CN104186459A - Sub-normal-temperature or low-temperature isolated kidney storage device - Google Patents
Sub-normal-temperature or low-temperature isolated kidney storage device Download PDFInfo
- Publication number
- CN104186459A CN104186459A CN201410384703.9A CN201410384703A CN104186459A CN 104186459 A CN104186459 A CN 104186459A CN 201410384703 A CN201410384703 A CN 201410384703A CN 104186459 A CN104186459 A CN 104186459A
- Authority
- CN
- China
- Prior art keywords
- organ
- temperature
- storage box
- storage device
- subnormal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a kidney storage device and provides a sub-normal-temperature or low-temperature isolated kidney storage device comprising an organ storage box, a liquid storage chamber, a perfusing pipeline and a controller, wherein the liquid storage chamber is connected with an input opening of the perfusing pipeline through a one-way valve; an output opening of the perfusing pipeline is connected with the organ storage box through a one-way valve; the organ storage box is connected with the input opening of the perfusing pipeline through a one-way valve; the organ storage box is also connected with a urine and perfusate collecting and sampling window; a detecting device connected with the controller is arranged between the perfusing pipeline and the organ storage box; and the organ storage box is provided with a temperature control device. The sub-normal-temperature or low-temperature isolated kidney storage device is used for storing kidneys.
Description
Technical field
The present invention relates to a kind of organ save set, relate in particular to the special perfusion liquid perfused organ of a kind of use to maintain and/or to recover organ viability and activity, be convenient to Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature or the low temperature storage device of storage and/or transportation organ.
Background technology
Organ transplant is the effective means for the treatment of organ failure in whole latter stage, and the donor of high-quality is the successfully basic condition of organ transplant, so organ preservation and vascular anastomosis and immunosupress technology be called three large foundation stones of organ transplant.Donor organ inevitably will experience the process of in vitro ischemic, the bracket for blood grouping of donor and tissue matching, receptor's selection, the confession transportation of transplant organ are, the enforcement of transfer operation etc. all need one period of stand-by period, therefore the object that organ is preserved maintains organ maximum activity at donor between in vitro ischemic stage exactly, makes it after recovering blood flow, can obtain best function.
After kidney is in vitro, oxygen and various metabolism substrate are ended supply, and the energy of storing in cell exhausts rapidly, and then causes the Na on cell membrane
+-K
+aTP enzyme (sodium pump) malfunctioning.There is swollen rising in mitochondria subsequently, lysosomal enzyme discharges, and degradation of cell composition, makes cell structure occur progressive injury, this ischemic damage is reversible within the scope of certain hour, but surpass certain limit, surpassed the critical point of cellular damage recoverability, will cause irreversibility infringement, show as ischemic organ blood vessel endothelium oedema, necrosis, come off, cause the difficulty of pouring into again, finally recover to occur after blood flow " not reflow Phenomenon ", cause primary nonfunctional after the transplanting of organ.The complexity that ischemic injuries relates to each systems such as physiology, biochemistry, immunity changes, but its essence caused pathophysiological change process that is organ energy exhaustion.
Therefore the aim that organ is preserved comprises two aspects: alleviate the energy consumption of organ during in vitro, or during in vitro energy outside supplementary quota.Low temperature static store method in clinical store method, reduces the energy consumption of organ as a kind of prolonged application just by low temperature,, thus the conserving time limit of prolongation organ.10 ℃ of the every reductions of activity with temperature of the most of enzymes of human body just reduce 25～50%, and when temperature is down to 0 ℃ from 37 ℃, the rate of metabolism of organ is suppressed to below 10%.The minimizing of kidney oxygen consumption and the reduction of temperature are exponential relationship, while reducing to 15%, 10 ℃ when oxygen consumption reduces to 40%, 20 ℃ in the time of 30 ℃, are less than 5%.
The speed of cell death but low temperature can only slow down, and can not prevent the death of cell.Low temperature itself also can cause histocyte infringement.When temperature drops to below 20 ℃, cell membrane sex change causes cell dehydration, and in born of the same parents, biochemical reaction rate declines.When penetration activity material density raises, water is got back to cell interior, and cell membrane tension force may lose its barrier function after reaching critical point.The function that after birth can break and cause its adjusting that loses the initiative to adapt to.When temperature drops to below 8 ℃, corresponding change also can occur endochylema becomes gel.At this temperature, molecular diffusion and active transport speed and biochemical reaction rate all greatly reduce.When temperature drops to-3 ℃, if there is no antifreezing agent, the inside and outside water of born of the same parents starts to freeze, and causes membrane structure to break.
