicated with the first excess reagent cavity through a first exhaust passage.
In another preferred example, the left hole channel is communicated with the second excess reagent cavity through a second exhaust channel.
In another preferred example, the right hole channel is communicated with the third excess reagent cavity through a third exhaust channel.
In another preferred example, the liquid storage cavity, the circular reagent cavity, the main micro-channel, the left micro-channel and/or the right micro-channel are/is arranged close to the lower surface side of the channel disc.
In another preferred embodiment, the first exhaust chamber, the second exhaust chamber and/or the third exhaust chamber are arranged near the upper surface side of the channel plate.
In another preferred example, the sum of the volumes of the circular reagent cavity and each liquid storage cavity is larger than the sum of the volumes of each main micro-channel, each left micro-channel, each right micro-channel, each main pore channel and each main pore channel.
In another preferred embodiment, the main microchannel, the left microchannel and the right microchannel have the same hydrophilicity.
In another preferred embodiment, the number of the liquid storage cavities is 2-60, preferably 6-40, more preferably 8-30, and most preferably 10-30.
In another preferred example, the main microchannel has a hydrophilicity smaller than that of the reservoir chamber.
In another preferred embodiment, each main microchannel has a hydrophilicity less than that of the reservoir chamber to which it is connected.
In another preferred example, the liquid storage cavity is made of hydrophilic materials or the surface of the liquid storage cavity is subjected to hydrophilic treatment.
In another preferred embodiment, the main microchannel is made of hydrophobic material or has hydrophobic surface.
In another preferred embodiment, the liquid storage cavities are uniformly arranged around the circular reagent cavity; and/or
The center of the channel disc is coincident with the center of the circular reagent cavity.
In another preferred embodiment, the main microchannels are distributed along the center of the channel disc in the direction of the periphery of the channel disc.
In another preferred embodiment, the area S1 of the longitudinal section of the main microchannel is 0.002 mm2≤S1≤0.005mm2(ii) a And/or
The area S2 of the longitudinal section of the left micro-channel is 0.003mm2≤S2≤0.007mm2(ii) a And/or
The area S3 of the longitudinal section of the right micro-channel is 0.003mm2≤S3≤0.007mm2。
In another preferred example, the longitudinal section of the left microchannel is rectangular, the length of the left microchannel is 0.10-0.14mm, and the width of the left microchannel is 0.03-0.05 mm.
In another preferred embodiment, the longitudinal section of the main microchannel is rectangular, the length of the main microchannel is 0.06-0.10mm, and the width of the main microchannel is 0.03-0.05 mm.
In another preferred example, the longitudinal section of the right microchannel is rectangular, the length of the right microchannel is 0.10-0.14mm, and the width of the right microchannel is 0.03-0.05 mm.
In another preferred embodiment, the main microchannel, the left microchannel and/or the right microchannel is a straight channel.
In another preferred embodiment, the longitudinal section of the main microchannel, the left microchannel and/or the right microchannel is circular, rectangular or square.
In another preferred embodiment, the longitudinal section of the main microchannel, the left microchannel and/or the right microchannel is rectangular.
In another preferred embodiment, the main microchannel, the left microchannel and/or the right microchannel is a straight channel.
In another preferred example, the areas of the longitudinal sections of the left micro-channel and the right micro-channel are equal.
In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.8 to 2 times, preferably 1.0 to 2 times, more preferably 1.2 to 1.8 times, still more preferably 1.3 to 1.7 times, and most preferably 1.4 to 1.6 times the area S1 of the longitudinal section of the main microchannel.
In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.8 to 2 times, preferably 1.0 to 2.0 times, more preferably 1.2 to 1.8 times, still more preferably 1.3 to 1.7 times, most preferably 1.4 to 1.6 times the area S1 of the longitudinal section of the main microchannel.
In another preferred embodiment, the included angle α 1 between the left microchannel and the main microchannel is 56 ± 0.5 °; and/or
The included angle alpha 2 between the right micro-channel and the main micro-channel is 56 +/-0.5 degrees.
In another preferred embodiment, the included angle α 1 between the left microchannel and the main microchannel is equal to the included angle α 2 between the right microchannel and the main microchannel.
In another preferred example, each liquid storage cavity is a long hole type liquid storage cavity, and the dimensional tolerance of the long hole type liquid storage cavity is 0.015-0.025mm, and the depth is 4.8-5.2 mm.
In another preferred example, the centrifugal microfluidic chip is made of a transparent material.
In another preferred embodiment, the circular reagent chamber is raised on the channel plate.
In another preferred embodiment, the channel disc is further provided with positioning holes 2.19.
The utility model discloses the third aspect provides a gel electrophoresis equipment, equipment include the utility model discloses the first aspect centrifugal micro-fluidic chip clamping device with the utility model discloses the second aspect centrifugal micro-fluidic chip.
In another preferred example, the centrifugal microfluidic chip is mounted on the centrifugal microfluidic chip positioning tray.
It is understood that within the scope of the present invention, the above-mentioned technical features of the present invention and those specifically described below (e.g. in the examples) can be combined with each other to constitute new or preferred technical solutions. Not to be reiterated herein, but to the extent of space.
Drawings
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted; the same or similar reference numerals correspond to the same or similar parts; the terms describing positional relationships in the drawings are for illustrative purposes only and are not to be construed as limiting the patent.
Fig. 1 is a schematic structural diagram of a centrifugal microfluidic chip positioning tray.
Fig. 2 is a schematic structural view of a centrifugal hand presser.
Fig. 3 is an overall schematic diagram of a centrifugal microfluidic chip clamping device provided with a centrifugal microfluidic chip.
Fig. 4 is a side sectional view of the centrifugal microfluidic chip clamping device with the centrifugal microfluidic chip mounted thereon.
