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CN1785153B - Nano-grade material preparation apparatus, and method for preparing nano-anti-oxidation active poly peptide medicine - Google Patents

Nano-grade material preparation apparatus, and method for preparing nano-anti-oxidation active poly peptide medicine Download PDF

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Publication number
CN1785153B
CN1785153B CN 200510045515 CN200510045515A CN1785153B CN 1785153 B CN1785153 B CN 1785153B CN 200510045515 CN200510045515 CN 200510045515 CN 200510045515 A CN200510045515 A CN 200510045515A CN 1785153 B CN1785153 B CN 1785153B
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China
Prior art keywords
operating room
poly peptide
active poly
peptide medicine
nano
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Expired - Fee Related
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CN 200510045515
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Chinese (zh)
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CN1785153A (en
Inventor
谭金山
王春波
孙谧
姚如永
戴礼森
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Qingdao University
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Qingdao University
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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)

Abstract

An apparatus for preparing the nano-class material is composed of raw material tank, working chamber, vacuumizing system, spinning-beating hollow disc with 120 beating heads in it, and high-speed motor driver system. A process for preparing the nano-class antioxidizing polypeptide medicine includes such steps as loading active polyepetide medicine in the raw material tank, immersing it along withworking chamber in liquefided N2 for 5-10 min, vacuumizing, opening the valve for dropping the medicine into working chamber, starting the spinning-beating hollow disc to rotor at 28000-32000 rpm for1-1.5m, and negative-pressure discharging the nano-class medicine.

