CN110432190A - A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method - Google Patents
A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method Download PDFInfo
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- CN110432190A CN110432190A CN201910683653.7A CN201910683653A CN110432190A CN 110432190 A CN110432190 A CN 110432190A CN 201910683653 A CN201910683653 A CN 201910683653A CN 110432190 A CN110432190 A CN 110432190A
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 241000356842 Larimichthys polyactis Species 0.000 title claims abstract description 20
- 239000013535 sea water Substances 0.000 claims abstract description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 49
- 238000009395 breeding Methods 0.000 claims abstract description 12
- 230000001488 breeding effect Effects 0.000 claims abstract description 12
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 10
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 10
- 210000004681 ovum Anatomy 0.000 claims abstract description 10
- 238000010612 desalination reaction Methods 0.000 claims abstract description 9
- 230000004720 fertilization Effects 0.000 claims abstract description 4
- 235000013601 eggs Nutrition 0.000 claims description 34
- 230000032696 parturition Effects 0.000 claims description 15
- 239000004568 cement Substances 0.000 claims description 10
- 238000007667 floating Methods 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 10
- 241000195493 Cryptophyta Species 0.000 claims description 9
- 238000009287 sand filtration Methods 0.000 claims description 9
- 241000700141 Rotifera Species 0.000 claims description 8
- 230000012447 hatching Effects 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 5
- 235000002639 sodium chloride Nutrition 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 3
- 240000002900 Arthrospira platensis Species 0.000 claims description 3
- 229910000831 Steel Inorganic materials 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 230000002710 gonadal effect Effects 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 229940082787 spirulina Drugs 0.000 claims description 3
- 239000010959 steel Substances 0.000 claims description 3
- 235000008935 nutritious Nutrition 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 6
- 230000013020 embryo development Effects 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
- 241000251468 Actinopterygii Species 0.000 abstract description 3
- 230000000384 rearing effect Effects 0.000 abstract description 3
- 238000012364 cultivation method Methods 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000006213 oxygenation reaction Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 description 2
- 229920000153 Povidone-iodine Polymers 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 229960001621 povidone-iodine Drugs 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 1
- 208000026487 Triploidy Diseases 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method, belongs to seawater fish rearing of fingerling field.Characterized by the following steps: 1) spawning pond salinity adjusted；2) fertilization ovum collecting；3) high quality fertilized egg of scophthatmus separates；4) incubating oosperm；5) breeding water desalination and seed management.The little yellow croaker offspring seed cultivation method in Low-salinity sea area of the invention while also contributing to easily collecting high quality fertilized egg of scophthatmus it is possible to prevente effectively from high quality fertilized egg of scophthatmus influences since water salinity is droning in bottom of pond dead in embryonic development even embryo development procedure in spawning pond.Nursery pond salinity can make newly hatched larvae normal growth and development under minimum impact with specific speed reduction in this method, it is ensured that highest survival rate.
Description
Technical field
The invention belongs to seawater fish rearing of fingerling fields, and in particular to a kind of Low-salinity sea area little yellow croaker seed is high
Survival rate propagation method.
Background technique
Little yellow croaker fertilized eggs are buoyant egg, and at 26 or more seawater salinity, fertilized eggs float on water body upper layer, at this time fertilized eggs
Ability normal development, if seawater salinity is lower, floating state can not be presented in fertilized eggs, by the normal hair of extreme influence fertilized eggs
It educates, and then causes a large amount of fertilized eggs dead.It learns according to investigations, different regions seawater salinity has larger difference, such as Zhejiang sometimes
The coastal different sea area salinity in river are there are bigger difference, such as the island Zhoushan Of Zhejiang Province Deng Bu Sea Near seawater salinity 28 or so, and Zhejiang
Zhoushan Mount Dai island Sea Near salinity is between 17-22, in addition, the western Sea Near the Hu Gang seawater salinity of Xiangshan of Zhejiang Province is in 22.5-
Between 24.5；It can only be found from the investigation result of Along Zhejiang Coast, the salinity difference in different sea areas is huge.It can be seen that salinity
Relatively low sea area will be unable to be normally carried out little yellow croaker seed breeding, in consideration of it, the present invention adjusts effectively to solve this by salinity
One problem can obtain the high high-quality seed of little yellow croaker survived.
Summary of the invention
In view of the problems of the existing technology, it is an object of the invention to design to provide a kind of Low-salinity sea area little yellow croaker seedling
The technical solution of kind high-survival rate propagation method.
