CN110132695A - A kind of pathological staining system reduction human error is caused to dye abnormal method - Google Patents
A kind of pathological staining system reduction human error is caused to dye abnormal method Download PDFInfo
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- 230000001575 pathological effect Effects 0.000 title claims abstract description 35
- 238000010186 staining Methods 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000002159 abnormal effect Effects 0.000 title claims abstract description 16
- 230000009467 reduction Effects 0.000 title description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 131
- 238000012360 testing method Methods 0.000 claims abstract description 63
- 239000000975 dye Substances 0.000 claims abstract description 11
- 238000012544 monitoring process Methods 0.000 claims description 65
- 239000007788 liquid Substances 0.000 claims description 22
- 230000003595 spectral effect Effects 0.000 claims description 17
- 238000001514 detection method Methods 0.000 claims description 11
- 238000004043 dyeing Methods 0.000 claims description 10
- 230000008439 repair process Effects 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 description 8
- 238000003364 immunohistochemistry Methods 0.000 description 8
- 239000000427 antigen Substances 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 239000000243 solution Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
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- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 1
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
It is reduced the present invention relates to a kind of pathological staining system and dyes abnormal method caused by human error, by before the starting of pathological staining system, first required reagent is detected, if testing result, in normal threshold range, control unit can control the starting of pathological staining system；If testing result have one not in normal threshold range, control unit will not send pathological staining system enabling signal, and corresponding alarm signal is issued, it reminds operator that corresponding reagent is detected or replaced again, avoids because the performance issue of reagent causes coloration result abnormal.
Description
Technical field
The invention belongs to medical detection field, particularly relate to be related to immunohistochemistry and immunocytochemical stain technology
Field more specifically refers to that a kind of full-automatic pathological staining system reduces the method that dyeing is abnormal caused by human error.
Background technique
Immunohistochemistry technology (Immunohistochemistry, IHC) is to combine to exempt from histochemical method
The new technology that the theory and technology of epidemiology grows up, combines the reason of the subjects such as immunology, histology and biochemistry
By and method.Using immunologic core, i.e. the principle that combines of antigen and antibody specificity, with enzyme, metal ion, same is marked
The antibody such as position element track antigen, are shown by chemically reacting to make to be marked on to combine with the developer on the specific antibody on antigen
Certain color, and the result of dyeing is observed by microscope, fluorescence microscope, Electronic Speculum, to reach detection antigen
The purpose of object.
Immunohistochemistry has a long history, and the phase of immunofluorescence technique detection frozen section is established from Coons in 1940
Antigen is answered, has 73 history so far.Nineteen sixty-five, Liu Yanfang etc. establish immunohistochemistry technology at home for the first time, however
Until in the early 1990s, appearance this method of antigen retrieval is just widely used in pathological diagnosis.The technology undergoes 4 development
Stage: the foundation and developing stage of immunohistochemistry technique；Popularize the stage；Clinicopathologic Diagnosis and application stage；Quality control
System, standardization and normalization period.Now, without immunohistochemical auxiliary, many tumours can hardly be made accurately
Diagnosis and classification.Meanwhile extensive use of the immunohistochemistry in Clinicopathologic Diagnosis has also pushed immunohistochemistry technique itself
Fast development.
Key step during immunohistochemical staining includes: roasting piece, dewaxing, aquation, antigen retrieval or peroxidase
Block (property of can choose addition), primary antibody, secondary antibody or three anti-(detection systems), DAB or haematoxylin (alternative addition) and institute
There is flushing.
With the development of science and technology, various advanced technologies are also gradually applied in the automation dyeing course of immunohistochemistry, with
Substitute hand dyeing.In order to overcome the difference of artificially colored result, immunohistochemistry automation coloring system is developed, and
And in recent years, the automatic clinical chemistry analyzer of various different models and function emerges in large numbers in succession, greatly improves working efficiency
With the accuracy of inspection.
But in full-automatic pathological staining system operation, in different processes, need using different reagents,
Influencing full-automatic pathological staining systematic dyeings regular element is mainly three, and first is histotomy factor, and second is
System factor itself, third are reagent factor.Wherein, first factor and second factor can be guaranteed by human intervention
Coloration result it is normal, still, used reagent, because type is more, and the nature difference of each reagent is larger, if carry out
Heavy workload required for constantly detecting, and time-consuming and at high cost.
