CN1990854A - Microorganism bacterium agent for processing thick oil sewage and preparation method thereof - Google Patents
Microorganism bacterium agent for processing thick oil sewage and preparation method thereof Download PDFInfo
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- CN1990854A CN1990854A CNA2005101309009A CN200510130900A CN1990854A CN 1990854 A CN1990854 A CN 1990854A CN A2005101309009 A CNA2005101309009 A CN A2005101309009A CN 200510130900 A CN200510130900 A CN 200510130900A CN 1990854 A CN1990854 A CN 1990854A
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Abstract
The invention relates to the biological treatment for oil extraction wastewater, which in detail relates to a bacterial agent for treating thick oil wastewater and the method for preparing the same. The comprised components and their proportion by weight are as follows: pseudomonas aeruginosa 5-10%, bacillus subtilis 10-15%, lichen bacilli 5- 15%, Citrobacter propionate tumefaciens 10- 15%, liquid gold tumefaciens 10- 20%, annular gemma bacillus 5- 10%, wilting Bacillus pumilus 5- 10%, spherical arthrobacter 5- 10%, crabstick tumefaciens 5- 10%, and hot ground anaerobic rod baceria 10- 15%. It is prepared by activating bacteria, shake-flask culturing, expanding propagating and mixing. The cooperation action is strong, the biological surface activating agent generating- bacteria can enlarge the dissolution of hydrocarbon petroleum in water and make it is easier for hydrocarbon petroleum to contact with bacteria; the anaerobic bacteria can improve the biodegradation of thick oil wastewater; the degradation perfomace of aerobic bacteria is good and suitable for biological treatment for thick oil wastewater.
Description
Technical field
The present invention relates to the biological treating technology of oil extraction-generated waste water, specifically a kind of compound microbial microbial inoculum and preparation method who handles condensed oil sewage.
Background technology
Along with the development of petroleum industry, the most of oil field of China has all entered high moisture viscous crude or ultra-thick oil thermal is adopted the stage, and water ratio is up to more than 80%～90% in the crude oil extraction liquid.About the processing of oil field extracted water, existing both at home and abroad many reports.Processing to condensed oil sewage mostly is to be back to boiler to do recirculated water after advanced treatment both at home and abroad, and condensed oil sewage is handled and generally comprised technologies such as oily water separation, coagulating sedimentation, air supporting, and its maximum drawback is to satisfy qualified discharge (mainly being that COD is up to standard).External condensed oil sewage fully recovering after advanced treatment is made recirculated water in boiler, does not efflux phenomenon basically.Domestic crude oil water ratio raises gradually, and the reuse amount increases little, and it is serious to cause condensed oil sewage to efflux phenomenon, therefore effluxes the key link that becomes domestic each elephant sewage disposal work up to standard.The essential characteristic of condensed oil sewage is to contain than higher colloid and bituminous matter, and because the singularity of viscous oil recovery technology has added a large amount of polymer medicaments in production engineering, these contaminant molecule amounts are big, biological degradability is poor, and the intractability of sewage is strengthened.In various forms of intermittences of waste water, combination biological treatment, microorganism is in occupation of dominant position, and the biological treatment efficient of condensed oil sewage depends primarily on the biodegradability regulation and control of waste water.But what adopt because of technology such as the film separated activated sludge that adopted, granulating active sludge in the past is indigenous microorganism; can not improve the biodegradability of sewage; and then can not effectively remove pollutent in the condensed oil sewage, solving condensed oil sewage can't qualified discharge also be a difficult problem.
Summary of the invention
The object of the invention provides a kind of processing condensed oil sewage complex micro organism fungicide and preparation method thereof, its microbiobacterial agent synergy is strong, in handling, can improve thick-oil waste water the biodegradability of condensed oil sewage, the hardly degraded organic substance pollutent in the degrade viscous crude oil sewage more effectively solves the difficult problem that condensed oil sewage can't qualified discharge well.
