CN1583166A - Chinese forest frog bactericidal peptide spray agent and its preparation - Google Patents
Chinese forest frog bactericidal peptide spray agent and its preparation Download PDFInfo
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- CN1583166A CN1583166A CN 200410010871 CN200410010871A CN1583166A CN 1583166 A CN1583166 A CN 1583166A CN 200410010871 CN200410010871 CN 200410010871 CN 200410010871 A CN200410010871 A CN 200410010871A CN 1583166 A CN1583166 A CN 1583166A
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- wood frog
- antibiotic peptides
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- frog antibiotic
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Abstract
A spray of tree frog's antibacterial peptide for treating burn, scald, and skin infection is prepared from antibacterial peptide of tree frog, nipagin ethylester, PEG 400, sodium chloride and water through preparing auxiliary solution, adding the antibacterial peptide of tree frog and water, stirring and loading in sprayer.
Description
Technical field
The invention belongs to a kind of antibiotic analgesic and preparation field thereof, particularly a kind of Wood frog antibiotic peptides medicine and the method for making the Wood frog antibiotic peptides spray.
Background technology
(Antimicrobial peptides AMPs) is an important component part of the inner system of defense of organism to antibacterial peptide.Antibacterial peptide has resisting gram-positive bacteria, gram negative bacteria, fungus, bag by the ability of virus, protista and fastbacteria, has been widely used in food fresh keeping and field of medicaments at present.
Amphibian animal living environment complexity is various, and their skin is being kept their existence, opened up and adapt in the wide habitat and play an important role.In order to adapt to living environment, active substance of a great variety, that function is complicated has distributed in the skin of amphibian animal, be a huge active biomolecule resources bank and drug resource storehouse, Jacob L. in 1994 points out that in article the antibacterial components that some batrachia skin produces can kill various tumor cells, its ED
50Be LD
501/10th, safety in utilization is very high.
Skin infection and empyrosis infect and are manyly caused by pseudomonas aeruginosa (bacillus pyocyaneus), mainly at present adopt several antibiotic to unite to make to be used for suppressing pseudomonas aeruginosa.The antibacterial peptide that extracts from Rana sylvatica Le conte skin has the good sterilization bacteriostasis to pseudomonas aeruginosa, and other gram positive bacterias, gram negative bacteria and fungus and clinical multi-drug resistant bacteria are also had certain bactericidal effect.Wood frog antibiotic peptides can also easing pain and diminishing inflammation except that the bactericidal effect.
With the close background technology of the present invention is that publication number is the Chinese patent of CN1216768A, this patent disclosure the preparation method of Wood frog antibiotic peptides.This patent is not made into medicine with Wood frog antibiotic peptides as yet.
Summary of the invention
The technical problem to be solved in the present invention is, Wood frog antibiotic peptides is made the spray medicine, and provide the manufacture method of this medicine, reach the no incompatibility of prescription, convenient drug administration does not need the special posture administration, directly act on disease sites, avoided the first pass effect and the medicine of liver that gastrointestinal is stimulated simultaneously, processing technology is easy, the purpose that the quality of the pharmaceutical preparations is stable.
Wood frog antibiotic peptides spray of the present invention, its raw material, adjuvant and consumption by mass percentage are as follows:
Wood frog antibiotic peptides is 5～40%;
Hydroxyl esters antiseptic is 0.2～3%;
PEG400 (PEG
400) or propylene glycol be 1～5%;
Sodium chloride is 0.45～0.75%;
Surplus is a water.
In the adjuvant, said water can be distilled water or water for injection; Said hydroxyl esters antiseptic is preferably selected ethyl hydroxybenzoate for use; PEG400 or propylene glycol can promote the infiltration of medicine at skin surface.
Wood frog antibiotic peptides spray of the present invention has following feature:
(1) aesthetic appearance: weak yellow liquid; (2) pH value: 3.5～5; (3) stability: at acid condition, 121 ℃ of temperature, pressure 1.01 * 10
5Following 20 minutes of Pa is non-inactivation still; (4) bacteriostatic activity: adopt cup-plate method to carry out antibacterial activity and measure, clinical multidrug resistant pseudomonas aeruginosa and staphylococcus aureus are had inhibitory action.
Wood frog antibiotic peptides spray of the present invention promotes wound healing by bacteria growing inhibiting breeding, control skin or wound infection antiinflammatory, analgesia.Non-infected wound can be sprayed directly on to the affected part with the present invention, and infected wound or burn need to handle the back medication, and there is protective effect in the affected part.
Wood frog antibiotic peptides spray preparing process of the present invention is, takes by weighing raw material, adjuvant by usage ratio, prepares as follows again:
Get hydroxyl esters antiseptic and be dissolved in the suitable quantity of water, be heated to 40～50 ℃ and make it whole dissolvings; Add PEG400 or propylene glycol, sodium chloride, stirring makes molten entirely, and high temperature sterilize is made the adjuvant solution for standby;
Wood frog antibiotic peptides is added in the adjuvant solution, add adding water to full dose, mixing is sub-packed in quantitative aerosol apparatus.
