CN111304063A

CN111304063A - Tumor cell culture bottle preprocessing device

Info

Publication number: CN111304063A
Application number: CN202010094531.7A
Authority: CN
Inventors: 秦保珍
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-02-16
Filing date: 2020-02-16
Publication date: 2020-06-19

Abstract

The invention relates to a tumor cell culture device, in particular to a tumor cell culture bottle pretreatment device. The invention aims to provide a pretreatment device for a tumor cell culture bottle. A tumor cell culture bottle pretreatment device comprises a right bottom frame, a real-time control screen, a left bottom frame, an inner support plate, a sterilization ventilation net, a top curved plate, a collagen thin layer covering mechanism, a sterile sealing adding mechanism and a dead-angle-free rotary culture mechanism; a real-time control screen is arranged in the middle of the right end of the right bottom frame; the bottom of the left end of the right bottom frame is connected with the left bottom frame; the left side of the bottom end in the right bottom frame is connected with the inner supporting plate. The invention realizes the uniform and all-round coverage of the collagen thin layer of the tumor cell culture bottle, the aseptic addition of the culture solution and the regular shaking of the culture bottle in the culture process, namely the effect of the culture bottle pretreatment device with the uniform distribution of nutrient substances.

Description

Tumor cell culture bottle preprocessing device

Technical Field

The invention relates to a tumor cell culture device, in particular to a tumor cell culture bottle pretreatment device.

Background

Tumors become one of the most difficult diseases for human beings at present, the special function of early discovering cell canceration is provided for culturing tumor cell growth, the tumor cell growth is a key enzyme for controlling cell canceration proliferation and is an important factor of tumor growth, the tumor growth process needs to be predicted through cell culture in blood for early prevention and treatment and avoiding tumor formation, when the cells are treated, the cells need to be sent into a laboratory for culture, the cell growth process and lesion are tested, and a new tumor cell population is obtained through cell culture to carry out repeated and intensive research, so that a medicinal reagent and the like capable of aiming at the tumor cells are researched.

The prior Chinese patent CN108753614A discloses a tumor cell culture device for anti-tumor experiments, which uses a first motor to drive a driving gear to rotate, the driving gear drives a driven gear to rotate and further drives a placing disc to rotate, so as to uniformly and stably shake the culture solution in a culture bottle, and simultaneously, starts a stirring blade in the culture bottle to further uniformly stir the culture solution, thereby reducing the labor intensity of medical staff, avoiding the error of the detection result caused by uneven shaking, and automatically carrying out accurate regulation and control of the temperature and humidity in the box body, overcome when handling the cell, need send into the laboratory and cultivate, test cell growth process and pathological change, need medical staff to rock the cell in the culture dish among the culture process, let the cell even growth, because manual operation is difficult to avoid the strength inequality, the problem of error is caused to inspection operation afterwards, but stir the culture solution of blake bottle inside because it adopts stirring vane, and then lead to its cell colony to rotate the in-process cell structure at the flabellum and receive destruction, the flabellum can carry during microorganism gets into the culture solution simultaneously, influence culture result and efficiency.

The chinese patent CN207672074U is directed to the existing culture bottle, however, the current culture bottle is inconvenient for the transplantation of the cut small pieces of tumor tissue due to the inclined neck, and the operation is further affected by the interval between the cut small pieces of tumor tissue, and the smearing of the collagen thin layer is also troublesome. In addition, in the operation engineering, the culture bottle needs to be repeatedly rotated, the prior art generally uses a clamp fastened by screws for positioning the culture bottle, the screws need to be loosened when the culture bottle is rotated, the screws need to be screwed when the culture bottle is positioned, and the problem of inconvenient use is solved, and the tumor cell culture device is disclosed, wherein a bottle mouth cover is arranged on a bevel neck cover of the culture bottle, the bottom of the culture bottle is open, a piston is fixed at one end of a push-pull rod, an annular cotton ring is bonded and sleeved on the piston, the annular cotton ring is plugged in the culture bottle, the bottom cover is fixed at the other end of the push-pull rod, a circular through groove for plugging the culture bottle is arranged in the center of a rubber block, the rubber block is fixed between two shaft rods, the outer ends of the two shaft rods respectively rotate in circular holes at the upper ends of the two vertical plates, a base is fixed at the lower ends of the two vertical plates, a first end gear, the pressure spring is supported between the second end face gear and the vertical plates, and each vertical plate is fixed with two limiting rod heads which are respectively inserted into the two limiting holes of the corresponding second end face gear at intervals. The operation is simple, the culture bottle is convenient to rotate and position, the convenient and fast mode overcomes the defects that the traditional culture bottle is not convenient for the moving in of small blocks cut into the tumor tissue due to the inclined neck, the operation is more influenced by the interval between the small blocks cut into the tumor tissue, and the collagen thin layer is also very troublesome to paint. In addition, in operation engineering, need rotate the blake bottle repeatedly, fix a position the anchor clamps that use screw fastening to the blake bottle usually at present, need loosen the screw during rotatory blake bottle, need screw the screw during location blake bottle, awkward problem, but because this device is in the use, the primary function is fixed blake bottle, follow-up operation still needs artifical the completion, complex operation to the collagen thin layer covers evenly inadequately.

To sum up, need research and develop the even all-round cover of collagen thin layer that can realize tumor cell blake bottle at present, the aseptic interpolation of culture solution, and in the culture process, the regular of blake bottle rocks, nutrient substance's evenly distributed blake bottle preprocessing device promptly, overcome that can't regularly evenly rock the blake bottle among the prior art and make blake bottle nutrient substance evenly distributed, the unable all-round even cover blake bottle inner wall of collagen thin layer, and can't guarantee aseptic interpolation culture solution, and the problem of stirring in-process destruction tumor cell colony structure.

Disclosure of Invention

The invention aims to overcome the defects that nutrient substances of a culture bottle cannot be uniformly distributed by regularly and uniformly shaking the culture bottle, a collagen thin layer cannot uniformly cover the inner wall of the culture bottle in all directions, sterile culture solution cannot be ensured to be added, and the structure of a tumor cell population is damaged in the stirring process in the prior art, and the technical problem to be solved by the invention is to provide a pretreatment device for the tumor cell culture bottle.

The invention is achieved by the following specific technical means:

a tumor cell culture bottle pretreatment device comprises a right bottom frame, a real-time control screen, a left bottom frame, an inner support plate, a sterilization ventilation net, a top curved plate, a collagen thin layer covering mechanism, a sterile sealing adding mechanism and a dead-angle-free rotary culture mechanism; a real-time control screen is arranged in the middle of the right end of the right bottom frame; the bottom of the left end of the right bottom frame is connected with the left bottom frame; the left side of the bottom end of the right bottom frame is connected with the inner supporting plate, and the top of the left end of the inner supporting plate is connected with the left bottom frame; a collagen thin layer covering mechanism is arranged on the left side of the bottom end of the right bottom frame, and the top of the left end of the collagen thin layer covering mechanism is connected with the inner supporting plate; the right side of the bottom end in the right bottom frame is provided with a sterile sealing adding mechanism, and the middle part of the left end of the sterile sealing adding mechanism is connected with a collagen thin layer covering mechanism; a sterilization ventilation net is arranged on the middle left side of the top end of the left bottom frame; the right side of the top end of the left bottom frame is connected with the top curved plate, the bottom end of the top curved plate is connected with the sterile sealing adding mechanism, and the right end of the top curved plate is connected with the right bottom frame; the inside no dead angle rotary culture mechanism that is provided with of left underframe to no dead angle rotary culture mechanism right-hand member top is connected with the inner supporting plate, and no dead angle rotary culture mechanism right-hand member middle part is connected with collagen thin layer cover mechanism.