Technical scheme of the present invention is: considering and can handle condensed oil sewage effectively, improve the biodegradability of sewage, solve the difficult problem that condensed oil sewage can't qualified discharge, the present invention mainly is made up of aerobic bacteria, amphimicrobe and anerobe, and each the bacterial classification content in the described microbiobacterial agent can be regulated according to pollutent character in the condensed oil sewage, proportioning.
(weight in wet base) by weight percentage, described microbiobacterial agent composition is: 5～10% pseudomonas aeruginosas, 10～15% subtilises, 5～15% Bacillus licheniformis, 10～15% Fu Shi propionibacteriums, the golden bacillus of 10～20% liquefaction, 5～10% Bacillus circulans, 5～10% wilting bacillus pumilises, 5～10% Arthrobacter globiformis, 5～10% crabstick tumefaciens, 10～15% hot ground anaerobic rod bacerias; Described Arthrobacter globiformis, crabstick tumefaciens are for producing the bio-surfactant bacterium; Pseudomonas aeruginosa, subtilis, Bacillus licheniformis, Fu Shi propionibacterium, the golden bacillus of liquefying, Bacillus circulans, wilting bacillus pumilis, hot ground anaerobic rod baceria are oil degradation bacteria.
Microbiobacterial agent preparation method concrete operations step is:
(1) actication of culture: adopt described bacterial classification, with ordinary method bacterial classification is received on the slant medium in the ready test tube, cultivate respectively, cultivated 3～7 days at 25～30 ℃, wherein hot ground anaerobic rod baceria is cultivated and is carried out under the strictly anaerobic environment;
(2) shake-flask culture: above-mentioned slant culture is made bacteria suspension with sterilized water, and Arthrobacter globiformis, crabstick tumefaciens are inoculated into to produce in the bio-surfactant substratum and cultivate respectively, cultivate 3～7 days at 25～30 ℃; Pseudomonas aeruginosa, subtilis, Bacillus licheniformis, Fu Shi propionibacterium, the golden bacillus of liquefying, Bacillus circulans, wilting bacillus pumilis and hot ground anaerobic rod baceria are inoculated in the bacteria culture medium and cultivate respectively, cultivate 3～7 days at 25～30 ℃; Hot ground anaerobic rod baceria is cultivated and is carried out under the strictly anaerobic environment.
(3) expand numerous: method set by step 2) is carried out;
(4) mix: by described microorganism weight in wet base mixed, the straw powder that adds microbiobacterial agent weight in wet base total amount 20～30% simultaneously is as microbe carrier with the mentioned microorganism fermented liquid.
Described generation bio-surfactant medium component is (gL
-1): ammonium sulfate 5.0g, glucose 2.0g, Repone K 1.10g, sodium-chlor 1.10g, ferrous sulfate 0.028g, potassium primary phosphate 1.5g, dipotassium hydrogen phosphate 1.5g, sal epsom 0.5g, yeast extract paste 0.5g, liquid microelement 5.0ml, diesel oil 20.0ml, pH7～7.5.
Described microbial culture based component is (gL
-1): extractum carnis 5g, peptone 10g, sodium-chlor 5g, pH7～7.5; Described slant medium adopts bacteria culture medium.
Arthrobacter globiformis in the described compound microbial microbial inoculum and crabstick tumefaciens kind belong to generation bio-surfactant bacterial strain, they are matrix with the petroleum hydrocarbon material, synthetic glycolipid compound and surfactant protein etc., these meta-bolitess can reduce the interfacial tension of petroleum hydrocarbon and water, increase the solvability of petroleum hydrocarbon in water, make that petroleum hydrocarbon is easier directly to be contacted with microorganism, promote biological degradation; Described anerobe and amphimicrobe as: hot ground anaerobic rod baceria, Bacillus licheniformis, Fu Shi propionibacterium and Bacillus circulans are converted into macromolecular substance such as petroleum hydrocarbon under anaerobism or anaerobic environment and can be improved the biodegradability of condensed oil sewage by the small-molecule substance of biological utilisation; Described petroleum hydrocarbon degraded mainly is Degradation generation cell paste, the CO by aerobe such as pseudomonas aeruginosa, subtilis, liquefaction gold bacillus and wilting bacillus pumilis
2And H
2O finishes; Owing to producing the coexistence of bio-surfactant bacterium and oil degradation bacteria, the effect when effect ratio of their common performances uses oil degradation bacteria separately improves about 20%; Pollutant component complexity in the sewage because synergy and common metabolism between the microorganism have effectively been brought into play in anaerobic degrading bacteria and the coexistence of aerobic degradation bacteria, improves the processing efficiency of sewage.
