CN110257225A - Isolating device and instrument for extracting nucleic acid - Google Patents
Isolating device and instrument for extracting nucleic acid Download PDFInfo
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 62
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 62
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 62
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 112
- 238000000605 extraction Methods 0.000 claims abstract description 62
- 230000007246 mechanism Effects 0.000 claims abstract description 59
- 238000007789 sealing Methods 0.000 claims description 20
- 238000005192 partition Methods 0.000 claims description 10
- 238000003860 storage Methods 0.000 abstract description 64
- 238000002955 isolation Methods 0.000 abstract description 34
- 230000004308 accommodation Effects 0.000 abstract description 24
- 206010011409 Cross infection Diseases 0.000 abstract description 4
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 4
- 239000000443 aerosol Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 208000005252 hepatitis A Diseases 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
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- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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Abstract
一种隔离装置及核酸提取仪,涉及核酸提取技术领域,所述隔离装置包括壳体,所述壳体内包含有两个或两个以上相互隔离的容纳区,所述容纳区用于容纳至少一个试剂容纳单元;所述壳体对应各所述容纳区分别设置有与所述容纳区连通的限位孔,所述限位孔的长度方向与所述试剂容纳单元的长度方向相同,所述限位孔与所述试剂容纳单元之间存在移动空间,所述移动空间用于供核酸提取机构的工作段移动。所述隔离装置通过多个容纳区将试剂容纳机构中的多个试剂容纳单元隔离,以降低其交叉感染可能性。
An isolation device and a nucleic acid extraction instrument, which relate to the technical field of nucleic acid extraction. The isolation device includes a casing, and the casing contains two or more mutually isolated accommodation areas, and the accommodation areas are used to accommodate at least one Reagent containing unit; the housing is respectively provided with limiting holes communicating with the containing regions corresponding to each containing region, the length direction of the limiting holes is the same as the length direction of the reagent containing unit, and the limiting holes are There is a movement space between the position hole and the reagent containing unit, and the movement space is used for the movement of the working section of the nucleic acid extraction mechanism. The isolation device isolates multiple reagent storage units in the reagent storage mechanism through multiple storage areas to reduce the possibility of cross-infection.
Description
技术领域technical field
本发明涉及核酸提取领域,具体而言,涉及一种隔离装置及核酸提取仪。The invention relates to the field of nucleic acid extraction, in particular to an isolation device and a nucleic acid extraction instrument.
背景技术Background technique
核酸是分子生物学研究的基本对象,核酸提取步骤是分子诊断中的基本环节。因此,核酸提取过程中提取到的核酸的质量对于分子诊断的结果影响极大。Nucleic acid is the basic object of molecular biology research, and the nucleic acid extraction step is the basic link in molecular diagnosis. Therefore, the quality of nucleic acid extracted during nucleic acid extraction has a great influence on the results of molecular diagnosis.
现有技术中,通常使用核酸提取仪进行核酸提取操作。核酸提取仪包括外壳,外壳内侧形成封闭腔室,封闭腔室内设置有试剂容纳机构和核酸提取机构,试剂容纳机构具有多个容纳槽,核酸提取机构依次插入多个放置有不同试剂的容纳槽,以进行核酸提取操作。为提高核酸提取效率,在壳体内可进行多样本的核酸提取操作,对应地,核酸提取机构包含多个提取件,多个提取件同时进行提取操作。In the prior art, a nucleic acid extractor is usually used for nucleic acid extraction. The nucleic acid extraction instrument includes a shell, and a closed chamber is formed inside the shell. A reagent holding mechanism and a nucleic acid extraction mechanism are arranged in the closed chamber. The reagent holding mechanism has a plurality of holding grooves, and the nucleic acid extraction mechanism is sequentially inserted into a plurality of holding grooves containing different reagents. for nucleic acid extraction. In order to improve the efficiency of nucleic acid extraction, nucleic acid extraction operations of multiple samples can be performed in the housing. Correspondingly, the nucleic acid extraction mechanism includes multiple extraction parts, and the multiple extraction parts perform extraction operations at the same time.
但是,在核酸提取作业过程中,反应可能产生气溶胶等物质,当一处产生气溶胶,气溶胶将在壳体内部四处流动,核酸提取机构上的多个提取件均会受到气溶胶影响,使得在后续检测过程中,核酸提取机构上的多个提取件提取出的物质均无法获得准确检测结果,例如甲肝、乙肝等病毒无法被检测出来。However, during the nucleic acid extraction process, the reaction may produce aerosols and other substances. When aerosols are generated at one place, the aerosols will flow around inside the housing, and multiple extraction parts on the nucleic acid extraction mechanism will be affected by the aerosols. As a result, in the subsequent detection process, the substances extracted by multiple extraction parts on the nucleic acid extraction mechanism cannot obtain accurate detection results, such as hepatitis A, hepatitis B and other viruses cannot be detected.
发明内容Contents of the invention
本发明的目的在于提供一种隔离装置和核酸提取仪,其能够在一定程度上减轻核酸提取过程中产生交叉污染。The purpose of the present invention is to provide an isolation device and a nucleic acid extraction instrument, which can reduce cross-contamination during nucleic acid extraction to a certain extent.
