CN103800559A - External traditional Chinese medicinal composition with soothing and whitening effects as well as preparation and preparation method thereof - Google Patents
External traditional Chinese medicinal composition with soothing and whitening effects as well as preparation and preparation method thereof Download PDFInfo
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- CN103800559A CN103800559A CN201410018343.0A CN201410018343A CN103800559A CN 103800559 A CN103800559 A CN 103800559A CN 201410018343 A CN201410018343 A CN 201410018343A CN 103800559 A CN103800559 A CN 103800559A
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Abstract
The invention provides an external traditional Chinese medicinal composition with soothing and whitening effects as well as a preparation and a preparation method thereof. The external traditional Chinese medicinal composition is prepared by taking traditional Chinese medical theory as basis, skin health as a principle and traditional Chinese medicinal extracts as main effective additives, and is assisted by common matrix components in cosmetics. The external traditional Chinese medicinal composition and the preparation thereof has the effects of soothing and whitening, is natural, green, healthy and safe in traditional Chinese medicinal extracts, and can be widely applied to cosmetics with soothing effect.
Description
Technical field
The present invention relates to a kind of external medicine composition, specifically a kind of Chinese medicine preparation that there is the external medicine composition of the white-skinned face function of releiving and prepared by this skin care compositions.
Background technology
Skin allergy is a very common physiological reaction.Most of people can have the experience of skin allergy.Along with the development of modern society, the continuous increase that cosmetics, chemicals use, the original sensitizers such as increasing environmental pollution and pollen, some food all make the probability of skin allergy constantly increase.Because making the crowd of sensitive skin, a variety of causes also constantly increases.The research of the sensitivity skin Dermatosis incidence of various countries is shown, sensitive skin has higher incidence rate.
It is reported, Europe, the women of the U.S. and Japan approximately 40%～50% is sensitivity skin.Britain finds in to the research of 2058 study subjects (comprising masculinity and femininity), and approximately 51.4% women and 38.2% male exist the problem of skin sensitivity.Joudain R etc. carries out telephone poll to not agnate 800 American Women's, and in surveyee, 52% people shows that oneself is for sensitive skin.France carries out telephone poll to more than 15 years old crowd, and 52% surveyee is sensitive skin, and wherein great majority are women, and the ratio of masculinity and femininity sensitive subjects is respectively 44% and 59%.And in China, a research of L'Oreal shows, approximately 36.1% women is sensitive skin.
On current Cosmetic Market, except indivedual color make-up brands, all skin protection brands are all released the product for responsive skin, and quantity and type all increasing, and show that responsive skin market is just being subject to the generally attention of manufacturer and its market prospect is wide.
So in sum, developing a cosmetics with the outstanding effect of releiving is to have real meaning and wide market prospect.
Summary of the invention
Primary and foremost purpose of the present invention is to propose a kind of Chinese medicine composition with the white-skinned face function of releiving.
A further object of the present invention is to propose a kind of preparation method of above-mentioned Chinese medicine composition.
Invention thinking of the present invention is:
The present invention utilizes advanced biotechnology, in conjunction with the traditional theory of Chinese medical science of China, Herba Hedyotidis Diffusae, Cortex Lycii, Radix et Caulis Opuntiae Dillenii, Radix Sophorae Flavescentis, Folium Ginkgo, Radix Glycyrrhizae and Radix Et Rhizoma Fagopyri Tatarici are effectively combined, form compound recipe, and set it as active component, be prepared into skin care formulation, its tool white-skinned face function of releiving, thus can effectively prevent and the dissimilar allergic phenomena of releiving.
Herba Hedyotidis Diffusae: patent medicine bitter in the mouth, light, cold in nature.Main effect is heat-clearing and toxic substances removing, medicinal powder for relieving pain knot, promoting urination to remove dampness.Outstanding kind treatment all kinds inflammation.