Quantitatively aerosol apparatus can be 5mL or 10mL or 20mL.
Wood frog antibiotic peptides spray of the present invention is mainly used in all kinds of dermatosis and the empyrosis that are caused by pseudomonas aeruginosa and infects, and therapeutical effect is preferably arranged, to by other germ infection certain curative effect being arranged also.
Beneficial effect of the present invention is that formulation and technology is easy, and the quality of the pharmaceutical preparations is stable, the no incompatibility of writing out a prescription, and convenient drug administration does not need the special posture administration, directly acts on disease sites, has avoided the first pass effect of liver and medicine that gastrointestinal is stimulated simultaneously; Splendid with the skin adherence effect, strong in skin surface absorption and permeability, be beneficial to the performance of drug effect, not pollution clothes.The present invention compare with antibiotics have toxic and side effects little, be difficult for diseaseful drug resistance and advantage such as easy to use.This preparation adopts the spray bottle liquid drug that proportioning device is arranged, and is easy to use at clinical expansion.The present invention can be widely used in clinical burn and scald, skin infection and daily household sterilization etc.
The specific embodiment
The present invention is a raw material with the Wood frog antibiotic peptides of molecular weight 5000～20000, and concrete implementation step is as follows, but and does not mean that limitation of the scope of the invention.
Embodiment 1 Wood frog antibiotic peptides consumption is to the influence of bacteriostatic activity
Select ethyl hydroxybenzoate 2.0 grams, PEG400 (PEG for use
400) 10 grams, sodium chloride 2.0 grams are dissolved in ethyl hydroxybenzoate in the water for injection earlier, are heated to about 45 ℃, add PEG
400, sodium chloride, stir and to make moltenly entirely, it is standby to make four parts of adjuvant solution five equilibriums behind the high temperature sterilize; Respectively Wood frog antibiotic peptides 5 grams, 15 grams, 30 grams, 40 grams are joined in four parts of adjuvant solution, add water for injection, be mixed with Wood frog antibiotic peptides and account for about 5%, 15%, 30%, 40% Wood frog antibiotic peptides spray by quality.
Adopt cup-plate method to carry out the pseudomonas aeruginosa antibacterial activity and measure, the result shows: along with the increase of Wood frog antibiotic peptides consumption, antibacterial activity is also strengthening; It is slow that but consumption surpasses the enhancing of 35% antibacterial activity.
The raw material of Wood frog antibiotic peptides spray, the reasonable consumption of adjuvant are as follows:
(1) Wood frog antibiotic peptides is 15～25%;
(2) hydroxyl esters antiseptic is 0.5～2%;
(3) PEG400 or propylene glycol are 1.5～3.5%;
(4) sodium chloride is 0.55～0.65%;
(5) surplus is a water for injection.
Embodiment 2 best proportionings, inhibition zone test, stability test
1, the raw material of Wood frog antibiotic peptides spray, adjuvant optimum amount are as follows:
(1) Wood frog antibiotic peptides is 18%;
(2) ethyl hydroxybenzoate is 1%;
(3) PEG400 is 2.5%;
(4) sodium chloride is 0.6%.
(5) surplus is a water for injection.
2, inhibition zone test
Experiment is to use the Wood frog antibiotic peptides spray of embodiment 2 preparations below
(1) strain
Pseudomonas aeruginosa Pseudomonas aeruginosa ATCC27853
Colon bacillus Escherichia coli ATCC25922
Staphylococcus aureus Staphylocouus aureus ATCC25923
The pseudomonas aeruginosa of clinical multi-drug resistant (main drug resistance title piperacillin, ofloxacin, cefotaxime, sulfamethoxazole, third generation cephalosporin, piperazine ketone)
The staphylococcus aureus of clinical multi-drug resistant (main drug resistance title penicillin, erythromycin, ofloxacin, methicillin)
(2) method
Above-mentioned each spawn culture to exponential phase, is diluted 10 with fluid medium
5～10
6CFU/ml dips in aseptic cotton and to get dilution bacterium liquid and evenly be applied on the solid medium in the aseptic plate.(10 * 7.8 * 6mm) place plate with aseptic Oxford cup, put four Oxford cups in each plate, the negative contrast of Oxford, center cup, put the Wood frog antibiotic peptides spray for three on every side, put in each Oxford cup that to add amount of medicament be 100 μ l, cover the potlery tile lid after, put 37 ℃ and cultivated 16～24 hours, with slide gauge amount antibacterial circle diameter, each experiment repeats 3～5 times.
(3) result
Antibacterial circle diameter (mm)
Bacterium name Wood frog antibiotic peptides spray (100 μ l) negative control (100 μ g)
ATCC27853 15～17.2 -
ATCC25923 14～15.8 -
ATCC25922 14.6～15.8 -
Clinical multi-drug resistant 14.2～16-
Pseudomonas aeruginosa
Clinical multi-drug resistant 13～15-
Staphylococcus aureus
Annotate: the inhibition zone standard---greater than 8mm bacteriostasis is arranged;
There is not bacteriostasis less than 8mm.
