CN116769598A - In-vitro culture device for artificial tissue - Google Patents
In-vitro culture device for artificial tissue Download PDFInfo
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- CN116769598A CN116769598A CN202310904232.9A CN202310904232A CN116769598A CN 116769598 A CN116769598 A CN 116769598A CN 202310904232 A CN202310904232 A CN 202310904232A CN 116769598 A CN116769598 A CN 116769598A
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- artificial tissue
- vitro culture
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- 238000000338 in vitro Methods 0.000 title claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 133
- 238000007789 sealing Methods 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 5
- 210000004204 blood vessel Anatomy 0.000 abstract description 22
- 238000004113 cell culture Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 7
- 230000004087 circulation Effects 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 238000009987 spinning Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000010349 pulsation Effects 0.000 description 2
- 239000002473 artificial blood Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 210000005167 vascular cell Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application discloses an in-vitro culture device for artificial tissues, which comprises an incubator main body with a containing cavity and an autorotation liquid pipe, wherein a culture bracket is arranged on the periphery of the autorotation liquid pipe in the incubator main body, the autorotation liquid pipe is arranged in a hollow mode, the autorotation liquid pipe is rotatably connected to the incubator main body, and when liquid is pumped into the autorotation liquid pipe in a unidirectional mode, the autorotation liquid pipe rotates; the application has simple structure and can improve the uniformity of blood vessels after cell culture.
Description
Technical Field
The application relates to the technical field of vascular culture, in particular to an in-vitro culture device for artificial tissues.
Background
In the traditional artificial tissue culture device, the bracket tissue attached with cells is directly cultured in a culture container, and when nutrient solution is needed to be replaced, the nutrient solution is pumped out for replacement. In the whole culture process, external stirring is difficult to apply, so that cell secretion in a culture container and various nutrients in a culture solution are difficult to uniformly distribute, tissue growth is inhibited, and the culture solution and various nutrients are wasted.
In the conventional art, an artificial blood vessel culturing apparatus includes an incubator body in which a blood vessel is cultured, both ends of the blood vessel are fixed, so that the blood vessel maintains a tubular structure, and is immersed in a culture solution, a device (a common tube (in the blood vessel)) which plays a role in fixing both ends of the blood vessel is inserted into the inside of both ends of the blood vessel, and then one end of the blood vessel is bound to the fixing device using a wire, a band, or the like, so that the blood vessel is fixed. The fixing device is a tubular hollow structure, and is communicated with the inside of the blood vessel and the outside of the culture container, so that external liquid can be introduced into the device, and the previous liquid in the tubular liquid can be pumped out. In such a device, the vessel is placed in a substantially stationary state in the culture vessel, and the culture solution in the culture vessel is also placed in a stationary state, and during the culture, cells accumulate toward the bottom of the vessel stent due to gravity, resulting in a wall thickness at the bottom of the vessel that is greater than the wall thickness at the top, failing to satisfy the uniformity of the vessel. In order to solve the technical problem of poor uniformity of blood vessels, in the prior art, an incubator body is placed on a shaker or the like, for example, under the name of "incubator apparatus and method of using the same", publication number CN115820420 a, publication date 2023.03.21, which includes an incubator body, a first pipe assembly, a second pipe assembly, and a movement assembly. A bearing plate is movably arranged in the accommodating space of the incubator main body, a sterile culture bag is arranged on the bearing plate, and a base tube and a culture bracket sleeved on the periphery of the base tube are arranged in a bag cavity of the sterile culture bag; the first pipeline component is internally provided with a first circulation loop for updating the culture solution, and is also provided with a second pipeline component for simulating the dynamic environment of blood flow in a human body so as to obtain tissue engineering blood vessels which are more similar to the tissue structure and function of the blood vessels of the human body; the second circuit assembly has a second circulation loop therein, and after the second circuit assembly pumps the second liquid into the base pipe, the second liquid periodically expands and compresses the base pipe, thereby causing the base pipe to pulsate. Finally, the bearing plate moves according to the preset requirement through the movement assembly, so that the vascular cells are in full contact with the nutrient solution. The technical scheme adopts two circulation loops and one motion assembly, and relates to a plurality of power sources, which has large integral volume, complex structure, high manufacturing cost and difficult maintenance and repair.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.
The present application has been made in view of the above and/or the problems of vessel culture in the prior art.
The application solves the technical problems of complex structure and high manufacturing cost.
Therefore, the application aims to provide an in vitro culture device for artificial tissues, which has a simple structure, can enable cells to fully contact with nutrient solution, and can improve the uniformity of blood vessels after cell culture.