Fig. 5 shows a state in which the hand is pressed by centrifugation in an unsrifugated state.
Fig. 6 shows a state in which the hand is pressed centrifugally in the centrifugal state.
Fig. 7 is an overall schematic diagram of a channel disk of a centrifugal microfluidic chip.
Fig. 8 is a schematic structural diagram of a channel plate cross-cut segment of a centrifugal microfluidic chip, wherein a is an upper cross-cut segment, B is a middle cross-cut segment, and C is a lower cross-cut segment.
Fig. 9 is a schematic view of the lower surface of the middle transverse segment of the channel disk.
Fig. 10 is a structural view of a region D of the lower surface of the middle traverse section of the channel disk.
Wherein the numbering in each drawing is as follows:
1.1 is a centrifugal micro-fluidic chip positioning tray, 1.2 is a centrifugal hand pressing shaft, 1.3 is a centrifugal hand pressing, 1.4 is a through hole, 1.5 is a suspension through hole, 1.6 is a hand pressing head, 1.7 is a hand pressing body, 1.8 is a hand pressing tail, 1.9 is a pressing hole, 1.10 is a motor, 1.11 is a rotating shaft and 1.12 is a positioning pin;
2.1 is a channel disc, 2.2 is a liquid storage cavity, 2.3 is a circular reagent cavity, 2.4 is a main micro-channel, 2.5 is a main pore channel, 2.6 is a left micro-channel, 2.7 is a right micro-channel, 2.8 is a left pore channel, 2.9 is a right pore channel, 2.10 is a liquid adding port, 2.11 is an exhaust hole, 2.12 is a first exhaust channel, 2.13 is a first excess reagent cavity, 2.14 is a second exhaust channel, 2.15 is a second excess reagent cavity, 2.16 is a third exhaust channel, 2.17 is a third excess reagent cavity, 2.18 is a glue film and 2.19 is a positioning hole.
Detailed Description
The inventor of the invention develops a centrifugal microfluidic chip clamping device and a centrifugal microfluidic chip for the first time through extensive and intensive research.
In the centrifugal microfluidic chip clamping device, the periphery of the centrifugal microfluidic chip positioning tray is provided with a hanging through hole, the centrifugal pressing hand shaft passes through the through hole of the centrifugal pressing hand, two ends of the centrifugal pressing hand shaft pass through the hanging through hole, the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft, the centrifugal pressing hand is in a hanging state in an unsynchronized state, and the centrifugal pressing hand can rotate outwards at the lower end and inwards at the upper end around the centrifugal pressing hand shaft in a centrifugal state. The centrifugal force of the centrifugal microfluidic chip clamping device is larger, the clamping force of the centrifugal presser to the microfluidic chip is larger, and the centrifugal microfluidic chip can be prevented from falling off in the centrifugal process.
In the centrifugal micro-fluidic chip, the micro-fluidic chip comprises a channel disc, a proper amount of gel is filled into the centrifugal micro-fluidic chip at one time through a feeding port of the channel disc, then the centrifugal micro-fluidic chip is placed in the existing centrifugal device, the gel is filled into the corresponding main micro-channel, left micro-channel and right micro-channel through centrifugation, when the gel appears in the first exhaust cavity, the second exhaust cavity and the third exhaust cavity, the gel is completely filled, and when the gel is filled in the centrifugal device, the air in the main micro-channel, the left micro-channel and the right micro-channel is exhausted through the exhaust holes of the exhaust cavities under the action of centrifugation, so that the gel filled in the main micro-channel, the left micro-channel and the right micro-channel is prevented from wiping out the impurity bubbles, and the quality of the gel filling is ensured. The utility model discloses a centrifugal micro-fluidic chip can be fast, accurate realize the packing of multichannel gel, improve experiment (electrophoresis) efficiency.
On this basis, the inventors have completed the present invention.
Term(s) for
As used herein, the terms "comprises," "comprising," "includes," "including," and "including" are used interchangeably and include not only closed-form definitions, but also semi-closed and open-form definitions. In other words, the term includes "consisting of … …", "consisting essentially of … …".
As used herein, the terms "upper", "lower", "horizontal", "left", "right", and the like refer to an orientation or positional relationship based on that shown in the drawings, which is merely for convenience in describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the invention.
As used herein, the term "coupled" is to be construed broadly, as including both fixed and removable couplings or integrally coupled.
As used herein, the term "plurality" means having more than 2.
As used herein, the term "in an un-centrifuged state" is referred to as a horizontal resting state, and refers to a state in which the centrifugal microfluidic chip clamping device is resting on a horizontal plane and the microfluidic chip positioning tray is not rotated.
As used herein, the term "in a centrifugal state" is also referred to as a working body, and refers to a state when the transmission portion drives the micro-fluidic chip positioning tray to rotate.
Centrifugal micro-fluidic chip clamping device
In order to overcome the drawback that current centrifugal micro-fluidic chip assembly quality exists, the utility model provides a centrifugal micro-fluidic chip clamping device.
The centrifugal microfluidic chip clamping device of the present invention is described below with reference to fig. 1-6, it being understood that the drawings are for illustrative purposes only and are not to be construed as limiting the present patent.
