CN111558008A

CN111558008A - Lotus-atractylodes-rhizome fat-reducing navel patch, preparation method and application

Info

Publication number: CN111558008A
Application number: CN202010487564.8A
Authority: CN
Inventors: 王京龙; 郑丹丹; 张立华; 吕慧
Original assignee: Zaozhuang University
Current assignee: Zaozhuang University
Priority date: 2020-06-02
Filing date: 2020-06-02
Publication date: 2020-08-21

Abstract

The invention belongs to the technical field of weight-losing and lipid-lowering medicines, and discloses a lotus leaf weight-losing and lipid-lowering navel patch, a preparation method and application thereof, wherein raw medicinal materials are mixed according to a proportion and crushed, and the obtained powder is sieved by a 80-mesh sieve to obtain medicinal material powder; reflux-extracting part of the obtained medicinal powder to obtain crude extract; purifying the crude extract by using macroporous adsorption resin to prepare a total flavone active extract; dissolving the total flavone active extract and azone in a certain amount of anhydrous ethanol according to a certain proportion to obtain a medicine-containing mixed ethanol solution; dissolving mixed fatty glyceride and lanolin at 60 deg.C, adding medicated ethanol solution after dissolving completely, and mixing completely; dripping into blank patch according to a volume of 1ml per patch, condensing, molding, and packaging. The lotus leaf, rhizoma atractylodis, weight-losing and lipid-lowering navel patch prepared by the invention has certain safety, certain formability and better appearance state. The animal skin test shows that no obvious skin irritation reaction exists.

Description

Lotus-atractylodes-rhizome fat-reducing navel patch, preparation method and application

Technical Field

The invention belongs to the technical field of weight-losing and lipid-lowering medicines, and particularly relates to a lotus-atractylodes weight-losing and lipid-lowering navel patch, a preparation method and application thereof.

Background

Currently, the current state of the art commonly used in the industry is such that: at present, health care products or medicines for losing weight and reducing fat are mainly taken orally or injected, such as tablets, capsules, soft capsules, oral liquid or injection administration medicines. With the improvement of modern living standard, people pay more and more attention to their health, but diseases caused by obesity are not ignored, and some obese people have various diseases, such as hypertension, hyperlipidemia and other diseases, which seriously affect human health. Therefore, the development of drugs with the effects of reducing blood pressure, losing weight and reducing blood fat has been paid attention to by people. The bioavailability of oral administration is low, and the bioavailability of injection administration is high, but the administration is inconvenient. The navel administration is directly carried out through the navel by a penetration enhancer, the bioavailability is high, the use is convenient, and the effect is quick.

In summary, the problems of the prior art are as follows: the existing medicines with the effects of reducing blood pressure, losing weight and reducing blood fat mostly adopt an oral or injection mode, so that the preparation process is complex and the stimulation to the gastrointestinal tract is large; moreover, the smell of some medicinal materials has certain irritation, which is not beneficial for patients to take.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a lotus leaf weight-losing and lipid-lowering navel patch, a preparation method and application thereof.

The invention is realized in this way, a preparation method of a lotus leaf, rhizoma atractylodis, weight-losing and lipid-lowering navel patch, which specifically comprises the following steps:

step one, mixing raw medicinal materials according to a certain proportion, processing the raw medicinal materials, putting the processed raw medicinal materials into a grinder for grinding, and sieving the obtained powder with a 80-mesh sieve to obtain medicinal material powder;

step two, preparing a crude extract of a sample from part of prepared medicinal material powder by using a reflux extraction method;

purifying the crude extract by using macroporous adsorption resin; the total flavone active extract is prepared without using traditional Chinese medicine extract, so that the administration dosage is reduced, and the drug-loading rate of actual drug-effect substances is improved;

dissolving the total flavone active extract and azone obtained in the step three in a certain proportion into a certain amount of absolute ethanol to prepare a medicine-containing mixed ethanol solution; penetration enhancer for improving transdermal absorption effect and bioavailability; 1g of total flavone extract and 2ml of absolute ethyl alcohol;

step five, mixing the fatty glyceride and the lanolin according to a proportion, dissolving at 60 ℃, weighing the mixed ethanol solution containing the medicine in the step four according to a proportion, pouring the mixed fatty glyceride and the lanolin into the mixed fatty glyceride and the lanolin according to a proportion, and fully and uniformly mixing;

and step six, dripping the blank paste into the navel paste according to the volume of 1ml of each paste when the paste is hot, condensing, forming and packaging to obtain the lotus leaf weight-losing and lipid-lowering navel paste.

