CN110962902B - Cell factory culture transfer trolley and cell factory transfer method - Google Patents
Cell factory culture transfer trolley and cell factory transfer method Download PDFInfo
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- CN110962902B CN110962902B CN201911304017.5A CN201911304017A CN110962902B CN 110962902 B CN110962902 B CN 110962902B CN 201911304017 A CN201911304017 A CN 201911304017A CN 110962902 B CN110962902 B CN 110962902B
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- 238000012546 transfer Methods 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000003825 pressing Methods 0.000 claims abstract description 81
- 230000000670 limiting effect Effects 0.000 claims description 36
- 230000001681 protective effect Effects 0.000 claims description 14
- 230000007306 turnover Effects 0.000 claims description 9
- 238000004113 cell culture Methods 0.000 abstract description 11
- 210000004027 cell Anatomy 0.000 description 161
- 230000009471 action Effects 0.000 description 8
- 238000003780 insertion Methods 0.000 description 7
- 230000037431 insertion Effects 0.000 description 7
- 238000003860 storage Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 238000005096 rolling process Methods 0.000 description 5
- 238000006073 displacement reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000002349 favourable effect Effects 0.000 description 4
- 238000009434 installation Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 210000001503 joint Anatomy 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000012007 large scale cell culture Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 210000000707 wrist Anatomy 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
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- 238000011105 stabilization Methods 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 210000001912 transporting cell Anatomy 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B62—LAND VEHICLES FOR TRAVELLING OTHERWISE THAN ON RAILS
- B62B—HAND-PROPELLED VEHICLES, e.g. HAND CARTS OR PERAMBULATORS; SLEDGES
- B62B3/00—Hand carts having more than one axis carrying transport wheels; Steering devices therefor; Equipment therefor
- B62B3/04—Hand carts having more than one axis carrying transport wheels; Steering devices therefor; Equipment therefor involving means for grappling or securing in place objects to be carried; Loading or unloading equipment
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B62—LAND VEHICLES FOR TRAVELLING OTHERWISE THAN ON RAILS
- B62B—HAND-PROPELLED VEHICLES, e.g. HAND CARTS OR PERAMBULATORS; SLEDGES
- B62B3/00—Hand carts having more than one axis carrying transport wheels; Steering devices therefor; Equipment therefor
- B62B3/002—Hand carts having more than one axis carrying transport wheels; Steering devices therefor; Equipment therefor characterised by a rectangular shape, involving sidewalls or racks
- B62B3/005—Details of storage means, e.g. drawers, bins or racks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B62—LAND VEHICLES FOR TRAVELLING OTHERWISE THAN ON RAILS
- B62B—HAND-PROPELLED VEHICLES, e.g. HAND CARTS OR PERAMBULATORS; SLEDGES
- B62B3/00—Hand carts having more than one axis carrying transport wheels; Steering devices therefor; Equipment therefor
- B62B3/02—Hand carts having more than one axis carrying transport wheels; Steering devices therefor; Equipment therefor involving parts being adjustable, collapsible, attachable, detachable or convertible
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B62—LAND VEHICLES FOR TRAVELLING OTHERWISE THAN ON RAILS
- B62B—HAND-PROPELLED VEHICLES, e.g. HAND CARTS OR PERAMBULATORS; SLEDGES
- B62B5/00—Accessories or details specially adapted for hand carts
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Combustion & Propulsion (AREA)
- Transportation (AREA)
- Mechanical Engineering (AREA)
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Abstract
The invention discloses a cell factory culture transfer trolley and a cell factory transfer method, and relates to the technical field of cell culture transfer. The movable saddle is in split sliding fit with the main body frame, and a positioning device for locking the movable saddle and the main body frame is arranged on the movable saddle; and the pressing component comprises a pressing plate for pressing down and fixing the cell factory, and a locking device for locking the pressing state of the pressing plate is connected between the pressing plate and the main body frame. The portable saddle cooperatees with main body frame and realizes the transportation operation and the transfer operation of cell factory to cooperate through pushing down subassembly and main body frame and realize the transportation of cell factory fixed, and then reach the purpose that improves cell factory and transport operation flexibility and convenience.
Description
Technical Field
The invention relates to the technical field of cell culture transfer, in particular to a cell factory culture transfer trolley and a cell factory transfer method.
Background
Cell culture (cellculture), also known as cell culture technology, refers to a method for simulating in vivo environment (sterility, suitable temperature, pH value, certain nutritional conditions, etc.) in vitro to allow cells to survive, grow, reproduce and maintain their main structure and function.
When a small amount of cell culture is carried out in a laboratory, the cell culture is generally finished by adopting culture bottles, culture dishes and the like; when a large amount of cells are cultured in a laboratory, a multi-layer culture bottle or a 1-5-layer cell factory can be selected to complete the cell culture; when large-scale cell culture is carried out, a cell culture reactor, a spinner flask or a multi-layer cell factory is required to be selected for completing the cell culture. The cell culture reactor is expensive and has high purchasing cost, and the use of the spinner flask for culturing cells occupies large space, the difference between flasks is difficult to control, and the potential danger of DNA toxicity exists. Therefore, the cell factory (adopting the low-temperature ultra-vacuum plasma cell adherent surface treatment technology for treatment) becomes a preferred mode for large-scale cell culture and production due to the advantages of compact structure, space saving, no cytotoxicity and convenience for the number of layers to be amplified.
The prior Chinese patent with the publication number of CN2017162075U discloses a cell factory frame, which comprises a frame main body, wherein a plurality of groups of vertical cavities arranged side by side are arranged in the frame main body, and a pressing plate used for pressing and fixing each cell factory is arranged above a placing opening at the upper end of each vertical cavity. The side of frame main part is provided with the location and snatchs the dish, and the location snatchs the dish including snatching a dish main part, and the middle part of snatching a dish main part is provided with snatchs the spliced eye, and is provided with annular joint recess on the inside wall of snatching the spliced eye, is provided with the locating hole on the outside contact surface of snatching a dish main part, then the frame tongs of its robotic wrist front end of multiaxis robotic arm accessible and snatch a dish main part and realize snatching the cooperation. Put the cell factory in cell factory frame, and cell factory frame and multiaxis robotic arm cooperate and realize rocking, operation such as rotation, are favorable to realizing the high-efficient production of scale.
When transporting cell factory, at first put into each cell factory to cell factory frame internal fixation (fixed through the pressure strip), will be equipped with the cell factory frame of cell factory again and place on the culture rack in the constant temperature chamber, then, multiaxis robotic arm utilizes the frame tongs that front end manipulator wrist department set up to be connected with the cooperation of location grabbing dish, snatchs the cell factory frame from culture rack and removes to corresponding operation point position department to accomplish the bulk movement of cell factory with cell factory frame. However, in the above prior art, the cell factory frame is a fixed frame structure, which is difficult to be manually transferred, and the working coverage of the multi-axis robot is limited, i.e. the multi-axis robot and the cell factory frame are matched to only realize cell factory transfer in the same thermostatic chamber, but not transfer between different thermostatic chambers or between working chambers; meanwhile, when an operator places the cell factory in the cell factory frame or takes out the cell factory frame, the pressure plate needs to be integrally installed or taken out, which causes great inconvenience for the operator to take or place the cell factory. Therefore, how to increase the flexibility of transportation and the convenience of transportation of cell factories becomes a problem to be solved, and the prior art has a point to be improved.
