CN1789406A - Microbe bacteria agent for treatment of refinery waste water, its preparation method and application - Google Patents
Microbe bacteria agent for treatment of refinery waste water, its preparation method and application Download PDFInfo
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- CN1789406A CN1789406A CN 200410081504 CN200410081504A CN1789406A CN 1789406 A CN1789406 A CN 1789406A CN 200410081504 CN200410081504 CN 200410081504 CN 200410081504 A CN200410081504 A CN 200410081504A CN 1789406 A CN1789406 A CN 1789406A
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Abstract
The invention discloses a microbiological bacterium for processing waste water in oil refining, its preparing process and use. The bacterium comprises Rhodococcus, Microbacterium spp, bacillus, Candida and Brachybacterium by the ratio of 0-10:0-10:0-10:0-10:0-10:0-10. The bacterium can be activated for treating waste water.
Description
Technical field
The invention belongs to the environmental protection field, relate to a kind of microbiobacterial agent, its preparation method and application thereof, be specifically related to according to the microbial ecology principle, interaction relationships such as the symbiosis between the multiple efficient degrading bacteria of difficult degradation compounds such as utilization degraded alkanes, arene, substituted benzene, phenols, alternate, synergy are built into the preparation method and the using method of microbiobacterial agent and this microbial inoculum of Refinery Wastewater.
Background technology
Refinery water belongs to high concentration hard-degraded organic waste water, quantity discharged is big, mainly contain pollutents such as alkanes, arene, volatile phenol and ammonia nitrogen, because toxicity of compound such as alkanes, arene, volatile phenol are very big, discharging contains the high waste water of these substances content and will cause great pollution to environment, need handle and could discharge.At present, the waste water of domestic refinery mostly adopts the method for oil removal-air supporting-biochemical treatment to handle, and wherein biochemical treatment mainly is to adopt various activated sludge treatment methods, and processing efficiency is not high, and by the hand active sludge form complicated, change big, difficult control; In addition, can reach comprehensive discharge standard even discover waste water both at home and abroad through oil removal-air supporting-biochemical treatment, but still include solvability aliphatic hydrocarbon, benzene series thing, sulfocompound and phthalate compound etc. in the water outlet, wherein multiple compound belongs to priority pollutants in China's water, be disposed in the surrounding environment and can work the mischief, therefore be necessary to change treatment process these toxic compounds are handled to reduce the harm that it causes surrounding environment.
Can obtain degraded to the special compound in the waste water by separating the biological reinforcing technology that efficient degrading bacteria is inoculated in the biochemical reaction structures, but this method and be not suitable for the refinery water that contains multiple difficult degradation compound.Microbial inoculum is a kind of mixed bacterium culture system, can utilize wherein multiple microorganism independent or multiple between degrade the efficiently pollutent of various complexity of synergy.Actual needs by different contaminate environment; The various types of degradation bacteria of seed selection are formed the microorganism species that is applicable to different contaminate environment, through the fermentation enlarged culturing, make microbiobacterial agent, on purpose are added in all kinds of biochemical reaction structures, can reach the purpose of improving processing efficiency.
Summary of the invention
Through still including the shortcoming of priority pollutants in China's water such as solvability aliphatic hydrocarbon, benzene series thing, sulfocompound and phthalate compound in the water outlet after oil removal-air supporting-biochemical treatment, the purpose of this invention is to provide a kind of microbiobacterial agent, its preparation method and application thereof of Refinery Wastewater at refinery water.
The present invention can realize in the following manner:
Microbiobacterial agent among the present invention comprises bacterial classification, substratum, sorbent material.
Bacterial classification comprises one or more microorganism strains of following ratio:
14357(CGMCC NO:1262): 0-10
Na4(CGMCC NO:1270): 0-10
EM(CGMCC NO:0868): 0-10
CB4(CGMCC NO:1267): 0-10
PNCB3(CGMCC NO:1271): 0-10
10-4(CGMCC NO:1268): 0-10
Wherein, bacterial strain Na4 is Rhod Rhosococcus, and preserving number is CGMCC NO:1270, the naphthalene of mainly degrading; Bacterial strain PNCB3 is small Bacillaceae Exiguobacterium, and preserving number is CGMCC NO:1271, the parachloronitrobenzene of mainly degrading; Bacterial strain CB4 is genus bacillus bacillus, and preserving number is CGMCC NO:1267, the monochloro-benzene of mainly degrading; Bacterial strain 10-4 is candiyeast candida, and preserving number is CGMCC NO:1268, main degradation of phenol; Bacterial strain 14357 is short shape bacillus Brachybacterium collins, and preserving number is CGMCC NO:1262, the alkane of mainly degrading.