Further, the collagen thin layer covering mechanism comprises a first special-shaped support, a motor, a first driving wheel, a second driving wheel, a first flat gear, a third driving wheel, a second flat gear, a first electric push rod, a third flat gear, a constant-speed rotating frame wheel, a first bearing sleeve, a first culture bottle fixing frame, a second culture bottle fixing frame and a second bearing sleeve; the top end of the first special-shaped support is provided with a motor; the middle part of the right end of the motor is rotationally connected with the first driving wheel; the lower part of the first driving wheel is in transmission connection with the second driving wheel; the middle part of the right end of the second driving wheel is rotationally connected with the first flat gear; the middle part of the left end of the second driving wheel is rotationally connected with a third driving wheel, and the middle part of the left end of the third driving wheel is connected with a first special-shaped support; a second flat gear is arranged at the lower left of the first flat gear; the middle part of the left end of the second flat gear is rotationally connected with the first electric push rod; a third flat gear is arranged at the right lower part of the second flat gear; the middle part of the right end of the third horizontal gear is rotationally connected with the uniform-speed rotating frame wheel through a rotating shaft rod; the left side of the outer surface of the constant-speed rotating frame wheel is rotationally connected with the first bearing sleeve; the right side of the outer surface of the constant-speed rotating frame wheel is rotationally connected with a second bearing sleeve; a first culture bottle fixing frame is arranged at the top end in the constant-speed rotating frame wheel; a second culture bottle fixing frame is arranged at the bottom end in the constant-speed rotating frame wheel; the middle part of the left end of the first special-shaped support is connected with the inner supporting plate; the left end of the first electric push rod is connected with the inner supporting plate; the bottom end of the first bearing sleeve is connected with the right bottom frame; the bottom end of the second bearing sleeve is connected with the right bottom frame; the middle part of the right end of the first driving wheel is connected with a sterile sealing adding mechanism; the lower part of the third driving wheel is connected with a rotary cultivation mechanism without dead angles.

Further, the sterile sealing adding mechanism comprises a first electric slide rail, a second electric slide rail, a culture solution adding mechanism, a third electric slide rail, a first culture bottle fixing rectangular plate, a fixed workbench, a first lifting slide ring, a first inner rail telescopic rod, a second inner rail telescopic rod, a first screw rod, a workbench supporting plate, a third bearing sleeve, a fourth flat gear, a fourth driving wheel, a first bevel gear, a first gear frame, a second bevel gear, a first long gear, a fifth flat gear, a second long gear, a sixth flat gear, a second electric push rod, a third electric push rod, a fifth driving wheel, a sixth driving wheel, a seventh flat gear, a second gear frame and an eighth flat gear; the middle part of the right end of the first electric slide rail is connected with the second electric slide rail in a sliding way; the middle part of the front end of the second electric slide rail is in sliding connection with the culture solution adding mechanism; the middle part of the right end of the second electric slide rail is connected with a third electric slide rail in a sliding way; a first culture bottle fixing rectangular plate is arranged below the culture solution adding mechanism; the bottom end of the first culture bottle fixing rectangular plate is connected with a fixing workbench; a first lifting slip ring is arranged in the middle of the bottom end of the fixed workbench; the left side of the bottom end of the fixed workbench is connected with a first inner rail telescopic rod; the right side of the bottom end of the fixed workbench is connected with a second inner rail telescopic rod; the inner middle part of the bottom end of the first lifting slip ring is rotationally connected with the first screw rod; the bottom end of the second inner rail telescopic rod is connected with the workbench supporting plate, and the left side of the top end of the workbench supporting plate is connected with the first inner rail telescopic rod; a third bearing sleeve is arranged in the middle of the bottom end of the workbench supporting plate, and the middle of the bottom end in the third bearing sleeve is connected with the first screw rod; a fourth transmission wheel is arranged in the left middle of the bottom end of the workbench supporting plate; the middle right side of the bottom end of the workbench supporting plate is rotationally connected with the first long gear; the middle part of the bottom end of the third bearing sleeve is rotationally connected with the fourth flat gear; the left side of the fourth transmission wheel is in transmission connection with the first bevel gear; the middle part of the bottom end of the fourth driving wheel is rotationally connected with the second long gear; the middle part of the bottom end of the first bevel gear is rotationally connected with the first gear carrier; the middle part of the right end of the first bevel gear is meshed with the second bevel gear, and the middle part of the left end of the second bevel gear is connected with the first gear rack; the middle part of the left end of the first long gear is meshed with the fifth flat gear; the middle part of the bottom end of the first long gear is rotationally connected with a fifth transmission wheel; the middle part of the bottom end of the fifth flat gear is rotationally connected with a third electric push rod; the middle part of the right end of the second long gear is meshed with the sixth flat gear; the middle part of the bottom end of the sixth flat gear is rotationally connected with the second electric push rod; the left side of the fifth driving wheel is in transmission connection with the sixth driving wheel; the middle part of the top end of the sixth transmission wheel is rotationally connected with the seventh flat gear; the middle part of the top end of the seventh flat gear is rotationally connected with the second gear rack; the middle part of the right end of the seventh flat gear is meshed with the eighth flat gear, the middle part of the top end of the eighth flat gear is connected with the second long gear, and the middle part of the bottom end of the eighth flat gear is connected with the second gear rack; the top end of the first electric slide rail is connected with the top curved plate; the top end of the second electric sliding rail is connected with the top curved plate; the top end of the third electric slide rail is connected with the top curved plate; the top end of the first gear rack is connected with the top curved plate; the middle part of the bottom end of the sixth transmission wheel is connected with the right bottom frame; the bottom end of the second electric push rod is connected with the right bottom frame; the bottom end of the third electric push rod is connected with the right bottom frame; the middle part of the bottom end of the fifth driving wheel is connected with the right bottom frame; the middle part of the left end of the second bevel gear is connected with the first transmission wheel.

