CN118020801A

CN118020801A - Traditional Chinese medicine spray for sterilizing animal breeding environment

Info

Publication number: CN118020801A
Application number: CN202410104105.5A
Authority: CN
Inventors: 朱瑞娟; 杨文文; 郎元君; 杨瑞锋; 魏培; 王娟弟; 李得鑫; 张庆莉; 闫绍悦; 王成森; 孙新堂; 吕娜娜
Original assignee: Shandong Vocational Animal Science and Veterinary College
Current assignee: Shandong Vocational Animal Science and Veterinary College
Priority date: 2024-01-25
Filing date: 2024-01-25
Publication date: 2024-05-14

Abstract

The invention discloses a traditional Chinese medicine spray for sterilizing animal breeding environments, and belongs to the technical field of air disinfectants. The traditional Chinese medicine spray disclosed by the invention is prepared from the following components in parts by weight: 50-80 parts of rhizoma atractylodis, 10-30 parts of magnolia officinalis, 10-30 parts of fructus forsythiae, 10-30 parts of honeysuckle, 20-50 parts of radix scutellariae, 50-80 parts of folium artemisiae argyi, 20-60 parts of rhizoma acori graminei, 10-30 parts of mint and 10-30 parts of clove. The disinfectant prepared by the invention contains the traditional Chinese medicine volatile oil, can purify air, eliminate peculiar smell, has remarkable disinfection and sterilization effects, is natural, nontoxic, nonirritant, residue-free and drug-resistant, and is widely applicable to disinfection of animal cultivation environments.

Description

Traditional Chinese medicine spray for sterilizing animal breeding environment

Technical Field

The invention belongs to the technical field of air disinfectants, and particularly relates to a traditional Chinese medicine spray for disinfecting animal breeding environments.

Background

In recent years, the prevalence of infectious diseases such as avian influenza, african swine fever, brucellosis, eastern coastal fever, african animal trypanosomiasis, newcastle disease, porcine Reproductive and Respiratory Syndrome (PRRS) and foot-and-mouth disease has increased increasingly, and the necessary disinfection of animal breeding environments can effectively avoid the spread of diseases. At present, the sterilization of animal breeding environment mainly comprises a physical sterilization method and a chemical sterilization method, wherein the physical sterilization method comprises mechanical cleaning and sunlight or ultraviolet sterilization. The mechanical cleaning is to clean the animal farm by a mechanical method to thoroughly remove the dehairing, dirt, excrement and the like. Sunlight or ultraviolet ray sterilization is to expose daily necessities of animals to the sun or to radiate the daily necessities by ultraviolet rays to kill pathogenic microorganisms, parasite eggs, fungi and the like on the surfaces of the daily necessities. The common disinfectants used in the chemical disinfection method include sodium hydroxide, peracetic acid, bleaching powder, formaldehyde, quicklime, organophosphorus pesticides and the like, and can disinfect floors, walls, nursing tools and the like. Both physical and chemical sterilization methods have some sterilizing efficacy, but remain limited. The mechanical cleaning can not realize effective sterilization, the ultraviolet rays can only sterilize the surface of an object directly irradiated, and the mechanical cleaning has the defects of incomplete sterilization, cell killing effect and short time effect; although the chemical disinfectant has strong sterilization capability and can kill bacteria, fungi, viruses and the like, the chemical disinfectant has strong irritation and even toxic components, is only suitable for environment and objects, can not be used for sterilizing the environment where people are present, and has limited use. At present, traditional Chinese medicine disinfection products which are green, natural, high in safety coefficient and strong in sterilization effect and are used for disinfecting the environment of the pet and livestock and poultry raising places are increasingly valued by scientific researchers and favored by markets.

Disclosure of Invention

Aiming at the problems existing in the prior art, the invention aims to provide a traditional Chinese medicine spray for sterilizing animal breeding environments.

In order to achieve the above purpose, the invention adopts the following technical scheme:

A traditional Chinese medicine composition comprises the following components in parts by weight:

50-80 parts of rhizoma atractylodis, 10-30 parts of magnolia officinalis, 10-30 parts of fructus forsythiae, 10-30 parts of honeysuckle, 20-50 parts of radix scutellariae, 50-80 parts of folium artemisiae argyi, 20-60 parts of rhizoma acori graminei, 10-30 parts of mint and 10-30 parts of clove.

