CN112088875B - Organ preservation device with adjustable solution oxidation-reduction potential and implementation method thereof - Google Patents
Organ preservation device with adjustable solution oxidation-reduction potential and implementation method thereof Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 15
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- 230000007246 mechanism Effects 0.000 claims abstract description 44
- 238000001514 detection method Methods 0.000 claims abstract description 23
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- QUEDXNHFTDJVIY-UHFFFAOYSA-N γ-tocopherol Chemical class OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1 QUEDXNHFTDJVIY-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a solution oxidation-reduction potential adjustable organ preservation device which is characterized by comprising a control box (22), a liquid storage box (1) and a PID controller (23). The invention also discloses a realization method of the organ preservation device with adjustable solution oxidation-reduction potential, which comprises the steps of establishing various index databases of preservation solutions required by preservation of different organs; after the organ is put into the preservation, extracting various index data of preservation fluid of the organ to be preserved from various index databases of preservation fluid, and sending the data to a PID controller (23) for storage and the like. The invention can effectively eliminate harmful components generated by continuous metabolism in the organ preservation process, and ensure the stability of the high polymer with oxygen carrying function in the preservation solution in the organ preservation chamber; meanwhile, through the detection mechanism, the temperature, the pH value, the PO2 and the ORP of the solution of the preservation solution in each chamber can be monitored in real time.
Description
Technical Field
The invention relates to the field of organ preservation, and particularly provides an organ preservation device with an adjustable solution oxidation-reduction potential and an implementation method thereof.
Background
The oxidation-reduction potential (oxidation reduction potential, ORP) is a quantitative indicator representing the oxidation or reduction capacity of a redox system in chemical science. The international general term ORP is expressed in terms of reduction potential, and specifically refers to a physical quantity of a substance that is difficult to get electrons from an oxidized state to a reduced state, as measured by a standard that the potential of a standard hydrogen electrode is zero. There are also various substances in biological systems that affect ORP, such as reactive oxygen species (reactive oxygen species, ROS) in plasma that contribute to the generation of oxidation potential, while reducing agents in plasma, including vitamin C, tocopherols, etc., contribute to the generation of reduction potential. Thus, ORP in biological systems is a comprehensive indicator of the equilibrium between total oxidant and reductant. Under physiological conditions, the internal environment of the human body including various organs, tissues and blood maintains the ORP within a certain range, and the abrupt increase or decrease of the ORP, or the long-term low level or high level, indicates the occurrence or existence of pathological lesions. Therefore, the maintenance of the physiological stability of the environment in the biological system is of great importance to ensure the normal operation of the organ function.
In recent years, the development of aerobic preservation (such as normal temperature mechanical perfusion, low temperature oxygen carrying perfusion or oxygen-enriched low temperature static preservation) based on the consideration of providing enough energy metabolism for isolated organs can reduce ischemia reperfusion injury caused by sudden increase of oxygen after organ transplantation. However, the in-vitro organ aerobic preservation device used at present has poor control of oxygen concentration in preservation or perfusion process, so that the in-vitro organ preservation or perfusion effect is poor. In the Sichuan province technical project subsidization project study, the subject group finds that the oxygen concentration of the preservation solution can be effectively controlled by comprehensively regulating and intervening the changes of ORP, temperature, pH value and PO2 of the preservation solution.
Disclosure of Invention
The invention aims to overcome the defects, and provides an organ preservation device with adjustable solution oxidation-reduction potential and an implementation method thereof, wherein ORP, temperature, pH value and PO2 information of organ preservation solution are detected and evaluated in real time, comprehensive regulation and control intervention is carried out on changes of ORP, temperature, pH value and PO2 of the preservation solution after evaluation, and the technology realizes high-specificity medical organ transplantation preservation and ensures the survival rate of organ transplantation preservation.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: an organ preservation device with adjustable solution redox potential comprising:
a control box, a liquid storage box and a PID controller (23) arranged in the control box; the liquid storage tank consists of an organ containing chamber, an anode chamber and a cathode chamber; the organ-containing chamber is located between the anode chamber and the cathode chamber; preservation liquid index intervention systems are respectively arranged in the organ containing chamber, the anode chamber and the cathode chamber; the organ containing chamber, the anode chamber and the cathode chamber are respectively provided with a detection mechanism; and the PID controller is respectively connected with the detection mechanism, the preservation liquid index intervention system, the anode chamber and the cathode chamber.
The preservation liquid index intervention system comprises an oxygen flow rate controller, a semiconductor heater, a semiconductor refrigerator, a magnetic stirrer, an oxygen supplementing pipe, a semiconductor heating sheet, a semiconductor refrigerating sheet and a magnetic rotor, wherein the oxygen flow rate controller, the semiconductor heater, the semiconductor refrigerator and the magnetic stirrer are respectively arranged in the control box; the oxygen flow rate controller is connected with the oxygen supplementing pipe, the semiconductor heater is connected with the semiconductor heating sheet, the semiconductor refrigerator is connected with the semiconductor cooling sheet, and the magnetic stirrer is connected with the magnetic rotor; the PID controller is respectively connected with the oxygen flow rate controller, the semiconductor heater, the semiconductor refrigerator and the magnetic stirrer.
Further, the detection mechanism comprises a sensor, and a pH probe, a temperature probe, a PO2 probe and an ORP probe which are respectively connected with the sensor; the PID controller is connected with the sensor.
