CN100370021C - Capsular coating method for micro test tube corm of konjack - Google Patents
Capsular coating method for micro test tube corm of konjack Download PDFInfo
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- CN100370021C CN100370021C CNB2006100106961A CN200610010696A CN100370021C CN 100370021 C CN100370021 C CN 100370021C CN B2006100106961 A CNB2006100106961 A CN B2006100106961A CN 200610010696 A CN200610010696 A CN 200610010696A CN 100370021 C CN100370021 C CN 100370021C
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Abstract
The present invention relates to a capsular coating method for a micro test tube corm of konjaku. The capsular coated micro test tube corm of konjaku is obtained by harvesting the micro test tube corm of the konjaku, sterilizing the micro test tube corm, preliminarily coating the micro corm, smoothening the coated micro corm, coating the micro corm for the second time, capsulating the micro corm, capsulating the micro corm for the second time, capsulating the micro corm for the third time, checking process, packaging a ventilated polyethylene plastic bag and a storage process. The capsular coated micro test tube corm of konjack prepared by the present invention can be used as the seed source of the scaled cultivation of the konjaku. The method mainly solves the problems that the micro test tube corm of konjaku is dehydrated to die after being taken out from a test tube, the storage period is short, diseases caused by the injury after the konjaku is cultivated, etc.
Description
Technical field
The present invention relates to konjaku test tube microballoon stem technical field, specifically the capsule coating method of konjaku test tube microballoon stem.
Background technology
Vegetative bulb, whip taro, the root stock of adopting as the cultivation provenance more in the large-scale production of konjaku.These vegetative organs very easily pass to the offspring with pathogenic bacteria, cause that offspring's plant strain growth is slow, sickness rate is high.Utilizing plant tissue culture to cultivate test-tube plantlet can carry out the konjaku breeding, produces no seed culture of viruses taro, recover good strains of seeds.But the breeding coefficient of konjaku tissue cultured seedling is low, production cost is high, transplanting survival rate is low, has limited the application of konjaku tissue cultured seedling.The laboratory that China is relevant utilizes the konjaku tissue culture technique in recent years, has carried out the research of konjaku tissue cultured seedling, konjaku test-tube taro, konjaku smile taro.Chinese patent application CN99121051.4, publication number CN1295790 discloses mass production of konjaku tissue cultured seedling and tissue cultured seedling transplantation technique; Chinese patent application CN02155216.9, publication number CN1422526 discloses the propagation method of konjaku test-tube taro; Chinese patent application CN03135572.2, publication number 1493186 discloses konjaku test-tube taro breeding technology.First patent solved the production in enormous quantities problem of tissue cultured seedling, but the transplanting survival rate of tissue cultured seedling is low, limited the popularization of tissue cultured seedling.Two patents in back have solved the production problem of test-tube taro and the miniature taro of test tube, solved the low difficult problem of transplanting survival rate of konjaku group training product to a certain extent, but be vulnerable to damage when having retention period weak point, cultivation in actual applications, thereby cause the infection of pathogenic bacteria, the effect of group training is descended.Many diseases in the konjaku, particularly present restive soft rot as a kind of soil-borne disease, if planting body sustains damage in cultivation, is very easy to take place soft rot.The rarely seen Chinese patent application CN01115052.1 of patent of the stem tuber of present plant, piece root, bulb, protocorm, publication number CN1391814A, ginger tuber coating agent and preparation method thereof is disclosed, this patent has solved the soft rot and the oxyuriasis of ginger kind stem tuber process to a certain extent, but not effect in the sprouting control of nutrition supply and stem tuber.The present invention is with dressing, two stage processing of capsuleization, not only make konjaku test tube microballoon stem have longer retention period, and the interpolation by nutritive substance, plant hormone and agricultural chemicals, make konjaku test tube microballoon stem have good nutrient environment at the sprouting initial stage, the sprouting cycle that can control and certain disease resistance.
Summary of the invention
The purpose of this invention is to provide a kind of konjaku test tube microballoon stem that makes and have longer retention period, avoid simultaneously in cultivation to test tube microballoon stem damage, avoid the superinfection of pathogenic bacteria, make the capsule coating method of the more simple konjaku test tube of the cultivation microballoon stem of konjaku test tube microballoon stem.