Above bacterial strain is submitted China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on December 8th, 2004.
Bacterial strain EM is preservation strain, and preserving number is CGMCC NO:0868, the luxuriant and rich with fragrance pyrene of the anthracene of mainly degrading.
It is extractum carnis 0.1%-1%, peptone 0.1%-1%, NaCl 0.1%-1%, water 100g that the culture medium prescription of described microbiobacterial agent can be used substratum, screening formulation with the common micro-organisms cultivation, and solid medium adds agar 1.0-2%.
Described sorbent material can adopt the material through the tool adsorptive liquid of sterilising treatment, is preferably the dry wheat bran through sterilising treatment.
The preparation method of microbiobacterial agent is among the present invention:
(1) waste water that need are handled carries out composition analysis, selects bacterial strain and the ratio that suits and be seeded in the sterilization solid medium activation respectively standby according to the result of pollutant component analysis;
(2) substratum that sterilising treatment is crossed is quantitatively standby;
(3) bacterial classification after will activating is seeded in the substratum that sterilising treatment crosses shaking culture 1-5 days respectively;
(4) with cultured each bacterium liquid numeration, again by aforementioned quantitative proportion make up mixed bacteria liquid;
(5) the wheat bran drying of high-temperature sterilization being handled is quantitatively standby;
(6) will contain in the quantitative mixed bacteria liquid adding sorbent material of substratum, again the package cryopreservation.
The mixed bacteria liquid add-on is the following any amount of the saturated absorption of sorbent material, and optimal addn is: add the 1L substratum in the 1Kg sorbent material.
By the microbiobacterial agent of above-mentioned preparation add in use P contained compound as auxiliary agent to replenish phosphorus, be preferably KH
2PO
4
The auxiliary agent usage quantity is: 1% of the total COD value of refinery water.
According to above-mentioned inoculating proportion that provides and production method, the product leading indicator of its preparation is:
Viable count is greater than 1,000,000,000/g; PH=6-7
The using method of microbial inoculum is among the present invention: after becoming the microbial inoculum of component selections suitable proportion bacterial strain composition to adopt aforesaid method to make the microbial inoculum product according to the waste water of predicting, unpacking adds to microbial inoculum in the wastewater treatment structures, adds the auxiliary agent of respective amount simultaneously.At refinery water COD scope be that 0-3000mg/l, pH scope are 5-9.
The present invention has the following advantages:
(1) comprised and to degrade multiple biodegradable organic compounds efficient degradation bacterial classification and realize effective combination of various efficient degradation bacterial classifications;
(2) improve the method for preserving of microbial inoculum, prolonged product work-ing life;
(3) the refinery water processing efficiency can be improved, and solvability aliphatic hydrocarbon, benzene series thing and the polycyclic arene compound of the multiple difficult degradation that present oil removal-air supporting-biochemical treatment can not remove can be removed;
(4) easy to use, packing, transportation facility form commercialization.
Embodiment
Embodiment 1:
1 measures the various physical and chemical indexs of waste water, and the COD value adopts potassium dichromate process to measure, oil-containing value employing gravimetric determination, and volatile phenol content adopts distillation back 4-aminoantipyrene spectrophotometry, and organism is formed employing GC-MS analytical procedure and is measured.The waste water quality analytical results is as follows:
Classification | COD | Oleaginousness | Volatile phenol | ||
Content (mg/l) | 1590 | 101 | 37.74 | ||
Classification | Alkanes | Contain benzene ring compound | |||
Organism relative content (%) | 64.18 | 35.82 |
2 is quantitatively standby with high-temperature sterilization processing (121 ℃, 30 minutes) solid medium excessively, and the prescription of solid medium is extractum carnis 0.5%, peptone 1%, NaCl 0.5%, water 100g, agar 1.7%.
