CN116762632A - Tricholoma giganteum cultivation method - Google Patents
Tricholoma giganteum cultivation method Download PDFInfo
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- CN116762632A CN116762632A CN202310684402.7A CN202310684402A CN116762632A CN 116762632 A CN116762632 A CN 116762632A CN 202310684402 A CN202310684402 A CN 202310684402A CN 116762632 A CN116762632 A CN 116762632A
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- 241000302587 Macrocybe gigantea Species 0.000 title claims abstract description 46
- 238000012364 cultivation method Methods 0.000 title claims abstract description 9
- 241000233866 Fungi Species 0.000 claims abstract description 54
- 238000005286 illumination Methods 0.000 claims abstract description 26
- 239000000463 material Substances 0.000 claims abstract description 19
- 238000001816 cooling Methods 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 238000003306 harvesting Methods 0.000 claims abstract description 10
- 238000012258 culturing Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 38
- 238000003756 stirring Methods 0.000 claims description 34
- 239000002994 raw material Substances 0.000 claims description 28
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 22
- 238000011081 inoculation Methods 0.000 claims description 20
- 239000002689 soil Substances 0.000 claims description 20
- 238000012360 testing method Methods 0.000 claims description 16
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000005303 weighing Methods 0.000 claims description 9
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 claims 1
- 239000002054 inoculum Substances 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 abstract description 12
- 229920001282 polysaccharide Polymers 0.000 abstract description 12
- 239000005017 polysaccharide Substances 0.000 abstract description 12
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract description 9
- 235000013922 glutamic acid Nutrition 0.000 abstract description 9
- 239000004220 glutamic acid Substances 0.000 abstract description 9
- 238000007726 management method Methods 0.000 abstract description 9
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 abstract description 8
- 230000009418 agronomic effect Effects 0.000 abstract description 7
- 238000009776 industrial production Methods 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000011282 treatment Methods 0.000 description 11
- 230000012010 growth Effects 0.000 description 8
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 241001112361 Leucocalocybe mongolica Species 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000121219 Tricholoma Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 240000008397 Ganoderma lucidum Species 0.000 description 2
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 2
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 2
- 240000001462 Pleurotus ostreatus Species 0.000 description 2
- 241000272503 Sparassis radicata Species 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000004745 nonwoven fabric Substances 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000033962 Fontaine progeroid syndrome Diseases 0.000 description 1
- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- 206010034018 Parosmia Diseases 0.000 description 1
- 241000121220 Tricholoma matsutake Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 239000010720 hydraulic oil Substances 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 230000000258 photobiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000019525 primary metabolic process Effects 0.000 description 1
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- 238000007619 statistical method Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to the technical field of tricholoma giganteum cultivation, in particular to a tricholoma giganteum cultivation method which comprises the steps of preparing cultivation materials, bagging, sterilizing, cooling, inoculating, mycelium culturing, mycelium after-ripening, bagging and earthing, primordium forming, fruiting and management; the optimal illumination mode of tricholoma giganteum cultivation is determined through analysis of the forming time, agronomic characters (fungus thickness, fungus cover diameter, fungus handle length, fungus handle diameter and fruiting body color), harvesting time, biological efficiency, average single-bag yield of fruiting bodies and functional components (glutamic acid and polysaccharide) of the tricholoma giganteum, the optimal light source configuration of the tricholoma giganteum is found out, more scientific cultivation management measures are provided for industrial production, energy loss in cultivation is reduced, and economic and ecological benefits of enterprises are improved.
Description
Technical Field
The invention relates to the technical field of tricholoma giganteum cultivation, in particular to a tricholoma giganteum cultivation method.