Further, the dead-corner-free rotary culture mechanism comprises a second culture bottle fixing circular plate, a universal ball, a universal bearing seat, a telescopic connecting rod, a fourth electric push rod, a fixing circular table plate, a ninth flat gear, a tenth flat gear, a fifth electric push rod, an eleventh flat gear, a seventh driving wheel, a third bevel gear, a third gear carrier and a fourth bevel gear; the middle part of the bottom end of the second culture bottle fixing circular plate is rotatably connected with the universal ball; the outer surface of the universal ball is rotationally connected with the universal bearing seat; the middle part of the bottom end of the universal ball is connected with a telescopic connecting rod; the inner part of the bottom end of the telescopic connecting rod is connected with a fourth electric push rod; the bottom end of the fourth electric push rod is welded with the fixed round table plate; the middle part of the bottom end of the fixed round bedplate is rotationally connected with the ninth flat gear; a tenth flat gear is arranged at the right lower part of the ninth flat gear; the middle part of the bottom end of the tenth flat gear is rotationally connected with the fifth electric push rod; an eleventh flat gear is arranged at the right upper part of the tenth flat gear; the middle part of the top end of the eleventh flat gear is rotationally connected with a seventh transmission wheel; the right side of the seventh transmission wheel is in transmission connection with the third bevel gear; the middle part of the bottom end of the third bevel gear is rotationally connected with a third gear rack; the middle part of the right end of the third gear frame is meshed with a fourth bevel gear, and the middle part of the right end of the fourth bevel gear is connected with the third gear frame; the left end of the universal bearing seat is connected with the left bottom frame; the right end of the universal bearing seat is connected with the inner supporting plate; the right end of the third gear rack is connected with the right bottom frame; the middle part of the bottom end of the ninth flat gear is connected with the left bottom frame; the bottom end of the fifth electric push rod is connected with the left bottom frame; the middle part of the bottom end of the eleventh flat gear is connected with the left bottom frame; the upper part of the fourth bevel gear is connected with a third driving wheel.

Further, the first culture bottle fixing frame comprises a left wheel fixing plate, an inner inclined plate, a right wheel fixing plate, a fixing box, a pressing fixing plate, a second screw rod and a screwing hand wheel; the bottom of the right end of the left wheel fixing plate is connected with an inner inclined plate; the right end of the inner inclined plate is connected with the right wheel fixing plate; the middle part of the top end of the inner inclined plate is connected with the fixed box; a pressing fixing plate is arranged at the top end inside the fixing box; the inner side of the middle part of the top end of the fixed box is rotationally connected with a second screw rod, and the bottom end of the second screw rod is connected with a pressing fixed plate; the top end of the second screw rod is welded with a screwing hand wheel; the middle upper part of the left end of the left wheel fixing plate is connected with a uniform speed rotating frame wheel; the middle-lower part of the right end of the right wheel fixing plate is connected with a uniform rotating frame wheel.

Further, the culture solution adding mechanism comprises a ground opening cover, a culture solution tank, a rear fixed sliding plate, a first liquid dropping pipe, a wrench switch, an angle adjusting hand wheel, a second bent liquid dropping pipe, a bottle inlet pipe and a sealing edge cover; the outer surface of the grinding cover is connected with a culture solution tank; the rear side of the outer surface of the culture solution tank is connected with the rear fixed sliding plate; the middle part of the bottom end of the culture solution tank is connected with a first down liquid pipe; a spanner switch is arranged at the middle upper part of the left end of the first lower liquid pipe; the bottom of the front end of the first lower liquid pipe is connected with an angle adjusting hand wheel; the bottom end of the first liquid descending pipe is connected with the second bent liquid descending pipe, and the left side of the front end of the second bent liquid descending pipe is connected with the angle adjusting hand wheel; the middle part of the right end of the second bending liquid dropping pipe is connected with the bottle inlet pipe; the middle part of the outer surface of the bottle inlet pipe is connected with the sealing edge cover; the middle part of the rear end of the rear fixed sliding plate is connected with a second electric sliding rail.

Further, the first culture bottle fixing frame and the second culture bottle fixing frame are respectively at thirty degrees and three hundred thirty degrees with the positive direction of the horizontal X axis.

Furthermore, the left end and the right end of the fixed workbench are respectively provided with an arc-shaped groove plate.

Compared with the prior art, the invention has the following beneficial effects:

in order to solve the problems that nutrient substances of a culture bottle cannot be uniformly distributed by regularly and uniformly shaking the culture bottle, a collagen thin layer cannot be uniformly covered on the inner wall of the culture bottle in all directions, sterile culture solution can not be ensured to be added, and the structure of a tumor cell population is damaged in the stirring process in the prior art, the method designs a collagen thin layer covering mechanism, a sterile sealing adding mechanism and a dead-angle-free rotating culture mechanism, when in use, firstly, the collagen thin layer covering mechanism is used for uniformly covering the collagen thin layer on the inner wall of the culture bottle, then, the sterile sealing adding mechanism is used for adding the culture solution into the culture bottle in a sealed sterile environment, then, the dead-angle-free rotating culture mechanism is used for regularly and uniformly shaking in the tumor cell culture process, so that the uniform and all-directional covering of the collagen thin layer of the tumor cell culture bottle, the sterile adding of the culture solution and, namely the effect of the culture bottle pretreatment device with the uniform distribution of nutrient substances.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the structure of the thin collagen layer covering mechanism of the present invention;

FIG. 3 is a schematic structural view of a sterile seal adding mechanism of the present invention;

FIG. 4 is a schematic structural view of a blind-corner-free rotary culturing mechanism according to the present invention;

FIG. 5 is a schematic view of a first flask holder according to the present invention;

FIG. 6 is a schematic view of a structure of a culture solution adding mechanism according to the present invention;

fig. 7 is a schematic view of the structure of the bottle inlet pipe and the sealing edge cover of the invention.

The labels in the figures are: 1-right bottom frame, 2-real-time control screen, 3-left bottom frame, 4-inner support plate, 5-sterilization ventilation net, 6-top curved plate, 7-collagen thin layer covering mechanism, 8-sterile sealing adding mechanism, 9-dead angle-free rotary culture mechanism, 701-first special-shaped support, 702-motor, 703-first driving wheel, 704-second driving wheel, 705-first flat gear, 706-third driving wheel, 707-second flat gear, 708-first electric push rod, 709-third flat gear, 7010-rotary constant-speed rotating frame wheel, 7011-first bearing sleeve, 7012-first culture bottle fixing frame, 7013-second culture bottle fixing frame, 7014-second bearing sleeve, 801-first electric slide rail, 802-second electric slide rail, 803-culture solution adding mechanism, 804-third electric sliding rail, 805-first culture bottle fixed rectangular plate, 806-fixed workbench, 807-first lifting sliding ring, 808-first inner rail telescopic rod, 809-second inner rail telescopic rod, 8010-first screw rod, 8011-workbench supporting plate, 8012-third bearing sleeve, 8013-fourth flat gear, 8014-fourth driving wheel, 8015-first bevel gear, 8016-first gear rack, 8017-second bevel gear, 8018-first long gear, 8019-fifth flat gear, 8020-second long gear, 8021-sixth flat gear, 8022-second electric push rod, 8023-third electric push rod, 8024-fifth driving wheel, 8025-sixth driving wheel, 8026-seventh flat gear, 8027-second gear rack, 8028-eighth flat gear, 901-second culture bottle fixing circular plate, 902-universal ball, 903-universal bearing seat, 904-telescopic connecting rod, 905-fourth electric push rod, 906-fixing circular table plate, 907-ninth flat gear, 908-tenth flat gear, 909-fifth electric push rod, 9010-eleventh flat gear, 9011-seventh driving wheel, 9012-third bevel gear, 9013-third gear frame, 9014-fourth bevel gear, 701201-left wheel fixing plate, 701202-inner inclined plate, 701203-right wheel fixing plate, 701204-fixing box, 701205-pressing fixing plate, 701206-second lead screw, 701207-screwing hand wheel, 80301-grinding cover, 80302-culture liquid tank, 80303-rear fixing sliding plate, 80304-first down liquid pipe, 80305-wrench switch, 80306-angle adjusting handwheel, 80307-second bending liquid dropping pipe, 80308-bottle inlet pipe, 80309-sealing edge cover.

