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CN112553056B - Culture dish capable of automatically supplying nutrient solution for biological experiments - Google Patents

Culture dish capable of automatically supplying nutrient solution for biological experiments Download PDF

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Publication number
CN112553056B
CN112553056B CN202110030309.5A CN202110030309A CN112553056B CN 112553056 B CN112553056 B CN 112553056B CN 202110030309 A CN202110030309 A CN 202110030309A CN 112553056 B CN112553056 B CN 112553056B
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Prior art keywords
rotating shaft
nutrient solution
culture dish
water pipe
water
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CN202110030309.5A
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CN112553056A (en
Inventor
叶远富
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Shandong Qiangsheng Gene Technology Co ltd
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Shandong Qiangsheng Gene Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/40Manifolds; Distribution pieces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a culture dish, in particular to a culture dish capable of automatically supplying nutrient solution for biological experiments. The invention provides a culture dish capable of automatically supplying nutrient solution for biological experiments, which is simple to operate and reduces experimental errors. A culture dish for biological experiments capable of automatically supplying nutrient solution, comprising: the device comprises a base and a supporting frame, wherein the supporting frame is arranged at the top of the base; the lower part of the base is provided with a moving mechanism; the opening and closing mechanism is arranged on the support frame and is matched with the moving mechanism; the storage mechanism is arranged at the top of the support frame and is connected with the opening and closing mechanism. According to the invention, the moving mechanism is matched with the opening and closing mechanism, and the nutrient solution enters the moving mechanism through the opening and closing mechanism, so that the effect of automatically supplying the nutrient solution to the culture dish is realized.

Description

Culture dish capable of automatically supplying nutrient solution for biological experiments
Technical Field
The invention relates to a culture dish, in particular to a culture dish capable of automatically supplying nutrient solution for biological experiments.
Background
The culture dish is a biological experiment container suitable for inoculation, streaking and bacteria separation, can be used for plant materials, microorganism culture and animal cell adherence culture, and a nutrient solution for biological experiment is also called a culture medium, wherein the culture medium is used for providing needed nutrient components for microorganism growth and mainly provides a carbon source (carbohydrate) and a nitrogen source (protein or amino acid), and is similar to food of microorganisms.
Because the nutrient solution is always consumed, the nutrient solution needs to be timely supplied, otherwise, the process of biological experiments and experimental results are affected, the nutrient solution in the existing culture dish is mainly manually added and supplied by an experiment worker in the culture dish, the culture dish without automatic nutrient solution supply is troublesome to operate, and the nutrient solution is difficult to be timely supplied, so that a certain error is caused to the experimental results.
In order to lighten the workload of experiment workers, a culture dish which is simple to operate and can automatically supply nutrient solution for biological experiments and reduce experiment errors is designed.
Disclosure of Invention
In order to overcome the defects that the operation of the existing culture dish is troublesome and the process of biological experiments and experimental results are influenced, the technical problems of the invention are as follows: provides a culture dish capable of automatically supplying nutrient solution for biological experiments, which is simple to operate and reduces experimental errors.
The technical implementation scheme of the invention is as follows: a culture dish for biological experiments capable of automatically supplying nutrient solution, comprising:
the device comprises a base and a supporting frame, wherein the supporting frame is arranged at the top of the base;
the lower part of the base is provided with a moving mechanism;
the opening and closing mechanism is arranged on the support frame and is matched with the moving mechanism;
the storage mechanism is arranged at the top of the support frame and is connected with the opening and closing mechanism.
Further described, the moving mechanism includes:
the lower part of the base is provided with a sliding plate in a sliding manner;
the compression springs are uniformly wound on the sliding plate, the top ends of the compression springs are connected with the sliding plate, and the bottom ends of the compression springs are connected with the base;
a water storage bucket is arranged at the top of the sliding plate;
the rack is arranged on the sliding plate.