In order to solve the technical problems, the application provides the following technical scheme: the utility model provides an external culture apparatus of artificial tissue, its includes incubator main part and rotation liquid pipe that has the holding chamber, rotation liquid pipe periphery in the incubator main part is equipped with the culture bracket, rotation liquid pipe is inside cavity setting, rotation liquid pipe rotationally connects on the incubator main part, when the unidirectional pump was gone into liquid in the rotation liquid pipe, rotation liquid pipe rotated.
As a preferred embodiment of the in vitro culture apparatus for artificial tissue according to the application, wherein: the inner side of the autorotation liquid pipe is provided with a plurality of bulges, one side of each bulge is fixed on the inner side of the autorotation liquid pipe, and the other side of each bulge is inclined towards the flowing direction of liquid.
As a preferred embodiment of the in vitro culture apparatus for artificial tissue according to the application, wherein: and a spiral sinking groove is formed in the inner side of the autorotation liquid pipe.
As a preferred embodiment of the in vitro culture apparatus for artificial tissue according to the application, wherein: the two ends of the autorotation liquid pipe are rotatably connected with the two ends of the incubator main body through support bearings.
As a preferred embodiment of the in vitro culture apparatus for artificial tissue according to the application, wherein: the outside at incubator main part both ends is connected with the feed liquor pipe that is used for the feed liquor respectively and is used for the drain pipe of play liquid, feed liquor pipe and drain pipe inside all communicate with rotation liquid intraductal.
As a preferred embodiment of the in vitro culture apparatus for artificial tissue according to the application, wherein: the liquid inlet pipe, the liquid outlet pipe and the incubator body are integrated, and the inner diameters of the liquid inlet pipe and the liquid outlet pipe are not smaller than the outer diameter of the support bearing.
As a preferred embodiment of the in vitro culture apparatus for artificial tissue according to the application, wherein: the liquid inlet pipe and the liquid outlet pipe are detachably connected to the incubator body.
As a preferred embodiment of the in vitro culture apparatus for artificial tissue according to the application, wherein: the one end that the feed liquor pipe set up relative incubator main part is fixed with the thread bush, the one side outwards of incubator main part both ends all opens and connects heavy groove, and the thread bush is connected heavy groove threaded connection on the incubator main part, and the internal diameter of thread bush is greater than the external diameter of support bearing.
As a preferred embodiment of the in vitro culture apparatus for artificial tissue according to the application, wherein: the inner side of the liquid inlet pipe opposite to the incubator main body at one end is fixed with a sealing step extending towards the direction far away from the incubator main body, the sealing step covers the support bearing, and when the liquid inlet pipe is abutted to the outer side of the incubator main body, the sealing piece is abutted to the outer side of the support bearing.
Compared with the prior art, the application has the advantages that: only the liquid is pumped into the autorotation liquid pipe continuously, the autorotation liquid pipe rotates to drive the bracket to rotate, so that cells attached to blood vessels are uniform, the cultured blood vessels are uniform in structure, and meanwhile, the culture medium around the bracket is stirred, so that factors and cell secretions in the culture medium are uniformly distributed; the application has simple structure, replaces complex structure in the prior art, and achieves better technical effect.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
fig. 1 is a perspective view of the present application.
Fig. 2 is a top view of the present application.
Fig. 3 is a view from the side of fig. 2 at A-A (an internal structural view of the present application).
Fig. 4 is another internal structural view of the present application.
Fig. 5 is a perspective view of a first embodiment of the rotation liquid pipe according to the present application.
Fig. 6 is a second perspective view of the rotation liquid pipe in the present application.
In the figure, the incubator body 100, the liquid outlet pipe 200, the threaded sleeve 201, the sealing step 202, the blood vessel 300, the liquid inlet pipe 400, the supporting bearing 500, the protrusion 600, the rotation liquid pipe 700, the spiral sinking groove 701 and the liquid seepage hole 702 are arranged.
Detailed Description
In order that the above-recited objects, features and advantages of the present application will become more readily apparent, a more particular description of the application will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, but the present application may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present application is not limited to the specific embodiments disclosed below.
Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the application. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Referring to fig. 1 to 3, the present embodiment provides an in vitro culture apparatus for artificial tissue, which has a simple structure and more uniform distribution of blood vessels 300 after culture.