Detailed Description

The invention is further described below with reference to the figures and examples.

Examples

A tumor cell culture bottle pretreatment device is shown in figures 1-7 and comprises a right bottom frame 1, a real-time control screen 2, a left bottom frame 3, an inner supporting plate 4, a sterilization ventilation net 5, a top curved plate 6, a collagen thin layer covering mechanism 7, a sterile sealing adding mechanism 8 and a dead-angle-free rotary culture mechanism 9; a real-time control screen 2 is arranged in the middle of the right end of the right bottom frame 1; the bottom of the left end of the right bottom frame 1 is connected with the left bottom frame 3; the left side of the bottom end in the right bottom frame 1 is connected with an inner support plate 4, and the top of the left end of the inner support plate 4 is connected with a left bottom frame 3; a collagen thin layer covering mechanism 7 is arranged on the left side of the bottom end in the right bottom frame 1, and the top of the left end of the collagen thin layer covering mechanism 7 is connected with the inner supporting plate 4; the right side of the bottom end in the right bottom frame 1 is provided with a sterile sealing adding mechanism 8, and the middle part of the left end of the sterile sealing adding mechanism 8 is connected with a collagen thin layer covering mechanism 7; a sterilization ventilation net 5 is arranged on the left side in the top end of the left bottom frame 3; the right side of the top end of the left bottom frame 3 is connected with a top curved plate 6, the bottom end of the top curved plate 6 is connected with a sterile sealing adding mechanism 8, and the right end of the top curved plate 6 is connected with the right bottom frame 1; the inside no dead angle rotary culture mechanism 9 that is provided with of left underframe 3 to no dead angle rotary culture mechanism 9 right-hand member top is connected with inner supporting plate 4, and no dead angle rotary culture mechanism 9 right-hand member middle part is connected with collagen thin layer covering mechanism 7.

The collagen thin layer covering mechanism 7 comprises a first special-shaped support 701, a motor 702, a first driving wheel 703, a second driving wheel 704, a first flat gear 705, a third driving wheel 706, a second flat gear 707, a first electric push rod 708, a third flat gear 709, a uniform speed rotating rack wheel 7010, a first bearing sleeve 7011, a first culture bottle fixing rack 7012, a second culture bottle fixing rack 7013 and a second bearing sleeve 7014; the top end of the first special-shaped support 701 is provided with a motor 702; the middle part of the right end of the motor 702 is rotatably connected with a first driving wheel 703; the lower part of the first driving wheel 703 is in transmission connection with a second driving wheel 704; the middle part of the right end of the second transmission wheel 704 is rotationally connected with a first flat gear 705; the middle part of the left end of the second driving wheel 704 is rotationally connected with a third driving wheel 706, and the middle part of the left end of the third driving wheel 706 is connected with a first special-shaped support 701; a second pinion 707 is arranged below the first pinion 705 on the left; the middle part of the left end of the second flat gear 707 is rotationally connected with the first electric push rod 708; a third spur gear 709 is arranged at the lower right of the second spur gear 707; the middle part of the right end of the third spur gear 709 is rotatably connected with a uniform rotating frame wheel 7010 through a rotating shaft rod; the left side of the outer surface of the uniform-speed rotating frame wheel 7010 is rotationally connected with a first bearing sleeve 7011; the right side of the outer surface of the uniform-speed rotating frame wheel 7010 is rotationally connected with a second bearing sleeve 7014; a first culture bottle fixing rack 7012 is arranged at the top end in the uniform-speed rotating rack wheel 7010; a second culture bottle fixing rack 7013 is arranged at the inner bottom end of the uniform-speed rotating rack wheel 7010; the middle part of the left end of the first special-shaped support 701 is connected with the inner support plate 4; the left end of the first electric push rod 708 is connected with the inner supporting plate 4; the bottom end of the first bearing sleeve 7011 is connected with the right bottom frame 1; the bottom end of the second bearing sleeve 7014 is connected with the right bottom frame 1; the middle part of the right end of the first driving wheel 703 is connected with a sterile sealing adding mechanism 8; the lower part of the third driving wheel 706 is connected with the dead-angle-free rotary culture mechanism 9.

The sterile sealing adding mechanism 8 comprises a first electric sliding rail 801, a second electric sliding rail 802, a culture solution adding mechanism 803, a third electric sliding rail 804, a first culture bottle fixing rectangular plate 805, a fixed working platform 806, a first lifting sliding ring 807, a first inner rail telescopic rod 808, a second inner rail telescopic rod 809, a first screw rod 8010, a working platform supporting plate 8011, a third bearing sleeve 8012, a fourth flat gear 8013, a fourth driving wheel 8014, a first bevel gear 8015, a first gear frame 8016, a second bevel gear 8017, a first long gear 8018, a fifth flat gear 8019, a second long gear 8020, a sixth flat gear 8021, a second electric push rod 8022, a third electric push rod 8023, a fifth driving wheel 8024, a sixth driving wheel 8025, a seventh flat gear 8026, a second gear frame 8027 and an eighth flat gear 8028; the middle part of the right end of the first electric slide rail 801 is connected with the second electric slide rail 802 in a sliding manner; the middle part of the front end of the second electric slide rail 802 is connected with the culture solution adding mechanism 803 in a sliding way; the middle part of the right end of the second electric sliding rail 802 is connected with a third electric sliding rail 804 in a sliding manner; a first culture bottle fixing rectangular plate 805 is arranged below the culture solution adding mechanism 803; the bottom end of the first flask fixing rectangular plate 805 is connected with a fixing workbench 806; a first lifting slip ring 807 is arranged in the middle of the bottom end of the fixed workbench 806; the left side of the bottom end of the fixed workbench 806 is connected with a first inner rail telescopic rod 808; the right side of the bottom end of the fixed workbench 806 is connected with a second inner rail telescopic rod 809; the inner middle part of the bottom end of the first lifting slip ring 807 is rotatably connected with the first screw rod 8010; the bottom end of the second inner rail telescopic rod 809 is connected with the workbench supporting plate 8011, and the left side of the top end of the workbench supporting plate 8011 is connected with the first inner rail telescopic rod 808; a third bearing sleeve 8012 is arranged in the middle of the bottom end of the workbench supporting plate 8011, and the middle of the bottom end in the third bearing sleeve 8012 is connected with the first screw rod 8010; a fourth driving wheel 8014 is arranged in the left middle of the bottom end of the workbench supporting plate 8011; the middle right side of the bottom end of the workbench supporting plate 8011 is rotatably connected with the first long gear 8018; the middle part of the bottom end of the third bearing sleeve 8012 is rotatably connected with the fourth flat gear 8013; the left side of the fourth driving wheel 8014 is in driving connection with a first bevel gear 8015; the middle part of the bottom end of the fourth driving wheel 8014 is rotatably connected with a second long gear 8020; the middle part of the bottom end of the first bevel gear 8015 is rotatably connected with the first gear rack 8016; the middle part of the right end of the first bevel gear 8015 meshes with the second bevel gear 8017, and the middle part of the left end of the second bevel gear 8017 is connected with the first gear carrier 8016; the middle of the left end of the first long gear 8018 meshes with the fifth flat gear 8019; the middle part of the bottom end of the first long gear 8018 is rotatably connected with a fifth driving wheel 8024; the middle part of the bottom end of the fifth flat gear 8019 is rotatably connected with a third electric push rod 8023; the middle part of the right end of the second long gear 8020 is engaged with a sixth flat gear 8021; the middle part of the bottom end of the sixth flat gear 8021 is rotatably connected with a second electric push rod 8022; the left side of the fifth driving wheel 8024 is in transmission connection with a sixth driving wheel 8025; the middle part of the top end of the sixth transmission wheel 8025 is rotatably connected with a seventh flat gear 8026; the middle part of the top end of the seventh flat gear 8026 is rotationally connected with the second gear carrier 8027; the middle part of the right end of the seventh flat gear 8026 is engaged with the eighth flat gear 8028, the middle part of the top end of the eighth flat gear 8028 is connected with the second long gear 8020, and the middle part of the bottom end of the eighth flat gear 8028 is connected with the second gear carrier 8027; the top end of the first electric sliding rail 801 is connected with the top curved plate 6; the top end of the second electric slide rail 802 is connected with the top curved plate 6; the top end of the third electric sliding rail 804 is connected with the top curved plate 6; the top end of the first gear carrier 8016 is connected with the top curved plate 6; the middle part of the bottom end of the sixth transmission wheel 8025 is connected with the right bottom frame 1; the bottom end of a second electric push rod 8022 is connected with the right bottom frame 1; the bottom end of a third electric push rod 8023 is connected with the right bottom frame 1; the middle part of the bottom end of the fifth transmission wheel 8024 is connected with the right bottom frame 1; the middle of the left end of the second bevel gear 8017 is connected to the first driving wheel 703.