Typically, the microfluidic chip clamping device comprises: the device comprises a centrifugal micro-fluidic chip positioning part and a transmission part;
the centrifugal micro-fluidic chip positioning part comprises a centrifugal micro-fluidic chip positioning tray 1.1, a centrifugal pressing hand shaft 1.2 and a centrifugal pressing hand 1.3, and the centrifugal pressing hand is provided with a through hole 1.4;
the periphery of the centrifugal microfluidic chip positioning tray is provided with a suspension through hole 1.5, the centrifugal pressing hand shaft penetrates through the through hole of the centrifugal pressing hand, two ends of the centrifugal pressing hand shaft penetrate through the suspension through hole, and the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft;
in the non-centrifugal state, the centrifugal pressing hand is in a suspended state, and in the centrifugal state, the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft with the lower end outwards and the upper end inwards and downwards;
the centrifugal micro-fluidic chip positioning tray is connected with the transmission part, and the transmission part can drive the centrifugal micro-fluidic chip positioning tray to rotate.
In the present invention, it should be understood that the centrifugal pressing hand surrounds the centrifugal pressing hand shaft to rotate the lower end outwards and upwards, the rotation of the upper end inwards and downwards refers to the rotation of the upper end of the centrifugal pressing hand towards the direction of the positioning tray of the centrifugal micro-fluidic chip, and the rotation of the lower end away from the direction of the positioning tray of the centrifugal micro-fluidic chip.
In the centrifugal microfluidic chip clamping device of the present invention, the number of the hanging through holes is preferably multiple, such as 2-8 or 3-6.
In a preferred embodiment of the present invention, when the container is not centrifuged, the center of gravity of the centrifugal pressing hand is located below the through hole.
In another preferred example, the cross-sectional area of the perforation of the centrifugal pressing hand is larger than or equal to the cross-sectional area of the centrifugal pressing hand shaft. More preferably, in an unsuguration state, a gap exists between the perforation of the centrifugal pressing hand and the centrifugal pressing hand shaft. Typically, the height of the gap is 1-4mm in the non-centrifuged state.
In another preferred embodiment of the present invention, the centrifugal pressing hand is a "Z" type centrifugal pressing hand, said "Z" type centrifugal pressing hand includes a pressing head, a pressing body and a pressing tail, said pressing head and pressing body form an angle of 50-110 °, said pressing tail and pressing body form an angle of 70-110 °, and said pressing head and pressing body form an angle and said pressing tail and pressing body form an angle in opposite directions.
In another preferred example, the hand pressing head and the hand pressing body form an angle towards the micro-fluidic chip positioning tray.
In another preferred example, the centrifugal microfluidic chip positioning tray is further provided with a pressing hole for pressing the centrifugal pressing hand shaft.
In another preferred example, the pressing hole is a pressing threaded hole.
In another preferred example, the pressing screw hole is screwed by a screw for pressing the chip centrifugal pressing hand shaft.
In another preferred embodiment of the present invention, the centrifugal micro-fluidic chip positioning tray is provided with a positioning pin. The positioning pin is used for positioning the centrifugal flow control chip to prevent confusion. The number of the positioning pins is preferably 2-6.
In another preferred embodiment of the present invention, the centrifugal micro-fluidic chip positioning tray is provided with a plurality of elongated holes. The detection equipment can detect the centrifugal microfluidic chip through the long-strip-shaped hole and the centrifugal microfluidic chip positioning tray.
In another preferred embodiment of the present invention, the transmission part includes a motor and a rotating shaft. Typically, the rotating shaft is connected to the motor, and the centrifugal microfluidic chip positioning tray is connected to the rotating shaft.
In another preferred example, the chip is centrifugally pressed on the cylindrical shaft of the hand shaft.
In another preferred example, the centrifugal microfluidic chip positioning tray is disc-shaped.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with an installation groove, and the centrifugal pressing hand shaft is arranged on the installation groove.
Centrifugal micro-fluidic chip
In order to overcome current gel electrophoresis experiment, need at every turn to the electrophoresis tank pour into gel and can only pour into the shortcoming of an electrophoresis tank into through the manual work at every turn, the utility model develops a can once only pack the centrifugal micro-fluidic chip in a plurality of gel channels with the gel automation through the centrifugation.
The centrifugal microfluidic chip of the present invention is described below with reference to fig. 7-10, it being understood that the drawings are for illustrative purposes only and are not to be construed as limiting the present patent.
Typically, a centrifugal microfluidic chip comprising a channel disc 2.1;
the channel disc is provided with a plurality of liquid storage cavities 2.2, a circular reagent cavity is arranged in the central area of the channel disc, each liquid storage cavity is communicated with the circular reagent cavity 2.3, each liquid storage cavity is connected with a main micro-channel 2.4, the tail end of the main micro-channel far away from the liquid storage cavity is communicated with a first exhaust cavity through a main pore channel 2.5, one end of the main micro-channel close to the main pore channel is provided with a left micro-channel 2.6 and a right micro-channel 2.7, and the tail ends of the left micro-channel and the right micro-channel are respectively communicated with a second exhaust cavity and a third exhaust cavity through a left pore channel 2.8 and a right pore channel 2.9;
the channel disc is provided with a liquid filling port 2.10, the liquid filling port is communicated with the liquid storage cavity or the circular reagent cavity, and the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with an exhaust hole 2.11.
In a preferred embodiment, the channel disc is a circular disc.
In another preferred embodiment, the number of said ports is 1 or more, preferably 2 to 6, more preferably 2 to 3.
When the plurality of filling ports are provided, the gel can be added into the liquid storage cavity and/or the circular reagent cavity from one filling port, and when the gel flows out of the other filling ports, the gel in the liquid storage cavity and/or the circular reagent cavity is indicated to be full.
In another preferred embodiment, the filling opening is provided with an adhesive film 18. The glued membrane can avoid the external pollution to the cavity and the passageway of passageway dish.
Centrifugal micro-fluidic chip, first exhaust chamber, second exhaust chamber and third exhaust chamber are equipped with the exhaust hole respectively and can be smoothly with the air escape in main microchannel, left microchannel, the right microchannel of gel filling process, and can avoid the gel that main microchannel, left microchannel, right microchannel were filled to wipe miscellaneous bubble, guarantee the quality that the gel was filled.