The utility model provides an external culture apparatus of artificial tissue, its includes incubator main part 100 and rotation liquid pipe 700 that has the chamber that holds, rotation liquid pipe 700 periphery in the incubator main part 100 is equipped with the culture bracket, rotation liquid pipe 700 is inside cavity setting, rotation liquid pipe 700 rotationally connects on incubator main part 100, when the unidirectional pumping liquid in to rotation liquid pipe 700 inside, rotation liquid pipe 700 rotates.
Specifically, a plurality of protrusions 600 are arranged on the inner side of the rotation liquid pipe 700, one side of each protrusion 600 is fixed on the inner side of the rotation liquid pipe 700, and the other side of each protrusion 600 is inclined towards the liquid flowing direction.
Specifically, the outer sides of the two ends of the incubator main body 100 are respectively connected with a liquid inlet pipe 400 for liquid inlet and a liquid outlet pipe 200 for liquid outlet, and the interiors of the liquid inlet pipe 400 and the liquid outlet pipe 200 are communicated with the interior of the autorotation liquid pipe 700.
In implementation, one end of the liquid inlet pipe 400 and the liquid outlet pipe 200, which are far away from the incubator main body 100, is respectively connected with a first pipeline and a second pipeline, the first pipeline is connected with a liquid pump, one end of the liquid pump, which is far away from the first pipeline, is connected with a liquid storage tank, one end of the second pipeline, which is far away from the incubator main body 100, is connected with the liquid storage tank, and the flexible bracket is wound on the periphery of the autorotation liquid pipe 700; when the device works, the liquid pump pumps the liquid in the liquid storage tank out, the liquid is pumped into the autorotation liquid pipe 700 through the liquid inlet pipe 400, the power of heart pulsation is simulated, the liquid is contacted with the bulge 600, the force for rotating the autorotation liquid pipe 700 is shunted, the autorotation liquid pipe 700 drives the bracket to rotate, so that cells attached to the blood vessel 300 become uniform, the cultured blood vessel 300 is uniform in structure, and meanwhile, the culture medium around the bracket is stirred, so that factors and cell secretion in the culture medium are uniformly distributed; the application is more stable, simple and efficient.
Specifically, the liquid inlet pipe 400, the liquid outlet pipe 200 and the incubator body 100 are integrally formed, the inner diameters of the liquid inlet pipe 400 and the liquid outlet pipe 200 are not smaller than the outer diameter of the support bearing 500, and in this embodiment, the inner diameters of the liquid inlet pipe 400 and the liquid outlet pipe 200 are larger than the outer diameter of the support bearing 500.
During installation, two support bearings 500 are installed at two ends of the autorotation liquid pipe 700 in the axial direction, and the autorotation liquid pipe 700 is inserted into the liquid inlet pipe 400 and then sequentially pressed into connecting holes formed at two ends of the incubator main body 100.
Example 2
Referring to fig. 5, the present embodiment provides an in vitro culture apparatus for artificial tissue, which is based on the above embodiment, and which enables rotation of a stent when liquid is pumped into a spinning liquid tube 700.
Specifically, a plurality of spiral sink grooves 701 are arranged on the inner side of the self-rotation liquid pipe 700.
Liquid is pumped into the spinning liquid pipe 700, and the liquid contacts the spiral settling tank 701, and the spinning liquid pipe 700 rotates.
Example 3
Referring to fig. 4, the present embodiment provides an in vitro culture apparatus for artificial tissue, which is based on the above two embodiments, and can further facilitate the connection of the liquid inlet tube 400, the liquid outlet tube 200 and the incubator body 100.
Specifically, the feed liquor pipe 400 and the drain pipe 200 are all detachably connected on the incubator main body 100, one end of the feed liquor pipe 400, which is arranged relative to the incubator main body 100, is fixed with a thread bush 201, one outward side of two ends of the incubator main body 100 is provided with an annular connection sink, the thread bush 201 is connected on the incubator main body 100 through the connection sink thread, and the inner diameter of the thread bush 201 is larger than the outer diameter of the support bearing 500.
The liquid outlet pipe 200 and the liquid inlet pipe 400 have the same structure, and by taking the installation of the liquid inlet pipe 400 as an example, the threaded sleeve 201 is screwed into the connecting sink until the liquid inlet pipe 400 is abutted against the outer side of the incubator main body 100, so that the connection between the liquid inlet pipe 400 and the incubator main body 100 is realized.
The inner side of the end of the liquid inlet pipe 400 opposite to the incubator main body 100 is fixed with a sealing step 202 extending towards the direction far away from the incubator main body 100, the sealing step 202 covers the support bearing 500, and when the liquid inlet pipe 400 abuts against the outer side of the incubator main body 100, the sealing element abuts against the outer side of the support bearing 500.