Low temperature is when reducing enzymic activity, also suppressed the activity of the adenylic acid on mitochondrial inner membrane (AN) transferase and limited the aggregate velocity of ATP, its result causes ADP in cell, accumulate and be decomposed into AMP by adenylate kinase, further resolve into oxypurine, cause the organ preserved during preservation and the shortage of energy between flush phase again.Under low temperature condition, ischemic also can irritation cell anaerobic metabolism, and in cell, a large amount of lactic acid is piled up, and causes tissue acidosis.Acidosis can cause lysosome stability decreases, lysosomal hydrolase activation, and change mitochondrial character, thus finally cause cell death.The organ of preserving through low temperature, after perfusion again, can produce a large amount of oxygen radicals, by 1. cell membrane lipid bilayer, be oxidized generation MDA, coup injury cell; 2. damaging cells device film, causes that organelle breaks; 3. cause cellular damage, discharge various enzymes and cell factor, cell factor can mediate neutrophil leucocyte assembles and sticks at blood vessel endothelium, causes the approach such as microcirculation disorder to cause transplant organ functional disorder.The minimizing of the polyphenoils such as the hepatocuprein generating at low temperatures in addition,, reduced glutathione has also increased the infringement of oxygen radical.
Therefore under low temperature static is preserved, the caused various histocyte pathology of energy deficiency changes still and is continuing.By the improvement to organ preservative fluid, can suitably alleviate the acidosis of cell, reduce the generation of oxygen radical, but still do not reach clinical needed organ preservation effect.
It is the another kind of store method occurring in recent years that continuous hypothermical perfusion is preserved, by isolated organ being stored in a special irrigator that has pulsed or non-pulse formula, with cold-penetration fluid injection, through organ vascular system, continue circumfusion, to reaching the required basic nutrition of metabolism under supply low temperature, remove the object of relevant refuse.This store method fails really to solve the energy consumption problem of isolated organ equally, although therefore have certain effect aspect the holding time extending organ, but still can not meet current clinical needs.
The U.S. Patent No. 5599659 that application people is Brasile etc. also discloses the preservation solution of preserving tissue, explant and endothelial cell for heat.Brasile has waited the shortcoming of preserving for low temperature, and suggestion adopts hot Techniques of preserving as an alternative solution, and has introduced a kind ofly for preserving the medium of organizing vascular endothelium medium, and at 30 ℃, hot perfused organ is 12 hours.But the flushing of under anaerobic conditions, using this chemicals is not enough to stop or repairing ischemia injury.In addition, utilize crystalloid perfusion liquid and do not fill oxygen, by or not by computer control, at the temperature lower than normal body temperature, realized organ preservation.For example, referring to United States Patent (USP) 5149321,5395314,5584804,5709654 and 5572929 and United States Patent (USP) Shen NO.08/848,601(authorizes the people such as Klatz), these documents are all included in herein as a reference.The infringement that reduces oxygen can also be by adding antioxidant to realize in perfusion liquid.Specifically verified this can be used for reducing the organ damage after warm ischemia time (warm ischemia).Also reported that in addition other perfusion liquid additives can improve the result of machine perfusion.
Along with the progress of organ transplantation technique and the development of kidney replacement therapy, wait for that patient's quantity of transplanting continues to increase, organ shortage is increasingly sharpened.Many advanced ages, hot ischemic lose heavier edge donor organ and are also forced to be widely used in clinical.With China's national situation analysis, the organ that heart death donor is contributed (Donation after Cardiac Death) will become the main source of China's donor organ in recent years.These organs obviously improve the requirement of preserving, although at low temperatures these organs are entered to machine perfusion, have shown obvious improvement, can really meet clinical demand far away.For example, when organ activity is assessed, hypothermia perfusion only can provide indirectly data, can not effectively reflect the activity of organ after replanting.
Summary of the invention
For above-mentioned prior art, the technical problem to be solved in the present invention is to provide a kind ofly pours into one or more organs to maintain and/or to recover organ viability and activity by one or more special perfusion liquid under to a certain degree lower than the equilibrium temperature of body temperature, be convenient to storage with or transportation organ, the Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature or the low temperature storage device that this isolated organ or tissue activity are monitored, recorded simultaneously.According to this device the data obtained and the active monitoring arrangement of histoorgan, determine whether this organ is suitable for transplanting simultaneously, and provide necessary Data support and data evidence for performing the operation.