The application of the traditional Chinese medicine composition in preparing an animal breeding environment disinfection reagent.

A traditional Chinese medicine spray for disinfecting animal breeding environments is prepared from the following components in parts by weight:

In a specific embodiment, the traditional Chinese medicine spray for disinfecting animal raising environments is prepared from the following components in parts by weight:

60 parts of rhizoma atractylodis, 20 parts of magnolia officinalis, 20 parts of fructus forsythiae, 20 parts of honeysuckle, 40 parts of radix scutellariae, 60 parts of folium artemisiae argyi, 40 parts of rhizoma acori graminei, 20 parts of mint and 20 parts of clove.

The preparation method of the traditional Chinese medicine spray for disinfecting animal breeding environment comprises the following steps:

(1) Extracting rhizoma Atractylodis and cortex Magnolia officinalis with supercritical CO ₂ fluid to obtain extract A;

(2) Decocting fructus forsythiae, flos Lonicerae, and Scutellariae radix with water to obtain concentrated solution B, C, D;

(3) Extracting volatile oil from folium Artemisiae Argyi, rhizoma Acori Graminei, herba Menthae, and flos Caryophylli, respectively to obtain volatile oil E, F, G, H;

(4) Mixing extract A, concentrated solution B, C, D and volatile oil E, F, G, H, adding 5% wt isopropyl alcohol, and diluting with purified water to obtain solution containing 0.05g crude drug per mL.

In a specific embodiment, the conditions for supercritical CO ₂ fluid extraction in step (1) are: the extraction pressure is 25MPa, the extraction temperature is 55 ℃, the resolution I pressure is 7.5MPa, the resolution I temperature is 34 ℃, and the extraction time is 2h.

In a specific embodiment, the method for extracting fructus forsythiae in the step (2) includes: decocting with 8 times of water for 3 times each for 1.5 hours, filtering, mixing filtrates, concentrating the filtrate under reduced pressure below 60deg.C to obtain fluid extract with relative density of 1.10-1.20, cooling, adding 4 times of ethanol, stirring, standing for 2 hours, filtering, recovering ethanol from the filtrate under reduced pressure, concentrating the concentrate to 1L to obtain concentrate B;

The honeysuckle extraction method in the step (2) comprises the following steps: soaking in 10 times of water for 30min, decocting for 3 times each for 1.5 hr, filtering, mixing filtrates, and concentrating the filtrate to 1L to obtain concentrated solution C;

the method for extracting the scutellaria baicalensis in the step (2) comprises the following steps: soaking in 10 times of water for 1 hr, decocting for 3 times each for 1 hr, mixing decoctions, and concentrating to 1L to obtain concentrated solution D.

In a specific embodiment, the extracting method of the volatile oil of the mugwort leaf, the grassleaved sweetflag rhizome, the peppermint and the clove in the step (3) comprises the following steps: soaking folium Artemisiae Argyi, rhizoma Acori Graminei, herba Menthae, and flos Caryophylli in water for 2-3 hr, extracting in volatile oil extractor for 6 hr, standing, collecting volatile oil, and drying with anhydrous sodium sulfate.

The traditional Chinese medicine spray prepared by the method is applied to disinfection of animal breeding environments.

A method for sterilizing animal breeding environment comprises spraying and sterilizing animal breeding environment with Chinese medicinal spray prepared by the method according to usage amount of 5mL/m ³.

The technical scheme of the invention has the advantages that:

Compared with the prior art, the invention takes the pungent and bitter herbs including rhizoma atractylodis, folium artemisiae argyi, clove and rhizoma acori graminei as main materials and takes the bitter and cold herbs including radix scutellariae, peppermint, fructus forsythiae, honeysuckle and magnolia officinalis as auxiliary materials, and the medicines are extracted by the optimal process and then are combined, so that the synergistic effect can be effectively achieved. The disinfectant contains the volatile oil of the traditional Chinese medicine, can purify air, eliminate peculiar smell, has remarkable disinfection and sterilization effects, is natural, nontoxic, nonirritant, residue-free and drug-resistant, and is widely applicable to disinfection of animal cultivation environments.