Description

The preparation method of nanometer materials preparation facilities and nano-anti-oxidation active poly peptide medicine
Technical field
The invention belongs to pharmacy and field of nanometer technology, more particularly relate to the design of the preparation method of nanometer materials preparation facilities and nano-anti-oxidation active poly peptide medicine.
Background technology
The preparation method of tradition nanometer polypeptide medicine mostly is chemical method greatly, and its major defect is that polypeptide drugs lose activity.In addition, its preparation technology's long flow path and complexity, cost is also high.
The Mechanical Method for preparing fine polypeptide drugs before is difficult to reach nanoscale, and at present best fine polypeptide drugs granule is also greater than 1um.The temperature rise of Mechanical Method has destroyed biological activity.The clinical experience explanation, the curative effect of this um grade particles is relatively poor.
Summary of the invention
Purpose of the present invention just is to overcome above-mentioned shortcoming and defect, and the preparation method of a kind of Mechanical Method nanometer materials preparation facilities and nano-anti-oxidation active poly peptide medicine is provided.It can prepare anti-oxidation active poly peptide medicine and other nanometer materials of nano-scale.In the material of preparation<the reaching more than 95% of 100nm.Preparation technology is simple, and flow process is short, and cost is also low.Clinical efficacy improves greatly.
In order to achieve the above object, nanometer materials preparation facilities of the present invention comprises the storage tank, the operating room that is connected with bottom valve that have seal cover and bottom valve, is installed in pumped vacuum systems in storage tank and the operating room, is installed in that to have in the operating room that revolving of driving shaft hit dish, be installed in rotating speed on the driving shaft be 28000~32000 rev/mins motor driven systems.Revolve hit dish periphery uniform impact head more than 120, top, operating room has sealable discharging opening, should seal between driving shaft and the operating room.
The preparation method of nano-anti-oxidation active poly peptide medicine may further comprise the steps:
(1) active poly peptide medicine is placed storage tank, cover seal cover, simultaneously the discharging opening of operating room is built sealing;
(2) storage tank and operating room were immersed in the liquid nitrogen 5~10 minutes together with active poly peptide medicine;
(3) just storage tank and operating room's evacuation and maintenance;
(4) open bottom valve, make active poly peptide medicine fall into the operating room, close bottom valve then;
(5) actuating motor revolves the motor high-speed driving to hit dish to impact active poly peptide medicine 1~1.5 minute, and revolving the rotating speed that hits dish is 28000~32000 rev/mins;
(6) open the discharging opening of operating room, the active poly peptide medicine after will processing with negative pressure subsequently takes out, and preparation finishes.
Wherein the vacuum of step (3) should remain (5~7) * 0.01pa.
Revolve that to hit the linear velocity that dish impacts an outer end be 400~500m/s.
Task of the present invention comes to this and finishes.
Preparation facilities of the present invention can prepare anti-oxidation active poly peptide medicine and other nanometer materials of nano-scale.In the material of preparation<the reaching more than 95% of 100nm.Preparation technology is simple, and flow process is short, and cost is also low, temperature rise is low, does not destroy biological activity.It can be widely used in the preparation of various nanometer materials.Nano-anti-oxidation active poly peptide medicine can be widely used in the various treating for skin disease, and clinical efficacy improves greatly.
Description of drawings
Figure 1 shows that the polypeptide drugs powder granule that preparation is preceding, granule is between 10-100um.
Figure 2 shows that one of intermediate products in the preparation process, granule is between 1-10um.
Figure 3 shows that two of intermediate products in the preparation process, granule is less than 1um.
Fig. 4 is the particulate SEM photo of preparation back nano-anti-oxidation active poly peptide medicine, and granule is less than 100nm.
Fig. 5 is the particulate TEM photo of preparation back nano-anti-oxidation active poly peptide medicine, and granule is less than 100nm.
Fig. 6 is the schematic diagram of preparation facilities.
The specific embodiment
Embodiment 1.A kind of nanometer materials preparation facilities, as shown in Figure 6.It comprises the storage tank 3, the operating room 4 that is connected with bottom valve 2 that have seal cover 1 and bottom valve 2, be installed in pumped vacuum systems 5 in storage tank 3 and the operating room 4, be installed in and have revolving of driving shaft 6 in the operating room 4 and hit dish 7, be installed in the motor high-speed driving system 8 on the driving shaft 6.Revolve and hit that dish 7 peripheries are uniform to impact 9 more than 120.4 tops, operating room have sealable discharging opening 10.Sealed bearing 11 is installed between driving shaft 6 and the operating room 4.
Embodiment 1 can prepare powder body, the liquid of various nanometer materials.In the material of preparation<the reaching more than 95% of 100nm.Its preparation technology is simple, and flow process is short, and cost is also low.Can be widely used in the preparation of various nanometer materials.
Embodiment 2.A kind of preparation method of nano-anti-oxidation active poly peptide medicine.It may further comprise the steps:
(1) active poly peptide medicine is placed storage tank, cover seal cover, simultaneously the discharging opening of operating room is built sealing;
(2) storage tank and operating room were immersed in the liquid nitrogen 8 minutes together with active poly peptide medicine;
(3) just storage tank and operating room's evacuation and maintenance;
(4) open bottom valve, make active poly peptide medicine fall into the operating room, close bottom valve then;
(5) actuating motor revolves the motor high-speed driving to hit dish to impact active poly peptide medicine 1.2 minutes, and revolving the rotating speed that hits dish is 30000 rev/mins;
(6) open the discharging opening of operating room, the active poly peptide medicine after will processing with negative pressure subsequently takes out, and preparation finishes.
Wherein the vacuum of step (3) is 6 * 0.01pa.Revolve during work that to hit the linear velocity that dish impacts an outer end be 480m/s.
Embodiment 2 can prepare the anti-oxidation active poly peptide medicine of nano-scale, wherein<and the reaching more than 95% of 100nm.
This medicine has significant rising effect to antioxidase SOD, GSH-px and the CAT of the hairless mouse skin of ultraviolet (UV) A and UVB damage, obviously reduces the content of lipid peroxidation product MDA.Can increase the fibroblastic quantity of hairless mouse skin, raise the Bcl-2 expression of gene, suppress the expression of NOS, mutant P 53 gene and EGFR and be lowered into P content of material in the fiber endochylema, effect is better than common nano-anti-oxidation active polypeptide.
Embodiment 3.A kind of preparation method of nano-anti-oxidation active poly peptide medicine.It may further comprise the steps:
(1) active poly peptide medicine is placed storage tank, cover seal cover, simultaneously the discharging opening of operating room is built sealing;
(2) storage tank and operating room were immersed in the liquid nitrogen 5 minutes together with active poly peptide medicine;
(3) just storage tank and operating room's evacuation and maintenance;
(4) open bottom valve, make active poly peptide medicine fall into the operating room, close bottom valve then;
(5) actuating motor revolves the motor high-speed driving to hit dish to impact active poly peptide medicine 1.5 minutes, and revolving the rotating speed that hits dish is 32000 rev/mins;
(6) open the discharging opening of operating room, the active poly peptide medicine after will processing with negative pressure subsequently takes out, and preparation finishes.
Wherein the vacuum of step (3) is 5 * 0.01pa.Revolve during work that to hit the linear velocity that dish impacts an outer end be 500m/s.
Embodiment 3 can prepare the anti-oxidation active poly peptide medicine of nano-scale, wherein<and the reaching more than 95% of 100nm.
This medicine can significantly improve fibroblastic activity after the ultraviolet injury, improves SOD in the endochylema, and the activity of GSH-px can reduce the activity of XOD simultaneously.Reduce ROS, MDA content, improve A-SAC and T-AOC.Be lowered into fibrocellular apoptosis rate and mortality rate, be reduced to free [Ca2+] i in the fibrocyte, stablize mitochondrial transmembrane potential.
Embodiment 4.A kind of preparation method of nano-anti-oxidation active poly peptide medicine.It may further comprise the steps:
(1) active poly peptide medicine is placed storage tank, cover seal cover, simultaneously the discharging opening of operating room is built sealing;
(2) storage tank and operating room were immersed in the liquid nitrogen 10 minutes together with active poly peptide medicine;
(3) just storage tank and operating room's evacuation and maintenance;
(4) open bottom valve, make active poly peptide medicine fall into the operating room, close bottom valve then;
(5) actuating motor revolves the motor high-speed driving to hit dish to impact active poly peptide medicine 1 minute, and revolving the rotating speed that hits dish is 28000 rev/mins;
(6) open the discharging opening of operating room, the active poly peptide medicine after will processing with negative pressure subsequently takes out, and preparation finishes.
Wherein the vacuum of step (3) is 7 * 0.01pa.Revolve during work that to hit the linear velocity that dish impacts an outer end be 400m/s.
Embodiment 4 can prepare the anti-oxidation active poly peptide medicine of nano-scale, wherein<and the reaching more than 95% of 100nm.
This medicine can be kept lymphocytic normal ultrastructure due to the 60Co radiation, suppress the appearance of lymphocyte DNA ladder-tape, reduce the Lymphocyte Apoptosis rate, significantly improve the activity of SOD in the cytoplasm, GSH-px, reduce ROS, MDA content, improve A-SAC and T-AOC.Reduce free [Ca2+] i in the lymphocyte; Stablize mitochondrial transmembrane potential; Mouse thymus lymphocyte p53 gene protein and Bax albumen are in the expression of endochylema, the proteic expression of enhance Bcl-2 after the inhibition 60Co radiation; Also can suppress the expression of mouse thymus lymphocyte P21WAF1mRNA and P38mRNA after the 60Co radiation simultaneously.Effect is compared with common nano-anti-oxidation active polypeptide, has significant statistics difference.

