CN115845004B - Traditional Chinese medicine microcapsule for treating pig diarrhea and preparation method thereof - Google Patents
Traditional Chinese medicine microcapsule for treating pig diarrhea and preparation method thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine microcapsule for treating pig diarrhea and a preparation method thereof, and relates to the technical field of traditional Chinese veterinary medicines. The traditional Chinese medicine microcapsule is prepared from pulsatilla chinensis, dandelion, dark plum, dried orange peel, ash bark, kudzuvine root, costustoot, common andrographis herb, white hyacinth bean, largehead atractylodes rhizome, poria cocos, coix seed and radish seed by reasonably combining the traditional Chinese medicine microcapsule according to the principle of dialectical treatment of the traditional Chinese medicine, the functions of the medicines are fully exerted, and the medicines coordinate the gain efficacy. The traditional Chinese medicine microcapsule has obvious antibacterial effect on escherichia coli, has an immunopotentiating effect on a parturient sow, effectively improves the morbidity and morbidity degree of piglet diarrhea, has a good treatment effect on bacterial diarrhea and viral diarrhea, effectively reduces diarrhea symptoms of the ill pig, improves feed intake of the ill pig, and has a high cure rate on the ill pig.
Description
Technical Field
The invention relates to the technical field of traditional Chinese veterinary medicines, in particular to a traditional Chinese medicine microcapsule for treating pig diarrhea and a preparation method thereof.
Background
At present, the breeding work is continuously developed, the breeding scale is continuously enlarged, the incidence rate of diarrhea diseases of live pigs is higher, if the live pigs are not subjected to scientific and effective treatment measures during the breeding work, the live pigs are easily affected by diarrhea, the growth and development of the live pigs are restricted, and dead cases can also occur in serious cases. The biological diarrhea is caused by various reasons, wherein bacterial diarrhea diseases and viral diarrhea diseases are mainly classified, antibiotics and antibacterial drugs are mostly adopted to prevent secondary infection in the actual cultivation process, the ideal treatment effect is often not achieved, and a series of problems such as animal drug resistance, animal food quality safety, ecological environment pollution and the like exist at the same time.
The Chinese medicinal preparation is a pure natural medicament, contains a large amount of nutrient substances, has the effects of regulating immunity, inhibiting virus replication, resisting bacteria, resisting inflammation, relieving fever and easing pain, does not generate drug resistance, and is a medicament with high safety and low residue. In recent years, various documents and patents report about the content of the traditional Chinese medicine preparation for preventing and treating pig diarrhea, but the traditional Chinese medicine preparation has the problems of inaccurate curative effect, long treatment time, poor treatment effect and the like; meanwhile, most of traditional Chinese medicine preparations are oral liquid, granules or decoction, pigs are animals with taste and smell far higher than those of other livestock and poultry, and because the traditional Chinese medicine materials have unique taste, the preparation still can keep the bitter taste or the spicy taste of the traditional Chinese medicine materials, and most of administration modes are stomach irrigation or free feeding with mixed materials, the special smell of the traditional Chinese medicine can obviously influence the feeding desire and feeding rate of the pigs, and the pigs cannot take enough feeding amount, so that ideal treatment effect cannot be realized.
Disclosure of Invention
(One) solving the technical problems
Aiming at the defects of the prior art, the invention provides the traditional Chinese medicine microcapsule for treating the pig diarrhea and the preparation method thereof, and the traditional Chinese medicine microcapsule has the effects of resisting bacteria, resisting viruses, enhancing immunity and the like, has higher cure rate on the pig diarrhea, can cover the special taste of the traditional Chinese medicine raw medicine, and does not influence the feeding of pigs.
(II) technical scheme
In order to achieve the above purpose, the invention is realized by the following technical scheme:
In one aspect, the invention provides a traditional Chinese medicine microcapsule for treating pig diarrhea, which comprises 80-120 parts of a traditional Chinese medicine composition, 60-100 parts of an emulsifying agent and 100-200 parts of granulating auxiliary materials.
Further, the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 20-50 parts of Chinese pulsatilla root, 20-50 parts of dandelion, 10-30 parts of dark plum, 10-30 parts of dried orange peel, 15-30 parts of ash bark, 5-20 parts of kudzuvine root, 5-20 parts of costustoot, 10-30 parts of common andrographis herb, 10-30 parts of white hyacinth bean, 15-30 parts of bighead atractylodes rhizome, 5-20 parts of poria cocos, 5-15 parts of coix seed and 5-20 parts of radish seed.
Further, the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 25-40 parts of Chinese pulsatilla root, 25-40 parts of dandelion, 15-25 parts of dark plum, 15-25 parts of dried orange peel, 20-30 parts of ash bark, 5-15 parts of kudzuvine root, 10-20 parts of costustoot, 10-20 parts of common andrographis herb, 15-25 parts of white hyacinth bean, 15-25 parts of bighead atractylodes rhizome, 10-20 parts of poria cocos, 5-10 parts of coix seed and 5-15 parts of radish seed.