Pharmacological actions and effects of the traditional Chinese medicines:

Rhizoma atractylodis: is dried rhizome of Atractylodes lancea Atractylodes lancea (thunder.) DC or Atractylodes lancea Atractylodes chinensis (DC.) Koidz. Pungent and bitter taste, warmth, spleen, stomach and liver meridians entered, and has the effects of drying dampness and strengthening spleen, dispelling wind and cold and improving eyesight. In the recorded plague avoidance prescription, the frequency of use of rhizoma atractylodis is first. As early as the Han dynasty, the Sheng Zhang Zhong Jing said that "Zhu can avoid all bad smell" and Ming Su Liu Zhu in Ben Cao gang mu "also recorded the popular style of using Zhu to burn smoke to avoid pathogenic smell when epidemic or denier. Modern researches have shown that the main ingredients of rhizoma Atractylodis are eneyne (such as atractylin), sesquiterpenes (such as beta-eucalyptol, hinokitiol, and atractylenolide), saccharides, sterols, etc. The volatile oil components such as atractylin, beta-eucalyptol and the like are main medicinal components, have the effects of inactivating and inhibiting escherichia coli, salmonella typhi, staphylococcus aureus, pseudomonas aeruginosa and the like, and the volatile oil spray, fumigation solution and fumigant of the atractylis can be used for sterilizing air and environment.

Mugwort leaf: is a dry leaf of Artemisia argyi ARTEMISIA ARGYI Levi et vant of Compositae, has pungent and bitter taste, is warm, enters liver, spleen and kidney meridians, and has effects of warming meridians to stop bleeding, dispelling cold and relieving pain. Researches show that the chemical components of the Chinese medicinal composition mainly comprise volatile oil, triterpenes, polysaccharides, flavonoids and the like, and have various pharmacological effects of resisting bacteria, relieving cough, eliminating phlegm, resisting oxidation, resisting inflammation and the like. The volatile oil of folium Artemisiae Argyi has effects of killing or inhibiting Staphylococcus aureus, beta-hemolytic streptococcus, tubercle bacillus, typhoid bacillus, paratyphoid bacillus, diphtheria bacillus, and colibacillus.

Clove: is a dried bud of Eugenia caryophyllata Eugenia caryophyllata th of Myrtaceae, has pungent taste, is warm, enters spleen, stomach, lung and kidney meridians, and has effects of warming middle-jiao, lowering adverse qi, invigorating kidney and supporting yang. Research shows that the chemical components of the medicine mainly comprise volatile oil, flavonoids, terpenes, sterols and the like. The main component eugenol in the volatile oil has inhibiting effect on gram positive bacteria (such as Staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, etc.) and gram negative bacteria (such as Escherichia coli, salmonella typhi, pseudomonas aeruginosa, etc.). Eugenol has a good inhibition effect on drug-resistant strains, and can inhibit the expression of methicillin-resistant staphylococcus aureus (MRSA) biofilm formation key genes (IcaA, icaD and SarA); vancomycin resistant staphylococcus aureus strains are destroyed by stimulating the production of Reactive Oxygen Species (ROS) and membrane damage in the cells.

Rhizoma Acori Graminei: is dried rhizome of rhizoma Acori Graminei Acorus tatarinowii Schott belonging to Araceae, and has pungent and bitter taste, warm taste, and heart and stomach meridians. Has effects of inducing resuscitation, eliminating phlegm, eliminating dampness, and regulating stomach function. Research shows that the chemical components of the water-soluble organic compound mainly comprise volatile oil, organic acid, sugar, amino acid and the like. The rhizoma acori graminei volatile oil has different degrees of inhibition effects on staphylococcus epidermidis, group A streptococcus, apple anthracnose pathogen, walnut canker pathogen and the like.