Background
The tricholoma giganteum, the tricholoma giganteum and the tricholoma giganteum are high in glutamic acid content, delicious in taste, multiple in physiological functions, high in economic value and medicinal value, not easy to brown stain and rot, good in storage and transportation resistance, and free of color change and smell change after being stored for 30 days at the temperature of 8-12 ℃, are rare edible and medicinal fungi with huge market potential, the LED light source has important influence on the growth of edible fungi hyphae and fruiting bodies, and the edible fungi are generally subjected to hyphae dim light culture and fruiting body white light culture in the production aspect of the edible fungi for a long time. In recent years, students begin to study the effect of light on edible fungi, at present, the effect of light is reported in the aspects of mycelium growth and yield quality of edible fungi such as ganoderma lucidum, sparassis crispa, and red oyster mushroom, but is not reported on tricholoma giganteum, and the project provides important basis for the industrial production of tricholoma giganteum according to the primordium formation time, agronomic characters (fungus flesh thickness, fungus cover diameter, fungus handle length, fungus handle diameter, fruiting body color), harvesting time, biological efficiency, average single-bag yield of fruiting bodies, functional components (glutamic acid and polysaccharide) and the like, by testing, a light source is firstly screened out, then the optimal illumination time is determined, finally the optimal light source configuration suitable for the growth of the tricholoma giganteum is found, and a set of illumination facility cultivation scheme of the tricholoma giganteum is optimized.
Disclosure of Invention
The invention aims to provide a tricholoma giganteum cultivation method for solving the problems in the background technology. In order to achieve the above purpose, the present invention provides the following technical solutions: a tricholoma giganteum cultivation method, the cultivation comprising the steps of:
1) Preparing a cultivation material: weighing cultivation raw materials according to the weight ratio, weighing the cultivation raw materials with equal parts by weight, then concentrating the cultivation raw materials into a raw material vessel, adding water and uniformly stirring by a stirrer;
10 Testing; firstly weighing the weight of a test cultivation raw material, then adding water and lime water into a measuring cup respectively, finally pouring the water in the measuring cup into a stirrer for fully stirring to fully absorb water, then adding a proper amount of lime water for uniformly stirring to ensure that the pH value is 8.0-9.0, and then recording consumption values of the water, the lime water and the cultivation raw material in the measuring cup;
11 Preparing; according to the weight of the cultivation raw materials, corresponding water and lime water are prepared correspondingly according to the test value;
12 Stirring; placing the cultivation raw materials, water and lime water into a stirrer for stirring, and ensuring the pH value to be 8.5 after stirring.
2) The water content of the culture material is regulated to 60-70%, and pH test paper is used to test the pH value of the culture material to 8.0-9.0.
3) And (3) sterilization: sterilizing to aseptic state.
4) And (3) cooling: after sterilization, the bottles are manually placed on the holding tray in a concentrated mode and then are sequentially moved into the cooling chamber.
5) Inoculating: and inoculating about 18 bags to 22 bags of cultivated species to each bottle of strain, and manually checking the inoculation amount of each bottle of strain in sequence to ensure the consistency of the inoculation amount.
6) Hypha culture: after inoculation, the cultivars are manually placed in sequence and transferred into a strain culture room for culture through a cart.
7) Post-ripening of hyphae: after inoculation is finished, culturing is carried out for 43-47 days, and a culture staff can check whether the hyphae grow into bags or not, and whether the standards of the bags are in accordance with the requirements of hyphae after-ripening or not.
8) Opening a bag and covering soil: and (5) manually and sequentially opening the post-matured fungus bags, and then covering soil, so that the fungus bags are prevented from being damaged in the soil covering process.
9) Primordium formation: and (3) forming primordia about 23 to 27 days after opening the bag and covering the soil (preferably about 25 days after opening the bag and covering the soil), and carrying out fruiting and management.
10 Fruiting and management: harvesting is carried out about 8 days to 12 days after primordia are formed (10 days after optimal primordia are formed); the LED is a cold light source, has small heat dissipation capacity, and can be irradiated in a short distance in the production of edible fungi without causing a large amount of water loss on the surface of the fungus stick; the light quality, the light intensity and the light period of the LED light source can be regulated and controlled, so that the study on the photobiological characteristics of the growth and development of the edible fungi is facilitated, the study on the different light qualities of the LED light source in the aspect of edible fungi is reported in the aspects of the growth and the yield quality of hyphae such as ganoderma lucidum, sparassis crispa, red oyster mushroom and the like, the best light source configuration suitable for the growth of the tricholoma giganteum is found out, the energy is saved, and the method is a study direction of a tricholoma giganteum factory.