本发明的实施例是这样实现的:Embodiments of the present invention are achieved like this:
一种隔离装置,包括壳体,所述壳体内包含有两个或两个以上相互隔离的容纳区,所述容纳区用于容纳至少一个试剂容纳单元;An isolation device, comprising a housing, the housing contains two or more accommodation areas isolated from each other, and the accommodation areas are used to accommodate at least one reagent accommodation unit;
所述壳体对应各所述容纳区分别设置有与所述容纳区连通的限位孔,所述限位孔的长度方向与所述试剂容纳单元的长度方向相同,所述限位孔与所述试剂容纳件之间存在移动空间,所述移动空间用于供核酸提取机构的工作段移动。The housing is respectively provided with limiting holes communicating with the containing areas corresponding to the containing areas, the length direction of the limiting holes is the same as the length direction of the reagent containing unit, and the limiting holes are connected with the reagent containing units. There is a moving space between the reagent containing parts, and the moving space is used for the movement of the working section of the nucleic acid extraction mechanism.
在本发明较佳的实施例中,所述壳体包括本体和上盖,所述上盖盖合于所述本体,所述本体内设置有隔板,所述隔板将所述本体分隔为多个所述容纳区,所述限位孔设置于所述上盖,所述本体与所述上盖可拆卸连接。In a preferred embodiment of the present invention, the housing includes a body and an upper cover, the upper cover covers the body, a partition is arranged inside the body, and the partition separates the body into There are multiple accommodating areas, the limiting holes are arranged on the upper cover, and the body is detachably connected to the upper cover.
在本发明较佳的实施例中,所述本体与所述上盖之间设置有弹性密封垫,所述弹性密封垫对应于所述限位孔的区域设置有第一划口,所述第一划口的长度方向与所述限位孔的长度方向相同。In a preferred embodiment of the present invention, an elastic gasket is provided between the body and the upper cover, and the area of the elastic gasket corresponding to the limiting hole is provided with a first cutout, and the first slit is provided on the elastic gasket. The length direction of a notch is the same as the length direction of the limiting hole.
在本发明较佳的实施例中,所述弹性密封垫上设置有多个第二划口,所述第二划口与所述第一划口相交,各所述第二划口沿所述第一划口的长度方向间隔设置。In a preferred embodiment of the present invention, the elastic sealing pad is provided with a plurality of second slits, the second slits intersect with the first slits, and each of the second slits is along the first slit. The intervals in the length direction of a notch are set.
在本发明较佳的实施例中,所述弹性密封垫包括垫体,所述垫体上对应所述限位孔的区域设置有外凸部,所述外凸部伸入所述限位孔,所述第一划口设置于所述外凸部。In a preferred embodiment of the present invention, the elastic sealing pad includes a pad body, and a protruding portion is provided on the pad body corresponding to the limiting hole, and the protruding portion extends into the limiting hole , the first slit is disposed on the convex portion.
在本发明较佳的实施例中,所述本体与所述弹性密封垫之间设置有支撑板,所述支撑板上与所述限位孔相对的区域设置有长孔。In a preferred embodiment of the present invention, a support plate is provided between the body and the elastic sealing gasket, and a long hole is provided on the area of the support plate opposite to the limiting hole.
在本发明较佳的实施例中,所述隔板上设置有限位插块,所述弹性密封垫和所述壳体上均设置有限位插口,所述限位插块依次穿过所述弹性密封垫的限位插口后伸入所述壳体的限位插口。In a preferred embodiment of the present invention, a limit plug is provided on the partition, a limit socket is provided on the elastic gasket and the housing, and the limit insert passes through the elastic The limit socket of the sealing gasket extends into the limit socket of the housing.
在本发明较佳的实施例中,所述壳体上与所述限位孔相对的一侧设置有试剂孔,所述试剂孔用于供所述试剂容纳单元的部分区域伸出到所述壳体外侧。In a preferred embodiment of the present invention, a reagent hole is provided on the side of the housing opposite to the limiting hole, and the reagent hole is used to allow a part of the reagent containing unit to protrude into the shell outside.
在本发明较佳的实施例中,所述壳体由多个拆分盒连接形成,各所述拆分盒能够可拆卸连接,每个所述拆分盒具有一个所述容纳区。In a preferred embodiment of the present invention, the housing is formed by connecting a plurality of split boxes, each of which can be detachably connected, and each of the split boxes has one of the accommodating areas.
一种核酸提取仪,其包括外壳,所述外壳的内腔设置有核酸提取机构、试剂容纳机构和如上述技术方案所述的隔离装置,所述试剂容纳机构包含多个试剂容纳单元,所述试剂容纳单元设置有多个容纳槽,至少有一个试剂容纳单元中各容纳槽的开口均位于所述隔离装置内侧,所述核酸提取机构能够穿过所述隔离装置的限位孔伸入所述容纳槽。A nucleic acid extraction instrument, which includes a housing, the inner cavity of the housing is provided with a nucleic acid extraction mechanism, a reagent storage mechanism and an isolation device as described in the above technical solution, the reagent storage mechanism includes a plurality of reagent storage units, the The reagent storage unit is provided with a plurality of storage tanks, and the openings of each storage tank in at least one reagent storage unit are located inside the isolation device, and the nucleic acid extraction mechanism can extend into the isolation device through the limiting hole of the isolation device. Storage tank.