Radix Scutellariae Baicalensis: is dry root of Scutellaria baicalensis Georgi Scutelaria baicalensis Georgi belonging to Labiatae, bitter in taste, cold in nature, and enters lung, gallbladder, spleen, large intestine and small intestine meridians. Has effects of clearing heat, eliminating dampness, purging pathogenic fire, removing toxic substances, stopping bleeding, and preventing miscarriage. Researches show that the chemical components of the composition mainly comprise baicalin, baicalein, wogonin, 5, 7-dihydroxyl-6-methoxyl dihydroflavone and the like, have broad-spectrum antibacterial effect, and have good inhibition effect on gram-positive bacteria (staphylococcus aureus, hemolytic streptococcus, pneumococcus and the like) and gram-negative bacteria (escherichia coli, bacillus dysenteriae, pseudomonas aeruginosa and the like).

Peppermint: is a dry part of the plant Mentha haplocalyx Mentha haplocalyx Briq. Of Labiatae, has pungent taste, cool nature, and has effects of dispelling pathogenic wind, clearing heat, refreshing, relieving sore throat, and promoting eruption. Researches show that the main active ingredient of the mint is mint oil, and has obvious antibacterial activity on escherichia coli, staphylococcus aureus and candida albicans.

Fructus forsythiae: is a dried fruit of Forsythia Forsythiasuspensa (thunder.) Vahl belonging to Oleaceae. Bitter in taste, slightly cold in nature, enters lung, heart and small intestine meridians, and has the effects of clearing heat and detoxicating, detumescence and resolving masses and dispelling wind and heat. Fructus forsythiae has effects of clearing heat and detoxicating, and is called "sores' holy medicine". Researches show that the main chemical components of the composition comprise lignans, flavonoids, volatile components, phenethyl compounds, ethylcyclohexyls, triterpenes, coumarins and the like. The concentrated decoction of fructus forsythiae has antibacterial effect in vitro, and can inhibit Staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, typhoid bacillus, paratyphoid bacillus, dysentery bacillus, diphtheria bacillus, vibrio cholerae, streptococcus, etc.

Honeysuckle flower: is prepared from dried flower bud or flower with primary opening of Lonicera japonica Thunb Lonicera japonica Thunb of Caprifoliaceae, and has effects of clearing heat, removing toxic substances, dispelling pathogenic wind and heat. Honeysuckle has the reputation of "traditional Chinese medicine antibiotics". Researches show that the main components of the composition are organic acids, flavonoids, volatile oil and iridoid compounds. The honeysuckle extract has remarkable inhibition effect on white staphylococcus aureus, streptococcus A, streptococcus B and gram positive bacteria, and meanwhile, the honeysuckle also has inhibition effect on various pathogenic bacteria including escherichia coli, and the antibacterial effect is stronger than that of amoxicillin, florfenicol and other chemicals, and the side effect is small.

Cortex Magnoliae officinalis: is dried bark, root bark, and branch bark of Magnoliaceae plant Magnolia officinalis Magnolia officinalis Red. Et Wils. Or Magnolia officinalis Magnolia officinalis Red. Et Wils. Var. Bioloba Red. Et Wils. Bitter and pungent taste, warm nature, and spleen, stomach, lung and large intestine meridians entered, it has the actions of drying dampness and resolving phlegm, descending qi and removing fullness. Researches show that the magnolia officinalis decoction has inhibiting effects on gram-positive bacteria such as staphylococcus, hemolytic streptococcus, pneumococcus and pertussis bacillus, and gram-negative bacteria such as bacillus anthracis, bacillus dysenteriae, typhoid bacillus, paratyphoid bacillus, vibrio cholerae, escherichia coli, bacillus proteus, bacillus subtilis and diphtheria bacillus.

Detailed Description

The terms used in the present invention generally have meanings commonly understood by those of ordinary skill in the art unless otherwise indicated.

The invention will be described in further detail below in connection with specific embodiments and with reference to the data. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.

The following is adopted:

PBS is phosphate buffer: concentration 0.03moL/L, pH 7.2;

Staphylococcus aureus (ATCC 6538), escherichia coli (8099), candida albicans (ATCC 10231), all provided by the chinese pharmaceutical biologicals assay;

healthy mice of Kunming species, japanese white rabbits, were all supplied by the Shandong animal husbandry veterinary professional college animal laboratory center.

