CN117503649A - Lotus leaf extract and softening hair care composition containing same - Google Patents
Lotus leaf extract and softening hair care composition containing same Download PDFInfo
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- CN117503649A CN117503649A CN202311477402.6A CN202311477402A CN117503649A CN 117503649 A CN117503649 A CN 117503649A CN 202311477402 A CN202311477402 A CN 202311477402A CN 117503649 A CN117503649 A CN 117503649A
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/002—Preparations for repairing the hair, e.g. hair cure
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to the technical field of daily cosmetics, and particularly relates to a lotus leaf extract and a softening and hair care composition containing the same. The lotus leaf extract contains abundant lotus leaf fermented amino acid molecules, can moisten hair core, supplement nutrition ingredients for hair, strengthen hair protein integrity, repair damaged hair scales, and reduce hair tip bifurcation caused by head heating injury and mechanical injury. Especially, the hair conditioner is applied to a stay-type hair care product containing sodium polyaspartate, and can synergistically enhance the effect of the product on smoothing and repairing hair scales, so that the hair care and repairing effect of the product is improved, hair is smooth and soft, the winding degree of hair is reduced, the pulling of the hair is reduced, the hair conditioner is soft and fresh, the damaged state of the hair is improved, and the glossiness of the hair is effectively improved.
Description
Technical Field
The invention belongs to the technical field of daily cosmetics, and particularly relates to a lotus leaf extract and a softening and hair care composition containing the same.
Background
At present, natural source materials commonly used in the market for claiming hair care efficacy are ginseng, argan oil, hydrolyzed keratin, ginger extract, rice washing water and the like. Lotus is a plant of the genus Nelumbo of the family Nymphaeaceae, and can be used as medicine, such as rhizoma Nelumbinis, folium Nelumbinis, petiole, stamen Nelumbinis, plumula Nelumbinis, and plumula Nelumbinis. The lotus leaves in China are rich in resources and wide in distribution, modern pharmacological researches show that the lotus leaves have the functions of resisting oxidation, resisting aging, clearing heat, reducing blood fat, reducing cholesterol, treating cardiovascular and cerebrovascular diseases and the like, and as the lotus leaves are deeply researched, the lotus leaves are gradually applied to the field of cosmetics due to the fact that the lotus leaves contain alkaloids and flavonoid compounds and the lotus She Zijie effect, and are manufactured into various skin care cream.
Researches show that the lotus leaves have strong antioxidation, detumescence and bacteriostasis effects, and are mostly used as an antioxidation active ingredient and a bacteriostasis ingredient in the field of cosmetics. For example, patent number CN113476360a, "a composition for improving eye bags, a preparation method and an application thereof", discloses a skin care composition with functions of inhibiting collagen degradation, resisting oxidation, digesting fat and the like, which takes dihydroartemisinin and lotus leaf extract as main active ingredients for improving eye bags. Also, for example, patent number CN103415299a, composition for antioxidation and reducing harmful effects of asian dust on skin, discloses a composition for antioxidation and reducing harmful effects of dust on skin using chinaberry bark extract and lotus leaf extract as a novel skin irritation-reducing agent.
For the extraction of the active components of lotus leaves, a traditional heating and steaming process is mostly adopted, for example, patent No. CN113786367A, a preparation method and application of lotus leaf extract, discloses a method for extracting flavonoid substances of lotus leaves by a heating acid hydrolysis method. But has simple extraction process, complex components and certain pungent components. Some of the lotus leaf cell secondary metabolites are extracted by adopting an enzymolysis technology, for example, patent number CN104706554A (preparation method and application of lotus leaf cell secondary metabolite freeze-dried powder) discloses a method for preparing lotus leaf cell secondary metabolite freeze-dried powder with anti-aging function by using cellulase and pectase to perform combined digestion; the method is novel, but the products are cell secondary metabolites and flavonoid products, and the method is suitable for the field of anti-aging or antioxidation of skin care products, and has almost zero hair care effect on hair.