Further, in the first step, the raw material mixing ratio specifically includes: 9g of lotus leaf, 9g of fried bighead atractylodes rhizome, 6g of scorched hawthorn fruit, 6g of coix seed, 3g of dried orange peel and 3g of cassia seed.

Further, in the second step, the preparation of the sample solution by the reflux extraction method specifically comprises:

firstly, fully and uniformly mixing medicinal fine powder according to a certain proportion, putting 70.0g of the uniformly mixed medicinal fine powder into a round-bottomed flask, adding 20 times of 80% ethanol, carrying out reflux extraction on the mixture for 2 times at 90 ℃ in a water bath kettle for two hours in total, combining the two crude extraction solutions, centrifuging to obtain a filtrate, fixing the volume, and shaking uniformly to obtain an extraction solution;

secondly, decompressing and concentrating the extracting solution in a rotary evaporator to obtain a crude extract;

further, in the third step, the purification method specifically comprises:

loading a sample with the mass concentration of pH 3.5 and 0.75mg/mL, the loading amount of resin being 4.4mL/g, the volume flow of the loaded sample being 1.5BV/h, sequentially eluting with 2.0BV of water and 3.0BV of 60% ethanol, concentrating the collected eluent in a rotary evaporator under reduced pressure to a certain volume, transferring the eluent to an evaporation dish, evaporating the eluent to dryness on a water bath, finally transferring the eluent to a reduced-pressure drying oven, and drying the eluent for 8 to 12 hours.

Further, in the fifth step, the dosages of the mixed fatty glyceride, the lanolin and the absolute ethyl alcohol are as follows: the dosage of the mixed fatty glyceride is 2g, the dosage of the lanolin is 2g, and the dosage of the absolute ethyl alcohol is 1 mL.

The invention also aims to provide the lotus leaf, rhizoma atractylodis, weight-losing and lipid-lowering navel patch prepared by the preparation method of the lotus leaf, rhizoma atractylodis, weight-losing and lipid-lowering navel patch.

Further, the drug loading of the lotus leaf weight-losing and lipid-lowering navel patch is 10%.

In summary, the advantages and positive effects of the invention are: the lotus leaf, rhizoma atractylodis, weight-losing and lipid-lowering navel patch prepared by the invention has certain safety, certain formability and better appearance state. The animal skin test shows that no obvious skin irritation reaction exists. The lotus leaf tea takes the lotus leaf as a monarch drug, the bighead atractylodes rhizome as a ministerial drug, the hawthorn and the tangerine peel as adjuvant drugs, and the cassia seed and the coix seed as conductant drugs, and has the effects of losing weight, reducing blood fat and improving the functions of intestines and stomach, so that the effect of losing weight without damaging the body resistance is achieved.

The invention avoids the taking modes of oral taking, injection and the like, adopts the navel patch, and has no stimulation to the gastrointestinal tract; obtaining the slow release and controlled release effects; avoid hepatic first pass effect; the use is convenient, and the compliance of patients is good; high safety and easy removal.

The invention adopts a reflux extraction method to extract the weight-losing and lipid-lowering prescription of rhizoma atractylodis macrocephalae, and adopts macroporous adsorption resin to purify the extracting solution, and finally obtains the active extract of the general flavone as the raw material medicine of the effective component in the navel patch; the dosage of semisynthetic fatty glyceride, the dosage of absolute ethyl alcohol and the dosage of lanolin in the blank matrix of the navel plaster are investigated, and the matrix proportion of the navel plaster is optimized. Compared with the traditional Chinese medicine, the navel patch is more convenient to directly decoct and take, has wide market prospect, and plays a certain promoting role in promoting the application of the navel patch in the aspect of medical care and promotion and the popularization of the traditional Chinese medicine in the world.

Drawings

Fig. 1 is a flow chart of a preparation method of a hilum plaster for reducing weight and fat in lotus technique according to an embodiment of the invention.

