CN114563380A - Correlation receiving type fluorescence detection structure - Google Patents
Correlation receiving type fluorescence detection structure Download PDFInfo
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- CN114563380A CN114563380A CN202111366732.9A CN202111366732A CN114563380A CN 114563380 A CN114563380 A CN 114563380A CN 202111366732 A CN202111366732 A CN 202111366732A CN 114563380 A CN114563380 A CN 114563380A
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 18
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- 230000003287 optical effect Effects 0.000 claims abstract description 13
- 238000001816 cooling Methods 0.000 claims abstract description 8
- 239000004065 semiconductor Substances 0.000 claims abstract description 8
- 230000017525 heat dissipation Effects 0.000 claims description 27
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- 229910002804 graphite Inorganic materials 0.000 claims description 7
- 239000010439 graphite Substances 0.000 claims description 7
- 238000013461 design Methods 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 abstract description 6
- 230000005540 biological transmission Effects 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 6
- 238000004451 qualitative analysis Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011900 installation process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Molecular Biology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a correlation receiving type fluorescence detection structure which comprises a radiating fin, a heating circuit board, a reagent tube, a lamp source, a receiver, a receiving optical filter, an emission optical filter, a semiconductor cooling fin and the like, wherein the lamp source device comprises a lamp source plate and a lamp source, and the receiver comprises a receiver plate, a receiver plug and a photoelectric receiver. The invention belongs to the technical field of detection, and particularly relates to a fluorescence detection device for a PCR instrument. The emission and the receiving of the lamp source in the structure adopt a vertical light path design, so that the signal loss caused by the refraction and the transmission of light is avoided; meanwhile, the whole set of optical path structure and the reagent tube are integrally designed, so that the structure is compact, the sealing is good, and the interference of an external light source is effectively avoided; in addition, compared with the prior art, the structure improves the performance and reduces the manufacturing cost.
Description
Technical Field
The invention belongs to the technical field of detection, and particularly relates to a correlation receiving type fluorescence detection device.
Background
The fluorescence detection device of the PCR instrument on the market at present mostly adopts a multi-channel integrated design, an emission end, a scanning end and a receiving end are integrated together, multi-channel detection is completed through the movement of the scanning end and is used for quantitative analysis and detection, but for the detection only needing qualitative analysis, the device is inevitably wasted; meanwhile, in the devices commonly adopted in the market, the light path design mostly adopts a form of a refractor, so that the requirements on the installation process are high, and the vertical incidence of light is inconvenient.
Disclosure of Invention
Aiming at the defects of the prior art, the invention discloses a correlation receiving type fluorescence detection device, which mainly aims at the detection occasions needing qualitative analysis. Meanwhile, the light source emission and the fluorescence reception adopt a vertical light path design, the connection of a traditional optical fiber bundle is avoided, the light signal is not required to be transmitted through the refraction of light, the light loss is reduced, and the fluorescence signal receiving efficiency and accuracy are improved.
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a correlation reception formula fluorescence detection structure, its characterized in that includes container board, lamp source device, receiver, receiving filter, emission light filter and reagent pipe, be provided with a plurality of container seats that are used for the installation on the container board the reagent pipe, one side of container seat has set gradually emission light filter, lamp source device, the opposite side of container seat has set gradually receiving filter, receiver, horizontal light trap has been seted up to the lower part of container seat, the light that the lamp source device transmitted arrives receiving filter through emission light filter, horizontal light trap, reagent pipe.
Preferably, the heat conduction graphite flake of container board below, heat conduction graphite flake below is provided with the semiconductor cooling fin, semiconductor cooling fin below is provided with the heating circuit board, heating circuit board below is provided with heat abstractor.
Preferably, the heat dissipation device comprises a heat dissipation support, a heat dissipation fin, a heat dissipation connecting plate and a heat sensor, the heat dissipation support is fixed on two sides of the heat dissipation fin through bolts, the heat dissipation connecting plate is installed above the heat dissipation fin, the heat sensor is installed inside the heat dissipation fin and extends out of the heat dissipation support, and the heat dissipation fin is arranged below the heating circuit board.
