CN111495443B - Centrifugal microfluidic chip clamping device - Google Patents
Centrifugal microfluidic chip clamping device Download PDFInfo
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- CN111495443B CN111495443B CN201910093541.6A CN201910093541A CN111495443B CN 111495443 B CN111495443 B CN 111495443B CN 201910093541 A CN201910093541 A CN 201910093541A CN 111495443 B CN111495443 B CN 111495443B
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- 238000003825 pressing Methods 0.000 claims abstract description 142
- 230000005540 biological transmission Effects 0.000 claims abstract description 17
- 230000005484 gravity Effects 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 description 56
- 239000003153 chemical reaction reagent Substances 0.000 description 53
- 238000003860 storage Methods 0.000 description 42
- 230000000052 comparative effect Effects 0.000 description 18
- 239000011148 porous material Substances 0.000 description 17
- 238000005119 centrifugation Methods 0.000 description 16
- 238000001502 gel electrophoresis Methods 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 230000009471 action Effects 0.000 description 6
- 238000001962 electrophoresis Methods 0.000 description 6
- 239000003292 glue Substances 0.000 description 4
- 230000002209 hydrophobic effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000007547 defect Effects 0.000 description 3
- 238000005429 filling process Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002313 adhesive film Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention relates to a centrifugal microfluidic chip clamping device. Specifically, the invention provides a centrifugal microfluidic chip clamping device, which comprises a centrifugal microfluidic chip positioning part and a transmission part; the centrifugal microfluidic chip positioning part comprises a centrifugal microfluidic chip positioning tray, a centrifugal pressing hand shaft and a centrifugal pressing hand, wherein the centrifugal pressing hand is provided with a perforation; the periphery of the centrifugal microfluidic chip positioning tray is provided with a hanging through hole, a centrifugal hand pressing shaft penetrates through a through hole of the centrifugal hand pressing, and two ends of the centrifugal hand pressing shaft penetrate through the hanging through hole; in the non-centrifugal state, the centrifugal pressing hand is in a hanging state, and in the centrifugal state, the centrifugal pressing hand can rotate outwards and upwards around the centrifugal pressing hand shaft, and inwards and downwards at the upper end. The centrifugal microfluidic chip clamping device can ensure that the centrifugal microfluidic chip cannot fall off in the centrifugal process and can rapidly realize the gel filling of the centrifugal microfluidic chip.
Description
Technical Field
The invention relates to the field of microfluidic chip clamping devices, in particular to a centrifugal microfluidic chip clamping device.
Background
The assembly mode of the existing centrifugal microfluidic chip is as follows: the center of the micro-fluidic chip is provided with a rotating shaft mounting hole, and the micro-fluidic chip is assembled on the rotating shaft through a spring buckle; the assembly mode has the following defects: the spring is buckled with a certain buckling force, so that the operation of placing and taking out the microfluidic chip is relatively difficult, and the reagent or sample is easy to splash when the microfluidic chip is taken out; in addition, the center of the microfluidic chip is provided with a rotating shaft mounting hole, so that the diameter of the microfluidic chip is relatively increased due to occupation of an original reagent inlet and a reagent cavity, particularly when DNA and protein gel electrophoresis experiments are carried out, a gel running channel which is long enough is needed, the diameter of the chip is increased, the diameter of the microfluidic chip is large, and man-machine operation is not facilitated, and the manufacturing cost of the microfluidic chip is increased.
Therefore, there is a need in the art to develop a clamping device for a centrifugal microfluidic chip that is convenient to install, increases the channel length and volume of the centrifugal microfluidic chip, and ensures that the centrifugal microfluidic chip does not fall off during centrifugation.
Disclosure of Invention
The invention aims to provide a clamping device for a centrifugal microfluidic chip, which is convenient to install, can increase the channel length and the volume of the centrifugal microfluidic chip and can ensure that the centrifugal microfluidic chip cannot fall off in the centrifugation process.
The invention further aims to improve the preparation efficiency of the gel channel and improve the centrifugal microfluidic chip of the gel electrophoresis experiment efficiency.
The invention provides a centrifugal microfluidic chip clamping device, which comprises a centrifugal microfluidic chip positioning part and a transmission part;
the centrifugal microfluidic chip positioning part comprises a centrifugal microfluidic chip positioning tray 1.1, a centrifugal pressing hand shaft 1.2 and a centrifugal pressing hand 1.3, wherein the centrifugal pressing hand is provided with a perforation 1.4;
the periphery of the centrifugal microfluidic chip positioning tray is provided with a hanging through hole 1.5, the centrifugal hand pressing shaft penetrates through the through hole of the centrifugal hand pressing shaft, two ends of the centrifugal hand pressing shaft penetrate through the hanging through hole, and the centrifugal hand pressing shaft can rotate around the centrifugal hand pressing shaft;
in the non-centrifugal state, the centrifugal press hand is in a hanging state, and in the centrifugal state, the centrifugal press hand can rotate outwards and upwards around the lower end of the centrifugal press hand shaft, and the upper end of the centrifugal press hand rotates inwards and downwards;
The centrifugal microfluidic chip positioning tray is connected with the transmission part, and the transmission part can drive the centrifugal microfluidic chip positioning tray to rotate.
In another preferred embodiment, the chip is a cylindrical shaft of the centrifugal press hand shaft.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is disc-shaped.
In another preferred embodiment, the number of the suspension through holes is 2-8, more preferably 3-6.
In another preferred embodiment, the cross-sectional area of the perforation is greater than or equal to the cross-sectional area of the centrifugal hand shaft.
In another preferred embodiment, in the non-centrifuged state, a gap exists between the perforation of the centrifugal press handle and the centrifugal press handle shaft.
In another preferred embodiment, the gap has a height of 1-4mm in the non-centrifuged state.
In another preferred embodiment, the center of gravity of the centrifugal press handle is located below the perforation in the non-centrifuged state.
In another preferred embodiment, the through holes are kidney-shaped through holes.
In another preferred example, the centrifugal pressing hand is a "Z" type centrifugal pressing hand, the "Z" type centrifugal pressing hand comprises a pressing hand head 1.6, a pressing hand body 1.7 and a pressing hand tail 1.8, the pressing hand head and the pressing hand body form an angle of 50-110 degrees, the pressing hand tail and the pressing hand body form an angle of 70-110 degrees, and the angle formed by the pressing hand head and the pressing hand body and the angle formed by the pressing hand tail and the pressing hand body face opposite directions.