Therefore, the lotus leaf extract is mainly used for resisting oxidation, aging and bacteria in skin care products in the cosmetic field at present, and is rarely used for excavating hair products. The inventor finds that the components in the lotus leaf contain cellulose, protein and a large amount of amino acids such as aspartic acid, serine, leucine and the like besides the commonly reported flavonoid substances and alkaloids, are rich in variety and have good development and utilization prospects.
Disclosure of Invention
The primary aim of the invention is to provide an extraction method of lotus leaf extract, the lotus leaf extract obtained by fermenting lotus leaf raw materials by a special method contains a large amount of amino acid and polysaccharide molecules, and the lotus leaf extract can be applied to hair products to provide nutrition for hair, repair and rebuild hair damage, repair hair tip bifurcation and improve hair health, and especially can be applied to stay-type hair care products containing sodium polyaspartate to synergistically improve the effect of smoothing and repairing hair scales of the products, so that the hair care and repairing effects of the products are improved.
The invention realizes the aim through the following technical scheme:
the invention provides a preparation method of the lotus leaf extract, which comprises the following steps:
(1) Cutting lotus leaf material, soaking in water, regulating temperature to 45-55deg.C, and regulating pH to 4.5-5.5;
(2) Adding 0.1-1% of cellulase and xylanase mixture for crude extraction, and filtering to obtain crude extract;
(3) Regulating the temperature of the crude extract to 30-39deg.C, adding 0.5-1.0% Aspergillus niger, adding 1-2% corn starch, fermenting for 24-72 hr, heating to 60-70deg.C, and sterilizing for 30-40 min to obtain crude fermentation broth;
(4) Filtering and separating the crude fermentation liquor, concentrating the filtrate to obtain a refined extract, and freeze-drying to obtain the lotus leaf extract.
Further, in the step (1), the pH regulator used for regulating the pH comprises one or more of citric acid, hydrochloric acid, lactic acid, sodium citrate, sodium hydroxide or potassium hydroxide; preferably citric acid.
Further, in the step (2), the ratio of the cellulase to the xylanase is 4-8:1.
in the step (2), preferably, 0.4-0.6% of the mixture of cellulase and xylanase is added for crude extraction, and lotus fibers and cell walls in lotus leaves are decomposed in the crude extraction process, so that amino acid and protein components of lotus leaf cells are released.
Further, in the step (2), the crude extraction is carried out after stirring for 18-24 hours at the rotating speed of 10-40r/min, so that the mixture of cellulase and xylanase can fully hydrolyze the lotus leaf raw material.
Further, in the step (2), filtration was performed using 200 mesh filter cloth.
In the step (3), the protein of lotus leaf is decomposed by protease generated in the growth process of aspergillus niger, so that small molecules of amino acids such as aspartic acid, serine and lysine in lotus leaf are obtained to a large extent.
In the step (4), the ultra-filtration membrane of the ultrcraft 10 is used for filtering and separating, macromolecular substances and thalli in the crude fermentation broth are further separated, and the filtrate is concentrated to obtain the lotus leaf extract with high content of micromolecular amino acid.
The invention also provides the lotus leaf extract prepared by the method, and the total amino acid content in the lotus leaf extract is more than 80%.
The invention also provides application of the lotus leaf extract in hair care products, and the lotus leaf extract contains abundant lotus leaf fermentation amino acid molecules, can moisten hair cores, supplements nutrition components for hair, enhances the protein integrity of the hair, repairs damaged hair scales, and reduces hair tip bifurcation caused by head heating damage and mechanical damage. Especially, the product can be applied to the stay-type hair care product containing sodium polyaspartate, and the effects of smoothing and repairing hair scales of the synergistic product can be synergistically improved, so that the hair care and repairing effects of the product are improved.
Another object of the present invention is to provide a hair care composition containing the lotus leaf extract, which has various uses, can be used in both dry and wet hair conditions, smooth and soft hair, reduces the degree of hair entanglement, reduces hair pulling, and has the effects of softening and refreshing the hair after rinsing, improving the damaged hair state, improving the glossiness of the hair, and has no residue on the hands, is fresh and not sticky after drying, is quick-dried and not sticky after being applied on the hands, and does not need to wash hands.
