CN116925912A - Fluorescent quantitative PCR amplification thermal cycle module - Google Patents
Fluorescent quantitative PCR amplification thermal cycle module Download PDFInfo
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- CN116925912A CN116925912A CN202310877962.4A CN202310877962A CN116925912A CN 116925912 A CN116925912 A CN 116925912A CN 202310877962 A CN202310877962 A CN 202310877962A CN 116925912 A CN116925912 A CN 116925912A
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- 230000003321 amplification Effects 0.000 title claims abstract description 41
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 41
- 238000003753 real-time PCR Methods 0.000 title claims abstract description 23
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 32
- 229910052802 copper Inorganic materials 0.000 claims abstract description 32
- 239000010949 copper Substances 0.000 claims abstract description 32
- 238000005057 refrigeration Methods 0.000 claims abstract description 29
- 239000013078 crystal Substances 0.000 claims abstract description 18
- 238000009413 insulation Methods 0.000 claims abstract description 4
- 239000004696 Poly ether ether ketone Substances 0.000 claims description 10
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 claims description 10
- 229920002530 polyetherether ketone Polymers 0.000 claims description 10
- 238000005382 thermal cycling Methods 0.000 claims description 8
- 239000004033 plastic Substances 0.000 claims description 7
- 238000003752 polymerase chain reaction Methods 0.000 description 28
- 239000002390 adhesive tape Substances 0.000 description 10
- 238000003825 pressing Methods 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 230000008602 contraction Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000009529 body temperature measurement Methods 0.000 description 6
- 238000013461 design Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000004065 semiconductor Substances 0.000 description 6
- 239000000919 ceramic Substances 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 5
- 230000017525 heat dissipation Effects 0.000 description 5
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 4
- 229910052782 aluminium Inorganic materials 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- 230000002035 prolonged effect Effects 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 206010064571 Gene mutation Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a fluorescence quantitative PCR amplification thermal cycle module, which comprises: the refrigerating chip comprises a copper level, a crystal grain and a power line; the copper level is arranged on the surface of the refrigeration chip, and the crystal grains are welded on the copper level; the device also comprises a radiator and a reaction tank; the copper level of refrigeration chip sets up the radiator with the intermediate position of reaction tank, the copper pole through insulating heat conduction sticky tape direct fixation with form whole module on the reaction tank with the radiator, the reaction tank with the surface of radiator carries out insulation treatment, through directly fixing the copper pole of refrigeration chip on reaction tank and the radiator, whole module's heat conduction efficiency improves, promotes the thermal cycle speed, precision and the reliability of amplification module.
Description
Technical Field
The invention provides a fluorescence quantitative PCR amplification thermal cycle module, and belongs to the field of scientific instruments.
Background
A fluorescent PCR instrument (Fluorescent PCR Instrument) is a laboratory instrument for performing a fluorescent Polymerase Chain Reaction (PCR); PCR is a technique for amplifying DNA (or RNA) fragments, and a fluorescent PCR instrument is an instrument for monitoring a PCR reaction in real time by a fluorescent probe during the PCR process;
a fluorescent PCR instrument uses a special fluorescent probe to detect the amplification of a specific DNA sequence in a PCR reaction; these fluorescent probes typically include a primer for amplification and a fluorescent label that specifically binds to the target sequence. During the PCR reaction, the fluorescent probe is combined with target DNA of the amplified product, and the PCR reaction is monitored in real time through the change of the fluorescent signal;
the fluorescent PCR instrument has the characteristics of high sensitivity, real-time monitoring, quantitative analysis and the like, so that scientific researchers can accurately and rapidly detect and analyze the amplification condition of a target DNA sequence; fluorescent PCR is widely applied to the fields of gene expression analysis, pathogen detection, gene mutation identification and the like;
the thermal cycle amplification thermal cycle module is a core functional module of the fluorescent PCR instrument, and the reliability, the consistency and the temperature rise and fall rate of the thermal cycle amplification thermal cycle module are used for determining the quality and the service life of the fluorescent PCR instrument and play a decisive role in the product performance of the instrument. The fluorescent PCR instrument is applied to the fields of medical treatment and analysis, and has wide application in clinical diagnosis, gene detection, food safety, scientific research and the like; according to the technical scheme, technical innovation is carried out on a temperature control module of the fluorescent quantitative PCR instrument, and the quality and the service life of the PCR instrument are improved by adopting new materials, structures and testing methods; there are 8-hole, 16-hole, 32-hole, 48-hole, 96-hole and other product series on the market;
the existing fluorescent quantitative PCR amplification module adopts a refrigerating sheet which is provided with a ceramic or aluminum substrate, and the refrigerating or heating power generated by the crystal grains of the semiconductor refrigerating sheet after electric power is loaded can be transmitted to a reaction tank and a radiator through the ceramic or the substrate, so that the problems and the defects of high thermal resistance, low heat conduction efficiency, poor temperature consistency and the like exist; in addition, some PCR refrigerating sheets are directly welded on ceramic or aluminum substrates by adopting semiconductor crystal grains, and the reliability of the heat conduction amplification module in the welding mode is reduced because of expansion caused by heat and contraction caused by cold in the PCR thermal cycle process.