Disclosure of Invention
In view of the above technical problems, an object of the present invention is to provide a cell factory culture transfer cart, which realizes the transfer operation of a cell factory by the cooperation of a movable cart seat and a main body frame, and realizes the transfer fixation of the cell factory by the cooperation of a pressing component and the main body frame, thereby achieving the purpose of improving the flexibility and convenience of the transfer operation of the cell factory.
In order to achieve the purpose, the invention provides the following technical scheme: a cell factory culture transfer trolley comprises a main body frame used for storing and transferring a cell factory, wherein a movable type vehicle seat matched with the main body frame is arranged at the bottom of the main body frame, and a pressing component used for pressing and locking the cell factory is arranged at the top of the main body frame; the movable saddle is provided with a split moving track, the main body frame is in sliding fit with the split moving track, and the movable saddle is also provided with a positioning device for fixing the position of the main body frame; the positioning device comprises a positioning shaft and an elastic piece for driving the positioning shaft to extend out of the movable saddle, the positioning shaft is matched with the movable saddle in a telescopic sliding mode and is in splicing fit with the main body frame, and the elastic piece is arranged in the movable saddle in a telescopic mode; the lower pressing assembly comprises a lower pressing plate positioned above a vertical opening of the main body frame, the lower pressing plate and the main body frame are connected with a locking device, the locking device comprises an overturning connecting plate arranged on the main body frame, the overturning connecting plate is connected with a pull buckle extending plate, the pull buckle extending plate and the lower pressing plate are connected with a pull buckle, a hook block is arranged on the pull buckle extending plate, and the hook block is in hook joint with the main body frame; the turnover connecting plate is in running fit with the extension plate of the pull buckle, the extension plate of the pull buckle is in sliding fit with the turnover connecting plate at the same time, and the lower pressing plate is turned over towards any one side of the main body frame under the guide of the running sliding fit of the turnover connecting plate and the extension plate of the pull buckle and is lapped on the outer side of the main body frame.
Through adopting above-mentioned technical scheme, when needing the storage to transport cell factory, the user places cell factory in main body frame, then with holding down plate upset to main body frame's top and push down in the top of cell factory to under the state that the tab is untied, accomplish the hook piece of tab extension plate and the cooperation of colluding of main body frame, the tab of locking holding down plate again, thereby reach the purpose of pushing down fixed cell factory. When the cell factory in the main body frame is taken to needs, operating personnel unties locking device's the knot that draws to the downward separation colludes piece and catching groove, then rotate towards one side of main body frame and draw the knot extension plate, draw the knot extension plate upwards to lift simultaneously, promptly at the upset in-process, the holding down plate can not take place the structure with the cell factory and interfere, and draw after the upset and detain the extension plate and can overlap joint in the outside of main body frame, and need not with main body frame overall separation, be convenient for the operating personnel and place or take the cell factory. When the main body frame is combined with the movable saddle and the positioning device locks the main body frame and the movable saddle, an operator can stably transfer the cell factory between different thermostatic chambers or between a working chamber and the thermostatic chambers. When the locking state of the positioning device is released and the main body frame and the movable vehicle seat are separated in a sliding manner, an operator can integrally transfer the cell factory and the main body frame into the thermostatic chamber, and meanwhile, the main body frame in which the cell factory is stored in the thermostatic chamber can be integrally and smoothly transferred onto the movable vehicle seat, so that the positioning device has good transfer convenience. The portable saddle cooperatees the transportation operation that realizes the cell factory with main body frame to cooperate the transportation that realizes the cell factory through pushing down the subassembly and main body frame and fix, and then reach the purpose that improves cell factory and transport operation flexibility and convenience.
The invention is further configured to: a limiting stop block is formed on the positioning shaft, a limiting baffle is detachably mounted at the bottom of the movable saddle, and a limiting groove is formed in the limiting baffle; the elastic piece is clamped between the limit stop and the limit baffle, and two ends of the elastic piece are respectively abutted against the limit stop and the limit groove; the positioning shaft is connected with a pedal plate, and the pedal plate extends out of the movable vehicle seat.
Through adopting above-mentioned technical scheme, adopt mode installation elastic component that centre gripping butt, coaxial cover were established to realize that the elastic component drive location axle stretches out the purpose of locking main part frame, simultaneously, both be convenient for assembly personnel installation, debugging elastic component, be convenient for again follow-up maintenance, change elastic component. The pedal is stepped on by the foot of an operator to achieve the purpose of overcoming the elastic driving force of the elastic piece to reversely drive the positioning shaft to move, namely, the purpose of driving the positioning shaft to be separated from the main body frame is achieved, so that the main body frame and the movable saddle can be conveniently separated, and the pedal has better practicability and use convenience.
The invention is further configured to: the positioning shaft is connected with a driving shaft which is arranged in parallel with the positioning shaft, and the driving shaft and the positioning shaft synchronously move and are in sliding fit with the movable saddle; the footboard is installed on the driving shaft, and the footboard extends to the outside of portable saddle from the bottom of portable saddle.
Through adopting above-mentioned technical scheme, adopt location axle and drive shaft matched with biax drive structure to replace the unipolar drive structure that only uses the location axle originally, be favorable to improving the job stabilization nature and the life of location axle. The pedal effort is at first concentrated on the drive shaft, transmits to the location epaxially through the drive shaft again, and location axle atress is more steady, even, and the elastic component installs on the location is epaxial, maintains to change the drive shaft and compare in maintaining to change the location axle more simple and convenient, when the drive shaft leads to taking place to damage because of the atress problem promptly, the maintainer of being convenient for overhauls, changes. Meanwhile, on the basis, the driving shaft is introduced, so that the height of lifting the feet of an operator can be reduced, and the foot lifting device has better practicability and use convenience.
The invention is further configured to: the movable vehicle seat is connected with a protective baffle, the pedal penetrates through the protective baffle, and the protective baffle is provided with a notch for the movement of the pedal; the protective baffle is provided with an inserting column, when the pedal plate is in a pressing state, a limiting block is clamped between the inserting column and the pedal plate and matched with the inserting column in an inserting mode.
By adopting the technical scheme, when an operator lifts a foot and treads the pedal plate, the foot is easy to directly contact with the driving shaft, and the driving shaft is easy to damage or unsmooth work when the foot is collided for a long time or violently collided; therefore, the protective baffle plays a role in shielding and protecting the driving shaft, and meanwhile, the opening of the notch does not influence the normal treading of the pedal plate by an operator and the normal movement of the pedal plate. When the separation state of the main body frame and the movable saddle needs to be kept for a long time, an operator cannot always keep the state of treading the pedal plate, and under the condition, the rebounding motion of the pedal plate can be limited by using a mode that the limiting block is matched with the inserting block, namely the aim of conveniently and stably releasing the locking state of the positioning device is fulfilled.
The invention is further configured to: the split moving track comprises rollers which are rotatably arranged at the top of the movable saddle, guide blocks are arranged among the rollers at intervals, a guide groove is formed in a bottom plate of the main body frame corresponding to the guide blocks, and the guide blocks are in butt joint with the guide groove and are in sliding fit with the guide groove.
By adopting the technical scheme, the main body frame and the movable saddle are separated in a sliding mode through the roller, and in the process that the main body frame is combined with or separated from the movable saddle, the guide block is matched with the guide groove to achieve the purpose of stably and smoothly guiding the main body frame to move, namely the main body frame is more stable in sliding movement along the split moving track, and the main body frame is more accurately butted with the split moving track.