Currently, the tricholoma giganteum industry has the following problems to be solved urgently, the influence of light on the tricholoma giganteum is not supported by data, and a scientific management method and management technology are not formed; the research adopts different light sources and illumination time to treat, researches the influence of the light sources and illumination time on the formation time, the harvesting time, the agronomic characters (fungus flesh thickness, fungus cover diameter, fungus handle length, fungus handle diameter and fruiting body color) of the tricholoma giganteum, biological efficiency, average single-bag yield of the fruiting body and functional components thereof, finds out the optimal light source configuration suitable for the growth of the tricholoma giganteum, determines the optimal illumination mode of the cultivation of the tricholoma giganteum, and provides basis for the industrial production of the tricholoma giganteum.
Preferably, when bagging, 17cm by 34cm bags are used for bagging, the middle part is punched to the bottom of the bottle, and the cover is closed.
Preferably, during cooling, the temperature of the cultivation material is moved into a cooling chamber to be cooled to about 28 ℃ to 32 ℃ (the optimal temperature is about 30 ℃).
Preferably, the inoculation is performed without missing any connection, and all relevant equipment, working tools, appliances and hands (or gloves) which are prevented from being polluted during the inoculation process are sterilized and then inoculated.
Preferably, the strain chamber is kept clean during mycelium culture, and the temperature is controlled at 25 ℃ to 31 ℃ (the optimal temperature is 28 ℃).
Preferably, when the mycelium is after-matured, the mycelium needs to be kept for 42 to 47 days (the optimal placing time is 45 days) after being fully packed.
Preferably, when the bag is opened and covered with soil, the fungus bag is sprayed with water after the soil is covered, and the fungus bag is kept moist.
Preferably, during fruiting and management, conditions such as temperature, carbon dioxide concentration, illumination and the like of a fruiting room are regulated during the period, an illumination experiment result is adopted, the nutrition component is highest and the yield is highest in 8 hours of green illumination, the tricholoma giganteum for the research is a tricholoma giganteum strain collected and independently identified in Sanming sha county, and NCBI is submitted to obtain GeneBank number MW192277 and named as tricholoma giganteum (K-1); (1) The mycelium of Tricholoma lobayense (K-1) is respectively subjected to 5 treatments of red light (R), blue light (B), green light (G), yellow light (Y) and white light (W), the illumination intensity is 100PPFD, the darkness condition is set as a contrast (the study is called different light quality treatment), the distance between a light source and a culture material is kept at 50cm, and the outer layer is covered by black non-woven fabrics; (2) The mycelium illumination treatment period is full illumination, half a day and half a day, and one day and half a day; (3) The appearance of the mycelium after different light treatments was analyzed: mycelium was measured for colony diameter by the "crisscross method" from day 4, 1 time every 3 days, to the end of day 14, colony morphology was observed and recorded, 10 plates were used as one treatment, and each treatment was repeated 3 times; (4) Taking a Tricholoma lobayense (K-1) fungus bag in the same growth period, opening the bag, covering soil, respectively placing in 4 treatments of red light (R), blue light (B), green light (G) and yellow light (Y), wherein the illumination intensity is 150 lux, and white light is used as a control (the study is called different light quality treatment); the distance between the light source and the material surface is kept 50cm, the outer layer is covered by black non-woven fabrics, 10 bags are used as one treatment, each treatment is repeated for 3 times, the whole-day illumination treatment is carried out on the light source, the primordium formation time is observed and recorded, after the mushroom fruiting is carried out, the harvesting time and the agronomic characters (fungus meat thickness, fungus cover diameter, fungus handle length, fungus handle diameter and fungus cover color) are recorded, the average single bag yield and the biological efficiency are measured in the same harvesting period, and the illumination duration of the single light source is optimized after the optimal light source is selected according to test recording indexes, wherein the duration is set as follows: full illumination, 8 hours, 4 hours and 2 hours to optimize the optimal light source configuration; (5) Statistical analysis test data are analyzed and processed by using statistical software SPSS statics 17.0, and differences among the processes are checked by adopting a Duncan new complex pole difference method in single-factor variance analysis multiple comparison; 2. the polysaccharide and glutamic acid content analysis light of the