The invention provides a hair care composition containing the lotus leaf extract, which comprises the following components in percentage by weight: the lotus leaf extract is 0.01-10%, emulsifying agent is 0.1-3%, thickening stabilizer is 0.03-0.4%, softening care agent is 3-30%, hair conditioner is 0.001-10%, and pure water is added to 100%.
Furthermore, the hair conditioner comprises sodium polyaspartate, the lotus leaf extract can be synergistic with the sodium polyaspartate in the composition, the restoration effect of the composition is improved, hair is healthy, smooth and soft, the hair is easy to treat, and the refreshing feel of the product is not affected by the addition of the lotus leaf extract.
Further, in addition to the above components, at least one of the following components may be contained: chelating agent, pigment, essence, non-volatile conditioner, pH regulator, appearance modifier, ultraviolet absorbent, antiseptic or other plant extractive solution, etc.
The invention has the beneficial effects that:
the preparation method of the lotus leaf extract is simple and environment-friendly, the prepared lotus leaf extract is rich in amino acid small molecules, has an excellent repairing effect on hair, and the amino acid molecules can infiltrate hair, provide nutrition for the hair, repair damaged hair, enable the hair to be smooth and soft, be easy to manage, restore a health state, and can also realize synergistic effect of sodium polyaspartate, so that the hair care and softening functions of the composition are improved.
The hair care composition disclosed by the invention has multiple purposes through scientific collocation of raw materials, can be used as a hair conditioner or a hair mask after the shampoo is used, has a good effect of wetting hair, softening and untangling hair, smoothening hair scales, rapidly forming a protective film, closing the effect of forked hair, reducing heat damage and mechanical damage of the hair, and smoothing and brightening hair after washing and drying.
The hair cream can also be used in a semi-dry or full-dry state of hair, can be free of flushing, is convenient to use, and has the advantages of smoothness, softness and brightness of hair after being used, repairing damaged hair, freshness, no burden, low residue after being coated, no need of flushing hands, low skin irritation, good stability, easiness in coating and spreading, and more smoothness, softness and brightness of hair, practicability and convenience.
Drawings
Fig. 1: and rinsing the hair to obtain the hair scale state on the surface of the hair.
Fig. 2: example 5 treated hair surface hair scale status.
Fig. 3: comparative example 1 hair surface hair scale status after treatment.
Fig. 4: comparative example 5 hair surface hair scale status after treatment.
Detailed Description
The present invention will be described in further detail with reference to test examples and specific embodiments, but it should not be construed that the scope of the subject matter of the present invention is limited to the following examples, and all techniques realized based on the present invention are within the scope of the present invention.
Example 1:
(1) Cutting lotus leaf raw materials, soaking in water, adjusting the temperature to 45 ℃, and adjusting the pH to 4.5 by using citric acid;
(2) Adding 0.4% of cellulase and xylanase mixture (the ratio of cellulase to xylanase is 4:1), stirring at 30r/min for 24 hr, performing crude extraction, and filtering with 200 mesh filter cloth to obtain crude extract;
(3) Regulating the temperature of the crude extract to 30 ℃, adding 0.5% of Aspergillus niger, adding 1% of corn starch, fermenting for 24 hours, heating to 60 ℃ and sterilizing for 30 minutes to obtain crude fermentation liquor;
(4) The crude fermentation broth was isolated by filtration using an Ultrcraft 10 ultrafiltration membrane, and the filtrate was concentrated to give a fine extract, which was freeze-dried to give the lotus leaf extract described in example 1.
Example 2:
(1) Cutting lotus leaf raw materials, soaking in water, adjusting the temperature to 50 ℃, and adjusting the pH to 5.0 by using citric acid;
(2) Adding 0.5% of cellulase and xylanase mixture (the ratio of cellulase to xylanase is 6:1), stirring at 30r/min for 24 hr, performing crude extraction, and filtering with 200 mesh filter cloth to obtain crude extract;
(3) Regulating the temperature of the crude extract to 35 ℃, adding 0.5% of Aspergillus niger, adding 1% of corn starch, fermenting for 72 hours, heating to 60 ℃ and sterilizing for 30 minutes to obtain crude fermentation liquor;
(4) The crude fermentation broth was isolated by filtration using an Ultrcraft 10 ultrafiltration membrane, and the filtrate was concentrated to give a fine extract, which was freeze-dried to give the lotus leaf extract described in example 2.
