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CN208632559U - A kind of biological cell breeding apparatus - Google Patents

A kind of biological cell breeding apparatus Download PDF

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Publication number
CN208632559U
CN208632559U CN201820423050.4U CN201820423050U CN208632559U CN 208632559 U CN208632559 U CN 208632559U CN 201820423050 U CN201820423050 U CN 201820423050U CN 208632559 U CN208632559 U CN 208632559U
Authority
CN
China
Prior art keywords
culture chamber
oxygen
culture
mounting seat
breeding apparatus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201820423050.4U
Other languages
Chinese (zh)
Inventor
李娜
王强
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanxi Agricultural University
Original Assignee
Shanxi Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanxi Agricultural University filed Critical Shanxi Agricultural University
Priority to CN201820423050.4U priority Critical patent/CN208632559U/en
Application granted granted Critical
Publication of CN208632559U publication Critical patent/CN208632559U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a kind of biological cell breeding apparatus of field of biotechnology, including mounting seat, the top left and right ends of the mounting seat are respectively connected with bracket, axis pin base is installed at the top center of the mounting seat, the top of the axis pin base is connected with axle sleeve by pin shaft, sucker is connected at the top of the axle sleeve, culture chamber is connected at the top of the sucker, the left and right sidewall of the culture chamber is respectively connected with reset spring, the other end of reset spring described in two groups is connect with the side wall of two pack supports respectively, the biological cell breeding apparatus, it is carried out by way of shake to culture chamber vibration, cell will be cultivated to separate, increase the activity of culture cell, oxygen conveying pipe is plugged into the intracavity bottom of culture chamber, increase the contact area of oxygen and culture solution.