本发明实施例的有益效果是:The beneficial effects of the embodiments of the present invention are:
本申请提供的隔离装置能够应用于核酸提取仪中,核酸提取仪中设置有试剂容纳机构,试剂容纳机构包括多个试剂容纳单元,试剂容纳单元设置有多个容纳槽。隔离装置将核酸提取仪中的试剂容纳机构中的多个试剂容纳单元进行隔离,例如可以在每个容纳区仅放置一个试剂容纳单元,或者也可以在每个容纳区放置两个或更多个试剂容纳单元。在进行核酸提取的过程中,核酸提取机构可由限位孔伸入壳体,以插入一个试剂容纳单元中的某个容纳槽,并与容纳槽的试剂反应,在完成本次反应后,核酸提取机构移出该容纳槽,并移动到该试剂容纳单元中的另一个容纳槽进行下一步反应。The isolation device provided by the present application can be applied to a nucleic acid extraction instrument. The nucleic acid extraction instrument is provided with a reagent storage mechanism, the reagent storage mechanism includes a plurality of reagent storage units, and the reagent storage unit is provided with a plurality of storage tanks. The isolation device isolates multiple reagent storage units in the reagent storage mechanism in the nucleic acid extractor, for example, only one reagent storage unit can be placed in each storage area, or two or more reagent storage units can also be placed in each storage area Reagent holding unit. During the process of nucleic acid extraction, the nucleic acid extraction mechanism can extend into the casing through the limiting hole to be inserted into a certain storage tank in a reagent storage unit, and react with the reagent in the storage tank. After completing this reaction, the nucleic acid extraction The mechanism moves out of the storage tank, and moves to another storage tank in the reagent storage unit for the next step of reaction.
由于在核酸提取机构移动过程中,其与试剂接触的区域(工作段)始终位于壳体内的移动空间,因此,一方面,容纳区将多个试剂容纳单元进行隔离,使得即使部分试剂容纳单元处产生有气溶胶等物质,气溶胶也不会飘动到其他容纳区,从而可以减少与其他容纳区内的试剂容纳单元出现交叉感染情况,避免大面积感染情况发生;另一方面,由于核酸提取机构的工作段在移动过程中,始终位于对应的容纳腔的移动空间,因此即使该容纳空间内存在有气溶胶等物质,气溶胶等物质也不会吸附在试剂中并由核酸提取机构带到壳体外侧,从而可有效将气溶胶密封于该容纳腔,避免使得其他容纳腔内试剂感染。Since during the movement of the nucleic acid extraction mechanism, the area (working section) in contact with the reagent is always located in the moving space in the housing, therefore, on the one hand, the accommodation area isolates a plurality of reagent accommodation units, so that even if some reagent accommodation units are placed Aerosols and other substances are produced, and the aerosols will not float to other storage areas, thereby reducing cross-infection with reagent storage units in other storage areas and avoiding large-scale infection; on the other hand, due to the nucleic acid extraction mechanism During the moving process, the working section is always located in the moving space of the corresponding accommodation chamber, so even if there are aerosols and other substances in the accommodation space, the aerosols and other substances will not be adsorbed in the reagent and brought to the shell by the nucleic acid extraction mechanism. The outer side of the body, so that the aerosol can be effectively sealed in the accommodation cavity, so as to avoid infection of reagents in other accommodation chambers.
综上,使用上述隔离装置可将试剂容纳机构隔离在多个容纳区,减轻或避免不同容纳区内物质之间的交叉感染。To sum up, the use of the above isolation device can isolate the reagent storage mechanism in multiple storage areas, reducing or avoiding cross-infection between substances in different storage areas.
附图说明Description of drawings
为了更清楚地说明本发明实施例的技术方案,下面将对实施例中所需要使用的附图作简单地介绍,应当理解,以下附图仅示出了本发明的某些实施例,因此不应被看作是对范围的限定,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他相关的附图。In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention, and thus It should be regarded as a limitation on the scope, and those skilled in the art can also obtain other related drawings based on these drawings without creative work.
图1为本发明实施例提供的隔离装置与试剂容纳机构的装配示意图一;Figure 1 is a schematic diagram of the first assembly of the isolation device and the reagent containing mechanism provided by the embodiment of the present invention;
图2为本发明实施例提供的隔离装置与试剂容纳机构的装配示意图二;Fig. 2 is a second schematic diagram of the assembly of the isolation device and the reagent containing mechanism provided by the embodiment of the present invention;
图3为本发明实施例提供的隔离装置与试剂容纳机构的装配体的剖视图;3 is a cross-sectional view of the assembly of the isolation device and the reagent containing mechanism provided by the embodiment of the present invention;
图4为本发明实施例提供的隔离装置中本体、支撑板与试剂容纳机构的装配示意图;Fig. 4 is a schematic diagram of the assembly of the main body, the support plate and the reagent containing mechanism in the isolation device provided by the embodiment of the present invention;
图5为本发明实施例提供的隔离装置中本体与试剂容纳机构的装配示意图一;Fig. 5 is a first schematic diagram of the assembly of the body and the reagent containing mechanism in the isolation device provided by the embodiment of the present invention;
图6为本发明实施例提供的隔离装置中本体与试剂容纳机构的装配示意图二;Fig. 6 is a second schematic diagram of the assembly of the body and the reagent containing mechanism in the isolation device provided by the embodiment of the present invention;
图7为本发明实施例提供的隔离装置中本体的结构示意图一;Fig. 7 is a first structural schematic diagram of the body in the isolation device provided by the embodiment of the present invention;
图8为本发明实施例提供的隔离装置中本体的结构示意图二;Fig. 8 is the second structural schematic diagram of the body in the isolation device provided by the embodiment of the present invention;
图9为本发明实施例提供的隔离装置中弹性密封垫的结构示意图一;Fig. 9 is a first schematic structural view of the elastic gasket in the isolation device provided by the embodiment of the present invention;
图10为本发明实施例提供的隔离装置中弹性密封垫的结构示意图二;Fig. 10 is the second structural schematic diagram of the elastic gasket in the isolation device provided by the embodiment of the present invention;
图11为本发明实施例提供的隔离装置中上盖的结构示意图。Fig. 11 is a schematic structural view of the upper cover in the isolation device provided by the embodiment of the present invention.