Disclosure of Invention
The invention provides a fluorescent quantitative PCR amplification thermal cycle module, which is used for solving the problem of low reliability of a heat conduction amplification module in the prior art, and adopts the following technical scheme:
a fluorescent quantitative PCR amplification thermal cycling module comprising: the refrigerating chip comprises a copper level, a crystal grain and a power line; the copper level is arranged on the surface of the refrigeration chip, and the crystal grains are welded on the copper level; the device also comprises a radiator and a reaction tank; the copper level of refrigeration chip sets up the radiator with the intermediate position of reaction tank, the copper pole through insulating heat conduction sticky tape direct fixation with form whole module on the reaction tank with the radiator, the reaction tank with the surface of radiator carries out insulation treatment, through directly fixing the copper pole of refrigeration chip on reaction tank and the radiator, whole module's heat conduction efficiency improves, promotes the thermal cycle speed, precision and the reliability of amplification module.
Preferably, the reaction tank is further fixed by adopting a plastic PEEK screw, the expansion curve of the fluorescent PCR is kept consistent for a long time by adopting the PEEK screw, and the expansion stress and the contraction stress can be enhanced by adopting the plastic PEEK screw to fix the reaction tank, so that the expansion curve of the fluorescent PCR is kept consistent in long-term use.
Preferably, the refrigeration chip further comprises a hot surface and a cold surface, the hot surface is in contact with the reaction tank, heat is absorbed from the reaction tank in a heat conduction mode, after the heat is absorbed by the hot surface, the heat is transferred from the hot surface to the cold surface through heat conduction, the cold surface is in contact with the radiator so as to quickly emit the heat into the surrounding environment, and the heat is absorbed from the reaction tank and quickly transferred to the cold surface through the design of the hot surface and the cold surface, so that efficient heat dissipation is realized, and the stability and reliability of the system are maintained.
Preferably, the device further comprises a pressing block and a temperature sensor, wherein the temperature sensor is arranged on the upper side of the reaction tank, the pressing block is arranged on the upper side of the temperature sensor and used for fixing the temperature sensor and the reaction tank, and the temperature sensor is ensured to be in stable contact with the reaction tank through combination of the pressing block and the temperature sensor, so that the accuracy and the reliability of temperature measurement are improved.
Preferably, the radiator comprises a fan bracket, a fan and a fan mesh enclosure; the fan support sets up the outside of fan is used for fixing the fan, the fan screen panel sets up the downside of fan is used for the protection the fan, through the combination of fan support, fan and fan screen panel, realizes the effective forced air cooling to the radiator, protects the fan from the harm of external object, promotes radiating effect and system's stability.
Through the technical scheme, the fluorescent quantitative PCR amplification thermal cycle module. The module comprises a refrigeration chip, wherein copper poles are welded with crystal grains and are directly fixed on a reaction tank and a radiator through an insulating heat conduction adhesive tape to form an integral module, and the surfaces of the reaction tank and the radiator are subjected to insulating treatment. The design improves the heat conduction efficiency and the heat circulation speed, precision and reliability of the amplification module.