Preferably, the lamp source device comprises a lamp source plate and a lamp source, the lamp source plate is installed on one side of the container base, and the lamp source is installed on the lamp source plate at a position corresponding to the transverse light through hole.
Preferably, the receiver comprises a receiver plate, a receiver plug and a photoelectric receiver, the receiver plate is mounted on the other side of the container seat, the photoelectric receiver is mounted on one side, close to the receiving optical filter, of the receiver plate, and the receiver plug is mounted in the middle of one side, far away from the receiving optical filter, of the receiver plate.
Preferably, the container plate top install fixed roof and PCR container panel, the draw-in groove has evenly been seted up to fixed roof lower floor, lamp source device, receiver, emission light filter, receiving light filter top are passed through fixed roof draw-in groove is fixed, fixed roof inlays and locates in the PCR container panel, the PCR container panel pass through the bolt with the fin is fixed, heating circuit board passes through the bolt fastening on the PCR container panel.
Preferably, the fixed top plate is made of PPO materials.
Has the advantages that:
the invention provides a correlation receiving type fluorescence detection structure, which has the following beneficial effects that the light is transmitted and received by adopting a vertical light path design, so that the signal loss caused by the refraction and transmission of the light is avoided. The whole set of light path structure and the reagent tube are integrally designed, so that the structure is compact, the sealing is good, and the interference of an external light source is effectively avoided. Compared with the prior art, the device has the advantages of reduced cost, simplified structure and convenient assembly and maintenance.
Drawings
FIG. 1 is a schematic diagram of a correlation receiving fluorescence detection structure according to the present invention;
FIG. 2 is an exploded view of a correlation receiving fluorescence detection structure according to the present invention;
FIG. 3 is a schematic diagram of the photodetection of the correlation receiving type fluorescence detection structure according to the present invention.
Reference numerals: 1-a heat dissipation bracket; 2-a heat sink; 3-a heat dissipation connecting plate; 4-heating the circuit board; 5-PCR vessel panel; 6-fixing the top plate; 7-reagent tube; 8-a container plate; 9-lamp source: 9-1 lamp source plate, 9-2 lamp source; 10-receiver: 10-1 receiver board, 10-2 receiver plug, 10-3 photoelectric receiver; 11-a thermal sensor; 12-a receiving filter; 13-an emission filter; 14-a thermally conductive graphite sheet; 15-semiconductor cooling fins; 16-positioning pin.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: the utility model provides a correlation reception formula fluorescence detection structure, including container board 8, lamp source device 9, the receiver 10, receive optical filter 12, emission light filter 13 and reagent pipe 7, be provided with a plurality of container seats that are used for installing reagent pipe 7 on the container board 8, one side of container seat has set gradually emission light filter 1, lamp source device 9, the opposite side of container seat has set gradually reception light filter 12, receiver 10, horizontal light trap has been seted up to the lower part of container seat, the light that lamp source device 9 transmitted passes through emission light filter 13, horizontal light trap, reagent pipe 7 reachs reception light filter 12.
In one embodiment of the present invention, when the light source 9 is turned on, a single color light is emitted and directly irradiated on the emission filter 13, only the light with the wavelength required for exciting the fluorescence is left through the filter, and then the exciting light is directly irradiated on the reagent sample in the reagent tube 7 through the lateral light hole on one side of the container plate 8. The reagent sample in the reagent tube 7 contains fluorescent substance, when the emitted light contacts with the fluorescent substance, the fluorescent substance can excite the fluorescent light, the fluorescent light can directly pass through the transverse light hole on the other side of the container plate 8 and irradiate on the receiving filter 12, the unnecessary light is filtered out, the light with the required wavelength is ensured, then the light irradiates on the photoelectric receiver 10-3, the light signal is received, and the fluorescence detection is carried out. The light is transmitted and received by adopting a vertical light path design, so that the signal loss caused by the refraction and transmission of the light is avoided. The whole set of light path structure and the reagent tube are integrally designed, so that the structure is compact, the sealing is good, and the interference of an external light source is effectively avoided. Compared with the prior art, the device has the advantages of reduced cost, simplified structure and convenient assembly and maintenance.
