CN215975807U

CN215975807U - Reaction bin

Info

Publication number: CN215975807U
Application number: CN202122410910.5U
Authority: CN
Inventors: 梅哲; 张彤
Original assignee: Guangzhou Wondfo Biotech Co Ltd
Current assignee: Guangzhou Wondfo Biotech Co Ltd
Priority date: 2021-09-30
Filing date: 2021-09-30
Publication date: 2022-03-08
Anticipated expiration: 2031-09-30

Abstract

The utility model discloses a reaction bin which comprises a bin body, a central piece and a reaction bin chamber, wherein the bin body is provided with a mounting hole, a partition plate is arranged in the mounting hole, the wall of the mounting hole is annularly provided with a plurality of barrel bodies, peripheral chambers are formed in the barrel bodies, the partition plate is provided with a first through hole, a piston mechanism is hermetically arranged in each peripheral chamber, a first through hole is formed in each peripheral chamber on the partition plate, and the first through holes are communicated with the peripheral chambers and the first through holes; the center piece penetrates through the first through hole and is in sealed connection with the partition plate, the center piece and/or the bin body rotate around the center of the first through hole, a central cavity is arranged in the center piece, a second communication hole communicated with the central cavity is formed in the center piece, and the second communication hole is selectively communicated with one of the first communication holes; the reaction chamber is communicated with the central cavity and/or at least one peripheral cavity, and a heating mechanism is arranged in the reaction chamber and/or the peripheral cavity. The structure is simple, and the analyte sample is convenient to manufacture.

Description

Reaction bin

Technical Field

The utility model relates to the technical field of medical detection instruments, in particular to a reaction bin.

Background

Early detection of infection, such as at the time of an epidemic outbreak, affects not only the health of an individual, but also public health. Such detection requires a cost-effective, reliable and specific assay. Because of these needs, such assays have traditionally been performed in centralized laboratories, rather than on-the-fly. However, the instant analysis may bring the test to a test subject in the field or clinic, providing faster detection of the test subject and public health.

During the course of an assay, a reaction device is often required to subject a biological sample to a series of reactions to produce an analyte sample for detection. The existing reaction device has a complex structure and great limitation, and can not meet the detection requirement.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model aims to: provides a reaction chamber which has simple structure and is convenient for manufacturing an analyte sample.

To achieve the purpose, the embodiment of the utility model adopts the following technical scheme:

providing a reaction chamber comprising:

the bin body is provided with mounting holes in a penetrating mode along a first direction, partition plates are arranged in the mounting holes and are adjacent to the lower end of the bin body, a plurality of barrel bodies are annularly fixed on the hole walls of the mounting holes, peripheral chambers are formed in the barrel bodies, the lower ends of the barrel bodies are connected with the partition plates, a first through hole is formed in the center of each partition plate, a piston mechanism capable of moving along the first direction is hermetically arranged in each peripheral chamber, a first connecting hole is formed in each partition plate corresponding to each peripheral chamber, and the first connecting holes are communicated with the peripheral chambers and the first through holes;

the center piece penetrates through the first through hole and is in sealed connection with the partition plate, the center piece and/or the bin body rotate around the center of the first through hole, a central cavity is arranged in the center piece, an opening of the central cavity is located at the upper end of the center piece, a second communication hole communicated with the central cavity is formed in the center piece, and the second communication hole is selectively communicated with one of the first communication holes;

the reaction bin is communicated with the central cavity and/or at least one peripheral cavity, the reaction bin is positioned in the mounting hole and positioned between the central cavity and the peripheral cavity, and a heating mechanism is arranged in the reaction bin and/or the peripheral cavity.

The embodiment of the utility model has the beneficial effects that: through the storehouse body that sets up a plurality of periphery cavities and the centre piece of central cavity, can introduce into the central cavity with biological sample in, then relative rotation between through storehouse body and the centre piece, make the central cavity communicate with different periphery cavities in proper order, can set up different reaction mixture according to the order of preparation analyte sample in the corresponding periphery cavity, for example the lysis buffer solution that sets gradually, combine buffer solution, first washing buffer solution, second washing buffer solution and elution buffer solution, make biological sample automatic preparation be the analyte sample, and then detect. And piston mechanism can be with the biological sample pump income in the central cavity to the peripheral cavity interior operation such as mix, reaction, washing of corresponding, can also be with corresponding in the liquid pump withdrawal central cavity that the operation ended to and in the liquid pump income reaction storehouse that obtains the preparation, simplified analyte sample preparation process, provide the preparation efficiency height, can promote detection efficiency, satisfy the demand that detects immediately.