Cortex Lycii: cold in nature, sweet in the mouth.Function cures mainly: removing heat from blood is except steaming, lung heat clearing pathogenic fire reducing.For deficiency of YIN hectic fever, hectic fever due to YIN-deficiency night sweat, cough due to lung-heat, spitting of blood, epistaxis.
Radix et Caulis Opuntiae Dillenii: cold in nature, bitter in the mouth, puckery.Enter the heart, lung, stomach three warps.There is heat-clearing and toxic substances removing, relaxing muscles and tendons and activating QI and blood in the collateral, dissipating blood stasis for subsidence of swelling, intestinal detoxication, cooling blood and relieving pain, intestine moistening hemostasis, benefiting stomach and stopping pain, effect of antitussive.
Radix Sophorae Flavescentis: bitter in the mouth; Cold in nature.Return liver; Kidney; Large intestine; Small intestine meridian; Bladder; Heart channel; Urinary bladder channel.There is heat clearing and damp drying, parasite killing, diuresis effect.
Folium Ginkgo: nature and flavor are sweet, bitter, puckery is flat.GUIXIN, lung meridian.Have and astringe the lung, relieving asthma, blood circulation promoting and blood stasis dispelling, effect of pain relieving.
Radix Glycyrrhizae: nature and flavor are sweet, flat.Enter spleen, stomach, lung meridian.Function cures mainly heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity abdomen etc.
Radix Et Rhizoma Fagopyri Tatarici: nature and flavor hardship, flat, cold, has the effect of physical strength profiting, continuous spirit, sharp knowledge, the wide intestinal stomach invigorating of sending down the abnormal ascending QI.
For realizing object of the present invention, the present invention adopts following technical scheme:
There is an external medicine composition for the white-skinned face function of releiving, made by the crude drug of following weight parts proportioning:
Herba Hedyotidis Diffusae 10～30, Cortex Lycii 10～30, Radix et Caulis Opuntiae Dillenii 10～20, Radix Sophorae Flavescentis 10～20, Folium Ginkgo 10～20, Radix Glycyrrhizae 10～20, Radix Et Rhizoma Fagopyri Tatarici 10～20.
In described external medicine composition, the better weight portion proportioning of each crude drug is:
Herba Hedyotidis Diffusae 20～30, Cortex Lycii 20～30, Radix et Caulis Opuntiae Dillenii 15～20, Radix Sophorae Flavescentis 15～20, Folium Ginkgo 10～15, Radix Glycyrrhizae 10～15, Radix Et Rhizoma Fagopyri Tatarici 10～15.
A preparation method for above-mentioned external medicine composition, comprises the steps:
(1) according to above-mentioned weight portion proportioning weighting raw materials;
(2) percent by volume is 75%～80% ethanol extraction twice, extracts for the first time crude drug and ethanol mass volume ratio g/ml is 1:10～1:20, and 60 ℃～70 ℃ heated and stirred 1～2 hour are filtered to obtain filtrate for the first time; Extract for the second time crude drug and ethanol mass volume ratio g/ml is 1:10～1:20,60 ℃～70 ℃ of heated and stirred 30min～60min, filter to obtain filtrate for the second time; Filtrate and filtrate mixing for the second time, be concentrated into 0.2～0.5g/ml for the first time, obtains concentrate;
(3) be to add propylene glycol or butanediol in the concentrate that obtains to step (2) of 1:1～1:2 according to mass ratio, sucking filtration, 3000～5000 revs/min of centrifuging and taking supernatant, to obtain final product.
Wherein more preferably, in described step (2), extract for the first time crude drug and ethanol mass volume ratio g/ml is 1:20; 60 ℃ of heated and stirred 1 hour.
Wherein more preferably, in described step (2), extract for the second time crude drug and ethanol mass volume ratio g/ml is 1:10; 60 ℃ of heated and stirred 30 minutes.
There is an external application Chinese medicine extract for the white-skinned face function of releiving, extract and obtain by said method.
Above-mentioned external application Chinese medicine extract has the purposes in the skin care formulation of the white-skinned face function of releiving in preparation.