图中:In the picture:
11-本体;12-上盖;13-隔板;131-限位插块;14-限位孔;15-卡块;16-试剂孔;20-弹性密封垫;21-第一划口;22-第二划口;23-外凸部;24-垫体;30-支撑板;31-长孔;40-限位插口;50-试剂容纳机构。11-body; 12-top cover; 13-partition; 131-limit plug; 14-limit hole; 15-block; 16-reagent hole; 20-elastic gasket; 22-the second notch; 23-the convex part; 24-the pad body; 30-the support plate; 31-the long hole; 40-the limit socket; 50-the reagent containing mechanism.
具体实施方式Detailed ways
为使本发明实施例的目的、技术方案和优点更加清楚,下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例是本发明一部分实施例,而不是全部的实施例。通常在此处附图中描述和示出的本发明实施例的个件可以通过各种不同的配置来布置和设计。In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. The individual pieces of the embodiments of the invention generally described and illustrated in the drawings herein may be arranged and designed in a variety of different configurations.
因此,以下对在附图中提供的本发明的实施例的详细描述并非旨在限制要求保护的本发明的范围,而是仅仅表示本发明的选定实施例。基于本发明中的实施例,本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。Accordingly, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.
应注意到:相似的标号和字母在下面的附图中表示类似项,因此,一旦某一项在一个附图中被定义,则在随后的附图中不需要对其进行进一步定义和解释。It should be noted that like numerals and letters denote similar items in the following figures, therefore, once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.
在本发明的描述中,需要说明的是,术语“中心”、“上”、“下”、“左”、“右”、“竖直”、“水平”、“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系,或者是该发明产品使用时惯常摆放的方位或位置关系,仅是为了便于描述本发明和简化描述,而不是指示或暗示所指的隔离装置或元件必须具有特定的方位、以特定的方位构造和操作,因此不能理解为对本发明的限制。此外,术语“第一”、“第二”、“第三”等仅用于区分描述,而不能理解为指示或暗示相对重要性。In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship that is usually placed when the product of the invention is used, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying Reference to isolating devices or elements must have a particular orientation, be constructed and operate in a particular orientation and therefore should not be construed as limiting the invention. In addition, the terms "first", "second", "third", etc. are only used for distinguishing descriptions, and should not be construed as indicating or implying relative importance.
此外,术语“水平”、“竖直”、“悬垂”等术语并不表示要求部件绝对水平或悬垂,而是可以稍微倾斜。如“水平”仅仅是指其方向相对“竖直”而言更加水平,并不是表示该结构一定要完全水平,而是可以稍微倾斜。In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the components are absolutely horizontal or overhanging, but may be slightly inclined. For example, "horizontal" only means that its direction is more horizontal than "vertical", and it does not mean that the structure must be completely horizontal, but can be slightly inclined.
在本发明的描述中,还需要说明的是,除非另有明确的规定和限定,术语“设置”、“安装”、“相连”、“连接”应做广义理解,例如,可以是固定连接,也可以是可拆卸连接,或一体地连接;可以是机械连接,也可以是电连接;可以是直接相连,也可以通过中间媒介间接相连,可以是两个元件内部的连通。对于本领域的普通技术人员而言,可以具体情况理解上述术语在本发明中的具体含义。In the description of the present invention, it should also be noted that, unless otherwise clearly specified and limited, the terms "setting", "installation", "connection" and "connection" should be understood in a broad sense, for example, it may be a fixed connection, It can also be a detachable connection or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediary, and it can be the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention in specific situations.
下面结合附图,对本发明的一些实施方式作详细说明。在不冲突的情况下,下述的实施例及实施例中的特征可以相互个合。Some embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. In the case of no conflict, the following embodiments and features in the embodiments can be combined with each other.
第一实施例first embodiment
请参照图1-图3、图11所示,本实施例提供一种隔离装置,包括壳体,壳体内包含两个或两个以上相互隔离的容纳区,容纳区用于容纳试剂容纳机构50中至少一个试剂容纳单元。Please refer to Fig. 1-Fig. 3 and Fig. 11. This embodiment provides an isolation device, which includes a housing, and the housing contains two or more accommodation areas isolated from each other, and the accommodation areas are used to accommodate the reagent accommodation mechanism 50. at least one reagent containing unit.
壳体对应各容纳区分别设置有限位孔14,限位孔14与对应的容纳区连通,限位孔14的长度方向与一个试剂容纳单元的长度方向相同,限位孔14与试剂容纳单元之间存在移动空间,移动空间用于供核酸提取机构的工作段移动。The housing is respectively provided with limiting holes 14 corresponding to each containing area, and the limiting holes 14 communicate with the corresponding containing areas. There is a moving space between them, and the moving space is used for moving the working section of the nucleic acid extraction mechanism.
具体地,在核酸提取仪中,包括有试剂容纳机构50和核酸提取机构,具体地,试剂容纳机构50包括有多个试剂容纳单元,每个试剂容纳件包含有多个容纳槽,各容纳槽沿试剂容纳单元的长度方向排列,在工作过程中,核酸提取机构中的核酸提取件依次插入某一个容纳槽,当核酸提取机构中包含有多个核酸提取件时,各核酸提取件可分别对应一个试剂容纳单元,如此设置,可在同一时间进行多个核酸提取工作。Specifically, in the nucleic acid extraction instrument, a reagent containing mechanism 50 and a nucleic acid extraction mechanism are included. Specifically, the reagent containing mechanism 50 includes a plurality of reagent containing units, and each reagent containing part includes a plurality of containing grooves, and each containing groove Arranged along the length direction of the reagent storage unit, during the working process, the nucleic acid extraction parts in the nucleic acid extraction mechanism are sequentially inserted into a certain storage tank. When the nucleic acid extraction mechanism contains multiple nucleic acid extraction parts, each nucleic acid extraction part can correspond to the A reagent containing unit is set up in this way, and multiple nucleic acid extraction tasks can be performed at the same time.