In a further optimization scheme, the reaction tank is fixed by adopting a plastic PEEK screw, so that the thermal expansion and cold contraction stress is enhanced, and the amplification curve of fluorescent PCR is ensured to be consistent in long-term use. In addition, the refrigeration chip further comprises a hot surface and a cold surface, heat is absorbed from the reaction tank in a heat conduction mode, then the cold surface is contacted with the radiator, the heat is rapidly emitted to the surrounding environment, high-efficiency heat dissipation is achieved, and the stability and reliability of the system are maintained.
The module also comprises a pressing block and a temperature sensor, wherein the pressing block is used for fixing the temperature sensor and the reaction tank, so that stable temperature measurement is ensured. Finally, the radiator is composed of a fan bracket, a fan and a fan net cover, so that the effective air cooling of the radiator is realized, the fan is protected from being damaged by external objects, and the radiating effect and the stability of the system are improved.
Through the technical scheme, the fluorescent quantitative PCR amplification thermal cycle module can efficiently and stably perform PCR amplification, provides reliable temperature control and consistent amplification curve, and is suitable for application fields such as gene analysis, biomedical research and the like.
The invention has the following beneficial effects:
1. the module directly fixes the copper electrode of the semiconductor crystal grain to the PCR reaction tank and the radiator through a special refrigeration chip structure to form an integral module. The design greatly reduces the thermal resistance in the heat conduction process, quickens the heat transfer speed, and improves the speed, the precision and the reliability of the heat circulation. Meanwhile, the stability of the module is ensured by the insulating heat-conducting adhesive tape and insulating treatment, the service life of the product is prolonged, the refrigeration chip is not a semiconductor refrigeration chip adopting a traditional ceramic chip or an aluminum substrate as an upper substrate and a lower substrate, the refrigeration chip is innovated, the copper electrode welded with crystal grains is directly contacted with a reaction tank and a radiator through heat-conducting materials, and the heat-conducting medium materials are reduced, so that the generated heat is rapidly transferred to a target component.
2. The refrigerating chip of the module comprises a hot surface and a cold surface, and heat is absorbed from the reaction tank and rapidly dissipated into the radiator through heat conduction, so that the temperature is effectively reduced, and the stability of the system is maintained. In addition, the combination of the pressing block and the temperature sensor ensures the stable contact between the temperature sensor and the reaction tank, and improves the accuracy and the reliability of temperature measurement.
3. The adhesive insulating heat-conducting adhesive tape is used for fixing the copper poles of crystal grains, fixing the position of a refrigerating sheet, and meanwhile, the adhesive tape has the effect of buffering the stress of thermal expansion and cold contraction, so that the reliability is enhanced, and the installation surfaces of the heater and the reaction tank are subjected to insulating treatment, so that the heat-conducting adhesive tape is stable in the high-low temperature use process, and the service life of a product is prolonged.
Drawings
FIG. 1, an exploded view of a fluorescent quantitative PCR amplification thermal cycling module;
FIG. 2 is a schematic diagram of the structure of a refrigeration chip of a fluorescent quantitative PCR amplification thermal cycle module;
FIG. 3 is a schematic diagram of the installation of a fluorescent quantitative PCR amplification thermal cycling module;
FIG. 4 is a schematic diagram of a heat sink for a fluorescent quantitative PCR amplification thermal cycling module;
FIG. 5 is a schematic diagram of the cold and hot sides of a fluorescent quantitative PCR amplification thermal cycling module.
1. Briquetting; 2. a temperature sensor; 3. a reaction tank; 4. a refrigeration chip; 5. a heat sink; 6. a fan bracket; 7. a fan; 8. a fan guard; 9. copper grade; 10. a crystal grain; 11. a power line; 12. a hot face; 13. and (5) cooling the surface.
Detailed Description
The preferred embodiments of the present invention will be described below with reference to the accompanying drawings, it being understood that the preferred embodiments described herein are for illustration and explanation of the present invention only, and are not intended to limit the present invention.
1. Example 1:
a fluorescent quantitative PCR amplification thermal cycling module comprising: a refrigeration chip 4 comprising a copper level 9, a die 10 and a power line 11; the copper level 9 is arranged on the surface of the refrigeration chip 4, and the crystal grains 10 are welded on the copper level 9; the device also comprises a radiator 5 and a reaction tank 3; copper level of refrigeration chip 4 sets up the radiator with the intermediate position of reaction tank 3, copper pole 9 is directly fixed to through insulating heat conduction sticky tape reaction tank 3 with form whole module on the radiator 5, reaction tank 3 with the surface of radiator 5 carries out insulation treatment, through directly fixing the copper pole of refrigeration chip on reaction tank and the radiator, whole module's heat conduction efficiency improves, promotes the thermal cycle speed, precision and the reliability of amplification module.