Technical scheme of the present invention is: (alginic acid is received to utilize alginic acid, konjac polysaccharide) solution, calcium chloride solution, gac, starch, derosal (white bacterium is clear) is a raw material, by the program of results konjaku test tube microballoon stem → microballoon stem sterilization → microballoon stem dressing → microballoon stem secondary dressing dressing → microballoon stem smooth surface → dressing test tube microballoon stem capsule processing → microballoon stem capsule secondary treatment → three processing → checks of microballoon stem capsuleization just → ventilative polyethylene plastic bag packing → storage the konjaku group is trained test tube microballoon stem (comprising underground bulb and the gas green-ball stem) parcel that forms and is capsule dressing bulb.The capsule coating method of konjaku test tube microballoon stem carries out according to the following steps:
1) in vitro the various source of inductive konjaku test tube microballoon stem (comprising gas green-ball stem, root test tube microballoon stem) cuts off from the inducing plant body, and sterilizes 5-10 minute at the potassium permanganate solution or the 0.01-0.03% Babysafe aqueous solution of 0.5-1.0%;
2) konjaku test tube microballoon stem takes out from potassium permanganate solution or Babysafe, roll in Drug coating with roll mode behind the draining a little, the Drug coating composition is an activated carbon: starch: derosal (or m-tetrachlorophthalodinitrile): 10,000 ten thousand unit agricultural streptomycin: fenaminosulf=3-5: 2-6: 0.02-0.05: 0.001-0.006: 0.01-0.07, rolling time are 1-2 minute;
3) after rolling finishes, with unnecessary powder filter, finish the elementary dressing of test tube microballoon stem with 60 purpose screen clothes;
4) the konjaku test tube microballoon stem of elementary dressing is poured on the new filter screen, nutritive medium is sprayed onto the test tube microballoon stem surface of Cotton seeds with atomizer, nutritive medium consists of: with the liquid of 2 times MS solution preparations, the cytokinin that wherein contains 0.05-0.1mg/L, 0.1-0.5mg/L Plant hormones regulators,gibberellins and the naphthylacetic acid of 0.02-0.1mg/L, the time of spraying, rolling is 1-2 minute, finishes the secondary dressing of konjaku test tube microballoon stem;
5) after the secondary dressing is finished, dry slightly, after the drying dressing test tube microballoon stem poured on the 60 purpose filter screens and roll, make its surface comparatively smooth;
6) dispose, the smooth dressing test tube microballoon stem of handling well is poured on the 60 purpose filter screens, roll in the limit, capsule agent and solidifying agent are sprayed in the limit, carry out the capsule processing of konjaku dressing test tube microballoon stem, the capsule agent is 3-10% alginic acid (perhaps sodium alginate, the konjac polysaccharide) aqueous solution, wherein contains the potassium benzoate of 0.001-0.004%, and solidifying agent is the calcium chloride water of 0.4-0.8%; After the drying, repeat 2-5 time slightly.
7) after the capsule processing, the test tube microballoon stem of capsuleization is dry slightly, and the capsule test tube microballoon stem that drying is good puts into polyethylene plastic bag, and plastics bag places freezer to preserve, and temperature of ice house is 5-8 ℃, and retention period is 90-200 days.
The dressing capsule konjaku test tube microballoon stem that takes out from freezer, the bulb that can be used as in the konjaku cultivation uses, and sprouts in after planting 15-30 days.
Mechanism of the present invention is to utilize the adhesive effect of starch under water-soluble condition; gac and sterilant are bonded in the surface of konjaku test tube microballoon stem; form a protective layer to external world; next utilizes the adhesive effect of starch nutritive substance and plant hormone to be bonded in the outside of protective layer; this layer not only provides nutritive substance for the sprouting of konjaku test tube microballoon stem later on; and can provide and break the konjaku dormancy; promote the plant hormone that bulb is taken root; the 3rd utilizes alginic acid to be contained in film forming characteristics under the calcium particle environment; form the dressing test tube microballoon stem of capsuleization; because the brown alga sorrel of capsuleization has certain permeability; not only can stop the moisture loss of konjaku test tube microballoon stem; and can also guarantee the low physiological metabolism of konjaku test tube microballoon stem, guaranteed konjaku test tube microballoon stem keeping at the physiologically active of storage period.Method of the present invention makes konjaku test tube microballoon stem have longer retention period, avoid simultaneously in cultivation to test tube microballoon stem damage, avoid the superinfection of pathogenic bacteria.Make the cultivation of konjaku test tube microballoon stem more simple, reduce the generation of disease simultaneously.