Example 3:
(1) Cutting lotus leaf raw materials, soaking in water, adjusting the temperature to 55 ℃, and adjusting the pH to 5.5 by using citric acid;
(2) Adding 0.6% of cellulase and xylanase mixture (the ratio of cellulase to xylanase is 8:1), stirring at 30r/min for 24 hr, performing crude extraction, and filtering with 200 mesh filter cloth to obtain crude extract;
(3) Regulating the temperature of the crude extract to 30 ℃, adding 1.0% of Aspergillus niger, adding 2% of corn starch, fermenting for 24 hours, heating to 70 ℃ and sterilizing for 30 minutes to obtain crude fermentation liquor;
(4) The crude fermentation broth was isolated by filtration using an Ultrcraft 10 ultrafiltration membrane, and the filtrate was concentrated to give a fine extract, which was freeze-dried to give the lotus leaf extract described in example 3.
The extracts of examples 1-3 above were analyzed for small molecule content and the results are shown in Table 1 below:
TABLE 1
The lotus leaf extract described in example 1 above was used in hair care compositions, and the formulation is shown in table 2 below, prepared by mixing and stirring as is conventional in the art:
TABLE 2
The stability test, chick embryo irritation test, hair tress comb property, gloss, skin feel evaluation for trial use by people and hair scale repair effect test were performed on the above examples 4 to 6 and comparative examples 1 to 3 respectively, and the results are shown as follows:
1. stability test
Examples 4-6 and application comparative examples 1-3 were each placed under different temperature conditions for 30 days, and the stability results are shown in Table 3 below:
TABLE 3 Table 3
| Conditions (conditions) | Example 4 | Example 5 | Example 6 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| -15℃ | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change |
| 5℃ | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change |
| 25℃ | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change |
| 40℃ | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change |
| 48℃ | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change | No obvious change |
As can be seen from the results in Table 3, the compatibility of the lotus leaf extract with the system is good, and the stability of the system is not affected.
2. Chick embryo irritation test
Chick embryo irritation test was performed for examples 4-6 and comparative examples 1-4, comparative example 4 is a conventional leave-on hair conditioner paste system, evaluation was performed according to the chick embryo vascular response acute irritation condition at the 8 th to 10 th day of incubation, score grades are shown in Table 4, and the results evaluation table of the irritation scoring method is shown in Table 5.
TABLE 4 Table 4
TABLE 5
| Stimulation scoring | Irritation classification |
| IS＜1 | No irritation |
| 1≤IS＜5 | Light irritation |
| 5≤IS＜10 | Moderate irritation |
| IS≥10 | Strong irritation/corrosiveness |
The chicken embryo stimulation scoring method comprises the following steps: the stimulation score (IS) was calculated using the reaction time method, using the following formula, with the result remaining two decimal places:
IS＝(301－secH)×5÷300+(301－secL)×7÷300+(301－secC)×9÷300
wherein:
secH (bleeding time): the average time in seconds(s) for onset of bleeding was observed on the CAM film;
secL (vascular thawing time): the average time in seconds(s) for onset of vascular thawing is observed on the CAM membrane;
secC (clotting time): the average time in(s) for the onset of clotting was observed on the CAM film.
The test subjects were classified for ocular irritation according to the calculated IS values as shown in table 5, and the test results are shown in table 6 below:
TABLE 6
As can be seen from the test results of examples 4-6 and comparative examples 1-3, the lotus leaf extract of the present invention is mild and has low irritation, and does not affect the mild safety of the system, and as can be seen from the results of examples 4-6 and comparative example 4, the composition of the present invention has a milder than the leave-on hair care products made of conventional cationic surfactants, and is more friendly to the skin.