Specifically, the reaction tank is further fixed by adopting a plastic PEEK screw, the amplification curve of the fluorescent PCR is kept consistent for a long time by adopting the PEEK screw, and the expansion and contraction stress can be enhanced by using the plastic PEEK screw to fix the reaction tank, so that the amplification curve of the fluorescent PCR is ensured to be consistent in long-term use.
Specifically, the refrigeration chip further comprises a hot surface 12 and a cold surface 13, the hot surface 12 is in contact with the reaction tank 3, heat is absorbed from the reaction tank 3 in a heat conduction mode, after the hot surface 12 absorbs the heat, the heat is transferred from the hot surface to the cold surface 13 through heat conduction, the cold surface 13 is in contact with the radiator 5 so as to quickly emit the heat into the surrounding environment, and the heat is absorbed from the reaction tank and quickly transferred to the cold surface through the design of the hot surface and the cold surface, so that efficient heat dissipation is realized, and the stability and reliability of the system are maintained.
Specifically, still include briquetting 1 and temperature sensor 2, temperature sensor 2 sets up the upside of reaction tank 3, briquetting 1 sets up the upside of temperature sensor 2 is used for fixing temperature sensor 2 with reaction tank 3, through briquetting and temperature sensor's combination, ensures temperature sensor and reaction tank's stable contact, improves temperature measurement's accuracy and reliability.
Specifically, the radiator 5 includes a fan bracket 6, a fan 7 and a fan guard 8; the fan bracket 6 is arranged on the outer side of the fan 7 and used for fixing the fan 7, the fan screen 8 is arranged on the lower side of the fan 7 and used for protecting the fan 7, and the fan is protected from being damaged by external objects through the combination of the fan bracket, the fan and the fan screen, so that the heat dissipation effect and the stability of a system are improved;
through the technical scheme, the fluorescent quantitative PCR amplification thermal cycle module is implemented. The module comprises a refrigeration chip, wherein copper poles are welded with crystal grains and are directly fixed on a reaction tank and a radiator through an insulating heat conduction adhesive tape to form an integral module, and the surfaces of the reaction tank and the radiator are subjected to insulating treatment. The design improves the heat conduction efficiency and the heat circulation speed, precision and reliability of the amplification module.
In a further optimization scheme, the reaction tank is fixed by adopting a plastic PEEK screw, so that the thermal expansion and cold contraction stress is enhanced, and the amplification curve of fluorescent PCR is ensured to be consistent in long-term use. In addition, the refrigeration chip further comprises a hot surface and a cold surface, heat is absorbed from the reaction tank in a heat conduction mode, then the cold surface is contacted with the radiator, the heat is rapidly emitted to the surrounding environment, high-efficiency heat dissipation is achieved, and the stability and reliability of the system are maintained.
The module also comprises a pressing block and a temperature sensor, wherein the pressing block is used for fixing the temperature sensor and the reaction tank, so that stable temperature measurement is ensured. Finally, the radiator is composed of a fan bracket, a fan and a fan net cover, so that the effective air cooling of the radiator is realized, the fan is protected from being damaged by external objects, and the radiating effect and the stability of the system are improved.