Fig. 2 is a schematic diagram of a rutin standard curve provided by an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Aiming at the problems in the prior art, the invention provides a lotus leaf weight-losing and lipid-lowering navel patch, a preparation method and application thereof, and the invention is described in detail with reference to the accompanying drawings.

As shown in fig. 1, the preparation method of the navel patch for losing weight and reducing fat provided by the embodiment of the invention comprises the following steps:

s101, mixing the raw medicinal materials according to a certain proportion, processing the raw medicinal materials, putting the processed raw medicinal materials into a grinder to grind, and sieving the obtained powder with a 80-mesh sieve to obtain medicinal material powder.

S102, taking part of the prepared medicinal material powder, and preparing a crude extract by using a reflux extraction method.

S103, purifying the sample liquid by using macroporous adsorption resin to obtain the total flavone active extract.

And S104, dissolving the total flavone active extract and the azone obtained in the step S103 in a certain amount of absolute ethanol according to a certain proportion to prepare a medicine-containing mixed ethanol solution.

And S105, mixing the fatty glyceride and the lanolin according to the proportion, dissolving at 60 ℃, pouring the medicine-containing mixed ethanol solution into the mixed fatty glyceride and the lanolin according to the proportion, and fully and uniformly mixing.

S106, dripping the blank paste into the paste when the paste is hot according to the volume of 1ml of each paste, condensing, forming and packaging to obtain the lotus leaf weight-losing and lipid-lowering navel paste.

In a preferred embodiment of the present invention, in step S101, the mixing ratio of the raw materials specifically includes:

9g of lotus leaf, 9g of fried bighead atractylodes rhizome, 6g of scorched hawthorn fruit, 6g of coix seed, 3g of dried orange peel and 3g of cassia seed.

In a preferred embodiment of the present invention, in step S102, the preparing of the sample solution by the reflux extraction method specifically includes:

firstly, fully and uniformly mixing medicinal fine powder in a mortar according to a certain proportion, putting 70.0g of the uniformly mixed medicinal fine powder into a round-bottomed flask, adding 20 times of 80% ethanol, carrying out reflux extraction on the mixture for 2 times at 90 ℃ in a water bath kettle for two hours in total, combining the two crude extract solutions, centrifuging to obtain a filtrate, fixing the volume, and shaking uniformly to obtain an extract.

And secondly, decompressing and concentrating the extracting solution in a rotary evaporator to prepare a crude extract.

In a preferred embodiment of the present invention, in step S103, the method for purifying the sample solution specifically includes: loading a sample with the mass concentration of pH 3.5 and 0.75mg/mL, the loading amount of resin being 4.4mL/g, the volume flow of the loaded sample being 1.5BV/h, sequentially eluting with 2.0BV of water and 3.0BV of 60% ethanol, concentrating the collected eluent in a rotary evaporator under reduced pressure to a certain volume, transferring the eluent to an evaporation dish, evaporating the eluent to dryness on a water bath, finally transferring to a reduced-pressure drying oven, and drying for 8-12 hours.

In the preferred embodiment of the present invention, in step S104, the total flavone extract is 1g, and the absolute ethanol is used in an amount of 2 ml.

In a preferred embodiment of the present invention, in step S105, the mixed fatty glyceride, lanolin, and absolute ethanol are used in the following amounts: the dosage of the mixed fatty glyceride is 2g, the dosage of the lanolin is 2g, and the dosage of the absolute ethyl alcohol is 1 mL. The drug loading of the lotus leaf weight-reducing and lipid-lowering navel patch provided by the embodiment of the invention is 10%.

The application method of the navel patch for losing weight and reducing fat provided by the embodiment of the invention specifically comprises the following steps:

(1) the navel was wiped with warm water to remove the dirt and the surface of the navel was dried.

(2) The paster is uncovered, and the ointment is aligned with the navel and is pasted on the navel, so that the medicine is fully contacted with the skin.

(3) Rub the hands with the heat, apply the palm to the navel, and gently soften for three minutes.

The technical effects of the present invention will be described in detail with reference to pharmacology.