Further, the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 35 parts of Chinese pulsatilla root, 30 parts of dandelion, 20 parts of dark plum, 15 parts of dried orange peel, 25 parts of ash bark, 10 parts of kudzuvine root, 15 parts of costustoot, 10 parts of common andrographis herb, 20 parts of white hyacinth bean, 20 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 5 parts of coix seed and 10 parts of radish seed.
Further, the emulsifier is one or more of hydroxymethyl propyl cellulose, polyethylene glycol, mannitol and glucose.
Further, the granulating auxiliary material is one or more of glyceryl monostearate, fat powder, stearic acid, sweet potato starch, cellulose acetate, acacia, microcrystalline cellulose, maltodextrin and sucrose.
Further, the traditional Chinese medicine microcapsule comprises 90-110 parts of traditional Chinese medicine composition, 80-100 parts of emulsifying agent and 160-200 parts of granulating auxiliary materials.
Further, the traditional Chinese medicine microcapsule comprises 100 parts of a traditional Chinese medicine composition, 90 parts of an emulsifying agent and 180 parts of a granulating auxiliary material.
On the other hand, the invention provides a preparation method of a traditional Chinese medicine microcapsule for treating pig diarrhea, which comprises the following steps:
(1) Weighing the following traditional Chinese medicine raw materials in parts by weight: 25-40 parts of pulsatilla chinensis, 25-40 parts of dandelion, 15-25 parts of dark plum, 15-25 parts of dried orange peel, 20-30 parts of ash bark, 5-15 parts of kudzuvine root, 10-20 parts of costustoot, 10-20 parts of common andrographis herb, 15-25 parts of white hyacinth bean, 15-25 parts of bighead atractylodes rhizome, 10-20 parts of poria cocos, 5-10 parts of coix seed and 5-15 parts of radish seed, soaking the weighed traditional Chinese medicine raw materials in boiling water with the weight being 3-5 times of that of the traditional Chinese medicine raw materials for 10-15 minutes, taking out, spreading for cooling for 1 hour, cutting into thick slices with the size of 5-8mm, superfine grinding each traditional Chinese medicine raw material thick slice, sieving with a sieve with 100-200 meshes, and uniformly stirring to obtain a powdery mixed material for standby;
(2) Soaking the above mixed materials in 35 ℃ deionized water for 60 minutes, adding biological enzyme, stirring uniformly, carrying out enzymolysis for 2 hours at 40-50 ℃, then carrying out biological enzyme inactivation treatment at 60-70 ℃, and separating the materials from the liquid to obtain liquid A for later use;
(3) Adding 1L deionized water into the soaked materials for soaking for 1 hour, decocting the soaked traditional Chinese medicines for 2 hours, filtering to obtain filtrate I, decocting the decocted traditional Chinese medicines for 30-40 minutes again, filtering to obtain filtrate II, mixing filtrates I, II to obtain liquid B, and mixing the liquids A, B to obtain liquid C for later use;
(4) Adding the emulsifying agent and the granulating auxiliary materials into a material dissolving tank, heating to 55-120 ℃, and stirring until the emulsifying agent and the granulating auxiliary materials are completely melted;
(5) Introducing the melted emulsifying agent and the granulating auxiliary materials into a material mixing tank, and adding the liquid C into the material mixing tank, and stirring for 10-30 minutes by high-speed shearing and emulsifying to obtain a mixture for later use;
(6) Introducing the mixture obtained in the step (5) into a storage tank, and preserving heat at 55-120 ℃;
(7) And (3) carrying out spray granulation on the mixture in the storage tank through high-efficiency vertical spray drying equipment, cooling the prepared granules through a cooling dehumidifying dryer, and sieving and collecting the granules through a 50-100-mesh oscillating screen to obtain the microcapsule preparation.
The pharmacological actions of the traditional Chinese medicines in the invention are as follows:
pulsatilla root: bitter taste, cold nature, stomach and large intestine meridians; has effects of clearing heat and detoxicating, cooling blood and relieving dysentery, and is commonly used for treating dysentery with heat toxin and bloody stool, pruritus vulvae and leukorrhagia; the pulsatilla chinensis bunge has the chemical components mainly of triterpenoid saponins and triterpenoid acids, and has certain pharmacological effects in the aspects of resisting tumor, resisting bacteria, resisting inflammation, easing pain and the like.
Dandelion: bitter and sweet in taste, cold in nature, enters liver and stomach meridians; has effects of clearing heat and detoxicating, detumescence and resolving hard mass, promoting urination and treating stranguria; can be used for treating furuncle, acute mastitis, scrofula, conjunctival congestion, pharyngalgia, pulmonary abscess, appendicitis, jaundice due to damp-heat, and stranguria due to heat; the medicine contains flavonoid, coumarin, phenolic acid, terpene, sterol, polysaccharide and other effective components, and clinical research shows that the medicine has bacteriostasis, antioxidation, anti-inflammatory, antitumor, gastrointestinal protection and other pharmacological effects.
Dark plum: sour and astringent taste, smooth nature, and enter liver, spleen and lung meridians; has effects of astringing lung, astringing intestine, promoting salivation, and relieving ascariasis; can be used for treating cough due to lung deficiency, chronic diarrhea, chronic dysentery, deficiency heat, and diabetes.