The present invention is by the following technical solutions to achieve these goals:
A kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature or low temperature storage device, it is characterized in that: comprise organ storage box, fluid storage compartment, priming line, controller, described fluid storage compartment is connected with priming line input port by check valve, and priming line delivery outlet is connected with organ storage box by check valve, organ storage box is connected with priming line input port by check valve, and described organ storage box is also connected with urine urine collecting and sample window; The sniffer of setting and controller between described priming line and organ storage box, described organ storage box is provided with temperature control equipment.
In such scheme, described temperature control equipment is controlled at subnormal temperature 4-32 degree Celsius or closing temperature control device by organ storage box temperature, at organ storage box, arranges that ice cube is controlled at 0-4 degree Celsius by temperature around.(can be when obtaining the concrete state of donor organ and required roughly conserving time limit, select applicable preservation mode)
In such scheme, priming line comprises peristaltic pump, oxygenation device, filter and the bubble removing device as power set being linked in sequence.
In such scheme, described filter two ends are provided with differential pressure pick-up, for monitoring the pressure at filter two ends, work as plugged filter, and differential pressure increases, and can, to controller signal transmission, start the warning device that controller connects.
In such scheme, described sniffer comprises the pressure sensor of the injection pressure of monitoring perfusion liquid, the gas bubble detection of the partial pressure of oxygen detector of monitoring partial pressure of oxygen, monitoring bubble removal situation.
In such scheme, described temperature control equipment comprises temperature sensor, cooling device, heater, and described temperature sensor, cooling device and heater are all connected with controller.
In such scheme, also comprise artery connector and urinary tract connector, described priming line connects renal artery by artery connector, and described kidney ureter connects urine collecting and sample window by urinary tract connector.
In such scheme, described controller is also connected with for showing human-computer interaction interface organ state and for operating.
In such scheme, also comprise waste liquid chamber, described waste liquid chamber is connected with organ storage box by check valve, also comprises that perfusion liquid gathers window, and described perfusion liquid gathers window and connects organ storage box, and the use of check valve has avoided waste liquid to backflow and situation about polluting.
In such scheme, described controller is also connected with GPS positioner.
Compared with prior art, the present invention has following beneficial effect:
One, low temperature itself also can cause damage to organ, and research is found, temperature is lower than after 20 ℃, the enzymic activity of cell has just dropped to lower standard, research finds that the subnormal temperature Perfusion preservation of 20 ℃ can provide better preservation effect, at liver, lung is all confirmed, and at kidney, our early-stage Study result is also found the activity that the perfusion of subnormal temperature perfusion lower temperature more can armour, the present invention adopts the subnormal temperature reperfusion mode of 4-32 ℃ to preserve kidney, for kidney provides a most suitable temperature environment, both can avoid energy deficiency and enzymic activity that metabolism increase causes to decline, can alleviate the damage that low temperature itself causes organ again.
Two, can preserve liquid to kidney Continuous Perfusion, lasting stable for kidney provides nutriment, meet its energy demand;
Three, the present invention, by multiple sensors and temperature control equipment are set, monitors in real time to equipment perfusion situation, and by maintaining kidney homeostasis, the condition of having created for transplanting rear organ recovery.
Four, the present invention, by reducing organ temperature, slows down cellular metabolism speed; Perfusion preservation liquid has certain soda acid buffer capacity, and whole PH meta-alkalescence; In Perfusion preservation liquid, add the means such as polyphenoils and reduced the damage to organ of acidic metabolite that anaerobic metabolism generates.
Five, isolated organ storage temperature can be adjusted, and is not limited to low temperature, thereby both can avoid energy deficiency and enzymic activity that metabolism increase causes to decline, and can alleviate the damage that low temperature itself causes organ again.
Six, by display device with perfusion is set gathers liquid and gather window and monitor at any time the preservation situation of Dispersing Monkey Kidney Cell in Tissue Culture and the variation of preserving liquid.
Seven, preservation liquid can recycle and be conducive to reduce costs.
Eight, purposes of the present invention: the 1) activity of permanently effective preservation Dispersing Monkey Kidney Cell in Tissue Culture, makes it maintain function after replanting; 2) for donor kidney different functional status and required conserving time limit when obtaining, be provided with different preservation modes; 3) by the perfusion between storage life, the damage that donor organ is suffered is repaired; 4) by perfusion parameters value, the activity of organ is assessed in the biochemical variation of perfusion liquid etc. before transplanting, assists to determine this organ classification, whether is applicable to transplant applicable receptor's scope.5) this equipment is equally applicable to large animal (dog, pig and primate) organ, carries out physiology, pharmacology related experiment.
