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CN111349143A - Protein dialysis device and dialysis method - Google Patents

Protein dialysis device and dialysis method Download PDF

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Publication number
CN111349143A
CN111349143A CN202010163179.8A CN202010163179A CN111349143A CN 111349143 A CN111349143 A CN 111349143A CN 202010163179 A CN202010163179 A CN 202010163179A CN 111349143 A CN111349143 A CN 111349143A
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China
Prior art keywords
dialysis
block
dialysate
main body
protein
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Withdrawn
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CN202010163179.8A
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Chinese (zh)
Inventor
雍金贵
李森
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Anhui Global Gene Technology Co ltd
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Anhui Global Gene Technology Co ltd
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Priority to CN202010163179.8A priority Critical patent/CN111349143A/en
Publication of CN111349143A publication Critical patent/CN111349143A/en
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/34Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Water Supply & Treatment (AREA)
  • External Artificial Organs (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

The invention discloses a protein dialysis device and a dialysis method, wherein a fixed bottom block, a spring and a vibrator are arranged in the device and used in a matched manner, so that dialysate can better permeate a dialysis membrane, the efficiency and the quality of dialysis are effectively improved, the problems of position deviation and toppling of a dialysis main body in the shaking process can be avoided due to the matched use of the fixed column, the spring and an elastic supporting column, the introduced liquid can be uniformly sprinkled on the dialysis membrane through a shunt through the arranged shunt, the dialysis speed and the dialysis quality can be effectively increased, and the problems that the dialysis membrane is damaged due to impact and the dialysis efficiency is low due to the fact that the dialysis liquid is not shunted by the conventional equipment are solved; the cooperation through the first dialysis membrane that sets up, second dialysis membrane, fixed lug and solid fixed ring is used, and the dialysate is from last down by gravity and shaking the effect down for the effect of dialysate layering is better, has solved the problem of dialysate separation back liquid reflux in the past.

Description

Protein dialysis device and dialysis method
Technical Field
The invention relates to the technical field of protein dialysis equipment, in particular to a protein dialysis device and a dialysis method.
Background
Dialysis is a method of achieving separation by utilizing the property that small molecular substances can pass through a semipermeable membrane in a solution, while large molecular substances cannot pass through the semipermeable membrane. The protein can be dialyzed to remove inorganic salt, monosaccharide, disaccharide and other impurities, or else, macromolecular impurities can be retained in the semipermeable membrane, and micromolecular substances enter the solution outside the membrane through the semipermeable membrane for separation and refining, so that the specification relation between the success of dialysis and the dialysis membrane is extremely large, and the existing protein dialysis device has certain defects.
Patent document (CN201721815116) discloses a protein dialysis device and system, which includes a driving device and a dialysis rotating frame: the utility model discloses a protein dialysis system, including protein dialysis device, the utility model discloses both can avoid using magnetic stirrers, the sample leaks, intertwine between a plurality of dialysis bags to and the dialysis bag can appear the condition of damage and take place, can accelerate the penetration of micromolecular protein or other impurity in the dialysis bag again, reach the purpose of protein purification; the device is dialyzing through the dialysis bag, makes the separating medium backward flow of dialysis cause the dialysis quality low easily, and the device accelerates the speed of dialysis through the pivoted mode moreover, and the dislysate produces certain pressure to the dialysis bag under the effect of gravity and centrifugal force, probably leads to the dialysis membrane in the dialysis bag to cause the damage, and the device has defects such as dialysis efficiency is low and the dialysis quality is poor.
Disclosure of Invention
The invention aims to provide a protein dialysis device, and the technical problems to be solved are as follows:
(1) according to the invention, through the matched use of the fixed bottom block, the spring and the vibrator, the dialysis main body on the fixed bottom block is shaken through the left-right vibration of the vibrator, so that dialysate can better permeate a dialysis membrane, the efficiency and the quality of dialysis are effectively improved, and the matched use of the fixed column, the spring and the elastic support column can prevent the dialysis main body from shifting and toppling in the shaking process, so that the problem that the dialysis speed is improved in the prior art through a stirring mode, but cells of the dialysate are easily damaged in the stirring mode is solved;
(2) according to the invention, the introduced liquid can be uniformly sprinkled on the dialysis membrane through the flow divider, so that the dialysis speed and the dialysis quality can be effectively increased, and the problems that the dialysis membrane is damaged due to impact and the dialysis efficiency is low because the dialysis liquid is not divided by the conventional equipment are solved;
(3) according to the invention, the first dialysis membrane, the second dialysis membrane, the fixing lug and the fixing ring are matched for use, so that the dialysis quality can be effectively improved when dialysate is dialyzed by the two layers of dialysis membranes, and the layering effect of the dialysate is better under the action of gravity and shaking force from top to bottom, so that the problem of poor dialysis quality caused by liquid backflow after dialysate separation in the past is solved.