A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method, it is characterised in that including following step
It is rapid:
1) spawning pond salinity is adjusted
40h after parent population is hastened parturition passes through seawater salinity in addition sea salt adjusting spawning pond to 26.5；
2) fertilization ovum collecting
48h after hastening parturition collects floating fertilized eggs, the seawater flushing that the floating fertilized eggs salinity of collection is 26.5；
3) high quality fertilized egg of scophthatmus separates
The fertilized eggs of collection are transferred in the cylindrical glass steel wire bucket of bottom cone, add the sea that salinity is 26.5 in bucket in advance
Water stands 30min, collects floating fertilized eggs, disinfection, the seawater flushing that salinity is 26.5；
4) incubating oosperm
Isolated fertilized eggs are transferred to the cement pit that seawater salinity is 26.5 and are hatched；
5) breeding water desalination and seed management
The 6h after hatching membrane is slowly injected into nature seawater with the rate of the 1.5%/h of water body in pond, realizes seawater salinity
Desalination, and then reach normal seawater salinity, then subsequent seed management is carried out to prelarva.
A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method, it is characterised in that in the step 1)
Parent population is hastened parturition specifically: at 15-16 DEG C of nature seawater temperature, the parent population for selecting gonadal maturation carries out artificial induced spawning, hastens parturition
Parent population afterwards is put in a suitable place to breed in 4 × 5 × 1.5m3In spawning pond, parent population quantity in pond: 1 age 600-700 tail, 2 age 300-400 tails,
Sex ration column=2:1, change water 80% after hastening parturition, it is each primary to change water sooner or later daily.
A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method, it is characterised in that in the step 4)
It is long 5 × 7 × 1.6m that cement pit, which hatches specification,3Cement pit hatching, hatch water depth 75cm, each cement pit places fertilization
Ovum 4L.
A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method, it is characterised in that in the step 5)
Breeding water desalination specifically: after prelarva incubation of membrane 6h, process is added continuously with the speed of the 1.5%/h of water volume in pond
Dark precipitating and the nature seawater of sand filtration, gradually decrease seawater salinity in breeding spand, when water level rises to 1.5m, stop adding
Water；Dead ovum is removed by siphonage after incubation of membrane 72h, open-mouthed bait wheel animalcule is added in draining to the 1.2m depth of water, and adds concentration
Algae solution, wheel animalcule strengthen 12h using concentration algae and nutritious spirulina, continue continuously to add obscure shallow lake and sand filtration with the speed of 1.5%/h
Nature seawater, when water level rises to 1.5m, stop plus water；Hereafter it feeds wheel animalcule after draining 1/5 daily and adds concentration
Algae, and the obscure nature seawater to form sediment with sand filtration is added continuously with the speed of 1.5%/h, until reaching natural seawater salinity.
The little yellow croaker offspring seed cultivation method in Low-salinity sea area of the invention is it is possible to prevente effectively from high quality fertilized egg of scophthatmus in spawning pond
Death in embryonic development even embryo development procedure is influenced in bottom of pond since water salinity is droning, while also contributing to easily collecting
High quality fertilized egg of scophthatmus.Nursery pond salinity can make newly hatched larvae normal growth under minimum impact with specific speed reduction in this method
Development, it is ensured that highest survival rate.
Specific embodiment
Further illustrate the present invention with reference to embodiments.
Embodiment
1. spawning pond salinity is adjusted
At 15-16 DEG C of nature seawater temperature, the parent population for selecting gonadal maturation carries out artificial induced spawning, and the parent population after hastening parturition is put in a suitable place to breed
In 4 × 5 × 1.5m3In (length × width × height) cement pit, parent population quantity in pond: 1 age 600-700 tail；2 age 300-400 tails,
Sex ration column=2:1, change water 80% after hastening parturition, change that water is each primary, and 40h spawning pond passes through addition sea salt (sea after hastening parturition sooner or later daily
Water sunning obtains) seawater salinity 26.5 is adjusted, sea salt needs before spawning pond is added through 80 mesh mesh bag impurity screenings.
2. fertilized eggs are collected in seine
48h after hastening parturition collects fertilized eggs by seine, stops inflation 10-15min before seine, to float on water on high-quality ovum
Body upper layer, convenient for collecting；High-quality floating ovum is collected using the nylon net bag of 40 mesh, the floating ovum of collection is soft with 26.5 seawater
It rinses primary.