The utility model discloses in, stock solution chamber, circular reagent chamber, main microchannel, left microchannel, right microchannel, main pore, left pore, right pore, first exhaust chamber, second exhaust chamber and/or the position of third exhaust chamber in the passageway dish do not have special restriction, as long as satisfy the utility model discloses a can.
In a preferred embodiment of the present invention, the liquid storage chamber, the circular reagent chamber, the main microchannel, the left microchannel and/or the right microchannel are/is disposed near the lower surface side of the channel plate.
In another preferred embodiment, the first exhaust chamber, the second exhaust chamber and/or the third exhaust chamber are arranged near the upper surface side of the channel plate.
In the present invention, the number of the liquid storage chambers is not particularly limited, and may be designed according to the purpose of experiment, and preferably, the number of the liquid storage chambers is 2 to 60, preferably 6 to 40, more preferably 8 to 30, and most preferably 10 to 30.
In a preferred embodiment of the present invention, the main microchannel has a hydrophilicity lower than that of the liquid storage chamber. Typically, each main microchannel is less hydrophilic than the reservoir to which it is connected. The hydrophilicity of the main micro-channel is smaller than that of the liquid storage cavity, so that the main micro-channel, the left micro-channel and the right micro-channel can be filled with gel during centrifugation, and the gel simultaneously enters the first exhaust cavity, the second exhaust cavity and the third exhaust cavity from the main pore channel, the left pore channel and the right pore channel. Experimental research shows that if the hydrophilicity of part of the main microchannels is equal to or greater than that of the liquid storage cavities connected with the main microchannels, the gel can not be ensured to be simultaneously filled in all the main microchannels, the left microchannels and the right microchannels during centrifugation, and the part of the main microchannels can enter the first exhaust cavity, the second exhaust cavity and the third exhaust cavity in advance before or during centrifugation, even can be discharged from the exhaust holes to pollute an instrument in advance, so that the gel filling fails,
in another preferred example, the liquid storage cavity is made of hydrophilic materials or the surface of the liquid storage cavity is subjected to hydrophilic treatment. In another preferred embodiment, the main microchannel is made of hydrophobic material or has hydrophobic surface.
In a preferred embodiment of the present invention, the liquid storage chambers are arranged uniformly around the circular reagent chamber. In another preferred example, the center (circle center) of the channel disc coincides with the center (circle center) of the circular reagent cavity. In the present invention, it should be understood that the coincidence of the centers (circle centers) also means that the channel disc and the circular reagent chamber form a concentric circle.
In another preferred embodiment, the main microchannel, the left microchannel and/or the right microchannel is a straight channel.
In another preferred embodiment, the longitudinal section of the main microchannel, the left microchannel and/or the right microchannel is circular, rectangular or square.
In a preferred embodiment of the present invention, the main microchannels are distributed (extended) along the center of the channel plate toward the periphery of the channel plate.
In another preferred embodiment, the area S1 of the longitudinal section of the main microchannel is 0.002 mm2≤S1≤0.005mm2。
In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.003mm2≤S2≤0.007mm2。
In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.003mm2≤S3≤0.007mm2。
In another preferred example, the longitudinal section of the left microchannel is rectangular, the length of the left microchannel is 0.10-0.14mm, and the width of the left microchannel is 0.03-0.05 mm.
In another preferred embodiment, the longitudinal section of the main microchannel is rectangular, the length of the main microchannel is 0.06-0.10mm, and the width of the main microchannel is 0.03-0.05 mm.
In another preferred example, the longitudinal section of the right microchannel is rectangular, the length of the right microchannel is 0.10-0.14mm, and the width of the right microchannel is 0.03-0.05 mm.
In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.8 to 2 times, preferably 1.0 to 2 times, more preferably 1.2 to 1.8 times, still more preferably 1.3 to 1.5 times, and most preferably 1.4 to 1.6 times the area S1 of the cross section of the main microchannel.
In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.8 to 2 times, preferably 1.0 to 2 times, more preferably 1.2 to 1.8 times, still more preferably 1.3 to 1.5 times, and most preferably 1.4 to 1.6 times the area S1 of the longitudinal section of the main microchannel.
Preferably, the included angle α 1 between the left micro-channel and the main micro-channel is equal to the included angle α 2 between the right micro-channel and the main micro-channel.
The inventor finds through a large amount of experimental researches that when the included angle α 1 between the left microchannel and the main microchannel and the included angle α 2 between the right microchannel and the main microchannel are not in the range of 56 ± 0.5 °, gel is discharged from the gas discharge holes of a part of the plurality of first gas discharge chambers, the plurality of second gas discharge chambers and the plurality of third gas discharge chambers when the gel is filled centrifugally (the centrifugal microfluidic chip and the centrifugal device are polluted by the discharged gel from the gas discharge holes and the centrifugation is stopped), and the gel is not filled in the remaining first gas discharge chambers, the plurality of second gas discharge chambers and the plurality of third gas discharge chambers, so that the main microchannel, the left microchannel and the right microchannel are not filled with the gel, and the requirement of filling all the main microchannel, the left microchannel and the right microchannel is not met, and the gel filling is failed.
In another preferred example, each liquid storage cavity is a long hole type liquid storage cavity, and the dimensional tolerance of the long hole type liquid storage cavity is 0.015-0.025mm, and the depth is 4.8-5.2 mm. The gel in the long-hole liquid storage cavity is concentrated, and is a stress point, namely the opening of the main micro-channel can receive larger centrifugal force, so that the gel can enter the main micro-channel more effectively during centrifugation.
In another preferred embodiment, the circular reagent chamber is protruded on the channel plate.