Further described, the opening and closing mechanism includes:
the support frame is provided with a water pipe;
the lower part of the water pipe is provided with a bracket;
the clamping block is arranged on the bracket in a sliding manner;
the bracket is provided with a fixing frame;
the fixed frame is rotatably provided with a first rotating shaft;
the spring is arranged in the fixed frame;
the middle part of the first rotating shaft is provided with a ratchet wheel which is connected with a spring;
the unidirectional gear is arranged in the middle of the first rotating shaft and meshed with the rack;
the first ball valve is arranged on the first rotating shaft;
the first torsion spring is wound on the first rotating shaft;
the rotating rod is rotatably arranged on the bracket;
the ratchet is rotationally arranged on the rotating rod and is matched with the clamping block;
the second torsion spring is wound between the rotating rod and the bracket;
and a third torsion spring is wound between the rotating rod and the ratchet.
Further illustratively, the storage mechanism includes:
a water outlet barrel is arranged at the top of the support frame, and the lower part of the water outlet barrel is connected with a water pipe;
a barrel cover is arranged on the top of the water outlet barrel in a threaded manner;
the sampling valve is rotatably arranged at the bottom of the water outlet barrel.
Further, the device also comprises a rotating mechanism, wherein the rotating mechanism comprises:
the middle part of the support frame is internally provided with a second rotating shaft in a rotating way;
the top of the water pipe is provided with a deflector rod;
the lower part of the water pipe is rotatably provided with a third rotating shaft;
the blades are uniformly arranged on the third rotating shaft;
a bevel gear is arranged at the lower part of the second rotating shaft;
the top of the base is provided with a fixing frame;
the upper part of the fixing frame is rotatably provided with a first bevel gear which is meshed with the bevel gear;
pi Daizu, a belt group is connected between the third rotating shaft and the first bevel gear.
Further, the device also comprises a control mechanism, wherein the control mechanism comprises:
a column jacket is arranged between the upper part of the water pipe and the barrel cover;
the water quantity control valve is connected between the column sleeve and the water pipe in a sliding way.
Further, the device also comprises a detection mechanism, wherein the detection mechanism comprises:
the detection block is arranged on the barrel cover;
the detection rod is arranged between the detection block and the barrel cover in a sliding manner;
and the bottom of the detection rod is provided with a buoy.
Further, the water control valve is a damping material.
The beneficial effects are that: 1. according to the invention, the moving mechanism is matched with the opening and closing mechanism, and the nutrient solution enters the moving mechanism through the opening and closing mechanism, so that the effect of automatically supplying the nutrient solution to the culture dish is realized;
2. by arranging the storage mechanism, a sufficient amount of nutrient solution can be filled, the effect of storing the nutrient solution is realized, and the nutrient solution can be sampled and checked in time by rotating the sampling valve, so that the nutrient solution components are ensured not to deteriorate;
3. the first bevel gear drives the second rotating shaft to rotate through the bevel gear, the second rotating shaft drives the deflector rod to rotate, the effect that the deflector rod uniformly stirs nutrient solution in the water outlet bucket is achieved, and meanwhile, the deflector rod can promote the nutrient solution to flow into the water pipe in an accelerating way;
4. the effect of controlling and adjusting the liquid output of the nutrient solution is achieved by manually sliding the water quantity control valve up and down;
5. the residual quantity of the nutrient solution is visualized through the change of the scales on the detection rod, people can detect the residual storage quantity of the nutrient solution in the water bucket only by observing the scales on the detection rod aligned with the detection block, and the nutrient solution can be conveniently and timely supplied to the water bucket.
Drawings
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a schematic perspective view of a moving mechanism according to the present invention.
Fig. 3 is a schematic perspective view of a first part of the opening and closing mechanism of the present invention.
Fig. 4 is a partially enlarged perspective view of fig. a according to the present invention.
Fig. 5 is a schematic perspective view of a second part of the opening and closing mechanism of the present invention.
Fig. 6 is a schematic perspective view of a storage mechanism according to the present invention.
Fig. 7 is a schematic perspective view of a rotary mechanism according to the present invention.
Fig. 8 is a schematic perspective view of a control mechanism according to the present invention.
Fig. 9 is a schematic perspective view of a detection mechanism according to the present invention.