When the liquid inlet pipe 400 is abutted against the outer side of the incubator main body 100, the sealing step 202 is attached to the outer side of the support bearing 500, and after liquid enters the autorotation liquid pipe 700 through the liquid inlet pipe 400, the liquid is not contacted with the support bearing 500, so that the service life of the support bearing 500 is prolonged.
Example 4
Referring to fig. 6, the present embodiment provides an in vitro culture apparatus for artificial tissue, which is based on the above three embodiments, and can further accelerate the culture efficiency.
Specifically, the autorotation liquid tube 700 is hollowed out, that is, the autorotation liquid tube 700 is provided with a plurality of liquid seepage holes 702 at the periphery of one section in the incubator main body 100, when in culture, cells at the innermost layer of the blood vessel are contacted with nutrient solution for simulating blood pulsation in the autorotation liquid tube 700 through the positions of the liquid seepage holes 702, the culture period is shortened, and the culture efficiency is further improved.
It should be noted that the above embodiments are only for illustrating the technical solution of the present application, and not for limiting the structure of the rotation liquid pipe 700, but other structures are also possible, and not limited to the three structures of the present application, and although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present application can be modified or replaced equally without departing from the spirit and scope of the technical solution of the present application, and it should be covered by the scope of the claims of the present application.
Claims (10)
1. An in vitro culture device for artificial tissue, comprising an incubator body (100) with a receiving chamber, characterized in that: also comprises;
the culture medium box comprises a self-rotation liquid pipe (700), wherein a culture support is arranged on the periphery of the self-rotation liquid pipe (700) in the culture box main body (100), the self-rotation liquid pipe (700) is arranged in a hollow mode, the self-rotation liquid pipe (700) is rotatably connected to the culture box main body (100), and when liquid is pumped into the self-rotation liquid pipe (700) in a one-way mode, the self-rotation liquid pipe (700) rotates.
2. The in vitro culture device of artificial tissue according to claim 1, wherein: a plurality of protrusions (600) are arranged on the inner side of the self-rotation liquid pipe (700), one side of each protrusion (600) is fixed on the inner side of the self-rotation liquid pipe (700), and the other side of each protrusion (600) is inclined towards the flowing direction of liquid.
3. The in vitro culture device of artificial tissue according to claim 1, wherein: a spiral sinking groove (701) is arranged on the inner side of the self-rotation liquid pipe (700).
4. An in vitro culture device for artificial tissue according to any one of claims 1 to 3, wherein: the two ends of the self-rotation liquid pipe (700) are rotatably connected with the two ends of the incubator main body (100) through the support bearings (500).
5. An in vitro culture device for artificial tissue according to any one of claims 1 to 3, wherein: the outside at incubator main part (100) both ends is connected with feed liquor pipe (400) and drain pipe (200) that are used for the feed liquor respectively, feed liquor pipe (400) and drain pipe (200) are inside all with rotation liquid pipe (700) inside intercommunication.
6. The in vitro culture device for artificial tissue according to claim 5, wherein: the liquid inlet pipe (400), the liquid outlet pipe (200) and the incubator main body (100) are integrated, and the inner diameters of the liquid inlet pipe (400) and the liquid outlet pipe (200) are not smaller than the outer diameter of the support bearing (500).
7. The in vitro culture device for artificial tissue according to claim 5, wherein: the liquid inlet pipe (400) and the liquid outlet pipe (200) are detachably connected to the incubator main body (100).
8. The in vitro culture device of artificial tissue according to claim 7, wherein: one end of the liquid inlet pipe (400) arranged relative to the incubator main body (100) is fixedly provided with a thread bush (201), one outwards-facing sides of two ends of the incubator main body (100) are provided with annular connecting sinking grooves, the thread bush (201) is connected to the incubator main body (100) through connecting sinking grooves in a threaded manner, and the inner diameter of the thread bush (201) is larger than the outer diameter of the supporting bearing (500).
9. The in vitro culture device of artificial tissue according to claim 8, wherein: the inside that feed liquor pipe (400) set up one end relative incubator main part (100) is fixed with sealed step (202) that extend towards keeping away from incubator main part (100) place direction, sealed step (202) cover support bearing (500), when feed liquor pipe (400) conflict in incubator main part (100) outside, the sealing member is contradicted in support bearing (500) outside.