在隔离装置中,隔板13将壳体分为多个相互隔离的容纳区,试剂容纳机构50中的多个试剂容纳单元可分别放于不同的容纳区,从而将多个试剂容纳单元分成至少两部分进行隔离,不同容纳区内的试剂相互不会产生交叉感染。In the isolation device, the partition 13 divides the housing into a plurality of mutually isolated storage areas, and the multiple reagent storage units in the reagent storage mechanism 50 can be respectively placed in different storage areas, thereby dividing the multiple reagent storage units into at least The two parts are isolated, and the reagents in different holding areas will not produce cross-infection with each other.
壳体内容纳区的数量可以为两个或两个以上,每个容纳区至少容纳一个试剂容纳单元。举例来说,当需要壳体内最多能够容纳十个试剂容纳单元时,可采用下述几种设置方式,壳体内具有十个容纳区,各容纳区分别用于容纳一个试剂容纳单元。或者,壳体内具有五个容纳区,各容纳区分别用于容纳两个试剂容纳单元。或者,壳体内具有两个容纳区,各容纳区分别用于容纳五个试剂容纳单元。当然,除上述设置方式外,还有其他设置方式,在此不再赘述。The number of storage areas in the housing can be two or more, and each storage area can accommodate at least one reagent storage unit. For example, when it is required that the casing can accommodate at most ten reagent containing units, the following arrangements can be adopted. The casing has ten containing areas, and each containing area is used to accommodate one reagent containing unit. Alternatively, there are five accommodation areas in the casing, and each accommodation area is used to accommodate two reagent accommodation units respectively. Alternatively, there are two accommodation areas in the casing, and each accommodation area is used to accommodate five reagent accommodation units respectively. Of course, there are other setting methods besides the above-mentioned setting methods, which will not be repeated here.
在一种在本实施例中,壳体可以为整体结构,也可以包含多个拆分盒。In one embodiment, the casing may be of an integral structure, or may contain a plurality of split boxes.
当壳体包含多个拆分盒时,各拆分盒能够拆卸连接,每个拆分盒具有一个容纳区。如此设置,在使用过程中,可根据试剂容纳机构50内的试剂容纳单元的个数选择对应数量的拆分盒进行个装,以形成对应的壳体。例如,试剂容纳机构50包含三个试剂容纳单元,则将三个拆分盒依次连接,并将三个试剂容纳单元对应放入三个容纳区内。再例如,试剂容纳机构50包含四个试剂容纳单元,则将四个拆分盒依次连接,并将四个试剂容纳单元对应放入四个容纳区内。When the casing contains a plurality of split boxes, each split box can be detachably connected, and each split box has a receiving area. With such an arrangement, during use, a corresponding number of split cassettes can be selected for individual packaging according to the number of reagent storage units in the reagent storage mechanism 50 to form a corresponding housing. For example, if the reagent storage mechanism 50 includes three reagent storage units, the three split boxes are connected in sequence, and the three reagent storage units are correspondingly put into the three storage areas. For another example, if the reagent storage mechanism 50 includes four reagent storage units, the four split boxes are connected in sequence, and the four reagent storage units are correspondingly put into the four storage areas.
或者,在另一种使用场景中,虽然试剂容纳机构50包含四个试剂容纳单元,但是仅有其中两个试剂容纳单元中容纳有试剂,以供提取使用,则可使用两个拆分盒,分别将放置有试剂的两个试剂容纳单元对应放置于两个容纳区中。Or, in another usage scenario, although the reagent containing mechanism 50 includes four reagent containing units, but only two of the reagent containing units contain reagents for extraction, then two split boxes can be used, The two reagent storage units containing the reagents are correspondingly placed in the two storage areas.
当壳体为整体结构时,壳体包括可拆卸连接的本体11和上盖12,上盖12盖合于本体11,本体11内设置有隔板13,隔板13将本体11分隔为多个容纳区,限位孔14设置于上盖12。如此设置,便于向壳体内放置试剂容纳单元。当取放试管容纳单元时,将上盖12从本体11上取下,在取放动作结束后,将上盖12再次安装到本体11上。When the housing is an integral structure, the housing includes a detachably connected body 11 and an upper cover 12, the upper cover 12 covers the body 11, and a partition 13 is arranged inside the body 11, and the partition 13 separates the body 11 into a plurality of In the receiving area, the limiting hole 14 is disposed on the upper cover 12 . With such arrangement, it is convenient to place the reagent containing unit into the casing. When taking and placing the test tube containing unit, the upper cover 12 is removed from the main body 11 , and after the taking and placing action is completed, the upper cover 12 is installed on the main body 11 again.