Drawings

The utility model is explained in more detail below with reference to the figures and examples.

FIG. 1 is a schematic view of an angle configuration of the cartridge body according to the embodiment.

Fig. 2 is a schematic view of another angle of the cartridge body according to the present embodiment.

Fig. 3 is a schematic structural view of the center piece according to the embodiment.

Fig. 4 is a cross-sectional view of a centerpiece according to an embodiment.

FIG. 5 is a structural diagram of a reaction chamber according to an embodiment.

FIG. 6 is a block diagram of a first seal according to an embodiment.

FIG. 7 is a block diagram of a second seal according to an embodiment.

Fig. 8 is a structural view of the lower cover according to the embodiment.

Fig. 9 is a structural view of the upper cover according to the embodiment.

Fig. 10 is a structural view of a heat conductor according to an embodiment.

Fig. 11 is a structural view of the piston rod according to the embodiment (the stopper is not shown).

FIG. 12 is a block diagram of a sample container according to an embodiment.

FIG. 13 is a diagram of the first driver and piston mechanism according to one embodiment.

Fig. 14 is a schematic view of the connection of the second driving member to the center member according to the embodiment.

In the figure:

1. a bin body; 101. a partition plate; 102. a barrel; 103. a peripheral chamber; 104. a vent; 105. a first communication hole; 106. detecting a window; 107. a first through hole;

2. a center piece; 201. a first notch; 202. a second recess; 203. a central chamber; 204. a step surface; 205. a second communication hole;

3. a piston rod; 301. connecting grooves;

4. a first seal member; 401. a first insertion tube; 402. a fourth communication hole; 403. a second insertion tube;

5. a second seal member;

6. a lower cover; 601. an aperture;

7. an upper cover; 701. a second through hole; 702. a third recess;

8. a first driving member; 801. a first rotary motor; 802. a rack; 803. a connecting portion;

9. a second driving member; 901. rotating the spline; 902. magnetically attracting the device; 903. a second rotary motor;

10. a heat conductor; 1001. a first stage; 1002. a second stage; 1003. a card slot;

11. a sample container; 1101. a bottle body; 1102. a first protrusion; 1103. a third protrusion; 1104. a valve plug; 1105. a valve seat.