In order to protect the oxygen supplementing tube, the semiconductor heating sheet and the semiconductor refrigerating sheet and ensure the normal operation of the magnetic rotor, support plates are arranged above the oxygen supplementing tube, the semiconductor heating sheet and the semiconductor refrigerating sheet in the organ containing chamber, the anode chamber and the cathode chamber; the magnetic rotor is arranged on the supporting plate; the supporting plate is a mesh plate; a plurality of air passing holes are formed in the pipe wall of the oxygen supplementing pipe, and each air passing hole is covered with a waterproof breathable film.
Still further, a liquid storage box cover matched with the liquid storage box is arranged on the liquid storage box; the sensor is arranged on the liquid storage tank cover; the anode chamber comprises a positive electrode plate arranged on the inner side of one end wall of the liquid storage tank and an anion exchange membrane vertically arranged in the liquid storage tank;
the cathode chamber comprises a negative electrode plate arranged on the inner side of the other end wall of the liquid storage tank and a cation exchange membrane vertically arranged in the liquid storage tank; the organ containing chamber is positioned between the anion exchange membrane and the cation exchange membrane; the PID controller is respectively connected with the positive electrode plate and the negative electrode plate.
In order to facilitate the placement of the organ and the repair of the organ before the transplantation, a liftable organ storage mechanism is also arranged in the organ containing cavity; the organ containing chamber consists of two semipermeable membranes which are vertically and parallelly arranged in the liquid storage tank; a gap is formed between the two semi-permeable membranes; one of the semi-permeable membranes is close to or jointed with the anion exchange membrane, and the other semi-permeable membrane is close to or jointed with the cation exchange membrane; the organ storage mechanism comprises two suspension mechanisms capable of moving up and down, and a storage table with one end connected with one suspension mechanism and the other end connected with the other suspension mechanism; the two suspension mechanisms capable of moving up and down are respectively arranged on the two semipermeable membranes; the suspension mechanism comprises a support arm, a suspension arm which penetrates the support arm from top to bottom and can slide up and down, and a locking screw rod which is arranged on the support arm and used for fixing the suspension arm; the storage tables are respectively connected with the hanging arms; the support arm is fixed on the semipermeable membrane.
In order to ensure better functionality of the device in the soaking and preserving fluid, a plurality of through holes are arranged on the storage table and are uniformly distributed on the storage table. In order to facilitate the use and ensure the stability of the pressure in the liquid storage tank, sampling ports, preservation liquid input ports and gas pressurizing and depressurizing valves are arranged at the upper ends of the rear walls of the organ containing chamber, the cathode chamber and the anode chamber; the lower ends of the rear walls of the organ containing chamber, the cathode chamber and the anode chamber are respectively provided with a liquid outlet; and the PID controller is connected with the gas pressure increasing and reducing valve.
In order to ensure the practicability of the organ preservation device and simultaneously facilitate the installation of the organ survival supply pipe, an organ survival supply port is also arranged on the rear chamber wall of the organ containing chamber; the number of the organ survival supply ports is four-six, and the organ survival supply ports are positioned below the sampling port and the preservation fluid input port. In order to facilitate the observation of the preservation solution and the organ condition in each cavity of the liquid storage tank, the front cavity wall of the liquid storage tank is a glass plate. The PID controller is internally provided with a data real-time display module and a wireless signal transmission module; and a voltage controller is further arranged between the PID controller and the positive electrode plate and between the PID controller and the negative electrode plate.
A method for realizing an organ preservation device with adjustable solution oxidation-reduction potential comprises the following steps:
step 1: establishing various index databases of preservation solutions required by preservation of different organs;
step 2: after the organ to be preserved is placed in the device, extracting various index data of preserving fluid of the organ to be preserved from various index databases of the preserving fluid, and sending the data to a PID controller for storage;
step 3: the PID controller is used for supplying power to the cathode chamber and the anode chamber, so that the preservation solution of the organ storage chamber is subjected to ion exchange with the preservation solution of the cathode chamber and the anode chamber, and a preservation solution index intervention system is started;
step 4: starting a detection mechanism to collect various index information of the preservation liquid of each chamber, and sending the collected various index information of the preservation liquid of each chamber to a PID controller by the detection mechanism;
step 5: the PID controller judges whether various index information of the received preservation solution of each chamber reaches a standard index; if not, adjusting the index of the preservation solution to interfere with the output power of the system; the power supply of the cathode chamber and the anode chamber is stopped, and the preservation solution of each chamber is subjected to ion guide type exchange;
step 6: and maintaining the output state of the preservation fluid index intervention system, and maintaining all indexes of the preservation fluid in each cavity within the set indexes.
Further, the step of establishing the database of various indices of the preservation fluid required for preservation of different organs in the step 1 is as follows:
1) Setting ORP and temperature values required by a plurality of groups of different preserved organs;
2) Collecting the pH value, PO2 and the initial components and concentration values of the preservation solution in each chamber;
3) Collecting the pH value, PO2 and initial components and concentration values of the preservation solution in each chamber when the preservation solution in each chamber reaches different ORP and temperature set values, and establishing a mathematical relationship model;
4) Setting a plurality of groups of pH values and PO2 values required by different preservation organs;
5) Collecting ORP and temperature values of the initial state of the preservation solution in each chamber, and initial components and concentration values of the preservation solution in each chamber;
6) Collecting ORP and temperature values of the preservation solution in each cavity and initial components and concentration values of the preservation solution in each cavity when the preservation solution in each cavity reaches different pH values and PO2 set values, and establishing a mathematical relationship model;
7) Regulating the ORP, temperature, pH value and PO2 to values required by setting up the mathematical relationship model, and recording the initial components and concentrations of the organ preservation solution in each cavity;
8) Obtaining the ORP, temperature, pH value and PO2 databases of the preservation solution required by the preservation of the final different organs according to the in-vitro preservation mathematical relation model of the organs obtained in the steps 3), 6) and 7).