The purpose of the invention can be realized by the following technical scheme:
a protein dialysis device comprises a dialysis main body, wherein a material guide pipe is embedded in the dialysis main body at a position close to the upper part, an inner shell is fixedly arranged in the dialysis main body at a position close to the middle, a shunt is movably connected to the position close to the upper part of the inner surface of the inner shell, a first dialysis membrane and a second dialysis membrane are fixedly arranged in the inner shell, the first dialysis membrane is positioned above the second dialysis membrane, the lower surface of the dialysis main body is fixedly provided with a supporting block, a first liquid guide port and a first clamping ring are embedded at the position, close to the upper part, of the outer surface of the dialysis main body, the first liquid guide port is positioned above the first clamping ring, a second liquid guide port and a second clamping ring are embedded in the position, close to the middle, of the outer surface of the dialysis main body, the second drainage port is positioned above the second clamping ring, and a third drainage port is embedded in the position, close to the lower part, of the outer surface of the dialysis main body.
Furthermore, a fixing column is fixedly mounted on one side of the dialysis main body, a supporting clamping block is fixedly mounted on one side of the fixing column close to the dialysis main body, and the fixing column is movably connected with the dialysis main body through the supporting clamping block and the supporting block.
Further, the last fixed surface of shunt is connected with the connecting pipe, the shunt passes through connecting pipe and inner shell swing joint, the lower fixed surface of shunt is connected with divides the stream block, the surface of dividing the stream block is provided with the first reposition of redundant personnel hole of a plurality of, the surface of dividing the stream block is provided with the second reposition of redundant personnel hole near the position in the middle.
Furthermore, a fixed bottom block and a connecting plate are fixedly mounted inside the supporting block, the fixed bottom block is located below the connecting plate, and a vibrator is arranged on the inner surface of the supporting block.
Further, the last fixed surface of fixed bottom piece is connected with spring and first elasticity clamp splice, the spring is located one side of first elasticity clamp splice, the lower fixed surface of connecting plate is connected with second elasticity clamp splice, fixedly connected with elastic support post between first elasticity clamp splice and the second elasticity clamp splice, the last fixed surface of connecting plate installs the connecting block, the last fixed surface of connecting block installs fixed clamp splice, the connecting plate passes through spring and elastic support post and fixed bottom piece fixed connection.
Furthermore, the inner surface of the first clamping ring is provided with a fixing lug, and a fixing ring is movably arranged between the first clamping ring and the first dialysis membrane.
Further, first dialysis membrane passes through solid fixed ring and first clamp ring fixed connection, first clamp ring passes through fixed lug and dialysis main part fixed connection, the position between fixed lug and the solid fixed ring is provided with sealed the pad.
The protein dialysis device comprises the following working steps:
step one, inputting collected protein dialysate into an inner shell through a material guide pipe, and shunting the protein dialysate through a first shunting hole and a second shunting hole on a shunting block when the protein dialysate passes through a shunt so that the shunted protein dialysate is uniformly sprinkled on a first dialysis membrane;
step two, starting a vibrator, enabling the fixed bottom block to vibrate, enabling the fixed bottom block to drive a connecting plate to vibrate left and right through a spring and an elastic supporting column, further enabling a dialysis main body to vibrate, enabling protein dialysate to generate first dialysate after being dialyzed by a first dialysis membrane under the action of gravity and shaking of the dialysis main body, enabling the first dialysate to enter a second dialysis membrane, and enabling the first dialysate to continuously penetrate through the second dialysis membrane under the action of gravity and shaking of the dialysis main body;
and step three, the first dialysate is dialyzed and filtered by the second dialysis membrane to generate second dialysate, and the second dialysate is stored at the bottom of the dialysis main body, after the first dialysate is dialyzed, the vibrator is stopped to work, and different dialyzates generated by the separation of the dialysis main body are collected through the first liquid guide port, the second liquid guide port and the third liquid guide port.
The invention has the beneficial effects that:
(1) according to the invention, through the matched use of the fixed bottom block, the spring and the vibrator, the dialysis main body on the fixed bottom block is shaken through the left-right vibration of the vibrator, so that dialysate can better permeate a dialysis membrane, the efficiency and quality of dialysis are effectively improved, and the problems of position deviation and toppling of the dialysis main body in the shaking process can be avoided due to the matched use of the fixed column, the spring and the elastic support column;
(2) the invention can uniformly spray the introduced liquid on the dialysis membrane through the shunt by the shunt, can effectively increase the dialysis speed and the dialysis quality by shunting the dialysate, and can avoid the damage of the dialysis membrane caused by impact;
(3) according to the invention, the first dialysis membrane, the second dialysis membrane, the fixing lug and the fixing ring are matched for use, so that the dialysis quality can be effectively improved when dialysate is dialyzed by the two layers of dialysis membranes, and the layering effect of the dialysate is better under the action of gravity and shaking force from top to bottom.
Drawings
In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic structural diagram of a protein dialysis apparatus according to the present invention;
FIG. 2 is a rear view of a protein dialysis device according to the present invention;
FIG. 3 is a schematic view of a connection structure of a fixed bottom block and a connecting plate according to the present invention;
FIG. 4 is a schematic view of the diverter of the present invention;
FIG. 5 is a schematic view of the structure of a first dialysis membrane according to the present invention.
In the figure: 1. a dialysis subject; 2. a material guide pipe; 3. an inner shell; 4. a flow divider; 5. a first dialysis membrane; 6. a second dialysis membrane; 7. fixing the bottom block; 8. a first liquid guide port; 9. a second liquid guide port; 10. a third liquid guide port; 11. a support block; 12. fixing a column; 13. a first clamp ring; 14. a second clamp ring; 15. supporting the clamping block; 16. a connecting plate; 17. a spring; 18. a first resilient clamping block; 19. an elastomeric support column; 20. a second elastic clamping block; 21. connecting blocks; 22. fixing the clamping block; 23. a connecting pipe; 24. a shunting block; 25. a first diverter orifice; 26. a second flow dividing orifice; 27. fixing the bump; 28. and (4) fixing the ring.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 5, a protein dialysis apparatus comprises a dialysis main body 1, wherein a material guide tube 2 is embedded in a position close to the upper part inside the dialysis main body 1, an inner shell 3 is fixedly installed in a position close to the middle inside the dialysis main body 1, a flow divider 4 is movably connected to a position close to the upper part on the inner surface of the inner shell 3, a first dialysis membrane 5 and a second dialysis membrane 6 are fixedly installed inside the inner shell 3, the first dialysis membrane 5 is located above the second dialysis membrane 6, a support block 11 is fixedly installed on the lower surface of the dialysis main body 1, a first liquid guide port 8 and a first clamp ring 13 are embedded in a position close to the upper part on the outer surface of the dialysis main body 1, the first liquid guide port 8 is located above the first clamp ring 13, a second liquid guide port 9 and a second clamp ring 14 are embedded in a position close to the middle on the outer surface of the dialysis main body 1, the second liquid guide port 9 is positioned above the second clamping ring 14, and a third liquid guide port 10 is embedded in a position, close to the lower part, of the outer surface of the dialysis main body 1.
One side of the dialysis main body 1 is fixedly provided with a fixing column 12, one side of the fixing column 12 close to the dialysis main body 1 is fixedly provided with a supporting clamping block 15, and the fixing column 12 is movably connected with the dialysis main body 1 through the supporting clamping block 15 and the supporting block 11.
The last fixed surface of shunt 4 is connected with connecting pipe 23, shunt 4 passes through connecting pipe 23 and inner shell 3 swing joint, the lower fixed surface of shunt 4 is connected with divides stream block 24, the surface of dividing stream block 24 is provided with the first reposition of redundant personnel hole 25 of a plurality of, the surface of dividing stream block 24 is provided with the second reposition of redundant personnel hole 26 near the position in the middle.
The inner part of the supporting block 11 is fixedly provided with a fixed bottom block 7 and a connecting plate 16, the fixed bottom block 7 is positioned below the connecting plate 16, and the inner surface of the supporting block 11 is provided with a vibrator.
Fixed surface 7's last fixed surface is connected with spring 17 and first elasticity clamp splice 18, spring 17 is located one side of first elasticity clamp splice 18, the lower fixed surface of connecting plate 16 is connected with second elasticity clamp splice 20, fixedly connected with elastic support post 19 between first elasticity clamp splice 18 and the second elasticity clamp splice 20, the last fixed surface of connecting plate 16 installs connecting block 21, the last fixed surface of connecting block 21 installs fixed clamp splice 22, connecting plate 16 passes through spring 17 and elastic support post 19 and fixed bottom 7 fixed connection.
The inner surface of the first clamping ring 13 is provided with a fixing lug 27, and a fixing ring 28 is movably arranged between the first clamping ring 13 and the first dialysis membrane 5.
First dialysis membrane 5 is through solid fixed ring 28 and first clamp ring 13 fixed connection, first clamp ring 13 is through fixed lug 27 and dialysis main part 1 fixed connection, the position between fixed lug 27 and the solid fixed ring 28 is provided with sealed the pad.
The protein dialysis device comprises the following working steps:
firstly, inputting collected protein dialysate into an inner shell 3 through a material guide pipe 2, and when the protein dialysate passes through a flow divider 4, dividing the protein dialysate through a first flow dividing hole 25 and a second flow dividing hole 26 on a flow dividing block 24 so that the divided protein dialysate is uniformly sprinkled on a first dialysis membrane 5;
step two, starting a vibrator, enabling the fixed bottom block 7 to vibrate, enabling the fixed bottom block 7 to drive the connecting plate 16 to vibrate left and right through the spring 17 and the elastic supporting column 19, further enabling the dialysis main body 1 to vibrate, enabling protein dialysate to generate first dialysate through the first dialysis membrane 5 under the action of gravity and shaking of the dialysis main body 1, enabling the first dialysate to enter the second dialysis membrane 6, and enabling the first dialysate to continuously penetrate through the second dialysis membrane 6 under the action of gravity and shaking of the dialysis main body 1;
and step three, the first dialysate is dialyzed and filtered by the second dialysis membrane 6 to generate second dialysate, and the second dialysate is stored at the bottom of the dialysis main body 1, after the first dialysate is dialyzed, the vibrator is stopped to work, and different dialyzates generated by the separation of the dialysis main body 1 are collected through the first liquid guide port 8, the second liquid guide port 9 and the third liquid guide port 10.
A protein dialysis device, when in use, the invention inputs the collected protein dialysate into an inner shell 3 through a material guide pipe 2, when the protein dialysate passes through a shunt 4, the upper surface of the shunt 4 is fixedly connected with a connecting pipe 23, the shunt 4 is movably connected with the inner shell 3 through the connecting pipe 23, the lower surface of the shunt 4 is fixedly connected with a shunt block 24, the outer surface of the shunt block 24 is provided with a plurality of first shunt holes 25, the outer surface of the shunt block 24 is provided with a second shunt hole 26 near the middle, shunt is carried out through the first shunt hole 25 and the second shunt hole 26 on the shunt block 24, so that the shunted protein dialysate is uniformly sprinkled on a first dialysis membrane 5, the first dialysis membrane 5 is fixedly connected with a first clamping ring 13 through a fixing ring 28, the first clamping ring 13 is fixedly connected with a dialysis main body 1 through a fixing lug 27, the inner surface of the first clamping ring 13 is provided with a fixing lug 27, a fixing ring 28 is movably arranged between the first clamping ring 13 and the first dialysis membrane 5, and a sealing gasket is arranged between the fixing bump 27 and the fixing ring 28 and plays a role in sealing and isolating; a fixed bottom block 7 and a connecting plate 16 are fixedly arranged inside the supporting block 11, the fixed bottom block 7 is positioned below the connecting plate 16, a vibrator of MVE series vibration motor type is arranged on the inner surface of the supporting block 11 and is started to enable the fixed bottom block 7 to vibrate, the fixed bottom block 7 drives the connecting plate 16 to vibrate through a spring 17 and an elastic supporting column 19, the upper surface of the fixed bottom block 7 is fixedly connected with the spring 17 and a first elastic clamping block 18, the spring 17 is positioned on one side of the first elastic clamping block 18, the lower surface of the connecting plate 16 is fixedly connected with a second elastic clamping block 20, the elastic supporting column 19 is fixedly connected between the first elastic clamping block 18 and the second elastic clamping block 20, a connecting block 21 is fixedly arranged on the upper surface of the connecting plate 16, a fixed clamping block 22 is fixedly arranged on the upper surface of the connecting block 21, and the connecting plate 16 is fixedly connected with the fixed bottom block 7 through the spring 17 and the elastic, further vibrating the dialysis main body 1, dialyzing the protein dialysate by the first dialysis membrane 5 under the action of gravity and shaking of the dialysis main body 1 to generate a first dialysate, and allowing the first dialysate to enter the second dialysis membrane 6, wherein the first dialysate continuously penetrates through the second dialysis membrane 6 under the action of gravity and shaking of the dialysis main body 1; the first dialysate is dialyzed and filtered by the second dialysis membrane 6 to generate second dialysate, and is stored at the bottom of the dialysis main body 1, after the first dialysate is dialyzed, the vibrator stops working, and different dialyzates generated by the dialysis main body 1 are separated through the first liquid guide port 8, the second liquid guide port 9 and the third liquid guide port 10 and are collected;
through the shunt 4 that sets up, can pass through the even spilling of shunt 4 with leading-in liquid on the dialysis membrane, can effectively increase the speed and the dialysis quality of dialysis, solved in the past equipment and not shunted dialysis liquid and then lead to the dialysis membrane to receive the impact and cause the damage and the problem that the dialysis efficiency is low.
Through the fixed bottom block 7 that sets up, the cooperation of spring 17 and vibrator is used, left and right vibration through the vibrator, and then make dialysis main part 1 on the fixed bottom block 7 rock, can let better the penetrating dialysis membrane of dislysate, the efficiency and the quality of dialysis have effectively been improved, fixed column 12, spring 17 and elastic support post 19's cooperation is used, can make dialysis main part 1 can not take place the problem that the skew and topple over at the in-process of rocking, the mode through the stirring among the prior art has been solved and the rate of dialysis is improved, but the problem of destroying the dislysate cell easily exists in the mode of stirring.
The cooperation through the first dialysis membrane 5 that sets up, second dialysis membrane 6, fixed lug 27 and solid fixed ring 28 is used for the quality of dialysis can effectively be improved under the dialysis of dialysate process two-layer dialysis membrane, and the dialysate down by gravity and the effect of shaking down from last in addition, makes the effect of dialysate layering better, has solved the not good problem of dialysis quality that leads to of dialysate separation back liquid reflux in the past.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims (8)

1. The protein dialysis device is characterized by comprising a dialysis main body (1), wherein a material guide pipe (2) is embedded in a position, close to the upper part, in the dialysis main body (1), an inner shell (3) is fixedly installed in a position, close to the middle part, in the dialysis main body (1), a shunt (4) is movably connected to a position, close to the upper part, of the inner surface of the inner shell (3), a first dialysis membrane (5) and a second dialysis membrane (6) are fixedly installed in the inner part of the inner shell (3), the first dialysis membrane (5) is located above the second dialysis membrane (6), a supporting block (11) is fixedly installed on the lower surface of the dialysis main body (1), a first liquid guide port (8) and a first clamping ring (13) are embedded in a position, close to the upper part, of the outer surface of the dialysis main body (1), the first liquid guide port (8) is located above the first clamping ring (13), the surface of dialysis main part (1) is inlayed near the position in middle and is had second drain mouth (9) and second clamp ring (14), second drain mouth (9) are located the top of second clamp ring (14), the surface of dialysis main part (1) is inlayed near the position of below and is had third drain mouth (10).
2. The protein dialysis device as claimed in claim 1, wherein a fixing column (12) is fixedly mounted on one side of the dialysis main body (1), a supporting clamp block (15) is fixedly mounted on one side of the fixing column (12) close to the dialysis main body (1), and the fixing column (12) is movably connected with the dialysis main body (1) through the supporting clamp block (15) and the supporting block (11).
3. The protein dialysis device according to claim 1, wherein the upper surface of the shunt (4) is fixedly connected with a connecting pipe (23), the shunt (4) is movably connected with the inner shell (3) through the connecting pipe (23), the lower surface of the shunt (4) is fixedly connected with a shunt block (24), the outer surface of the shunt block (24) is provided with a plurality of first shunt holes (25), and the outer surface of the shunt block (24) is provided with a second shunt hole (26) near the middle.
4. A protein dialysis device according to claim 3, wherein the supporting block (11) is internally and fixedly provided with a fixed bottom block (7) and a connecting plate (16), the fixed bottom block (7) is positioned below the connecting plate (16), and the inner surface of the supporting block (11) is provided with a vibrator.
5. The protein dialysis device according to claim 4, wherein a spring (17) and a first elastic clamping block (18) are fixedly connected to the upper surface of the fixed bottom block (7), the spring (17) is located on one side of the first elastic clamping block (18), a second elastic clamping block (20) is fixedly connected to the lower surface of the connecting plate (16), an elastic supporting column (19) is fixedly connected between the first elastic clamping block (18) and the second elastic clamping block (20), a connecting block (21) is fixedly installed on the upper surface of the connecting plate (16), a fixed clamping block (22) is fixedly installed on the upper surface of the connecting block (21), and the connecting plate (16) is fixedly connected with the fixed bottom block (7) through the spring (17) and the elastic supporting column (19).
6. The protein dialysis device according to claim 1, wherein the inner surface of the first clamping ring (13) is provided with a fixing protrusion (27), and a fixing ring (28) is movably mounted between the first clamping ring (13) and the first dialysis membrane (5).
7. The protein dialysis device according to claim 6, wherein the first dialysis membrane (5) is fixedly connected with the first clamping ring (13) through a fixing ring (28), the first clamping ring (13) is fixedly connected with the dialysis body (1) through a fixing bump (27), and a sealing gasket is arranged between the fixing bump (27) and the fixing ring (28).
8. A protein dialysis device and a dialysis method are characterized in that the working steps of the device are as follows:
step one, inputting collected protein dialysate into an inner shell (3) through a material guide pipe (2), and when the protein dialysate passes through a flow divider (4), dividing the protein dialysate through a first flow dividing hole (25) and a second flow dividing hole (26) on a flow dividing block (24), so that the divided protein dialysate is uniformly sprinkled on a first dialysis membrane (5);
step two, starting a vibrator, enabling the fixed bottom block (7) to vibrate, enabling the fixed bottom block (7) to drive the connecting plate (16) to vibrate left and right through the spring (17) and the elastic supporting column (19), further enabling the dialysis main body (1) to vibrate, enabling protein dialysate to generate first dialysate after being dialyzed by the first dialysis membrane (5) under the action of gravity and shaking of the dialysis main body (1), enabling the first dialysate to enter the second dialysis membrane (6), and enabling the first dialysate to continuously penetrate through the second dialysis membrane (6) under the action of gravity and shaking of the dialysis main body (1);
and step three, the first dialysate is dialyzed and filtered by the second dialysis membrane (6) to generate second dialysate, and is stored at the bottom of the dialysis main body (1), after the first dialysate is dialyzed, the vibrator is stopped to work, and different dialyzates generated by the separation of the dialysis main body (1) are collected through the first liquid guide port (8), the second liquid guide port (9) and the third liquid guide port (10).
CN202010163179.8A 2020-03-10 2020-03-10 Protein dialysis device and dialysis method Withdrawn CN111349143A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010163179.8A CN111349143A (en) 2020-03-10 2020-03-10 Protein dialysis device and dialysis method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010163179.8A CN111349143A (en) 2020-03-10 2020-03-10 Protein dialysis device and dialysis method

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115611974A (en) * 2022-10-24 2023-01-17 上海华新生物高技术有限公司 Filtering and sterilizing equipment for preparing interferon purified protein

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115611974A (en) * 2022-10-24 2023-01-17 上海华新生物高技术有限公司 Filtering and sterilizing equipment for preparing interferon purified protein
CN115611974B (en) * 2022-10-24 2023-06-06 上海华新生物高技术有限公司 Filter sterilization equipment for preparing interferon purified protein

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Application publication date: 20200630