Meaning of reference numerals in the drawings: 1. the device comprises a base, 2, a support frame, 3, a moving mechanism, 31, a sliding plate, 32, a compression spring, 33, a water storage barrel, 34, a rack, 4, an opening and closing mechanism, 41, a water pipe, 42, a support, 43, a clamping block, 44, a first rotating shaft, 45, a fixed frame, 46, a spring, 47, a ratchet, 48, a one-way gear, 49, a first ball valve, 410, a first torsion spring, 411, a rotating rod, 412, a ratchet, 413, a second torsion spring, 414, a third torsion spring, 5, a storage mechanism, 51, a water outlet barrel, 52, a barrel cover, 53, a sampling valve, 6, a rotating mechanism, 61, a second rotating shaft, 62, a deflector rod, 63, a third rotating shaft, 64, a blade, 65, a bevel gear, 66, a fixed frame, 67, a first bevel gear, 68, a belt group, 7, a control mechanism, 71, a column sleeve, 72, a water quantity control valve, 8, a detection mechanism, 81, a detection rod, 82, a float, 83 and a detection block.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1
The utility model provides a culture dish that can supply with nutrient solution automatically for biological assay, as shown in fig. 1, including base 1, support frame 2, mobile mechanism 3, mechanism 4 and storage mechanism 5 open and shut, base 1 top is equipped with support frame 2, and 1 lower part left side of base is equipped with mobile mechanism 3, and 2 left sides of support frame are equipped with mechanism 4 open and shut, and mechanism 4 and mobile mechanism 3 cooperation open and shut, 2 tops of support frame are equipped with storage mechanism 5, and storage mechanism 5 is connected with mechanism 4 open and shut.
When the culture dish is required to be automatically supplied with nutrient solution in a biological laboratory, the culture dish capable of automatically supplying the nutrient solution can be used, firstly, sufficient nutrient solution is added into the storage mechanism 5, when nutrient solution in the moving mechanism 3 is empty, the opening and closing mechanism 4 is in an opened state, along with the nutrient solution in the storage mechanism 5 flowing into the moving mechanism 3 through the opening and closing mechanism 4, the moving mechanism 3 slowly descends, so that the opening and closing mechanism 4 is driven to perform closing movement, when the moving mechanism 3 is filled with the nutrient solution, the moving mechanism 3 stops moving downwards, the opening and closing mechanism 4 is thoroughly closed until the nutrient solution in the moving mechanism 3 is completely absorbed next time, and the steps are repeated, so that the nutrient solution can be automatically supplied to the culture dish.
Example 2
On the basis of embodiment 1, as shown in fig. 2-6, the moving mechanism 3 comprises a sliding plate 31, a compression spring 32, a water storage barrel 33 and a rack 34, wherein the sliding plate 31 is arranged on the left side of the lower part of the base 1 in a sliding manner, the compression spring 32 is uniformly wound on the sliding plate 31, the top end of the compression spring 32 is connected with the sliding plate 31, the bottom end of the compression spring 32 is connected with the base 1, the water storage barrel 33 is arranged on the top of the sliding plate 31, and the rack 34 is arranged on the sliding plate 31.
The opening and closing mechanism 4 is in an opening state at the beginning, nutrient solution flows into the water storage barrel 33 through the opening and closing mechanism 4, along with the increase of the nutrient solution, the compression spring 32 is compressed under the action of gravity, so that the sliding plate 31, the water storage barrel 33 and the rack 34 move downwards, the opening and closing mechanism 4 is closed, and after the nutrient solution in the water storage barrel 33 is completely absorbed, the rack 34 moves upwards, so that the opening and closing mechanism 4 is opened.