In an embodiment of the present invention, the container further includes a heat conducting graphite sheet 14 disposed below the container plate 8, a semiconductor cooling sheet 15 is disposed below the heat conducting graphite sheet 14, a heating circuit board 4 is disposed below the semiconductor cooling sheet 15, and a heat dissipation device is disposed below the heating circuit board 4.
In an embodiment of the invention, the heat dissipation device comprises a heat dissipation support 1, a heat dissipation fin 2, a heat dissipation connecting plate 3 and a heat sensor 11, wherein the heat dissipation support 1 and the heat dissipation fin 2 are positioned through a positioning pin 16 and fixed on two sides of the heat dissipation fin 2 through bolts, the heat dissipation connecting plate 3 is installed above the heat dissipation fin 2, the heat sensor 11 is installed inside the heat dissipation fin 2 and extends out of the heat dissipation support 1, and the heat dissipation fin 2 for monitoring the temperature of the whole system is arranged below a heating circuit board 4.
In an embodiment of the invention, the light source device 9 comprises a light source plate 9-1 and a light source 9-2, the light source plate 9-1 is installed on one side of the container plate seat, the light source 9-2 is installed on the light source plate 9 at a position corresponding to the transverse light through hole, and the light source 9 can be a single light source or a double light source, can emit light with a specific wavelength, and performs qualitative analysis, namely single-channel or double-channel qualitative analysis.
In an embodiment of the invention, the receiver 10 comprises a receiver plate 10-1, a receiver plug 10-2 and a photoelectric receiver 10-3, the receiver plate 10-1 is installed on the other side of the container seat, the photoelectric receiver 10-3 is installed on one side of the receiver plate close to the receiving filter, the receiver plug 10-2 and the photoelectric receiver 10-3 are installed on the middle position of one side of the receiver plate 10-1 far away from the receiving filter, and fluorescent signals generated by fluorescent substances excited by a lamp source 9-2 on the lamp source plate 9-1 can be received, photoelectric conversion is carried out on the receiver plate 10-1, and the fluorescent signals are transmitted to a subsequent circuit through the receiver plug 10-2.
In an embodiment of the invention, a fixed top plate 6 and a PCR container panel 5 are arranged above a container plate 8, clamping grooves are uniformly formed in the lower bottom surface of the fixed top plate 6, the upper parts of a light source device 9, a receiver 10, an emission light filter 13 and a receiving light filter 12 are fixed through the clamping grooves of the fixed top plate 6, an optical path is kept stable through the upper clamping groove and the lower clamping groove, a trapezoidal opening is formed in the PCR container panel 5, and the fixed top plate 6 is embedded in the PCR container panel 5 through the trapezoidal opening.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation. The phrase "comprising a defined element does not exclude the presence of other identical elements in the process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The correlation receiving type fluorescence detection structure is characterized by comprising a container plate, a lamp source device, a receiver, a receiving optical filter, an emission optical filter and a reagent tube, wherein a plurality of container seats for mounting the reagent tube are arranged on the container plate, the emission optical filter and the lamp source device are sequentially arranged on one side of each container seat, the receiving optical filter and the receiver are sequentially arranged on the other side of each container seat, a transverse light hole is formed in the lower portion of each container seat, and light emitted by the lamp source device reaches the receiving optical filter through the emission optical filter, the transverse light hole and the reagent tube.
2. The correlation-emission-reception-type fluorescence detection structure according to claim 1, further comprising a heat-conducting graphite sheet disposed below the container plate, wherein a semiconductor cooling sheet is disposed below the heat-conducting graphite sheet, a heating circuit board is disposed below the semiconductor cooling sheet, and a heat dissipation device is disposed below the heating circuit board.