Dried orange peel: pungent and bitter in flavor, warm in nature, enter spleen and lung meridians; has the effects of regulating qi and eliminating dampness; can be used for treating incoordination between spleen and stomach, emesis, cough, fullness and inappetence.
Cortex Fraxini Radicis: bitter and astringent taste, cold nature; the main indications are: clearing heat, eliminating dampness, astringing, stopping diarrhea, stopping leukorrhagia, and improving eyesight; can be used for treating dysentery due to heat toxin, leucorrhea with reddish discharge, conjunctival congestion with swelling and pain, and nebula.
Radix Puerariae: sweet and pungent in flavor, cool in nature, enter lung and stomach meridians; has effects of relieving muscle and fever, promoting eruption, promoting salivation, quenching thirst, and raising yang to arrest diarrhea; is used for treating fever, pain in neck and back, measles, thirst due to fever, and diarrhea due to heat.
Radix aucklandiae: pungent and bitter in taste and warm in nature; spleen, stomach, large intestine, triple energizer and gallbladder meridians; has effects of activating qi-flowing, relieving pain, invigorating spleen, and resolving food stagnation; clinically, the traditional Chinese medicine composition is mainly used for treating gastrointestinal diseases such as spleen and stomach qi stagnation syndrome, diarrhea, tenesmus and the like; modern pharmacological researches have shown that radix aucklandiae has effects of relieving smooth muscle spasm, lowering blood pressure, resisting bacteria, etc., and also has certain activity in anticancer, immunity, anti-inflammatory etc.
Andrographis paniculata: bitter in flavor and cold in nature. It enters heart, lung, large intestine and bladder meridians. Clearing heat and detoxicating, cooling blood and detumescence. Can be used for treating common cold, fever, sore throat, aphtha of the mouth and tongue, cough due to cough, diarrhea, dysentery, pyretic stranguria, carbuncle, swelling, sore and ulcer, and snake and insect bite.
White hyacinth bean: sweet taste and slightly warm nature; has effects of invigorating spleen, eliminating dampness, regulating stomach function, and removing summer-heat; can be used for treating spleen and stomach weakness, anorexia, loose stool, leukorrhagia, summer-heat, damp vomiting, diarrhea, chest distress, and abdominal distention. The stir-baked hyacinth bean has the effects of strengthening spleen and resolving dampness, and is used for treating spleen deficiency diarrhea, leukorrhagia and the like.
White atractylodes rhizome: bitter and sweet in nature, warm in nature, and enters spleen and stomach meridians; has effects of invigorating spleen, invigorating qi, eliminating dampness, promoting diuresis, relieving sweating, and preventing miscarriage; is often used for spleen deficiency, anorexia, diarrhea, loose stool, fetal movement, etc.; the main chemical components of the composition include volatile oil, polysaccharide, internal lipid, etc., and have the functions of resisting tumor, resisting inflammation, regulating digestive system, etc
Poria cocos: sweet and light in taste and neutral in nature; return to heart, lung, spleen and kidney meridians; has effects in promoting diuresis, removing dampness, invigorating spleen, and calming heart; can be used for treating edema, oliguria, phlegm retention, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia; poria cocos is rich in various chemical components, wherein triterpenes and polysaccharide compounds are main active substances of Poria cocos, and modern pharmacological researches show that Poria cocos has pharmacological activities of resisting tumor, regulating immunity, resisting inflammation, resisting oxidation, resisting aging and the like.
Semen Coicis: sweet and bland in taste, cool in nature, and enters spleen, stomach and lung meridians; has effects in promoting diuresis, promoting moisture permeability, invigorating spleen, relieving diarrhea, removing toxic materials, and resolving hard mass; can be used for treating edema, dysuria, spleen deficiency diarrhea, wet arthralgia, contracture, pulmonary abscess, and appendicitis; the Coicis semen contains fatty acid and various compounds such as esters, polysaccharide, flavone, triterpene, alkaloid, sterol, lactam, and starch, and has effects of inhibiting skeletal muscle contraction, relieving fever, relieving pain, resisting inflammation, resisting tumor, and enhancing immunity.
Radish seed: sweet in flavor, neutral in nature, enters lung and stomach meridians; is used for descending qi, relieving asthma, promoting digestion and resolving phlegm. For cough, dyspnea with phlegm, food stagnation, chest distress, abdominal distention, diarrhea and tenesmus.
(III) beneficial effects
The invention provides a traditional Chinese medicine microcapsule for treating pig diarrhea and a preparation method thereof. The traditional Chinese medicine microcapsule is prepared from pulsatilla chinensis, dandelion, dark plum, dried orange peel, ash bark, kudzuvine root, costustoot, common andrographis herb, white hyacinth bean, largehead atractylodes rhizome, poria cocos, coix seed and radish seed by reasonably combining the traditional Chinese medicine microcapsule according to the principle of dialectical treatment of the traditional Chinese medicine, the functions of the medicines are fully exerted, and the medicines coordinate the gain efficacy. The traditional Chinese medicine microcapsule has obvious antibacterial effect on escherichia coli, has an immunopotentiating effect on a parturient sow, effectively improves the morbidity and morbidity degree of piglet diarrhea, has a good treatment effect on bacterial diarrhea and viral diarrhea, effectively reduces diarrhea symptoms of the ill pig, improves feed intake of the ill pig, and has a high cure rate on the ill pig.