Summary of the invention
It is reduced the object of the present invention is to provide a kind of pathological staining system and dyes abnormal method caused by human error, to solve
The prior art cannot immediately to it is used it is agents can be carried out detection, and lead to the problem of coloration result exception.
The present invention is achieved by the following technical solutions:
A kind of pathological staining system reduction human error is caused to dye abnormal method, comprising the following steps:
Determine corresponding normal range (NR) threshold value respectively according to type for reagent required for dyeing course, and by it is above-mentioned just
Normal range threshold storage；
The control unit of pathological staining system receives the wake-up signal of activation system, and described control unit is to on-line monitoring
Control unit sends enabling signal；
After the on-line monitoring unit receives enabling signal, the on-line monitoring unit controls corresponding inductor and opens
Dynamic, the testing result information of the reagent corresponding to it is passed to the on-line monitoring unit by the corresponding inductor；
The testing result information that the on-line monitoring unit will receive is compared with the threshold value of the reagent of corresponding type,
And judge:
If testing result information, in the threshold range of the reagent of corresponding type, the on-line monitoring unit is to the control
Unit feeds back normal signal, and described control unit controls the starting of pathological staining system；
If testing result information, not in the threshold range of the reagent of corresponding type, the on-line monitoring unit is to the control
Unit processed feeds back spectral artifacts, and described control unit does not start pathological staining system, concurrently after receiving spectral artifacts
Send caution signal.
The testing result information that the on-line monitoring unit will receive is compared with the threshold value of the reagent of corresponding type,
The following steps are included:
Determine the type N of reagent, wherein N is natural number；
1) threshold value of the testing result information of on-line monitoring the first reagent of unit judges and the first reagent；If the
In the threshold range of the first reagent, the on-line monitoring unit carries out next step for a kind of testing result information of reagent；
If the testing result information of the first reagent is not in the threshold range of the first reagent, the on-line monitoring unit
Spectral artifacts are fed back to control unit；
2) threshold value of the testing result information of described on-line monitoring second of reagent of unit judges and second of reagent；
If the testing result information of second of reagent in the threshold range of second of reagent, the on-line monitoring unit into
Row next step；
If the testing result information of second of reagent is not in the threshold range of second of reagent, the on-line monitoring unit
Spectral artifacts are fed back to control unit；
3) until the threshold value of the testing result information of the on-line monitoring unit judges N kind reagent and N kind reagent；
If the testing result information of N kind reagent is in the threshold range of N kind reagent, the on-line monitoring unit feedback
Normal signal is to control unit, described control unit control control pathological staining system starting；
If the testing result information of N kind reagent, not in the threshold range of N kind reagent, the on-line monitoring unit is anti-
Spectral artifacts are presented to control unit.
The testing result information that the on-line monitoring unit will receive is compared with the threshold value of the reagent of corresponding type,
The following steps are included:
Determine the type N of reagent, wherein N is natural number；
It is described on-line monitoring unit receive N kind reagent testing result information, and respectively with the threshold value of corresponding reagent into
Row compares, and judges:
If the testing result information of the N kind reagent is respectively in corresponding reagent threshold range, the on-line monitoring
Unit feeds back normal signal to described control unit；
If having a kind of testing result information of reagent not in corresponding reagent threshold range, the on-line monitoring unit
Spectral artifacts are fed back to described control unit.
Every kind of reagent testing result information includes pH value result information, and the threshold of the pH value of every kind of reagent
It is identical or different to be worth range.
The reagent includes a variety of detection auxiliary reagents, the threshold range of the pH value of the detection auxiliary reagent are as follows:
(1) acid liquid pH value range of repairing: 6.0 ± 0.2；
(2) neutral liquid pH value range of repairing: 7.4 ± 0.2；
(3) alkalinity reparation liquid pH value range: 8.0 ± 0.2 to 9.0 ± 0.2；
(4) PBS buffer solution pH value range: 7.4 ± 0.2；
(5) distilled water pH value range: 7.0 ± 0.5.