During use, complex micro organism fungicide of the present invention being put in the treatment reactor, is carbon source with the petroleum hydrocarbon organic pollutant, reaches the purpose of waste recycling, raising processing efficiency, and non-secondary pollution, and water outlet reaches emission standard.
Advantage of the present invention is:
(1) adopt the mode of multiple bacterial classification combination, taken into account anaerobism, aerobic and amphimicrobe not same-action, improve the biodegradability of condensed oil sewage condensed oil sewage, avoided because of single culture not thorough to sewage disposal.
(2) with oil degradation bacteria and the organic combination of generation tensio-active agent bacterium, effectively utilized microorganism can degrading aliphatic produces bio-surfactant with petroleum hydrocarbon compound such as aromatic series, on microorganism cells surface and interface that hydrocarbon contact characteristic, the solvability of increase pollutent in water.
(3), thereby reduced the cost of sewage disposal because bacterial classification wide material sources of the present invention; Its treatment effect to condensed oil sewage is obvious, easy to operate, so very big practical and popularizing value is arranged.
Description of drawings
Fig. 1 is for adding behind the microbiobacterial agent of the present invention changing conditions from water distribution PetroChina Company Limited. hydrocarbon and family's component concentration thereof.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1
It is (gL that present embodiment adopts the autogamy oily(waste)water
-1): crude oil 10g, ammonium sulfate 0.5g, SODIUMNITRATE 0.5g, calcium chloride 0.02g, sal epsom 0.2g, potassium primary phosphate 1.0g, SODIUM PHOSPHATE, MONOBASIC 1.0g, adjusting pH 7.5, test at the indoor petroleum hydrocarbon degradation that carries out.
Described microbial inoculum adopts bacterial isolates, is specially: 10% pseudomonas aeruginosa (Pseudomonasaeruginosa), 15% subtilis (Bacillus subtilis), 8% Bacillus licheniformis (Bacilluslicheniformis), 12% Fu Shi propionibacterium (Propionibacterium freudennreichii), 14% liquefaction golden bacillus (Aureobacterium liquefaciens), 5% Bacillus circulans (Bacilluscirculans), 10% wilting bacillus pumilis (Curtobacterium flaccumfaciens), 10% Arthrobacter globiformis (Arthrobacter globiformis), 6% crabstick tumefaciens (Clavibacter xyli) and 10% hot ground anaerobic rod baceria (Anaerobaculum thermoterrenum)
Microbiobacterial agent preparation method concrete operations step is:
1) actication of culture: adopt described bacterial classification, with ordinary method bacterial classification inoculation on the slant medium (present embodiment employing bacteria culture medium), was cultivated 5 days at 28 ℃ in the ready test tube, wherein hot ground anaerobic rod baceria is cultivated and is carried out under the strictly anaerobic environment;
2) shake-flask culture: described slant culture is made bacteria suspension with sterilized water, Arthrobacter globiformis, crabstick tumefaciens are inoculated into to produce in the bio-surfactant substratum and cultivated 5 days at 28 ℃ respectively, pseudomonas aeruginosa, subtilis, Bacillus licheniformis, Fu Shi propionibacterium, the golden bacillus of liquefying, Bacillus circulans, wilting bacillus pumilis, hot ground anaerobic rod baceria are inoculated in the bacteria culture medium and cultivate respectively, cultivated 5 days at 28 ℃, hot ground anaerobic rod baceria is cultivated and is carried out under the strictly anaerobic environment.