Drawings
FIG. 1 shows serum IgG content of sow in the 0d, 15d and 30d blank control groups, chinese medicinal control groups and Chinese medicinal microcapsule groups according to the invention.
Fig. 2 shows the diarrhea rate of piglets in the blank control group, the traditional Chinese medicine control group and the traditional Chinese medicine microcapsule group.
Fig. 3 shows diarrhea index of piglets in a blank control group, a traditional Chinese medicine control group and a traditional Chinese medicine microcapsule group according to the invention.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described in the following in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
A traditional Chinese medicine microcapsule for treating diarrhea of piglets comprises the following components in parts by weight: 100 parts of traditional Chinese medicine composition, 90 parts of emulsifying agent and 180 parts of granulating auxiliary material.
The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 35 parts of Chinese pulsatilla root, 30 parts of dandelion, 20 parts of dark plum, 15 parts of dried orange peel, 25 parts of ash bark, 10 parts of kudzuvine root, 15 parts of costustoot, 10 parts of common andrographis herb, 20 parts of white hyacinth bean, 20 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 5 parts of coix seed and 10 parts of radish seed.
The emulsifier is one or more of hydroxymethyl propyl cellulose, polyethylene glycol, mannitol and glucose.
The granulating auxiliary material is one or more of glyceryl monostearate, fat powder, stearic acid, sweet potato starch, cellulose acetate, acacia, microcrystalline cellulose, maltodextrin and sucrose.
The traditional Chinese medicine microcapsule is prepared according to the following steps:
(1) Weighing the traditional Chinese medicine raw materials according to the weight parts, soaking the weighed traditional Chinese medicine raw materials in boiling water with the weight being 3-5 times that of the traditional Chinese medicine raw materials for 10-15 minutes, taking out, spreading and cooling for 1 hour, cutting into thick slices with the thickness of 5-8mm, superfine grinding the thick slices of the traditional Chinese medicine raw materials, sieving the thick slices with a 100-200-mesh sieve, and uniformly stirring to obtain a powdery mixed material, namely a traditional Chinese medicine composition for later use;
(2) Soaking the Chinese medicinal composition in 35 ℃ deionized water for 60 minutes, adding biological enzyme, stirring uniformly, performing enzymolysis at 40-50 ℃ for 2 hours, performing biological enzyme inactivation treatment at 60-70 ℃, and separating the materials from the liquid to obtain liquid A for later use;
(3) Adding 1L deionized water into the soaked materials for soaking for 1 hour, decocting the soaked traditional Chinese medicines for 2 hours, filtering to obtain filtrate I, decocting the decocted traditional Chinese medicines for 30-40 minutes again, filtering to obtain filtrate II, mixing filtrates I, II to obtain liquid B, and mixing the liquids A, B to obtain liquid C for later use;
(4): adding the emulsifying agent and the granulating auxiliary materials into a material dissolving tank, heating to 55-120 ℃, and stirring until the emulsifying agent and the granulating auxiliary materials are completely melted;
(5): introducing the melted emulsifying agent and the granulating auxiliary materials into a material mixing tank, and adding the liquid C into the material mixing tank, and stirring for 10-30 minutes by high-speed shearing and emulsifying to obtain a mixture for later use;
(6): introducing the mixture obtained in the step (5) into a storage tank, and preserving heat at 55-120 ℃;
(7): and (3) carrying out spray granulation on the mixture in the storage tank through high-efficiency vertical spray drying equipment, cooling the prepared granules through a cooling dehumidifying dryer, and sieving and collecting the granules through a 50-100-mesh oscillating screen to obtain the microcapsule preparation.
Example 2
This embodiment differs from embodiment 1 in that:
the traditional Chinese medicine microcapsule comprises the following components in parts by weight: 115 parts of traditional Chinese medicine composition, 100 parts of emulsifying agent and 160 parts of granulating auxiliary materials.
Example 3
This embodiment differs from embodiment 1 in that:
The traditional Chinese medicine microcapsule comprises the following components in parts by weight: 90 parts of traditional Chinese medicine composition, 80 parts of emulsifying agent and 160 parts of granulating auxiliary material.
Example 4
This embodiment differs from embodiment 1 in that:
The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 20 parts of pulsatilla chinensis, 20 parts of dandelion, 10 parts of dark plum, 10 parts of dried orange peel, 15 parts of ash bark, 5 parts of kudzuvine root, 5 parts of costustoot, 10 parts of common andrographis herb, 10 parts of white hyacinth bean, 15 parts of bighead atractylodes rhizome, 5 parts of poria cocos, 5 parts of coix seed and 5 parts of radish seed.
Example 5
This embodiment differs from embodiment 1 in that:
The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 45 parts of pulsatilla chinensis, 45 parts of dandelion, 10 parts of dark plum, 10 parts of dried orange peel, 15 parts of ash bark, 20 parts of kudzuvine root, 20 parts of costustoot, 25 parts of common andrographis herb, 25 parts of white hyacinth bean, 25 parts of bighead atractylodes rhizome, 20 parts of poria cocos, 15 parts of coix seed and 20 parts of radish seed.