3. high quality fertilized egg of scophthatmus separates
Fertilized eggs after preliminary flushing are transferred to 0.5m3In the cylindrical glass steel wire bucket of bottom cone, seawater salinity is pre- in bucket
26.5 are first adjusted to, oxygenation 5min makes the fertilized eggs collected scatter, in order to prevent the too high influence separating effect of triploidy rate,
The amount of every barrel of placement fertilized eggs controls within 3L, stops oxygenation 30min, is sufficiently separated fertilized eggs high-quality and inferior, collects
The high quality fertilized egg of scophthatmus of floating, is sterilized using povidone iodine irrigation and disinfection, and rear 26.5 seawater is softly rinsed 3-4 times, thoroughly removed
Povidone iodine residual.
4. incubating oosperm
Fertilized eggs after disinfection cleaning are transferred to seawater salinity and are pre-adjusted to 26.5 5 × 7 × 1.6m3The water of (length × width × height)
Mud sump hatching, hatches water depth 75cm, and each cement pit places the high quality fertilized egg of scophthatmus 4L collected.
5. the desalination of newly hatched larvae aquaculture pond salinity and seed management
(1) determination of the optimum amount of water spraying
It puts each 200 tail of little yellow croaker prelarva that the hatching of same batch touches in a suitable place to breed in 12 300L experiment buckets, is divided into 4 groups, it is right respectively
Answering salinity is 17.5,20.0,23.5 and 26.5 groups, and every group 3 parallel, the initial salinity 26.5 of breeding barrel seawater, continuous uninterrupted
Oxygenation.After initial water body 150L, incubation of membrane 6h, according to 1.5%/h, 3%/h, 4.5%/h and 6%/h of water volume in experiment bucket
Rate of water injection carries out salinity adjusting, and the natural seawater salinity due to testing locating sea area is 23.5, so 17.5,20.0 and 26.5
Processing group adjusts acquisition by the fresh water after gas explosion and by the sea salt that seawater sunning obtains.From experimental result it can be found that with
The rate annotation of 1.5%/h adjusts salinity survival rate highest, and effect is best.
Influence (average value ± standard error) of the 1 different injection rate of table to newly hatched larvae survival rate
Note: the identical expression difference of same row letter is not significant, on the contrary then significant difference.
(2) breeding water desalination and seed rearing in hatchery
Through experiment it is found that when carrying out seed cultivation pool sea water desalination after prelarva incubation of membrane 6h, with 1.5% of water volume in pond/
Slowly continuously addition gradually decreases seawater salinity in breeding spand by dark precipitating and the nature seawater of sand filtration to the speed of h, to
When water level rises to 1.5m, stops adding water, lasted 72h apart from membrane at this time.
Dead ovum is removed by siphonage after incubation of membrane 72h, open-mouthed bait wheel animalcule (8-10 is added in draining to the 1.2m depth of water
A/mL), and concentration algae solution (1mL/100L) is added, wheel animalcule utilizes concentration algae (5g/100L) and spirulina (1g/100L) nutrition
Strengthen 12h, continues the nature seawater for slowly continuously adding obscure shallow lake and sand filtration with the speed of 1.5%/h, rise to 1.5m to water level
When, stop adding water.Hereafter it daily after draining 1/5, feeds wheel animalcule and adds concentration algae, and continuously added with the speed of 1.5%/h
The nature seawater in obscure shallow lake and sand filtration, continuous a couple of days, (natural seawater salinity 17.5 needed 14 days；Salinity is 20, needs 12
It；When salinity is 23.5,9 days are needed) after, water salinity and natural seawater salinity difference are within 0.2, to the intimate nothing of seed
It influences.Food transformation mode and later period domestication feed the methods of fish treasured feed and construct referring to a kind of little yellow croaker family of granted patent
And breeding superior families method, publication number CN106577382A.
The present invention is it is possible to prevente effectively from high quality fertilized egg of scophthatmus is sent out since water salinity is droning in bottom of pond influence embryo in spawning pond
Death in even embryo development procedure is educated, while also contributing to easily collecting high quality fertilized egg of scophthatmus.Nursery pond salinity is slow in the technology
The slow use for reducing technology can make first day of the first period of the hot season prelarva normal growth and development under minimum impact, it is ensured that highest survival rate.