3. The structure of claim 2, wherein the heat sink comprises a heat sink bracket, a heat sink, a heat-dissipating connecting plate, and a heat sensor, the heat sink bracket is fixed on two sides of the heat sink by bolts, the heat-dissipating connecting plate is mounted above the heat sink, the heat sensor is mounted inside the heat sink and extends out of the heat sink bracket, and the heat sink is disposed below the heating circuit board.
4. The structure of claim 1, wherein the light source device comprises a light source board and a light source, the light source board is installed on one side of the container holder, and the light source is installed on the light source board at a position corresponding to the transverse light-passing hole.
5. A correlation receiving type fluorescence detection structure as claimed in claim 1, wherein said receiver comprises a receiver plate, a receiver plug and a photoelectric receiver, said receiver plate is installed on the other side of said container holder, said photoelectric receiver is installed on one side of said receiver plate close to the receiving filter, and said receiver plug is installed on the middle position of one side of said receiver plate far from the receiving filter.
6. The correlation receiving type fluorescence detection structure of claim 1, wherein a fixing top plate and a PCR container panel are installed above the container plate, clamping grooves are uniformly formed in the lower bottom surface of the fixing top plate, the upper portions of the lamp source device, the receiver, the emission filter and the receiving filter are fixed through the clamping grooves of the fixing top plate, the fixing top plate is embedded in the PCR container panel, the PCR container panel is fixed with the radiating fins through bolts, and the heating circuit board is fixed on the PCR container panel through bolts.
7. The structure as claimed in claim 6, wherein the top fixing plate is made of PPO.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111366732.9A CN114563380A (en) | 2021-11-18 | 2021-11-18 | Correlation receiving type fluorescence detection structure |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111366732.9A CN114563380A (en) | 2021-11-18 | 2021-11-18 | Correlation receiving type fluorescence detection structure |
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| CN114563380A true CN114563380A (en) | 2022-05-31 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2775652Y (en) * | 2004-09-14 | 2006-04-26 | 聂智明 | Multiple PCR quantitative detector |
| US20060152727A1 (en) * | 2005-01-07 | 2006-07-13 | Bickmore William D Jr | Fluorescence detection system |
| CN201438176U (en) * | 2009-07-13 | 2010-04-14 | 天津市兰标电子科技发展有限公司 | Sample fluorescence detection device |
| CN210237595U (en) * | 2019-11-18 | 2020-04-03 | 苏州丹宝医疗科技有限公司 | PCR fluorescence detection system |
| CN112362620A (en) * | 2020-10-16 | 2021-02-12 | 石家庄禾柏生物技术股份有限公司 | Multi-methodology combined optical path |
| CN113564039A (en) * | 2021-07-21 | 2021-10-29 | 厦门海荭兴仪器股份有限公司 | Portable real-time fluorescence quantitative PCR instrument |
-
2021
- 2021-11-18 CN CN202111366732.9A patent/CN114563380A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2775652Y (en) * | 2004-09-14 | 2006-04-26 | 聂智明 | Multiple PCR quantitative detector |
| US20060152727A1 (en) * | 2005-01-07 | 2006-07-13 | Bickmore William D Jr | Fluorescence detection system |
| CN201438176U (en) * | 2009-07-13 | 2010-04-14 | 天津市兰标电子科技发展有限公司 | Sample fluorescence detection device |
| CN210237595U (en) * | 2019-11-18 | 2020-04-03 | 苏州丹宝医疗科技有限公司 | PCR fluorescence detection system |
| CN112362620A (en) * | 2020-10-16 | 2021-02-12 | 石家庄禾柏生物技术股份有限公司 | Multi-methodology combined optical path |
| CN113564039A (en) * | 2021-07-21 | 2021-10-29 | 厦门海荭兴仪器股份有限公司 | Portable real-time fluorescence quantitative PCR instrument |
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