3. Comb hair tresses, gloss and skin feel evaluation for crowd trial
Examples 4-6 and comparative examples 1-5 were each tested for dry and wet combing work, gloss, and after any selection of 30 volunteers for testing, the freshness assessment test, and comparative example 5 was a better, well-known leave-in hair conditioner on the market.
The test method of dry and wet comb performance is as follows:
(1) Selecting the same batch of hair pieces, cleaning and airing, soaking the hair pieces in 5% K12 solution, washing the hair pieces cleanly with clear water after constant-temperature water bath at 40 ℃ for 30 minutes, and hanging the hair pieces in a constant-temperature constant-humidity room at 21+/-1 ℃ and RH 50+/-10% for more than 1 hour until airing. Then placing the mixture into a constant temperature and humidity box for more than 24 hours; the hair pieces are numbered. Starting an SK-7B dynamic carding instrument, testing a basic value of dry hair, and selecting hair bundles with the basic value of 0.4J-0.7J for the next step, so as to ensure that 5 hair bundles exist in each group of sample test hair bundles;
(2) 2ml of the hair care composition is taken to evenly coat the hair bundle, the two hands gently feel the hair bundle for 30 rounds from top to bottom, the hair bundle is washed cleanly by the same water temperature and water flow, the redundant water is removed by stroking, and the washing is repeated for one time. Testing hair bundles in the same environment by using an SK-7B dynamic carding instrument, and respectively testing the basic values of static and dynamic wet hair, the wet hair test value after sample application and the dry hair test value after sample application; the standard deviation of the change rate of the dry hair combing work, the static wet hair combing work and the dynamic wet hair combing work is ensured to be within 10 percent.
The gloss test method is as follows:
(1) Selecting hair pieces in the same batch, cleaning and airing, and selecting hair bundles with weight of 5.8+/-0.2 g for the next step; the hair piece is fixed by a clamp, and is combed smoothly by a comb, and after the surface of the hair piece is leveled, the hair piece is placed on the surface of the leveled hair piece by a precise gloss meter for measurement. Five test points are respectively taken from the front side and the back side. Recording the basic glossiness value of the surface of the hair piece before sample receiving, screening 5 hair bundles with similar basic values, and testing;
(2) Taking 2ml of hair care composition, uniformly coating the hair bundle, then flushing the hair bundle with water flow with the flow rate of 4litres/minute for 1min at 37 ℃, and hanging the hair bundle in a constant temperature and humidity room with the RH of 50+/-10% at 21+/-1 ℃ for more than 1h until the hair bundle is dried. Then placing the mixture into a constant temperature and humidity box for more than 24 hours; the gloss of the test specimens was measured as the test value after sampling. The lower the change rate of the carding work is reduced in the dry and wet carding test, the smaller the friction force is, the more flexible is; the larger the glossiness test change rate is, the better; the score of the freshness after the crowd is tried is 1-10, and the comparative example 6 is used as a judgment standard of 5, the score is more than 5 when the freshness is more excellent than the score, the score is less than 5 when the freshness is greasy than the score, the higher the score is, the more refreshing is represented, and the test result is shown in the following table 7.
TABLE 7
According to the test results of the above examples 4-6 and comparative example 1, as the lotus leaf extract increases, the dry hair comb property effect of the sample increases, and the glossiness increases, indicating that the addition of the lotus leaf extract is beneficial to improving the dry hair comb property effect of the product and improving the glossiness of hair; from comparison of example 4 and comparative examples 1-3, the addition of lotus leaf extract and sodium polyaspartate as hair conditioner can cooperate with each other to synergistically improve the dry hair combing effect and glossiness of the formulation; from the test results of examples 4 to 6 and comparative examples 4 and 5, the dry and wet combability and glossiness of the hair conditioner are superior to those of comparative examples (conventional cationic active and leave-on hair conditioner composed of fatty alcohol), which shows that the hair conditioner has more excellent hair softening effect and hair glossiness improving effect. As is clear from the comparison of examples 4-6 with comparative examples 4 and 5, the present invention provides a better post-use freshness, a fresh and non-sticky hair, and a fresh palm without the need to clean the hands.