3 prefabricated bacterial classifications and will be prefabricated 14357, Na4, EM, CB4, PNCB3,10-4 be seeded to activate in the sterilization solid medium standby.
4 high-temperature sterilization to be handled the liquid nutrient medium of (121 ℃, 30 minutes) quantitatively standby, and the liquid culture based formulas is extractum carnis 0.5%, peptone 1%, NaCl 0.5%, water 100g.
5 bacterial classifications after will activating are seeded in the liquid nutrient medium vibration (37 ℃, 160 rev/mins) respectively and cultivated 2 days.
6 with the numeration of cultured bacterium liquid, again by 14357: Na4: CB1: PNCB3: 10-4: EM=3: 4: 2: 3: 3: 6 quantitative proportion make up mixed bacteria liquid.
8 using method: in the Erlenmeyer flask that 100ml sterilization refinery water is housed, add the 5ml active sludge, add the thalline that removes substratum and the KH of microbial inoculum 10ml process centrifugal (5000 rev/mins) the back gained that is mixed with again
2PO
41.5mg, 3 of parallel laboratory tests, 30 ℃, 160 rev/mins of oscillatory reactions are done simultaneously and are not added mud and do not add bacteria suspension and do not add KH
2PO
4Blank, only add the contrast of mud.COD, result such as the following table of the centrifugal mensuration supernatant liquor of sampling after 3 days
Show: reach with microbial inoculum and compare with the treatment effect of microbial inoculum to refinery water
Classification | Contrast | Mud | Microbial inoculum+mud |
Initial COD (mg/l) water outlet COD (mg/l) degradation rate (%) | 1526 1075 29.53 | 1528 733.7 51.97 | 1561 86 94.51 |
As seen from the above table, mud is 51.97% to the treatment effect of refinery water during not with microbial inoculum, reaches 94.51% with degradation rate after the microbial inoculum, and the processing efficiency ratio did not improve 42.52% with the treatment effect of microbial inoculum after microbial inoculum added in the mud
Embodiment 2:
1 waste water preliminary survey and mixed bacteria liquid prepare with embodiment 1,
2 will make microbial inoculum in quantitative mixed bacteria liquid (containing substratum) the adding sorbent material, and add-on is: add 1L substratum, package cryopreservation again in the 1Kg sorbent material.
3 using method: in the sbr reactor device, add active sludge by 10% add-on, the minimal medium that adds the glucose that contains 1000mg/l carries out culturing sludge, adopt the mode of intermittently changing water, change water every day 2 times, cultivate that mud 30min rate of descent reaches 10% after 7 days, in water inlet, increase the proportion of refinery water more gradually, make the microorganism in the mud under the new living condition that adapts to gradually, obtain domestication, till water inlet all is refinery water, in system for handling, add the KH of microbial inoculum and the total COD value 1% of refinery water again by 10% inoculum size
2PO
4, do the control experiment that does not add microbial inoculum simultaneously, vexed exposing to the sun 3 days, the COD value of mensuration water inlet and water outlet after 3 days,
Result such as following table.
Experimental result
COD (mg/l) time (hour) | 0 | 3h | 6h | 9h |
The contrast microbial inoculum | 937 979 | 636 578 | 452 329 | 266 100 |
As seen from the above table, mud is 71.61% to the treatment effect of refinery water when handling after 9 hours not with microbial inoculum, reaches 89.79% with degradation rate after the microbial inoculum, and processing efficiency improved 25.39% than the processing efficiency with microbial inoculum after microbial inoculum added in the mud.
Embodiment 3:
The various physical and chemical index measuring methods of 1 waste water are with embodiment 1.The result is as follows:
Classification | COD | Oleaginousness | Volatile phenol |
Content (mg/l) | 963 | 22 | 126.5 |
Classification | Alkanes | Contain benzene ring compound |
Organism relative content (%) | 13.67 | 86.33 |
2 is quantitatively standby with high-temperature sterilization processing (121 ℃, 30 minutes) solid medium excessively, and the prescription of solid medium is extractum carnis 0.3%, peptone 1%, NaCl 0.5%, water 100g, agar 1.7%.