The invention is further configured to: the two ends of the main body frame in the length direction are respectively provided with a first handle, and one side of the movable vehicle seat in the width direction of the main body frame is provided with a second handle; the side plate of the movable vehicle seat is provided with an inserting block, and the second handle is matched with the inserting block in an inserting mode.
By adopting the technical scheme, an operator can transfer the movable vehicle seat and the main body frame in different directions by utilizing the first handle and the second handle, so that the movable vehicle seat has better practicability and use convenience, and can adapt to working conditions of different environments; and the second handle is pegged graft with the grafting piece and is cooperated, and the second handle can be dismantled with portable saddle and be connected promptly, and operating personnel can be according to the actual conditions of transportation environment, whether the selection is installed, is used the second handle, has better use flexibility and suitability.
The invention is further configured to: the main body frame comprises a movable saddle, a support frame is arranged on the movable saddle, the support frame comprises outer support frames positioned at two ends of the movable saddle in the length direction, middle support frames are arranged between the two outer support frames at equal intervals, and the outer support frames and the middle support frames are connected with side baffles extending along the length direction of the movable saddle; the side baffle comprises two support plates, the two support plates are respectively in running fit with the corresponding outer side support frames, and the support plates and the middle support frame are connected with locking pieces.
Through adopting above-mentioned technical scheme, adopt two extension boards cooperation to constitute the structure of side shield, replace the mounting structure of original whole root side shield. Because in original whole root side shield mounting structure, main stress point is at the both sides tip of side shield, and in two extension board cooperations constitute the side shield structure, main stress point is in the inferior central point department of putting of side shield, and the stress size still satisfies the use needs, and on this basis, operating personnel can place or take the cell factory from main body frame's side direction, is favorable to improving operating personnel's operation convenience promptly.
The invention is further configured to: the turnover connecting plate is provided with a limiting piece for limiting the turnover position of the lower pressing plate, the extension plate of the pull buckle is provided with a notch, and the limiting piece is in clamping fit with the notch; when the extension plate of the pull buckle is turned and clamped with the limiting part, the turning connecting plate is embedded with the opening groove of the hook block to be in butt fit.
Through adopting above-mentioned technical scheme, when operating personnel need unblock and upset holding down plate, the locating part on the upset connecting plate plays the restriction and draws the pivoted effect of detaining extension plate, promptly when upset holding down plate to one side, the holding down plate no longer overlaps on main body frame, but realizes hovering fixed purpose through the joint cooperation of locating part and opening, meanwhile, the upset connecting plate inlays with the open groove of colluding the piece and establishes the butt cooperation to reach the purpose of further restriction holding down plate upset. The locating part cooperates with the joint of the opening, and the overturning connecting plate cooperates with the embedding of the hook block, so that the operator can overturn the fixed lower pressing plate conveniently, and the main body frame and the cell factory can be protected.
In view of the above technical problems, an object of the present invention is to provide a cell factory transferring method, which uses a movable vehicle seat in cooperation with a main body frame to implement transferring operations or transferring operations under different working environments and working requirements, thereby achieving the purpose of improving flexibility and convenience of transferring or transferring operations of a cell factory.
In order to achieve the purpose, the invention provides the following technical scheme: a cell factory transfer method comprises the transfer operation of a cell factory culture transfer trolley, the transfer operation of a main body frame storing a cell factory and the filling and unloading operation of the cell factory, and specifically comprises the following steps of S1, filling the cell factory into the main body frame of the cell factory culture transfer trolley, and fixing the cell factory through a pressing component; s2-1, transferring a cell factory culture transfer trolley filled with a cell factory between adjacent thermostatic chambers or between a working chamber and the thermostatic chambers; after the culture is finished, the cell factory culture transfer trolley is transferred out of the thermostatic chamber, the pressing component is unlocked, the support plate is turned over, and the cell factory on the main body frame is unloaded; s2-2, transferring the cell factory to a constant temperature box by using a cell factory culture transfer trolley, unlocking a positioning device, and transferring a main body frame filled with the cell factory into the constant temperature box; after the culture is finished, transferring the main body frame filled with the cell factory in the incubator to a movable vehicle seat, locking the main body frame through a positioning device, transferring the cell factory culture transfer vehicle to a specified position, unlocking a pressing component, overturning a support plate, and unloading the cell factory on the main body frame; s2-3, transferring the cell factory to a thermostat or a thermostatic chamber by using a cell factory culture transfer trolley, unlocking the pressing component and turning over the support plate, and unloading the cell factory on the main body frame to the thermostat or the thermostatic chamber; and after the culture is finished, the cell factory is refilled on the main body frame, the cell factory is transported to a specified position by using the cell factory culture transfer trolley, the pressing component is unlocked, the support plate is turned over, and the cell factory on the main body frame is unloaded.
By adopting the technical scheme, aiming at different transfer operation requirements or transfer operation requirements, an operator can select according to actual conditions, the cell factory culture transfer vehicle formed by the movable vehicle seat and the main body frame can be used for completing the transfer operation of a cell factory between thermostatic chambers or between the thermostatic chambers and the working chambers, and the transfer operation of the cell factory between the thermostatic chambers and the transfer operation between the thermostatic chambers (or the working chambers) and the thermostatic boxes can be realized through the split sliding fit of the movable vehicle seat and the main body frame, so that the cell factory culture transfer vehicle has good use flexibility and adaptability.
The invention is further configured to: a guide rail matched with the movable vehicle seat is arranged in the thermostat in the S2-2, the guide rail comprises a rail frame and roll shafts rotatably arranged on the rail frame, guide blocks are arranged between the roll shafts at equal intervals, and the guide blocks are in butt joint and sliding fit with the guide grooves of the main body frame.
Through adopting above-mentioned technical scheme, when main body frame cultivateed the transfer car by the cell factory and inwards shift to the thermostated chamber, main body frame's guide way can with the guide block butt cooperation on the guide track, play the effect that guide main body frame is smoothly, stably shifted to in the thermostated container by portable saddle.
In conclusion, the invention has the following beneficial effects:
one is as follows: the movable vehicle seat is matched with the main body frame to realize the transferring operation of the cell factory, and the pressing assembly is matched with the main body frame to realize the transferring fixation of the cell factory, so that the purposes of improving the flexibility and convenience of the transferring operation of the cell factory are achieved;
the second step is as follows: the lower pressing plate is matched with the locking device to press and lock the cell factory, and meanwhile, the lower pressing plate can be suspended and clamped on the overturning connecting plate without being integrally separated from the main body frame and without structural interference with the cell factory or the main body frame, so that the cell factory locking device has better use convenience and pressing stability;
and thirdly: the locking and matching of the main body frame and the movable saddle are realized by adopting a double-shaft structure formed by matching the driving shaft and the positioning shaft, the split sliding and matching of the main body frame and the movable saddle are not influenced, and the driving stability and the use safety are better;
fourthly, the method comprises the following steps: the side baffle plate of the main body frame is composed of two support plates, and an operator can place a cell factory or take the cell factory in the main body frame by turning over the corresponding support plate, so that the stress requirement of the main body frame is met, and the use convenience of the operator is improved;
and fifthly: aiming at different transfer operation requirements or transfer operation requirements, an operator can select the operation according to actual conditions, and the operation method has good use flexibility and adaptability.