The present invention gathers embodiment and is described, and those skilled in the art know, are not departing from essence of the invention
In the case where mind and range, various changes and equivalence replacement can be carried out to these features and embodiment.In addition, in the present invention
Introduction under, can modify to these features and embodiment to adapt to particular situation and material without departing from this hair
Bright spirit and scope.Therefore, the present invention is not limited by specific implementation example disclosed herein, fallen with the application's
Embodiment in scope of the claims belongs to protection scope of the present invention.
Claims (4)
1. a kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method, it is characterised in that the following steps are included:
1) spawning pond salinity is adjusted
40h after parent population is hastened parturition passes through seawater salinity in addition sea salt adjusting spawning pond to 26.5；
2) fertilization ovum collecting
48h after hastening parturition collects floating fertilized eggs, the seawater flushing that the floating fertilized eggs salinity of collection is 26.5；
3) high quality fertilized egg of scophthatmus separates
The fertilized eggs of collection are transferred in the cylindrical glass steel wire bucket of bottom cone, add the sea that salinity is 26.5 in bucket in advance
Water stands 30min, collects floating fertilized eggs, disinfection, the seawater flushing that salinity is 26.5；
4) incubating oosperm
Isolated fertilized eggs are transferred to the cement pit that seawater salinity is 26.5 and are hatched；
5) breeding water desalination and seed management
The 6h after hatching membrane is slowly injected into nature seawater with the rate of the 1.5%/h of water body in pond, realizes seawater salinity
Desalination, and then reach normal seawater salinity, then subsequent seed management is carried out to prelarva.
2. a kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method as described in claim 1, it is characterised in that institute
It states parent population in step 1) to hasten parturition specifically: at 15-16 DEG C of nature seawater temperature, the parent population for selecting gonadal maturation is carried out manually
It hastens parturition, the parent population after hastening parturition is put in a suitable place to breed in 4 × 5 × 1.5m3In spawning pond, parent population quantity in pond: 1 age 600-700 tail, 2 ages
300-400 tail, sex ration column=2:1, changes water 80% after hastening parturition, it is each primary to change water sooner or later daily.
3. a kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method as described in claim 1, it is characterised in that institute
Stating cement pit hatching specification in step 4) is long 5 × 7 × 1.6m3Cement pit hatching, hatch water depth 75cm, each cement
Place fertilized eggs 4L in pond.
4. a kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method as described in claim 1, it is characterised in that institute
It states breeding water in step 5) to desalinate specifically: after prelarva incubation of membrane 6h, connect with the speed of the 1.5%/h of water volume in pond
Continuous addition gradually decreases seawater salinity in breeding spand, rises to water level by dark precipitating and the nature seawater of sand filtration
When 1.5m, stop adding water；Dead ovum is removed by siphonage after incubation of membrane 72h, open-mouthed bait wheel is added in draining to the 1.2m depth of water
Worm, and concentration algae solution is added, wheel animalcule strengthens 12h using concentration algae and nutritious spirulina, and continuation is continuously added with the speed of 1.5%/h
The nature seawater for adding obscure shallow lake and sand filtration stops adding water when water level rises to 1.5m；Hereafter it is fed after draining 1/5 daily
Wheel animalcule simultaneously adds concentration algae, and the nature seawater in obscure shallow lake and sand filtration is continuously added with the speed of 1.5%/h, until reaching nature
Seawater salinity.
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| CN201910683653.7A CN110432190A (en) | 2019-07-26 | 2019-07-26 | A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method |
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| CN201910683653.7A CN110432190A (en) | 2019-07-26 | 2019-07-26 | A kind of Low-salinity sea area little yellow croaker seed high-survival rate propagation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110692557A (en) * | 2019-11-18 | 2020-01-17 | 浙江省农业科学院 | Method for cultivating yellow croaker by artificial seawater salinity desalination |
| CN113455426A (en) * | 2021-07-19 | 2021-10-01 | 宁德市鼎诚水产有限公司 | Breeding method of low-salt-resistant large yellow croaker |
| CN114009379A (en) * | 2021-12-14 | 2022-02-08 | 浙江省农业科学院 | Method and device for preparing desalted fingerlings of small yellow croakers |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110692557A (en) * | 2019-11-18 | 2020-01-17 | 浙江省农业科学院 | Method for cultivating yellow croaker by artificial seawater salinity desalination |
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| CN114009379A (en) * | 2021-12-14 | 2022-02-08 | 浙江省农业科学院 | Method and device for preparing desalted fingerlings of small yellow croakers |
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Application publication date: 20191112 |
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