In another preferred embodiment, the channel disc is further provided with positioning holes 2.19. The positioning holes can be combined with positioning pins on the centrifugal microfluidic chip clamping device, so that the position relation of each channel of the centrifugal microfluidic chip is prevented from being confused.
Centrifugal micro-fluidic chip preparation method
Centrifugal micro-fluidic chip's preparation method do not have special restriction, can adopt the method commonly used among the prior art, just can adopt modes such as mechanical cutting, membrane laminating to prepare, for example, membrane, mesolamella (mesolamella is equipped with a plurality of chamber grooves and channel groove etc.) and lower membrane are gone up in the preparation, go up membrane and lower membrane and mesolamella laminating back, the filling opening and the exhaust hole of preparation of punching prepare centrifugal micro-fluidic chip.
Gel electrophoresis apparatus
The utility model also provides a gel electrophoresis equipment, equipment include centrifugal micro-fluidic chip clamping device with centrifugal micro-fluidic chip.
In another preferred example, the centrifugal microfluidic chip is mounted on the centrifugal microfluidic chip positioning tray.
In the gel electrophoresis apparatus, the centrifugal microfluidic chip holder and the centrifugal microfluidic chip are as described above.
The utility model discloses a main advantage includes:
1. centrifugal micro-fluidic chip clamping device adopt centrifugal clamping's centrifugal micro-fluidic chip of mode clamping, centrifugal micro-fluidic chip clamping device's centrifugal force is big more, centrifugal presser bar is big more to centrifugal micro-fluidic chip's the tight dynamics of clamp, can guarantee that centrifugal micro-fluidic chip can not take place to drop at centrifugal process.
2. Centrifugal micro-fluidic chip clamping device in, under the state of not centrifuging, centrifugal pressure hand is in the suspended state, can conveniently place and take out centrifugal micro-fluidic chip, has avoided placing and taking out the phenomenon of centrifugal micro-fluidic chip difficulty in micro-fluidic chip location tray.
3. Centrifugal micro-fluidic chip clamping device in, centrifugal micro-fluidic chip can directly be put and centrifuge at micro-fluidic chip location tray, need not that the extra center at centrifugal micro-fluidic chip opens there is the rotation axis mounting hole to open the micro-fluidic chip hole that has the rotation axis mounting hole with current center and compare, centrifugal micro-fluidic chip that centrifugal micro-fluidic chip clamping device was suitable for when the volume dwindled, can guarantee that centrifugal micro-fluidic chip's channel length can not become the undersize.
4. The utility model discloses an among the centrifugal micro-fluidic chip, only need artifical liquid-transfering gun that uses, the charge door through the passageway dish is once only filled into proper amount gel in toward centrifugal micro-fluidic chip, put centrifugal micro-fluidic chip in current centrifugal device again, fill corresponding main microchannel, left microchannel and right microchannel with the gel through the centrifugation, when observing first exhaust chamber, second exhaust chamber and third exhaust chamber gel appearance, the gel is filled and is finished, through a series of automation mechanized operations, finally accomplish the gel electrophoresis experiment, therefore, the utility model discloses a centrifugal micro-fluidic chip can be quick, the filling of accurate realization multichannel gel, improve experiment (electrophoresis) efficiency.
5. The utility model discloses a centrifugal micro-fluidic chip is when filling gel through the centrifugation, and the air in main microchannel, left microchannel, the right microchannel is discharged through the exhaust hole in exhaust chamber under centrifugal effect, can avoid the gel that main microchannel, left microchannel, right microchannel were filled to wipe miscellaneous bubble, guarantees the quality that gel was filled.
6. The utility model discloses a centrifugal micro-fluidic chip need not extra and puts at the central point and open the rotation axis mounting hole (through-hole) that supplies the centrifugation to open the micro-fluidic chip hole that has the rotation axis mounting hole with current center and compare, to the centrifugal micro-fluidic chip of the same size, centrifugal micro-fluidic chip have longer passageway, accommodation, can reduce the micro-fluidic chip diameter with to a great extent to when DNA and protein gel electrophoresis, have the isolated channel of sufficient length.
The present invention will be further described with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out under conventional conditions or conditions recommended by the manufacturers. Unless otherwise indicated, percentages and parts are by weight.
Example 1
Embodiment 1 provides a centrifugal microfluidic chip clamping device, which includes a centrifugal microfluidic chip positioning portion and a transmission portion;
the centrifugal micro-fluidic chip positioning part comprises a centrifugal micro-fluidic chip positioning tray 1.1, a centrifugal pressing hand shaft 1.2 and a centrifugal pressing hand 1.3, the centrifugal pressing hand is provided with a through hole 1.4, the centrifugal pressing hand is a Z-shaped centrifugal pressing hand, the Z-shaped centrifugal pressing hand comprises a pressing hand head 1.6, a pressing hand body 1.7 and a pressing hand tail 1.8, the pressing hand head and the pressing hand body form an angle of 105 degrees, the pressing hand tail and the pressing hand body form an angle of 95 degrees, the angle formed by the pressing hand head and the pressing hand body and the angle formed by the pressing hand tail and the pressing hand body face opposite directions, and the angle formed by the pressing hand head and the pressing hand body faces the micro-fluidic chip positioning tray;
the periphery of the centrifugal microfluidic chip positioning tray is provided with 1.5 suspension through holes and 2 positioning pins, the centrifugal pressing hand shaft penetrates through the through hole of the centrifugal pressing hand, two ends of the centrifugal pressing hand shaft penetrate through the suspension through holes, and the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft;
in the non-centrifugal state, the centrifugal pressing hand is in a suspended state, and the center of gravity of the centrifugal pressing hand is positioned below the through hole;
in a centrifugal state, the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft with the lower end outwards and the upper end inwards;
the transmission part comprises a motor 1.10 and a rotating shaft 1.11, the rotating shaft is connected with the motor, the microfluidic chip positioning tray is connected with the rotating shaft, and the transmission part can drive the centrifugal microfluidic chip positioning tray to rotate;
the centrifugal micro-fluidic chip positioning tray is further provided with a pressing hole 1.9, a strip-shaped hole and a mounting groove, the pressing hole is used for pressing a centrifugal pressing hand shaft, the centrifugal pressing hand shaft is arranged on the mounting groove, and the detection equipment can detect the centrifugal micro-fluidic chip through the strip-shaped hole penetrating through the centrifugal micro-fluidic chip positioning tray.