Detailed Description

In order to make the technical problems solved, technical solutions adopted and technical effects achieved by the present invention clearer, the technical solutions of the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, removably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in fig. 1 to 6, the present invention provides a reaction bin, which comprises a bin body 1, a central piece 2 and a reaction bin chamber, wherein the bin body 1 is provided with a mounting hole along a first direction, and a partition board 101 is arranged in the mounting hole. The baffle 101 is close to the lower extreme of storehouse body 1, and the pore wall ring of mounting hole is fixed with a plurality of barrels 102, forms peripheral cavity 103 in the barrel 102, and the lower extreme of barrel 102 is connected with baffle 101, and first through-hole 107 is seted up at the center of baffle 101. The peripheral chambers 103 are internally and hermetically provided with a piston mechanism capable of moving along a first direction, the partition plate 101 is provided with a first communication hole 105 corresponding to each peripheral chamber 103, the first communication hole 105 is communicated with the peripheral chambers 103 and a first through hole 107, and the central part 2 passes through the first through hole 107 and is hermetically connected with the partition plate 101. In this specification, the first direction refers to a vertical direction. The centerpiece 2 and/or the cartridge body 1 rotate about the center of the first through hole 107 so that the centerpiece 2 can rotate with respect to the cartridge body 1. The central member 2 is provided with a central chamber 203 therein, the opening of the central chamber 203 is positioned at the upper end of the central member 2, the central member 2 is opened with a second communication hole 205 communicating with the central chamber 203, and the second communication hole 205 is selectively communicated with one of the first communication holes 105. It should be noted that the center piece 2 can rotate relative to the cabin body 1, in the first case, the cabin body 1 is stationary, and the center piece 2 rotates around the center of the first through hole 107; in the second situation, the central piece 2 is static, and the bin body 1 rotates around the center of the first through hole 107; in the third case, the cartridge body 16 and the centerpiece 2 rotate simultaneously about the center of the first through-hole 107, but at different speeds. The center piece 2 and the bin body 1 are relatively rotated, so that the second communication hole 205 is selectively communicated with one of the first communication holes 105, and the liquid communication between the central chamber 203 and the peripheral chamber 103 is opened or closed. The reaction chamber is communicated with the central cavity 203 and/or the at least one peripheral cavity 103, the reaction chamber is positioned in the mounting hole and positioned between the central cavity 203 and the peripheral cavity 103, and a heating mechanism is arranged in the reaction chamber and/or the peripheral cavity 103. The heating mechanism provides the reaction chamber or peripheral chamber 103 with the temperature required for the reaction. Through the storehouse body 1 and the centre piece 2 of central cavity 203 that set up a plurality of periphery cavities 103, can introduce into central cavity 203 with biological sample, then relative rotation between storehouse body 1 and the centre piece 2, make central cavity 203 communicate with different periphery cavities 103 in proper order, can set up different reaction mixture according to the order of preparation analyte sample in the corresponding periphery cavity 103, for example the lysis buffer solution that sets gradually, combine the buffer solution, first washing buffer solution, second washing buffer solution and elution buffer solution, make biological sample automatic preparation be the analyte sample, and then detect. And piston mechanism can pump into the operation such as mixing, reaction, washing in the corresponding periphery cavity 103 with the biological sample pump in the central cavity 203, can also pump back central cavity 203 with the liquid that corresponds the operation and finish to and the liquid pump that obtains the preparation is pumped in the reaction storehouse, simplified analyte sample preparation process, provide the preparation efficiency height, can promote detection efficiency, satisfy the demand that detects immediately.

In order to make the second communication hole 205 and the first communication hole 105 be combined in a sealing manner, an elastic sealing ring is provided at an orifice of the second communication hole 205, and specifically, the elastic sealing ring is an O-ring.

In this embodiment, six cylinder bodies 102 are annularly fixed on the hole wall of the mounting hole, so that six peripheral chambers 103 are annularly distributed in the interior of the cabin body 1, and the six peripheral chambers 103 are a first peripheral chamber, a second peripheral chamber, a third peripheral chamber, a fourth peripheral chamber, a fifth peripheral chamber and a sixth peripheral chamber in sequence, and one central chamber is provided. Wherein the first, second, third, fourth and fifth peripheral chambers are all provided with a reaction mixture therein, the sixth peripheral chamber is not provided with a reaction mixture therein, and the sixth peripheral chamber is provided with a piston mechanism and air therein. In this embodiment, two reaction chambers are disposed in the mounting hole, one cylinder 102 is disposed between the two reaction chambers, and the two reaction chambers are respectively communicated with a peripheral chamber 103 of the cylinder 102 located therebetween through a third communication hole. In this embodiment, the outer peripheral chamber 103 between the two reaction chambers is a sixth outer peripheral chamber, and the sixth outer peripheral chamber is communicated with the reaction chamber through the third communication hole. When the sixth outer peripheral chamber is communicated with the central chamber 203, the sixth outer peripheral chamber, the reaction chamber and the central chamber 203 are communicated with each other, and the liquid inside the central chamber 203 can be transferred into the reaction chamber by the piston mechanism inside the sixth outer peripheral chamber.

Specifically, the reaction mixture inside the first peripheral chamber comprises a lysis buffer, the reaction mixture inside the second peripheral chamber comprises a first elution buffer, the reaction mixture inside the third peripheral chamber comprises a second elution buffer, and the reaction mixture inside the fourth peripheral chamber comprises an elution buffer. The piston mechanism inside the sixth peripheral chamber is used for the introduction of the liquid inside the subsequent central chamber 203 into the reaction chamber. Of course, in other embodiments, specific reagents or gases may be placed in the first peripheral chamber, the second peripheral chamber, the third peripheral chamber, the fourth peripheral chamber, the fifth peripheral chamber and the sixth peripheral chamber according to actual needs.