Have a skin care formulation for the white-skinned face function of releiving, described skin care formulation is made up of above-mentioned external application Chinese medicine extract and the conventional adjuvant of skin care field.
Have an external skin-care composition for the white-skinned face function of releiving, described external skin-care composition comprises A phase B phase, C phase and D phase, and described A, B, C, D are made up of the raw material of following weight percentage ratio:
A preparation method for above-mentioned external skin-care composition, comprises the steps:
(1) water is first heated to 40 ℃～50 ℃ dissolvings completely by two-PEG-18 methyl ether dimethylsilane, stand-by;
(2) A phase: add after water weighs up in water tank, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross linked polymer is evenly sprinkled upon to water surface, after soaking completely, add A other raw materials mutually, stir 30～50 revs/min, stir;
(3) in A phase suction emulsion tank step (2) being prepared, vacuum mix and blend, 30～50 revs/min of stir speed (S.S.)s, are heated to 80～85 ℃ of insulation 30～40min, stir cooling; Rate of temperature fall is 1～2 ℃/min;
(4) in the time that temperature drops to 45～50 ℃, add B phase NaOH, vacuum stirring is even;
(5) in the time that temperature drops to below 40 ℃, add C phase and D phase raw material, vacuum stirring is even, to obtain final product.
The preparation that above-mentioned raw materials and method prepare is essence.Utilize Chinese medicine composition in the present invention and skin care field conventional method and adjuvant also can prepare other dosage forms, as facial cream, cosmetic water, emulsion, spray etc.Chinese medicine composition in the present invention is simply pulverized and mixed and also can plays above-mentioned effect.
Essence of the present invention, recommendation method is: after clean of every day, essence is coated on skin, massage, treats that essence slowly absorbs gently.
The present invention confirms non-stimulated to skin safe through experiment, Chinese medicine extract itself not only has the effect of releiving, and also has white-skinned face function.Raw material of the present invention obtains easily, preparation method is easy, and green safety is easy to be accepted by irritated crowd, has good application prospect and market prospect.
Accompanying drawing explanation
Fig. 1 is the measurement result of Chinese medicine extract to hyaluronic acid enzyme inhibition rate;
Fig. 2 is the suppression ratio experiment of Chinese medicine extraction liquid to tryrosinase.
The specific embodiment
The present invention's medical material used all can be bought and obtain from China Medicament Group Corp., meets 2005 editions standards of Pharmacopoeia of People's Republic of China.Other raw materials all can obtain by commercially available purchase, and the present invention uses the source of raw material in table 1, and the instrument title that the present invention is used and producer are in table 2.
Table 1
| Title | Model | Producer |
| Ultraviolet-uisible spectrophotometer | UV2300 | Beijing Pu Xi all purpose instrument company limited |
| Ultrasound wave circulation extractor | SCIENTZ-HF5000 | Henan brother's instrument and equipment company limited |
| Dermal melanin and haemachrome tester | Mexameter?MX18 | CK electronics corporation of Germany |
| Refrigerated centrifuger | 4K15 | Sigma company |
| Homogenizer | BMS602 | BRT company |
Table 2
The preparation of embodiment 1 Chinese medicine composition of the present invention
(1) according to following weight parts proportioning weighting raw materials;
Herba Hedyotidis Diffusae 30g, Cortex Lycii 20g, Radix et Caulis Opuntiae Dillenii 10g, Radix Sophorae Flavescentis 20g, Folium Ginkgo 10g, Radix Glycyrrhizae 15g, Radix Et Rhizoma Fagopyri Tatarici 10g.
(2) percent by volume is 75% ethanol extraction twice, extracts for the first time crude drug and ethanol mass volume ratio g/ml is 1:10, and 60 ℃ of heated and stirred 1 hour are filtered to obtain filtrate for the first time; Extract for the second time crude drug and ethanol mass volume ratio g/ml is 1:10,60 ℃ of heated and stirred 30min, filter to obtain filtrate for the second time; Filtrate and filtrate mixing for the second time for the first time, rotary evaporation is concentrated into 0.2g/ml, obtains concentrate;
(3) be in the concentrate that obtains to step (2) of 1:2, to add propylene glycol to redissolve according to mass ratio, sucking filtration, 3000 revs/min of centrifuging and taking supernatant, to obtain final product.