Claims (4)

1. nanometer materials preparation facilities, it comprises the storage tank, the operating room that is connected with bottom valve that have seal cover and bottom valve, be installed in pumped vacuum systems in storage tank and the operating room, be installed in that to have in the operating room that revolving of driving shaft hit dish, be installed in rotating speed on the driving shaft be 28000~32000 rev/mins motor driven systems, revolve hit dish periphery uniform impact head more than 120, top, operating room has sealable discharging opening, should seal between driving shaft and the operating room.
2. according to the preparation method of the nano-anti-oxidation active poly peptide medicine of the described nanometer materials preparation facilities of claim 1, it is characterized in that may further comprise the steps:
(1) active poly peptide medicine is placed storage tank, cover seal cover, simultaneously the discharging opening of operating room is built sealing;
(2) storage tank and operating room were immersed in the liquid nitrogen 5~10 minutes together with active poly peptide medicine;
(3) just storage tank and operating room's evacuation and maintenance;
(4) open bottom valve, make active poly peptide medicine fall into the operating room, close bottom valve then;
(5) actuating motor revolves the motor high-speed driving to hit dish to impact active poly peptide medicine 1~1.5 minute, and revolving the rotating speed that hits dish is 28000~32000 rev/mins;
(6) open the discharging opening of operating room, the active poly peptide medicine after will processing with negative pressure subsequently takes out, and preparation finishes.
3. according to the preparation method of the described nano-anti-oxidation active poly peptide medicine of claim 2, it is characterized in that the vacuum of said step (3) is (5~7) * 0.01pa.
4. according to the preparation method of the described nano-anti-oxidation active poly peptide medicine of claim 3, it is characterized in that saidly revolving that to hit the linear velocity that dish impacts an outer end be 400~500m/s.
CN 200510045515 2005-12-06 2005-12-06 Nano-grade material preparation apparatus, and method for preparing nano-anti-oxidation active poly peptide medicine Expired - Fee Related CN1785153B (en)

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CN1785153B true CN1785153B (en) 2010-04-21

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6669961B2 (en) * 2000-08-15 2003-12-30 Board Of Trustees Of University Of Illinois Microparticles
US20040091542A1 (en) * 2001-01-18 2004-05-13 Wiwik Watanabe Method for the preparation of nanoparticles
CN1565695A (en) * 2003-06-18 2005-01-19 杨佳荣 Method for producing nanometer particles of plant, traditional Chinese medicine, chemical prescriptions, biological prescriptions
CN1582966A (en) * 2003-08-22 2005-02-23 中科纳米技术工程中心有限公司 Preparation of nano pearls powder

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6669961B2 (en) * 2000-08-15 2003-12-30 Board Of Trustees Of University Of Illinois Microparticles
US20040091542A1 (en) * 2001-01-18 2004-05-13 Wiwik Watanabe Method for the preparation of nanoparticles
CN1565695A (en) * 2003-06-18 2005-01-19 杨佳荣 Method for producing nanometer particles of plant, traditional Chinese medicine, chemical prescriptions, biological prescriptions
CN1582966A (en) * 2003-08-22 2005-02-23 中科纳米技术工程中心有限公司 Preparation of nano pearls powder

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