In another preferred embodiment, the angle formed by the hand pressing head and the hand pressing body faces the micro-fluidic chip positioning tray.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with a pressing hole 1.9 for pressing the centrifugal pressing hand shaft.
In another preferred embodiment, the pressing hole is a pressing threaded hole.
In another preferred embodiment, the centrifugal pressing hand shaft is screwed on the pressing threaded hole through a screw for pressing the chip.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with a mounting groove, and the centrifugal hand pressing shaft is arranged on the mounting groove.
In another preferred embodiment, the transmission part comprises a motor 1.10 and a rotating shaft 1.11.
In another preferred embodiment, the rotating shaft is connected with the motor, and the microfluidic chip positioning tray is connected with the rotating shaft.
In another preferred embodiment, the motor is provided with a motor shaft, and the rotating shaft is connected to the motor shaft of the motor through a diaphragm coupling.
In another preferred embodiment, the motor is a servo motor.
In another preferred embodiment, the bottom center of the centrifugal microfluidic chip positioning tray is connected with the transmission part.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is provided with a positioning pin 1.12.
In another preferred embodiment, the number of the positioning pins is 2-6.
In another preferred embodiment, a groove for placing the microfluidic chip is arranged on the upper surface of the centrifugal microfluidic chip positioning tray.
In another preferred embodiment, the slot is a circular slot.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is provided with a plurality of elongated holes.
In a second aspect of the present invention, a centrifugal microfluidic chip is provided, where the centrifugal microfluidic chip includes a channel plate 2.1;
The channel disc is provided with a plurality of liquid storage cavities 2.2, the central area of the channel disc is provided with a circular reagent cavity, each liquid storage cavity is communicated with the circular reagent cavity 2.3, each liquid storage cavity is connected with a main micro-channel 2.4, the tail end of the main micro-channel far away from the liquid storage cavity is communicated with a first exhaust cavity through a main pore canal 2.5, one end of the main micro-channel close to the main pore canal is provided with a left micro-channel 2.6 and a right micro-channel 2.7, and the tail ends of the left micro-channel and the right micro-channel are respectively communicated with a second exhaust cavity and a third exhaust cavity through a left pore canal 2.8 and a right pore canal 2.9;
The channel plate is provided with a liquid adding port 2.10 which is communicated with the liquid storage cavity or the circular reagent cavity, and the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with an exhaust hole 2.11.
In another preferred embodiment, the reservoir, circular reagent chamber, main microchannel, left microchannel, right microchannel, main channel, left channel and/or right channel are electrically conductive.
In another preferred embodiment, the liquid storage cavities are independent from each other.
In another preferred embodiment, the channel plate is a circular plate.
In another preferred embodiment, the left microchannel and the right microchannel end refer to the ends remote from the main microchannel.
In another preferred embodiment, the liquid filling opening is provided with a glue film 2.18.
In another preferred embodiment, the number of the liquid adding ports is 1 or more.
In another preferred embodiment, the number of the liquid adding ports is 2-6, preferably 2-3.
In another preferred embodiment, the first exhaust chamber comprises a first exhaust channel 2.12 and a first excess reagent chamber 2.13.
In another preferred embodiment, the second exhaust chamber comprises a second exhaust channel 2.14 and a second excess reagent chamber 15.
In another preferred embodiment, the third exhaust chamber comprises a third exhaust channel 2.16 and a third excess reagent chamber 2.17.
In another preferred embodiment, the vent is provided in the first excess reagent chamber.
In another preferred embodiment, said vent is provided in said second excess reagent chamber.
In another preferred embodiment, said vent is provided in said third excess reagent chamber.
In another preferred embodiment, the primary port communicates with the first excess reagent chamber via a first exhaust passage.
In another preferred embodiment, the left port communicates with the second excess reagent chamber through a second exhaust passage.
In another preferred embodiment, the right port communicates with a third excess reagent chamber via a third exhaust passage.
In another preferred embodiment, the reservoir, the circular reagent chamber, the main microchannel, the left microchannel and/or the right microchannel are provided near the lower surface side of the channel tray.
In another preferred embodiment, the first exhaust chamber, the second exhaust chamber and/or the third exhaust chamber are provided near the upper surface side of the passage plate.
In another preferred embodiment, the sum of the volumes of the circular reagent chamber and each reservoir chamber is greater than the sum of the volumes of each main microchannel, each left microchannel, each right microchannel, each main orifice, and each main orifice.
In another preferred embodiment, the hydrophilicity of the main microchannel, the left microchannel and the right microchannel are the same.
In another preferred embodiment, the number of the liquid storage cavities is 2-60, preferably 6-40, more preferably 8-30, and most preferably 10-30.
In another preferred embodiment, the primary microchannel has a hydrophilicity less than the hydrophilicity of the reservoir.
In another preferred embodiment, each of the primary microchannels is less hydrophilic than the reservoir connected thereto.
In another preferred embodiment, the liquid storage cavity is made of hydrophilic material or the surface is subjected to hydrophilic treatment.
In another preferred embodiment, the main micro-channel is a hydrophobic material or the surface is subjected to a hydrophobic treatment.
In another preferred embodiment, the individual reservoirs are uniformly arranged around the circular reagent chamber; and/or
The center of the channel disc coincides with the center of the circular reagent cavity.
In another preferred embodiment, the main micro-channels are distributed along the center of the channel plate in a direction towards the periphery of the channel plate.
In another preferred embodiment, the area S1 of the longitudinal section of the main microchannel is 0.002 mm 2≤S1≤0.005mm2; and/or
The area S2 of the longitudinal section of the left micro-channel is 0.003mm 2≤S2≤0.007mm2; and/or
The area S3 of the longitudinal section of the right micro-channel is 0.003mm 2≤S3≤0.007mm2.
In another preferred embodiment, the longitudinal section of the left micro-channel is rectangular, the length is 0.10-0.14mm, and the width is 0.03-0.05mm.
In another preferred embodiment, the longitudinal section of the main micro-channel is rectangular, and the length is 0.06-0.10mm, and the width is 0.03-0.05mm.
In another preferred embodiment, the right micro-channel has a rectangular longitudinal section, a length of 0.10-0.14mm and a width of 0.03-0.05mm.
In another preferred embodiment, the main, left and/or right micro-channels are straight channels.
In another preferred embodiment, the longitudinal cross-section of the main microchannel, the left microchannel and/or the right microchannel is circular, rectangular or square.
In another preferred embodiment, the longitudinal section of the main microchannel, the left microchannel and/or the right microchannel is rectangular.
In another preferred embodiment, the main, left and/or right micro-channels are straight channels.