上盖12与本体11之间的连接方式有多种,例如通过螺栓、销轴、卡扣等连接件连接,或者,如图4所示,上盖12与本体11之间为卡合连接,具体地,在上盖12上设置有卡槽,在本体11上对应卡槽的区域设置有卡块15,当将上盖12扣合于本体11后,卡块15伸入卡槽,从而将上盖12与本体11卡合。进一步地,上盖12的边缘设置有向本体11方向延伸的翻边,卡槽设置于翻边的内侧,卡块15设置于本体11的侧板外侧。在将上盖12扣合于本体11时,翻边包覆于本体11的上部区域外侧,卡块15伸入卡槽。当然,在另一种设置方式中,也可以将卡槽设置于本体11外侧,将卡块15设置于上盖12的翻边内侧。There are many connection methods between the upper cover 12 and the main body 11, such as connecting by bolts, pins, buckles, etc., or, as shown in FIG. Specifically, a card slot is provided on the upper cover 12, and a card block 15 is provided on the area corresponding to the card slot on the body 11. After the upper cover 12 is fastened to the body 11, the card block 15 extends into the card slot, thereby The upper cover 12 is engaged with the main body 11 . Further, the edge of the upper cover 12 is provided with a flange extending toward the main body 11 , the locking groove is disposed on the inner side of the flange, and the locking block 15 is disposed on the outer side of the side plate of the main body 11 . When the upper cover 12 is fastened to the main body 11 , the flange covers the outer side of the upper area of the main body 11 , and the locking block 15 extends into the locking slot. Of course, in another arrangement, the card slot can also be provided on the outside of the body 11 , and the card block 15 can be provided on the inside of the flange of the upper cover 12 .
如图1和图9所示,本体11与上盖12之间设置有弹性密封垫20,弹性密封垫20对应于限位孔14的区域设置有第一划口21,第一划口21的长度方向与限位孔14的长度方向相同。如此设置,在核酸提取机构未伸入壳体时,弹性密封件上第一划口21处呈闭合状态,使得弹性密封件完全将容纳区密封。当核酸提取机构伸入壳体时,核酸提取机构由第一划口21处伸入,弹性密封件上位于第一划口21旁的区域在核酸提取机构的挤压下变形,从而供核酸提取机构伸入。在核酸提取机构伸入壳体后,使用弹性密封件可使得限位孔14处出现的开口既能够供不同尺寸的核酸提取机构伸入,又可以保证开口尺寸相对更小。As shown in Figure 1 and Figure 9, an elastic gasket 20 is arranged between the body 11 and the upper cover 12, and the area of the elastic gasket 20 corresponding to the limiting hole 14 is provided with a first notch 21, the first notch 21 The length direction is the same as the length direction of the limiting hole 14 . In this way, when the nucleic acid extraction mechanism does not extend into the housing, the first slit 21 on the elastic sealing member is in a closed state, so that the elastic sealing member completely seals the accommodation area. When the nucleic acid extraction mechanism is stretched into the housing, the nucleic acid extraction mechanism is stretched in from the first notch 21, and the area next to the first notch 21 on the elastic seal is deformed under the extrusion of the nucleic acid extraction mechanism, thereby providing nucleic acid extraction Institutions reach in. After the nucleic acid extraction mechanism is inserted into the housing, the elastic seal can be used to allow the opening at the limiting hole 14 to be inserted by nucleic acid extraction mechanisms of different sizes, and to ensure that the opening is relatively smaller in size.
优选地,如图9所示,弹性密封垫20上设置有多个第二划口22,第二划口22与第一划口21相交,各第二划口22沿第一划口21的长度方向间隔设置。如此设置,第二划口22与第一划口21的相交处更易于形成更大的开口,以供核酸提取机构伸入。Preferably, as shown in FIG. 9 , the elastic gasket 20 is provided with a plurality of second slits 22 , the second slits 22 intersect the first slits 21 , and each second slit 22 is along the edge of the first slit 21 . Length direction interval setting. In this way, the intersection of the second notch 22 and the first notch 21 is more likely to form a larger opening for the nucleic acid extraction mechanism to enter.
在图9中,第一划口21与第二划口22垂直,且第一划口21与第二划口22相交处位于第二划口22的中部区域。In FIG. 9 , the first slit 21 is perpendicular to the second slit 22 , and the intersection of the first slit 21 and the second slit 22 is located in the middle area of the second slit 22 .
弹性密封垫20可以采用橡胶或硅胶材质制成,第一划口21和第二划口22均可由刀具在弹性密封垫20上沿直线切割形成。The elastic sealing pad 20 can be made of rubber or silicone material, and the first slit 21 and the second slit 22 can be formed by cutting the elastic sealing pad 20 along a straight line with a knife.
如图10所示,在一种具体实施方式中,弹性密封垫20包括垫体24,垫体24上对应限位孔14的区域设置有外凸部23,外凸部23伸入限位孔14,第一划口21设置于外凸部23。如此设置,由于弹性密封垫20位于上盖12的下方,因此当隔离装置装配完成后,弹性密封垫20的外凸部23可伸入上盖12的限位孔14内。外凸部23的设置便于将上盖12的限位孔14与弹性密封垫20上的第一划口21进行对位。As shown in FIG. 10, in a specific embodiment, the elastic sealing gasket 20 includes a pad body 24, and the area corresponding to the limiting hole 14 on the pad body 24 is provided with an outer convex portion 23, and the outer convex portion 23 extends into the limiting hole. 14 , the first notch 21 is disposed on the convex portion 23 . In this way, since the elastic gasket 20 is located under the upper cover 12 , the protruding portion 23 of the elastic gasket 20 can extend into the limiting hole 14 of the upper cover 12 after the isolation device is assembled. The setting of the protruding portion 23 is convenient for aligning the limiting hole 14 of the upper cover 12 with the first notch 21 on the elastic sealing gasket 20 .