Of course, in other embodiments, the number of the cylinders 102 and the number of the central chambers 203 can be flexibly adjusted as needed to meet the requirements of analysis and detection, for example, eight cylinders 102 and two central chambers 203 are provided, and both central chambers 203 can be selectively communicated with the peripheral chamber 103 selectively inside any one cylinder 102.

The first through hole 107 provides a space for the center piece 2 to pass through the partition 101, and during the rotation process of the center piece 2 around the center of the first through hole 107, in order to avoid the hole wall of the first through hole 107 from causing position interference on the center piece 2 and ensure the smooth rotation of the center piece 2, the position where the center piece 2 is matched with the first through hole 107 is cylindrical.

Wherein, referring to fig. 11 and 13, the upper end of the piston rod 3 has a piston head for sealing the peripheral chamber 103, and the lower end of the piston rod 3 has a connecting groove 301, the connecting groove 301 being for connecting the first driver 8 for driving the piston mechanism to move. Specifically, the inner side wall of the peripheral chamber 103 is provided with an elastic seal portion by which the piston head is sealed inside the peripheral chamber 103. The first driver 8 is connected with the piston rod 3 by a connecting groove 301, the first driver 8 being usable for driving the piston mechanism to move in a first direction, i.e. in a vertical direction, in the peripheral chamber 103.

The first driving member 8 includes a first rotating motor 801 and a rack 802, an output shaft of the first rotating motor 801 is connected to the rack 802, the output shaft 801 through the first rotating motor can drive the rack 802 to ascend and descend along a vertical direction, and a connecting portion 803 cooperatively connected with the connecting groove 301 is provided at a lower end of the rack 802. The connecting portion 803 on the rack 802 is connected with the connecting groove 301 on the piston rod 3, so that the first driving member 8 and the piston rod 3 are connected into a whole, and the lifting of the rack 802 drives the lifting of the piston rod 3.

In an embodiment, the piston rods 3 in all the piston mechanisms share one first driving member 8, the first driving member 8 is connected with a translation component, the translation component can move the first driving member 8 to the corresponding piston mechanism, and the connecting portion 803 on the rack 802 is connected with the connecting groove 301 on the corresponding piston rod 3 in a matching manner, so that the number of the first driving members 8 is effectively reduced, and the equipment cost is saved.

In another embodiment, there is one first driver 8 for each piston mechanism, and the rack 802 in each first driver 8 is connected to the piston rod 3 in the corresponding piston mechanism.

In this embodiment, the center piece 2 includes an upper portion and a lower portion connected to the upper portion, the cross-sectional dimension of the upper portion is larger than that of the lower portion, a step surface 204 is formed between the upper portion and the lower portion, the step surface 204 abuts against the upper surface of the partition plate 101, and the step surface 204 prevents the center piece 2 from falling off the partition plate 101.

In order to reduce the liquid retention in the central chamber 203, the bottom of the central chamber 203 is inclined, the second communication hole 205 is communicated with the lowest position of the bottom of the central chamber 203, and the liquid in the central chamber 203 can be discharged through the second communication hole 205 to the maximum extent.

Referring to fig. 4, the second communication hole 205 is inclined downward from the end connecting the central chamber 203 toward the end away from the central chamber 203. Specifically, the upper part has an upper chamber inside, the lower part has a lower chamber inside communicating with the upper chamber, the upper chamber is cylindrical, and the bottom of the lower chamber is inclined downward toward the end far away from the central chamber 203 to form a funnel structure. The central chamber 203 of such a structure facilitates the discharge of the internal biological sample through the second communication hole 205.