Example 6
This embodiment differs from embodiment 1 in that:
the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 25 parts of pulsatilla chinensis, 25 parts of dandelion, 15 parts of dark plum, 15 parts of dried orange peel, 20 parts of ash bark, 5 parts of kudzuvine root, 10 parts of costustoot, 10 parts of common andrographis herb, 15 parts of white hyacinth bean, 15 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 5 parts of coix seed and 5 parts of radish seed.
Example 7
This embodiment differs from embodiment 1 in that:
The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 40 parts of pulsatilla chinensis, 40 parts of dandelion, 25 parts of dark plum, 25 parts of dried orange peel, 30 parts of ash bark, 15 parts of kudzuvine root, 20 parts of costustoot, 20 parts of common andrographis herb, 25 parts of white hyacinth bean, 25 parts of bighead atractylodes rhizome, 20 parts of poria cocos, 10 parts of coix seed and 15 parts of radish seed.
Example 8
This embodiment differs from embodiment 1 in that:
The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 32 parts of pulsatilla chinensis, 28 parts of dandelion, 17 parts of dark plum, 18 parts of dried orange peel, 22 parts of ash bark, 9 parts of kudzuvine root, 12 parts of costustoot, 14 parts of common andrographis herb, 19 parts of white hyacinth bean, 22 parts of bighead atractylodes rhizome, 14 parts of poria cocos, 7 parts of coix seed and 9 parts of radish seed.
Example 9
This embodiment differs from embodiment 1 in that:
The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 28 parts of Chinese pulsatilla root, 36 parts of dandelion, 14 parts of dark plum, 22 parts of dried orange peel, 23 parts of ash bark, 12 parts of kudzuvine root, 18 parts of costustoot, 14 parts of common andrographis herb, 14 parts of white hyacinth bean, 22 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 8 parts of coix seed and 11 parts of radish seed.
Test example 1
The traditional Chinese medicine composition has reasonable compatibility of medicines, fully plays the roles of the medicines, and co-coordinates the gain efficacy of the medicines, and because of more experimental data, the traditional Chinese medicine composition adjusts the medicines for multiple times and performs experiments, only the antibacterial effect of a plurality of groups of formulas on escherichia coli is listed and compared with the formula of the traditional Chinese medicine composition.
Formula 1: 30 parts of dandelion, 20 parts of dark plum, 15 parts of dried orange peel, 25 parts of ash bark, 10 parts of kudzuvine root, 20 parts of white hyacinth bean, 20 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 5 parts of coix seed and 10 parts of radish seed.
And 2, a formula: 35 parts of pulsatilla chinensis, 20 parts of dark plum, 15 parts of dried orange peel, 25 parts of ash bark, 10 parts of kudzuvine root, 15 parts of costustoot, 10 parts of common andrographis herb, 10 parts of Indian buead, 5 parts of coix seed and 10 parts of radish seed.
And 3, formula 3: 35 parts of pulsatilla chinensis, 30 parts of dandelion, 10 parts of radix puerariae, 15 parts of elecampane, 10 parts of common andrographis herb, 20 parts of white hyacinth bean, 20 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 5 parts of coix seed and 10 parts of radish seed.
And (4) a formula: 35 parts of pulsatilla chinensis, 30 parts of dandelion, 20 parts of dark plum, 15 parts of dried orange peel, 25 parts of ash bark, 15 parts of costustoot, 10 parts of common andrographis herb, 20 parts of white hyacinth bean and 20 parts of bighead atractylodes rhizome.
The formula of the invention comprises the following components: 35 parts of Chinese pulsatilla root, 30 parts of dandelion, 20 parts of dark plum, 15 parts of dried orange peel, 25 parts of ash bark, 10 parts of kudzuvine root, 15 parts of costustoot, 10 parts of common andrographis herb, 20 parts of white hyacinth bean, 20 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 5 parts of coix seed and 10 parts of radish seed.
The formula of the commercially available traditional Chinese medicine products comprises the following components: radix Pulsatillae powder, radix Pulsatillae, coptidis rhizoma, cortex Phellodendri and cortex Fraxini.
The preparation method of the traditional Chinese medicine stock solution comprises the following steps: cleaning the above Chinese medicinal materials, oven drying, pulverizing, and decocting to obtain 1g/mL (1 g per mL of crude drug), sterilizing, and storing in refrigerator at 0-4deg.C.
In vitro bacteriostasis test:
the test indicator bacteria were E.coli isolated from diarrhea piglets. And (3) taking activated escherichia coli bacterial sludge, diluting to the viable count of about 10 8 CFU/mL, sucking 2mL of bacterial suspension, adding the bacterial suspension into 200mL of nutrient agar culture medium with the temperature of 50 ℃ for shaking uniformly, pouring the flat plate, cooling and solidifying, and placing the sterilized oxford cup on the flat plate. 0.1mL of traditional Chinese medicine concentrated solution and western medicines with different formulas are respectively added into oxford cups, and the oxford cups are cultured for 24 hours at 37 ℃ and 5 times of culture are repeated for each group. Observing whether a bacteriostasis ring is generated or not, and measuring the diameter of the bacteriostasis ring by using a vernier caliper. The results are shown in Table 1.