The opening and closing mechanism 4 comprises a water pipe 41, a support 42, a clamping block 43, a first rotating shaft 44, a fixed frame 45, a spring 46, a ratchet 47, a one-way gear 48, a first ball valve 49, a first torsion spring 410, a rotating rod 411, a ratchet 412, a second torsion spring 413 and a third torsion spring 414, wherein the water pipe 41 is arranged on the upper left side of the support 2, the support 42 is arranged on the lower portion of the water pipe 41, the clamping block 43 is arranged on the front side of the support 42 in a sliding mode, the fixed frame 45 is rotatably provided with the first rotating shaft 44, the spring 46 is arranged in the fixed frame 45, the ratchet 47 is arranged in the middle of the first rotating shaft 44, the ratchet 47 is connected with the spring 46, the one-way gear 48 is arranged at the middle of the ratchet 47, the one-way gear 48 is meshed with the rack 34, the first ball valve 49 is arranged at the rear of the first rotating shaft 44, the rotating rod 411 is rotatably arranged on the front left side of the support 42, the ratchet 412 is rotatably arranged at the rear of the rotating rod 411, the ratchet 43 is matched with the clamping block 43, the ratchet 411 is provided with the ratchet 411, the second torsion spring 413 is arranged between the rotating rod and the support 42, the ratchet 414 is wound with the third torsion spring 411, and the third torsion spring is arranged at the rear of the torsion spring 413.
At first, first ball valve 49 opening corresponds with water pipe 41, nutrient solution in the water pipe 41 flows into in the water storage bucket 33 through first ball valve 49, when rack 34 moves down and is contacted with unidirectional gear 48, unidirectional gear 48 rotates and drives first pivot 44 to rotate, spring 46 takes place deformation, thereby drive first ball valve 49 and rotate ninety degrees, first ball valve 49 opening does not correspond with water pipe 41, nutrient solution in water pipe 41 stops flowing into in water pipe 33, first pivot 44 rotates and drives ratchet 47 simultaneously and rotates, ratchet 47 drives ratchet 412 to swing downwards round bull stick 411, third torsion spring 414 constantly twists deformation, after the nutrient solution in water storage bucket 33 absorbs completely, people rotate bull stick 411 rightward, when bull stick 411 contacts with fixture 43, fixture 43 upwards moves, fixture 43 resets and blocks bull stick 411 when bull stick 411 passes through fixture 43, second torsion spring 413 takes place deformation, and then make ratchet 412 separate with ratchet 47, at this moment 46 resets and drives first pivot 44 through ratchet 47 and resets, make first ball valve 49 correspond with water pipe 33, then when rack 412 moves up and drives the ratchet wheel 47 again, wherein the utility model 43 resets and drives the ratchet wheel 43 again and does not make the fixture 43 upwards to slide, wherein, the effect of ratchet wheel 47 makes the first pivot 411 upwards moves, the first rotation is realized, the one-way torque spring 411 is realized.
The storage mechanism 5 comprises a water outlet barrel 51, a barrel cover 52 and a sampling valve 53, wherein the water outlet barrel 51 is arranged at the top of the support frame 2, the left side of the lower part of the water outlet barrel 51 is connected with the water pipe 41, the barrel cover 52 is arranged at the top of the water outlet barrel 51 in a threaded manner, and the sampling valve 53 is arranged at the bottom of the water outlet barrel 51 in a rotary manner.
People unscrew the barrel cover 52, then fill sufficient nutrient solution in the water outlet barrel 51, then screw and close the barrel cover 52 again, realize the effect of storing the nutrient solution, through rotating the sampling valve 53, can in time sample the inspection to the nutrient solution, guarantee that the nutrient solution composition does not go bad.
Example 3
On the basis of embodiment 2, as shown in fig. 7-9, the rotary mechanism 6 is further comprised, the rotary mechanism 6 comprises a second rotating shaft 61, a deflector rod 62, a third rotating shaft 63, a vane 64, a bevel gear 65, a fixing frame 66, a first bevel gear 67 and a belt set 68, the second rotating shaft 61 is rotatably arranged in the middle of the supporting frame 2, the deflector rod 62 is arranged at the top of the water pipe 41, the third rotating shaft 63 is rotatably arranged at the lower part of the water pipe 41, the vane 64 is uniformly arranged at the rear part of the third rotating shaft 63, the bevel gear 65 is arranged at the lower part of the second rotating shaft 61, the fixing frame 66 is arranged at the front side of the top of the base 1, the first bevel gear 67 is rotatably arranged at the upper part of the fixing frame 66 and meshed with the bevel gear 65, and the belt set 68 is connected between the front part of the third rotating shaft 63 and the first bevel gear 67.