Drawings
FIG. 1 is a schematic view showing the general structure of a cell factory culture transfer cart according to a first embodiment;
FIG. 2 is a schematic structural view of a main body frame;
FIG. 3 is a schematic view of the outer side of the locking device;
FIG. 4 is a schematic view of the inner side of the locking device;
FIG. 5 is a partial schematic view of a body frame primarily used to show a hook groove;
FIG. 6 is a schematic view mainly used for showing the structure of the hold-down groove;
FIG. 7 is a schematic view showing the overall structure of a cell factory culture transfer cart in the second embodiment;
fig. 8 is a schematic structural view mainly showing a movable vehicle seat;
FIG. 9 is a schematic structural view mainly used for showing a main body frame;
FIG. 10 is a schematic view of the structure of the drum;
FIG. 11 is a schematic view mainly illustrating a connecting structure of the positioning device and the seat main body;
FIG. 12 is a schematic view of the positioning device;
FIG. 13 is a schematic view showing the structure of the brackets of the cell factory culture transfer trolley according to the third embodiment;
FIG. 14 is a block diagram showing a flow of a cell factory transferring method according to the fourth embodiment;
FIG. 15 is a schematic view of a transfer structure mainly used for showing a thermostatic chamber and a cell factory culture transfer vehicle;
fig. 16 is a schematic view of the structure of the guide rail.
Reference numerals: 1. a main body frame; 11. a storage space; 12. a taking and placing port; 13. a first handle; 14. a second handle; 15. a bottom frame; 151. inserting positioning holes; 16. a guide groove; 17. a cell factory; 2. a movable vehicle seat; 21. a roller; 22. an insertion block; 23. a seat main body; 24. a split moving track; 25. a rolling groove; 251. mounting grooves; 252. a guide block; 26. a drum; 261. a barrel; 262. a roller; 3. a support frame; 31. an outer support frame; 311. hooking a groove; 312. a top horizontal plate member; 32. a middle support frame; 33. a side dam; 34. a support plate; 341. embedding a groove; 342. a locking member; 35. a step structure; 4. a lower pressing plate; 41. pressing the groove downwards; 42. elastic pressing blocks; 5. a locking device; 51. turning over the connecting plate; 511. an ear plate; 512. a connecting member; 513. a limiting member; 52. a tab extension plate; 521. a main board; 522. a side plate; 523. connecting grooves; 524. opening the gap; 53. pulling the button; 531. a locking end; 532. clamping the fixed end; 533. clamping the shaft; 54. hooking the block; 541. a guide ramp; 542. opening the groove; 55. pulling and pressing a buckle; 551. an elastic clamping groove; 6. a positioning device; 61. positioning the shaft; 611. a limit stop block; 62. an elastic member; 63. an upper shaft sleeve of the pedal; 64. a drive shaft; 65. a connecting plate; 66. a lower shaft sleeve of the pedal; 67. a foot pedal; 7. a limit baffle; 71. a limiting groove; 8. a protective baffle; 81. a channel; 82. inserting the column; 9. a guide rail; 91. a rail frame; 92. a roll shaft; 93. and a guide block.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
The first embodiment is as follows:
as shown in fig. 1, a push-down cell factory culture transfer vehicle comprises a main body frame 1, wherein the main body frame 1 comprises a movable saddle 2 and a support frame 3 fixed on the movable saddle 2, the movable saddle 2 is a square structure, the support frame 3 comprises two outer support frames 31 positioned at two ends of the movable saddle 2 in the length direction, a middle support frame 32 is arranged between the two outer support frames 31 at equal intervals along the length direction of the movable saddle 2, two side edges of the outer support frames 31 and the middle support frame 32 (two side edges of the movable saddle 2 in the width direction) are connected with side baffles 33 in an extending manner along the length direction, and the side baffles 33 play a role of fixing a cell factory 17. As shown in FIGS. 1 and 2, a storage space 11 for storing and transporting the cell factory 17 is formed between adjacent intermediate scaffolds 32 or between the intermediate scaffold 32 and the outer scaffold 31. The cell factory 17 is stored in the storage space 11 of the main body frame 1, and an operator can move the main body frame 1 to complete the transportation operation of the cell factory 17.
As shown in fig. 2, a plurality of access openings 12 are formed at the upper end of the main body frame 1, that is, the top ends of the adjacent middle support frames 32 or the top ends of the middle support frames 32 and the top ends of the outer support frames 31 form an opening structure. A lower pressing plate 4 is further arranged above the taking and placing opening 12 of the main body frame 1, the left end and the right end of the lower pressing plate 4 are connected with the main body frame 1 through locking devices 5, and the lower pressing plate 4 is matched with the locking devices 5 to realize downward pressing type locking storage of the cell factory 17, namely, the cell factory 17 is stored in the storage space 11 through the taking and placing opening 12 and is fixed in the main body frame 1 under the downward pressing effect of the lower pressing plate 4.
Referring to fig. 2 and 3, the locking device 5 includes a turning connecting plate 51 which is locked and installed on the outer support frame 31 by screws, a pulling buckle extending plate 52 is connected between the turning connecting plate 51 and the lower press plate 4, a pulling buckle 53 is connected to the upper end of the pulling buckle extending plate 52 and the end corresponding to the lower press plate 4, the lower end of the pulling buckle extending plate 52 and the outer support frame 31 form a hooking and matching structure, and the side edge of the pulling buckle extending plate 52 is in sliding and rotating fit with the turning connecting plate 51. The lower press plate 4 realizes the reducing and turning movement through the matching of the turning connecting plate 51 and the pull buckle extension plate 52, and realizes the pressing-down type locking function through the hooking matching structure of the pull buckle 53 and the main body frame 1.
When the cell factory 17 needs to be locked by pressing down, the operator pulls up and turns over the lower pressing plate 4 through the tab extension plate 52, so that the lower pressing plate 4 turns over right above the cell factory 17, and then releases the tab extension plate 52 downward, so that the lower end of the tab extension plate 52 is hooked with the outer support frame 31, and finally the locking operation of the tab 53 is completed (in the process, the tab extension plate 52 has upward slight displacement motion), so that the locking operation of the lower pressing plate 4 is completed. When the press-down locking of the lower press plate 4 needs to be released, the operator first releases the locking of the tab 53, then releases the hooking engagement of the tab extension plate 52 and the outer support frame 31 (in this process, the tab extension plate 52 has a slight downward displacement movement), and then turns over the lower press plate 4 toward one side of the main body frame 1, and in this process, it is necessary to appropriately lift the tab extension plate 52 upward and simultaneously rotate the tab extension plate 52, that is, in the process of turning over the lower press plate 4, the lower press plate 4 does not structurally interfere with the cell factory 17, and the unlocking operation of the lower press plate 4 is completed.
Referring to fig. 2 and 3, the turnover connection plate 51 is vertically installed on the outer side of the top horizontal plate 312 of the outer support frame 31, referring to fig. 3 and 4, an ear plate 511 is formed by extending the turnover connection plate 51 near the vertical side edge of the tab extension plate 52, a connection member 512 for realizing the rotation sliding fit with the tab extension plate 52 is installed and fixed on the ear plate 511, and a screw can be used as the connection member 512. Draw and detain extension plate 52 including with lower clamp plate 4, the mainboard 521 that outside support frame 31 cooperation was used, one side extension that mainboard 521 is close to upset connecting plate 51 is formed with curb plate 522, and extend along its length direction on the curb plate 522 and set up and be formed with the spread groove 523, then the screw is worn to establish the spread groove 523 and is slided the cooperation with the spread groove 523, curb plate 522 passes through the spread groove 523 and the connecting piece 512 realizes the normal running fit with upset connecting plate 51, operating personnel both can rotate and draw and detain extension plate 52, can follow the spread groove 523 again and slide the removal and draw and detain extension plate 52.