Accompanying drawing explanation
Fig. 1 is the system block diagram of device of the present invention.
Embodiment
Below the present invention is described further:
Organ transplant is the most effectual way of the organ failure in whole latter stage for the treatment of at present, and it is particularly huge with the imbalance between supply and demand of renal transplant recipients, but the present situation of organ shortage, make us have to do one's best and make good use of each normal organ, also force us to go to attempt using some edge organs simultaneously, these edge organs are compared with standard donor organ, quality is relatively poor, in earlier stage abandoned noly transplanting development, comprised expansion standard donor organ (ECD), and DCD organ.
For normal organ, we need as much as possible before transplanting, to protect its activity; And for edge donor organ, first these organs have lived through heavier ischemic injuries, secondly, due to cause for quality, these organs are poor to the tolerance degree of ischemic injuries, more need to before transplanting, adopt suitable mode to protect.
The invention provides a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature or low temperature storage device for this reason, comprise organ storage box, fluid storage compartment, priming line, controller, described fluid storage compartment is connected with priming line input port by check valve, and priming line delivery outlet is connected with organ storage box by check valve, organ storage box is connected with priming line input port by check valve, and described organ storage box is also connected with urine urine collecting and sample window; The sniffer of setting and controller between described priming line and organ storage box, described organ storage box is provided with temperature control equipment.
In such scheme, described temperature control equipment is controlled at subnormal temperature 4-32 degree Celsius or closing temperature control device by organ storage box temperature, at organ storage box, arranges that ice cube is controlled at 0-4 degree Celsius by temperature around.
In such scheme, priming line comprises peristaltic pump, oxygenation device, filter, the bubble removing device as power set being linked in sequence.
In such scheme, described filter two ends are provided with differential pressure pick-up, for monitoring the pressure at filter two ends, work as plugged filter, and differential pressure increases, and can, to controller signal transmission, start the warning device that controller connects.
In such scheme, described sniffer comprises the pressure sensor of the injection pressure of monitoring perfusion liquid, the gas bubble detection of the partial pressure of oxygen detector of monitoring partial pressure of oxygen, monitoring bubble removal situation.
In such scheme, described temperature control equipment comprises temperature sensor, cooling device, heater, and described temperature sensor, cooling device and heater are all connected with controller.
In such scheme, also comprise artery connector and urinary tract connector, described priming line connects renal artery by artery connector, and described kidney ureter connects urine collecting and sample window by urinary tract connector.
In such scheme, described controller is also connected with for showing human-computer interaction interface organ state and for operating.
In such scheme, also comprise waste liquid chamber, described waste liquid chamber is connected with organ storage box by check valve, also comprises that perfusion liquid gathers window, and described perfusion liquid gathers window and connects organ storage box.
In such scheme, described controller is also connected with GPS positioner.
The course of work of the present invention is as follows:
Perfusion liquid is that 1L～2L is packed, is placed in fluid storage compartment, and the tube connector of sack can be connected with priming line is aseptic.
Perfusion liquid is flowed through peristaltic pump before first through a check valve, after whole filling system liquid capacity is enough, can close this check valve, and after infusion time is long, can pass through to discharge a part of waste liquid, and reopen this check valve, add new liquid, realize the object of changing liquid.
Perfusion liquid is through peristaltic pump, and the oxygenation device of flowing through, filter, bubble removing device through pressure sensor, gas bubble detection and partial pressure of oxygen detector, are monitored injection pressure, partial pressure of oxygen and the bubble removal situation of perfusion liquid before entering renal artery.
Organ (kidney) is placed in organ box, is immersed in perfusion fluid, and artery is connected by artery connector with priming line, and vein is naturally open, and perfusion liquid is flowed into by artery by the road, by vein, is flowed out in organ box.Ureter is connected with urine collecting and sample window by being positioned at the subsurface ureter connector of organ box.Perfusion liquid in organ box causes perfusion liquid sample window through conduit.Perfusion liquid sample window is the same with urine collecting and sample window, is positioned at the surface of preservation equipment, is convenient to collected specimens and detects, and in addition, urine collecting window has scale, can show urine amount.