In the step 4, the starting detection mechanism includes: ORP, temperature, pH and PO2 information of the preservation solution in each chamber.
Compared with the prior art, the invention has the following advantages:
(1) The invention can realize continuous metabolism in the organ preservation process by arranging the liquid storage tank consisting of the organ containing chamber, the anode chamber and the cathode chamber, and anions such as oxygen free radicals and acidic substances generated by the continuous metabolism can remove harmful components in preservation liquid by the ion exchange between the anode chamber and the cathode chamber and the preservation liquid of the organ containing chamber, thereby ensuring the stability of high molecules with oxygen carrying function in the preservation liquid in the organ containing chamber; meanwhile, by arranging detection mechanisms in the anode chamber, the cathode chamber and the organ containing chamber respectively, the temperature, the pH value, the PO2 and the ORP of the solution of the preservation solution in each chamber can be monitored in real time.
(2) According to the invention, the preservation liquid index intervention systems respectively arranged in the anode chamber, the cathode chamber and the organ containing chamber are matched with the PID controller and the detection mechanism, so that the effective control of the temperature, the pH value, the PO2 and the ORP of the organ preservation liquid can be realized, the pH value, the oxygen partial pressure and the stability of the total ORP of the organ preservation liquid are ensured, and the survival rate of organ transplantation is improved.
(3) The magnetic stirrer in the preservation liquid index intervention system is matched with the magnetic rotor, and the magnetic stirrer can drive the magnetic rotor to uniformly stir the preservation liquid in each cavity, so that the ion exchange speed of harmful components of the preservation liquid can be well improved, and meanwhile, the oxygen-containing uniformity of the preservation liquid is improved.
(4) The invention also provides a liftable organ storage mechanism in the organ storage chamber, and the organ storage mechanism can facilitate the placement of the preserved organ and the lifting of the organ to a high position by lifting the hanging arm of the organ storage mechanism after the completion of the organ storage, thereby facilitating the organ trimming before the organ transplantation by doctors.
(5) The invention can be used for detecting various indexes of the preservation solution of the isolated organ in specific use, and can also be used for monitoring experimental indexes in the development process of the organ preservation solution.
Drawings
Fig. 1 is a structural diagram of the present invention.
Fig. 2 is a rear view of the present invention.
Fig. 3 is a front view of the present invention.
Fig. 4 is a schematic structural view of the storage platform of the present invention.
Fig. 5 is a control schematic of the present invention.
FIG. 6 is a schematic view of the oxygen supplying tube according to the present invention.
Fig. 7 is a schematic view of a semiconductor heating sheet according to the present invention.
Fig. 8 is a schematic view of a semiconductor heating sheet according to the present invention.
Fig. 9 is a schematic structural view of the pallet of the present invention.
FIG. 10 is a flow chart of a method for implementing the solution redox potential adjustable organ preservation apparatus of the present invention.
The reference numerals in the drawings are:
1-liquid storage tank, 2-negative electrode plate, 3-magnetic stirrer, 4-magnetic rotor, 5-positive electrode plate, 6-sampling port, 7-preservation liquid input port, 8-anion exchange membrane, 9-support arm, 10-hanging arm, 11-storage table, 12-support plate, 13-organ survival supply port, 14-cation exchange membrane, 15-semi-permeable membrane, 16-locking screw, 17-liquid storage tank cover, 18-gas pressurization and depressurization valve, 19-liquid discharge port, 20-glass plate, 21-through hole, 22-control box, 23-PID controller, 24-oxygen flow rate controller, 25-semiconductor heater, 26-semiconductor refrigerator, 27-oxygen supplementing tube, 28-semiconductor heating sheet, 29-semiconductor refrigerating sheet, 30-sensor, 31-pH probe, 32-temperature probe, 33-PO 2 probe, 34-ORP probe, 35-gas permeation hole, 36-voltage controller, 37-waterproof and permeable membrane.
Description of the embodiments
The present invention will be described in further detail with reference to examples, but embodiments of the present invention are not limited thereto.
Examples
As shown in fig. 1 to 9, the organ preservation device with adjustable solution oxidation-reduction potential of the present invention includes a control box 22, a liquid storage box 1, and a PID controller 23. The liquid storage tank 1 consists of an organ storage chamber, an anode chamber and a cathode chamber, and in order to realize the adjustment of the temperature, the pH value, the PO2 and the ORP of the organ preservation liquid, preservation liquid index intervention systems are respectively arranged in the organ storage chamber, the anode chamber and the cathode chamber. As shown in fig. 1, the preservation fluid index intervention system comprises an oxygen flow rate controller 24, a semiconductor heater 25, a semiconductor refrigerator 26, a magnetic stirrer 3 which are respectively arranged in a control box 22, and oxygen supplementing pipes 27, a semiconductor heating sheet 28, a semiconductor refrigerating sheet 29 and a magnetic rotor 4 which are respectively arranged at the bottoms of an organ containing chamber, an anode chamber and a cathode chamber. The oxygen flow rate controller 24 is connected with the oxygen supplementing pipe 27, the semiconductor heater 25 is connected with the semiconductor heating sheet 28, the semiconductor refrigerator 26 is connected with the semiconductor cooling sheet 29, and the magnetic stirrer 3 is connected with the magnetic rotor 4.