Embodiment
Embodiment 1:
The konjaku test tube microballoon stem of capsule dressing prepares by the following method:
In vitro inductive konjaku test tube microballoon stem cuts off from the inducing plant body under aseptic condition, and at 0.5% disinfecting solution of potassium permanganate 5-10 minute; Konjaku test tube microballoon stem takes out from potassium permanganate solution, roll in Drug coating with roll mode behind the draining a little, the Drug coating composition is an activated carbon: starch: derosal: 10,000 ten thousand unit agricultural streptomycins: fenaminosulf=3: 2: 0.02: 0.001: 0.01, rolling time was 1-2 minute;
After rolling finishes, with unnecessary powder filter, finish the elementary dressing of test tube microballoon stem with 60 purpose screen clothes; The konjaku test tube microballoon stem of elementary dressing is poured on the new filter screen, nutritive medium is sprayed onto the test tube microballoon stem surface of Cotton seeds with atomizer, nutritive medium consists of: with the liquid of 2 times MS solution preparations, the cytokinin that wherein contains 0.05mg/L, 0.1mg/L Plant hormones regulators,gibberellins and 0.02 when the naa of mg/L, the time of spraying, rolling is 1-2 minute, finishes the secondary dressing of konjaku test tube microballoon stem; After the secondary dressing is finished, dry slightly, after the drying dressing test tube microballoon stem poured on the 60 purpose filter screens and roll, make its surface comparatively smooth; The smooth dressing test tube microballoon stem of handling well is poured on the 60 purpose filter screens, roll in the limit, capsule agent and solidifying agent are sprayed in the limit, carry out the capsule processing of konjaku dressing test tube microballoon stem, the capsule agent is the 3% alginic acid aqueous solution, wherein contain 0.001% potassium benzoate, solidifying agent is 0.4% calcium chloride water; After the drying, repeat capsule processing 2-5 time slightly.The test tube microballoon stem of capsuleization is dry slightly, and the capsule test tube microballoon stem that drying is good puts into polyethylene plastic bag, plastics bag places freezer to preserve, and temperature of ice house is 5-8 ℃, and retention period is 90-200 days.
The dressing capsule konjaku test tube microballoon stem that to preserve in freezer takes out 2500 from freezer, by being seeded in the warmhouse booth of 25 * 35cm, the dressing capsule konjaku test tube microballoon stem of all sowings in after planting 15-30 days is sprouted successively, and the accumulative total germination rate of statistics is 98.5% after 25 days.In sowing, transplant 2500 strains of konjaku test-tube plantlet, add up transplanting survival rate after 25 days, transplanting survival rate is 81.7%.
Embodiment 2:
The capsule dressing of konjaku test tube microballoon stem is undertaken by following:
In vitro inductive konjaku test tube microballoon stem cuts off from the inducing plant body under aseptic condition, and at 1.0% disinfecting solution of potassium permanganate 5-10 minute; Konjaku test tube microballoon stem takes out from potassium permanganate solution, roll in Drug coating with roll mode behind the draining a little, the Drug coating composition is an activated carbon: starch: m-tetrachlorophthalodinitrile: 10,000 ten thousand unit agricultural streptomycins: fenaminosulf=5: 6: 0.05: 0.006: 0.07, rolling time was 1-2 minute;
After rolling finishes, with unnecessary powder filter, finish the elementary dressing of test tube microballoon stem with 60 purpose screen clothes; The konjaku test tube microballoon stem of elementary dressing is poured on the new filter screen, nutritive medium is sprayed onto the test tube microballoon stem surface of Cotton seeds with atomizer, nutritive medium consists of: with the liquid of 2 times MS solution preparations, the cytokinin that wherein contains 0.1mg/L, 0.5mg/L Plant hormones regulators,gibberellins and the naphthylacetic acid of 0.1mg/L, the time of spraying, rolling is 1-2 minute, finishes the secondary dressing of konjaku test tube microballoon stem; After the secondary dressing is finished, dry slightly, after the drying dressing test tube microballoon stem poured on the 60 purpose filter screens and roll, make its surface comparatively smooth; The smooth dressing test tube microballoon stem of handling well is poured on the 60 purpose filter screens, roll in the limit, capsule agent and solidifying agent are sprayed in the limit, carry out the capsule processing of konjaku dressing test tube microballoon stem, the capsule agent is 10% konjac polysaccharide (perhaps sodium alginate) aqueous solution, wherein contain 0.004% potassium benzoate, solidifying agent is 0.8% calcium chloride water; After the drying, repeat capsule processing 2-5 time slightly.The test tube microballoon stem of capsuleization is dry slightly, and the capsule test tube microballoon stem that drying is good puts into polyethylene plastic bag, plastics bag places freezer to preserve, and temperature of ice house is 5-8 ℃, and retention period is 90-200 days.