From the results in table 1, it can be seen that the medicines used in each group have a certain antibacterial effect, and the larger the diameter of the antibacterial ring is, the better the antibacterial effect is; compared with a traditional Chinese medicine control group, the diameter of the inhibition zone of the groups 1-4 and the traditional Chinese medicine formula group is obviously higher than that of the traditional Chinese medicine control group (P < 0.05); compared with the traditional Chinese medicine formula, the diameter of the inhibition zone of the formula 1-4 is obviously lower than that of the traditional Chinese medicine formula (P < 0.05), and the difference is obvious (P < 0.05); the results show that the inhibiting effect of the traditional Chinese medicine composition on escherichia coli is obviously better than that of the pulsatilla chinensis powder, and when the traditional Chinese medicines in the traditional Chinese medicine composition are not partially provided, the ideal antibacterial effect cannot be realized.
TABLE 1 in vitro bacteriostatic effects of the inventive Chinese medicinal formulation
| Grouping | Diameter of inhibition zone (mm) |
| Traditional Chinese medicine control group | 6.86±0.35a |
| Formula 1 group | 8.50±0.42b |
| Formula 2 group | 7.46±0.27a |
| 3 Groups of formula | 8.70±0.37b |
| Formula 4 group | 9.58±0.31c |
| The invention relates to a traditional Chinese medicine formula | 14.86±0.24d |
Note that: the same column data shoulder indicates that the difference is significant (P < 0.05) and the same column data shoulder indicates that the difference is not significant (P > 0.05).
Test example 2
1 Materials and methods
1.1 Test animals and groups
15 Healthy sows 1 month before parturition are randomly divided into 3 groups, and 5 sows in each group are respectively a blank control group and a traditional Chinese medicine control group. Wherein the blank group is fed with normal daily feed every day; the traditional Chinese medicine control group is prepared by adding 50g/kg of radix anemones in daily ration; the traditional Chinese medicine microcapsule group is prepared by adding 50g/Kg of the traditional Chinese medicine microcapsule prepared in the example 1 into daily ration; during the test period, the daily management of the sow and the piglet is carried out according to the daily feeding management. Blood was collected from the fasting ear vein of sows at day 0, day 15 and day 30, respectively, after the start of the test, and stored at-20 ℃ for humoral immunity index determination.
1.2 Method
1.2.1 Humoral immunoassay methods
Specific IgG content was determined according to the pig IgG ELASA assay kit instructions.
1.2.2 Piglet diarrhea evaluation method
Postnatal 14d is the test period of diarrhea in piglets. The diarrhea rate of piglets is the percentage of the number of diarrhea piglets to the total number of piglets to be tested; the diarrhea index is the ratio of the piglet fecal score to the total number of piglets tested. Grading diarrhea in piglets, referring to method Yan Cheng et al (2012), as apparent bar or granular stool, rated 0 points; soft manure, shaping, and evaluating 1 minute; thick, non-formed, no separation of fecal sewage, rated as 2 minutes; no formation and manure-water separation were carried out, and the score was 3.
1.3 Data processing
Analysis was performed using Prism 8.0.2 statistical software.
2 Results
2.1 Effect of the Chinese medicinal microcapsule of the invention on the serum IgG content of sow
As can be seen from fig. 1, on day 15 of administration, serum IgG content of the traditional Chinese medicine control group and the traditional Chinese medicine microcapsule group of the invention is significantly increased compared with that of the control group, and the difference is significant (P < 0.05); on the 30 th day of administration, the serum IgG content of the traditional Chinese medicine control group and the traditional Chinese medicine microcapsule group is continuously increased, the difference is obvious (P is less than 0.05) compared with the control group, the serum IgG content of the traditional Chinese medicine microcapsule group is obviously increased compared with the traditional Chinese medicine control group, and the difference is obvious (P is less than 0.05).
2.2 Effect of the Chinese medicinal microcapsule of the present invention on diarrhea of piglets
As can be seen from fig. 2, the diarrhea rate of the piglets of the traditional Chinese medicine control group is 10.47%, and the diarrhea rate of the piglets is significantly different (P < 0.05) compared with the diarrhea rate of the piglets of the blank control group (14.36); the diarrhea rate of the piglets in the traditional Chinese medicine microcapsule group is 6.49%, and compared with a blank control group, the diarrhea rate of the piglets in the traditional Chinese medicine microcapsule group is obviously different (P is less than 0.05); the diarrhea rate of the piglets of the traditional Chinese medicine microcapsule group is obviously lower than that of the traditional Chinese medicine control group, and the difference is obvious (P is less than 0.05).
As can be seen from fig. 3, the diarrhea index of the piglets of the traditional Chinese medicine control group is 0.42, and the diarrhea index of the piglets of the traditional Chinese medicine control group is significantly different (P < 0.05) from that of the piglets of the blank control group (0.67); the abdomen index of the piglets in the traditional Chinese medicine microcapsule group is 0.11, and compared with the blank control group, the difference is obvious (P is less than 0.05); the diarrhea index of the piglets of the traditional Chinese medicine microcapsule group is obviously lower than that of the traditional Chinese medicine control group, and the difference is obvious (P is less than 0.05).