The lotus leaf in the lotus leaf weight-reducing and lipid-lowering formula is the leaf of lotus of the family Nymphaeaceae. The lotus leaves contain various alkaloids and flavonoids, and modern researches show that: the total flavonoids in folium Nelumbinis has effects of reducing weight and blood lipid, inhibiting bacteria, resisting oxidation, resisting virus, and scavenging free radicals. The Atractylodis rhizoma is dry rhizome of Atractylodes macrocephala Koidz of Compositae, is bitter, sweet and warm in nature, and has effects of invigorating spleen, invigorating qi, eliminating dampness, promoting diuresis, arresting sweating, and preventing miscarriage. The fructus crataegi is dry mature fruit of Crataegus pinnatifida bge or Crataegus pinnatifida bge of Rosaceae, has sour, sweet and slightly warm nature and taste, and enters spleen, stomach and liver channels. Has the effects of promoting digestion, invigorating stomach, promoting qi circulation, removing blood stasis, eliminating turbid pathogen, and reducing blood lipid, and can be used for promoting digestion, and stimulating appetite. The hawthorn fruit contains a large amount of flavonoid compounds and minerals such as sodium, calcium, magnesium, potassium and the like, has better curative effects on reducing blood pressure and blood fat, can promote vasodilatation and increase coronary blood flow, and has the effects of reducing blood pressure, reducing blood fat, increasing heart blood flow and the like on a human body.

The navel therapy method of traditional Chinese medicine belongs to one of the traditional Chinese medicine external treatment methods, and takes a navel (Shenque acupoint) as a medicine application or stimulation part to stimulate channel qi, dredge channels and collaterals, promote qi and blood circulation, and regulate the functions of internal organs and yin and yang of a human body, thereby achieving the purpose of preventing and treating diseases. The navel therapy is a treasure in the traditional Chinese medicine and has been used for thousands of years. The navel treatment dosage form comprises powder, paste (ointment), plaster, patch, bag agent, pellicle, liquid preparation, etc. The navel patch researched by the invention is one of the patches.

The technical effects of the present invention will be further described with reference to the following specific examples.

Example 1:

1. materials and instruments

1.1 test materials

The medicinal materials are purchased from Liyukun traditional Chinese medicine clinics, and are identified by King Jinglong teachers: the lotus leaf is dry leaf of lotus of Nymphaeaceae, the white atractylodes rhizome is dry rhizome of white atractylodes rhizome of Compositae, the hawthorn is dry mature fruit of Chinese hawthorn or hawthorn of Rosaceae, the dried tangerine peel is dry mature pericarp of tangerine of Rutaceae and its cultivar, the coix seed is dry mature kernel of cereal plant coix seed, the cassia seed is dry mature seed of cassia seed or cassia tora of Leguminosae, wherein the white atractylodes rhizome is self-made fried white atractylodes rhizome shaped like white atractylodes rhizome slice, the surface is yellowish brown, the focal spot is occasionally seen, the flavor is slightly scorched, the hawthorn is self-made scorched hawthorn, the surface is scorched brown, the interior is yellowish brown, and the flavor is scorched.

Materials and reagents used in the experiments are shown in table 1.

TABLE 1 materials and reagents

1.2 instruments and devices

The instruments and equipment are shown in Table 2.

TABLE 2 instruments and apparatus

2. Test method

2.1 preparation and purification of sample liquid in weight-reducing and lipid-lowering prescription from Heshu

2.1.1 preparation of sample solutions

A reflux extraction method: the preparation method comprises the steps of firstly processing raw medicinal materials, then putting the raw medicinal materials into a grinder for grinding, sieving the obtained powder with a 80-mesh sieve, then fully and uniformly mixing the medicinal fine powder in a mortar according to a certain proportion, putting 70.0g of the uniformly mixed medicinal fine powder into a round-bottomed flask, adding 20 times of 80% ethanol, carrying out reflux extraction on the mixture for 2 times on a water bath kettle at 90 ℃, combining the two crude extract solutions for two hours in total, centrifuging to obtain filtrate, fixing the volume, and shaking uniformly to obtain the extract. Concentrating the extractive solution under reduced pressure in a rotary evaporator, and pouring out the concentrated solution to obtain crude extract.