The potential energy of the downward flowing of the nutrient solution from the water pipe 41 causes the blades 64 to rotate, thereby driving the third rotating shaft 63 to rotate, the third rotating shaft 63 drives the first bevel gear 67 to rotate through the belt group 68, the first bevel gear 67 drives the second rotating shaft 61 to rotate through the bevel gear 65, the second rotating shaft 61 drives the deflector rod 62 to rotate, the effect that the deflector rod 62 uniformly agitates the nutrient solution in the water outlet barrel 51 is achieved, and meanwhile, the deflector rod 62 can also promote the nutrient solution to accelerate to flow into the water pipe 41.
The water tank also comprises a control mechanism 7, wherein the control mechanism 7 comprises a column sleeve 71 and a water quantity control valve 72, the column sleeve 71 is arranged between the upper part of the water pipe 41 and the tank cover 52, and the water quantity control valve 72 is connected between the column sleeve 71 and the water pipe 41 in a sliding manner.
The water quantity control valve 72 is made of damping materials, and the effect of controlling and regulating the liquid quantity of the nutrient solution is achieved by manually sliding the water quantity control valve 72 up and down.
The detecting device is characterized by further comprising a detecting mechanism 8, wherein the detecting mechanism 8 comprises a detecting rod 81, a float 82 and a detecting block 83, the detecting block 83 is arranged on the right side of the front part of the barrel cover 52, the detecting rod 81 is arranged between the detecting block 83 and the barrel cover 52 in a sliding mode, and the float 82 is arranged at the bottom of the detecting rod 81.
In order to timely detect the storage amount of the nutrient solution in the water barrel 51, the change of the scale on the detection rod 81 enables the residual amount of the nutrient solution to be visualized, when the nutrient solution is reduced, the detection rod 81 floats downwards, people only need to observe the scale on the detection rod 81 aligned with the detection block 83 to detect the residual storage amount of the nutrient solution in the water barrel 51, and the nutrient solution can be conveniently and timely supplied to the water barrel 51.
Finally, it should be noted that: the foregoing description is only illustrative of the preferred embodiments of the present invention, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements or changes may be made without departing from the spirit and principles of the present invention.

Claims (6)

1. A culture dish for biological experiments capable of automatically supplying nutrient solution, comprising:
the device comprises a base (1) and a supporting frame (2), wherein the supporting frame (2) is arranged at the top of the base (1);
a moving mechanism (3), wherein the lower part of the base (1) is provided with the moving mechanism (3);
the opening and closing mechanism (4) is arranged on the support frame (2), and the opening and closing mechanism (4) is matched with the moving mechanism (3);
the storage mechanism (5) is arranged at the top of the support frame (2), and the storage mechanism (5) is connected with the opening and closing mechanism (4);
the moving mechanism (3) comprises:
a sliding plate (31), wherein the sliding plate (31) is arranged at the lower part of the base (1) in a sliding manner;
the compression springs (32) are uniformly wound on the sliding plate (31), the top ends of the compression springs (32) are connected with the sliding plate (31), and the bottom ends of the compression springs (32) are connected with the base (1);
a water storage barrel (33), wherein the top of the sliding plate (31) is provided with the water storage barrel (33);
the rack (34) and the sliding plate (31) are provided with racks (34);
the opening and closing mechanism (4) comprises:
a water pipe (41), wherein the water pipe (41) is arranged on the support frame (2);
a bracket (42), wherein the bracket (42) is arranged at the lower part of the water pipe (41);
the clamping block (43) is arranged on the bracket (42) in a sliding manner, and the clamping block (43) is arranged on the bracket;
a fixed frame (45), wherein the fixed frame (45) is arranged on the bracket (42);
a first rotating shaft (44), wherein the fixed frame (45) is rotatably provided with the first rotating shaft (44);
a spring (46), wherein the spring (46) is arranged in the fixed frame (45);
a ratchet wheel (47), wherein the ratchet wheel (47) is arranged in the middle of the first rotating shaft (44), and the ratchet wheel (47) is connected with a spring (46);
a unidirectional gear (48), wherein the unidirectional gear (48) is arranged in the middle of the first rotating shaft (44), and the unidirectional gear (48) is meshed with the rack (34);
a first ball valve (49), wherein the first ball valve (49) is arranged on the first rotating shaft (44);
a first torsion spring (410), wherein the first torsion spring (410) is wound on the first rotating shaft (44);
the rotating rod (411) is rotatably arranged on the bracket (42);
the ratchet (412), the rotating rod (411) is rotatably provided with the ratchet (412), and the ratchet (412) is matched with the clamping block (43);
a second torsion spring (413), wherein the second torsion spring (413) is wound between the rotating rod (411) and the bracket (42);
and a third torsion spring (414), and the third torsion spring (414) is wound between the rotating rod (411) and the ratchet (412).