The dead-corner-free rotary culture mechanism 9 comprises a second culture bottle fixing circular plate 901, a universal ball 902, a universal bearing seat 903, a telescopic connecting rod 904, a fourth electric push rod 905, a fixing circular plate 906, a ninth flat gear 907, a tenth flat gear 908, a fifth electric push rod 909, an eleventh flat gear 9010, a seventh driving wheel 9011, a third bevel gear 9012, a third gear carrier 9013 and a fourth bevel gear 9014; the middle part of the bottom end of the second culture bottle fixing circular plate 901 is rotatably connected with the universal ball 902; the outer surface of the universal ball 902 is rotatably connected with a universal bearing seat 903; the middle part of the bottom end of the universal ball 902 is connected with a telescopic connecting rod 904; the inner part of the bottom end of the telescopic connecting rod 904 is connected with a fourth electric push rod 905; the bottom end of a fourth electric push rod 905 is welded with the fixed round table plate 906; the middle part of the bottom end of the fixed round bedplate 906 is rotationally connected with a ninth flat gear 907; a tenth spur gear 908 is arranged at the lower right of the ninth spur gear 907; the middle part of the bottom end of the tenth flat gear 908 is rotatably connected with a fifth electric push rod 909; an eleventh flat gear 9010 is arranged above the right of the tenth flat gear 908; the middle part of the top end of the eleventh flat gear 9010 is rotatably connected with a seventh transmission wheel 9011; the right side of the seventh transmission wheel 9011 is in transmission connection with a third bevel gear 9012; the middle part of the bottom end of the third bevel gear 9012 is rotatably connected with a third gear carrier 9013; the middle part of the right end of the third gear frame 9013 is meshed with the fourth bevel gear 9014, and the middle part of the right end of the fourth bevel gear 9014 is connected with the third gear frame 9013; the left end of the universal bearing seat 903 is connected with the left bottom frame 3; the right end of the universal bearing seat 903 is connected with the inner supporting plate 4; the right end of a third gear rack 9013 is connected with the right bottom frame 1; the middle part of the bottom end of the ninth spur gear 907 is connected with the left bottom frame 3; the bottom end of the fifth electric push rod 909 is connected with the left bottom frame 3; the middle part of the bottom end of the eleventh flat gear 9010 is connected with the left bottom frame 3; the upper part of the fourth bevel gear 9014 is connected with a third driving wheel 706.

The first flask fixing rack 7012 comprises a left wheel fixing plate 701201, an inner inclined plate 701202, a right wheel fixing plate 701203, a fixing box 701204, a pressing fixing plate 701205, a second screw rod 701206 and a screwing hand wheel 701207; the bottom of the right end of the left wheel fixing plate 701201 is connected with an inner sloping plate 701202; the right end of the inner inclined plate 701202 is connected with a right wheel fixing plate 701203; the middle part of the top end of the inner inclined plate 701202 is connected with the fixed box 701204; a pressing fixing plate 701205 is arranged at the top end inside the fixing box 701204; the inner side of the middle part of the top end of the fixed box 701204 is rotatably connected with a second screw rod 701206, and the bottom end of the second screw rod 701206 is connected with a pressing fixed plate 701205; the top end of the second screw 701206 is welded with a screwing hand wheel 701207; the middle upper part of the left end of the left wheel fixing plate 701201 is connected with a uniform speed rotating frame wheel 7010; the middle-lower part of the right end of the right wheel fixing plate 701203 is connected with a uniform speed rotating frame wheel 7010.

The culture solution adding mechanism 803 comprises a grinding opening cover 80301, a culture solution tank 80302, a rear fixed sliding plate 80303, a first liquid dropping pipe 80304, a spanner switch 80305, an angle adjusting hand wheel 80306, a second bent liquid dropping pipe 80307, a bottle inlet pipe 80308 and a sealing edge cover 80309; the outer surface of the grinding opening cover 80301 is connected with a culture solution tank 80302; the rear side of the outer surface of the culture solution tank 80302 is connected with a rear fixed sliding plate 80303; the middle part of the bottom end of the culture solution tank 80302 is connected with a first lower liquid pipe 80304; a wrench switch 80305 is arranged at the middle upper part of the left end of the first lower liquid pipe 80304; the bottom of the front end of the first lower liquid pipe 80304 is connected with an angle adjusting handwheel 80306; the bottom end of the first liquid descending pipe 80304 is connected with the second bent liquid descending pipe 80307, and the left side of the front end of the second bent liquid descending pipe 80307 is connected with an angle adjusting hand wheel 80306; the middle part of the right end of the second bending liquid dropping pipe 80307 is connected with a bottle inlet pipe 80308; the middle part of the outer surface of the bottle inlet tube 80308 is connected with a sealing edge cover 80309; the middle part of the rear end of the rear fixed sliding plate 80303 is connected with a second electric sliding rail 802.