Referring to fig. 3 and 4, a screw hole is formed in the turning connection plate 51, a limiting member 513 for limiting the turning position of the extension plate 52 of the buckle is installed in the screw hole, and a screw can also be used as the limiting member 513. The side of curb plate 522 is kept away from mainboard 521 is formed with opening 524, when operating personnel carried upset holding down plate 4 to the position department of hovering, opening 524 and the locating part 513 joint cooperation of curb plate 522, and the extension plate 52 of drawing after the upset is in the horizontal state of hovering, and at this moment, holding down plate 4 just rotates to one side of main body frame 1 and keeps vertical state (at this moment, holding down plate 4 is located its high intermediate position department on the direction of height of main body frame 1), holding down plate 4 does not contact with cell factory 17 and main body frame 1 promptly, the operating personnel of both being convenient for overturn holding down plate 4, also be convenient for operating personnel place or take cell factory 17.
As shown in fig. 4 and 5, the bottom end of the tab extension plate 52 is detachably mounted with a hook block 54 that is hooked and matched with the outer side support frame 31 for use, the hook block 54 is connected with the tab extension plate 52 through a screw, the hook block 54 is matched with the inner side surface of the tab extension plate 52 to form an open groove 542, and meanwhile, the bottom end of the top horizontal plate 312 of the outer side support frame 31 is formed with a hook groove 311, so that the hook block 54 is hooked and matched with the hook groove 311, thereby realizing the hooking and fixing of the tab extension plate 52 and the outer side support frame 31.
As shown in fig. 4, since the hook 54 interferes with the protrusion structure of the top horizontal plate 312 of the outer bracket 31 for forming the hook 311 during the hooking engagement of the hook 54 with the hook 311, the hook 54 is made of an elastic material, and a guiding inclined plane 541 is formed at the end of the hook 54, so as to facilitate the engagement and disengagement of the hook 54 and the hook 311. In order to further improve the suspension stability of the lower pressing plate 4, when the tab extension plate 52 is turned over and is engaged with the limiting member 513, the side edge of the turning connection plate 51 is embedded and abutted in the opening groove 542 of the hook block 54.
Referring to fig. 3 and 4, the tab 53 generally includes a spring tab, a fixed tab, an adjustable buckle, and the like, and in this embodiment, a spring tab (a spring of the spring tab is not shown in the drawings) is used; the locking end 531 of the spring tab is fixed to the end of the lower platen 4, and the fastening end 532 of the spring tab is fixed to the end of the tab extension 52 near the lower platen 4. The end of the extension plate 52 close to the lower plate 4 is fixed with a pull-buckle pressing plate 55, and an elastic clamping groove 551 is formed on the pull-buckle pressing plate 55, so that the clamping shaft 533 of the clamping fixed end 532 is elastically clamped and fixed with the elastic clamping groove 551.
Referring to fig. 1 and 6, after the lower pressing plate 4 is turned to the down-pressing locking position, the lower pressing plate 4 is located at the middle position in the width direction of the main frame 1 and is parallel to the length direction of the main frame 1, that is, the stability of the cell factory 17 in storage and transportation is improved by limiting the down-pressing locking position of the lower pressing plate 4. A lower pressing groove 41 is formed on the lower pressing plate 4 facing the bottom of the main body frame 1, and an elastic pressing block 42 is embedded and fixed in the lower pressing groove 41; when the lower pressing plate 4 presses down and fixes the cell factory 17, the elastic pressing block 42 presses down and abuts on the top of the cell factory 17, i.e. the elastic pressing block 42 plays a role of pressing down and fixing the cell factory 17 as well as a role of protecting the cell factory 17.
As shown in FIG. 2, in order to facilitate the transportation of the cell factory culture transport cart by the user, first handles 13 are provided at both ends of the main body frame 1 in the longitudinal direction, i.e., the first handles 13 are respectively connected to the top horizontal plate members of the outer support frames 31. Two inserting blocks 22 are fixedly arranged at any one side edge of the movable vehicle seat 2 in the width direction, and a second handle 14 is arranged on the two inserting blocks 22 in a matched inserting manner; the operator can choose to use the first handle 13 or the second handle 14, or even choose to install the second handle 14 or to remove the second handle 14, depending on the situation.
The present embodiment is further described below with reference to specific actions:
when the cell factory 17 is stored in the main body frame 1 and the lower press plate 4 is turned to one side of the main body frame 1, the operator needs to turn the lower press plate 4 to the top of the main body frame 1 again, that is, the turning action is completed by the rotation matching of the connecting piece 512 on the turning connecting plate 51 and the connecting groove 523 on the tab extension plate 52, and in the process, the connecting piece 512 and the connecting groove 523 slide, are in insertion matching to realize the displacement motion of the tab extension plate 52. After the lower pressing plate 4 is turned over to the top position of the main body frame 1 (at this time, the lower pressing plate 4 should be located at the middle position in the width direction of the main body frame 1), the pulling buckle extension plate 52 is pulled vertically and downwardly by matching the connecting piece 512 with the connecting groove 523 until the hook block 54 at the bottom end of the pulling buckle extension plate 52 is hooked and matched with the corresponding hook groove 311 on the outer support frame 31 (at this time, the pulling buckle 53 connected with the lower pressing plate 4 at the upper end of the pulling buckle extension plate 52 is in an unlocked state), and finally, the locking action of the pulling buckle 53 is completed, so that the lower pressing plate 4 is tightly pressed on the top of the cell factory 17, thereby completing the pressing and locking operation of the cell factory 17. When the cell factory 17 in the main body frame 1 needs to be taken out, the operator completes the above operations in a reverse direction, that is, firstly, the locking of the tab 53 is released, meanwhile, the hooking of the hook block 54 and the hook groove 311 is released, and the turning displacement action of the turning connecting plate 51 and the tab extending plate 52 is realized by the matching of the connecting piece 512 and the connecting groove 523, so that the lower pressing plate 4 is turned to one side of the main body frame 1, at this time, the notch 524 on the side plate 522 of the tab extending plate 52 is clamped with the limiting piece 513 on the turning connecting plate 51, and the side edge of the turning connecting plate 51 is just embedded into the opening groove 542 abutted against the hook block 54, so that the lower pressing plate 4 is located at the middle position in the height direction of the main body frame 1, thereby completing the taking out operation for unlocking the cell factory 17.
Example two:
as shown in FIG. 7, a cell factory culture transfer cart comprises a movable saddle 2 and a main body frame 1 mounted on the movable saddle 2, a cell factory 17 is stored in the main body frame 1, and an operator can transfer the cell factory 17 between different thermostatic chambers or between a working chamber and a thermostatic chamber through the movable saddle 2. As shown in fig. 7 and 8, the movable seat 2 includes a seat main body 23 and a roller 21 mounted at the bottom of the seat main body 23, and a split moving rail 24 slidably engaged with the bottom of the main frame 1 is formed at the top of the seat main body 23; the operator can slide and separate the main body frame 1 and the movable vehicle seat 2, i.e., the cell factory 17 can be transferred from the movable vehicle seat 2 to the constant temperature test chamber. Main body frame 1 cooperatees with portable saddle 2, both can realize cell factory 17's transportation operation, can realize cell factory 17's transfer operation again, has better use flexibility and environmental condition adaptability.