2.1.2 purification of sample liquid

Purifying with macroporous adsorption resin: the optimal conditions are determined by pre-experiments to be pH 3.5, the sample loading with the mass concentration of 0.75mg/mL is carried out, the sample loading amount of the resin is 4.4mL/g, the volume flow of the sample loading is 1.5BV/h, the elution is sequentially carried out by using 2.0BV of water and the elution is carried out by using 3.0BV of 60 percent ethanol, the collected eluent is decompressed and concentrated to a certain volume by a rotary evaporator, then transferred to an evaporating dish and evaporated to dryness in a water bath, and finally transferred to decompression, drying and baking for 8-12 hours. The flavone powder purified by the D-101 type resin has the purification rate reaching 71.6 percent and the total flavone mass fraction reaching 66.80 percent and is used as the raw material medicine of the navel patch.

2.2 determination of Total Flavonoids in sample liquid in weight-reducing and lipid-lowering prescription from Heshu

2.2.1 preparation of control solutions

Accurately weighing 5.0mg of dried rutin control to constant weight, placing in 25mL volumetric flask, adding appropriate amount of methanol, ultrasonic treating to dissolve, cooling, adding methanol to scale, shaking, and refrigerating to obtain control solution (0.2 mg/mL).

2.2.2 preparation of Standard Curve

Precisely absorbing rutin reference substances of 0, 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0mL, respectively placing in a 25mL measuring flask, respectively adding 30% ethanol to 6mL, adding 5% sodium nitrite solution of 1mL, uniformly mixing, placing for 6min, adding 10% aluminum nitrate solution of 1mL, uniformly mixing, placing for 6min, adding sodium hydroxide solution of 10mL, adding 30% ethanol to the scale, uniformly mixing, placing for 15min, and measuring the absorbance at the wavelength of 500nm by using an ultraviolet spectrophotometer. A standard curve was plotted with the absorbance A as the ordinate and the mass concentration C (mg/mL) as the abscissa.

2.2.3 determination of Total Flavonoids content

Precisely absorbing 1mL of sample liquid, placing the sample liquid in a 25mL volumetric flask, measuring the absorbance according to the method from 'adding water to 6mL and adding 5% sodium nitrite to 1 mL' according to a 3.2.2 method, substituting the absorbance into a standard curve to obtain the concentration of the sample solution, and calculating to obtain the test solution.

2.3 preparation of blank plaster of HESHU weight-reducing and lipid-lowering navel plaster

2.3.1 orthogonal design experiment

According to the preliminary experiment research, determining mixed fatty glyceride as a matrix, lanolin as a tackifier, absolute ethyl alcohol as a dispersing agent, selecting the dosage of the mixed fatty glyceride, the lanolin and the absolute ethyl alcohol as an investigation factor, and adopting L₉(3⁴) Orthogonal tables were tested and the protocol is shown in table 3.

TABLE 3 factor level table

2.3.2 preparation method and Scoring Standard

The preparation method comprises the following steps: weighing a certain amount of azone, putting the azone into a certain amount of absolute ethyl alcohol, weighing a certain amount of mixed fatty glyceride and lanolin, putting the mixed fatty glyceride and lanolin into a beaker, melting the mixed fatty glyceride and lanolin in a water bath kettle at 60 ℃, weighing a certain amount of absolute ethyl alcohol, adding the absolute ethyl alcohol into the mixed fatty glyceride and lanolin, fully and uniformly mixing the mixture after the mixed fatty glyceride and lanolin are completely melted, pouring the mixture on a glass flat plate while the mixture is hot, and observing the state of the mixture.

Grading standard: the paste was rated according to the state of dispersion, fluidity, viscosity and appearance of the paste on a glass plate, and was rated as 1. The dispersion state is given 0-0.3 minutes from weak to strong, the fluidity is given 0-0.2 minutes from strong to weak, the viscosity is given 0-0.3 minutes from weak to strong, and the appearance is given 0-0.2 minutes according to the integrity difference. The combined results were scored according to these 4 criteria.

3. Results and analysis

3.1 results of plotting rutin Standard Curve

TABLE 4 plotting of Standard curves

Concentration (mg/ml)	0.008	0.016	0.024	0.032	0.040	0.048
							Absorbance A	0.100	0.183	0.268	0.350	0.436	0.522

Linear analysis was performed on the data obtained in table 4 and fig. 2, and the regression equation between the rutin concentration (mg/mL) and the absorbance (a) was obtained as follows: 10.5393x +0.0147, R²＝0.9999。

The concentration of the rutin control substance is in a good linear relation within the range of 0.008-0.048 mg/mL as can be seen from a regression equation and a standard curve.