The first flask mount 7012 and the second flask mount 7013 are at thirty degrees and three hundred thirty degrees, respectively, from the horizontal X axis forward.

The left end and the right end of the fixed workbench 806 are respectively provided with an arc-shaped groove plate.

The working principle is as follows: when the tumor cell culture bottle pretreatment device is used, firstly, the device is fixed in a working plane of a sterile biochemical laboratory, then sufficient tumor cell culture solution in a sterile sealed adding mechanism 8 is added, then a power supply is externally connected, firstly, a cell culture bottle to be used is fixed in a collagen thin layer covering mechanism 7, sufficient collagen is added in each bottle, the power supply is externally connected, a real-time control screen 2 is manually opened, the device starts to operate, then, the collagen thin layer covering mechanism 7 is internally covered by the collagen thin layer on the inner wall of the cell culture bottle, then, the cell culture bottle is fixed in the sterile sealed adding mechanism 8 for the next culture solution adding, after the culture solution is added, an experimenter adds tumor primary generation cells needing culture research into the culture bottle after the treatment, then, the culture bottle is fixed in a dead-corner-free rotary culture mechanism 9, regularly carry out the blake bottle through the control of real-time control screen 2 and rock, disinfect ventilation network 5 simultaneously and can guarantee the exchange of blake bottle and external gas, provide the required oxygen of cell growth, realized the collagen thin layer of tumor cell blake bottle and covered, the aseptic interpolation of culture solution to and in the culture process, the regular of blake bottle rocks, nutrient substance's evenly distributed promptly.

Firstly, an experiment worker adds enough collagen liquid for cell culture into each cell culture bottle, then the top cover of the culture bottle is sealed, then the culture bottles are sequentially fixed inside a first culture bottle fixing frame 7012 and a second culture bottle fixing frame 7013, then the power of a motor 702 is switched on under the control of a real-time control screen 2, then the right end of the motor 702 drives a first driving wheel 703 to rotate, then the lower part of the first driving wheel 703 drives a second driving wheel 704 to rotate through a belt, then the right end of the second driving wheel 704 drives a first flat gear 705 to rotate, further a first electric push rod 708 is controlled to be pushed out rightwards through the real-time control screen 2, then the right end of the first electric push rod 708 drives the top end and the bottom end of a second flat gear 707 to be respectively meshed with the first flat gear 705 and a third flat gear 709, then the bottom end of the first flat gear 705 further drives the third flat, then the right end of the third spur gear 709 drives the uniform rotating rack wheel 7010 to rotate through the rotating shaft rod, and then the collagen liquid inside the culture bottle is fully contacted with the inner wall of the culture bottle in the process that the cell culture bottle fixed inside the first culture bottle fixing frame 7012 and the second culture bottle fixing frame 7013 are turned over up and down, namely, the inner wall of the culture bottle is uniformly covered with a collagen layer, and the covering of the collagen thin layer on the inner wall of the cell culture bottle is completed.

After the collagen thin layer on the inner wall of the culture bottle is completely covered, all the culture bottles are fixed inside a first culture bottle fixing rectangular plate 805 at the top end of a fixed workbench 806, then the middle part of the right end of a first driving wheel 703 drives a second bevel gear 8017 to rotate through a rotating shaft, further the middle part of the bottom end of the second bevel gear 8017 drives a first bevel gear 8015 to rotate, then the right side of the first bevel gear 8015 drives a fourth driving wheel 8014 to rotate through a belt, then the middle part of the bottom end of the fourth driving wheel 8014 drives a second long gear 8020 to rotate, further the middle part of the right end of the second long gear 8020 drives a sixth flat gear 8021 engaged with the second long gear 8020 to rotate, meanwhile, the bottom end of the second long gear 8020 drives an eighth flat gear 8028 to rotate, further the left end of the eighth flat gear 8028 rotates through a seventh flat gear 8026, further the middle part of the bottom end of the seventh flat gear 8026 drives a sixth driving wheel 8025 to rotate, then the top end of a fifth driving wheel 8024 drives a fifth flat gear 8019 to rotate, then the real-time control panel 2 controls a second electric push rod 8022 to push out upwards, and then the second electric push rod 8022 drives the right end of a sixth flat gear 8021 to engage with the fourth flat gear 8013 to rotate, and then the fourth flat gear 8013 drives a first lead screw 8010 at the top end to rotate, and then the first lead screw 8010 rotates, under the action that the first lifting slip ring 807 cooperates with the first lead screw 8010 to rotate, the first lifting slip ring 807 drives a fixed workbench 806 to move upwards, that is, the first long gear 8018 rotates backwards, that is, the first lead screw 8010 rotates backwards to control the positions of the first culture bottle fixed moment plate 805 and the fixed workbench 806 to lower, then the top cover of the culture bottle is taken down, a sufficient amount of culture solution is added into the culture solution adding mechanism 803, then the first electric slide rail 801, the second electric slide rail 802 and the third electric slide rail 804 jointly control the culture solution adding mechanism 803 to move to a proper position, namely the culture solution adding mechanism 803 extends into the culture bottle, a sufficient amount of cell culture solution is added into each culture bottle under a sealed condition, and the addition of sterile culture solution of the cell culture bottle is completed.

Firstly, adding tumor cells to be cultured into a culture bottle filled with a culture solution, fixing the culture bottle to the top end of a second culture bottle fixing circular plate 901, driving a fourth bevel gear 9014 to rotate through a belt below a third driving wheel 706, driving a third bevel gear 9012 to rotate at the middle part of the bottom end of the fourth bevel gear 9014, driving a seventh driving wheel 9011 to rotate through the belt at the left side of the third bevel gear 9012, driving an eleventh flat gear 9010 to rotate at the bottom end of the second culture bottle fixing circular plate 901, setting a timing time through a real-time control screen 2, automatically controlling a fifth electric push rod 909 to push out upwards at regular intervals, driving the left end and the right end of a tenth flat gear 908 to be respectively meshed with a ninth flat gear 907 and the eleventh flat gear 9010 at the top end of the fifth electric push rod 909, driving the ninth flat gear 907 to rotate through the tenth flat gear 908 at the left end of the eleventh flat gear 9010, then the top end of a ninth flat gear 907 drives a fixed circular table plate 906 to rotate, meanwhile, a fourth electric push rod 905 is controlled to push out rightwards, then the fourth electric push rod 905 drives a telescopic connecting rod 904 to incline rightwards, then the top end of the telescopic connecting rod 904 drives a universal ball 902 to incline rightwards, further the top end of the universal ball 902 drives a second culture bottle fixed circular plate 901 to incline leftwards, then under the rotating action of the fixed circular table plate 906, the top end of the fixed circular table plate 906 drives the universal ball 902 to rotate inside a universal bearing seat 903 through the telescopic connecting rod 904 and the fourth electric push rod 905, namely the top end of the universal ball 902 drives the second culture bottle fixed circular plate 901 to rotate, then the culture bottle at the top end of the second culture bottle fixed circular plate 901 realizes timing shaking, and the uniform distribution of nutrient substances inside the culture bottle is guaranteed.