As shown in fig. 8, the seat main body 23 is a square structure, the top of the seat main body 23 is formed with a rolling groove 25, the rolling groove 25 extends along the length direction of the seat main body 23 and penetrates through the seat main body, a plurality of mounting grooves 251 are formed on two side walls of the rolling groove 25 at equal intervals, and the rollers 26 are rotatably mounted in the mounting grooves 251 correspondingly formed on the two sides, that is, the plurality of rollers 26 together form a roller row. As shown in fig. 8 and 9, the bottom of the main body frame 1 is fixedly connected with a bottom frame 15 used in cooperation with the split moving rail 24, and the bottom surface of the bottom frame 15 is in sliding, abutting and matching with the upper cylindrical surface of the roller row, so as to realize the split sliding and matching of the main body frame 1 and the movable vehicle seat 2. The rolling grooves 25 are internally provided with guide blocks 252 at equal intervals, namely the guide blocks 252 are arranged in the gap between the adjacent rollers 26 at equal intervals, and the bottom plate of the bottom frame 15 is provided with guide grooves 16 along the length direction; the width of the guiding block 252 is matched with the width of the guiding groove 16, and the guiding block 252 and the guiding groove 16 are correspondingly arranged, so that the bottom frame 15 can realize smooth and stable sliding movement of the main body frame 1 and the movable saddle 2 by the sliding abutting fit of the guiding groove 16 and the guiding block 252, and simultaneously, the butt joint accuracy of the main body frame 1 and the movable saddle 2 is improved. The split moving rail 24 is formed by matching a roller 26 row consisting of rollers 26 with the guide block 252, and the main body frame 1 is in sliding fit with the split moving rail 24, so that the transfer operation with the movable vehicle seat 2 is realized.
As shown in fig. 8 and 10, the roller 26 includes a cylindrical body 261, a roller 262 coaxially penetrates and is fixed in the cylindrical body 261, two ends of the roller 262 respectively extend out of corresponding ends of the cylindrical body 261, and the roller 262 is rotatably embedded in the corresponding mounting groove 251. The groove opening of the mounting groove 251 is vertically opened upwards (i.e. towards the bottom of the main body frame 1), and the assembling personnel or the maintenance personnel pull out the insertion barrel 261, so that the replacement or overhaul operation of the roller 26 can be completed, and the mounting groove has better practicability and convenience.
As shown in fig. 8 and 11, the seat body 23 is further provided with a positioning device 6 for locking the body frame 1 (see fig. 9) and the movable seat 2, the positioning device 6 includes a positioning shaft 61 and an elastic member 62 for driving the positioning shaft 61 to vertically extend out of the seat body 23, and a spring can be used as the elastic member 62, that is, the positioning shaft 61 can vertically extend out of the seat body 23 upwards under the elastic force of the elastic member 62 and is inserted and fixed with the bottom frame 15 of the body frame 1, so as to achieve the purpose of combining the locking body frame 1 and the movable seat 2. The positioning shaft 61 is vertically and slidably arranged in the saddle main body 23, and a limiting stop 611 is coaxially formed on the positioning shaft 61 in an extending manner; the bottom plate of the saddle main body 23 is fixedly provided with the limiting baffle 7, and the limiting baffle 7 is provided with a limiting groove 71, so that the elastic member 62 is coaxially sleeved on the positioning shaft 61 at the position below the limiting stop 611 and abuts against the bottom surface of the limiting stop 611, and the lower end of the elastic member 62 is embedded and fixed in the limiting groove 71, thereby achieving the purpose of driving the positioning shaft 61 to vertically slide.
Referring to fig. 11 and 12, a pedal upper bushing 63 is fixedly installed in the seat body 23, and the positioning shaft 61 is used in cooperation with the pedal upper bushing 63, that is, the positioning shaft 61 is vertically slidably disposed in the pedal upper bushing 63. As shown in fig. 9 and 11, an insertion positioning hole 151 is formed on the bottom plate of the bottom frame 15, and the insertion positioning hole 151 is used in cooperation with the positioning shaft 61; when the elastic member 62 drives the positioning shaft 61 to move vertically upward to protrude out of the seat main body 23, the positioning shaft 61 is simultaneously inserted into the insertion positioning hole 151 of the bottom frame 15, so as to realize the combined locking of the main body frame 1 and the seat main body 23.
Referring to fig. 11 and 12, the positioning shaft 61 is drivingly connected with a driving shaft 64, the driving shaft 64 is also vertically slidably disposed in the seat main body 23, a connecting plate 65 is connected between the positioning shaft 61 and the driving shaft 64, the connecting plate 65 is horizontally disposed, and two ends of the connecting plate 65 are respectively fixedly connected with the positioning shaft 61 and the driving shaft 64, that is, one end of the connecting plate 65 and the positioning shaft 61 are fixedly inserted and fixed above the limit stop 611, and the other end of the connecting plate 65 is fixedly connected with the upper end of the driving shaft 64. The inside installation of saddle main part 23 is fixed with footboard lower axle sleeve 66, and drive shaft 64 uses with the cooperation of footboard lower axle sleeve 66, and drive shaft 64 vertically slides and wears to locate in footboard lower axle sleeve 66 promptly, and the lower tip of drive shaft 64 has footboard 67 through the screw detachable connection, and footboard 67 horizontal installation is fixed. The lower part of the driving shaft 64 vertically extends downwards out of the saddle main body 23, the pedal plate 67 horizontally extends out of the bottom of the saddle main body 23, and an operator steps on the pedal plate 67 to enable the driving shaft 64 to vertically move downwards and synchronously drive the positioning shaft 61 to vertically move downwards through the connecting plate 65 until the positioning shaft 61 is separated from the inserting positioning hole 151, so that the purpose of separating the saddle main body 23 from the main body frame 1 is achieved. Adopt the biax structure of drive shaft 64 and location axle 61 cooperation use to realize the combination or the separation of saddle main part 23 and main body frame 1, operating personnel's foot is stepped on the effort and is indirectly acted on location axle 61 through drive shaft 64, is favorable to improving stability and the security that location axle 61 used.
Referring to fig. 8 and 11, a protective baffle 8 is vertically installed at one end of the movable seat 2 close to the positioning shaft 61, the pedal plate 67 horizontally penetrates through the protective baffle 8, and a notch 81 for the pedal plate 67 to move up and down is vertically formed on the protective baffle 8, so that the purpose that the pedal plate 67 drives the positioning device 6 is achieved. The both sides of guard flap 8 are vertical to be fixed with grafting post 82, and when running-board 67 was in the state of pushing down, operating personnel can set up the stopper in the centre gripping between grafting post 82 and running-board 67 to play the effect that restriction running-board 67 kick-backed, and the stopper can be pegged graft the cooperation with grafting post 82, in order to reach the purpose that further improves restriction running-board 67 motion stability.
The present embodiment is further described below with reference to specific actions:
the pedal 67 is stepped on by the operator, so that the driving shaft 64 vertically moves downwards, the positioning shaft 61 is synchronously driven by the connecting plate 65 to overcome the elastic driving force of the elastic piece 62 to vertically move downwards until the positioning shaft 61 is separated from the inserting positioning hole 151, and the purpose of separating the saddle main body 23 from the main body frame 1 is achieved, and a limiting block can be clamped between the inserting column 82 of the protective baffle plate 8 and the pedal 67 to prevent the pedal 67 from resetting, so that the purpose of facilitating the operator to complete the transferring operation of the movable saddle 2 is achieved; when the transfer operation of the movable seat 2 is completed or the movable seat 2 and the main body frame 1 need to be locked again, firstly, the limitation on the pedal 67 needs to be released, then the elastic member 62 extends to drive the positioning shaft 61 and the driving shaft 64 to synchronously move vertically and upwardly until the positioning shaft 61 is inserted into the insertion positioning hole 151, and the driving shaft 64 drives the pedal 67 to move vertically and upwardly along the gap 81.
