CN1203754C - Breeding technology of test-tube konjac - Google Patents
Breeding technology of test-tube konjac Download PDFInfo
- Publication number
- CN1203754C CN1203754C CN 03135572 CN03135572A CN1203754C CN 1203754 C CN1203754 C CN 1203754C CN 03135572 CN03135572 CN 03135572 CN 03135572 A CN03135572 A CN 03135572A CN 1203754 C CN1203754 C CN 1203754C
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- Prior art keywords
- bottom set
- days
- culture
- temperature
- taro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 229920002752 Konjac Polymers 0.000 title claims abstract description 21
- 238000005516 engineering process Methods 0.000 title claims abstract description 12
- 238000009395 breeding Methods 0.000 title claims abstract description 7
- 230000001488 breeding effect Effects 0.000 title claims abstract description 7
- 235000010485 konjac Nutrition 0.000 title abstract 3
- 235000001206 Amorphophallus rivieri Nutrition 0.000 title abstract 2
- 239000000252 konjac Substances 0.000 title abstract 2
- 241001312219 Amorphophallus konjac Species 0.000 title 1
- 244000205754 Colocasia esculenta Species 0.000 claims abstract description 19
- 235000006481 Colocasia esculenta Nutrition 0.000 claims abstract description 16
- 235000016709 nutrition Nutrition 0.000 claims abstract description 8
- 230000035764 nutrition Effects 0.000 claims abstract description 8
- 238000005286 illumination Methods 0.000 claims abstract description 5
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims abstract description 4
- 239000005985 Paclobutrazol Substances 0.000 claims abstract description 4
- 238000011010 flushing procedure Methods 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000007943 implant Substances 0.000 claims description 2
- 230000013011 mating Effects 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 2
- 244000247812 Amorphophallus rivieri Species 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000012883 rooting culture medium Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 3
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 description 2
- 229920002581 Glucomannan Polymers 0.000 description 2
- 229940046240 glucomannan Drugs 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 241000234435 Lilium Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a breeding technology of test tube konjac, belonging to the technical field of agricultural biology. The present invention consists of a conventional procedure in which: when the tissue culture seedling of the konjak is subjected to rooting culture, 1-6ppm of paclobutrazol is added into a rooting culture medium MS + NAA5-1mg/L, the mixture is cultured for 20-25 days in an environment with the temperature of more than 20 ℃ and the illumination of 1000LX, and the tuber expands to form a small bulb while the tissue culture seedling roots. When the bulbule stem grows to 2-4 g, dark culture is carried out, the culture temperature is below 15 ℃, and the tissue culture seedling is poured in a bottle for 10-15 days to form mature bulbule stem. Mature small corms are taken out of a bottle and planted into a seedbed after germination accelerating treatment, and nutrition is applied for one period of 30 days at the temperature of more than 20 ℃ and under the condition of proper shielding, wherein the ratio of the applied nutrition is N: P: K: Ca: Mg is 5: 1: 5: 0.5: 1. The breeding technology can harvest 100-200 g of seed taro and 4-5 small seed taro in the year. Has the advantages of high survival rate and reproduction rate of the seed taro, simple management and the like.
Description
Technical field:
The present invention relates to a kind of raising technology of konjaku test-tube taro, belong to agricultural biological technical field.
Background technology:
Konjaku is the unique plant that can synthesize Glucomannan in a large number, and Glucomannan all has extensive effect on industry, agricultural and medicine, health food, more and more cause people's interest and attention, and cultivated area constantly enlarges.But rate height, good strains of seeds serious degradation have restricted the development of this industry in spite of illness to plant taro.Breed virus-free high quality seedling, plant taro and on various plants such as potato, lily, obtained success by biotechnology, and extensively apply to produce, obtained the economic benefit of good social benefit.Konjaku can have been bred out good seed and be transplanted to the seedbed at present and carried out stock breeding by biotechnology, and effective solution is provided for konjaku kind sexual involution, good species taro are in great shortage.But the cultivation management of konjaku tissue cultivating seedling is a very meticulous job, and technology operation and cultivation condition do not note reducing greatly the survival rate and the knot taro rate of tissue cultivating seedling slightly.
Up to now, do not see the open report of breeding the konjaku bottom set in vitro.
Summary of the invention:
Management is difficult, specification requirement is high overcoming when the konjaku tissue cultivating seedling is transplanted for purpose of the present invention, is difficult for the deficiency of being held by support, and a kind of raising technology of managing the konjaku test-tube taro simple, that survival rate is high is provided.
The raising technology of konjaku test-tube taro of the present invention, by the flushing of konjaku explant, sterilization, inoculation, successive transfer culture, culture of rootage, induce bottom set and ripe bottom set bottle outlet to implant the seedbed, breeding stem tuber program is formed, wherein:
The paclobutrazol that adds 1-6ppm during konjaku tissue cultivating seedling culture of rootage in root media MS+NAA5-1mg/L was cultivated 20-25 days in the environment of temperature more than 20 ℃, illumination 1000LX, and stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set.When bottom set grows to 2-4 when gram, secretly to cultivate, cultivation temperature is below 15 ℃, and tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set.Ripe bottom set bottle outlet is implanted the seedbed after vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is suitably sheltered from heat or light more than 20 ℃, and the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1.This numerous the region between the heart and the diaphragm technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.