3) expand numerous: method set by step 2) is carried out;
4) mix: the microbial fermentation solution with described expansion after numerous is by described microorganism weight in wet base mixed, mixing microorganisms microbial inoculum 150.0g, add straw powder 30.0g simultaneously as microbe carrier.
Described generation bio-surfactant medium component is (gL
-1): ammonium sulfate 5.0g, glucose 2.0g, Repone K 1.1g, sodium-chlor 1.1g, ferrous sulfate 0.028g, potassium primary phosphate 1.5g, dipotassium hydrogen phosphate 1.5g, sal epsom 0.5g, yeast extract paste 0.5g, liquid microelement 5ml, diesel oil 20ml, pH are 7.
Described microbial culture based component is (gL
-1): extractum carnis 5g, peptone 10g, sodium-chlor 5g, pH7.2.
Degradation treatment:
The autogamy oily(waste)water sterilized under 1 normal atmosphere to add behind the 30min in the reactor, the high 21cm of reactor, and cubic capacity 15L, the right cylinder device of selecting for use the PVC material to make is furnished with the aeration-agitation device in the reactor.
The microbiobacterial agent for preparing is inoculated in the autogamy oily(waste)water, and inoculum size is 1%, 28 ℃ of cultivation of oily(waste)water weight, and at preceding 7 days that cultivate, every day, continuous aeration-agitation was 8 hours; At back 8 days that cultivate, ventilated continuously every day 16 hours.Cultivating sampling in the 3rd, 6,9,12 and 15 day, measure variation respectively from total amount and each family's component of water distribution PetroChina Company Limited. hydrocarbon.
The present invention is according to the different characteristics of every kind of bacterial strain, different purposes, made full use of the different qualities of each bacterial strain, bio-surfactant produces bacterium can increase the solvability of petroleum hydrocarbon in water, make that petroleum hydrocarbon is easier directly to be contacted with microorganism, anerobe can be improved the biodegradability of thick-oil waste water, the aerobic bacteria degradation capability is big, and its synergy is strong, is applicable to the biological treatment of condensed oil sewage.From measurement result: after adding microbiobacterial agent, the content of waste water PetroChina Company Limited. hydrocarbon sharply reduces, and through 15 days processing, the clearance of total hydrocarbon reached 57.25%; Under microbial process of the present invention, also great changes will take place in treating processes for each family's component, the content of aromatic hydrocarbons at first raises and reduces then, colloid, bitum content at first reduce the back and raise, explanation is under action of microorganisms, but the conversion of part macromolecular substance has improved the biodegradability of waste water for the small-molecule substance of biological utilisation in the pollutent, thereby improved the clearance of petroleum hydrocarbon, as shown in Figure 1.
Embodiment 2
Difference from Example 1 is:
Experimental water is the Liaohe Oil Field dense oil extraction wastewater, the composition complexity, and wherein organic pollutant is mainly alkane, aromatic hydrocarbons, organic acid and oil colloid, and unsaturated hydro carbons and non-hydrocarbons substances content are lower.The following material proportion of 12 carbon is not high in the stable hydrocarbon, and high carbon chain and aromatic substance ratio are higher, and sewage is difficult to degraded, and the mean value of CODcr is about 300mg/L.
Described microbial inoculum adopts bacterial isolates, is specially: 8% pseudomonas aeruginosa (Pseudomonasaeruginosa), 12% subtilis (Bacillus subtilis), 5% Bacillus licheniformis (Bacilluslicheniformis), 10% Fu Shi propionibacterium (Propionibacterium freudennreichii), 20% liquefaction golden bacillus (Aureobacterium liquefaciens), 10% Bacillus circulans (Bacilluscirculans), 5% wilting bacillus pumilis (Curtobacterium flaccumfaciens), 5% Arthrobacter globiformis (Arthrobacter globiformis), 10% crabstick tumefaciens (Clavibacter xyli) and 15% hot ground anaerobic rod baceria (Anaerobaculum thermoterrenum).