Description

A kind of biological cell breeding apparatus
Technical field
The utility model relates to field of biotechnology, specially a kind of biological cell breeding apparatus.
Background technique
Biotechnology and biologic medical science are paid attention in current development in science and technology is Sino-Japan, and in numerous scientific research activities, training Zooblast is planted, the cell particularly for human body is even more one of project of greatest concern, either studies cell behavior, using thin Born of the same parents itself or the byproduct for using cellular activity, the cell supply for requiring to have both quality, quantity and efficiency are supported that cell is trained It plants and needs to carry out cell separation by way of stirring, be easy to cause the rupture of cell, for this purpose, we have proposed a kind of biology is thin Born of the same parents' implement for cultivating.
Utility model content
The purpose of this utility model is to provide a kind of biological cell breeding apparatus, to solve to propose in above-mentioned background technique Existing implement for cultivating for carried out by way of stirring increase culture solution oxygen content, be easy to cause the rupture of cell Problem.
To achieve the above object, the utility model provides the following technical solutions: a kind of biological cell breeding apparatus, including peace Pedestal is filled, the top left and right ends of the mounting seat are respectively connected with bracket, are equipped at the top center of the mounting seat The top of axis pin base, the axis pin base is connected with axle sleeve by pin shaft, and sucker is connected at the top of the axle sleeve, the sucker Top is connected with culture chamber, and the left and right sidewall of the culture chamber is respectively connected with reset spring, reset spring described in two groups The other end is connect with the side wall of two pack supports respectively, and the inner cavity left side wall of the culture chamber is connected with nutrient solution inlet pipe, described Left side wall of the top left side of nutrient solution inlet pipe through culture chamber, the flexible rubber of top left side grafting of the nutrient solution inlet pipe Glue sealing-plug is provided with top cover at the top of the culture chamber, and the top right side of the top cover is plugged with oxygen inlet, the oxygen Gas import runs through top cover, and the bottom of the oxygen inlet is connected with oxygen conveying pipe, and the top left side of the top cover is plugged with row Port, and the bottom of exhaust outlet is connected to the inner cavity of culture chamber, the left side of the exhaust outlet is connected with suction pump by pipeline, The air inlet of the suction pump and the top of exhaust outlet connect, and are horizontally installed with culture plate on the downside of the inner cavity of the culture chamber.
Preferably, the bottom four corners of the mounting seat pass through bonding agent and are bonded with rubber antiskid pad.
Preferably, dust-proof sieve is connected at the top of the gas outlet of the suction pump.
Preferably, the bottom of the nutrient solution inlet pipe and oxygen conveying pipe is plugged into the intracavity bottom of culture chamber.
Compared with prior art, the utility model has the beneficial effects that the biological cell breeding apparatus, passes through the shape of shake Formula culture chamber vibration will be cultivated cell and be separated, increases the activity of culture cell, oxygen conveying pipe is plugged into The intracavity bottom of chamber is cultivated, the contact area of oxygen and culture solution is increased.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model.
In figure: 1 mounting seat, 2 brackets, 3 axis pin bases, 4 axle sleeves, 5 suckers, 6 culture chambers, 7 reset springs, 8 nutrient solutions Inlet pipe, 9 elastic rubber sealing-plugs, 10 oxygen inlets, 11 oxygen conveying pipes, 12 exhaust outlets, 13 suction pumps, 14 top covers, 15 cultures Plate.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.
Referring to Fig. 1, the utility model provides a kind of technical solution: a kind of biological cell breeding apparatus, including installation bottom Seat 1, the top left and right ends of mounting seat 1 are respectively connected with bracket 2, and axis pin base 3 is equipped at the top center of mounting seat 1, The top of axis pin base 3 is connected with axle sleeve 4 by pin shaft, and the top of axle sleeve 4 is connected with sucker 5, and the top of sucker 5 is connected with culture The left and right sidewall of chamber 6, culture chamber 6 is respectively connected with reset spring 7, the other end of two groups of reset springs 7 respectively with two groups of branch The side wall of frame 2 connects, and the inner cavity left side wall of culture chamber 6 is connected with nutrient solution inlet pipe 8, and the top left side of nutrient solution inlet pipe 8 is passed through The left side wall of culture chamber 6 is worn, the top left side of nutrient solution inlet pipe 8 is plugged with elastic rubber sealing-plug 9, cultivates the top of chamber 6 Portion is provided with top cover 14, and the top right side of top cover 14 is plugged with oxygen inlet 10, and oxygen inlet 10 runs through top cover 14, oxygen inlet 10 bottom is connected with oxygen conveying pipe 11, and the top left side of top cover 14 is plugged with exhaust outlet 12, and the bottom of exhaust outlet 12 with The inner cavity connection of chamber 6 is cultivated, the left side of exhaust outlet 12 is connected with suction pump 13, the air inlet of suction pump 13 and row by pipeline The top of port 12 connects, and cultivates and is horizontally installed with culture plate 15 on the downside of the inner cavity of chamber 6.
Wherein, the bottom four corners of mounting seat 1 pass through bonding agent and are bonded with rubber antiskid pad, avoid mounting seat 1 sliding It is dynamic, it is connected with dust-proof sieve at the top of the gas outlet of suction pump 13, increases the cleaning of culture 6 inner cavity of chamber, nutrient solution inlet pipe 8 and oxygen The bottom of letter shoot 11 is plugged into the intracavity bottom of culture chamber 6.
Working principle: culture chamber 6 uses transparent glassware, convenient for observing internal configuring condition, in culture chamber 6 Inner cavity have a culture solution, culture plate 15 is located at culture solution hereinafter, cell to be cultivated is placed on culture plate 15, to the left or to Chamber 6 is cultivated in right drawing, and culture chamber 6 is rotated clockwise or counter-clockwise by 5 suckers 5 around axle sleeve 4, and culture chamber 6 rotates To after certain angle, culture chamber 6 is unclamped, under the action of two groups of reset spring 7, culture chamber 6 starts reciprocal left-right rotation Effect, so that the cell on 15 surface of culture plate be separated, can normally cultivate development, the surface of culture plate 15 uniformly opens up The external oxygen conveying device of oxygen inlet 10 is carried out oxygen input, oxygen is transported to training by oxygen conveying pipe 11 by through-hole Hereinafter, the bottom end of oxygen conveying pipe 11 is located at the downside of culture plate 15, oxygen increases under the baffle effect of culture plate 15 in nutrient solution face Add the time of contact with nutrient solution, to improve the joint efficiency of oxygen and nutrient solution, the oxygen in nutrient solution passes through culture plate Through-hole on 15 moves up so that cultivate cell can normal aerobic respiration, over time, need by Nutrient solution is gradually added, the injection of the enterprising field headquarters nutrient solution of elastic rubber sealing-plug 9 is plugged by syringe, nutrient solution inlet pipe 8 will Nutrient solution is output to culture liquid level hereinafter, the bottom end of nutrient solution inlet pipe 8 is located at the upside of culture plate 15, convenient for cultivating the straight of cell Intake is connect, after injection, syringe is extracted, elastic rubber sealing-plug 9 can be closed the slotting hole of syringe, respiration automatically The CO of generation2Periodically discharge, when exhaust, suction pump 13 will cultivate the CO of 6 inner cavity of chamber by exhaust outlet 122And excessive gas Extraction.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (4)