Every kind of reagent testing result information includes total dissolved solidss total amount TDS result information, and described in every kind
The threshold range of the TDS value of reagent is identical or different.
The threshold range of the TDS value are as follows:
(1) acid to repair liquid TDS < 1000；
(2) neutral to repair liquid TDS < 500；
(3) alkalinity repairs liquid TDS < 200；
(4) PBS buffer solution TDS < 6500；
(5) distilled water TDS < 50.
The beneficial effects of the present invention are:
The technical program is by first detecting required reagent before the starting of pathological staining system, if detection knot
For fruit in normal threshold range, control unit can control the starting of pathological staining system；If testing result have one not normal
Threshold range in, control unit will not send pathological staining system enabling signal, and issue corresponding alarm signal, remind behaviour
Make personnel and corresponding reagent is detected or replaced again, avoids because the performance issue of reagent causes coloration result abnormal.
Detailed description of the invention
Fig. 1 is that pathological staining system of the present invention starts control logic figure.
Specific embodiment
Carry out the technical solution that the present invention will be described in detail by the following examples, embodiment below is merely exemplary, only
It can be used to explanation and illustration technical solution of the present invention, and be not to be construed as the limitation to technical solution of the present invention.
The application provides a kind of caused dyeing of pathological staining system reduction human error abnormal method, as shown in Figure 1, including
Following steps:
Determine corresponding normal range (NR) threshold value respectively according to type for reagent required for dyeing course, and by it is above-mentioned just
Normal range threshold storage, specific storage location are possibly stored in the memory module of control unit, are also possibly stored to supervise online
It surveys in the memory module of unit, is stored in individual memory module.
Here, being set as N class herein for reagent required for dyeing overall process, wherein N is natural number, and corresponding is N
A threshold range, and threshold range and reagent correspond.
The control unit of pathological staining system receives the wake-up signal of activation system, and control unit is to Online Monitoring Control
Unit sends enabling signal.In the present embodiment, the wake-up signal of activation system can be by being set to pathological staining System Computer
Switch on body is realized, can also wake up control unit by wireless signal, as the interaction of wireless signal and control unit
Mode can be thus achieved using the wireless interaction mode of the prior art, herein without detailed description.
After control unit receives wake-up signal, control unit can also include upper before waking up on-line monitoring unit
Electric control first controls pathological staining system electrification, into the preproduction phase of starting, it is also possible to after by powering on, online
The starting of monitoring unit control sensor.It in the present embodiment, is on-line monitoring after waking up on-line monitoring unit by control unit
Unit control sensor powers on and enters working condition.
In this application, inductor is at least N number of, and wherein N is natural number, can be realized a pair of with reagent type at least one
It answers, certainly, because the detection project of every kind of reagent can be one or more, the quantity of inductor can also increase accordingly
Add.In the other embodiments of the application, integrated inductor can also be used, for example, the pH value of acquisition all kinds reagent,
Realize that the different channels transmission of same information reduces pathology to reduce the quantity of inductor by an integrated inductor
The volume of coloring system or the quantity of route.
The testing result information of the corresponding reagent of each inductor after actuation, can be passed to on-line monitoring by inductor
Unit.
The testing result information received is compared by on-line monitoring unit with the threshold value of the reagent of corresponding type, and is sentenced
It is disconnected:
In this application, including two kinds of judgment modes:
The first are as follows:
Determine the type N of reagent, wherein N is natural number；
1) the testing result information of the first reagent of unit judges and the threshold value of the first reagent are monitored on-line；If the first
For the testing result information of reagent in the threshold range of the first reagent, on-line monitoring unit carries out next step；
If the testing result information of the first reagent is not in the threshold range of the first reagent, on-line monitoring unit feedback
Spectral artifacts are to control unit；
2) the testing result information of second of reagent of unit judges and the threshold value of second of reagent are monitored on-line；
If the testing result information of second of reagent, in the threshold range of second of reagent, on-line monitoring unit carries out down
One step；
If the testing result information of second of reagent is not in the threshold range of second of reagent, on-line monitoring unit feedback
Spectral artifacts are to control unit；
3) until the threshold value of the testing result information of on-line monitoring unit judges N kind reagent and N kind reagent；
If the testing result information of the reagent, in the threshold range of N kind reagent, on-line monitoring unit feedback is normal
Signal is to control unit, control unit control control pathological staining system starting；
If the testing result information of N kind reagent, not in the threshold range of N kind reagent, on-line monitoring unit feedback is non-
Normal signal is to control unit.