Example three:
referring to FIGS. 1 and 13, a cell factory culture transfer cart is different from the first embodiment in that: the side dams 33 of unitary construction are replaced with side dams 33 of modular construction. In this embodiment, the side baffle 33 includes two support plates 34, ends of the two support plates 34 far away from each other are respectively rotatably fitted to outer ends of the corresponding outer support frames 31, and ends of the two support plates 34 close to each other are locked in a stacked manner. When the operator needs to take the cell factory 17 on the main body frame 1, the two plates 34 can be unlocked and the plates 34 can be turned over toward both sides, so that the operator can take the cell factory 17 in the main body frame 1 or place the cell factory 17 in the main body frame 1.
The side baffle 33 is changed into two short rods (namely two support plates 34) from an original whole long rod, but in the two structures, the number of the fixed points of the side baffle 33 is not changed, and the fixed points are five fixed points, wherein the two outermost fixed points are used as the rotating shafts of the support plates 34, the two second middle fixed points are used as the support shafts, the middle fixed points are used as hand-screwed bolts, and the two support plates 34 are folded and locked for use. Taking the original long rod bearing 1000N and the current plate 34 bearing 500N as an example, the following table (data unit is N):
| A | B | C | D | E | |
| original design | 281 | 146 | 160 | 134 | 223 |
| New design | 141 | 271 | 212 | 248 | 128 |
As can be seen from the above table, the original main force-bearing point is at the end positions on both sides of the side guard 33, and the present main force-bearing point is at the secondary center position of the side guard 33; the cell factory is fixed by a bolt (shaft pin) with the diameter of 5mm, the standard is A2-70, the tensile strength of the cell factory is not less than 700N, and the cell factory can completely meet the use requirement, namely, the cell factory 17 can be conveniently placed or taken by an operator while the basic stress condition is met.
The present embodiment is further described below with reference to specific actions:
when the cell factory 17 needs to be placed in the main body frame 1, the locking of the two support plates 34 needs to be firstly released, the support plates 34 are respectively turned over towards the two sides, then the cell factory 17 is placed in the main body frame 1, then the two support plates 34 are turned over reversely, so that the free ends of the two support plates 34 are respectively embedded and matched with the corresponding embedding grooves 341, meanwhile, the two support plates 34 are lapped on the corresponding step structures 35, so that the overlapping action of the two support plates 34 is completed, and finally, the locking support plates 34 and the middle support frame 32 are penetrated through by bolts, so that the locking action of the side baffle plates 33 is completed. When it is necessary to take out the cell factory 17 in the main body frame 1, the above-described operation is repeated to take out the cell factory 17 in the main body frame 1 and to lock the side shutter 33 again.
Example four:
as shown in fig. 14, a cell factory transfer method includes a transfer operation of a cell factory culture transfer vehicle, a transfer operation of a main body frame 1 storing a cell factory 17, and a filling and unloading operation of the cell factory 17, and specifically includes the following steps: s1. the cell factory 17 is filled in the main body frame 1 of the cell factory culture transfer trolley, and the cell factory 17 is fixed by a pressing component.
S2-1, transferring a cell factory culture transfer trolley filled with a cell factory 17 between adjacent thermostatic chambers or between a working chamber and the thermostatic chambers; after the culture is completed, the cell factory culture transfer trolley is transported out of the thermostatic chamber, the pressing assembly is unlocked, the support plate 34 is turned over, and the cell factory 17 on the main body frame 1 is unloaded.
S2-2, transferring the cell factory 17 to a thermostat by using a cell factory culture transfer trolley, unlocking the positioning device 6, and transferring the main body frame 1 filled with the cell factory 17 into the thermostat; after the culture is finished, the main body frame 1 filled with the cell factory 17 in the incubator is transferred to the movable vehicle seat 2, the main body frame 1 is locked through the positioning device 6, the cell factory culture transfer vehicle is transferred to a specified position, the pressing component is unlocked, the support plate 34 is overturned, and the cell factory 17 on the main body frame 1 is unloaded. As shown in fig. 15 and 16, a guide rail 9 used in cooperation with the movable seat 2 is provided in the oven in S2-2, the guide rail 9 includes a rail frame 91 and roller shafts 92 rotatably provided on the rail frame 91, guide blocks 93 are provided at equal intervals between the roller shafts 92, and the guide blocks 93 are in abutting sliding engagement with the guide grooves 16 of the main body frame 1.
S2-3, transferring the cell factory 17 to a thermostat or a thermostatic chamber by using a cell factory culture transfer trolley, unlocking the pressing component and turning over the support plate 34, and unloading the cell factory 17 on the main body frame 1 to the thermostat or the thermostatic chamber; after the culture is completed, the cell factory 17 is refilled in the main body frame 1, and transported to a designated position by using the cell factory culture transfer vehicle, the push-down assembly is unlocked and the support plate 34 is turned over, and the cell factory 17 on the main body frame 1 is unloaded.
The present embodiments are to be considered as illustrative and not restrictive, and modifications which do not constitute an inventive contribution to the present invention may be made as required by those skilled in the art after reading the present specification, but are protected by the patent laws within the scope of the appended claims.
Claims (10)
1. A cell factory culture transfer trolley comprising a main frame (1) for storing and transferring a cell factory (17), characterized in that: the bottom of the main body frame (1) is provided with a movable vehicle seat (2) which is matched with the main body frame for use, and the top of the main body frame (1) is provided with a pressing component for pressing down and locking the cell factory (17);
a split moving track (24) is formed on the movable saddle (2), the main body frame (1) is in sliding fit with the split moving track (24), and a positioning device (6) for fixing the position of the main body frame (1) is further arranged on the movable saddle (2); the positioning device (6) comprises a positioning shaft (61) and an elastic piece (62) for driving the positioning shaft (61) to extend out of the movable saddle (2), the positioning shaft (61) is matched with the movable saddle (2) in a telescopic sliding mode and is matched with the main body frame (1) in an inserting mode, and the elastic piece (62) is arranged in the movable saddle (2) in a telescopic mode;
the downward pressing assembly comprises a downward pressing plate (4) positioned above a vertical opening of a main body frame (1), the downward pressing plate (4) and the main body frame (1) are connected with a locking device (5), the locking device (5) comprises a turnover connecting plate (51) arranged on the main body frame (1), the turnover connecting plate (51) is connected with a tab extension plate (52), the tab extension plate (52) and the downward pressing plate (4) are connected with a tab (53), a hook block (54) is arranged on the tab extension plate (52), and the hook block (54) is in hook joint with the main body frame (1); upset connecting plate (51) and draw and detain extension plate (52) normal running fit, just draw and detain extension plate (52) and slide the cooperation with upset connecting plate (51) simultaneously, holding down plate (4) are in upset connecting plate (51), draw and detain arbitrary one side upset and the overlap joint in the outside of main body frame (1) of detaining extension plate (52) and the cooperation guide down of rotating and sliding of main body frame (1).
2. The cell factory culture transfer trolley of claim 1, wherein: a limiting stop block (611) is formed on the positioning shaft (61), a limiting baffle (7) is detachably mounted at the bottom of the movable vehicle seat (2), and a limiting groove (71) is formed in the limiting baffle (7); the elastic piece (62) is clamped between the limit stop (611) and the limit baffle (7), and two ends of the elastic piece (62) are respectively abutted against the limit stop (611) and the limit groove (71); the positioning shaft (61) is connected with a pedal (67), and the pedal (67) extends out of the movable vehicle seat (2).