Through implementation of the technical scheme, the fluorescent quantitative PCR amplification thermal cycle module can efficiently and stably perform PCR amplification, provides reliable temperature control and consistent amplification curve, and is suitable for application fields such as gene analysis, biomedical research and the like;
the module directly fixes the copper electrode of the semiconductor crystal grain to the PCR reaction tank and the radiator through a special refrigeration chip structure to form an integral module. The design greatly reduces the thermal resistance in the heat conduction process, quickens the heat transfer speed, and improves the speed, the precision and the reliability of the heat circulation. Meanwhile, the insulating heat-conducting adhesive tape and insulating treatment ensure the stability of the module, the service life of the product is prolonged, the refrigeration chip is not a semiconductor refrigeration chip adopting a traditional ceramic chip or an aluminum substrate as an upper substrate and a lower substrate, the refrigeration chip is innovated, a copper electrode welded with crystal grains is directly contacted with a reaction tank and a radiator through heat-conducting materials, and the heat-conducting medium materials are reduced, so that the generated heat is rapidly transferred to a target component;
the refrigerating chip of the module comprises a hot surface and a cold surface, and heat is absorbed from the reaction tank and rapidly dissipated into the radiator through heat conduction, so that the temperature is effectively reduced, and the stability of the system is maintained. In addition, the combination of the pressing block and the temperature sensor ensures the stable contact between the temperature sensor and the reaction tank, and improves the accuracy and the reliability of temperature measurement;
the adhesive insulating heat-conducting adhesive tape is used for fixing the copper poles of crystal grains, fixing the position of a refrigerating sheet, and meanwhile, the adhesive tape has the effect of buffering the stress of thermal expansion and cold contraction, so that the reliability is enhanced, and the installation surfaces of the heater and the reaction tank are subjected to insulating treatment, so that the heat-conducting adhesive tape is stable in the high-low temperature use process, and the service life of a product is prolonged.
Claims (5)
1. A fluorescent quantitative PCR amplification thermal cycling module, comprising:
the refrigerating chip (4) comprises a copper grade (9), a crystal grain (10) and a power line (11);
the copper level (9) is arranged on the surface of the refrigeration chip (4), and the crystal grains (10) are welded on the copper level (9);
the device also comprises a radiator (5) and a reaction tank (3); copper level of refrigeration chip (4) sets up the intermediate position of radiator with reaction tank (3), copper pole (9) are through insulating heat conduction sticky tape direct fixation reaction tank (3) with form whole module on radiator (5), reaction tank (3) with the surface of radiator (5) carries out insulation treatment.
2. The fluorescent quantitative PCR amplification thermal cycling module according to claim 1, wherein:
the reaction tank is further fixed by adopting a plastic PEEK screw, and the PEEK screw enables the amplification curve of fluorescent PCR to be consistent for a long time.
3. The fluorescent quantitative PCR amplification thermal cycle module of claim 1, wherein the refrigeration chip further comprises a hot side (12) and a cold side (13), the hot side (12) is in contact with the reaction cell (3), heat is absorbed from the reaction cell (3) by heat conduction, after the hot side (12) absorbs the heat, the heat is transferred from the hot side to the cold side (13) by heat conduction, and the cold side (13) is in contact with the heat sink (5) so as to rapidly emit the heat into the surrounding environment.
4. The fluorescent quantitative PCR amplification thermal cycle module as set forth in claim 3, further comprising a press block (1) and a temperature sensor (2), the temperature sensor (2) being disposed on an upper side of the reaction tank (3), the press block (1) being disposed on an upper side of the temperature sensor (2) for fixing the temperature sensor (2) and the reaction tank (3).
5. The fluorescent quantitative PCR amplification thermal cycle module of claim 1, wherein the heat sink (5) comprises a fan mount (6), a fan (7) and a fan guard (8); the fan bracket (6) is arranged on the outer side of the fan (7) and used for fixing the fan (7), and the fan screen (8) is arranged on the lower side of the fan (7) and used for protecting the fan (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310877962.4A CN116925912A (en) | 2023-07-18 | 2023-07-18 | Fluorescent quantitative PCR amplification thermal cycle module |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310877962.4A CN116925912A (en) | 2023-07-18 | 2023-07-18 | Fluorescent quantitative PCR amplification thermal cycle module |
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| Publication Number | Publication Date |
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| CN116925912A true CN116925912A (en) | 2023-10-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310877962.4A Pending CN116925912A (en) | 2023-07-18 | 2023-07-18 | Fluorescent quantitative PCR amplification thermal cycle module |
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2023
- 2023-07-18 CN CN202310877962.4A patent/CN116925912A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005117987A (en) * | 2003-10-17 | 2005-05-12 | Thermogen Kk | Device for amplifying dna |
| CN101534624A (en) * | 2008-03-14 | 2009-09-16 | 富准精密工业(深圳)有限公司 | Radiator combination |
| CN201514074U (en) * | 2009-05-18 | 2010-06-23 | 厦门海库电子有限公司 | High-efficiency large-size semiconductor refrigeration device structure |
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