Referring to fig. 3 and 12, the upper end of the core member 2 is opened with a first recess 201, and the first recess 201 is used for connecting the sample container 11. It will be appreciated that the reaction chamber is used in conjunction with a sample container 11. Wherein a sample container 11 is arranged in the central chamber 203, the sample container 11 internally containing a biological sample for detection. The sample container 11 comprises a bottle body 1101 and a sealing body, a sample chamber for containing a sample solution is arranged in the bottle body 1101, an opening is formed in the upper end of the sample chamber, a valve body is arranged at the lower end of the sample chamber, the valve body comprises a valve seat 1105 and a valve plug 1104, the valve cavity of the valve seat 1105 is selectively plugged by the valve plug 1104, the valve seat 1105 is fixedly connected with the bottom of the sample chamber, a first bulge 1102 is arranged on one side, away from the sample chamber, of the valve plug 1104, the first bulge 1102 is connected with the first notch 201 in a matched mode, and the valve plug 1104 can be driven to rotate relative to the valve seat 1105 through the rotation center part 2, so that the interior of the sample chamber is communicated with the exterior. The first protrusion 1102 is coupled to the first recess 201, and when the central member 2 is rotated, the valve plug 1104 is driven to rotate synchronously, so that the valve cavity is opened, the interior of the bottle 1101 is communicated with the central chamber 203, and the biological sample in the bottle 1101 can flow into the central chamber 203. When the valve cavity is closed, the biological sample inside the sample chamber is trapped inside the sample container 11. By placing the sample container 11 into the central chamber 203, the biological sample inside the sample container 11 can be isolated from the external environment, the external environment is prevented from polluting the biological sample, and the biological sample is prevented from diffusing into the external environment, when the biological sample is required to react inside the reaction chamber, the valve cavity at the bottom of the sample container 11 is controlled to be opened by rotating the central piece 2, so that the biological sample is put into the central chamber 203, and the operation is convenient.

Preferably, there are two first recesses 201, and the two first recesses 201 are arranged at an angle of 180 degrees on the upper end surface of the central member 2. Correspondingly, two ends of the first protrusion 1102 in the horizontal direction are respectively connected in the two first recesses 201.

In this embodiment, referring to fig. 3 and 14, the lower end of the central member 2 is provided with a second recess 202, and the second recess 202 is used for connecting a second driving member 9 for driving the central member 2 to rotate. Wherein, the second driving member 9 is provided with a second protrusion in fit connection with the second recess 202, and the second driving member 9 and the center member 2 are stably connected through the fit connection of the second protrusion and the second recess 202.

Wherein, the second driving piece 9 includes a rotary spline 901, a magnetic attraction device 902 and a second rotation motor 903, the rotary spline 901 has a second protrusion connected to the central piece 2, the second rotation motor 903 is located on one side of the rotary spline 901 departing from the central piece 2, an output shaft of the second rotation motor 903 passes through the magnetic attraction device 902 to be connected to the rotary spline 901, and the magnetic attraction device 902 can attract the magnetic beads inside the central chamber 203. The output shaft of the second rotating motor 903 rotates to drive the rotating spline 901 to rotate, and the second protrusion on the rotating spline 901 is connected in the second notch 202, so that the rotation of the rotating spline 901 can drive the center piece 2 to rotate, and the relative rotation of the cabin body 1 and the center piece 2 is realized. It can be understood that, when a biological sample is automatically made into an analyte sample, magnetic beads are added to the intermediate product of the analyte sample from the peripheral chamber 103, after the magnetic beads are combined with the intermediate product of the analyte sample to form a magnetic bead sticker, the magnetic bead sticker is separated from the waste generated in the process of forming the magnetic bead sticker, the magnetic attraction device 902 on the second driving member 9 is used to attract the magnetic bead sticker to the inside of the central chamber 203, and the magnetic bead sticker is left in the central chamber 203 under the action of the piston mechanism in the second peripheral chamber, so that the waste generated in the process of forming the magnetic bead sticker is introduced into the corresponding peripheral chamber 103, and the magnetic bead sticker and the waste are smoothly separated, so as to perform the next operation.

Preferably, there are two second recesses 202, two second recesses 202 are arranged on the end surface of the lower end of the central member 2 at an angle of 180 degrees, and each second recess 202 has a corresponding second protrusion. Of course, the number of the second recesses 202 is not limited to two, and the specific number of the second recesses 202 can be flexibly changed as needed in practical design.

Referring to fig. 1, the cylinder 102 is a cylindrical cylinder, the upper end of the cylinder 102 is provided with an air vent 104, and the air vent 104 communicates the peripheral chamber 103 and the mounting hole. The vent 104 can discharge air to the outside of the outer peripheral chamber 103 or suck air into the inner peripheral chamber 103 during the movement of the outer peripheral chamber 103 of the piston mechanism, so as to stabilize the air pressure in the outer peripheral chamber 103 and ensure smooth movement of the piston mechanism in the outer peripheral chamber 103.