Conclusion 3
The test results show that the IgG content in serum can be increased by feeding the Chinese medicine or the Chinese medicine microcapsule to the parturient sow, and the increase of the Chinese medicine microcapsule is more obvious, which proves that the Chinese medicine microcapsule is more effective in improving the immunity of the sow than the classical Chinese medicine.
The diarrhea rate and diarrhea index can be effectively reduced by adding the traditional Chinese medicine or the traditional Chinese medicine microcapsule in the ration of the parturient sow, and the traditional Chinese medicine microcapsule has more obvious effect. The reasonable addition of the traditional Chinese medicine microcapsule in the daily ration of the sow can effectively improve the incidence and incidence degree of diarrhea of piglets.
Test example 3
The weight of a certain pig farm and the total of 90 yellow and white diarrhea piglets with similar ages are selected, and divided into 3 groups, namely a western medicine control group, a traditional Chinese medicine control group and a traditional Chinese medicine microcapsule group, wherein 30 groups are divided into 3 parallel groups, 10 groups are respectively divided into 10 groups, and the treatment method of each group is shown in Table 2. The piglets can drink water and eat artificial milk freely during the test.
Curative effect judgment criteria:
and (3) curing: the yellow and white scour of piglets disappears, the feces are formed, the feeding amount is normal, and the mental state is good;
the method is effective: the times of yellow and white diarrhea of piglets are reduced, the fecal character is recovered, and the feeding amount is increased;
Invalidation: the feed intake and diarrhea symptoms are not improved, and the times of yellow and white diarrhea of piglets are unchanged and even die before treatment.
Table 2 test groups and treatment regimens
Table 3 shows the therapeutic effect of each group of piglets during the trial. As can be seen from the results in Table 3, the effective number and cure number of the Chinese medicinal microcapsule group are obviously higher than those of the western medicine control group and the Chinese medicinal control group, and the difference is obvious (P is less than 0.05); the invalid number and death number of the traditional Chinese medicine microcapsule group are obviously lower than those of a western medicine control group and a traditional Chinese medicine control group, and the differences are obvious (P is less than 0.05). The test results show that compared with western medicines and other traditional Chinese medicines, the traditional Chinese medicine microcapsule disclosed by the invention has a better treatment effect on diarrhea piglets due to yellow and white dysentery, can effectively improve diarrhea symptoms of piglets, reduce death occurrence and improve breeding benefits.
Table 3 effects of each group on treatment of diarrhea in yellow and white diarrhea piglets
| Group of | Effective (head) | Cure (head) | Invalidation (head) | Death (head) |
| Western medicine control group | 6.33±0.58a | 4.33±0.58a | 3.67±0.58a | 0.67±0.58a |
| Traditional Chinese medicine control group | 5.00±1.00a | 4.00±1.00a | 5.00±1.00a | 1.33±0.58a |
| The invention relates to a traditional Chinese medicine microcapsule group | 9.33±0.58b | 8.33±0.58b | 0.67±0.58b | 0b |
Test example 4
The fattening pig group in a certain scale of the farm in 10 months 2021 has severe diarrhea, a large number of fattening pigs die in a short time, and the pig epidemic diarrhea is detected and diagnosed by combining clinical symptoms, pathological changes and detection. The total number of fattening pigs in the farm is 1000, the number of the pigs diagnosed with porcine epidemic diarrhea is 674, the number of the pigs with death disease is 135, the number of the pigs without obvious symptoms is 326, the pigs with confirmed disease and the healthy pigs without symptoms are separately bred, the pigs with confirmed disease and the healthy pigs without symptoms are respectively treated and prevented, the traditional Chinese medicine microcapsule prepared in the example 1 is administered to all pigs in each group, and the traditional Chinese medicine microcapsule is administered by 0.25g/kg mixed feed for 3 times per day. 3 days after administration, the sick pig group stops dying; after 7 days of administration, the diarrhea symptoms of the sick pig groups are obviously improved, the ingestion is increased, and the mental state is good; after administration for 10 days, the diarrhea symptoms of the sick pig group are almost eliminated, the feed intake is recovered to be normal, and the mental state is normal; the overall cure rate of the sick pig group reaches 96 percent. The prophylaxis group did not develop the onset symptoms. The results show that the traditional Chinese medicine microcapsule has a good treatment effect on viral pig diarrhea, has a high cure rate, and has an obvious prevention effect.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. The traditional Chinese medicine microcapsule for treating pig diarrhea is characterized by comprising 80-120 parts of traditional Chinese medicine composition, 60-100 parts of emulsifying agent and 100-200 parts of granulating auxiliary materials; the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 20-50 parts of Chinese pulsatilla root, 20-50 parts of dandelion, 10-30 parts of dark plum, 10-30 parts of dried orange peel, 15-30 parts of ash bark, 5-20 parts of kudzuvine root, 5-20 parts of costustoot, 10-30 parts of common andrographis herb, 10-30 parts of white hyacinth bean, 15-30 parts of bighead atractylodes rhizome, 5-20 parts of poria cocos, 5-15 parts of coix seed and 5-20 parts of radish seed.