3, stability test
The present invention was placed 3 months, 2 months, 1 month under room temperature, 37 ℃, 50 ℃ respectively successively, do bacteriostatic test with said method weekly, strain is the pseudomonas aeruginosa of clinical multi-drug resistant.
Antibacterial circle diameter (mm)
The time 37 ℃ 50 ℃ of room temperatures
1～4 week 14.4～16 14.5～15.8 14.2～15.1
5～8 weeks 14.8～15.6 14.2～15.9---
9～12 weeks 14.2～15.3------
Claims (5)
1, a kind of Wood frog antibiotic peptides spray is characterized in that, be made up of the consumption that raw material and adjuvant reach by mass percentage: Wood frog antibiotic peptides is 5%～40%; Hydroxyl esters antiseptic is 0.2%～3%; PEG400 or propylene glycol are 1%～5%; Sodium chloride is 0.45%～0.75%; Surplus is a water.
According to the described Wood frog antibiotic peptides spray of claim 1, it is characterized in that 2, said raw material and adjuvant reach and be by mass percentage: Wood frog antibiotic peptides is 15～20%; Hydroxyl esters antiseptic is 1～4%; PEG400 or propylene glycol are 1.0～2.0%; Sodium chloride is 0.2～0.8%; Surplus is a water.
According to claim 1 or 2 described Wood frog antibiotic peptides sprays, it is characterized in that 3, said hydroxyl esters antiseptic is an ethyl hydroxybenzoate; Said water is water for injection.
4, a kind of Wood frog antibiotic peptides spray preparing process of claim 1, it is characterized in that, take by weighing raw material, adjuvant by usage ratio, prepare as follows again: get hydroxyl esters antiseptic and be dissolved in an amount of water for injection, be heated to 40～50 ℃ and make it whole dissolvings; Add PEG400 or propylene glycol, sodium chloride, stirring makes molten entirely, and high temperature sterilize is made the adjuvant solution for standby; Wood frog antibiotic peptides is added in the adjuvant solution, add adding water to full dose, mixing is sub-packed in quantitative aerosol apparatus.
5, a kind of application of Wood frog antibiotic peptides spray of claim 1 is characterized in that, the dermatosis and the empyrosis that are used for being caused by pseudomonas aeruginosa infect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410010871 CN1583166A (en) | 2004-05-25 | 2004-05-25 | Chinese forest frog bactericidal peptide spray agent and its preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410010871 CN1583166A (en) | 2004-05-25 | 2004-05-25 | Chinese forest frog bactericidal peptide spray agent and its preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1583166A true CN1583166A (en) | 2005-02-23 |
Family
ID=34600283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410010871 Pending CN1583166A (en) | 2004-05-25 | 2004-05-25 | Chinese forest frog bactericidal peptide spray agent and its preparation |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899600B (en) * | 2005-07-22 | 2010-09-08 | 上海高科联合生物技术研发有限公司 | Antibiotic peptide spray film forming agent and its preparing method |
| CN101491245B (en) * | 2009-02-25 | 2012-06-27 | 吉林大学 | Disinfection preparation containing forest frog antibacterial peptide and preparation method and use thereof |
| CN106377758A (en) * | 2016-08-30 | 2017-02-08 | 林州中农颖泰生物肽有限公司 | Cecropin antibacterial peptide spraying agent and preparation method thereof |
| CN107029210A (en) * | 2017-04-22 | 2017-08-11 | 通化康元生物科技有限公司 | Wood frog's fallopian tube antibacterial peptide oral spray |
| CN108324586A (en) * | 2018-04-08 | 2018-07-27 | 北京皓尔生物科技有限公司 | A kind of antibacterial anti-inflammatory composition and its application |
| CN109091664A (en) * | 2018-09-11 | 2018-12-28 | 林万君 | Wood frog antibiotic peptides gynecological gel |
-
2004
- 2004-05-25 CN CN 200410010871 patent/CN1583166A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899600B (en) * | 2005-07-22 | 2010-09-08 | 上海高科联合生物技术研发有限公司 | Antibiotic peptide spray film forming agent and its preparing method |
| CN101491245B (en) * | 2009-02-25 | 2012-06-27 | 吉林大学 | Disinfection preparation containing forest frog antibacterial peptide and preparation method and use thereof |
| CN106377758A (en) * | 2016-08-30 | 2017-02-08 | 林州中农颖泰生物肽有限公司 | Cecropin antibacterial peptide spraying agent and preparation method thereof |
| CN107029210A (en) * | 2017-04-22 | 2017-08-11 | 通化康元生物科技有限公司 | Wood frog's fallopian tube antibacterial peptide oral spray |
| CN108324586A (en) * | 2018-04-08 | 2018-07-27 | 北京皓尔生物科技有限公司 | A kind of antibacterial anti-inflammatory composition and its application |
| CN109091664A (en) * | 2018-09-11 | 2018-12-28 | 林万君 | Wood frog antibiotic peptides gynecological gel |
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