In specific implementation, the control box 22 is provided with a device mounting hole and a fixed table in advance, and the PID controller 23, the oxygen flow rate controller 24, the semiconductor heater 25, the semiconductor refrigerator 26 and the magnetic stirrer 3 are all mounted on the corresponding mounting table of the control box 22 through fixing screws. As shown in fig. 5, the PID controller 23 is provided with a display module and a wireless signal transmission module. I.e. the PID controller 23 is a conventional control device having control output, information storage, signal reception and transmission, and information display functions. The PID controller 23 is connected to the oxygen flow rate controller 24, the semiconductor heater 25, the semiconductor refrigerator 26, and the magnetic stirrer 3, respectively. The display module of the PID controller 23 can display the temperature, pH value, PO2 and ORP of the organ preservation solution, so that medical staff can check various indexes of the organ preservation solution conveniently. Meanwhile, the wireless signal transmission module of the PID controller 23 can transmit the temperature, pH value, PO2, ORP value of the received preservation solution to the corresponding mobile device end through the cloud server, for example: mobile devices such as mobile phones and computers. The PID controller 23 can also receive information sent by the mobile device end through the cloud server, and the PID controller 23 can control the working states of the oxygen flow rate controller 24, the semiconductor heater 25, the semiconductor refrigerator 26 and the magnetic stirrer 3 according to the information sent by the mobile device end so as to realize the adjustment of the temperature, the pH value, the PO2 and the ORP of the organ preservation solution. The semiconductor heater 25 and the semiconductor heating sheet 28 in this embodiment are conventional semiconductor heating apparatus components, the semiconductor refrigerator 26 and the semiconductor cooling sheet 29 are also conventional semiconductor cooling apparatus components, and the oxygen flow rate controller 24 is also an existing conventional oxygen flow rate control valve.
In order to eliminate or reduce harmful components in metabolites produced in organs, the invention provides a liquid storage tank 1 for dialyzing preservation liquid, wherein the liquid storage tank 1 consists of an organ containing chamber, an anode chamber and a cathode chamber, and the organ containing chamber is arranged between the anode chamber and the cathode chamber preferentially in the embodiment. In order to seal the entire tank 1, a tank cover 17 is also provided on the tank 1. As shown in fig. 6, in order to improve the uniformity of oxygen output from the oxygen supplementing tube 27, a plurality of air passing holes 35 are formed in the tube wall of the oxygen supplementing tube 27, and each air passing hole 35 is covered with a waterproof and breathable film 37. When in use, the waterproof and breathable film 37 is fixed on the air passing hole 35 through the adhesive, and the waterproof and breathable film 37 can effectively prevent preservation liquid from flowing into the oxygen supplementing tube 27, thereby improving the exhaust effect of the oxygen supplementing tube 27. The oxygen supplementing tube 27 in this embodiment is an E-shaped tube formed by a plurality of branch tubes, and a plurality of air passing holes 35 are uniformly distributed on each branch tube wall of the oxygen supplementing tube 27. The semiconductor heating sheet 28 and the semiconductor cooling sheet 29 are installed between two adjacent branch pipes of the oxygen supplementing pipe 27. Specifically, the oxygen supply tube 27 is used to supply oxygen to each chamber, and may also be used to supplement or verify the introduction of other gases that may be beneficial to organ survival, such as: and introducing hydrogen, nitrogen, mixed gas or the like. The semiconductor heating sheet 28 is matched with the semiconductor heater 25 and the PID controller 23, and is used for adjusting or heating the temperature of the preservation liquid in each chamber. The semiconductor refrigerating plate 29 is matched with the semiconductor refrigerator 26 and the PID controller 23 and used for cooling the preserving fluid of each chamber.
Meanwhile, detection mechanisms capable of respectively detecting the temperature, the pH value, the PO2 and the ORP of the preservation solution are arranged in the three chambers. The detection means is connected to the PID controller 23, and transmits information of the detected temperature, pH, PO2, ORP of the preservation solution to the PID controller 23. The detection mechanism comprises a sensor 30, and a pH probe 31, a temperature probe 32, a PO2 probe 33 and an ORP probe 34 which are respectively connected with the sensor 30. The PID controller 23 is connected to a sensor 30.
In order to protect the oxygen supplementing tube 27, the semiconductor heating sheet 28 and the semiconductor cooling sheet 29, and also to ensure the normal operation of the magnetic rotor 4, the support plate 12 is provided above the oxygen supplementing tube 27, the semiconductor heating sheet 28 and the semiconductor cooling sheet 29 in the organ accommodating chamber, the anode chamber and the cathode chamber. The magnetic rotor is placed on a pallet 12. In order to enable the oxygen output from the oxygen supplementing tube to be well mixed into the preservation solution, as shown in fig. 9, the supporting plate 12 is preferably set to be a mesh plate in this embodiment.
Further, preservation solutions injected into the organ containing chamber, the anode chamber and the cathode chamber in use need to be determined according to actual needs, wherein the preservation solution in the organ containing chamber can be a solution with good oxygen-carrying high molecular components preferentially. Such as: the UW liquid and the HTK liquid can be self-made organ preservation liquid. In order to improve the antibacterial capability of preventing the organ containing chamber, the anode chamber and the cathode chamber, the invention also coats the inner side walls of the organ containing chamber, the anode chamber and the cathode chamber with antibacterial coatings of the multi-fungus peptide in specific production and use.