1. a kind of biological cell breeding apparatus, including mounting seat (1), it is characterised in that: the top left of the mounting seat (1) Right both ends are respectively connected with bracket (2), are equipped with axis pin base (3), the axis pin base at the top center of the mounting seat (1) (3) top is connected with axle sleeve (4) by pin shaft, is connected with sucker (5) at the top of the axle sleeve (4), the top of the sucker (5) Portion is connected with culture chamber (6), and the left and right sidewall of culture chamber (6) is respectively connected with reset spring (7), resets described in two groups The other end of spring (7) is connect with the side wall of two pack supports (2) respectively, and the inner cavity left side wall of culture chamber (6) is connected with Nutrient solution inlet pipe (8), the top left side of the nutrient solution inlet pipe (8) through culture chamber (6) left side wall, the nutrient solution into The top left side of pipe (8) is plugged with elastic rubber sealing-plug (9), is provided with top cover (14), institute at the top of the culture chamber (6) The top right side for stating top cover (14) is plugged with oxygen inlet (10), and the oxygen inlet (10) runs through top cover (14), the oxygen The bottom of import (10) is connected with oxygen conveying pipe (11), and the top left side of the top cover (14) is plugged with exhaust outlet (12), and The bottom of exhaust outlet (12) is connected to the inner cavity of culture chamber (6), and the left side of the exhaust outlet (12) is connected with pumping by pipeline The air inlet of sucking pump (13), the suction pump (13) is connect with the top of exhaust outlet (12), under the inner cavity of culture chamber (6) Side is horizontally installed with culture plate (15).
2. a kind of biological cell breeding apparatus according to claim 1, it is characterised in that: the bottom of the mounting seat (1) Portion quadrangle passes through bonding agent and is bonded with rubber antiskid pad.
3. a kind of biological cell breeding apparatus according to claim 1, it is characterised in that: the outlet of the suction pump (13) The top of mouth is connected with dust-proof sieve.
4. a kind of biological cell breeding apparatus according to claim 1, it is characterised in that: the nutrient solution inlet pipe (8) with The bottom of oxygen conveying pipe (11) is plugged into the intracavity bottom of culture chamber (6).
CN201820423050.4U 2018-03-26 2018-03-26 A kind of biological cell breeding apparatus Expired - Fee Related CN208632559U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820423050.4U CN208632559U (en) 2018-03-26 2018-03-26 A kind of biological cell breeding apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820423050.4U CN208632559U (en) 2018-03-26 2018-03-26 A kind of biological cell breeding apparatus

Publications (1)

Publication Number Publication Date
CN208632559U true CN208632559U (en) 2019-03-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201820423050.4U Expired - Fee Related CN208632559U (en) 2018-03-26 2018-03-26 A kind of biological cell breeding apparatus

Country Status (1)

Country Link
CN (1) CN208632559U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113447645A (en) * 2021-06-11 2021-09-28 青岛大学附属医院 Auxiliary taking and placing device for culture bottle samples for medical examination

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113447645A (en) * 2021-06-11 2021-09-28 青岛大学附属医院 Auxiliary taking and placing device for culture bottle samples for medical examination
CN113447645B (en) * 2021-06-11 2023-01-31 青岛大学附属医院 Auxiliary taking and placing device for culture bottle samples for medical examination

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20190322

Termination date: 20210326

CF01 Termination of patent right due to non-payment of annual fee