The outlet that be provided with filter of perfusion liquid in organ box at the bottom of box, a part flow back into priming line, again passes through peristaltic pump; Another part enters in waste collection box.
embodiment mono-
When organ is preserved, temperature control equipment is controlled at subnormal temperature 4-32 degree Celsius by organ storage box temperature.Temperature control equipment comprises temperature sensor, cooling device, heater, described temperature sensor, cooling device is all connected with controller with heater, temperature sensor is distributed in organ He Huo organ locker room for one or several, the temperature information of temperature sensor Real-time Collection save set, send the temperature information collecting to central processing unit, central processing unit is processed in real time to the temperature signal collecting, obtain the temperature information of current save set and in man-machine interface, demonstrate Current Temperatures information, central processing unit is according to initialization data setting on the other hand, cooling device or the heater of controlling temperature control equipment carry out real-time PID adjusting to the temperature of organ locker room and organ box, thereby storage box temperature is controlled to subnormal temperature 4-32 degree Celsius, research finds that the subnormal temperature Perfusion preservation of 20 degree can provide better preservation effect, at liver, lung is all confirmed, and at kidney, our early-stage Study result is also found the activity that the perfusion of subnormal temperature perfusion lower temperature more can armour, the present invention adopts the normal pressure and temperature reperfusion mode of 4-32 degree to preserve kidney, for kidney provides a most suitable temperature environment, both can avoid energy deficiency and enzymic activity that metabolism increase causes to decline, can alleviate the damage that low temperature itself causes organ again.
In such scheme, priming line comprises peristaltic pump, oxygenation device, filter and the bubble removing device as power set being linked in sequence, peristaltic pump is connected with fluid reservoir, for perfusion liquid in fluid reservoir provides power, the motor of peristaltic pump is connected with central processing unit, and central processing unit is controlled peristaltic pump work.Oxygenation device can be any in membrane oxygenator, bubble oxygenator.Bubble removing device can be crossed the bubble in elimination perfusion liquid.At filter two ends, be provided with differential pressure pick-up, for monitoring the pressure at filter two ends, work as plugged filter, differential pressure increases, and can, to controller signal transmission, start the warning device that controller connects.
Sniffer comprises the pressure sensor of the injection pressure of monitoring perfusion liquid, the gas bubble detection of the partial pressure of oxygen detector of monitoring partial pressure of oxygen, monitoring bubble removal situation, gas bubble detection can be surveyed the bubble in priming line, and bubble removing device can be removed the bubble in perfusion liquid.
Also comprise artery connector and urinary tract connector, described priming line connects renal artery by artery connector, and described kidney ureter connects urine collecting and sample window by urinary tract connector, avoids contamination phenomenon.
Controller is also connected with for showing human-computer interaction interface organ state and for operating, human-computer interaction interface can real-time display system running status and temperature conditions, the mode of operation of the system that arranges that also can be artificial, the display of human-computer interaction interface can be observed the also pressure (being the perfusion change in resistance of organ) of dosage perfusion liquid.
Also comprise waste liquid chamber, described waste liquid chamber is connected with organ storage box by check valve, also comprises that perfusion liquid gathers window, and described perfusion liquid gathers window and connects organ storage box by check valve, and the situation of backflowing and polluting has been avoided in the use of check valve.
Controller is also connected with GPS positioner.
Supply unit can adopt in lithium battery, accumulator or plug any.
The course of work of the present invention is as follows:
Perfusion liquid is that 1L～2L is packed, is placed in fluid storage compartment, and the tube connector of sack can be connected with priming line is aseptic.
Perfusion liquid is flowed through peristaltic pump before first through a check valve, after whole filling system liquid capacity is enough, can close this check valve, and after infusion time is long, can pass through to discharge a part of waste liquid, and reopen this check valve, add new liquid, realize the object of changing liquid.
Perfusion liquid is through peristaltic pump, and the oxygenation device of flowing through, filter, bubble removing device through pressure sensor, gas bubble detection and partial pressure of oxygen detector, are monitored injection pressure, partial pressure of oxygen and the bubble removal situation of perfusion liquid before entering renal artery.
Organ (kidney) is placed in organ box, is immersed in perfusion fluid, and artery is connected by artery connector with priming line, and vein is naturally open, and perfusion liquid is flowed into by artery by the road, by vein, is flowed out in organ box.Ureter is connected with urine collecting and sample window by being positioned at the subsurface ureter connector of organ box.Perfusion liquid in organ box causes perfusion liquid sample window through conduit.Perfusion liquid sample window is the same with urine collecting and sample window, is positioned at the surface of preservation equipment, is convenient to collected specimens and detects, and in addition, urine collecting window has scale, can show urine amount.
embodiment bis-
Closing temperature control device when organ is preserved, arranges that ice cube is controlled at 0-4 degree Celsius by temperature around at organ storage box.