In order to facilitate the use of the organ storage tank 1, as shown in fig. 1 and 2, sampling ports 6, preservation fluid input ports 7 and gas pressurizing and depressurizing valves 18 are provided at the upper ends of the rear walls of the organ storage chamber, the cathode chamber and the anode chamber. The sampling port 6 is used for inserting a preservation liquid sampling tube. The preservation solution inlet port 7 is used for installing a preservation solution inlet pipe. The gas pressure increasing and reducing valve 18 is connected to a PID controller 23, and the gas pressure increasing and reducing valve 18 adjusts the oxygen pressure in each chamber under the control of the PID controller 23.
Meanwhile, an organ survival supply port is also arranged on the rear chamber wall of the organ containing chamber, the number of the organ survival supply ports 13 can be four-six, and the organ survival supply ports 13 are positioned below the sampling port 6 and the preservation fluid input port 7. In the present embodiment, the number of organ survival feed ports 13 is preferably set to four, and the number of organ survival feed ports 13 may be increased or decreased as needed. The organ survival feeding pipe inserted into the organ survival feeding port 13 may be determined according to the stored organ needs. In a specific use, the diameters of the sampling port 6, the preservation fluid inlet 7 and the organ survival supply port 13 are set as required, and when the diameters of the sampling port 6, the preservation fluid inlet 7 and the organ survival supply port 13 are set, the tightness between the port walls and the mounting pipe fittings is required to be ensured, so that the stability of the pressure of each chamber is prevented. When in use, the organ survival supply port 13 can facilitate the connection of external pipelines and organs, and can serve the requirements of various organ preservation application scenes, such as the installation of pipeline holding pipelines of normal temperature mechanical perfusion, low temperature oxygen carrying perfusion and the like of isolated organs.
In order to facilitate observation of preservation solutions and states of organs in the organ-containing chamber, the cathode chamber, and the anode chamber, the wall of the front face of the tank 1 is provided as a glass plate 20 in the present embodiment.
In practice, the anode chamber comprises a positive electrode plate 5 and an anion exchange membrane 8, as shown in fig. 1. Specifically, the positive electrode plate 5 is fixed on the inside of one of the end walls of the liquid storage tank 1 by a sealant. The positive electrode plate 5 is connected to a PID controller 23. The anion exchange membrane 8 is vertically arranged in the liquid storage tank 1 and is fixed by a fixed sealing adhesive.
Further, as shown in fig. 1, the cathode chamber includes a negative electrode plate 2 and a cation exchange membrane 14. Specifically, the negative electrode plate 2 is fixed on the inner side of the other end wall of the liquid storage tank 1 by a fixing adhesive, and is connected with the PID controller 23. The cation exchange membrane 14 is vertically fixed in the liquid storage tank 1. The organ containing chamber is positioned between the anion exchange membrane 8 and the cation exchange membrane 14, and the organ containing chamber is respectively and tightly connected with the anion exchange membrane 8 and the cation exchange membrane 14. In order to ensure the controllability of the operating voltages of the positive electrode plate 5 and the negative electrode plate 2, a voltage controller 36 is further provided between the PID controller 23 and the positive electrode plate 5 and the negative electrode plate 2.
Meanwhile, as shown in fig. 1, the organ containing chamber is composed of two semi-permeable membranes 15 vertically arranged in parallel in the liquid storage tank 1. A gap is formed between the two semipermeable membranes 15, one semipermeable membrane 15 being adjacent to the anion exchange membrane 8 and the other semipermeable membrane 15 being adjacent to the cation exchange membrane 14. In practical use, one of the semipermeable membranes 15 may be bonded to the anion exchange membrane 8, and the other semipermeable membrane 15 may be bonded to the cation exchange membrane 14.
In use, the mobile device sets each index value of the storage liquid required by the storage organ, and sends the index value to the PID controller 23 for display. The organ is placed in the organ containing cavity, the PID controller 23 controls the magnetic stirrers 3 of the organ containing cavity, the anode cavity and the cathode cavity to work, and the magnetic field generated by the magnetic stirrers 3 pushes the magnetic rotor 4 placed in the cavity to perform circumferential operation, so that the aim of stirring the preservation solution is fulfilled. The temperature, pH value, PO2 and ORP value of the preservation solution are more uniform. During storage, the organs can produce metabolites, i.e. harmful components in the preservation solution, such as: anions such as oxygen radicals, cations and acidic substances. At this time, the semipermeable membrane 15 is located at two sides of the organ containing chamber, so that the semipermeable membrane 15 allows small molecules and ions to freely diffuse into and out of the organ containing chamber, and prevents the large molecules such as proteins, starch and other colloidal components from freely passing through. At this time, the PID controller 23 supplies electric current to the positive electrode plate 5 and the negative electrode plate 2, and the cation exchange membrane 14 in the cathode chamber allows transfer of cations from the organ preservation solution in the organ-containing chamber into the cathode chamber; at the same time, the anion exchange membrane 8 in the anode chamber allows anions to be transferred from the organ preservation solution in the organ-holding chamber into the anode chamber. The anode chamber and the cathode chamber neutralize the ions of harmful components respectively entering, so that the harmful components are well converted into harmless components, and the preservation solution in the organ containing chamber only has stable macromolecules such as protein, starch and other colloid components.