mycelium and fruiting body of the Tricholoma lobayense (K-1) under different illumination conditions not only affects the appearance and yield of the edible fungi, but also has a certain regulation and control effect on the primary and secondary metabolism of the edible fungi; on the basis of scheme 1, selecting strains with high quality and obvious yield difference of agronomic characters (fungus meat thickness, fungus cover diameter, fungus handle length, fungus handle diameter and fungus cover color) of the Tricholoma matsutake (K-1) under different illumination and different durations, analyzing the content of metabolite polysaccharide and glutamic acid, wherein the research scheme is as follows: (1) The polysaccharide content detection is carried out by using a polysaccharide test box, the polysaccharide can react with a color reagent to generate a complex, and the light absorption value is measured at 540nm by using a full-automatic enzyme-labeled instrument to calculate the content of the polysaccharide; (2) this item is intended to be tested using GCMS; polysaccharide and glutamic acid are important manifestations of taste, nutritional value and functional components of the tricholoma mongolicum (K-1), glutamic acid and polysaccharide contents under different illumination and time periods are analyzed, the results are ranked, illumination configurations most suitable for accumulation of the nutrition components of the tricholoma mongolicum (K-1) and the functional components of the tricholoma mongolicum are screened, and important theoretical basis is provided for cultivation of the tricholoma mongolicum (K-1) with good quality in the future.
The primary objective of the project is to determine the optimal illumination mode of tricholoma giganteum cultivation by analyzing the forming time, agronomic characters (fungus thickness, fungus cover diameter, fungus handle length, fungus handle diameter and fruiting body color), harvesting time, biological efficiency, average single-bag yield of fruiting bodies and functional components (glutamic acid and polysaccharide) thereof of the tricholoma giganteum primordium, find out the optimal light source configuration of the tricholoma giganteum, provide more scientific cultivation management measures for industrial production, reduce energy loss in cultivation and improve economic and ecological benefits of enterprises.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the optimal illumination mode of tricholoma giganteum cultivation is determined through analysis of the forming time, agronomic characters (fungus thickness, fungus cover diameter, fungus handle length, fungus handle diameter and fruiting body color), harvesting time, biological efficiency, average single-bag yield of fruiting bodies and functional components (glutamic acid and polysaccharide) of the tricholoma giganteum, the optimal light source configuration of the tricholoma giganteum is found, more scientific cultivation management measures are provided for industrial production, energy loss in cultivation is reduced, and economic and ecological benefits of enterprises are improved.
According to the invention, the numerical value obtained through the test in the cultivation material is prepared, the corresponding water and lime water (such as clarified lime water) are weighed through the measuring cup, and then the pH value of the cultivation raw material is quickly close to 8.5 in the process of stirring the cultivation raw material, namely the defect of low efficiency of the stirring machine that the pH value is 8.5 after repeatedly adding water and lime water (such as clarified lime water) for a plurality of times in the stirring process is avoided; simultaneously, the motor drives the U-shaped stirring rod to stir with the stirrer together, so that the stirring efficiency of the stirrer is improved rapidly.
Drawings
FIG. 1 is a schematic flow chart of a method for cultivating tricholoma giganteum in the invention;
FIG. 2 is a schematic diagram of a process structure for preparing a cultivation material in the invention;
FIG. 3 is a schematic diagram of the structure of an explosion with stirring in the preparation of a cultivation material in the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which are obtained by a worker of ordinary skill in the art without creative efforts, are within the protection scope of the present invention based on the embodiments of the present invention.
Embodiment one;
referring to fig. 1 to 3, the present invention provides a technical solution: a tricholoma giganteum cultivation method, the cultivation comprising the steps of:
1) Preparing a cultivation material: weighing cultivation raw materials according to the weight ratio, weighing the cultivation raw materials with equal parts by weight, then concentrating the cultivation raw materials into a raw material vessel, adding water and uniformly stirring by a stirrer.
2) Bagging: the water content of the culture medium is adjusted to 60-70% (optimal water content 65%), and pH value is adjusted to 8.0-9.0 (optimal pH value is 8.5) by using pH test paper.