Example 1

50 parts of rhizoma atractylodis, 10 parts of magnolia officinalis, 10 parts of fructus forsythiae, 10 parts of honeysuckle, 20 parts of radix scutellariae, 50 parts of folium artemisiae argyi, 20 parts of rhizoma acori graminei, 10 parts of mint and 10 parts of clove.

The preparation method comprises the following steps:

(1) Pulverizing dried rhizoma Atractylodis and cortex Magnolia officinalis into 10 mesh powder, and extracting with supercritical CO ₂ fluid to obtain extract A.

The extraction conditions are as follows: extracting at 55deg.C under 25MPa at 7.5MPa and 34 deg.C for 2 hr;

(2) Decocting dried fructus forsythiae, flos Lonicerae, and Scutellariae radix with water respectively to obtain concentrated solutions B, C, D.

The fructus forsythiae extraction method comprises the following steps: decocting with 8 times of water for 3 times each for 1.5 hours, filtering, mixing filtrates, concentrating the filtrate under reduced pressure below 60deg.C to obtain fluid extract with relative density of 1.10-1.20 (room temperature), cooling, adding 4 times of ethanol, stirring, standing for 2 hours, filtering, recovering ethanol from the filtrate under reduced pressure, concentrating the concentrate to 1L to obtain concentrate B;

The honeysuckle extraction method comprises the following steps: soaking in 10 times of water for 30min, decocting for 3 times each for 1.5 hr, filtering, mixing filtrates, and concentrating the filtrate to 1L to obtain concentrated solution C;

The extraction method of the baical skullcap root comprises the following steps: soaking in 10 times of water for 1 hr, decocting for 3 times each for 1 hr, mixing decoctions, and concentrating to 1L to obtain concentrated solution D.

(3) Extracting volatile oil from dried folium Artemisiae Argyi, rhizoma Acori Graminei, herba Menthae, and flos Caryophylli, respectively, to obtain volatile oil E, F, G, H.

The mugwort leaf volatile oil, the grassleaved sweetflag rhizome volatile oil, the peppermint volatile oil and the clove volatile oil can be commercially available volatile oil or can be prepared by the following method:

The extraction method of the mugwort leaf volatile oil comprises the following steps: soaking in 10 times of water for 2 hr, extracting in volatile oil extractor for 6 hr, standing for a moment, collecting volatile oil, and drying with anhydrous sodium sulfate;

The extraction method of the grassleaf sweelflag rhizome volatile oil comprises the following steps: soaking in 8 times of water for 2.5 hr, extracting in volatile oil extractor for 6 hr, standing for a moment, collecting volatile oil, and drying with anhydrous sodium sulfate;

The extraction method of the peppermint volatile oil comprises the following steps: soaking in 10 times of water for 2 hr, ultrasonic treating for 30min, extracting in volatile oil extractor for 3 hr, standing for a moment, collecting volatile oil, and drying with anhydrous sodium sulfate;

The extraction method of the clove volatile oil comprises the following steps: soaking in 11 times of water for 3 hr, extracting in volatile oil extractor for 5 hr, standing for a moment, collecting volatile oil, and drying with anhydrous sodium sulfate;

(4) Mixing extract A, concentrated solution B, C, D and volatile oil E, F, G, H, adding 5% wt isopropanol, diluting with purified water to obtain solution containing 0.05g crude drug per mL, and packaging into 250mL spray bottle.

Example 2

55 parts of rhizoma atractylodis, 15 parts of magnolia officinalis, 15 parts of fructus forsythiae, 15 parts of honeysuckle, 25 parts of radix scutellariae, 55 parts of folium artemisiae argyi, 25 parts of rhizoma acori graminei, 15 parts of mint and 15 parts of clove.

The preparation method is the same as in example 1.

Example 3

The preparation method is the same as in example 1.

Example 4

65 parts of rhizoma atractylodis, 25 parts of magnolia officinalis, 25 parts of fructus forsythiae, 25 parts of honeysuckle, 45 parts of radix scutellariae, 65 parts of folium artemisiae argyi, 45 parts of rhizoma acori graminei, 25 parts of mint and 25 parts of clove.

The preparation method is the same as in example 1.