Microbiobacterial agent preparation method concrete operations step is:
1) actication of culture: adopt described bacterial classification, with ordinary method bacterial classification is received on the slant medium in the ready test tube (with embodiment 1), cultivated 3 days at 30 ℃, wherein hot ground anaerobic rod baceria is cultivated and is carried out under the strictly anaerobic environment;
2) shake-flask culture: described slant culture is made bacteria suspension with sterilized water, Arthrobacter globiformis, crabstick tumefaciens are inoculated into to produce in the bio-surfactant substratum and cultivated 3 days at 30 ℃ respectively, be inoculated on pseudomonas aeruginosa, subtilis, Bacillus licheniformis, Fu Shi propionibacterium, the golden bacillus of liquefying, Bacillus circulans, wilting bacillus pumilis, the hot ground anaerobic rod baceria inclined-plane in the bacteria culture medium and cultivate respectively, cultivated 3 days at 30 ℃, hot ground anaerobic rod baceria is cultivated and is carried out under the strictly anaerobic environment.
3) expand numerous: method set by step 2) is carried out;
4) mix: the microbial fermentation solution with described expansion after numerous is by described microorganism weight in wet base mixed, mixing microorganisms microbial inoculum 5000g, add straw powder 1500g simultaneously as microbe carrier.
Degradation treatment:
The microbiobacterial agent for preparing is added in the bio-reactor that condensed oil sewage is handled, and dosage is 1% of an oily(waste)water weight, and reactor is the anaerobic-aerobic integrated bioreactor, and its hydraulic detention time is 8 hours.Adopt gas chromatography-mass spectrography (GC/MS) analytical test to add microbial inoculum after stain thing of the present invention in anaerobic reaction variation wherein; Adopt potassium dichromate process to measure the CODcr concentration of sewage water inlet, anaerobism water outlet, outputted aerobic water.
Find through 3 months monitorings: all below 100mg, the CODcr average removal rate is about 75% to the water outlet CODcr after the processing, does not compare raising about 25% than do not add microbiobacterial agent CODcr clearance 50% of the present invention in the past, sees attached list 1;
Subordinate list 1 adds the variation (mgL of CODcr in the microbial inoculum post-reactor of the present invention
-1)
| Time | Water inlet CODcr | Anaerobism water outlet CODcr | Outputted aerobic water CODcr |
| 2004.5.7 | 328.24 | 183.28 | 79.16 |
| 2004.6.7 | 309.69 | 173.11 | 80.33 |
| 2004.7.7 | 294.35 | 160.48 | 75.39 |
| 2004.8.8 | 317.12 | 165.99 | 77.98 |
After anaerobic treatment, organic matter molecular mass obviously reduces, and 100～200 parts account for 30%, accounts for 40% greater than 300 parts.Showing as alcohols material on compound is formed has a certain proportion of increase, and the aldehyde material showed increased is not carried out fully though anaerobic reaction is described, the anaerobism degree is very big.The colloid material that records before the anaerobism all disappears, and the aromaticity content ratio increases in the unsaturated materials, sees attached list 2.
Subordinate list 2 is handled front and back pollutent variation after adding microbial inoculum of the present invention in anaerobic reactor
| Project | After the former water treatment | Project | After the former water treatment |
| The organism kinds number | 67 57 | The hydrocarbon of＞C26 | 9 8 |
| The organism number that contains C-H-O | 32 33 | The organic content of C-H-O, mgL -1 | 40.86 8.78 |
| Wherein: phenols | 17 17 | Wherein: phenols | 37.93 5.45 |
| Ketone | 3 3 | Ketone | 0.389 0.11 |
| The ester class | 4 4 | The ester class | 1.467 2.85 |
| Acids | 3 3 | Acids | 0.195 0.16 |
| Alcohols | 4 5 | Alcohols | 0.619 0.19 |
| Quinones | 1 1 | Quinones | 0.260 0.02 |
| The organism number that contains C-H | 35 24 | The organic content of C-H, mgL -1 | 23.06 2.21 |
| Wherein:＜C15 | 11 6 | The organic content that is detected, mgL -1 | 63.92 10.99 |
| The hydrocarbon of C16～C25 | 15 10 | - | - - |
Embodiment 3
Difference from Example 1 is:
Experimental water is a Liaohe Oil Field sewage work condensed oil sewage, this sewage quality meta-alkalescence, and after oil removal, air supporting were handled, CODcr concentration was 200～220mgL
-1, the BOD5 value is very low, and BOD5/CODcr does not have biodegradability substantially less than 0.10.Organic polymer such as petroleum-type and emulsifying agent, emulsion splitter is principal pollutant, also is main difficult degradation pollutent.