3) And (3) sterilization: sterilizing to aseptic state. The high pressure sterilization method is adopted to keep the temperature of 121 ℃ for 1.8 to 2.2 hours, the ultra-high pressure sterilization technology is adopted, namely, materials such as flexible package food and the like are applied with 400 to 600MPa pressure or 100 to 1000MPa pressure is applied with high-grade hydraulic oil by using water as medium in a closed ultra-high pressure container, so that almost all bacteria in the materials are killed, and the optimal high pressure sterilization method is kept for 2 hours at 121 ℃ until the materials reach a sterile state.
4) And (3) cooling: after sterilization, the bottles are manually placed on the holding tray in a concentrated mode and then are sequentially moved into the cooling chamber.
5) Inoculating: and inoculating about 18 bags to 22 bags (optimal 20 bags) of strains to each bottle of strain for cultivating the strain, and manually checking the inoculation amount of each bottle of strain in sequence to ensure the consistency of the inoculation amount.
6) Hypha culture: after inoculation, the cultivars are manually placed in sequence and transferred into a strain culture room for culture through a cart.
7) Post-ripening of hyphae: after inoculation is finished, culturing is carried out for 43-47 days (optimal culturing is carried out for 45 days), and a culture staff can check whether hyphae grow into bags or not, and whether the standard of the bags growing into the bags meets the requirement of hyphae after-ripening or not.
8) Opening a bag and covering soil: and (5) manually and sequentially opening the post-matured fungus bags, and then covering soil, so that the fungus bags are prevented from being damaged in the soil covering process.
9) Primordium formation: and (3) forming primordia about 23 to 27 days after opening the bag and covering the soil (preferably about 25 days after opening the bag and covering the soil), and carrying out fruiting and management.
10 Fruiting and management: harvesting is carried out about 8 days to 12 days after primordia are formed (about 10 days after optimal primordia are formed).
In this example, 17cm by 34cm bags were used to fill bags, the middle was perforated to the bottom of the bottle, and the lid was closed.
In this example, during cooling, the temperature of the cultivation material is moved into a cooling chamber to be cooled to about 28 ℃ to 32 ℃ (the optimum temperature is 30 ℃).
In this embodiment, during inoculation, all relevant equipment, tools and hands (or gloves) must be sterilized during inoculation without missing the inoculation, and then inoculation is performed.
In this example, the strain chamber was kept clean during mycelium cultivation, and the temperature was controlled at 25℃to 31℃with an optimum temperature of 28 ℃.
In this example, when the mycelium is after-matured, the mycelium should be kept for 42 to 47 days (the optimal time for the bag is 45 days) after-maturing.
In the embodiment, when the bag is opened and soil is covered, water is sprayed to the fungus bag after the soil is covered, and the fungus bag is kept moist.
Embodiment two;
the first embodiment is basically the same as the first embodiment, and the difference between the first embodiment is that referring to fig. 1 to 3, the present invention provides a technical scheme: a tricholoma giganteum cultivation method, wherein the step 1) is to prepare cultivation materials: weighing cultivation raw materials according to the weight portion, adding water and uniformly stirring, and specifically comprises the following steps:
10 Testing; firstly weighing the weight of a test cultivation raw material, then adding water and lime water (such as clarified lime water) into a measuring cup respectively, finally pouring the water in the measuring cup into a stirrer for fully stirring to enable the water to fully absorb water, then adding a proper amount of lime water for uniformly stirring to enable the pH value to be 8.5, and then recording the water in the measuring cup, the lime water (such as clarified lime water) and consumption values of each group of cultivation raw materials;
11 Preparing; according to the weight of the cultivation raw materials, corresponding water and lime water (such as clarified lime water) are prepared correspondingly according to the test value;
12 Stirring; placing the cultivation raw materials, water and lime water into a stirrer for stirring, and ensuring the pH value of the cultivation raw materials to be 8.5 after stirring; the specific stirring steps are as follows: pouring the cultivation raw materials into a stirrer, pouring water in a measuring cup into the stirrer through a water inlet, and then externally connecting a power supply to a motor to start a switch, so that the motor drives a U-shaped stirring rod to rotate, and the U-shaped stirring rod and the stirring rod in the stirrer reversely rotate, thereby achieving the purpose of rapid stirring and accelerating the stirring efficiency of the original stirrer; the quantitative lime water (such as clarified lime water) added into the measuring cup is poured into the stirrer to enable the pH value to be 8.5, namely the defect that the stirring efficiency of the stirrer is low when the stirring process is repeated for adding water and lime water (such as clarified lime water) for a plurality of times, and the pH value is 8.5 is avoided.