In this embodiment, referring to fig. 2, a transparent detection window 106 is disposed on the sidewall of the chamber body 1 corresponding to the reaction chamber, the detection window 106 allows an analyte sample located in the reaction chamber to be detected by an optical signal, and the detector emits an optical signal to detect the analyte sample in the detection window 106. Optical signals include, but are not limited to, fluorescent signals, chemiluminescent signals, electrochemiluminescent signals, colorimetric signals, and the like.

In another embodiment, the outer circumference of the bin body 1 is sleeved with a transparent sleeve, the sleeve provides a protective effect for the bin body 1, and the sleeve is made of a transparent material, so that the detection window 106 is not affected.

Two reaction chambers are arranged in the mounting hole, a cylinder body 102 is arranged between the two reaction chambers, and the two reaction chambers are respectively communicated with a peripheral cavity 103 of the cylinder body 102 positioned between the two reaction chambers through a third communication hole. In this embodiment, the sixth peripheral chamber is respectively communicated with the two reaction chambers. The number of reaction chambers is not limited to two, and in other embodiments, the number of reaction chambers may be one, three, four, or the like.

In an embodiment, referring to fig. 6, a rubber first sealing element 4 is detachably disposed at the lower end of the bin body 1, the first sealing element 4 includes a first body, a first insertion tube 401 is disposed on the first body corresponding to the first through hole 107, a fourth through hole 402 is disposed on the inner sidewall of the first insertion tube 401 corresponding to each first through hole 105, the outer sidewall of the center element 2 abuts against the inner sidewall of the first insertion tube 401 to realize rotational sealing, a second insertion tube 403 is disposed on the first body, the second insertion tube 403 is inserted into the outer circumferential chamber 103, the inner sidewall of the second insertion tube 403 is in sealing fit with the outer sidewall of the piston rod 3 of the piston mechanism, and a fifth through hole communicating with the first through hole 105 is disposed on the second insertion tube 403. The lower end opening of the peripheral chamber 103 is isolated from the external environment by the second cartridge 403, the lower end of the central chamber 203 is isolated from the external environment by the first cartridge 401, and since the first communication hole 105 communicates with the fifth communication hole, a passage is formed between the peripheral chamber 103 and the inside of the central chamber 203, through which fluid communication between the peripheral chamber 103 and the central chamber 203 is possible.

Specifically, referring to fig. 7, a rubber second sealing member 5 is detachably disposed at the upper end of the cartridge body 1, the second sealing member 5 is used for sealing the upper end opening of the peripheral chamber 103, and the upper end of the peripheral chamber 103 is blocked from the external environment by the second sealing member 5.

Referring to fig. 9, the reaction chamber further includes an upper cover 7, the upper cover 7 is disposed on a side of the second sealing member 5 away from the chamber body 1, a second through hole 701 is disposed on the upper cover 7 corresponding to the central cavity 203, and a third notch 702 connected to the sample container 11 is disposed in the second through hole 701. Correspondingly, the upper end of the bottle 1101 of the sample container 11 is provided with a third protrusion 1103 connected with the upper cover 7 of the reaction chamber, after the sample container 11 passes through the second through hole 701, the third protrusion 1103 is connected with the third notch 702 in a matching manner, and the sample container 11 can be supported and limited through the third notch 702.

Specifically, there are two third protrusions 1103, two third protrusions 1103 are disposed on the outer sidewall of the bottle 1101 with an included angle of 180 degrees, there are two third recesses 702, and the third recesses 702 are in one-to-one correspondence with the third protrusions 1103. The sample container 11 is connected to the upper cover 7 by the two third protrusions 1103, which improves the stability of the connection of the sample container 11 to the core member 2.

Referring to fig. 8, the reaction chamber further includes a lower cover 6, the lower cover 6 is disposed on a side surface of the first sealing member 4 away from the chamber body 1, a hole 601 for the central member 2 and the piston mechanism to pass through is disposed on the lower cover 6, and the lower surface of the first body is attached to the upper surface of the lower cover 6. The lower cover 6 mainly serves to provide mechanical protection for the first seal 4. Preferably, the lower cover 6 is integrally injection molded with plastic.