In another preferred embodiment, the left and right micro-channels have equal areas in longitudinal cross-section.
In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.8-2 times, preferably 1.0-2 times, more preferably 1.2-1.8 times, still more preferably 1.3-1.7 times, most preferably 1.4-1.6 times the area S1 of the longitudinal section of the main microchannel.
In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.8-2 times, preferably 1.0-2.0 times, more preferably 1.2-1.8 times, still more preferably 1.3-1.7 times, most preferably 1.4-1.6 times the area S1 of the longitudinal section of the main microchannel.
In another preferred embodiment, the included angle α1 between the left microchannel and the main microchannel is 56±0.5°; and/or
The included angle alpha 2 between the right micro-channel and the main micro-channel is 56+/-0.5 degrees.
In another preferred embodiment, the angle α1 between the left microchannel and the main microchannel is equal to the angle α2 between the right microchannel and the main microchannel.
In another preferred example, each liquid storage cavity is a long hole type liquid storage cavity, and the dimensional tolerance of the long hole type liquid storage cavity is 0.015-0.025mm, and the depth is 4.8-5.2mm.
In another preferred embodiment, the centrifugal microfluidic chip is made of a transparent material.
In another preferred embodiment, the circular reagent chambers are raised above the channel tray.
In another preferred embodiment, the channel plate is further provided with positioning holes 2.19.
According to a third aspect of the invention, a gel electrophoresis device is provided, and the device comprises the centrifugal microfluidic chip clamping device according to the first aspect of the invention and the centrifugal microfluidic chip according to the second aspect of the invention.
In another preferred embodiment, the centrifugal microfluidic chip is mounted on the centrifugal microfluidic chip positioning tray.
It is understood that within the scope of the present invention, the above-described technical features of the present invention and technical features specifically described below (e.g., in the examples) may be combined with each other to constitute new or preferred technical solutions. And are limited to a space, and are not described in detail herein.
Drawings
The drawings are for illustrative purposes only and are not to be construed as limiting the present patent; for the purpose of better illustrating the embodiments, certain elements of the drawings may be omitted, enlarged or reduced and do not represent the actual product dimensions; it will be appreciated by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted; the same or similar reference numerals correspond to the same or similar components; the terms describing the positional relationship in the drawings are merely illustrative and should not be construed as limiting the present patent.
Fig. 1 is a schematic structural diagram of a centrifugal microfluidic chip positioning tray.
Fig. 2 is a schematic structural view of a centrifugal press handle.
Fig. 3 is an overall schematic diagram of a centrifugal microfluidic chip clamping device mounted with a centrifugal microfluidic chip.
Fig. 4 is a side sectional view of a centrifugal microfluidic chip mounting apparatus mounted with a centrifugal microfluidic chip.
Fig. 5 shows a state of centrifugal hand pressing in an unfiugued state.
Fig. 6 shows a state of centrifugal hand pressing in the centrifugal state.
Fig. 7 is an overall schematic diagram of a channel plate of a centrifugal microfluidic chip.
Fig. 8 is a schematic structural diagram of a channel plate transversal section of a centrifugal microfluidic chip, wherein a is an upper transversal section, B is a middle transversal section, and C is a lower transversal section.
Fig. 9 is a schematic view of the lower surface structure of the middle cross section of the channel plate.
Fig. 10 is a schematic structural view of the D region of the lower surface of the middle transection section of the channel plate.
Fig. 11 is a schematic view of a slot-type liquid storage cavity.
Wherein, the numbers in each figure are:
1.1 is a centrifugal microfluidic chip positioning tray, 1.2 is a centrifugal hand pressing shaft, 1.3 is a centrifugal hand pressing shaft, 1.4 is a perforation, 1.5 is a hanging through hole, 1.6 is a hand pressing head, 1.7 is a hand pressing body, 1.8 is a hand pressing tail, 1.9 is a pressing hole, 1.10 is a motor, 1.11 is a rotating shaft and 1.12 is a positioning pin;
2.1 is a channel disc, 2.2 is a liquid storage cavity, 2.3 is a circular reagent cavity, 2.4 is a main micro-channel, 2.5 is a main channel, 2.6 is a left micro-channel, 2.7 is a right micro-channel, 2.8 is a left channel, 2.9 is a right channel, 2.10 is a liquid filling port, 2.11 is an exhaust hole, 2.12 is a first exhaust channel, 2.13 is a first excess reagent cavity, 2.14 is a second exhaust channel, 2.15 is a second excess reagent cavity, 2.16 is a third exhaust channel, 2.17 is a third excess reagent cavity, 2.18 is a glue film and 2.19 is a positioning hole.
Detailed Description
The inventor has developed a centrifugal microfluidic chip clamping device and a centrifugal microfluidic chip for the first time through extensive and intensive research.
In the centrifugal microfluidic chip clamping device, a hanging through hole is formed in the periphery of the centrifugal microfluidic chip positioning tray, the centrifugal pressing hand shaft penetrates through the through hole of the centrifugal pressing hand, two ends of the centrifugal pressing hand shaft penetrate through the hanging through hole, the centrifugal pressing hand can rotate around the centrifugal pressing hand shaft, in the non-centrifugal state, the centrifugal pressing hand is in a hanging state, in the centrifugal state, the centrifugal pressing hand can rotate outwards and upwards around the lower end of the centrifugal pressing hand shaft, and the upper end of the centrifugal pressing hand can rotate inwards and downwards. The larger the centrifugal force of the centrifugal microfluidic chip clamping device is, the larger the clamping force of the centrifugal pressing hand on the microfluidic chip is, so that the centrifugal microfluidic chip can be prevented from falling off in the centrifugal process.
In the centrifugal microfluidic chip, the microfluidic chip comprises a channel disc, a proper amount of gel is filled into the centrifugal microfluidic chip through a charging hole of the channel disc at one time, then the centrifugal microfluidic chip is placed in an existing centrifugal device, the gel is filled into a corresponding main micro-channel, a left micro-channel and a right micro-channel through centrifugation, when the gel is observed to appear in a first exhaust cavity, a second exhaust cavity and a third exhaust cavity, the gel is filled, and when the gel is filled through centrifugation, the air in the main micro-channel, the left micro-channel and the right micro-channel is discharged through the exhaust holes of the exhaust cavities under the action of centrifugation, so that the gel impurity cleaning bubbles filled in the main micro-channel, the left micro-channel and the right micro-channel are avoided, and the gel filling quality is ensured. The centrifugal microfluidic chip can rapidly and accurately realize the filling of multichannel gel, and improve the experimental (electrophoresis) efficiency.