3 prefabricated bacterial classifications and will be prefabricated 14357, EM, PNCB3,10-4 be seeded to activate in the sterilization solid medium standby.
4 high-temperature sterilization to be handled the liquid nutrient medium of (121 ℃, 30 minutes) quantitatively standby, and the liquid culture based formulas is extractum carnis 0.3%, peptone 1%, NaCl 0.5%, water 100g.
5 bacterial classifications after will activating are seeded in the liquid nutrient medium vibration (37 ℃, 160 rev/mins) respectively and cultivated 3 days.
6 with the numeration of cultured bacterium liquid, again by 14357: PNCB3: 10-4: EM=2: 1: 10: 3 quantitative proportion make up mixed bacteria liquid.
7 with in quantitative mixed bacteria liquid (containing substratum) the adding sorbent material, and add-on is: add 0.5L substratum, package cryopreservation again in the 1Kg sorbent material.
8 using method: the KH that in the contact oxidation method reactor, adds microbial inoculum and the total COD value 1% of refinery water by 10% add-on
2PO
4Vexed exposing to the sun 3 days, adopt the mode of intermittently changing water then, change water every day 2 times, increase the proportion of refinery water in the water inlet gradually, make the microorganism in the microbial inoculum under the new living condition that adapts to gradually, obtain domestication, till water inlet all is refinery water, the COD value of water inlet and water outlet is measured in two all backs, does simultaneously not add the control experiment that microbial inoculum only adds mud.Result such as following table
COD (mg/l) time (hour) | 0 | 3h | 6h | 9h |
The contrast microbial inoculum | 627 633 | 455 388 | 343 156 | 215 84 |
As seen from the above table, handling mud after 9 hours is 66.71% to the treatment effect of refinery water, reaches 86.73% with degradation rate after the microbial inoculum, and processing efficiency improved 31.51% than the processing efficiency with microbial inoculum after microbial inoculum added in the mud.
Claims (6)
1, a kind of microbiobacterial agent of Refinery Wastewater, comprising ratio is the Rhod Rhosococcus of 0-10: 0-10: 0-10: 0-10: 0-10: 0-10, preserving number is CGMCC NO:1270; , small Bacillaceae Exiguobacterium, preserving number are CGMCC NO:1271; Genus bacillus bacillus, preserving number are CGMCC NO:1267; Candiyeast candida, preserving number are CGMCC NO:1268; Short shape bacillus Brachybacterium collins, preserving number is that CGMCC NO:1262 and preserving number are CGMCCNO:0868, and the preservation place is China Committee for Culture Collection of Microorganisms common micro-organisms center, and preservation date is on December 8th, 2004.
2, the ratio of the microbiobacterial agent of the described Refinery Wastewater of claim 1 becomes component selections according to the waste water of need processing.
3, the cultural method of the microbiobacterial agent of the described Refinery Wastewater of claim 1 comprise inoculation, shaking culture, enumeration, be mixed in proportion, preservation, cultivate the substratum that uses and be common micro-organisms cultivation substratum, screening formulation is extractum carnis 0.1%-1%, peptone 0.1%-1%, NaCl 0.1%-1%, water 100g, and solid medium adds agar 1.0-2%.
4, in the cultural method of the microbiobacterial agent of the described Refinery Wastewater of claim 3, can use material through the tool adsorptive liquid of sterilising treatment as sorbent material, the mixed bacteria liquid add-on is the following any amount of the saturated absorption of sorbent material, and optimal addn is: add the 1L substratum in the 1Kg sorbent material.
5, the using method of the microbiobacterial agent of the described Refinery Wastewater of claim 1: after the microbial inoculum that becomes component selections suitable proportion bacterial strain to form according to the waste water of prediction adopts aforesaid method to make the microbial inoculum product, unpacking adds to microbial inoculum in the wastewater treatment structures, adds the auxiliary agent of respective amount simultaneously.At refinery water COD scope be that 0-3000mg/l, pH scope are 5-9.
6, the auxiliary agent that uses in the claim 5 is P contained compound, is preferably KH
2PO
4
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