In this embodiment, three heating mechanisms are provided, one heating mechanism for heating one of the peripheral chambers 103, e.g. for heating the elution buffer or lysis buffer inside the peripheral chamber 103, and the remaining two heating mechanisms for heating the analyte sample inside the two reaction chambers.

Wherein, heating mechanism is heat conductor 10, and heat conductor 10 is connected with the heating equipment in storehouse body 1 outside. The heating device provides a heat source for the heat conductor 10, generates heat through the heating device, transfers the heat to the heat conductor 10, and then the heat conductor 10 sends out the heat to heat the reaction chamber or the peripheral cavity 103.

In order to ensure the heating effect of the thermal conductor 10, the thermal conductor 10 is at least partially located in the peripheral chamber 103 or the reaction chamber, so that the thermal conductor 10 is in direct contact with the liquid inside the peripheral chamber 103 or the reaction chamber.

Specifically, referring to fig. 10, the heat conductor 10 is fixedly connected to the lower cover 6, the heat conductor 10 is composed of a first section 1001 and a second section 1002 connected to the first section 1001, the first section 1001 is located above the second section 1002, the second section 1002 is provided with a clamping groove 1003 clamped with the edge of the lower cover 6, and the first section 1001 extends to the outer peripheral chamber 103 or the reaction chamber through the first body. The lower cover 6 is clamped at the edge of the lower cover 6 by the clamping groove 1003 of the heat conductor 10, so that the lower cover 6 provides supporting and fixing acting force for the heat conductor 10.

In this embodiment, the heating mechanisms are provided with three sets, wherein one set of heating mechanism is used for heating the inside of the first peripheral chamber, the remaining two sets of heating mechanisms are respectively used for heating the inside of the two reaction chambers, and the corresponding heating mechanisms of the reaction chambers provide temperatures for the polymerase chain reaction, such as an initial temperature, an annealing temperature, a denaturation temperature, a temperature for extension/elongation, and/or a holding temperature. .

The following is a specific method of use of the reaction chamber:

step S1 is to introduce the biological sample into the sample container 11.

Step S2, inserting the sample container 11 inside the central chamber 203 of the reaction chamber and introducing the corresponding reaction mixture inside the peripheral chamber 103 in the reaction chamber. The sample container 11 effectively prevents contamination of the biological sample by the external environment and transfer of the biological sample to the external environment.

Step S3, placing the reaction bin into a reagent box, driving the bin body 1 and the center piece 2 of the reaction bin to rotate relatively, enabling the central chamber 203 to be communicated with different peripheral chambers 103 in sequence, enabling reaction mixtures corresponding to the insides of the peripheral chambers 103 to react with solutions in the central chamber 203 in sequence according to the sequence of making the analyte samples to obtain first test mixed liquid, and then guiding the obtained first test mixed liquid into the insides of the reaction bins of the reaction bin to react to obtain the analyte samples.

Step S4, the detection window 106 on the reaction chamber detects the analyte sample inside the reaction chamber.

A "biological sample" in the present specification may comprise any sample obtained from a living body or a viral (or prion) source or other biomolecule source, and comprises any cell type or tissue of a subject from which nucleic acids, proteins and/or other biomolecules may be obtained. The biological sample may be a sample obtained directly from a biological source or a processed sample. For example, the amplified isolated nucleic acids constitute a biological sample. Biological samples include, but are not limited to, bodily fluids such as blood, plasma, serum, cerebrospinal fluid, synovial fluid, urine and sweat, tissue and organ samples from animals and plants, and processed samples derived therefrom.

"biological sample" in the present specification means a substance, compound or component whose presence or absence in a sample must be detected.

"reaction mixture" in this specification refers to a solution containing some or all of the necessary reactants to perform a reaction, which may include, but is not limited to, buffers, salts, cofactors, scavengers, and the like, to maintain the pH at a selected level during the reaction.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be appropriately combined to form other embodiments as will be appreciated by those skilled in the art.