Example 5

70 parts of rhizoma atractylodis, 30 parts of magnolia officinalis, 30 parts of fructus forsythiae, 30 parts of honeysuckle, 50 parts of radix scutellariae, 70 parts of folium artemisiae argyi, 50 parts of rhizoma acori graminei, 30 parts of mint and 30 parts of clove.

The preparation method is the same as in example 1.

The Chinese medicinal spray with different formulations has effect of killing microorganisms

Nutrient agar medium: 15g of agar; 10g of peptone; 5g of beef extract; 5g of sodium chloride; distilled water 1000mL. Dissolving the other components except agar in distilled water, regulating pH to 7.2-7.4, adding agar, heating for dissolving, and steam sterilizing at 121deg.C for 20min.

Sha Baoshi agar medium: 10g of peptone; glucose 40g; 15g of agar; distilled water 1000mL. Mixing the above materials, heating to dissolve completely, adjusting pH to 5.6+ -0.2, and steam sterilizing at 115deg.C for 30 min.

Typical colonies of second-generation strains of staphylococcus aureus and escherichia coli are inoculated on a nutrient agar medium, typical colonies of second-generation strains of candida albicans are inoculated on Sha Baoshi agar medium, and the culture is carried out for 24 hours at the constant temperature of 37 ℃. After the completion of the culture, 4.0mL of PBS was added to the above-mentioned slant tube, and the mixture was repeatedly sucked and washed to remove the lawn. The wash solution was transferred to another sterile tube with a 5.0mL pipette, the bacteria were mixed well by shaking repeatedly about 80 times on the palm, and then diluted to a bacterial suspension with a bacterial count of about 10 ⁸CFU·mL^-1 in 1% (m/V) peptone-containing PBS for use.

Respectively sucking 0.l mL of the prepared staphylococcus aureus, escherichia coli and candida albicans bacterial suspension, respectively placing the bacterial suspensions into sterile test tubes filled with 0.5mL of the traditional Chinese medicine spray of examples 1-5, respectively sucking 0.5mL of the mixed liquid of the traditional Chinese medicine bacteria after 1,3, 5 and 10min of action, transferring the mixed liquid into another sterile test tube, adding 4.5mL of a neutralizing reagent (PBS (0.2% lecithin+1.0% glycine+0.5% sodium thiosulfate+2.0% tween 80), uniformly mixing, sucking 0.1mL of the sample liquid after 10min of action, inoculating the sample liquid into a plate culture medium (staphylococcus aureus, nutrient agar culture medium for escherichia coli and Sha Baoshi agar culture medium for candida albicans), and measuring the number of living bacteria by adopting a plate counting method.

84 Disinfectant and PBS (0.1 mL each) were inoculated into the plate medium as negative control and positive control, respectively. Blank plate medium for the same batch was used as a blank control.

Culturing in 37 deg.C incubator, culturing Staphylococcus aureus and Escherichia coli for 48 hr, culturing Candida albicans for 72 hr, recording colony count, and calculating sterilization rate. The results are shown in Table 1.

Table 1 Chinese medicinal spray disinfectant has effect of killing microorganisms

The results in Table 1 show that the disinfectant prepared by the formula of example 3 has the best sterilization effect and has a sterilization effect of 100% on staphylococcus aureus, escherichia coli and candida albicans after 5 minutes of action. Although the sterilizing effect of the disinfectants prepared in the examples 1,2, 4 and 5 is lower than that of the disinfectant prepared in the example 3, the sterilizing effect is obvious, which indicates that the compatibility of the traditional Chinese medicines used in the invention has better sterilizing effect.

Sterilizing effect of Chinese medicine spray on air

A closed room with the volume of 20-25 m ³ is selected, and the traditional Chinese medicine spray of the example 3 is adopted to carry out spray disinfection according to the usage amount of 5mL/m ³ for 30min. Sampling by adopting a six-level sieve mesh impact type air microorganism sampler (QY-6 type of Beijing medical science and technology Co., ltd.) and setting two sampling points, wherein the sampling height is 1m, the distance is 1m from a wall, sampling is respectively carried out for 10min before disinfection and after disinfection, the sampled plates are placed at 37 ℃ for 48 hours, the colony number of each plate is recorded, the test is repeated for 3 times, and the extinction rate is calculated. The results are shown in Table 2.