Fig. 1 and 2 are respectively a schematic structural diagram of a centrifugal microfluidic chip positioning tray and a centrifugal pressing hand, fig. 3 and 4 are schematic structural diagrams of a centrifugal microfluidic chip clamping device provided with a centrifugal microfluidic chip, and fig. 5 and 6 are respectively a state of the centrifugal pressing hand in an un-centrifuged state and a centrifuged state.
The working principle of the microfluidic chip clamping device in the embodiment 1 mainly comprises the following steps:
in the non-centrifugal state, the centrifugal pressure hand is in a suspension state (as shown in fig. 5), the centrifugal microfluidic chip is conveniently placed on the centrifugal microfluidic chip positioning tray, and the positioning pin can be combined with the positioning hole of the centrifugal microfluidic chip positioning tray and used for positioning the centrifugal microfluidic chip to prevent confusion; when the motor drives the centrifugal microfluidic chip positioning tray to rotate at a high speed through the rotating shaft, under the action of centrifugal force, the centrifugal pressing hand can rotate outwards at the lower end and downwards at the upper end around the centrifugal pressing hand shaft (as shown in figure 6), so that the centrifugal pressing hand clamps the centrifugal microfluidic chip and generates pressure on the centrifugal microfluidic chip, the centrifugal microfluidic chip is prevented from falling off from the microfluidic chip positioning tray in the centrifugal process, the higher the rotating speed of the centrifugal microfluidic chip positioning tray is, the higher the centrifugal force is, the stronger the centrifugal pressing hand rotates, the higher the pressure generated on the centrifugal microfluidic chip is, and the centrifugal microfluidic chip is prevented from falling off due to high-speed centrifugation.
Example 2
Embodiment 2 provides a centrifugal microfluidic chip, which is schematically shown in fig. 7-10, and includes a circular channel disc 2.1;
the channel disc is provided with a plurality of liquid storage cavities 2.2, a circular reagent cavity is arranged in the central area of the channel disc, each liquid storage cavity is communicated with the circular reagent cavity 2.3, the liquid storage cavities are uniformly arranged around the circular reagent cavity, the circular reagent cavity is protruded on the channel disc, the center of the channel disc is superposed with the center of the circular reagent cavity, each liquid storage cavity is connected with a main micro-channel 2.4, the main micro-channels are distributed along the center of the channel disc to the periphery of the channel disc, the tail end of each main micro-channel far away from the liquid storage cavity is communicated with the first exhaust cavity through a main pore channel 2.5, one end of each main micro-channel close to the main pore channel is provided with a left micro-channel 2.6 and a right micro-channel 2.7, and the tail ends of the left micro-channel and the right micro-channel are respectively communicated with the second exhaust cavity and the third exhaust cavity through a left pore channel 2.8 and a right pore channel;
the channel disc is provided with 2 liquid adding ports 2.10, the liquid adding ports are communicated with the liquid storage cavity, and the liquid adding ports are provided with adhesive films 2.18;
the first exhaust cavity comprises a first exhaust channel 2.12 and a first excess reagent cavity 2.13, the second exhaust cavity comprises a second exhaust channel 2.14 and a second excess reagent cavity 15, the third exhaust cavity comprises a third exhaust channel 2.16 and a third excess reagent cavity 2.17, and the first excess reagent cavity, the second excess reagent cavity and the third excess reagent cavity are respectively provided with an exhaust hole 2.11; the main pore passage is communicated with the first excess reagent cavity through a first exhaust passage, the left pore passage is communicated with the second excess reagent cavity through a second exhaust passage, and the right pore passage is communicated with the third excess reagent cavity through a third exhaust passage;
the hydrophilicity of each main microchannel is less than that of the liquid storage cavity connected with the main microchannel, and the hydrophilicity of the main microchannel, the hydrophilicity of the left microchannel and the hydrophilicity of the right microchannel are the same;
the main microchannel, the left microchannel and/or the right microchannel are straight channels, the longitudinal section of the left microchannel is rectangular, the length of the left microchannel is 0.12mm, the width of the left microchannel is 0.04mm, the longitudinal section of the main microchannel is rectangular, the length of the main microchannel is 0.08mm, the width of the main microchannel is 0.4mm, the longitudinal section of the right microchannel is rectangular, the length of the right microchannel is 0.12mm, and the width of the right microchannel is 0.04 mm;
the included angle alpha 1 between the left micro-channel and the main micro-channel is 56 degrees, and the included angle alpha 2 between the right micro-channel and the main micro-channel is 56 degrees;
the channel disc is also provided with a positioning hole 2.19.