10. An in vitro culture device for artificial tissue according to any one of claims 1 to 3, wherein: the autorotation liquid pipe (700) is hollow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310904232.9A CN116769598A (en) | 2023-07-24 | 2023-07-24 | In-vitro culture device for artificial tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310904232.9A CN116769598A (en) | 2023-07-24 | 2023-07-24 | In-vitro culture device for artificial tissue |
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| Publication Number | Publication Date |
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| CN116769598A true CN116769598A (en) | 2023-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310904232.9A Pending CN116769598A (en) | 2023-07-24 | 2023-07-24 | In-vitro culture device for artificial tissue |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372660A (en) * | 2008-03-18 | 2009-02-25 | 北京航空航天大学 | Pourable vascular tissue engineering reactor having rotating function |
| KR20110073949A (en) * | 2009-12-24 | 2011-06-30 | 연세대학교 산학협력단 | A method for regenerating tubelar-type tissues and organs, and thd bioreactor system |
| CN102872478A (en) * | 2012-10-19 | 2013-01-16 | 陕西正凡科技发展有限公司 | Method for constructing full-biological tissue engineering blood vessel |
| CN106264745A (en) * | 2016-08-03 | 2017-01-04 | 苏州大学附属第二医院 | Tumor-microvessel self assembly generation system |
| CN205874451U (en) * | 2016-08-03 | 2017-01-11 | 苏州大学附属第二医院 | Artificial blood vessel's cultivation and function detecting device |
| CN106591121A (en) * | 2016-11-30 | 2017-04-26 | 广州博敏科技有限公司 | Rotation culture chamber and rotation pulsation culture system |
| CN210048789U (en) * | 2019-04-01 | 2020-02-11 | 刘亮 | Artificial blood vessel endothelialization culture apparatus |
| CN111603611A (en) * | 2020-03-06 | 2020-09-01 | 广东省人民医院(广东省医学科学院) | Cell-derived matrix tubular scaffold and preparation method thereof |
| US20210222130A1 (en) * | 2018-08-07 | 2021-07-22 | 1Lab Sa | Model for in-vitro simulation of the behaviour of dysfunctional vessels |
| CN214263118U (en) * | 2020-10-30 | 2021-09-24 | 湖北华电江陵发电有限公司 | Automatic cleaning device |
-
2023
- 2023-07-24 CN CN202310904232.9A patent/CN116769598A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372660A (en) * | 2008-03-18 | 2009-02-25 | 北京航空航天大学 | Pourable vascular tissue engineering reactor having rotating function |
| KR20110073949A (en) * | 2009-12-24 | 2011-06-30 | 연세대학교 산학협력단 | A method for regenerating tubelar-type tissues and organs, and thd bioreactor system |
| CN102872478A (en) * | 2012-10-19 | 2013-01-16 | 陕西正凡科技发展有限公司 | Method for constructing full-biological tissue engineering blood vessel |
| CN106264745A (en) * | 2016-08-03 | 2017-01-04 | 苏州大学附属第二医院 | Tumor-microvessel self assembly generation system |
| CN205874451U (en) * | 2016-08-03 | 2017-01-11 | 苏州大学附属第二医院 | Artificial blood vessel's cultivation and function detecting device |
| CN106591121A (en) * | 2016-11-30 | 2017-04-26 | 广州博敏科技有限公司 | Rotation culture chamber and rotation pulsation culture system |
| US20210222130A1 (en) * | 2018-08-07 | 2021-07-22 | 1Lab Sa | Model for in-vitro simulation of the behaviour of dysfunctional vessels |
| CN210048789U (en) * | 2019-04-01 | 2020-02-11 | 刘亮 | Artificial blood vessel endothelialization culture apparatus |
| CN111603611A (en) * | 2020-03-06 | 2020-09-01 | 广东省人民医院(广东省医学科学院) | Cell-derived matrix tubular scaffold and preparation method thereof |
| CN214263118U (en) * | 2020-10-30 | 2021-09-24 | 湖北华电江陵发电有限公司 | Automatic cleaning device |
Non-Patent Citations (3)
| Title |
|---|
| 宋俊等: "《碳中和与低碳能源》", 30 September 2022, 北京机械工业出版社, pages: 130 - 131 * |
| 张燕搏;姜明;王强;吴清玉;胡平;: "动态反应器构建组织工程化血管的初步实验研究", 中华实用诊断与治疗杂志, vol. 25, no. 11, 10 November 2011 (2011-11-10), pages 1047 - 1049 * |
| 李茂本: "《电力生产知识》", 31 December 1994, 中国电力出版社, pages: 126 - 127 * |
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