The preparation of embodiment 2 Chinese medicine compositions of the present invention
(1) according to following weight parts proportioning weighting raw materials;
Herba Hedyotidis Diffusae 20g, Cortex Lycii 30g, Radix et Caulis Opuntiae Dillenii 20g, Radix Sophorae Flavescentis 10g, Folium Ginkgo 20g, Radix Glycyrrhizae 20g, Radix Et Rhizoma Fagopyri Tatarici 15g.
(2) percent by volume is 80% ethanol extraction twice, extracts for the first time crude drug and ethanol mass volume ratio g/ml is 1:20, and 70 ℃ of heated and stirred 2 hours are filtered to obtain filtrate for the first time; Extract for the second time crude drug and ethanol mass volume ratio g/ml is 1:20,70 ℃ of heated and stirred 60min, filter to obtain filtrate for the second time; Filtrate and filtrate mixing for the second time for the first time, rotary evaporation is concentrated into 0.3g/ml, obtains concentrate;
(3) be to add butanediol in the concentrate that obtains to step (2) of 1:1 according to mass ratio, sucking filtration, 5000 revs/min of centrifuging and taking supernatant, collect filtrate, to obtain final product.
The preparation of embodiment 3 Chinese medicine compositions of the present invention
(1) according to following weight parts proportioning weighting raw materials;
Herba Hedyotidis Diffusae 10g, Cortex Lycii 10g, Radix et Caulis Opuntiae Dillenii 15g, Radix Sophorae Flavescentis 15g, Folium Ginkgo 15g, Radix Glycyrrhizae 10g, Radix Et Rhizoma Fagopyri Tatarici 20g.
(2) percent by volume is 75% ethanol extraction twice, extracts for the first time crude drug and ethanol mass volume ratio g/ml is 1:15, and 65 ℃ of heated and stirred 1.5 hours are filtered to obtain filtrate for the first time; Extract for the second time crude drug and ethanol mass volume ratio g/ml is 1:15,65 ℃ of heated and stirred 40min, filter to obtain filtrate for the second time; Filtrate and filtrate mixing for the second time for the first time, rotary evaporation is concentrated into 0.5g/ml, obtains concentrate;
(3) be to add butanediol in the concentrate that obtains to step (2) of 1:1.5 according to mass ratio, sucking filtration, 4000 revs/min of centrifuging and taking supernatant, to obtain final product.
The preparation of embodiment 4 essences of the present invention
Raw material and proportioning (mass percent), in table 3:
Table 3
Essence preparation method:
(1) water is first heated to 40 ℃ of dissolvings completely by two-PEG-18 methyl ether dimethylsilane, stand-by;
(2) A phase: add after water weighs up in water tank, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross linked polymer is evenly sprinkled upon to water surface (noting not being spread across on pot wall), after soaking completely, adds A other raw materials mutually, stir 30 revs/min, stir;
(3), in A phase suction emulsion tank step (2) being prepared, 30 revs/min of vacuum mix and blends, are heated to 80 ℃ of insulation 30min, stir 30 revs/min of coolings; Rate of temperature fall is 1 ℃/min;
(4) in the time that temperature drops to 45 ℃, add B phase NaOH, 30 revs/min of vacuum stirring, stir;
(5) in the time that temperature drops to below 40 ℃, add C phase and D phase raw material, 30 revs/min of vacuum stirring, stir, and to obtain final product.