3.2 preparation and evaluation of HESHU navel patch for reducing weight and blood lipid

3.2.1 results of orthogonal experiments

The results of the orthogonality test are shown in Table 5.

TABLE 5 results of orthogonal experiments

TABLE 6 analysis of variance

As can be seen from the visual analysis, the sequence influencing the formulation forming factors is C>B>A; the variance result shows that C has influence on the forming of the navel patch; the optimal prescription of blank matrix is determined by comprehensive consideration as A₂B₂C₁Preferably, the mixed fatty glyceride is 2g, and lanolin is 2gThe amount of absolute ethyl alcohol is 1 mL.

3.2.2 preparation of weight-losing and lipid-lowering navel patch

Preparing a plaster according to the optimal screened blank matrix prescription and the drug loading rate of 10 percent: dissolving a certain amount of refined total flavone active extract and azone in a certain amount of absolute ethyl alcohol according to a certain proportion, putting a certain amount of mixed fatty glyceride and lanolin in a beaker, melting the mixed fatty glyceride and lanolin in a water bath kettle at 60 ℃, measuring a certain amount of absolute ethyl alcohol after the mixed fatty glyceride and lanolin are completely melted, pouring the absolute ethyl alcohol into the mixed fatty glyceride and lanolin, fully mixing the mixed fatty glyceride and lanolin uniformly, and pouring the mixture into a blank paste while the mixture is hot to obtain the weight-reducing and lipid-lowering navel paste for the lotus art. The best preparation prescription obtained in this example is to prepare 10 pieces of navel plaster (each plaster contains about 0.09g of medicine).

Use introduction: 1. wiping the navel with warm water, removing dirt, and finally wiping the surface of the navel; 2. uncovering the paster, and aligning the ointment to the navel to make the medicine fully contact with the skin; 3. rub the hands with the heat, apply the palm to the navel, and gently soften for three minutes.

3.2.3 evaluation of Lotus technique fat-reducing navel plaster

And (3) optimal process verification: the optimal preparation process obtained by the test is repeated for 3 times, and the prepared lotus weight-losing and lipid-lowering navel patch is comprehensively evaluated, and the results are all in line with the expected effect.

Skin irritation test: selecting one healthy rabbit, unhairing two sides of the spinal column of the back before administration, and checking the integrity of the skin after unhairing, wherein the skin is ensured to be intact and not injured. The prepared navel patch is attached to the skin of a rabbit and is taken down for 2, 4, 6, 8, 10, 12 and hours respectively to observe the skin condition of the rabbit, the skin allergy and red swelling conditions are not found, and the patch is proved to not cause irritation to the skin and has certain use safety.

4. Results

4.1 determination of optimum Process conditions

The matrix of the lotus leaf weight-losing and lipid-lowering navel patch is optimized, the formability of the whole navel patch is inspected, and test results show that when the dosage of the mixed fatty glyceride is 2g, the dosage of the lanolin is 2g, and the dosage of the absolute ethyl alcohol is 1mL, the integrity of the patch is good, and the patch can be used for preparing the navel patch. Moreover, the stimulation condition of the navel patch to the skin is investigated, the skin is not stimulated, and the prepared navel patch is proved to have certain safety and certain market development and use prospects.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims

1. A preparation method of a lotus leaf, rhizoma atractylodis and tripe navel paste for losing weight and reducing fat is characterized in that the preparation method of the lotus leaf, rhizoma atractylodis and tripe navel paste for losing weight and reducing fat comprises the following steps:

step one, mixing raw medicinal materials according to a ratio, processing the raw medicinal materials, then putting the processed raw medicinal materials into a grinder for grinding, and sieving the obtained powder to obtain medicinal material powder;

step two, taking the prepared medicinal material powder to prepare a crude extract by a reflux extraction method;

purifying the crude extract by using macroporous adsorption resin to prepare an active extract;

step four, dissolving the active extract and the azone obtained in the step three in a certain amount of absolute ethanol according to a certain proportion to prepare a medicine-containing mixed ethanol solution;

dissolving a certain amount of mixed fatty glyceride and lanolin at 60 ℃, adding the medicine-containing ethanol solution according to a proportion after the mixed fatty glyceride and lanolin are completely dissolved, and fully and uniformly mixing;