The flushing of above-mentioned konjaku explant, sterilization, inoculation, successive transfer culture and corresponding condition of culture are same as the prior art.
The present invention breeds bottom set in vitro by biotechnology, has reduced the program that test-tube seedling transplanting is managed this high request, has advantages such as the survival rate of kind of taro and reproduction rate height, management be simple.
Embodiment:
Earlier with tuberous konjaku explant flushing, and be that specification is got the terminal bud part by 0.5cm * 0.5cm, soaked 15 minutes with 75% alcohol-pickled 2～3 seconds and 0.1% mercuric chloride respectively again, use aseptic water washing immediately, konjaku stem tuber explant after the flushing is put into MS+6BA6-0.1mg/L+NAA6-0mg/L, PH5.8 medium, in temperature is more than 20 ℃, cultivates in the environment of illumination 1000LX and got final product once-seedling forming in 45 days.Put into tissue cultivating seedling same medium again and carry out successive transfer culture this moment, and every 20 days subcultures once, the propagation multiple is 1: 4～1: 6.Temperature in the successive transfer culture process is more than 20 ℃, illumination 1000LX.When seedling grows to 3～4cm when high, carry out the plant division cutting, put into root media MS+NAA5-1mg/L culture of rootage 20-25 days of the paclobutrazol that is added with 1-6ppm, stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set.When bottom set grows to 2-4 when gram, secretly to cultivate, cultivation temperature is below 15 ℃, and tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set.Ripe bottom set bottle outlet is implanted the seedbed after conventional vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is suitably sheltered from heat or light more than 20 ℃, and the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1.This raising technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.
Claims (1)
1, a kind of mating system of konjaku test-tube taro, by the flushing of konjaku explant, sterilization, inoculation, successive transfer culture, culture of rootage, induce bottom set and ripe bottom set bottle outlet to implant the seedbed, breeding stem tuber program is formed, it is characterized in that:
1.1 in root media MS+NAA5-1mg/L, add the paclobutrazol of 1-6ppm during konjaku tissue cultivating seedling culture of rootage, in the environment of temperature more than 20 ℃, illumination 1000LX, cultivated 20-25 days, stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set;
1.2 when bottom set grows to 2-4 when gram, secretly cultivate, cultivation temperature is below 15 ℃, tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set;
1.3 ripe bottom set bottle outlet is implanted the seedbed after vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is sheltered from heat or light more than 20 ℃, the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1; This raising technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03135572 CN1203754C (en) | 2003-08-09 | 2003-08-09 | Breeding technology of test-tube konjac |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03135572 CN1203754C (en) | 2003-08-09 | 2003-08-09 | Breeding technology of test-tube konjac |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1493186A CN1493186A (en) | 2004-05-05 |
| CN1203754C true CN1203754C (en) | 2005-06-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03135572 Expired - Fee Related CN1203754C (en) | 2003-08-09 | 2003-08-09 | Breeding technology of test-tube konjac |
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| CN (1) | CN1203754C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355333C (en) * | 2004-09-17 | 2007-12-19 | 中国科学院昆明植物研究所 | Bulbil konjak sprout breeding method |
| CN100370021C (en) * | 2006-02-20 | 2008-02-20 | 云南师范大学 | Capsular coating method for micro test tube corm of konjack |
| CN100469238C (en) * | 2006-02-20 | 2009-03-18 | 云南师范大学 | Method for producing test-tube carmus for high efficiency commercialized prodn. of devilstrongue |
| CN100574611C (en) * | 2007-12-04 | 2009-12-30 | 云南省农业科学院生物技术与种质资源研究所 | A kind of method for breeding konjak fine breed |
| CN102763597B (en) * | 2012-08-15 | 2013-11-20 | 云南省农业科学院生物技术与种质资源研究所 | Two-generation in one year reproduction method for konjac cross breeding |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
| CN103430822B (en) * | 2013-09-09 | 2015-03-18 | 云南省农业科学院生物技术与种质资源研究所 | Aquaculture seed reproduction method for micro seed tubers of konjac |
| CN105393788B (en) * | 2015-12-17 | 2017-12-12 | 昆明瀚钰百林农业科技有限公司 | A kind of cultural method of bulbil type konjac underground bulb high yield blade face bulb |
| CN113973717A (en) * | 2021-11-29 | 2022-01-28 | 云南省热带作物科学研究所 | Germination accelerating and seedling raising method for bulbil konjak tissue culture micro-corms |
-
2003
- 2003-08-09 CN CN 03135572 patent/CN1203754C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1493186A (en) | 2004-05-05 |
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Owner name: KUNMING INST OF BOTANY CAS Free format text: FORMER OWNER: INST. OF BIOLOGICAL TECHNOLOGY, YUNNAN PROV. ACADEMY OF AGRICULTURE Effective date: 20041231 |
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Effective date of registration: 20041231 Address after: 650223 East Road, Kunming, Yunnan Applicant after: Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences Address before: 650223 East Road, Kunming, Yunnan Applicant before: Biological Technology Research Institute of Yunnan Agricultural Academy |
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