Meanwhile, the pH probe 31, the temperature probe 32, the PO2 probe 33 and the ORP probe 34 in the organ containing chamber, the anode chamber and the cathode chamber respectively detect the temperature, the pH value, the PO2 and the ORP value of the preservation solution in each chamber, detection information of each chamber is transmitted to the PID controller 23 through the sensor 30 of each chamber, the PID controller 23 displays the detection information, and transmits information data to the mobile equipment through the cloud server, medical staff monitors the preservation solution condition of the organ preservation device in real time through the mobile equipment, and the medical staff sends corresponding instructions to the PID controller according to the received information. The PID controller 23 controls the oxygen flow rate controller 24, the semiconductor heater 25, and the semiconductor refrigerator 26 in accordance with the instructions to bring the organ preservation solution to the set temperature, pH, PO2, and ORP. Thereby realizing the effective control of the oxygen concentration in the preservation solution, effectively improving the preservation effect of the organ and improving the survival rate of organ transplantation.
When the temperatures, pH values, PO2, ORP values of the organ containing chamber, the anode chamber, and the cathode chamber are detected as set values, the PID controller 23 stops the current flow to the positive electrode plate 5 and the negative electrode plate 2, and at the same time, stops the voltage increase to the oxygen production flow rate controller 24, the semiconductor heater 25, and the semiconductor refrigerator 26. At this time, the ions in the cathode chamber and the anode chamber on both sides of the organ-containing chamber can communicate with each other through the semipermeable membrane 15, and return to our conventional ion-guided exchange. Because the harmful substances in the cathode chamber and the anode chamber are mostly converted into harmless components, other indexes such as the pH value of an oxidation-reduction potentiometer in the organ containing chamber and the like can be regulated by the slow mutual communication mode of the three chambers, and the waste of preservation liquid can not be caused, so that the problem that the ORP value of the preservation liquid is unstable in the traditional organ preservation device is effectively solved, the demand of the preservation liquid is effectively reduced, and the organ preservation cost is greatly saved.
Still further, in order to facilitate placement of the organ and repair of the organ prior to implantation, a liftable organ storage mechanism is also provided within the organ storage chamber. As shown in fig. 1, the organ storage mechanism includes a hanging mechanism and a storage stand 11. Specifically, the suspension mechanism can move up and down, and the number is two. The two suspension mechanisms are respectively arranged on the two semipermeable membranes 15 in a mode of fixing the two components through bonding or screw fixation, namely, the suspension mechanisms are arranged in one-to-one correspondence with the semipermeable membranes 15. One end of the storage table 11 is attached to one of the hanging mechanisms by means of adhesion, screw fixation, or the like, and the other end is attached to the other hanging mechanism by means of adhesion, screw fixation, or the like. As shown in fig. 4, in order to ensure that the preservation solution can better protect the organ, a plurality of through holes 21 are provided on the storage table 11, and the plurality of through holes 21 are uniformly distributed on the storage table 11. The pore size of the through hole 21 is set according to the use requirement.
The suspension mechanism is shown in fig. 1, and comprises a support arm 9, a hanging arm 10 and a locking screw 16. Specifically, the support arm 9 may be fixed to the semipermeable membrane 15 by means of adhesion or screw fixation, or in a specific use, the mounting end of the support arm 9 may sequentially pass through the semipermeable membrane 15 and the ion exchange membrane 14 or the anion exchange membrane 8 for better stability of the support arm 9, and then may be fixed by a nut screwed to the support arm 9. The hanging arm 10 penetrates the support arm 9 from top to bottom and can slide up and down along the wall of the penetrating hole. One end of the storage table 11 is mounted at the bottom end of one of the hanging arms 10, and the other end of the storage table 11 is mounted at the bottom end of the other hanging arm 10. The locking screw 16 is arranged on the support arm 9 and used for fixing the hanging arm 10, and the locking screw 16 penetrates through the mounting opening of the hanging arm 10 for fixing the hanging arm 10 and is movably connected with the hanging arm 10. When the device is used, the hanging arm 10 is lifted up, the storage table 11 can be lifted to the chamber opening of the organ containing chamber, and the storage table 11 can be fixed to the chamber opening of the organ containing chamber by locking the locking screw 16. After the organ is placed on the storage table 11 and the organ storing and supplying tube is connected with the organ through the organ storing and supplying port 13, the locking screw 16 is released to move the arm 10 downward, so that the storage table 11 can be placed in the preservation solution.
Examples
As shown in fig. 10, this embodiment is a method for realizing the organ preservation apparatus with adjustable solution oxidation-reduction potential of embodiment 1, comprising the steps of:
step 1: and establishing various index databases of preservation solutions required by preservation of different organs.
Specifically, the steps for establishing various index databases of preservation solutions required by preservation of different organs are as follows:
1) Setting ORP and temperature values required by a plurality of groups of different preserved organs; wherein, according to the environmental temperature differences occurring in different seasons, four groups of ORP and temperature values are set for the preservation solution of each organ.
2) And collecting the pH value, PO2 and the initial components and concentration values of the preservation solution in each chamber in the initial state of the preservation solution in each chamber.
3) When the preservation solution in each cavity reaches different ORP and temperature set values, the pH value, PO2 and the initial components and concentration values of the preservation solution in each cavity are collected, and a mathematical relation model is established.