Rate of extinction = (number of colonies before sterilization-number of colonies after sterilization)/number of colonies before sterilization × 100%

Table 2 air disinfection effect of Chinese medicinal spray (n=3, x±s)

The result shows that the Chinese medicinal spray has the extinction rate of more than 90% on natural bacteria in the air, and meets the disinfection requirement of the disinfection technical specification that the extinction rate of the natural bacteria in the air is more than 90%.

Determination of air disinfection maintaining time effect of traditional Chinese medicine spray

A closed room with the volume of 20-25 m ³ is selected, and the traditional Chinese medicine spray of the example 3 is adopted to carry out spray disinfection according to the usage amount of 5mL/m ³ for 30min. Sampling by adopting a six-level sieve mesh impact type air microorganism sampler (QY-6 type of Beijing medical science and technology Co., ltd.) and setting two sampling points, wherein the sampling height is 1m, the distance is 1m from a wall, the sampling is respectively carried out for 10min before disinfection, after disinfection, 1h after disinfection, 2h after disinfection, 4h after disinfection and 8h after disinfection, the sampled plates are placed at 37 ℃ for culturing for 48 hours, the colony number of each plate is recorded, and the extinction rate is calculated. The results are shown in Table 3.

TABLE 3 air disinfection maintenance time effect of traditional Chinese medicine spray disinfectant

The result shows that the extinction rate of natural bacteria in the air is more than 90% in 4 hours after disinfection, the extinction rate of natural bacteria in the air is 88.57% in 8 hours after disinfection, the disinfection effect is good, and the disinfection effect time is long.

Acute oral toxicity test of Chinese medicinal spray

20 Kunming mice were taken, each half of the male and female mice, and no water was forbidden for 12 hours. The traditional Chinese medicine spray of example 3 was used to irrigate the stomach at a dose of 5000mg/kg body weight (i.e. 1mL per 10g body weight). The blank control group was given an equal volume of physiological saline.

The test animals were continuously observed for 14d, and the daily death, weight change, food intake and other changes were recorded, and the results are shown in Table 4.

TABLE 4 acute toxicity test results

Note that: and v represents that the state occurs and x represents that the state does not occur.

The test results show that no 1 case of death occurs at the given dose, and no obvious poisoning phenomenon occurs. The toxicity of the medicine is smaller, the half lethal dose (LD ₅₀) is more than 5000mg/kg body weight, and the medicine is practically nontoxic according to the toxicity evaluation of the disinfectant.

Skin and eye irritation test of Chinese medicinal spray

The hairs of about 3X 3cm ² on both sides of the spine of the back of the rabbit were cut off 24 hours before the test, without damaging the epidermis. The next day, 0.5mL of the traditional Chinese medicine spray disinfectant of example 3 is dripped on 2-4 layers of gauze with the same size and applied on one side of the gauze to remove skin, then covered by a layer of non-irritating plastic film or oilpaper, and then fixed by a non-irritating adhesive tape. The other side was peeled off the skin as a blank (or solvent control). The application time is 4 hours. After the test is completed, the residual test substance is removed with warm water or a non-irritating solvent. Local skin reactions were observed 1h, 24h and 48h after removal of the test subjects, respectively.

After the skin of the rabbit is tested, the results show that the skin has no edema and erythema, and the skin is classified as non-irritating according to the skin irritation intensity.

Sucking 1 drop of the traditional Chinese medicine spray disinfectant of the embodiment 3, dripping into conjunctival sac of one side eye of a rabbit, dripping physiological saline into the other side eye of the rabbit as a contrast, passively closing the eyes for 4s, and flushing with the physiological saline after 30 s. The damage and recovery of conjunctiva, iris and cornea of rabbit eye were visually observed 1h,24h, 48h, 72h,7d, 14d, 21d after eye drop.

The results of eye irritation showed that conjunctiva, iris, cornea were all clear of turbidity, congestion, edema and secretion appearance, classified as non-irritating by eye irritation intensity.