如图3和图4所示,在一种优选实施方式中,本体11与弹性密封垫20之间设置有支撑板30,支撑板30上与限位孔14相对的区域设置有长孔31。具体地,长孔31与限位孔14一一对应设置。如此设置,支撑板30位于弹性密封垫20的下方,对于弹性密封垫20起到支撑作用,避免弹性密封垫20向容纳区内侧变形。支撑板30上的长孔31用于供穿过弹性密封垫20的核酸提取机构伸入容纳区。As shown in FIG. 3 and FIG. 4 , in a preferred embodiment, a support plate 30 is provided between the body 11 and the elastic gasket 20 , and a long hole 31 is provided on the support plate 30 opposite to the limiting hole 14 . Specifically, the elongated holes 31 are provided in one-to-one correspondence with the limiting holes 14 . In such a configuration, the supporting plate 30 is located below the elastic sealing pad 20 and plays a supporting role for the elastic sealing pad 20 to prevent the elastic sealing pad 20 from deforming toward the inside of the accommodation area. The long hole 31 on the support plate 30 is used for the nucleic acid extraction mechanism passing through the elastic sealing gasket 20 to extend into the accommodation area.
如图4-图7所示,隔板13上设置有限位插块131,弹性密封垫20和壳体上均设置有限位插口40,限位插块131依次穿过弹性密封垫20的限位插口40后伸入壳体的限位插口40。进一步地,在支撑板30上也设置有限位插口40,限位插块131依次穿过支撑板30上的限位插口40、弹性密封垫20的限位插口40后伸入壳体的限位插口40。如此设置,便于将本体11、上盖12、支撑板30和弹性密封件进行限位,防止上述结构相互之间出现窜动。此外,也可以通过限位插块131与限位插口40起到对位作用,使得限位孔14、长孔31、第一划口21和容纳区对位准确。As shown in Figures 4-7, the spacer 13 is provided with a limit insert 131, and the elastic gasket 20 and the housing are provided with a limit socket 40, and the limit insert 131 passes through the limit of the elastic gasket 20 in turn. The socket 40 extends into the limiting socket 40 of the housing. Further, a limit socket 40 is also provided on the support plate 30, and the limit insert 131 passes through the limit socket 40 on the support plate 30 and the limit socket 40 of the elastic gasket 20 in turn, and then extends into the limit of the housing. Socket 40. Such an arrangement facilitates positioning of the main body 11 , the upper cover 12 , the support plate 30 and the elastic sealing member, and prevents the above-mentioned structures from moving among each other. In addition, the position-limiting insert 131 and the position-limiting socket 40 can also be used for alignment, so that the positioning of the position-limiting hole 14 , the elongated hole 31 , the first notch 21 and the receiving area is accurate.
在本实施例的一种具体实施方式中,壳体内设置有固定架,试剂容纳单元放置于固定架上,并通过固定架与壳体相对固定。In a specific implementation of this embodiment, a fixing frame is arranged inside the casing, and the reagent containing unit is placed on the fixing frame, and is relatively fixed to the casing through the fixing frame.
或者,如图5和图8所示,在一种优选实施方式中,壳体上与限位孔14相对的一侧设置有试剂孔16,试剂孔16用于供试剂容纳单元的部分区域伸出到壳体外侧,但是试剂容纳单元中容纳槽的开口均位于容纳腔中。如此设置,使得壳体的尺寸可以进一步缩小,减少制造成本,降低隔离装置重量。Alternatively, as shown in Figures 5 and 8, in a preferred embodiment, a reagent hole 16 is provided on the side of the casing opposite to the limiting hole 14, and the reagent hole 16 is used for extending a part of the reagent containing unit. out to the outside of the housing, but the openings of the holding grooves in the reagent holding unit are located in the holding chamber. With such arrangement, the size of the casing can be further reduced, the manufacturing cost can be reduced, and the weight of the isolation device can be reduced.
优选地,壳体的试剂孔16被配置为,供试剂容纳单元的大部分区域伸出,仅容纳槽的开口处外沿区域搭设于壳体内侧,且与壳体该侧面的内壁接触。Preferably, the reagent hole 16 of the housing is configured such that most of the reagent containing unit protrudes, and only the outer edge area of the opening of the containing groove overlaps the inner side of the housing and contacts the inner wall of this side of the housing.
第二实施例second embodiment
本实施例提供一种核酸提取仪,其包括上述第一实施例提供的隔离装置,包括外壳,外壳的内腔设置有核酸提取机构、试剂容纳机构和隔离装置,试剂容纳机构50包括多个试剂容纳单元,试剂容纳单元设置有多个容纳槽,至少有一个试剂容纳单元中各容纳槽的开口均位于隔离装置内侧,核酸提取机构能够穿过隔离装置的限位孔伸入容纳槽。This embodiment provides a nucleic acid extraction instrument, which includes the isolation device provided in the first embodiment above, and includes a housing. The inner cavity of the housing is provided with a nucleic acid extraction mechanism, a reagent storage mechanism and an isolation device. The reagent storage mechanism 50 includes a plurality of reagents. The accommodating unit, the reagent accommodating unit is provided with a plurality of accommodating grooves, at least one opening of each accommodating groove in the reagent accommodating unit is located inside the isolating device, and the nucleic acid extraction mechanism can extend into the accommodating groove through the limiting hole of the isolating device.
也就是说,可以仅将其中一个试剂容纳单元放置于隔离装置内侧,以使得改个试剂容纳单元与其他试剂容纳单元隔离。也可以将多个试剂容纳单元分别放入隔离装置的不同容纳区,隔离为两部分或者多部分。That is to say, only one of the reagent containing units can be placed inside the isolation device, so that the other reagent containing unit is isolated from other reagent containing units. It is also possible to put a plurality of reagent storage units into different storage areas of the isolation device, and separate them into two or more parts.