2. The preparation method of the hilum plaster with holy surgery weight-losing and lipid-lowering functions as claimed in claim 1, wherein in the first step, the raw materials are mixed according to the following ratio: 9g of lotus leaf, 9g of fried bighead atractylodes rhizome, 6g of scorched hawthorn fruit, 6g of coix seed, 3g of dried orange peel and 3g of cassia seed; the resulting powder was sieved through a 80 mesh sieve.

3. The method for preparing the hilum plaster with holy surgery weight-losing and lipid-lowering functions as claimed in claim 1, wherein in the second step, the preparation of the sample solution by the reflux extraction method comprises the following steps:

firstly, fully and uniformly mixing medicinal fine powder in a mortar according to a proportion, putting 70.0g of the uniformly mixed medicinal fine powder into a round-bottomed flask, adding 20 times of 80% ethanol, carrying out reflux extraction on the mixture for 2 times at 90 ℃ in a water bath kettle for two hours in total, combining the two crude extract solutions, centrifuging to obtain a filtrate, fixing the volume, and shaking uniformly to obtain an extract;

and secondly, carrying out reduced pressure concentration on the extracting solution in a rotary evaporator to obtain a crude extract of the Heshu weight-losing and lipid-lowering prescription.

4. The method for preparing the hilum plaster with holy surgery weight-losing and lipid-lowering functions as claimed in claim 1, wherein in the third step, the method for purifying the sample liquid comprises the following steps: loading a sample with the mass concentration of 0.75mg/mL and the pH value of 3.5, wherein the loading amount of the resin is 4.4mL/g, the volume flow of the loaded sample is 1.5BV/h, sequentially eluting with 2.0BV of water and 3.0BV of 60% ethanol, concentrating the collected eluent under reduced pressure in a rotary evaporator, transferring the concentrated eluent to an evaporation dish, evaporating the eluent on a water bath kettle to dryness, finally transferring the eluent to a reduced-pressure dryer, and drying the eluent for 8 to 12 hours.

5. The method for preparing the HESHU JIANFEI JIANGZHI MAIQI Patch as claimed in claim 1, wherein,

1g of total flavone extract in the fourth step and 2ml of absolute ethyl alcohol;

in the fifth step, the dosages of the mixed fatty glyceride, the lanolin and the absolute ethyl alcohol are as follows: the dosage of the mixed fatty glyceride is 2g, the dosage of the lanolin is 2g, and the dosage of the absolute ethyl alcohol is 1 mL; putting the mixed fatty glyceride and the lanolin into a beaker, and melting the mixed fatty glyceride and the lanolin in a water bath kettle at the temperature of 60 ℃;

dissolving the mixed fatty glyceride and the lanolin at 60 ℃, adding the medicine-containing ethanol solution according to the proportion after the mixed fatty glyceride and the lanolin are completely dissolved, and fully and uniformly mixing;

and sixthly, dripping the blank paste into the patch while the patch is hot according to the volume of 1ml per patch, condensing, forming and packaging to obtain the lotus leaf weight-losing and lipid-lowering navel patch.

6. A lotus leaf, rhizoma atractylodis and bellied navel paste with weight losing and lipid lowering functions, which is prepared by the preparation method of the lotus leaf, rhizoma atractylodis and bellied navel paste with weight losing and lipid lowering functions of any one of claims 1 to 5.

7. The hilum plaster for holy surgery to reduce weight and fat of claim 6, wherein the drug-loading rate of the hilum plaster for holy surgery to reduce weight and fat is 10%.

8. The use of the navel patch for losing weight and reducing fat of lotus leaf as claimed in claim 6 in weight-reducing medicine.

9. The use of the navel patch for weight losing and lipid lowering according to claim 6 in a lipid lowering medicine.

10. The use of the navel patch for losing weight and reducing fat of lotus leaf as claimed in claim 6 in a medicine for reducing blood pressure.