Terminology
As used herein, the terms "comprising," "including," and "containing" are used interchangeably, and include not only closed-form definitions, but also semi-closed-form and open-form definitions. In other words, the term includes "consisting of … …", "consisting essentially of … …".
As used herein, the terms "upper," "lower," "horizontal," "left," "right," and the like refer to an orientation or positional relationship based on that shown in the drawings, merely to facilitate description of the invention and to simplify the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the invention.
As used herein, the term "coupled" is to be interpreted broadly, as being either fixedly coupled, detachably coupled, or integrally coupled.
The term "plurality" as used herein means having more than 2.
As used herein, the term "in an unfiugued state" is referred to as a horizontal rest state, and refers to a state in which the centrifugal microfluidic chip clamping device is resting on a horizontal plane and the microfluidic chip positioning tray is not rotated.
As used herein, the term "in a centrifugal state" also refers to a state when the driving part drives the microfluidic chip positioning tray to rotate.
Centrifugal microfluidic chip clamping device
The invention provides a centrifugal microfluidic chip clamping device, which aims to overcome the defects of the conventional centrifugal microfluidic chip assembly device.
The centrifugal microfluidic chip clamping device of the present invention will be described with reference to fig. 1-6, it being understood that the drawings are for illustrative purposes only and are not to be construed as limiting the patent.
Typically, the microfluidic chip clamping device comprises: the device comprises a centrifugal microfluidic chip positioning part and a transmission part;
the centrifugal microfluidic chip positioning part comprises a centrifugal microfluidic chip positioning tray 1.1, a centrifugal pressing hand shaft 1.2 and a centrifugal pressing hand 1.3, wherein the centrifugal pressing hand is provided with a perforation 1.4;
the periphery of the centrifugal microfluidic chip positioning tray is provided with a hanging through hole 1.5, the centrifugal hand pressing shaft penetrates through the through hole of the centrifugal hand pressing shaft, two ends of the centrifugal hand pressing shaft penetrate through the hanging through hole, and the centrifugal hand pressing shaft can rotate around the centrifugal hand pressing shaft;
in the non-centrifugal state, the centrifugal press hand is in a hanging state, and in the centrifugal state, the centrifugal press hand can rotate outwards and upwards around the lower end of the centrifugal press hand shaft, and the upper end of the centrifugal press hand rotates inwards and downwards;
The centrifugal microfluidic chip positioning tray is connected with the transmission part, and the transmission part can drive the centrifugal microfluidic chip positioning tray to rotate.
In the present invention, it should be understood that the centrifugal press handle rotates around the centrifugal press handle shaft with the lower end facing outwards and upwards, and the rotation of the upper end facing inwards and downwards means that the upper end of the centrifugal press handle rotates towards the centrifugal microfluidic chip positioning tray, and the lower end rotates away from the centrifugal microfluidic chip positioning tray.
In the centrifugal microfluidic chip clamping device, the number of the hanging through holes is preferably more, for example, 2-8 or 3-6.
In a preferred embodiment of the present invention, the center of gravity of the centrifugal press handle is located below the perforation in the non-centrifuged state.
In another preferred embodiment, the cross-sectional area of the bore of the centrifugal hand grip is greater than or equal to the cross-section of the centrifugal hand grip shaft. More preferably, in the non-centrifuged state, a gap exists between the perforation of the centrifugal press handle and the centrifugal press handle shaft. Typically, the gap is 1-4mm in height in the non-centrifuged state.
In another preferred embodiment of the present invention, the centrifugal pressing hand is a "Z" type centrifugal pressing hand, the "Z" type centrifugal pressing hand includes a pressing hand head, a pressing hand body and a pressing hand tail, the pressing hand head forms an angle of 50-110 ° with the pressing hand body, the pressing hand tail forms an angle of 70-110 ° with the pressing hand body, and the angle formed by the pressing hand head and the pressing hand body faces in the opposite direction to the angle formed by the pressing hand tail and the pressing hand body.
In another preferred embodiment, the angle formed by the hand pressing head and the hand pressing body faces the micro-fluidic chip positioning tray.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with a pressing hole for pressing the centrifugal pressing hand shaft.
In another preferred embodiment, the pressing hole is a pressing threaded hole.
In another preferred embodiment, the centrifugal pressing hand shaft is screwed on the pressing threaded hole through a screw for pressing the chip.
In another preferred embodiment of the present invention, the centrifugal microfluidic chip positioning tray is provided with positioning pins. The locating pin is used for locating the centrifugal flow control chip, so that confusion is prevented. The number of the positioning pins is preferably 2-6.
In another preferred embodiment of the present invention, the centrifugal microfluidic chip positioning tray is provided with a plurality of elongated holes. The detection equipment can detect the centrifugal microfluidic chip through the strip-shaped holes through the centrifugal microfluidic chip positioning tray.
In another preferred embodiment of the present invention, the transmission part includes a motor and a rotation shaft. Typically, the rotating shaft is connected to the motor, and the centrifugal microfluidic chip positioning tray is connected to the rotating shaft.
In another preferred embodiment, the chip is a cylindrical shaft of the centrifugal press hand shaft.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is disc-shaped.
In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with a mounting groove, and the centrifugal hand pressing shaft is arranged on the mounting groove.
Centrifugal micro-fluidic chip
In order to overcome the defects that gel needs to be filled into an electrophoresis tank manually every time and only one electrophoresis tank can be filled at a time in the conventional gel electrophoresis experiment, the invention develops a centrifugal microfluidic chip capable of automatically filling the gel into a plurality of gel channels at one time through centrifugation.
The centrifugal microfluidic chip of the present invention will be described below with reference to fig. 7-11, it being understood that the drawings are for illustrative purposes only and are not to be construed as limiting the patent.
Typically, a centrifugal microfluidic chip comprising a channel plate 2.1;
The channel disc is provided with a plurality of liquid storage cavities 2.2, the central area of the channel disc is provided with a circular reagent cavity, each liquid storage cavity is communicated with the circular reagent cavity 2.3, each liquid storage cavity is connected with a main micro-channel 2.4, the tail end of the main micro-channel far away from the liquid storage cavity is communicated with a first exhaust cavity through a main pore canal 2.5, one end of the main micro-channel close to the main pore canal is provided with a left micro-channel 2.6 and a right micro-channel 2.7, and the tail ends of the left micro-channel and the right micro-channel are respectively communicated with a second exhaust cavity and a third exhaust cavity through a left pore canal 2.8 and a right pore canal 2.9;
The channel plate is provided with a liquid adding port 2.10 which is communicated with the liquid storage cavity or the circular reagent cavity, and the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with an exhaust hole 2.11.