Inside at first adding the cell culture bottle with collagen liquid, then covering the lid, with the culture bottle stopper advance fixed casket 701204 inside compress tightly fixed plate 701205 below, then artifical manual rotation hand wheel 701207 of screwing, through second lead screw 701206, constantly drive compress tightly fixed plate 701205 and push down, wait to compress tightly fixed plate 701205 and press from both sides the culture bottle tightly, cell culture bottle no longer removes promptly, has accomplished the slope of cell culture bottle and has fixed.

Firstly, the ground cover 80301 is manually opened, sufficient cell culture solution is added, then the cover is covered, then the direction of the second bending liquid dropping pipe 80307 is controlled by the angle adjusting hand wheel 80306, the bottle entering pipe 80308 extends into the cell culture bottle, the sealing edge cover 80309 enables the mouth of the culture bottle to be sleeved on the inner side, so that the relative sealing performance of the mouth is ensured, a sterile liquid entering state is obtained, and the sterile bottle entering of the culture solution is realized.

First blake bottle mount 7012 and second blake bottle mount 7013 become thirty degrees and three hundred thirty degrees with horizontal X axle forward respectively to be convenient for the inside fixed cell culture bottle of first blake bottle mount 7012 keeps the slope, when first blake bottle is driven to the top by uniform velocity revolving rack wheel 7010, collagen liquid flows to the inside left side of cell culture bottle, then when waiting for first blake bottle to be driven to the bottom by uniform velocity revolving rack wheel 7010, collagen liquid flows to the inside right side of blake bottle, then in the circulation rotation in-process, the all-round evenly distributed of collagen liquid covers to the inner wall of cell culture bottle.

The left end and the right end of the fixed workbench 806 are respectively provided with an arc-shaped groove plate, so that when the culture solution is leaked, the culture solution can be collected in time.

The above description is provided only to illustrate the technical concept of the present invention, and those skilled in the art will appreciate that various changes and modifications can be made without changing the essential features of the present invention. Accordingly, the exemplary embodiments of the present invention are provided for illustrative purposes only, and are not intended to limit the technical concept of the present invention. The scope of the technical idea of the present disclosure is not limited thereto. Therefore, it should be understood that the above-described exemplary embodiments are illustrative in all respects, not restrictive of the disclosure. The scope of the present disclosure should be construed based on the claims, and all technical ideas within the equivalent scope thereof should be construed to fall within the scope of the present disclosure.

Claims

1. A tumor cell culture bottle pretreatment device comprises a right bottom frame, a real-time control screen, a left bottom frame and an inner support plate, and is characterized by further comprising a sterilization ventilation net, a top bent plate, a collagen thin layer covering mechanism, a sterile sealing adding mechanism and a dead-angle-free rotary culture mechanism; a real-time control screen is arranged in the middle of the right end of the right bottom frame; the bottom of the left end of the right bottom frame is connected with the left bottom frame; the left side of the bottom end of the right bottom frame is connected with the inner supporting plate, and the top of the left end of the inner supporting plate is connected with the left bottom frame; a collagen thin layer covering mechanism is arranged on the left side of the bottom end of the right bottom frame, and the top of the left end of the collagen thin layer covering mechanism is connected with the inner supporting plate; the right side of the bottom end in the right bottom frame is provided with a sterile sealing adding mechanism, and the middle part of the left end of the sterile sealing adding mechanism is connected with a collagen thin layer covering mechanism; a sterilization ventilation net is arranged on the middle left side of the top end of the left bottom frame; the right side of the top end of the left bottom frame is connected with the top curved plate, the bottom end of the top curved plate is connected with the sterile sealing adding mechanism, and the right end of the top curved plate is connected with the right bottom frame; the inside no dead angle rotary culture mechanism that is provided with of left underframe to no dead angle rotary culture mechanism right-hand member top is connected with the inner supporting plate, and no dead angle rotary culture mechanism right-hand member middle part is connected with collagen thin layer cover mechanism.

2. The pretreatment device for tumor cell culture flasks of claim 1, wherein the collagen thin layer covering mechanism comprises a first special-shaped support, a motor, a first driving wheel, a second driving wheel, a first flat gear, a third driving wheel, a second flat gear, a first electric push rod, a third flat gear, a constant rotation rack wheel, a first bearing sleeve, a first culture flask fixing rack, a second culture flask fixing rack and a second bearing sleeve; the top end of the first special-shaped support is provided with a motor; the middle part of the right end of the motor is rotationally connected with the first driving wheel; the lower part of the first driving wheel is in transmission connection with the second driving wheel; the middle part of the right end of the second driving wheel is rotationally connected with the first flat gear; the middle part of the left end of the second driving wheel is rotationally connected with a third driving wheel, and the middle part of the left end of the third driving wheel is connected with a first special-shaped support; a second flat gear is arranged at the lower left of the first flat gear; the middle part of the left end of the second flat gear is rotationally connected with the first electric push rod; a third flat gear is arranged at the right lower part of the second flat gear; the middle part of the right end of the third horizontal gear is rotationally connected with the uniform-speed rotating frame wheel through a rotating shaft rod; the left side of the outer surface of the constant-speed rotating frame wheel is rotationally connected with the first bearing sleeve; the right side of the outer surface of the constant-speed rotating frame wheel is rotationally connected with a second bearing sleeve; a first culture bottle fixing frame is arranged at the top end in the constant-speed rotating frame wheel; a second culture bottle fixing frame is arranged at the bottom end in the constant-speed rotating frame wheel; the middle part of the left end of the first special-shaped support is connected with the inner supporting plate; the left end of the first electric push rod is connected with the inner supporting plate; the bottom end of the first bearing sleeve is connected with the right bottom frame; the bottom end of the second bearing sleeve is connected with the right bottom frame; the middle part of the right end of the first driving wheel is connected with a sterile sealing adding mechanism; the lower part of the third driving wheel is connected with a rotary cultivation mechanism without dead angles.