Second:
Determine the type N of reagent, wherein N is natural number；
It monitors unit on-line and receives the testing result information of N kind reagent, and compared respectively with the threshold value of corresponding reagent
Compared with, and judge:
If the testing result information of N kind reagent is respectively in corresponding reagent threshold range, on-line monitoring unit feedback
Normal signal is to control unit；
If there is a kind of testing result information of reagent not in corresponding reagent threshold range, on-line monitoring unit feedback
Spectral artifacts are to control unit.
As for which kind of above-mentioned judgment method selected, the control method of the application can be realized.
It is illustrated by taking the pH value of reagent as an example herein, includes pH usually in the testing result information of every kind of reagent
It is worth result information, and the threshold range of the pH value of every kind of reagent is identical or different.
It is of course also possible to not include pH value result information in certain or the testing result information of certain reagents.
For example, a certain alkalinity repairs liquid, in the present embodiment, the threshold range for repairing the pH value of liquid is 9.0 ± 0.2.
In to the testing result information for repairing liquid, if detecting pH value are as follows: 8.8 < pH value < 9.2 illustrate the reparation liquid
Result information in its threshold value, at this point, on-line monitoring unit carries out sentencing for next step if according to the first judgment mode
It is disconnected.
If detecting pH value are as follows: pH < 8.8 or pH > 9.2, illustrate the result information of the reparation liquid not in its threshold value, this
When, on-line monitoring unit directly feeds back spectral artifacts to control unit, and sending control system enabling signal is not same for control unit
When send alarm signal, to remind operator to check or Replacement and Repair liquid.
Such as the threshold range < 50 of the TDS value of distilled water.In to the testing result information for repairing liquid, TDS is detected
Value is 100, illustrates the result information of the reparation liquid in its threshold value, at this point, if on-line monitoring is single according to the first judgment mode
Member carries out the judgement of next step.
If detecting, TDS value is 10, illustrates the result information of the reparation liquid not in its threshold value, at this point, on-line monitoring is single
Member directly feedback spectral artifacts are to control unit, and sending control system enabling signal does not send alarm signal to control unit simultaneously
Number, to remind operator to check or Replacement and Repair liquid.
According to second of judgment mode, the testing result information of all reagents is passed to on-line monitoring by all inductors
Unit, on-line monitoring unit is in carrying out deterministic process, if there is a kind of testing result information of reagent not in corresponding threshold value
Within, on-line monitoring unit directly interrupts judgement, and feeds back spectral artifacts to control unit, while sending alarm signal, with
Operator is reminded to check or replace corresponding reagent.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And deformation, the scope of the present invention are extremely equally limited by appended claims.
Claims (7)
1. a kind of pathological staining system, which reduces, dyes abnormal method caused by human error, which comprises the following steps:
Corresponding normal range (NR) threshold value is determined respectively according to type for reagent required for dyeing course, and by above-mentioned normal model
Enclose threshold value storage；
The control unit of pathological staining system receives the wake-up signal of activation system, and described control unit is to Online Monitoring Control
Unit sends enabling signal；
After the on-line monitoring unit receives enabling signal, the on-line monitoring unit controls corresponding inductor starting, phase
The testing result information of reagent corresponding to it is passed to the on-line monitoring unit by the inductor answered；
The testing result information that the on-line monitoring unit will receive is compared with the threshold value of the reagent of corresponding type, and is sentenced
It is disconnected:
If testing result information, in the threshold range of the reagent of corresponding type, the on-line monitoring unit is to described control unit
Normal signal is fed back, described control unit controls the starting of pathological staining system；
If testing result information is not in the threshold range of the reagent of corresponding type, the on-line monitoring unit is single to the control
Member feedback spectral artifacts, described control unit does not start pathological staining system after receiving spectral artifacts, and sends police
Accuse signal.