In a preferred embodiment, the channel plate is a circular plate.
In another preferred embodiment, the number of the liquid adding ports is 1 or more, preferably 2-6, and more preferably 2-3.
When there are a plurality of filling openings, gel can be added into the liquid storage cavity and/or the circular reagent cavity from one of the filling openings, and when the gel flows out from the other filling opening, the gel in the liquid storage cavity and/or the circular reagent cavity is full.
In another preferred embodiment, the liquid filling opening is provided with a glue film 18. The glue film can avoid the pollution of the outside to the cavity of the channel disc and the channel.
According to the centrifugal microfluidic chip, the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with the exhaust holes, so that air in the main micro-channel, the left micro-channel and the right micro-channel in the gel filling process can be smoothly exhausted, gel impurity cleaning bubbles filled in the main micro-channel, the left micro-channel and the right micro-channel can be avoided, and the gel filling quality is ensured.
In the present invention, the positions of the liquid storage chamber, the circular reagent chamber, the main microchannel, the left microchannel, the right microchannel, the main channel, the left channel, the right channel, the first exhaust chamber, the second exhaust chamber, and/or the third exhaust chamber in the channel tray are not particularly limited as long as the objects of the present invention are satisfied.
In a preferred embodiment of the present invention, the liquid storage chamber, the circular reagent chamber, the main microchannel, the left microchannel and/or the right microchannel are provided near the lower surface side of the channel plate.
In another preferred embodiment, the first exhaust chamber, the second exhaust chamber and/or the third exhaust chamber are provided near the upper surface side of the passage plate.
In the present invention, the number of the liquid storage chambers is not particularly limited, and may be designed according to the purpose of experiments, and it is preferable that the number of the liquid storage chambers is 2 to 60, preferably 6 to 40, more preferably 8 to 30, and most preferably 10 to 30.
In a preferred embodiment of the invention, the primary microchannel is less hydrophilic than the reservoir. Typically, each primary microchannel is less hydrophilic than the reservoir to which it is connected. The hydrophilicity of the main micro-channel is smaller than that of the liquid storage cavity, so that the gel can simultaneously fill the main micro-channel, the left micro-channel and the right micro-channel when the liquid storage cavity is centrifuged, and the gel simultaneously enters the first air discharge cavity, the second air discharge cavity and the third air discharge cavity from the main pore channel, the left pore channel and the right pore channel. Experimental research shows that if the hydrophilicity of part of the main micro-channels is equal to or greater than that of the liquid storage cavity connected with the main micro-channels, gel can not be ensured to be filled in all the main micro-channels, the left micro-channel and the right micro-channel at the same time during centrifugation, the part of the main micro-channels can enter the first air exhaust cavity, the second air exhaust cavity and the third air exhaust cavity in advance before or during centrifugation, even the polluted instrument is discharged from the air exhaust holes in advance, so that gel filling fails,
In another preferred embodiment, the liquid storage cavity is made of hydrophilic material or the surface is subjected to hydrophilic treatment. In another preferred embodiment, the main micro-channel is a hydrophobic material or the surface is subjected to a hydrophobic treatment.
In a preferred embodiment of the invention, the individual reservoirs are arranged evenly around the circular reagent chamber. In another preferred embodiment, the center (center) of the channel plate coincides with the center (center) of the circular reagent chamber. In the present invention, it should be understood that the coincidence of the centers (circle centers) also means that the channel plate and the circular reagent chamber form concentric circles.
In another preferred embodiment, the main, left and/or right micro-channels are straight channels.
In another preferred embodiment, the longitudinal cross-section of the main microchannel, the left microchannel and/or the right microchannel is circular, rectangular or square.
In a preferred embodiment of the present invention, the main micro-channels are distributed (extended) along the center of the channel plate in a direction toward the outer periphery of the channel plate.
In another preferred embodiment, the area S1 of the longitudinal section of the main microchannel is 0.002 mm 2≤S1≤0.005mm2.
In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.003 mm 2≤S2≤0.007mm2.
In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.003 mm 2≤S3≤0.007mm2.
In another preferred embodiment, the longitudinal section of the left micro-channel is rectangular, the length is 0.10-0.14mm, and the width is 0.03-0.05mm.
In another preferred embodiment, the longitudinal section of the main micro-channel is rectangular, and the length is 0.06-0.10mm, and the width is 0.03-0.05mm.
In another preferred embodiment, the right micro-channel has a rectangular longitudinal section, a length of 0.10-0.14mm and a width of 0.03-0.05mm.
In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.8-2 times, preferably 1.0-2 times, more preferably 1.2-1.8 times, still more preferably 1.3-1.5 times, most preferably 1.4-1.6 times the area S1 of the section of the main microchannel.
In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.8-2 times, preferably 1.0-2 times, more preferably 1.2-1.8 times, still more preferably 1.3-1.5 times, most preferably 1.4-1.6 times the area S1 of the longitudinal section of the main microchannel.
Preferably, the included angle α1 between the left microchannel and the main microchannel is equal to the included angle α2 between the right microchannel and the main microchannel.
The inventor finds through a great deal of experimental study that when the included angle alpha 1 between the left micro-channel and the main micro-channel and the included angle alpha 2 between the right micro-channel and the main micro-channel are not in the range of 56+/-0.5 degrees, gel discharge occurs in the exhaust holes of a part of a plurality of first exhaust cavities, second exhaust cavities and third exhaust cavities (the discharge holes of the gel can pollute a centrifugal microfluidic chip and a centrifugal device and stop centrifugation) when the gel is centrifugally filled, and gel does not appear in the rest of the first exhaust cavities, the second exhaust cavities and the third exhaust cavities, so that the main micro-channel, the left micro-channel and the right micro-channel are unfilled, and the requirement of filling all the main micro-channel, the left micro-channel and the right micro-channel cannot be met, and gel filling fails.
In another preferred example, each liquid storage cavity is a long hole type liquid storage cavity, and the dimensional tolerance of the long hole type liquid storage cavity is 0.015-0.025mm, and the depth is 4.8-5.2mm. A typical slot-type reservoir is shown in figure 11.
The gel in the long hole type liquid storage cavity is concentrated, and the gel can enter the main micro-channel more effectively when the gel is centrifuged because the stress point, namely the main micro-channel opening, can receive larger centrifugal force.
In another preferred embodiment, the circular reagent chambers are raised in the channel plate.