4. Hair scale repairing effect
The test method is as follows:
(1) Hair bundle damage treatment: spreading the cleaned hair piece in a stainless steel square plate, adding a mixed aqueous solution of 1% hydrogen peroxide and 2% rehydration, keeping the temperature in a HWS26 electric heating constant-temperature water bath kettle at 40 ℃ for 2 hours (turning over after 1 hour), taking out the hair piece, washing the hair piece cleanly with clear water, neutralizing for 5 minutes with 10g/L anhydrous citraconic acid, lightly carding the mixed hair piece in order by using a comb, and hanging the hair piece in a constant-temperature constant-humidity room for more than 4 hours.
(2) The state of the hair scales on the front surface of the hair treatment was observed by SEM electron scanning electron microscopy, and the state of the hair scales after rinsing was recorded as shown in fig. 1.
(3) And (3) taking 0.2g of sample, coating the sample on the hair bundle, and observing the state of the hair scales on the surface of the hair after the hair is treated by adopting an SEM (scanning electron microscope), and recording.
Samples of example 5 and comparative examples 1 and 5 were applied to hair strands, respectively, and the hair strand electron microscope scan pictures of the three samples were shown in fig. 2, 3 and 4, respectively.
From the test results of example 5, comparative example 1 and comparative example 5, the composition of the present invention has a far higher effect of repairing the closure agent of the hair follicle scales than the commercially available conventional leave-on hair conditioner products; from comparison of the results of example 5 and comparative example 1, it is known that the addition of the lotus leaf extract of the present invention can further enhance the hair scale restoration effect of the product, make the hair surface smoother, restore damaged hair scales, and improve the hair quality state.
It will be understood that the above embodiments are further illustrative of the present invention and are not intended to limit the scope of the invention, and that all other modifications and variations which may be obtained without the inventive effort by those skilled in the art are within the scope of the invention.
Claims (10)
1. The preparation method of the lotus leaf extract is characterized by comprising the following steps of:
(1) Cutting lotus leaf material, soaking in water, regulating temperature to 45-55deg.C, and regulating pH to 4.5-5.5;
(2) Adding 0.1-1% of cellulase and xylanase mixture for crude extraction, and filtering to obtain crude extract;
(3) Regulating the temperature of the crude extract to 30-39deg.C, adding 0.5-1.0% Aspergillus niger, adding 1-2% corn starch, fermenting for 24-72 hr, heating to 60-70deg.C, and sterilizing for 30-40 min to obtain crude fermentation broth;
(4) Filtering and separating the crude fermentation liquor, concentrating the filtrate to obtain a refined extract, and freeze-drying to obtain the lotus leaf extract.
2. The method of claim 1, wherein in the step (1), the pH adjuster used for adjusting the pH comprises one or more of citric acid, hydrochloric acid, lactic acid, sodium citrate, sodium hydroxide, and potassium hydroxide.
3. The method of claim 1, wherein in step (2), the ratio of cellulase to xylanase is 4-8:1.
4. the method of claim 1, wherein in step (4), the separation by filtration is performed using an ultrcraf10 ultrafiltration membrane.
5. A lotus leaf extract prepared by the method of any one of claims 1-4.
6. The lotus leaf extract according to claim 5, wherein the total amino acid content in the lotus leaf extract is 80% or more.
7. Use of the lotus leaf extract of claim 5 or 6 in hair care products.
8. The use according to claim 7, in hair care products containing sodium polyaspartate.
9. A hair care composition comprising the following components in weight percent: the lotus leaf extract as claimed in claim 5, wherein the total content of the lotus leaf extract is 0.01-10%, the total content of the emulsifying agent is 0.1-3%, the total content of the thickening stabilizer is 0.03-0.4%, the total content of the softening care agent is 3-30%, the total content of the hair conditioning agent is 0.001-10%, and the total content of the pure water is 100%.
10. The hair care composition of claim 9, wherein the hair conditioning agent comprises sodium polyaspartate.
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