Example 2 centrifugal microfluidic chip gel filling procedure
Adding gel through a liquid adding port of a channel disc, placing the centrifugal micro-fluidic chip in a centrifugal device after the gel is filled in each liquid storage cavity and each circular reagent cavity, centrifuging, filling the gel in the liquid storage cavities and the circular reagent cavities into the corresponding main micro-channel, the left micro-channel and the right micro-channel, almost simultaneously entering the first exhaust cavity, the second exhaust cavity and the third exhaust cavity through the main pore channel, the left pore channel and the right pore channel after the gel is filled in the main micro-channel, the left micro-channel and the right micro-channel, namely when the gel appears in the first exhaust cavity, the second exhaust cavity and the third exhaust cavity, indicating that the main micro-channel, the left micro-channel and the right micro-channel are filled with the gel, stopping centrifuging, obtaining the micro-fluidic chip with the main micro-channel, the left micro-channel and the right micro-channel filled with the gel, thereby realizing one-time feeding, and realizing the filling of the multi-channel gel quickly and accurately by one, and the gel filled in the main micro-channel, the left micro-channel and the right micro-channel is wiped with impurity bubbles, so that the gel filling quality is ensured.
Example 2 centrifugal microfluidic chip DNA electrophoresis experiment
When the main microchannel, the left microchannel and the right microchannel of the centrifugal microfluidic chip in the embodiment 2 are filled with gel, the left channel, the main channel and the right channel are simultaneously punctured by the electrodes of the instrument; then automatically adding a sample containing DNA to be detected into the left channel by the instrument, then inserting the electrodes of the instrument into the left channel and the right channel, connecting the left channel with negative electricity, and connecting the right channel with positive electricity, wherein the sample can flow through the intersection of the main microchannel, the left microchannel and the right microchannel under the action of Lorentz force because the DNA sample is charged with negative electricity; disconnecting the electrode; and then inserting electrodes at the main channel and the liquid filling port, connecting the positive electrode at the liquid filling port, connecting the negative electrode at the main channel, enabling a sample at the intersection of the left channel, the main channel and the right channel to flow into the main microchannel under the action of Lorentz force, combining the sample with a pre-coated reagent in the main microchannel, generating a specific wavelength under the irradiation of laser with the specific wavelength, receiving the wavelength by an optical sensor, producing a corresponding map under the processing of software, and effectively judging the sample by a tester according to the map.
Comparative example 1
The comparative example 1 provides a centrifugal microfluidic chip, the centrifugal microfluidic chip of the comparative example 1 has a similar structure to the centrifugal microfluidic chip of the example 2, and compared with the centrifugal microfluidic chip of the example 2, the centrifugal microfluidic chip of the comparative example 1 is technically characterized in that: in the centrifugal microfluidic chip of comparative example 1, the hydrophilicity of a part of the main microchannels is equal to or greater than that of the reservoir chambers connected thereto.
In the gel filling process of the centrifugal microfluidic chip of comparative example 1, it cannot be ensured that all the main microchannels, the left microchannels and the right microchannels can be simultaneously filled with gel during centrifugation, and part of the main microchannels (the hydrophilicity of which is equal to or greater than that of the liquid storage chambers connected with the main microchannels) can enter the first air exhaust cavity, the second air exhaust cavity and the third air exhaust cavity in advance before or during centrifugation, and even can be discharged from the air exhaust holes in advance to pollute an instrument, so that the gel filling fails.
Comparative examples 2 and 3
The comparative example 2 provides a centrifugal microfluidic chip, the centrifugal microfluidic chip of the comparative example 2 has a similar structure to the centrifugal microfluidic chip of the example 2, and compared with the centrifugal microfluidic chip of the example 2, the centrifugal microfluidic chip of the comparative example 2 is technically characterized in that: in the centrifugal microfluidic chip of comparative example 2, the angle α 1 between the left microchannel and the main microchannel was 54 °, and the angle α 2 between the right microchannel and the main microchannel was 54 °.
Comparative example 3 provides a centrifugal microfluidic chip, the centrifugal microfluidic chip of comparative example 3 has a similar structure to the centrifugal microfluidic chip of example 2, and compared with the centrifugal microfluidic chip of example 2, the centrifugal microfluidic chip of comparative example 3 is technically characterized by the following differences: in the centrifugal microfluidic chip of comparative example 3, the angle α 1 between the left microchannel and the main microchannel was 58 °, and the angle α 2 between the right microchannel and the main microchannel was 58 °.
In the gel filling process of the centrifugal microfluidic chip of the comparative example 2 and the centrifugal microfluidic chip of the comparative example 3, gel cannot enter the first air exhaust cavity, the second air exhaust cavity and the third air exhaust cavity at the same time, when gel is discharged from the air exhaust holes of a part of the first air exhaust cavity, the second air exhaust cavity and the third air exhaust cavity (the centrifugal microfluidic chip and the centrifugal device are polluted by the discharged gel from the air exhaust holes and the centrifugation is stopped), the gel is not filled in the main microchannel, the left microchannel and the right microchannel because the gel is not filled in the rest of the first air exhaust cavity, the second air exhaust cavity and the third air exhaust cavity, and the gel filling fails.
Example 3
Embodiment 3 provides a gel electrophoresis apparatus comprising the centrifugal microfluidic chip holder prepared in embodiment 1 and the centrifugal microfluidic chip prepared in embodiment 2;
the positioning pins on the centrifugal microfluidic chip positioning tray are combined with the positioning holes of the centrifugal microfluidic chip, so that the centrifugal microfluidic chip is installed on the centrifugal microfluidic chip positioning tray (as shown in fig. 3 or fig. 4), after gel is added through the liquid adding port of the channel tray, when the motor drives the centrifugal microfluidic chip positioning tray to rotate at a high speed through the rotating shaft, under the action of centrifugal force, the main microchannel, the left microchannel and the right microchannel on the centrifugal microfluidic chip are quickly filled with gel at one time, and DNA electrophoresis experiments are carried out.
All documents mentioned in this application are incorporated by reference into this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the appended claims.