4) Setting a plurality of groups of pH values and PO2 values required by different preservation organs; wherein, according to the environmental temperature differences occurring in different seasons, four groups of pH values and PO2 values of the preservation solution of each organ are set.
5) ORP and temperature values of the initial state of the preservation solution in each chamber are collected, and initial components and concentration values of the preservation solution in each chamber are collected.
6) When the preservation solution in each cavity reaches different pH values and PO2 set values, the ORP and temperature values of the preservation solution in each cavity and the initial components and concentration values of the preservation solution in each cavity are collected, and a mathematical relation model is established.
7) The ORP, temperature, pH and PO2 are adjusted to values required to meet the settings of the mathematical relationship model described above, and the initial components and concentrations of the organ preservation solution in each chamber are recorded.
8) Obtaining the ORP, temperature, pH value and PO2 databases of the preservation solution required by the preservation of the final different organs according to the in-vitro preservation mathematical relation model of the organs obtained in the steps 3), 6) and 7).
After the previous work is done, the organ preservation work can be carried out in the following steps.
Step 2: after the organ is placed in the preservation apparatus, various index data of the preservation solution of the organ to be preserved is extracted from various index databases of the preservation solution, and sent to the PID controller 23 for storage.
Step 3: the PID controller 23 supplies power to the cathode chamber and the anode chamber, so that the preservation solution of the organ storage chamber and the preservation solution of the cathode chamber and the anode chamber are subjected to ion exchange, and the preservation solution index intervention system is started.
Step 4: the detection mechanism is started to collect various index information of the preservation liquid of each chamber, and the detection mechanism sends the collected various index information of the preservation liquid of each chamber to the PID controller 23.
Specifically, the pH probe 31, the temperature probe 32, the PO2 probe 33, and the ORP probe 34 of the start-up detection means detect the temperature, the pH value, the PO2, and the ORP value of the preservation liquid in each chamber, respectively. The detection information of each chamber is transmitted to the PID controller 23 through the sensor 30 of each chamber.
Step 5: the PID controller (23) judges whether various index information of the preservation solution of each chamber received reaches a standard index; if not, adjusting the index of the preservation solution to interfere with the output power of the system; the power supply to the cathode chamber and the anode chamber is stopped, and the preservation solution in each chamber is subjected to ion-guided exchange.
Step 6: maintaining the output state of the preservation fluid index intervention system, and maintaining all indexes of the preservation fluid in each cavity within the set indexes; i.e. the temperature, pH, PO2, ORP values of the preservation solution in each chamber are kept within the set range, and the organ is in the in vivo survival state.
As described above, the present invention can be advantageously practiced.
Claims (6)
1. An organ preservation device with adjustable solution oxidation-reduction potential is characterized by comprising a control box (22), a liquid storage box (1) and a PID controller (23) arranged in the control box (22); the liquid storage tank (1) consists of an organ containing chamber, an anode chamber and a cathode chamber; the organ-containing chamber is located between the anode chamber and the cathode chamber; preservation liquid index intervention systems are respectively arranged in the organ containing chamber, the anode chamber and the cathode chamber; the organ containing chamber, the anode chamber and the cathode chamber are respectively provided with a detection mechanism; the PID controller (23) is respectively connected with the detection mechanism, the preservation liquid index intervention system, the anode chamber and the cathode chamber;
the preservation liquid index intervention system comprises an oxygen flow rate controller (24), a semiconductor heater (25), a semiconductor refrigerator (26) and a magnetic stirrer (3) which are respectively arranged in a control box (22), and an oxygen supplementing tube (27), a semiconductor heating sheet (28), a semiconductor refrigerating sheet (29) and a magnetic rotor (4) which are respectively arranged at the bottoms of an organ accommodating chamber, an anode chamber and a cathode chamber; the oxygen flow rate controller (24) is connected with the oxygen supplementing tube (27), the semiconductor heater (25) is connected with the semiconductor heating sheet (28), the semiconductor refrigerator (26) is connected with the semiconductor refrigerating sheet (29), and the magnetic stirrer (3) is connected with the magnetic rotor (4); the PID controller (23) is respectively connected with the oxygen flow rate controller (24), the semiconductor heater (25), the semiconductor refrigerator (26) and the magnetic stirrer (3);
the detection mechanism comprises a sensor (30), and a pH probe (31), a temperature probe (32), a PO2 probe (33) and an ORP probe (34) which are respectively connected with the sensor (30); the PID controller (23) is connected with the sensor (30);
the support plates (12) are arranged above the oxygen supplementing tube (27), the semiconductor heating sheet (28) and the semiconductor refrigerating sheet (29) in the organ accommodating chamber, the anode chamber and the cathode chamber; the magnetic rotor is arranged on the supporting plate (12); the supporting plate (12) is a mesh plate; a plurality of air passing holes (35) are formed in the pipe wall of the oxygen supplementing pipe (27), and a waterproof breathable film (37) is covered on each air passing hole (35); the liquid storage tank (1) is provided with a liquid storage tank cover (17) matched with the liquid storage tank; the sensor (30) is arranged on the liquid storage tank cover (17); the anode chamber comprises a positive electrode plate (5) arranged on the inner side of one end wall of the liquid storage tank (1), and an anion exchange membrane (8) vertically arranged in the liquid storage tank (1);
the cathode chamber includes a negative electrode plate (2) provided on the inside of the other end wall of the reservoir (1), and a cation exchange membrane (14) vertically provided in the reservoir (1); the organ containing chamber is positioned between the anion exchange membrane (8) and the cation exchange membrane (14); the PID controller (23) is respectively connected with the positive electrode plate (5) and the negative electrode plate (2);
the organ accommodating chamber is internally provided with a liftable organ accommodating mechanism; the organ containing chamber consists of two semipermeable membranes (15) which are vertically and parallelly arranged in the liquid storage tank (1); a gap is formed between the two semi-permeable membranes (15); one of the semi-permeable membranes (15) is close to the anion exchange membrane (8) or is attached to the anion exchange membrane (8), and the other semi-permeable membrane (15) is close to the cation exchange membrane (14) or is attached to the cation exchange membrane (14); the organ storing mechanism comprises two hanging mechanisms capable of moving up and down, and a storing table (11) with one end connected with one hanging mechanism and the other end connected with the other hanging mechanism; the two suspension mechanisms capable of moving up and down are respectively arranged on the two semipermeable membranes (15); the suspension mechanism comprises a support arm (9), a suspension arm (10) which penetrates the support arm (9) from top to bottom and can slide up and down, and a locking screw (16) which is arranged on the support arm (9) and used for fixing the suspension arm (10); the storage tables (11) are respectively connected with the hanging arms (10); the support arm (9) is fixed on the semipermeable membrane (15).