2. pathological staining system according to claim 1, which reduces, dyes abnormal method caused by human error, which is characterized in that
The testing result information that the on-line monitoring unit will receive is compared with the threshold value of the reagent of corresponding type, including following
Step:
Determine the type N of reagent, wherein N is natural number；
1) threshold value of the testing result information of on-line monitoring the first reagent of unit judges and the first reagent；If the first
For the testing result information of reagent in the threshold range of the first reagent, the on-line monitoring unit carries out next step；
If the testing result information of the first reagent is not in the threshold range of the first reagent, the on-line monitoring unit feedback
Spectral artifacts are to control unit；
2) threshold value of the testing result information of described on-line monitoring second of reagent of unit judges and second of reagent；
If the testing result information of second of reagent, in the threshold range of second of reagent, the on-line monitoring unit carries out down
One step；
If the testing result information of second of reagent is not in the threshold range of second of reagent, the on-line monitoring unit feedback
Spectral artifacts are to control unit；
3) until the threshold value of the testing result information of the on-line monitoring unit judges N kind reagent and N kind reagent；
If the testing result information of N kind reagent, in the threshold range of N kind reagent, the on-line monitoring unit feedback is normal
Signal is to control unit, described control unit control control pathological staining system starting；
If the testing result information of N kind reagent, not in the threshold range of N kind reagent, the on-line monitoring unit feedback is non-
Normal signal is to control unit.
3. pathological staining system according to claim 1, which reduces, dyes abnormal method caused by human error, which is characterized in that
The testing result information that the on-line monitoring unit will receive is compared with the threshold value of the reagent of corresponding type, including following
Step:
Determine the type N of reagent, wherein N is natural number；
The on-line monitoring unit receives the testing result information of N kind reagent, and is compared respectively with the threshold value of corresponding reagent
Compared with, and judge:
If the testing result information of the N kind reagent is respectively in corresponding reagent threshold range, the on-line monitoring unit
Normal signal is fed back to described control unit；
If there is a kind of testing result information of reagent not in corresponding reagent threshold range, the on-line monitoring unit feedback
Spectral artifacts are to described control unit.
4. pathological staining system according to any one of claim 1 to 3, which reduces, dyes abnormal method caused by human error,
It is characterized in that, every kind of reagent testing result information includes pH value result information, and the pH value of every kind of reagent
Threshold range it is identical or different.
5. pathological staining system according to claim 4, which reduces, dyes abnormal method caused by human error, which is characterized in that
The reagent includes a variety of detection auxiliary reagents, the threshold range of the pH value of the detection auxiliary reagent are as follows:
(1) acid liquid pH value range of repairing: 6.0 ± 0.2；
(2) neutral liquid pH value range of repairing: 7.4 ± 0.2；
(3) alkalinity reparation liquid pH value range: 8.0 ± 0.2 to 9.0 ± 0.2；
(4) PBS buffer solution pH value range: 7.4 ± 0.2；
(5) distilled water pH value range: 7.0 ± 0.5.
6. pathological staining system according to any one of claim 1 to 3, which reduces, dyes abnormal method caused by human error,
It is characterized in that, every kind of reagent testing result information includes TDS value result information, and the TDS of every kind of reagent
The threshold range of value is identical or different.
7. pathological staining system according to claim 6, which reduces, dyes abnormal method caused by human error, which is characterized in that
The threshold range of the TDS value are as follows:
(1) acid to repair liquid TDS < 1000；
(2) neutral to repair liquid TDS < 500；
(3) alkalinity repairs liquid TDS < 200；
(4) PBS buffer solution TDS < 6500；
(5) distilled water TDS < 50.
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Address after: Building 12, innovation park, Haixi high tech Industrial Park, Fuzhou high tech Zone, No. 3, Keji East Road, Minhou County, Fuzhou City, Fujian Province Patentee after: FUZHOU MAIXIN BIOTECH, Co.,Ltd. Patentee after: Lumingte biological system Co., Ltd Address before: 350000 building 12, innovation park, Haixi high tech Industrial Park, Fuzhou high tech Zone, No. 3, Keji East Road, Minhou County, Fuzhou City, Fujian Province Patentee before: FUZHOU MAIXIN BIOTECH, Co.,Ltd. Patentee before: Lutes biosystems |