Claims (9)
1. A centrifugal microfluidic chip clamping device is characterized by comprising a centrifugal microfluidic chip positioning part and a transmission part;
the centrifugal micro-fluidic chip positioning part comprises a centrifugal micro-fluidic chip positioning tray (1.1), a centrifugal pressing hand shaft (1.2) and a centrifugal pressing hand (1.3), and the centrifugal pressing hand is provided with a through hole (1.4);
the periphery of the centrifugal microfluidic chip positioning tray is provided with a suspension through hole (1.5), the centrifugal pressing hand shaft passes through the through hole of the centrifugal pressing hand, two ends of the centrifugal pressing hand shaft pass through the suspension through hole, and the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft;
in the non-centrifugal state, the centrifugal pressing hand is in a suspended state, and in the centrifugal state, the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft with the lower end outwards and the upper end inwards and downwards;
the centrifugal micro-fluidic chip positioning tray is connected with the transmission part, and the transmission part can drive the centrifugal micro-fluidic chip positioning tray to rotate.
2. The centrifugal microfluidic chip holder according to claim 1, wherein the number of the hanging through holes is 2-8.
3. The microfluidic chip holder of claim 1, wherein the center of gravity of the centrifugal presser is located below the through hole in an unsrifugated state.
4. The centrifugal microfluidic chip clamping device according to claim 1, wherein the centrifugal presser is a "Z" type centrifugal presser, the "Z" type centrifugal presser comprises a presser head (1.6), a presser body (1.7) and a presser tail (1.8), the presser head and the presser body form an angle of 50-110 °, the presser tail and the presser body form an angle of 70-110 °, and the angle formed by the presser head and the presser body and the angle formed by the presser tail and the presser body face opposite directions.
5. The centrifugal microfluidic chip clamping device according to claim 4, wherein the angle formed by the hand pressing head and the hand pressing body is towards the microfluidic chip positioning tray.
6. The centrifugal microfluidic chip clamping device according to claim 1, wherein the centrifugal microfluidic chip positioning tray is further provided with a pressing hole (1.9) for pressing the centrifugal pressing hand shaft.
7. The centrifugal microfluidic chip clamping device according to claim 1, wherein the transmission part comprises a motor (1.10) and a rotating shaft (1.11).
8. The centrifugal microfluidic chip clamping device according to claim 1, wherein the centrifugal microfluidic chip positioning tray is provided with positioning pins (1.12).
9. The centrifugal microfluidic chip clamping device according to claim 1, wherein the centrifugal microfluidic chip positioning tray is provided with a plurality of elongated holes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920164676.2U CN209810206U (en) | 2019-01-30 | 2019-01-30 | Centrifugal micro-fluidic chip clamping device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920164676.2U CN209810206U (en) | 2019-01-30 | 2019-01-30 | Centrifugal micro-fluidic chip clamping device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209810206U true CN209810206U (en) | 2019-12-20 |
Family
ID=68872562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920164676.2U Active CN209810206U (en) | 2019-01-30 | 2019-01-30 | Centrifugal micro-fluidic chip clamping device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209810206U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111495443A (en) * | 2019-01-30 | 2020-08-07 | 上海蓝木化工有限公司 | Centrifugal micro-fluidic chip clamping device |
-
2019
- 2019-01-30 CN CN201920164676.2U patent/CN209810206U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111495443A (en) * | 2019-01-30 | 2020-08-07 | 上海蓝木化工有限公司 | Centrifugal micro-fluidic chip clamping device |
| CN111495443B (en) * | 2019-01-30 | 2024-11-05 | 上海蓝木化工有限公司 | Centrifugal microfluidic chip clamping device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20230256451A1 (en) | Unitary Biochip Providing Sample-in to Results-Out Processing and Methods of Manufacture | |
| CN209810205U (en) | Centrifugal micro-fluidic chip | |
| ES2930133T3 (en) | Biochip with reagent storage | |
| US6269957B1 (en) | Ultrafiltration device and method of forming same | |
| CN113249215B (en) | Multi-chamber sample preparation cartridge | |
| US7927474B2 (en) | Cell electrophysiological sensor | |
| CN209810206U (en) | Centrifugal micro-fluidic chip clamping device | |
| US10272433B2 (en) | Vertical functional reaction vessel | |
| JP2007304016A (en) | Blood separation apparatus | |
| KR20150022785A (en) | An electrophoresis system and a separation and identification method | |
| CN111495442A (en) | Centrifugal micro-fluidic chip | |
| CN111495443B (en) | Centrifugal microfluidic chip clamping device | |
| AU2013204367A1 (en) | Unitary biochip providing sample-in to results-out processing and methods of manufacture | |
| AU2020202163A1 (en) | Unitary biochip providing sample-in to results-out processing and methods of manufacture | |
| US11311881B2 (en) | Fluid handling method, fluid handling device used in same, and fluid handling system | |
| JP2011145236A (en) | Micro-fluid chip, and measuring method of specimen using the same | |
| CN215517453U (en) | Multi-chamber sample preparation cartridge with rotating flow path switching valve | |
| CN103805504B (en) | Membrane type separating chips | |
| CN217856737U (en) | Sample disc for centrifugal machine | |
| CN114181825B (en) | Microfluidic centrifugal extrusion cell transfection system under action of external electric field | |
| CN216663018U (en) | Magnetic bead method DNA draws board | |
| JP4670713B2 (en) | Cell electrophysiological sensor and cell electrophysiological measurement method using the same | |
| JP4821449B2 (en) | Cell electrophysiological sensor and method for measuring cell electrophysiology using the same | |
| TW201940202A (en) | Whole blood filtering devices and methods for manufacturing the same | |
| GB2377990A (en) | Multiwell plate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |