CN115611974B

CN115611974B - Filter sterilization equipment for preparing interferon purified protein

Info

Publication number: CN115611974B
Application number: CN202211302355.7A
Authority: CN
Inventors: 张顺荣; 蔡绘图; 陆凯磊; 刘惠
Original assignee: SHANGHAI HUAXIN HIGH BIOTECHNOLOGY Inc
Current assignee: SHANGHAI HUAXIN HIGH BIOTECHNOLOGY Inc
Priority date: 2022-10-24
Filing date: 2022-10-24
Publication date: 2023-06-06
Anticipated expiration: 2042-10-24
Also published as: CN115611974A

Abstract

The invention discloses filter sterilization equipment for preparing purified protein of interferon, which relates to the technical field of interferon processing and comprises a filter sterilization frame, wherein a liquid inlet hopper is fixedly arranged at the top of the filter sterilization frame, a filter chamber is arranged below the liquid inlet hopper, the bottom of the liquid inlet hopper is communicated with the top of the filter chamber, a drainage tube is fixedly arranged at the bottom of the liquid inlet hopper, a first drainage plate and a second drainage plate are fixedly arranged in the filter chamber, a lifting hopper is slidably arranged between the first drainage plate and the second drainage plate, a dialysis membrane is fixedly arranged on the lifting hopper, a guide roller is rotatably arranged in the filter chamber, and the guide roller is matched with the lifting hopper which is repeatedly lifted in the filter chamber, so that impurities on the surface of the dialysis membrane can be efficiently removed by the guide roller when an interferon solution is subjected to vibration filtration, the condition that the surface of the dialysis membrane is blocked is avoided, and the filtration efficiency of the dialysis membrane is further improved.

Description

Filter sterilization equipment for preparing interferon purified protein

Technical Field

The invention relates to the technical field of interferon processing, in particular to filter sterilization equipment for preparing an interferon purified protein.

Background

The interferon is a glycoprotein with high species specificity, so that the interferon of animals is ineffective for human beings, and has the functions of resisting viruses, inhibiting cell proliferation, regulating immunity and resisting tumors.

The interferon is prepared by using a filtration sterilization device, and the current filtration sterilization device filters an interferon solution through a dialysis membrane and performs sterilization treatment through a sterilizing lamp. The dialysis membrane in the existing filtration and sterilization equipment is often easy to be blocked, and the conventional vibration structure cannot effectively remove impurities on the surface of the dialysis membrane, so that the dialysis membrane needs to be manually replaced or cleaned periodically. And the sterilizing lamp in the filtering sterilizing equipment can carry out effective sterilizing treatment on all interferon solutions after being irradiated for a period of time, and the sterilizing efficiency is not high.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a filter sterilization device for preparing interferon purified protein.

In order to achieve the above purpose, the present invention provides the following technical solutions:

the utility model provides a be used for filter sterilization equipment for interferon purification protein preparation, includes filter sterilization frame, filter sterilization frame top fixed mounting has the feed liquor fill, be provided with the filter chamber below the feed liquor fill, feed liquor fill bottom intercommunication filter chamber top, feed liquor fill bottom fixed mounting has the drainage tube, fixed mounting has first drainage board, second drainage board in the filter chamber, slidable mounting has the lift fight between first drainage board and the second drainage board, fixed mounting has the dialysis membrane on the lift fight, guide roller is installed to the filter chamber internal rotation, filter chamber outer wall fixed mounting has the wash pump, wash pump one side fixed mounting has the wash water tank, wash pump water pumping end intercommunication wash water tank lateral wall, wash pump water outlet end rotates and connects the adapter tube, adapter tube keeps away from wash pump one end fixed connection guide roller, a plurality of wash holes have been seted up on the guide roller surface, the guide roller sets up directly over the lift fight, lift fight bottom fixed mounting has the flexible pipe;

the sterilization device comprises a filtering chamber, and is characterized in that a sterilization chamber is arranged below the filtering chamber, a shunt box is fixedly arranged at the bottom of the telescopic pipe, a plurality of first sterilizing lamps are arranged below the shunt box, a winding pipe is wound on the first sterilizing lamps, the shunt box is fixedly connected with the winding pipe, the winding pipe is far away from an annular pipe fixedly connected with one end of the shunt box, a plurality of connecting pipes are fixedly arranged on the annular pipe, a liquid outlet pipe is fixedly connected with the plurality of connecting pipes, a rotary toothed ring is rotatably arranged in the sterilization chamber, and a plurality of second sterilizing lamps are fixedly arranged on the rotary toothed ring.

Further, the first drainage plate and the second drainage plate are all obliquely arranged, a lifting assembly is fixedly arranged at the bottom of the inner wall of the filtering chamber, and the lifting assembly is used for driving the lifting bucket to lift.

Further, the outer wall of the filtering chamber is fixedly provided with a driving assembly, and the driving assembly is used for driving the guide roller to rotate.

Further, a discharge hole is formed in one side of the filtering chamber, a drain pipe is fixedly mounted on the filtering sterilization frame, and the drain pipe is communicated with the discharge hole.

Further, a plurality of first sterilizing lamps are equally spaced annular distribution, and a plurality of connecting pipes are fixedly arranged on the annular pipes in an equal radian mode.

Further, one end of the liquid outlet pipe far away from the connecting pipe penetrates through the side wall of the sterilization chamber.

Further, radian fixed mounting such as a plurality of second sterilizing lamps is on rotatory ring gear, the indoor chamber bottom fixed mounting of degerming has rotating assembly, rotating assembly is used for driving rotatory gear rotation, rotatory ring gear is connected in rotatory gear meshing.

Compared with the prior art, the invention has the following beneficial effects:

1. the rotary guide roller is arranged in the filter chamber and is matched with the lifting hopper which is repeatedly lifted, so that the guide roller can effectively remove impurities on the surface of the dialysis membrane while vibrating and filtering the interferon solution, the blocking condition of the surface of the dialysis membrane is avoided, the filter efficiency of the dialysis membrane is further improved, and after the filtration and sterilization are carried out, the guide roller can spray water to correspondingly clean the dialysis membrane, the replacement and the cleaning times of the dialysis membrane are reduced, and the guide roller can play a role in improving the filter efficiency of the dialysis membrane and can correspondingly clean the dialysis membrane;

2. the sterilizing device comprises a sterilizing chamber, a plurality of first sterilizing lamps, a winding tube and a plurality of second sterilizing lamps, wherein the first sterilizing lamps are arranged in the sterilizing chamber, the winding tube is arranged on the first sterilizing lamps, the second sterilizing lamps are arranged in the sterilizing chamber and are used for shunting an interferon solution, the flow of the interferon solution is reduced, the sterilizing effect and the sterilizing efficiency of the first sterilizing lamps and the second sterilizing lamps on the interferon solution are improved, the interferon solution can be sterilized in the flowing process, and the first sterilizing lamps and the second sterilizing lamps are used for guaranteeing the sterilizing treatment of the interferon solution in the winding tube without dead angles.

Drawings

FIG. 1 is a schematic diagram of a filter sterilization apparatus for the preparation of interferon purified proteins;

figure 2 is an internal structural view of the filtering chamber of the present invention;

FIG. 3 is an internal structural view of the guide roller of the present invention;

FIG. 4 is an internal structural view of the lift bucket of the present invention;

figure 5 is a side view of the filter chamber of the present invention;

FIG. 6 is an internal construction view of the sterilization chamber of the present invention;

FIG. 7 is an installation view of a first germicidal lamp in accordance with the present invention;

fig. 8 is a schematic structural view of the annular tube of the present invention.

100. A filter sterilization rack; 101. a liquid inlet hopper; 102. a filtering chamber; 103. a drive assembly; 104. a guide roller; 1041. a connecting pipe; 1042. cleaning the hole; 105. a drainage tube; 106. a first drainage plate; 107. a second drainage plate; 108. lifting bucket; 1081. a dialysis membrane; 109. a lifting assembly; 110. a telescopic tube; 111. a cleaning pump; 112. cleaning the water tank; 113. a discharge hole; 114. a blow-down pipe; 200. a sterilization chamber; 201. a shunt box; 202. winding a tube; 203. a first sterilizing lamp; 204. an annular tube; 205. a connecting pipe; 206. a liquid outlet pipe; 207. a rotating assembly; 208. a rotary gear; 209. rotating the toothed ring; 210. and a second sterilizing lamp.

Detailed Description

Example 1

Referring to fig. 1 to 5, a filter sterilization device for preparing purified protein of interferon comprises a filter sterilization frame 100, wherein a liquid inlet hopper 101 is fixedly installed at the top of the filter sterilization frame 100, a filter chamber 102 is arranged below the liquid inlet hopper 101, the bottom of the liquid inlet hopper 101 is communicated with the top of the filter chamber 102, a drainage tube 105 is fixedly installed at the bottom of the liquid inlet hopper 101, a first drainage plate 106 and a second drainage plate 107 are fixedly installed in the filter chamber 102, a lifting hopper 108 is slidably installed between the first drainage plate 106 and the second drainage plate 107, a dialysis membrane 1081 is fixedly installed on the lifting hopper 108, a guide roller 104 is rotatably installed in the filter chamber 102, a cleaning pump 111 is fixedly installed on the outer wall of the filter chamber 102, a cleaning water tank 112 is fixedly installed on one side of the cleaning pump 111, a water outlet end of the cleaning pump 111 is rotatably connected with a connecting tube 1041, one end of the connecting tube 1041, which is far away from the cleaning pump 111, a guide roller 104 is fixedly connected with a plurality of cleaning holes 1042 are formed in the surface of the guide roller 104, the guide roller 104 is arranged right above the lifting hopper 108, and a telescopic tube 110 is fixedly installed at the bottom of the lifting hopper 108. The first drainage plate 106 and the second drainage plate 107 are all inclined, and a lifting assembly 109 is fixedly arranged at the bottom of the inner wall of the filtering chamber 102, and the lifting assembly 109 can be an air cylinder or an oil cylinder, and is used for driving a lifting bucket 108 to lift. The outer wall of the filtering chamber 102 is fixedly provided with a driving component 103, the driving component 103 can be a motor, and the driving component 103 is used for driving the guide roller 104 to rotate. A discharge hole 113 is formed in one side of the filtering chamber 102, a drain pipe 114 is fixedly arranged on the filtering and sterilizing frame 100, and the drain pipe 114 is communicated with the discharge hole 113. Set up rotatory guide roll 104 in filter chamber 102, the lift fill 108 that cooperates repeatedly goes up and down, guide roll 104 can be with the impurity high-efficient removal of dialysis membrane 1081 surface when vibration filtration for the interferon solution, avoid the circumstances that the dialysis membrane 1081 surface appears blocking, and then improve the filtration efficiency of dialysis membrane 1081, and after filtration degerming, guide roll 104 can spray water and carry out corresponding washing to dialysis membrane 1081, reduce the change and the washing number of times of dialysis membrane 1081, guide roll 104 not only can play the effect that improves dialysis membrane 1081 filtration efficiency, can carry out corresponding washing to dialysis membrane 1081 simultaneously.

Example 2

Referring to fig. 6 to 8, based on embodiment 1, a sterilization chamber 200 is provided below a filter chamber 102, a shunt box 201 is fixedly installed at the bottom of a telescopic tube 110, a plurality of first sterilizing lamps 203 are provided below the shunt box 201, a winding tube 202 is wound on the first sterilizing lamps 203, the winding tube 202 is fixedly connected with the shunt box 201, one end of the winding tube 202, far away from the shunt box 201, is fixedly connected with an annular tube 204, a plurality of connecting tubes 205 are fixedly installed on the annular tube 204, a plurality of connecting tubes 205 are fixedly connected with a liquid outlet tube 206, a rotary toothed ring 209 is rotatably installed in the sterilization chamber 200, and a plurality of second sterilizing lamps 210 are fixedly installed on the rotary toothed ring 209. The first sterilizing lamps 203 are distributed in an equidistant annular shape, and the connecting pipes 205 are fixedly arranged on the annular pipe 204 in an equal radian. The outlet pipe 206 penetrates the side wall of the sterilization chamber 200 at the end far away from the connection pipe 205. The radian fixed mounting such as a plurality of second sterilizing lamps 210 is on rotatory ring gear 209, and sterilization room 200 inner chamber bottom fixed mounting has rotary component 207, and rotary component 207 can be motor, gyration cylinder, and rotary component 207 is used for driving rotation gear 208 rotatory, and rotation gear 208 meshing is connected rotatory ring gear 209. A plurality of first sterilizing lamps 203 are arranged in the sterilizing chamber 200, a winding tube 202 is arranged on the first sterilizing lamps 203, a plurality of second sterilizing lamps 210 which rotate circularly are arranged in the sterilizing chamber 200, the plurality of winding tubes 202 shunt the interferon solution, the flow of the interferon solution is reduced, the sterilizing effect of the first sterilizing lamps 203 and the second sterilizing lamps 210 on the interferon solution is improved, and the first sterilizing lamps 203 and the second sterilizing lamps 210 ensure that the interferon solution in the winding tubes 202 is sterilized without dead angles.

Working principle:

step one: adding an interferon solution into the liquid inlet bucket 101, enabling the interferon solution to flow to the first drainage plate 106 through the drainage tube 105, enabling the first drainage plate 106 to guide the interferon solution to the lifting bucket 108, repeatedly pushing and pulling the lifting bucket 108 through the lifting assembly 109, repeatedly lifting the lifting bucket 108 between the first drainage plate 106 and the second drainage plate 107, simultaneously driving the guide roller 104 to rotate through the driving assembly 103, enabling the interferon solution to pass through the dialysis membrane 1081 and flow into the telescopic tube 110 in the lifting process of the lifting bucket 108, enabling impurities to be adhered to the surface of the dialysis membrane 1081, enabling the rotating guide roller 104 to drive the impurities on the surface of the dialysis membrane 1081 to move towards the second drainage plate 107 in the lifting process of the lifting bucket 108 when the surface of the guide roller 104 is contacted with the surface of the dialysis membrane 1081, enabling the impurities to fall on the second drainage plate 107 and flow to the drain pipe 114 through the second drainage plate 107;

step two: the interferon solution passes through the telescopic tube 110 and flows into the shunt box 201, the interferon solution in the shunt box 201 flows into the winding tube 202, meanwhile, the rotating assembly 207 drives the rotating gear 208 to rotate, the rotating gear 208 drives the rotating gear ring 209 to rotate, the rotating gear ring 209 drives the plurality of second sterilizing lamps 210 to rotate, the first sterilizing lamps 203 cooperate with the second sterilizing lamps 210 to sterilize the interferon solution in the winding tube 202, the sterilized interferon solution flows into the connecting tube 205, and the interferon solution flows into the liquid outlet tube 206 through the connecting tube 205;

step three: after the interferon solution is filtered and sterilized, the cleaning pump 111 pumps water in the cleaning water tank 112, and conveys the water into the guide roller 104 through the connecting pipe 1041, the water in the guide roller 104 is discharged through the cleaning hole 1042, meanwhile, the driving component 103 drives the guide roller 104 to rotate, the lifting component 109 drives the lifting bucket 108 to lift repeatedly, when the surface of the guide roller 104 contacts with the surface of the dialysis membrane 1081 in the lifting process of the lifting bucket 108, the rotating guide roller 104 cooperates with the water discharged from the cleaning hole 1042 to clean the impurities remained on the surface of the dialysis membrane 1081 onto the second drainage plate 107, part of the water flows into the telescopic pipe 110 through the dialysis membrane 1081, and the follow-up diversion box 201, the winding pipe 202, the annular pipe 204, the connecting pipe 205 and the liquid outlet pipe 206 are cleaned in sequence.