In another preferred embodiment, the channel plate is further provided with positioning holes 2.19. The positioning holes can be combined with positioning pins on the centrifugal microfluidic chip clamping device to prevent the position relation of each channel of the centrifugal microfluidic chip from being confused.
Centrifugal microfluidic chip preparation method
The preparation method of the centrifugal microfluidic chip is not particularly limited, and can be prepared by adopting a method commonly used in the prior art, such as mechanical cutting, film lamination and the like, for example, preparing an upper film, a middle sheet (the middle sheet is provided with a plurality of cavity grooves, channel grooves and the like) and a lower film, and punching prepared liquid inlets and air outlets after the upper film and the lower film are laminated with the middle sheet to prepare the centrifugal microfluidic chip.
Gel electrophoresis apparatus
The invention also provides gel electrophoresis equipment, which comprises the centrifugal microfluidic chip clamping device and the centrifugal microfluidic chip.
In another preferred embodiment, the centrifugal microfluidic chip is mounted on the centrifugal microfluidic chip positioning tray.
In the gel electrophoresis device, the centrifugal microfluidic chip clamping device and the centrifugal microfluidic chip are as described above.
The main advantages of the invention include:
1. The centrifugal microfluidic chip clamping device clamps the centrifugal microfluidic chip in a centrifugal clamping mode, and the larger the centrifugal force of the centrifugal microfluidic chip clamping device is, the larger the clamping force of the centrifugal pressing hand on the centrifugal microfluidic chip is, so that the centrifugal microfluidic chip can be prevented from falling off in the centrifugal process.
2. In the centrifugal microfluidic chip clamping device, the centrifugal pressing hand is in a hanging state in an unfiugued state, so that the centrifugal microfluidic chip can be conveniently placed and taken out, and the phenomenon that the centrifugal microfluidic chip is difficult to place and take out in the microfluidic chip positioning tray is avoided.
3. In the centrifugal microfluidic core clamping device, the centrifugal microfluidic chip can be directly placed on the microfluidic chip positioning tray for centrifugation, and a rotating shaft mounting hole is not required to be additionally arranged at the center of the centrifugal microfluidic chip, so that compared with the conventional microfluidic chip hole with the rotating shaft mounting hole arranged at the center, the centrifugal microfluidic core clamping device is applicable to the centrifugal microfluidic chip, and the channel length of the centrifugal microfluidic chip can be prevented from being too small while the size is reduced.
4. In the centrifugal microfluidic chip, a proper amount of gel is filled into the centrifugal microfluidic chip through the charging hole of the channel disc at one time only by manually using a liquid-transferring gun, then the centrifugal microfluidic chip is placed in the existing centrifugal device, the gel is filled into the corresponding main micro-channel, the left micro-channel and the right micro-channel through centrifugation, and when the gel is observed to appear in the first exhaust cavity, the second exhaust cavity and the third exhaust cavity, the gel filling is finished, and finally, a gel electrophoresis experiment is completed through a series of automatic operations, so that the centrifugal microfluidic chip provided by the invention can rapidly and accurately realize the filling of the multichannel gel, and the experimental (electrophoresis) efficiency is improved.
5. When the centrifugal microfluidic chip fills gel through centrifugation, air in the main microchannel, the left microchannel and the right microchannel is discharged through the exhaust holes of the exhaust cavity under the action of centrifugation, so that gel impurity-removing bubbles filled in the main microchannel, the left microchannel and the right microchannel can be avoided, and the gel filling quality is ensured.
6. Compared with the traditional microfluidic chip hole with the rotary shaft mounting hole at the center, the centrifugal microfluidic chip has a longer channel and a wider application range, so that the diameter of the microfluidic chip can be greatly reduced, and the centrifugal microfluidic chip has a separation channel with enough length during DNA and protein gel electrophoresis.
The invention will be further illustrated with reference to specific examples. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. The experimental methods, in which specific conditions are not noted in the following examples, are generally conducted under conventional conditions or under conditions recommended by the manufacturer. Percentages and parts are by weight unless otherwise indicated.
Example 1
The embodiment 1 provides a centrifugal microfluidic chip clamping device, which comprises a centrifugal microfluidic chip positioning part and a transmission part;
The centrifugal microfluidic chip positioning part comprises a centrifugal microfluidic chip positioning tray 1.1, a centrifugal hand pressing shaft 1.2 and a centrifugal hand pressing 1.3, wherein the centrifugal hand pressing is provided with a through hole 1.4, the centrifugal hand pressing is a Z-shaped centrifugal hand pressing, the Z-shaped centrifugal hand pressing comprises a hand pressing head 1.6, a hand pressing body 1.7 and a hand pressing tail 1.8, the hand pressing head and the hand pressing body form an angle of 105 degrees, the hand pressing tail and the hand pressing body form an angle of 95 degrees, the angle formed by the hand pressing head and the hand pressing body and the angle formed by the hand pressing tail and the hand pressing body face in opposite directions, and the angle formed by the hand pressing head and the hand pressing body faces the microfluidic chip positioning tray;
the periphery of the centrifugal microfluidic chip positioning tray is provided with 3 hanging through holes 1.5 and 2 positioning pins, the centrifugal hand pressing shaft penetrates through the through holes of the centrifugal hand pressing shaft, two ends of the centrifugal hand pressing shaft penetrate through the hanging through holes, and the centrifugal hand pressing shaft can rotate around the centrifugal hand pressing shaft;
In the non-centrifugal state, the centrifugal press hand is in a hanging state, and the gravity center of the centrifugal press hand is positioned below the perforation;
in the centrifugal state, the centrifugal press hand can rotate outwards and upwards around the centrifugal press hand shaft, and the upper end of the centrifugal press hand can rotate inwards and downwards;
The transmission part comprises a motor 1.10 and a rotating shaft 1.11, the rotating shaft is connected with the motor, the microfluidic chip positioning tray is connected with the rotating shaft, and the transmission part can drive the centrifugal microfluidic chip positioning tray to rotate;
The centrifugal microfluidic chip positioning tray also is provided with a pressing hole 1.9, a strip-shaped hole and a mounting groove, wherein the pressing hole is used for pressing the centrifugal pressing hand shaft, the centrifugal pressing hand shaft is arranged on the mounting groove, and the detection equipment can detect the centrifugal microfluidic chip through the strip-shaped hole through the centrifugal microfluidic chip positioning tray.