2. The solution redox potential adjustable organ preservation apparatus according to claim 1, wherein: a plurality of through holes (21) are formed in the storage table (11), and the through holes (21) are uniformly distributed on the storage table (11); the upper ends of the rear walls of the organ containing chamber, the cathode chamber and the anode chamber are respectively provided with a sampling port (6), a preservation solution input port (7) and a gas pressure increasing and reducing valve (18); the lower ends of the rear walls of the organ containing chamber, the cathode chamber and the anode chamber are respectively provided with a liquid outlet (19); the PID controller (23) is connected with the gas pressure increasing and reducing valve (18); an organ survival supply port (13) is also arranged on the rear chamber wall of the organ containing chamber; the number of the organ survival supply ports (13) is four-six, and the organ survival supply ports (13) are positioned below the sampling port (6) and the preservation fluid input port (7).
3. The solution redox potential adjustable organ preservation apparatus according to claim 2, wherein: the front cavity wall of the liquid storage tank (1) is a glass plate (20); a data real-time display module and a wireless signal transmission module are arranged in the PID controller (23); a voltage controller (36) is further arranged between the PID controller (23) and the positive electrode plate (5) and the negative electrode plate (2).
4. The method for realizing the organ preservation apparatus with adjustable solution oxidation-reduction potential according to any one of claims 1 to 3, comprising the steps of:
step 1: establishing various index databases of preservation solutions required by preservation of different organs;
step 2: after the organ to be preserved is placed in the device, extracting various index data of preserving fluid of the organ to be preserved from various index databases of the preserving fluid, and sending the data to a PID controller (23) for storage;
step 3: supplying power to the cathode chamber and the anode chamber through a PID controller (23), performing ion exchange between preservation solution of the organ storage chamber and preservation solution of the cathode chamber and the anode chamber, and starting a preservation solution index intervention system;
step 4: starting a detection mechanism to collect various index information of the preservation liquid of each chamber, and sending the collected various index information of the preservation liquid of each chamber to a PID controller (23);
step 5: the PID controller (23) judges whether various index information of the preservation solution of each chamber received reaches a standard index; if not, adjusting the index of the preservation solution to interfere with the output power of the system; the power supply of the cathode chamber and the anode chamber is stopped, and the preservation solution of each chamber is subjected to ion guide type exchange;
step 6: and maintaining the output state of the preservation fluid index intervention system, and maintaining all indexes of the preservation fluid in each cavity within the set indexes.
5. The method for realizing an organ preservation apparatus with adjustable solution redox potential according to claim 4, wherein the step of establishing various index databases of preservation solutions required for preservation of different organs in the step 1 comprises the steps of:
1) Setting ORP and temperature values required by a plurality of groups of different preserved organs;
2) Collecting the pH value, PO2 and the initial components and concentration values of the preservation solution in each chamber;
3) Collecting the pH value, PO2 and initial components and concentration values of the preservation solution in each chamber when the preservation solution in each chamber reaches different ORP and temperature set values, and establishing a mathematical relationship model;
4) Setting a plurality of groups of pH values and PO2 values required by different preservation organs;
5) Collecting ORP and temperature values of the initial state of the preservation solution in each chamber, and initial components and concentration values of the preservation solution in each chamber;
6) Collecting ORP and temperature values of the preservation solution in each cavity and initial components and concentration values of the preservation solution in each cavity when the preservation solution in each cavity reaches different pH values and PO2 set values, and establishing a mathematical relationship model;
7) Regulating the ORP, temperature, pH value and PO2 to values required by setting up the mathematical relationship model, and recording the initial components and concentrations of the organ preservation solution in each cavity;
8) Obtaining the ORP, temperature, pH value and PO2 databases of the preservation solution required by the preservation of the final different organs according to the in-vitro preservation mathematical relation model of the organs obtained in the steps 3), 6) and 7).
6. The method for realizing an organ preservation apparatus with adjustable solution redox potential according to claim 5, wherein the step 4 of starting the detection mechanism for various index information of preservation solution of each chamber comprises: ORP, temperature, pH and PO2 information of the preservation solution in each chamber.
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