Claims (1)
1. the capsule coating method of a konjaku test tube microballoon stem is characterized in that carrying out according to the following steps:
1) in vitro the various source of inductive konjaku test tube microballoon stem cuts off from the inducing plant body, and sterilizes 5-10 minute at the potassium permanganate solution or the 0.01-0.03% Babysafe aqueous solution of 0.5-1.0%;
2) konjaku test tube microballoon stem takes out from potassium permanganate solution or Babysafe, roll in Drug coating with roll mode behind the draining a little, the Drug coating composition is an activated carbon: starch: derosal or m-tetrachlorophthalodinitrile: 10,000 ten thousand unit agricultural streptomycin: fenaminosulf=3-5: 2-6: 0.02-0.05: 0.001-0.006: 0.01-0.07, rolling time are 1-2 minute;
3) after rolling finishes, with unnecessary powder filter, finish the elementary dressing of test tube microballoon stem with 60 purpose screen clothes;
4) the konjaku test tube microballoon stem of elementary dressing is poured on the new filter screen, nutritive medium is sprayed onto the test tube microballoon stem surface of elementary Cotton seeds with atomizer, nutritive medium consists of: with the liquid of 2 times MS substratum preparations, the cytokinin that wherein contains 0.05-0.1mg/L, 0.1-0.5mg/L Plant hormones regulators,gibberellins and the naphthylacetic acid of 0.02-0.1mg/L, the time of spraying, rolling is 1-2 minute, finishes the secondary dressing of konjaku test tube microballoon stem;
5) after the secondary dressing is finished, dry slightly, after the drying dressing test tube microballoon stem poured on the 60 purpose filter screens and roll, make its surface comparatively smooth;
6) smooth dressing test tube microballoon stem being poured on the 60 purpose filter screens, rolls in the limit, and capsule agent and solidifying agent are sprayed in the limit, and the capsule agent is 3-10% alginic acid or the sodium alginate or the konjac polysaccharide aqueous solution, wherein contains the potassium benzoate of 0.001-0.004%; Solidifying agent is the calcium chloride water of 0.4-0.8%; After the drying, repeat 2-5 time slightly;
7) after the capsule processing, the test tube microballoon stem of capsuleization is dry slightly, and the capsule test tube microballoon stem that drying is good puts into polyethylene plastic bag, and plastics bag places freezer to preserve, and temperature of ice house is 5-8 ℃, and retention period is 90-200 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100106961A CN100370021C (en) | 2006-02-20 | 2006-02-20 | Capsular coating method for micro test tube corm of konjack |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100106961A CN100370021C (en) | 2006-02-20 | 2006-02-20 | Capsular coating method for micro test tube corm of konjack |
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| CN1810956A CN1810956A (en) | 2006-08-02 |
| CN100370021C true CN100370021C (en) | 2008-02-20 |
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| CNB2006100106961A Expired - Fee Related CN100370021C (en) | 2006-02-20 | 2006-02-20 | Capsular coating method for micro test tube corm of konjack |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL2014879B1 (en) * | 2015-05-28 | 2016-06-14 | Fides Bv | Partially encapsulated plant cuttings. |
| CN105379619A (en) * | 2015-10-24 | 2016-03-09 | 施秉县丽康生态农业科技发展有限公司 | Preparation method for test-tube micro-bulbodium of Pinellia ternana |
| CN106561714B (en) * | 2016-11-04 | 2019-05-24 | 昆明学院 | Konjaku grows directly from seeds seed kind seed coat agent and preparation method thereof |
| CN106613131A (en) * | 2016-11-14 | 2017-05-10 | 昆明学院 | Method for producing seed of amorphophallusbulbifer |
| CN113016387A (en) * | 2021-04-06 | 2021-06-25 | 何为 | Plastic package method for bulbous flower |
| CN113287520B (en) * | 2021-05-26 | 2023-01-10 | 大连工业大学 | Method for quickly obtaining konjak seedlings |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61170385A (en) * | 1985-01-24 | 1986-08-01 | Tokio Sato | Production of raw material of konjak by cell culture |
| CN1391814A (en) * | 2001-06-19 | 2003-01-22 | 刘建华 | Ginger tuber coating agent and its preparing method |
| CN1493186A (en) * | 2003-08-09 | 2004-05-05 | 云南省农业科学院生物技术研究所 | Breeding technology of test-tube konjac |
-
2006
- 2006-02-20 CN CNB2006100106961A patent/CN100370021C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61170385A (en) * | 1985-01-24 | 1986-08-01 | Tokio Sato | Production of raw material of konjak by cell culture |
| CN1391814A (en) * | 2001-06-19 | 2003-01-22 | 刘建华 | Ginger tuber coating agent and its preparing method |
| CN1493186A (en) * | 2003-08-09 | 2004-05-05 | 云南省农业科学院生物技术研究所 | Breeding technology of test-tube konjac |
Non-Patent Citations (1)
| Title |
|---|
| 百合种球包衣防治鳞茎病害的试验研究. 诸葛龙等.江西农业学报，第16卷第1期. 2004 * |
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