Fig. 1 and 2 are schematic structural diagrams of a centrifugal microfluidic chip positioning tray and a centrifugal pressing hand, fig. 3 and 4 are schematic structural diagrams of a centrifugal microfluidic chip clamping device provided with a centrifugal microfluidic chip, and fig. 5 and 6 are the centrifugal pressing hand states in the non-centrifugal and centrifugal states, respectively.
The working principle of the microfluidic chip clamping device in embodiment 1 mainly comprises:
In the non-centrifugal state, the centrifugal pressing hand is in a hanging state (as shown in fig. 5), so that the centrifugal microfluidic chip is conveniently placed on the centrifugal microfluidic chip positioning tray, and the positioning pin can be combined with the positioning hole of the centrifugal microfluidic chip positioning tray for positioning the centrifugal microfluidic chip to prevent confusion; when the motor drives the centrifugal microfluidic chip positioning tray to rotate at a high speed through the rotating shaft, under the action of centrifugal force, the centrifugal press hand can rotate outwards and upwards around the lower end of the centrifugal press hand shaft, and the upper end of the centrifugal press hand rotates inwards and downwards (as shown in fig. 6), so that the centrifugal press hand clamps the centrifugal microfluidic chip, pressure is generated on the centrifugal microfluidic chip, the centrifugal microfluidic chip is prevented from falling off from the microfluidic chip positioning tray in the centrifugal process, the larger the rotating speed of the centrifugal microfluidic chip positioning tray is, the larger the centrifugal force is, the stronger the rotation of the centrifugal press hand is, and the pressure generated on the centrifugal microfluidic chip is prevented from falling off due to high-speed centrifugation.
Example 2
Embodiment 2 provides a centrifugal microfluidic chip, the schematic structural diagram of which is shown in fig. 7-10, wherein the centrifugal microfluidic chip comprises a circular channel disc 2.1;
The channel plate is provided with a plurality of liquid storage cavities 2.2, the central area of the channel plate is provided with a circular reagent cavity, each liquid storage cavity is communicated with the circular reagent cavity 2.3, each liquid storage cavity is uniformly arranged around the circular reagent cavity, the circular reagent cavity is protruded on the channel plate, the center of the channel plate coincides with the center of the circular reagent cavity, each liquid storage cavity is connected with a main micro-channel 2.4, the main micro-channels are distributed along the center of the channel plate towards the peripheral direction of the channel plate, the tail end of the main micro-channel far away from the liquid storage cavity is communicated with a first exhaust cavity through a main channel 2.5, one end of the main micro-channel close to the main channel is provided with a left micro-channel 2.6 and a right micro-channel 2.7, and the tail ends of the left micro-channel and the right micro-channel are respectively communicated with a second exhaust cavity and a third exhaust cavity through a left micro-channel 2.8 and a right micro-channel 2.9;
the channel disc is provided with 2 liquid adding ports 2.10 which are communicated with the liquid storage cavity, and the liquid adding ports are provided with adhesive films 2.18;
the first exhaust cavity comprises a first exhaust channel 2.12 and a first excess reagent cavity 2.13, the second exhaust cavity comprises a second exhaust channel 2.14 and a second excess reagent cavity 15, the third exhaust cavity comprises a third exhaust channel 2.16 and a third excess reagent cavity 2.17, and the first excess reagent cavity, the second excess reagent cavity and the third excess reagent cavity are respectively provided with exhaust holes 2.11; the main pore canal is communicated with the first excessive reagent cavity through a first exhaust channel, the left pore canal is communicated with the second excessive reagent cavity through a second exhaust channel, and the right pore canal is communicated with the third excessive reagent cavity through a third exhaust channel;
the hydrophilicity of each main micro-channel is smaller than that of a liquid storage cavity connected with the main micro-channel, and the hydrophilicities of the main micro-channel, the left micro-channel and the right micro-channel are the same;
The main micro-channel, the left micro-channel and/or the right micro-channel are/is straight channels, the longitudinal section of the left micro-channel is rectangular, the length is 0.12mm, the width is 0.04mm, the longitudinal section of the main micro-channel is rectangular, the length is 0.08mm, the width is 0.4mm, the longitudinal section of the right micro-channel is rectangular, the length is 0.12mm, and the width is 0.04mm;
The included angle alpha 1 between the left micro-channel and the main micro-channel is 56 degrees, and the included angle alpha 2 between the right micro-channel and the main micro-channel is 56 degrees;
the channel plate is also provided with a positioning hole 2.19.
Example 2 centrifugal microfluidic chip gel filling procedure
After the gel is filled in each liquid storage cavity and the circular reagent cavity, placing the centrifugal microfluidic chip in a centrifugal device, centrifuging, filling the gel in the liquid storage cavity and the circular reagent cavity into the corresponding main micro-channel, left micro-channel and right micro-channel, after the gel is filled in the main micro-channel, left micro-channel and right micro-channel, almost simultaneously entering the first exhaust cavity, the second exhaust cavity and the third exhaust cavity through the main pore channel, the left pore channel and the right pore channel, namely, when the gel is observed in the first exhaust cavity, the second exhaust cavity and the third exhaust cavity, the main micro-channel, the left micro-channel and the right micro-channel are filled with the gel, stopping centrifuging, and obtaining the microfluidic chip with the main micro-channel, the left micro-channel and the right micro-channel filled with the gel, thereby realizing one-time feeding, and fast and accurate filling of the gel, and erasing bubbles of the gel filled in the main micro-channel, the left micro-channel and the right micro-channel, and ensuring the quality of gel filling.
Example 2 centrifugal microfluidic chip DNA electrophoresis experiments
After the main, left and right micro-channels of the centrifugal microfluidic chip of example 2 were filled with gel, the instrument electrode pierced the left, main and right channels simultaneously; then the instrument automatically adds a sample to be measured containing DNA into the left channel, then the instrument electrode is inserted into the left channel and the right channel, the left channel is negatively charged, the right channel is positively charged, and the DNA sample is negatively charged, so that the sample can flow through the intersection of the main micro channel, the left micro channel and the right micro channel under the action of Lorentz force; disconnecting the electrode; and then electrodes are inserted into the main channel and the liquid filling opening, the liquid filling opening is connected with a positive electrode, the main channel is connected with negative electricity, under the action of Lorentz force, a sample at the intersection of the left channel, the main channel and the right channel flows into the main micro channel, the sample is combined with a pre-coated reagent in the main micro channel, a specific wavelength can be generated under the irradiation of laser with the specific wavelength, the wavelength is received by an optical sensor, a corresponding map is produced under the treatment of software, and a tester can effectively judge the sample according to the map.