In such scheme, priming line comprises peristaltic pump, filter and the bubble removing device as power set being linked in sequence, and peristaltic pump is connected with fluid reservoir, for perfusion liquid in fluid reservoir provides power, the motor of peristaltic pump is connected with central processing unit, and central processing unit is controlled peristaltic pump work.At filter two ends, be provided with differential pressure pick-up, for monitoring the pressure of elimination filter end, work as plugged filter, differential pressure increases, and can, to controller signal transmission, start the warning device that controller connects.
Sniffer comprises the pressure sensor of the injection pressure of monitoring perfusion liquid, the gas bubble detection of monitoring bubble removal situation, and gas bubble detection can be surveyed the bubble in priming line, and bubble removing device can be removed the bubble in perfusion liquid.
Also comprise artery connector and urinary tract connector, described priming line connects renal artery by artery connector, and described kidney ureter connects urine collecting and sample window by urinary tract connector, avoids contamination phenomenon.
Controller is also connected with for showing human-computer interaction interface organ state and for operating, human-computer interaction interface can real-time display system running status and temperature conditions, the mode of operation of the system that arranges that also can be artificial, the display of human-computer interaction interface can be observed the also pressure (being the perfusion change in resistance of organ) of dosage perfusion liquid.
Also comprise waste liquid chamber, described waste liquid chamber is connected with organ storage box by check valve, also comprises that perfusion liquid gathers window, and described perfusion liquid gathers window and connects organ storage box by check valve, and the situation of backflowing and polluting has been avoided in the use of check valve.
Controller is also connected with GPS positioner.
Supply unit can adopt in lithium battery, accumulator or plug any.
The course of work of the present invention is as follows:
Perfusion liquid is that 1L～2L is packed, is placed in fluid storage compartment, and the tube connector of sack can be connected with priming line is aseptic.
Perfusion liquid is flowed through peristaltic pump before first through a check valve, after whole filling system liquid capacity is enough, can close this check valve, and after infusion time is long, can pass through to discharge a part of waste liquid, and reopen this check valve, add new liquid, realize the object of changing liquid.
Perfusion liquid is through peristaltic pump, and the filter of flowing through, bubble removing device through pressure sensor, gas bubble detection, are monitored injection pressure and the bubble removal situation of perfusion liquid before entering renal artery.
Organ (kidney) is placed in organ box, is immersed in perfusion fluid, and artery is connected by artery connector with priming line, and vein is naturally open, and perfusion liquid is flowed into by artery by the road, by vein, is flowed out in organ box.Ureter is connected with urine collecting and sample window by being positioned at the subsurface ureter connector of organ box.Perfusion liquid in organ box causes perfusion liquid sample window through conduit.Perfusion liquid sample window is the same with urine collecting and sample window, is positioned at the surface of preservation equipment, is convenient to collected specimens and detects, and in addition, urine collecting window has scale, can show urine amount.
Claims (10)
1. a Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature or low temperature storage device, it is characterized in that: comprise organ storage box, fluid storage compartment, priming line, controller, described fluid storage compartment is connected with priming line input port by check valve, and priming line delivery outlet is connected with organ storage box by check valve, organ storage box is connected with priming line input port by check valve, and described organ storage box is also connected with urine and perfusion liquid is collected and sample window; Between described priming line and organ storage box, be provided with the sniffer that is connected controller, described organ storage box is provided with temperature control equipment.
2. a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature according to claim 1 or low temperature storage device, it is characterized in that: described temperature control equipment is controlled at organ storage box temperature subnormal temperature state or the closing temperature control device of 4-32 degree Celsius, at organ storage box, arrange that ice cube is controlled at temperature the low-temperature condition of 0-4 degree Celsius around.
3. a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature according to claim 1 or low temperature storage device, is characterized in that: priming line comprises peristaltic pump, oxygenation device, filter and the bubble removing device as power set being linked in sequence.
4. a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature according to claim 3 or low temperature storage device, it is characterized in that: described filter two ends are provided with differential pressure pick-up, for monitoring the pressure at filter two ends, work as plugged filter, differential pressure increases, can, to controller signal transmission, start the warning device that controller connects.