The technical principle of the present invention is described above in connection with specific embodiments. The description is made for the purpose of illustrating the principles of the utility model and should not be construed in any way as limiting the scope of the utility model. Based on the explanations herein, those skilled in the art will be able to conceive of other embodiments of the present invention without inventive effort, which would fall within the scope of the present invention.

Claims

1. A reaction chamber, comprising:

2. The reaction chamber according to claim 1, wherein the central member is cylindrical at a position where it fits in the first through hole.

3. The reaction chamber according to claim 1, wherein the center member comprises an upper portion and a lower portion connected to the upper portion, the upper portion having a cross-sectional dimension larger than that of the lower portion, and a step surface is formed between the upper portion and the lower portion, and the step surface abuts against an upper surface of the partition.

4. The reaction chamber according to claim 1, wherein the bottom of the central chamber is disposed obliquely, and the second communication hole communicates with a lowest position of the bottom of the central chamber.

5. The reaction chamber according to claim 4, wherein the second communication hole is inclined downward from an end connecting the central chamber toward an end away from the central chamber.

6. The reaction chamber of claim 1, wherein the central member defines a first recess at an upper end thereof for receiving a sample container.

7. The reaction chamber of claim 1, wherein the lower end of the central member is provided with a second recess for connecting a second driving member for driving the central member to rotate.

8. The reaction chamber according to claim 1, wherein the cylinder is a cylindrical cylinder, and a vent is opened at an upper end of the cylinder, and the vent communicates the peripheral chamber and the mounting hole.

9. The reaction chamber of claim 1, wherein a transparent detection window is disposed on the side wall of the chamber body corresponding to the reaction chamber, and the detection window allows an analyte sample in the reaction chamber to be detected by an optical signal.

10. The reaction chamber according to claim 1, wherein two reaction chambers are provided in the mounting hole, one of the cylindrical bodies is provided between the two reaction chambers, and the two reaction chambers are respectively communicated with the outer peripheral chamber of the cylindrical body therebetween through a third communication hole.

11. The reaction chamber according to claim 1, wherein a rubber first sealing member is detachably disposed at a lower end of the chamber body, the first sealing member comprises a first body, a first insertion cylinder is disposed on the first body corresponding to the first through hole, a fourth communication hole is disposed on an inner sidewall of the first insertion cylinder corresponding to each first through hole, an outer sidewall of the center member abuts against an inner sidewall of the first insertion cylinder to realize rotational sealing, a second insertion cylinder is disposed on the first body, the second insertion cylinder is inserted tightly into the peripheral chamber, an inner sidewall of the second insertion cylinder is in sealing fit with an outer sidewall of a piston rod of the piston mechanism, and a fifth communication hole is disposed on the second insertion cylinder and communicates with the first communication hole.

12. The reaction chamber of claim 11, wherein a rubber second sealing member is detachably disposed at the upper end of the chamber body, and the second sealing member is used for sealing the upper end opening of the peripheral chamber.

13. The reaction chamber of claim 12, further comprising an upper cover, wherein the upper cover is disposed on a side of the second sealing member away from the chamber body, the upper cover is provided with a second through hole corresponding to the central chamber, and the second through hole is provided with a third notch for connecting a sample container.

14. The reaction chamber according to claim 11, further comprising a lower cover, wherein the lower cover is disposed on a side surface of the first sealing member away from the chamber body, the lower cover is provided with a hole for the central member and the piston mechanism to pass through, and the lower surface of the first body is attached to the upper surface of the lower cover.

15. The reaction chamber of claim 14 wherein said heating mechanism is a heat conductor connected to a heating device external to said chamber body.

16. The reaction chamber of claim 15 wherein the thermal conductor is at least partially located within the peripheral chamber or the reaction chamber.

17. The reaction chamber of claim 16, wherein the heat conductor is fixedly connected to the lower cover, the heat conductor comprises a first section and a second section connected to the first section, the first section is located above the second section, the second section has a slot clamped to an edge of the lower cover, and the first section extends through the first body to the peripheral chamber or the reaction chamber.

18. The reaction chamber of claim 11 wherein the piston rod has a piston head at an upper end thereof for sealing the peripheral chamber and a connecting slot at a lower end thereof for connecting to a first drive member for driving the piston mechanism to move.