Comparative example 1
Comparative example 1 provides a centrifugal microfluidic chip, the centrifugal microfluidic chip of comparative example 1 is similar to the centrifugal microfluidic chip of example 2 in structure, and the centrifugal microfluidic chip of comparative example 1 is compared with the centrifugal microfluidic chip of example 2 in distinguishing technical characteristics: in the centrifugal microfluidic chip of comparative example 1, the hydrophilicity of a portion of the main micro-channels is equal to or greater than the hydrophilicity of the reservoir chambers connected thereto.
In the gel filling process of the centrifugal microfluidic chip of comparative example 1, it cannot be ensured that the gel can simultaneously fill all the main micro-channels, the left micro-channel and the right micro-channel during centrifugation, and the main micro-channels (with hydrophilicity equal to or greater than that of the liquid storage cavity connected with the main micro-channels) can enter the first air exhaust cavity, the second air exhaust cavity and the third air exhaust cavity in advance before or during centrifugation, and even discharge the polluted instrument from the air exhaust holes in advance, so that gel filling fails.
Comparative example 2 and comparative example 3
Comparative example 2 provides a centrifugal microfluidic chip, the centrifugal microfluidic chip of comparative example 2 is similar to the centrifugal microfluidic chip of example 2 in structure, and the centrifugal microfluidic chip of comparative example 2 is characterized in that: in the centrifugal microfluidic chip of comparative example 2, the included angle α1 between the left and main microchannels was 54 °, and the included angle α2 between the right and main microchannels was 54 °.
Comparative example 3 provides a centrifugal microfluidic chip, the centrifugal microfluidic chip of comparative example 3 is similar to the centrifugal microfluidic chip of example 2 in structure, and the centrifugal microfluidic chip of comparative example 3 is compared with the centrifugal microfluidic chip of example 2 in distinguishing technical characteristics: in the centrifugal microfluidic chip of comparative example 3, the included angle α1 between the left microchannel and the main microchannel was 58 °, and the included angle α2 between the right microchannel and the main microchannel was 58 °.
In the gel filling process of the centrifugal microfluidic chip of comparative example 2 and comparative example 3, gel cannot enter the first exhaust cavity, the second exhaust cavity and the third exhaust cavity at the same time, when gel discharge occurs in part of the exhaust holes of the first exhaust cavity, the second exhaust cavity and the third exhaust cavity (gel discharge from the exhaust holes can pollute the centrifugal microfluidic chip and a centrifugal device and stop centrifugation), and gel does not occur in the rest of the first exhaust cavity, the second exhaust cavity and the third exhaust cavity, so that the main micro-channel, the left micro-channel and the right micro-channel are not filled, and the requirement of filling all the main micro-channel, the left micro-channel and the right micro-channel cannot be met, and gel filling fails.
Example 3
Embodiment 3 provides a gel electrophoresis apparatus, which is composed of the centrifugal microfluidic chip clamping device prepared in embodiment 1 and the centrifugal microfluidic chip prepared in embodiment 2;
The positioning pins on the centrifugal microfluidic chip positioning tray are combined with the positioning holes of the centrifugal microfluidic chip, so that the centrifugal microfluidic chip is arranged on the centrifugal microfluidic chip positioning tray (shown in fig. 3 or fig. 4), after gel is added through the liquid adding port of the channel tray, when the motor drives the centrifugal microfluidic chip positioning tray to rotate at a high speed through the rotating shaft, the main microchannel, the left microchannel and the right microchannel on the centrifugal microfluidic chip are rapidly filled with the gel at one time under the action of centrifugal force, and a DNA electrophoresis experiment is carried out.
All documents mentioned in this disclosure are incorporated by reference in this disclosure as if each were individually incorporated by reference. Further, it will be appreciated that various changes and modifications may be made by those skilled in the art after reading the above teachings, and such equivalents are intended to fall within the scope of the application as defined in the appended claims.
Claims (8)
1. The centrifugal microfluidic chip clamping device is characterized by comprising a centrifugal microfluidic chip positioning part and a transmission part;
The centrifugal microfluidic chip positioning part comprises a centrifugal microfluidic chip positioning tray (1.1), a centrifugal pressing hand shaft (1.2) and a centrifugal pressing hand (1.3), wherein the centrifugal pressing hand is provided with a perforation (1.4);
The periphery of the centrifugal microfluidic chip positioning tray is provided with a hanging through hole (1.5), the centrifugal hand pressing shaft penetrates through the through hole of the centrifugal hand pressing shaft, two ends of the centrifugal hand pressing shaft penetrate through the hanging through hole, and the centrifugal hand pressing shaft can rotate around the centrifugal hand pressing shaft;
in the non-centrifugal state, the centrifugal pressing hand is in a hanging state, and the gravity center of the centrifugal pressing hand is positioned below the perforation; in the centrifugal state, the centrifugal press hand can rotate outwards and upwards around the centrifugal press hand shaft, and the upper end of the centrifugal press hand can rotate inwards and downwards;
The centrifugal microfluidic chip positioning tray is connected with the transmission part, and the transmission part can drive the centrifugal microfluidic chip positioning tray to rotate;
The centrifugal press hand is a Z-shaped centrifugal press hand, the Z-shaped centrifugal press hand comprises a press hand head (1.6), a press hand body (1.7) and a press hand tail (1.8), the press hand head and the press hand body form an angle of 50-110 degrees, the press hand tail and the press hand body form an angle of 70-110 degrees, and the angle formed by the press hand head and the press hand body and the angle formed by the press hand tail and the press hand body face opposite directions.
2. The centrifugal microfluidic chip clamping device according to claim 1, wherein the number of the hanging through holes is 2-8.
3. The centrifugal microfluidic chip clamping device according to claim 1, wherein the number of the hanging through holes is 3-6.
4. The centrifugal microfluidic chip clamping device according to claim 1, wherein the angle formed by the pressing head and the pressing body faces the microfluidic chip positioning tray.
5. The centrifugal microfluidic chip clamping device according to claim 1, wherein the centrifugal microfluidic chip positioning tray is further provided with a pressing hole (1.9) for pressing the centrifugal pressing hand shaft.
6. The centrifugal microfluidic chip clamping device according to claim 1, wherein the transmission part comprises a motor (1.10) and a rotating shaft (1.11).
7. The centrifugal microfluidic chip clamping device according to claim 1, wherein the centrifugal microfluidic chip positioning tray is provided with positioning pins (1.12).
8. The centrifugal microfluidic chip clamping device according to claim 1, wherein the centrifugal microfluidic chip positioning tray is provided with a plurality of elongated holes.
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