The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above examples, and all technical solutions belonging to the concept of the present invention belong to the protection scope of the present invention. It should be noted that modifications and adaptations to those skilled in the art without departing from the principles of the present invention are intended to be considered as protecting the scope of the present template.

Claims

1. The utility model provides a filter sterilization equipment for interferon purification protein preparation, includes filter sterilization frame (100), its characterized in that, filter sterilization frame (100) top fixed mounting has feed liquor fill (101), feed liquor fill (101) below is provided with filter chamber (102), feed liquor fill (101) bottom intercommunication filter chamber (102) top, feed liquor fill (101) bottom fixed mounting has drainage tube (105), filter chamber (102) internally fixed mounting has first drainage plate (106), second drainage plate (107), slidable mounting has lift fill (108) between first drainage plate (106) and second drainage plate (107), fixed mounting has dialysis membrane (1081) on lift fill (108), guide roller (104) are installed in filter chamber (102) internal rotation, filter chamber (102) outer wall fixed mounting has wash pump (111), wash pump (111) one side fixed mounting has wash water tank (112), wash pump (111) end intercommunication wash water tank (112) lateral wall, wash pump (111) go out and connect (1041) and take in guide roller (1041) and keep away from guide roller (1041) and offer a plurality of guide roller (1041) and connect (1041) surface to connect and take up a plurality of guide roller (104), the guide roller (104) is arranged right above the lifting bucket (108), and a telescopic pipe (110) is fixedly arranged at the bottom of the lifting bucket (108);

the sterilization device comprises a filtering chamber (102), a sterilization chamber (200) is arranged below the filtering chamber (102), a shunt box (201) is fixedly arranged at the bottom of a telescopic pipe (110), a plurality of first sterilization lamps (203) are arranged below the shunt box (201), a winding pipe (202) is wound on the first sterilization lamps (203), the winding pipe (202) is fixedly connected with the shunt box (201), the winding pipe (202) is far away from an annular pipe (204) fixedly connected with one end of the shunt box (201), a plurality of connecting pipes (205) are fixedly arranged on the annular pipe (204), a plurality of connecting pipes (205) are fixedly connected with a liquid outlet pipe (206), a rotary toothed ring (209) is rotationally arranged in the sterilization chamber (200), and a plurality of second sterilization lamps (210) are fixedly arranged on the rotary toothed ring (209).

2. The filter sterilization device for preparing the interferon purified protein according to claim 1, wherein the first drainage plate (106) and the second drainage plate (107) are obliquely arranged, a lifting assembly (109) is fixedly arranged at the bottom of the inner wall of the filtering chamber (102), and the lifting assembly (109) is used for driving the lifting bucket (108) to lift.

3. The filter sterilization device for preparing interferon purified protein according to claim 2, wherein a driving component (103) is fixedly arranged on the outer wall of the filtering chamber (102), and the driving component (103) is used for driving the guide roller (104) to rotate.

4. A filter sterilization device for preparing an interferon purified protein according to claim 3, wherein a discharge hole (113) is formed on one side of the filter chamber (102), a drain pipe (114) is fixedly installed on the filter sterilization frame (100), and the drain pipe (114) is communicated with the discharge hole (113).

5. The filter sterilization device for preparing an interferon purified protein according to claim 4, wherein the plurality of first sterilization lamps (203) are distributed in an equidistant annular shape, and the plurality of connecting pipes (205) are fixedly arranged on the annular pipe (204) in an equal radian.

6. The filter sterilization apparatus for interferon purified protein production according to claim 1, wherein the outlet pipe (206) penetrates the side wall of the sterilization chamber (200) at an end far away from the connection pipe (205).

7. The filter sterilization device for preparing the interferon purified protein according to claim 6, wherein a plurality of second sterilizing lamps (210) are fixedly arranged on a rotary toothed ring (209) in an equal radian, a rotary assembly (207) is fixedly arranged at the bottom of an inner cavity of the sterilization chamber (200), the rotary assembly (207) is used for driving a rotary gear (208) to rotate, and the rotary gear (208) is in meshed connection with the rotary toothed ring (209).