2. The traditional Chinese medicine microcapsule for treating porcine diarrhea according to claim 1, wherein the traditional Chinese medicine composition is composed of the following traditional Chinese medicine raw materials in parts by weight: 25-40 parts of Chinese pulsatilla root, 25-40 parts of dandelion, 15-25 parts of dark plum, 15-25 parts of dried orange peel, 20-30 parts of ash bark, 5-15 parts of kudzuvine root, 10-20 parts of costustoot, 10-20 parts of common andrographis herb, 15-25 parts of white hyacinth bean, 15-25 parts of bighead atractylodes rhizome, 10-20 parts of poria cocos, 5-10 parts of coix seed and 5-15 parts of radish seed.
3. The traditional Chinese medicine microcapsule for treating porcine diarrhea according to claim 1, wherein the traditional Chinese medicine composition is composed of the following traditional Chinese medicine raw materials in parts by weight: 35 parts of Chinese pulsatilla root, 30 parts of dandelion, 20 parts of dark plum, 15 parts of dried orange peel, 25 parts of ash bark, 10 parts of kudzuvine root, 15 parts of costustoot, 10 parts of common andrographis herb, 20 parts of white hyacinth bean, 20 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 5 parts of coix seed and 10 parts of radish seed.
4. The traditional Chinese medicine microcapsule for treating porcine diarrhea according to claim 1, wherein the emulsifier is one or more of hydroxymethyl propyl cellulose, polyethylene glycol, mannitol and glucose.
5. The traditional Chinese medicine microcapsule for treating porcine diarrhea according to claim 1, wherein the granulating auxiliary material is one or more of glyceryl monostearate, fat powder, stearic acid, sweet potato starch, cellulose acetate, acacia, microcrystalline cellulose, maltodextrin and sucrose.
6. The traditional Chinese medicine microcapsule for treating porcine diarrhea according to claim 1, wherein the traditional Chinese medicine microcapsule comprises 90-110 parts of a traditional Chinese medicine composition, 80-100 parts of an emulsifying agent and 160-200 parts of granulating auxiliary materials.
7. The traditional Chinese medicine microcapsule for treating porcine diarrhea according to claim 1, wherein the traditional Chinese medicine microcapsule comprises 100 parts of a traditional Chinese medicine composition, 90 parts of an emulsifying agent and 180 parts of a granulating auxiliary material.
8. The method for preparing the traditional Chinese medicine microcapsule for treating porcine diarrhea according to claim 1, wherein the traditional Chinese medicine microcapsule is prepared according to the following steps:
(1) Weighing the following traditional Chinese medicine raw materials in parts by weight: 25-40 parts of pulsatilla chinensis, 25-40 parts of dandelion, 15-25 parts of dark plum, 15-25 parts of dried orange peel, 20-30 parts of ash bark, 5-15 parts of kudzuvine root, 10-20 parts of costustoot, 10-20 parts of common andrographis herb, 15-25 parts of white hyacinth bean, 15-25 parts of bighead atractylodes rhizome, 10-20 parts of poria cocos, 5-10 parts of coix seed and 5-15 parts of radish seed, soaking the weighed traditional Chinese medicine raw materials in boiling water with the weight being 3-5 times of that of the traditional Chinese medicine raw materials for 10-15 minutes, taking out, spreading for cooling for 1 hour, cutting into thick slices with the size of 5-8mm, superfine grinding each traditional Chinese medicine raw material thick slice, sieving with a sieve with 100-200 meshes, and uniformly stirring to obtain a powdery mixed material for standby;
(2) Soaking the above mixed materials in 35 ℃ deionized water for 60 minutes, adding biological enzyme, stirring uniformly, carrying out enzymolysis for 2 hours at 40-50 ℃, then carrying out biological enzyme inactivation treatment at 60-70 ℃, and separating the materials from the liquid to obtain liquid A for later use;
(3) Adding 1L deionized water into the soaked materials for soaking for 1 hour, decocting the soaked traditional Chinese medicines for 2 hours, filtering to obtain filtrate I, decocting the decocted traditional Chinese medicines for 30-40 minutes again, filtering to obtain filtrate II, mixing filtrates I, II to obtain liquid B, and mixing the liquids A, B to obtain liquid C for later use;
(4) Adding the emulsifying agent and the granulating auxiliary materials into a material dissolving tank, heating to 55-120 ℃, and stirring until the emulsifying agent and the granulating auxiliary materials are completely melted;
(5) Introducing the melted emulsifying agent and the granulating auxiliary materials into a material mixing tank, and adding the liquid C into the material mixing tank, and stirring for 10-30 minutes by high-speed shearing and emulsifying to obtain a mixture for later use;
(6) Introducing the mixture obtained in the step (5) into a storage tank, and preserving heat at 55-120 ℃;
(7) And (3) carrying out spray granulation on the mixture in the storage tank through high-efficiency vertical spray drying equipment, cooling the prepared granules through a cooling dehumidifying dryer, and sieving and collecting the granules through a 50-100-mesh oscillating screen to obtain the microcapsule preparation.
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| CN103006765A (en) * | 2012-12-28 | 2013-04-03 | 周春祥 | Pig disease preventing and treating traditional Chinese medicine (TCM) composition for improving immunity |
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