5. a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature according to claim 1 or low temperature storage device, is characterized in that: described sniffer comprises the pressure sensor of the injection pressure of monitoring perfusion liquid, the gas bubble detection of the partial pressure of oxygen detector of monitoring partial pressure of oxygen, monitoring bubble removal situation.
6. a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature according to claim 1 or low temperature storage device, it is characterized in that: described temperature control equipment comprises temperature sensor, cooling device, heater, described temperature sensor, cooling device and heater are all connected with controller.
7. according to claim 1-3 arbitrary described a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature or low temperature storage device, it is characterized in that: also comprise artery connector and urinary tract connector, described priming line connects renal artery by artery connector, and described kidney ureter connects urine collecting and sample window by urinary tract connector.
8. a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature according to claim 4 or low temperature storage device, is characterized in that: described controller be also connected with for show organ state and for inputting the human-computer interaction interface of command operating, and be provided with the interface of derived data.
9. a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature according to claim 1 or low temperature storage device, is characterized in that: also comprise waste liquid chamber, described waste liquid chamber is connected with organ storage box by check valve
;also comprise that perfusion liquid gathers window, described perfusion liquid gathers window and connects organ storage box.
10. a kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature according to claim 1 or low temperature storage device, is characterized in that: described controller is also connected with GPS positioner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410384703.9A CN104186459A (en) | 2014-08-07 | 2014-08-07 | Sub-normal-temperature or low-temperature isolated kidney storage device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410384703.9A CN104186459A (en) | 2014-08-07 | 2014-08-07 | Sub-normal-temperature or low-temperature isolated kidney storage device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104186459A true CN104186459A (en) | 2014-12-10 |
Family
ID=52071979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410384703.9A Pending CN104186459A (en) | 2014-08-07 | 2014-08-07 | Sub-normal-temperature or low-temperature isolated kidney storage device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104186459A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686502A (en) * | 2015-02-16 | 2015-06-10 | 湖州云恩生物科技有限公司 | Safety protection enhanced organ low-temperature machine perfusion preservation device and method |
| CN105123672A (en) * | 2015-07-28 | 2015-12-09 | 东南大学 | High-precision constant-temperature perfusion instrument for isolated organs |
| CN105838665A (en) * | 2016-05-11 | 2016-08-10 | 合肥华琪生物工程有限公司 | In-vitro liver culture method |
| WO2018076567A1 (en) * | 2016-10-28 | 2018-05-03 | 中山大学附属第一医院 | Multi-organ preservation apparatus |
| CN109380213A (en) * | 2018-11-28 | 2019-02-26 | 西安交通大学医学院第附属医院 | A kind of in vitro animal viscera device for casting |
| CN109619091A (en) * | 2019-02-01 | 2019-04-16 | 陈建轩 | A kind of renal perfusion device |
| CN110786317A (en) * | 2019-11-04 | 2020-02-14 | 广州市冠流生物医学科技有限公司 | Integrated organ perfusion apparatus |
| CN111657262A (en) * | 2019-12-22 | 2020-09-15 | 西安定华电子股份有限公司 | Organ preservation device and organ preservation method |
| CN112088875A (en) * | 2020-09-28 | 2020-12-18 | 四川大学华西医院 | Organ preservation device with adjustable solution oxidation-reduction potential and implementation method thereof |
| CN112273370A (en) * | 2020-09-27 | 2021-01-29 | 中山大学附属第一医院 | Intelligent isolated organ perfusion regulation and control device and method |
| CN114514922A (en) * | 2022-03-01 | 2022-05-20 | 西安交通大学医学院第一附属医院 | Isolated heart normal atmospheric temperature mechanical perfusion device |
| CN114766470A (en) * | 2022-04-28 | 2022-07-22 | 上海健耕医药科技股份有限公司 | Low-temperature mechanical perfusion storage device and method |
-
2014
- 2014-08-07 CN CN201410384703.9A patent/CN104186459A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686502A (en) * | 2015-02-16 | 2015-06-10 | 湖州云恩生物科技有限公司 | Safety protection enhanced organ low-temperature machine perfusion preservation device and method |