3. A cell factory culture transfer trolley according to claim 2, characterized in that: the positioning shaft (61) is connected with a driving shaft (64) which is arranged in parallel with the positioning shaft, and the driving shaft (64) and the positioning shaft (61) move synchronously and are matched with the movable saddle (2) in a sliding way; the pedal (67) is mounted on the drive shaft (64), and the pedal (67) extends from the bottom of the movable seat (2) to the outside of the movable seat (2).
4. A cell factory culture transfer trolley according to claim 3, wherein: the movable type vehicle seat (2) is connected with a protective baffle (8), the pedal (67) penetrates through the protective baffle (8), and a channel (81) for the pedal (67) to move is formed in the protective baffle (8); the protective baffle (8) is provided with an inserting column (82), when the pedal plate (67) is in a pressing state, a limiting block is clamped between the inserting column (82) and the pedal plate (67), and the limiting block is in inserting fit with the inserting column (82).
5. The cell factory culture transfer trolley of claim 1, wherein: the split moving track (24) comprises rollers (26) rotatably arranged at the top of the movable vehicle seat (2), guide blocks (252) are arranged among the rollers (26) at intervals, a guide groove (16) is formed in the bottom plate of the main body frame (1) corresponding to the guide blocks (252), and the guide blocks (252) are in abutting sliding fit with the guide groove (16).
6. The cell factory culture transfer trolley of claim 1, wherein: two ends of the main body frame (1) in the length direction are respectively provided with a first handle (13), and one side of the movable vehicle seat (2) in the width direction of the main body frame (1) is provided with a second handle (14); an inserting block (22) is arranged on a side plate of the movable vehicle seat (2), and the second handle (14) is matched with the inserting block (22) in an inserting mode.
7. The cell factory culture transfer trolley of claim 1, wherein: the movable saddle (2) is provided with a support frame (3), the support frame (3) comprises outer support frames (31) positioned at two ends of the movable saddle (2) in the length direction, middle support frames (32) are arranged between the two outer support frames (31) at equal intervals, and the outer support frames (31) and the middle support frames (32) are connected with side baffles (33) extending along the length direction of the movable saddle (2); the side baffle (33) comprises two support plates (34), the two support plates (34) are respectively in running fit with the corresponding outer side support frames (31), and the support plates (34) and the middle support frame (32) are connected with locking pieces (342).
8. The cell factory culture transfer trolley of claim 1, wherein: a limiting piece (513) used for limiting the overturning position of the lower pressing plate (4) is arranged on the overturning connecting plate (51), a notch (524) is formed in the pull buckle extension plate (52), and the limiting piece (513) is in clamping fit with the notch (524); when the pulling buckle extension plate (52) is turned and clamped with the limiting part (513), the turning connection plate (51) is in butt fit with the opening groove of the hook block (54) in an embedded mode.
9. A cell factory transfer method using the cell factory culture transfer cart according to any one of claims 1 to 8, characterized in that: comprises the transferring operation of a cell factory culture transferring trolley, the transferring operation of a main body frame (1) storing a cell factory (17) and the filling and unloading operation of the cell factory (17), in particular comprises the following steps,
s1, filling a cell factory (17) in a main body frame (1) of a cell factory culture transfer trolley, and fixing the cell factory (17) through a pressing component;
s2-1, transferring a cell factory culture transfer trolley filled with a cell factory (17) between adjacent thermostatic chambers or between a working chamber and the thermostatic chambers; after the culture is finished, the cell factory culture transfer trolley is transported out of the thermostatic chamber, the pressing component is unlocked, the support plate (34) is turned over, and the cell factory (17) on the main body frame (1) is unloaded;
s2-2, transferring the cell factory (17) to a thermostat by using a cell factory culture transfer trolley, unlocking the positioning device (6), and transferring the main body frame (1) filled with the cell factory (17) into the thermostat; after the culture is finished, transferring the main body frame (1) filled with the cell factory (17) in the incubator to a movable vehicle seat (2), locking the main body frame (1) through a positioning device (6), transferring a cell factory culture transfer trolley to a specified position, unlocking a pressing component, overturning a support plate (34), and unloading the cell factory (17) on the main body frame (1);
s2-3, transferring the cell factory (17) into the incubator or the thermostatic chamber by using the cell factory culture transfer trolley, unlocking the pressing component and overturning the support plate (34), and unloading the cell factory (17) on the main body frame (1) into the incubator or the thermostatic chamber; after the culture is finished, the cell factory (17) is refilled on the main body frame (1), and is transported to a specified position by using a cell factory culture transfer trolley, the pressing-down component is unlocked, the support plate (34) is turned over, and the cell factory (17) on the main body frame (1) is unloaded.
10. A cell factory transport method according to claim 9, wherein: a guide rail (9) matched with the movable vehicle seat (2) is arranged in a thermostat in S2-2, the guide rail (9) comprises a rail frame (91) and roller shafts (92) rotatably arranged on the rail frame (91), guide blocks (93) are arranged between the roller shafts (92) at equal intervals, and the guide blocks (93) are abutted and matched with guide grooves (16) of a main body frame (1) in a sliding mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911304017.5A CN110962902B (en) | 2019-12-17 | 2019-12-17 | Cell factory culture transfer trolley and cell factory transfer method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911304017.5A CN110962902B (en) | 2019-12-17 | 2019-12-17 | Cell factory culture transfer trolley and cell factory transfer method |
Publications (2)
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| CN113355239B (en) * | 2021-06-20 | 2022-06-24 | 长春祈健生物制品有限公司 | Be used for cell factory to prepare biological product pipeline automation equipment |
| CN113306613A (en) * | 2021-07-09 | 2021-08-27 | 迪必尔生物工程(上海)有限公司 | Cell factory manipulator |
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| FR2325548A1 (en) * | 1975-09-26 | 1977-04-22 | Beizermann Michel | Food distribution trolley with cold food area - has removable secondary trolley to move selected food to oven |
| CN102341321B (en) * | 2009-03-12 | 2013-05-22 | 株式会社汤山制作所 | Medication tray conveying cart |
| CN104401631B (en) * | 2014-10-30 | 2016-05-18 | 重庆至信实业有限公司 | The containing rack of back door stiffener |
| CN205769465U (en) * | 2016-05-03 | 2016-12-07 | Tcl王牌电器(惠州)有限公司 | Tote cart |
| CN207670435U (en) * | 2017-08-29 | 2018-07-31 | 北京水木九天科技有限公司 | A kind of plant carrier being equipped with plant protection device |
| CN109229166B (en) * | 2018-07-30 | 2020-05-22 | 诸暨市众创机械设备厂 | Energy-saving material stacking and carrying device |
| CN209162075U (en) * | 2018-10-19 | 2019-07-26 | 辽宁成大生物股份有限公司 | A kind of cell factory frame |
| CN109251861B (en) * | 2018-10-19 | 2021-09-21 | 辽宁成大生物股份有限公司 | Method for preparing biological product by applying multi-axis robot to cell factory |
| CN209258159U (en) * | 2018-11-02 | 2019-08-16 | 郑州日产汽车有限公司 | Plate transports flexibility utensil |
| CN109532973A (en) * | 2018-12-12 | 2019-03-29 | 江苏蓝泽股份有限公司 | A kind of transport device of window sectional material |
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