优选地,各个试剂容纳单元均放置于隔离装置,且在隔离装置的各容纳区,最多放置有一个试剂容纳单元,使得各个试剂容纳单元相互之间均隔离。Preferably, each reagent storage unit is placed in the isolation device, and at most one reagent storage unit is placed in each storage area of the isolation device, so that each reagent storage unit is isolated from each other.
在一种具体实施方式中,试剂容纳单元包含多个试管,试管内设置有容纳槽。同一试剂容纳单元内的多个试管的开口外沿相连,形成联排管。In a specific embodiment, the reagent containing unit includes a plurality of test tubes, and containing grooves are arranged in the test tubes. The outer edges of the openings of the multiple test tubes in the same reagent containing unit are connected to form a series tube.
或者,试剂容纳单元可以为一个整体的块状结构,在该块状机构的其中一侧设置有一排容纳槽。Alternatively, the reagent containing unit may be an integral block structure, and a row of containing grooves is arranged on one side of the block structure.
以上所述仅为本发明的优选实施例而已,并不用于限制本发明,对于本领域的技术人员来说,本发明可以有各种更改和变化。凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110699231A (en) * | 2019-11-06 | 2020-01-17 | 中国人民解放军军事科学院军事医学研究院 | Reaction tube bottom support and nucleic acid extraction instrument |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130288259A1 (en) * | 2010-10-15 | 2013-10-31 | Universal Bio Research Co Ltd | Automated nucleic acid processor and automated nucleic acid processing method using multi function dispensing unit |
WO2014074740A1 (en) * | 2012-11-07 | 2014-05-15 | Life Technologies Corporation | Case for containing biological samples and corresponding method of use |
CN205133583U (en) * | 2015-10-29 | 2016-04-06 | 杭州爱津生物技术有限公司 | Nucleic acid extraction perforated plate box and supporting allying oneself with bar magnet cover more and lapping of using |
JP2017018053A (en) * | 2015-07-13 | 2017-01-26 | 凸版印刷株式会社 | Nucleic acid extraction kit, nucleic acid extraction method and nucleic acid extraction apparatus |
CN108102875A (en) * | 2016-11-24 | 2018-06-01 | 苏州百源基因技术有限公司 | A kind of heating structure and instrument for extracting nucleic acid applied in nucleic acid-extracting apparatus |
KR20180098089A (en) * | 2017-02-24 | 2018-09-03 | (주)바이오니아 | The kit for biochemical analysis by assembling a purification catridge with a panel selected from various pcr amplification panels |
CN208234923U (en) * | 2018-04-13 | 2018-12-14 | 浙江耶大生物医药有限公司 | The nucleic acid sequencing chip of liquid-leakage preventing |
KR20190001745A (en) * | 2017-06-28 | 2019-01-07 | 주식회사 파나진 | Kit for specimen-reagent response analysis, target nucleic acids detection apparatus and method using the same |
CN109229878A (en) * | 2018-07-27 | 2019-01-18 | 山东见微生物科技有限公司 | sample container and sample processing device |
CN210314220U (en) * | 2019-08-06 | 2020-04-14 | 中国人民解放军军事科学院军事医学研究院 | Isolation device and nucleic acid extraction instrument |
-
2019
- 2019-08-06 CN CN201910723629.1A patent/CN110257225A/en active Pending
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130288259A1 (en) * | 2010-10-15 | 2013-10-31 | Universal Bio Research Co Ltd | Automated nucleic acid processor and automated nucleic acid processing method using multi function dispensing unit |
WO2014074740A1 (en) * | 2012-11-07 | 2014-05-15 | Life Technologies Corporation | Case for containing biological samples and corresponding method of use |
JP2017018053A (en) * | 2015-07-13 | 2017-01-26 | 凸版印刷株式会社 | Nucleic acid extraction kit, nucleic acid extraction method and nucleic acid extraction apparatus |
CN205133583U (en) * | 2015-10-29 | 2016-04-06 | 杭州爱津生物技术有限公司 | Nucleic acid extraction perforated plate box and supporting allying oneself with bar magnet cover more and lapping of using |
CN108102875A (en) * | 2016-11-24 | 2018-06-01 | 苏州百源基因技术有限公司 | A kind of heating structure and instrument for extracting nucleic acid applied in nucleic acid-extracting apparatus |
KR20180098089A (en) * | 2017-02-24 | 2018-09-03 | (주)바이오니아 | The kit for biochemical analysis by assembling a purification catridge with a panel selected from various pcr amplification panels |
KR20190001745A (en) * | 2017-06-28 | 2019-01-07 | 주식회사 파나진 | Kit for specimen-reagent response analysis, target nucleic acids detection apparatus and method using the same |
CN208234923U (en) * | 2018-04-13 | 2018-12-14 | 浙江耶大生物医药有限公司 | The nucleic acid sequencing chip of liquid-leakage preventing |
CN109229878A (en) * | 2018-07-27 | 2019-01-18 | 山东见微生物科技有限公司 | sample container and sample processing device |
CN210314220U (en) * | 2019-08-06 | 2020-04-14 | 中国人民解放军军事科学院军事医学研究院 | Isolation device and nucleic acid extraction instrument |
Non-Patent Citations (2)
Title |
---|
OZLEM YAREN 等: "Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya", 《BMC INFECTIOUS DISEASES》, vol. 293, no. 17, pages 1 - 13 * |
张开慧等;: "新一代的磁珠法核酸自动提取仪研究进展", 《甘肃畜牧兽医》, vol. 49, no. 04, pages 53 - 56 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110699231A (en) * | 2019-11-06 | 2020-01-17 | 中国人民解放军军事科学院军事医学研究院 | Reaction tube bottom support and nucleic acid extraction instrument |
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