Corrosion test of Chinese medicinal spray

Soaking stainless steel, aluminum sheet and carbon steel in a detergent with surface activity for 10min, removing oil completely, and cleaning; the oxide layers on the two sides and the peripheral surface of the metal sheet are ground by 120-size water sand paper, and then are washed by tap water. The diameter, thickness, pore size (to the nearest 0.1 mm) of the sheet were measured. Degreasing again with absolute ethanol. Drying in a 50 ℃ incubator for 1h, and weighing after the temperature of the mixture is reduced to room temperature. Then respectively soaking the materials in 200mL of the traditional Chinese medicine spray of the example 3, taking out after the materials are acted for 72 hours, flushing the materials with tap water, removing corrosion products with a brush or other soft appliances, absorbing water with coarse filter paper, placing the materials in a flat dish filled with filter paper, placing the flat dish in a 50 ℃ incubator, drying the flat dish for 1 hour, taking the flat dish with the filter paper by forceps, and weighing the flat dish on a balance after the temperature of the flat dish is reduced to room temperature. The weight change before and after soaking was measured, and the corrosion of the metal by the traditional Chinese medicine spray was evaluated, and the results are shown in table 5.

TABLE 5 Corrosion test results

Note that: r=8.7x107× (weight before soaking-weight after soaking)/s×t×d, s is the total area of the metal sheet (cm ²), t is the experimental time, and d is the metal material density (kg/m ³).

As can be seen from Table 5, the corrosion rate of the Chinese medicinal spray on the three metals is less than 0.01, and the Chinese medicinal spray is basically non-corrosive according to the corrosiveness grading standard.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the invention in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to the equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present invention still fall within the protection scope of the technical solution of the present invention.

Claims

1. The traditional Chinese medicine composition is characterized by comprising the following components in parts by weight:

2. The use of the Chinese medicinal composition of claim 1 in the preparation of an animal breeding environment disinfectant.

3. The traditional Chinese medicine spray for disinfecting animal cultivation environment is characterized by being prepared from the following components in parts by weight:

4. The traditional Chinese medicine spray for disinfecting animal raising environments according to claim 3, which is characterized by being prepared from the following components in parts by weight:

5. The method for preparing the traditional Chinese medicine spray for disinfecting animal raising environments according to claim 3 or 4, which is characterized by comprising the following steps:

6. The method for preparing the traditional Chinese medicine spray for disinfecting animal cultivation environment according to claim 5, wherein,

The conditions for supercritical CO ₂ fluid extraction in the step (1) are as follows: the extraction pressure is 25MPa, the extraction temperature is 55 ℃, the resolution I pressure is 7.5MPa, the resolution I temperature is 34 ℃, and the extraction time is 2h.

7. The method for preparing the traditional Chinese medicine spray for disinfecting animal cultivation environment according to claim 5, wherein,

The fructus forsythiae extraction method in the step (2) comprises the following steps: decocting with 8 times of water for 3 times each for 1.5 hours, filtering, mixing filtrates, concentrating the filtrate under reduced pressure below 60deg.C to obtain fluid extract with relative density of 1.10-1.20, cooling, adding 4 times of ethanol, stirring, standing for 2 hours, filtering, recovering ethanol from the filtrate under reduced pressure, concentrating the concentrate to 1L to obtain concentrate B;

8. The method for preparing the traditional Chinese medicine spray for disinfecting animal cultivation environment according to claim 5, wherein,

The extraction method of the volatile oil of the mugwort leaf, the grassleaf sweelflag rhizome, the peppermint and the clove in the step (3) comprises the following steps: soaking folium Artemisiae Argyi, rhizoma Acori Graminei, herba Menthae, and flos Caryophylli in water for 2-3 hr, extracting in volatile oil extractor for 6 hr, standing, collecting volatile oil, and drying with anhydrous sodium sulfate.

9. Use of a traditional Chinese medicine spray prepared by the method of any one of claims 5 to 8 in disinfection of animal raising environments.

10. A method for disinfecting an animal breeding environment, which is characterized in that the traditional Chinese medicine spray prepared by the method according to any one of claims 5-8 is used for spraying and disinfecting the animal breeding environment according to the usage amount of 5mL/m ³.