2. A culture dish for biological experiments capable of automatically supplying a nutrient solution according to claim 1, characterized in that the storage means (5) comprises:
the top of the support frame (2) is provided with a water outlet barrel (51), and the lower part of the water outlet barrel (51) is connected with a water pipe (41);
a barrel cover (52), wherein the top of the water outlet barrel (51) is provided with the barrel cover (52) in a threaded manner;
the sampling valve (53), the bottom of the water outlet barrel (51) is rotatably provided with the sampling valve (53).
3. A culture dish for biological experiments capable of automatically supplying a nutrient solution according to claim 2, further comprising a rotation mechanism (6), wherein the rotation mechanism (6) comprises:
the second rotating shaft (61) is arranged in the middle part of the support frame (2) in a rotating way, and the second rotating shaft (61) is arranged in the middle part of the support frame;
a deflector rod (62), wherein the deflector rod (62) is arranged at the top of the water pipe (41);
a third rotating shaft (63), wherein the lower part of the water pipe (41) is rotatably provided with the third rotating shaft (63);
the blades (64) are uniformly arranged on the third rotating shaft (63);
a bevel gear (65), wherein the bevel gear (65) is arranged at the lower part of the second rotating shaft (61);
the fixing frame (66) is arranged at the top of the base (1);
a first bevel gear (67), wherein the upper part of the fixing frame (66) is rotatably provided with the first bevel gear (67), and the first bevel gear (67) is meshed with the bevel gear (65);
pi Daizu (68), a belt set (68) is connected between the third rotating shaft (63) and the first bevel gear (67).
4. A culture dish for biological experiments capable of automatically supplying a nutrient solution according to claim 3, further comprising a control mechanism (7), wherein the control mechanism (7) comprises:
a column jacket (71) is arranged between the upper part of the water pipe (41) and the barrel cover (52);
the water quantity control valve (72) is connected between the column sleeve (71) and the water pipe (41) in a sliding way, and the water quantity control valve (72) is connected between the column sleeve and the water pipe (41).
5. A culture dish for biological experiments capable of automatically supplying a nutrient solution according to claim 4, further comprising a detection mechanism (8), wherein the detection mechanism (8) comprises:
the detection block (83), the barrel cover (52) is provided with the detection block (83);
a detection rod (81), wherein the detection rod (81) is arranged between the detection block (83) and the barrel cover (52) in a sliding manner;
and a buoy (82), wherein the bottom of the detection rod (81) is provided with the buoy (82).
6. A culture dish for biological experiments capable of automatically supplying a nutrient solution according to claim 4, wherein the water quantity control valve (72) is a damping material.
CN202110030309.5A 2021-01-11 2021-01-11 Culture dish capable of automatically supplying nutrient solution for biological experiments Active CN112553056B (en)

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CN115772471A (en) * 2023-02-13 2023-03-10 山东第一医科大学附属省立医院(山东省立医院) Organ chip culture apparatus capable of adjusting oxygen concentration

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