In this embodiment, during fruiting and management, conditions such as temperature, carbon dioxide concentration, illumination and the like of a fruiting room are adjusted during this period, and the result of illumination experiments adopts that the nutrient content is highest and the yield is highest in 8 hours of green illumination.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present invention, and are not intended to limit the invention, and that various changes and modifications may be made therein without departing from the spirit and scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A tricholoma giganteum cultivation method is characterized in that: the cultivation comprises the following steps:
1) Preparing a cultivation material: weighing cultivation raw materials according to the weight portion, adding water and uniformly stirring;
10 Testing; firstly weighing the weight of the experimental cultivation raw materials, pouring water in a measuring cup into a stirrer through a water inlet, finally pouring the water in the measuring cup into the stirrer for fully stirring to fully absorb water, adding a proper amount of lime water for uniformly stirring to enable the pH value to be 8.5, and recording consumption values of the water, the lime water and the cultivation raw materials in the measuring cup;
11 Preparing; according to the weight of the cultivation raw materials, corresponding water and lime water are prepared correspondingly according to the test value;
12 Stirring; placing the cultivation raw materials, water and lime water into a stirrer for stirring, and ensuring the pH value of the cultivation raw materials to be 8.5 after stirring;
2) Bagging: the water content of the culture material is adjusted to 65 percent, so that the pH value of the culture material is 8.5;
3) And (3) sterilization: sterilizing to aseptic state;
4) And (3) cooling: after sterilization, the bottle is moved into a cooling chamber;
5) Inoculating: inoculating about 20 bags of cultivated species to each bottle of strain, and ensuring the consistent inoculum size;
6) Hypha culture: transferring the strain into a strain culture chamber for culture after inoculation;
7) Post-ripening of hyphae: culturing for 45 days after inoculation, and allowing mycelia to grow into bags;
8) Opening a bag and covering soil: wrapping the post-ripe fungus with a bag and then covering with soil;
9) Primordium formation: forming primordia about 25 days after opening the bag and covering soil;
10 Fruiting and management: harvesting can be performed about 10 days after primordia are formed.
2. The method for cultivating tricholoma giganteum according to claim 1, wherein: when in bagging, a 17cm x 34cm bag is used for bagging, the middle part is perforated to the bottom of the bottle, and the cover is tightly covered.
3. The method for cultivating tricholoma giganteum according to claim 1, wherein: during cooling, the temperature of the cultivation raw material is moved into a cooling chamber to be cooled to about 30 ℃.
4. The method for cultivating tricholoma giganteum according to claim 1, wherein: during inoculation, the inoculation is not missed, all relevant equipment, operation tools, appliances and hand whiskers are prevented from being disinfected under the pollution condition in the inoculation process, and then the inoculation is carried out.
5. The method for cultivating tricholoma giganteum according to claim 1, wherein: when mycelium is cultured, the strain chamber is kept clean, and the temperature is controlled at 28 ℃.
6. The method for cultivating tricholoma giganteum according to claim 1, wherein: when the mycelium is after-ripened, the mycelium needs to be continuously placed for 45 days after being fully bagged for after-ripening.
7. The method for cultivating tricholoma giganteum according to claim 1, wherein: when the bag is opened and covered with soil, the fungus bag is sprayed with water after the soil is covered, and the fungus bag is kept moist.
8. The method for cultivating tricholoma giganteum according to claim 1, wherein: during fruiting and management, the illumination condition of a fruiting room is adjusted during the fruiting period, and green light is adopted to illuminate for 8 hours, so that the nutritional ingredients are highest, and the yield is highest.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310684402.7A CN116762632A (en) | 2023-06-09 | 2023-06-09 | Tricholoma giganteum cultivation method |
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