Described microbial inoculum adopts bacterial isolates, is specially: 5% pseudomonas aeruginosa (Pseudomonasaeruginosa), 10% subtilis (Bacillus subtilis), 15% Bacillus licheniformis (Bacillus lichenformis), 15% Fu Shi propionibacterium (Propionibacteriumfreudennreichii), 10% liquefaction golden bacillus (Aureobacterium liquefaciens), 9% Bacillus circulans (Bacillus circulans), 9% wilting bacillus pumilis (Curtobacteriumflaccumfaciens), 9% Arthrobacter globiformis (Arthrobacter globiformis), 5% crabstick tumefaciens (Clavibacter xyli) and 13% hot ground anaerobic rod baceria (Anaerobaculum thermoterrenum).
Microbiobacterial agent preparation method concrete operations step is:
1) actication of culture: adopt described bacterial classification, with ordinary method bacterial classification is received on the slant medium in the ready test tube (with embodiment 1), cultivated 7 days at 25 ℃, wherein hot ground anaerobic rod baceria is cultivated and is carried out under the strictly anaerobic environment;
2) shake-flask culture: described slant culture is made bacteria suspension with sterilized water, Arthrobacter globiformis, crabstick tumefaciens are inoculated into to produce in the bio-surfactant substratum and cultivated 7 days at 25 ℃ respectively, be inoculated on pseudomonas aeruginosa, subtilis, Bacillus licheniformis, Fu Shi propionibacterium, the golden bacillus of liquefying, Bacillus circulans, wilting bacillus pumilis, the hot ground anaerobic rod baceria inclined-plane in the bacteria culture medium and cultivate respectively, cultivated 7 days at 25 ℃, hot ground anaerobic rod baceria is cultivated and is carried out under the strictly anaerobic environment.
3) expand numerous: method set by step 2) is carried out;
4) mix: the microbial fermentation solution with described expansion after numerous is by described microorganism weight in wet base mixed, mixing microorganisms microbial inoculum 8000g, add straw powder 2000g simultaneously as microbe carrier.
Degradation treatment:
The microbiobacterial agent for preparing is added in the bio-reactor that condensed oil sewage is handled, and dosage is 1% of an oily(waste)water weight, and reactor is the anaerobic-aerobic integrated bioreactor, and its hydraulic detention time is 24 hours.Adopt gas chromatography-mass spectrography (GC/MS) analytical test to add microbial inoculum after stain thing of the present invention in anaerobic reaction variation wherein; Adopt potassium dichromate process to measure water inlet, water outlet CODcr concentration.
Through after the anaerobic biological treatment, most of larger molecular organics is converted into biodegradable small molecules organic compound in the water, when water inlet CODcr concentration at 200～220mgL
-1The time, water outlet CODcr concentration is at 60～70mgL
-1, the CODcr clearance can reach more than 70%.Subordinate list 3 is to mainly contain organic pollutants analyzing and testing result before and after biological treatment.