| CN105123672A (en) * | 2015-07-28 | 2015-12-09 | 东南大学 | High-precision constant-temperature perfusion instrument for isolated organs |
| CN105123672B (en) * | 2015-07-28 | 2016-04-13 | 东南大学 | Isolated organ High Accuracy Constant Temperature perfusion instrument |
| CN105838665A (en) * | 2016-05-11 | 2016-08-10 | 合肥华琪生物工程有限公司 | In-vitro liver culture method |
| WO2018076567A1 (en) * | 2016-10-28 | 2018-05-03 | 中山大学附属第一医院 | Multi-organ preservation apparatus |
| CN109380213A (en) * | 2018-11-28 | 2019-02-26 | 西安交通大学医学院第附属医院 | A kind of in vitro animal viscera device for casting |
| CN109619091A (en) * | 2019-02-01 | 2019-04-16 | 陈建轩 | A kind of renal perfusion device |
| CN109619091B (en) * | 2019-02-01 | 2024-03-22 | 陈建轩 | Kidney perfusion device |
| CN110786317A (en) * | 2019-11-04 | 2020-02-14 | 广州市冠流生物医学科技有限公司 | Integrated organ perfusion apparatus |
| CN111657262A (en) * | 2019-12-22 | 2020-09-15 | 西安定华电子股份有限公司 | Organ preservation device and organ preservation method |
| CN112273370A (en) * | 2020-09-27 | 2021-01-29 | 中山大学附属第一医院 | Intelligent isolated organ perfusion regulation and control device and method |
| CN112088875A (en) * | 2020-09-28 | 2020-12-18 | 四川大学华西医院 | Organ preservation device with adjustable solution oxidation-reduction potential and implementation method thereof |
| CN112088875B (en) * | 2020-09-28 | 2023-07-25 | 四川大学华西医院 | Organ preservation device with adjustable solution oxidation-reduction potential and implementation method thereof |
| CN114514922A (en) * | 2022-03-01 | 2022-05-20 | 西安交通大学医学院第一附属医院 | Isolated heart normal atmospheric temperature mechanical perfusion device |
| CN114766470A (en) * | 2022-04-28 | 2022-07-22 | 上海健耕医药科技股份有限公司 | Low-temperature mechanical perfusion storage device and method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104186459A (en) | Sub-normal-temperature or low-temperature isolated kidney storage device | |
| CN205124849U (en) | Separation liver normal atmospheric temperature or low control by temperature change temperature perfusion device | |
| CN105379707B (en) | Liver normal temperature irrigates repair system | |
| CN105660604B (en) | A kind of fresh dissected dog's liver system and fresh dissected dog's liver method | |
| Fondevila et al. | Superior preservation of DCD livers with continuous normothermic perfusion | |
| ES2250119T3 (en) | PROCEDURES AND MEANS FOR EXTRACORPORAL ORGAN PERFUSION. | |
| CN103719075B (en) | Isolated organ preserving device and preserving method thereof | |
| CA2620328C (en) | Machine perfusion of tissue grafts for transplantation | |
| CN104705288B (en) | Subnormal temperature transplant organ vitro intelligent supports System and method for | |
| CN109717184B (en) | Mechanical perfusion system for in-vitro double-kidney preservation | |
| CN209473458U (en) | A kind of liver room temperature machine perfusion system | |
| Maione et al. | Porcine isolated liver perfusion for the study of ischemia reperfusion injury: a systematic review | |
| Monbaliu et al. | Hypothermic machine perfusion of the liver: is it more complex than for the kidney? | |
| CN112244007A (en) | Intelligent isolated organ long-term maintenance device | |
| CN103548814A (en) | Non-programmable-controlled cooling cryopreservation method and protection agent for hematopoietic stem cells at minus 80DEG C | |
| CN115777690A (en) | Method for cryopreservation of umbilical cord blood and peripheral blood, and solution for cryopreservation | |
| CN104686502A (en) | Safety protection enhanced organ low-temperature machine perfusion preservation device and method | |
| CN209546716U (en) | A kind of toy organ room temperature machine perfusion system | |
| Kuan et al. | A study of normothermic hemoperfusion of the porcine pancreas and kidney | |
| Yoshikawa et al. | Evaluation using an isolated reperfusion model for porcine liver donated after cardiac death preserved with oxygenated hypothermic machine perfusion | |
| CN204104610U (en) | A kind of Dispersing Monkey Kidney Cell in Tissue Culture subnormal temperature or low temperature storage device | |
| CN215583022U (en) | Normal-temperature blood mechanical perfusion system suitable for rat donor heart | |
| CN111480644A (en) | Normal-temperature mechanical perfusion combined with gene modification for repairing small animal organ system by stem cells | |
| CN206978567U (en) | A kind of machine perfusion medicament slow release pump installation | |
| CN205547132U (en) | Liver normal atmospheric temperature fills repair system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141210 |