3. The tumor cell culture flask pretreatment device according to claim 2, wherein the sterile seal adding mechanism comprises a first electric slide rail, a second electric slide rail, a culture solution adding mechanism, a third electric slide rail, a first culture flask fixing rectangular plate, a fixed worktable, a first lifting slide ring, a first inner rail telescopic rod, a second inner rail telescopic rod, a first screw rod, a worktable supporting plate, a third bearing sleeve, a fourth flat gear, a fourth transmission wheel, a first bevel gear, a first gear rack, a second bevel gear, a first long gear, a fifth flat gear, a second long gear, a sixth flat gear, a second electric push rod, a third electric push rod, a fifth transmission wheel, a sixth transmission wheel, a seventh flat gear, a second gear rack and an eighth flat gear; the middle part of the right end of the first electric slide rail is connected with the second electric slide rail in a sliding way; the middle part of the front end of the second electric slide rail is in sliding connection with the culture solution adding mechanism; the middle part of the right end of the second electric slide rail is connected with a third electric slide rail in a sliding way; a first culture bottle fixing rectangular plate is arranged below the culture solution adding mechanism; the bottom end of the first culture bottle fixing rectangular plate is connected with a fixing workbench; a first lifting slip ring is arranged in the middle of the bottom end of the fixed workbench; the left side of the bottom end of the fixed workbench is connected with a first inner rail telescopic rod; the right side of the bottom end of the fixed workbench is connected with a second inner rail telescopic rod; the inner middle part of the bottom end of the first lifting slip ring is rotationally connected with the first screw rod; the bottom end of the second inner rail telescopic rod is connected with the workbench supporting plate, and the left side of the top end of the workbench supporting plate is connected with the first inner rail telescopic rod; a third bearing sleeve is arranged in the middle of the bottom end of the workbench supporting plate, and the middle of the bottom end in the third bearing sleeve is connected with the first screw rod; a fourth transmission wheel is arranged in the left middle of the bottom end of the workbench supporting plate; the middle right side of the bottom end of the workbench supporting plate is rotationally connected with the first long gear; the middle part of the bottom end of the third bearing sleeve is rotationally connected with the fourth flat gear; the left side of the fourth transmission wheel is in transmission connection with the first bevel gear; the middle part of the bottom end of the fourth driving wheel is rotationally connected with the second long gear; the middle part of the bottom end of the first bevel gear is rotationally connected with the first gear carrier; the middle part of the right end of the first bevel gear is meshed with the second bevel gear, and the middle part of the left end of the second bevel gear is connected with the first gear rack; the middle part of the left end of the first long gear is meshed with the fifth flat gear; the middle part of the bottom end of the first long gear is rotationally connected with a fifth transmission wheel; the middle part of the bottom end of the fifth flat gear is rotationally connected with a third electric push rod; the middle part of the right end of the second long gear is meshed with the sixth flat gear; the middle part of the bottom end of the sixth flat gear is rotationally connected with the second electric push rod; the left side of the fifth driving wheel is in transmission connection with the sixth driving wheel; the middle part of the top end of the sixth transmission wheel is rotationally connected with the seventh flat gear; the middle part of the top end of the seventh flat gear is rotationally connected with the second gear rack; the middle part of the right end of the seventh flat gear is meshed with the eighth flat gear, the middle part of the top end of the eighth flat gear is connected with the second long gear, and the middle part of the bottom end of the eighth flat gear is connected with the second gear rack; the top end of the first electric slide rail is connected with the top curved plate; the top end of the second electric sliding rail is connected with the top curved plate; the top end of the third electric slide rail is connected with the top curved plate; the top end of the first gear rack is connected with the top curved plate; the middle part of the bottom end of the sixth transmission wheel is connected with the right bottom frame; the bottom end of the second electric push rod is connected with the right bottom frame; the bottom end of the third electric push rod is connected with the right bottom frame; the middle part of the bottom end of the fifth driving wheel is connected with the right bottom frame; the middle part of the left end of the second bevel gear is connected with the first transmission wheel.

4. The pre-treatment device for tumor cell culture bottles of claim 3, wherein the dead-corner-free rotary culture mechanism comprises a second culture bottle fixing circular plate, a universal ball, a universal bearing seat, a telescopic connecting rod, a fourth electric push rod, a fixing circular plate, a ninth flat gear, a tenth flat gear, a fifth electric push rod, an eleventh flat gear, a seventh transmission wheel, a third bevel gear, a third gear rack and a fourth bevel gear; the middle part of the bottom end of the second culture bottle fixing circular plate is rotatably connected with the universal ball; the outer surface of the universal ball is rotationally connected with the universal bearing seat; the middle part of the bottom end of the universal ball is connected with a telescopic connecting rod; the inner part of the bottom end of the telescopic connecting rod is connected with a fourth electric push rod; the bottom end of the fourth electric push rod is welded with the fixed round table plate; the middle part of the bottom end of the fixed round bedplate is rotationally connected with the ninth flat gear; a tenth flat gear is arranged at the right lower part of the ninth flat gear; the middle part of the bottom end of the tenth flat gear is rotationally connected with the fifth electric push rod; an eleventh flat gear is arranged at the right upper part of the tenth flat gear; the middle part of the top end of the eleventh flat gear is rotationally connected with a seventh transmission wheel; the right side of the seventh transmission wheel is in transmission connection with the third bevel gear; the middle part of the bottom end of the third bevel gear is rotationally connected with a third gear rack; the middle part of the right end of the third gear frame is meshed with a fourth bevel gear, and the middle part of the right end of the fourth bevel gear is connected with the third gear frame; the left end of the universal bearing seat is connected with the left bottom frame; the right end of the universal bearing seat is connected with the inner supporting plate; the right end of the third gear rack is connected with the right bottom frame; the middle part of the bottom end of the ninth flat gear is connected with the left bottom frame; the bottom end of the fifth electric push rod is connected with the left bottom frame; the middle part of the bottom end of the eleventh flat gear is connected with the left bottom frame; the upper part of the fourth bevel gear is connected with a third driving wheel.

5. The pretreatment device for tumor cell culture flasks of claim 4, wherein the first flask holder comprises a left wheel fixing plate, an inner inclined plate, a right wheel fixing plate, a fixing box, a pressing fixing plate, a second screw rod and a screwing hand wheel; the bottom of the right end of the left wheel fixing plate is connected with an inner inclined plate; the right end of the inner inclined plate is connected with the right wheel fixing plate; the middle part of the top end of the inner inclined plate is connected with the fixed box; a pressing fixing plate is arranged at the top end inside the fixing box; the inner side of the middle part of the top end of the fixed box is rotationally connected with a second screw rod, and the bottom end of the second screw rod is connected with a pressing fixed plate; the top end of the second screw rod is welded with a screwing hand wheel; the middle upper part of the left end of the left wheel fixing plate is connected with a uniform speed rotating frame wheel; the middle-lower part of the right end of the right wheel fixing plate is connected with a uniform rotating frame wheel.

6. The pretreatment device for tumor cell culture bottles of claim 5, wherein the culture solution adding mechanism comprises a ground cover, a culture solution tank, a rear fixed slide plate, a first dropping tube, a wrench switch, an angle adjusting hand wheel, a second bent dropping tube, a bottle inlet tube and a sealing edge cover; the outer surface of the grinding cover is connected with a culture solution tank; the rear side of the outer surface of the culture solution tank is connected with the rear fixed sliding plate; the middle part of the bottom end of the culture solution tank is connected with a first down liquid pipe; a spanner switch is arranged at the middle upper part of the left end of the first lower liquid pipe; the bottom of the front end of the first lower liquid pipe is connected with an angle adjusting hand wheel; the bottom end of the first liquid descending pipe is connected with the second bent liquid descending pipe, and the left side of the front end of the second bent liquid descending pipe is connected with the angle adjusting hand wheel; the middle part of the right end of the second bending liquid dropping pipe is connected with the bottle inlet pipe; the middle part of the outer surface of the bottle inlet pipe is connected with the sealing edge cover; the middle part of the rear end of the rear fixed sliding plate is connected with a second electric sliding rail.

7. The pre-treatment device for tumor cell culture bottles of claim 6, wherein the first and second culture bottle holders are at thirty degrees and three hundred thirty degrees respectively from the positive direction of the horizontal X-axis.

8. The pre-treatment device for tumor cell culture flasks of claim 7, wherein the left end and the right end of the fixed worktable are respectively provided with an arc-shaped groove plate.