Mainly containing organic pollutants in the water before and after subordinate list 3 biological treatments changes
| Organic pollutant before the biological treatment | Organic pollutant in the water after the anaerobic treatment | |||||||
| Ratio % | Molecular formula | Molecular weight | Ratio % | Molecular formula | Molecular weight | Ratio % | Molecular formula | Molecular weight |
| 2.51 1.32 9.43 8.49 2.41 1.42 6.87 1.03 1.01 24.58 7.49 3.2 3.92 | C 24H 38O 4 C 18H 31N C 16H 32O 2 C 22H 38O 2 C 19H 40 C 23H 28O 7 C 15H 30O 3 C 13H 24O 2 C 21H 24N 2O 6 C 15H 28O C 18H 38O C 43H 88 C 29H 52O 2 | 390 261 256 322 254 416 258 212 400 224 270 604 432 | 13.35 4.24 4.08 2.08 3.61 3.44 1.89 2.16 2.95 14.10 2.47 1.99 2.64 | C 7H 10O C 9H 16 C 8H 12O C 19H 40 C 8H 10O C 9H 12O C 15H 30O 2 C 18H 38 C 21H 22O 4 C 16H 22O 4 C 21H 44 C 19H 38O 2 C 13H 28 | 110 124 124 268 122 136 242 268 268 334 296 298 184 | 2.97 2.63 1.04 2.22 9.01 10.05 1.84 1.34 1.04 2.97 2.63 1.04 2.22 | C 13H 38 C 21H 44 C 23H 46 C 21H 44O C 24H 38O 4 C 24H 38O 4 C 43H 88 C 18H 38 C 20H 40O 2 C 13H 38 C 21H 44 C 23H 46 C 21H 44O | 254 296 322 296 390 390 604 254 312 254 296 322 296 |
Claims (3)
1, a kind of microbiobacterial agent of handling condensed oil sewage, it is characterized in that: by weight percentage, described microbiobacterial agent composition is: 5～10% pseudomonas aeruginosas, 10～15% subtilises, 5～15% Bacillus licheniformis, 10～15% Fu Shi propionibacteriums, the golden bacillus of 10～20% liquefaction, 5～10% Bacillus circulans, 5～10% wilting bacillus pumilises, 5～10% Arthrobacter globiformis, 5～10% crabstick tumefaciens, 10～15% hot ground anaerobic rod bacerias; Described weight is weight in wet base.
2, a kind of microbiobacterial agent preparation method according to the described processing condensed oil sewage of claim 1 is characterized in that the concrete operations step is:
1) actication of culture: adopt pseudomonas aeruginosa, subtilis, Bacillus licheniformis, Fu Shi propionibacterium, the golden bacillus of liquefying, Bacillus circulans, wilting bacillus pumilis, hot ground anaerobic rod baceria, Arthrobacter globiformis, crabstick tumefaciens bacterial classification, on the slant medium of described bacterial classification inoculation in the test tube, cultivated respectively 3～7 days at 25～30 ℃;
2) shake-flask culture: above-mentioned slant culture is made bacteria suspension with sterilized water, Arthrobacter globiformis, crabstick tumefaciens are inoculated into to produce in the bio-surfactant substratum again and cultivate respectively, cultivated 3～7 days down at 25～30 ℃; Pseudomonas aeruginosa, subtilis, Bacillus licheniformis, Fu Shi propionibacterium, the golden bacillus of liquefying, Bacillus circulans, wilting bacillus pumilis, hot ground anaerobic rod baceria are inoculated in the bacteria culture medium and cultivate respectively, cultivate 3～7 days down at 25～30 ℃;
3) expand numerous: method set by step 2) is carried out;
4) mix: by described microorganism weight in wet base mixed, the straw powder that adds microbiobacterial agent weight in wet base total amount 20～30% simultaneously gets microbiobacterial agent as microbe carrier with the described microbial fermentation solution of step 3).
3, according to the microbiobacterial agent preparation method of the described processing condensed oil sewage of claim 2, it is characterized in that: step 1), 2 wherein) and 3) described hot ground anaerobic rod baceria cultivates and carry out under anaerobic environment.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005101309009A CN1990854A (en) | 2005-12-27 | 2005-12-27 | Microorganism bacterium agent for processing thick oil sewage and preparation method thereof |
| PCT/CN2006/002675 WO2007073656A1 (en) | 2005-12-27 | 2006-10-12 | A microbial preparation for treating thick oil sewage and method for preparing the same |
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| CNA2005101309009A CN1990854A (en) | 2005-12-27 | 2005-12-27 | Microorganism bacterium agent for processing thick oil sewage and preparation method thereof |
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| CN1990854A true CN1990854A (en) | 2007-07-04 |
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