The preparation of embodiment 5 essences of the present invention
Raw material and proportioning (mass percent), in table 4:
Table 4
Essence preparation method:
(1) water is first heated to 50 ℃ of dissolvings completely by two-PEG-18 methyl ether dimethylsilane, stand-by;
(2) A phase: add after water weighs up in water tank, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross linked polymer is evenly sprinkled upon to water surface (noting not being spread across on pot wall), after soaking completely, adds A other raw materials mutually, stir 50 revs/min, stir;
(3), in A phase suction emulsion tank step (2) being prepared, 50 revs/min of vacuum mix and blends, are heated to 85 ℃ of insulation 40min, stir 30 revs/min of coolings; Rate of temperature fall is 2 ℃/min;
(4) in the time that temperature drops to 50 ℃, add B phase NaOH, 50 revs/min of vacuum stirring, stir;
(5) in the time that temperature drops to below 40 ℃, add C phase and D phase raw material, 50 revs/min of vacuum stirring, stir, and to obtain final product.
The preparation of embodiment 6 essences of the present invention
Raw material and proportioning (mass percent), in table 5:
Table 5
Essence preparation method:
(1) water is first heated to 45 ℃ of dissolvings completely by two-PEG-18 methyl ether dimethylsilane, stand-by;
(2) A phase: add after water weighs up in water tank, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross linked polymer is evenly sprinkled upon to water surface (noting not being spread across on pot wall), after soaking completely, adds A other raw materials mutually, stir 40 revs/min, stir;
(3), in A phase suction emulsion tank step (2) being prepared, 40 revs/min of vacuum mix and blends, are heated to 85 ℃ of insulation 35min, stir 40 revs/min of coolings; Rate of temperature fall is 1 ℃/min;
(4) in the time that temperature drops to 50 ℃, add B phase NaOH, 40 revs/min of vacuum stirring, stir;
(5) in the time that temperature drops to below 40 ℃, add C phase and D phase raw material, 40 revs/min of vacuum stirring, stir, and to obtain final product.
Effect experiment of the present invention
One, antianaphylaxis effect
1, experimental principle
This method adopts hyaluronidase vitro inhibition method to measure antiallergic effect of cosmetic material and cosmetic product.Hyaluronidase is anaphylactoid participant, hyaluronic acid (HA) in decomposer, make it become low-molecular-weight acid stimulus, cause histamine release, induction body produces responsive symptom, research shows that hyaluronidase and inflammation, allergy have strong correlation, and many Claritins have the effect of strong hyaluronidase inhibitor.
Hyaluronidase can be hydrolyzed 1 between β-N-acetyl-glucosamine in hyaluronic acid and D-glucuronate indiscriminately, 4-key, and obtain β-N-acetyl-glucosamine, β-N-acetyl-glucosamine under alkali condition can with the acetylacetone,2,4-pentanedione condensation former 2-methyl-3-diacetyl azole derivatives that adds lustre to, the former and p-dimethylaminobenzaldehyde that adds lustre to adds lustre in concentrated hydrochloric acid ethanol.
2, experiment content
Adopt hyaluronidase body outer suppressioning experiment Elson-Morgan method to carry out.Get 0.1mL0.25mmol/LCaCl
2solution and 37 ℃ of heat insulating culture 20min of 0.5mL hyaluronidase liquid; Add sample liquid 0.5mL, continue 37 ℃ of heat insulating culture 20min; Add 37 ℃ of insulation 30min of 0.5mL hyaluronate sodium liquid, room temperature is placed 5min; Add 0.1mL0.4mol/LNaOH solution and 0.5mL acetylacetone,2,4-pentanedione solution, be placed in after boiling water bath heats 15min and carry out cooling 5min with frozen water immediately; Add Ehrlich's reagent 1.0mL also to dilute with 3.0mL dehydrated alcohol, place 20min colour developing, with its absorbance of spectrophotometric determination.
Antiallergic activity computing formula:
In formula: A---contrast solution ABS value (replacing sample solution with hac buffer)
B---contrast blank solution ABS value (with hac buffer replacement sample solution and enzyme liquid)
C---sample solution ABS value
D---sample blank solution A BS value (with hac buffer replacement enzyme liquid)
The length scanning that first A group sample is carried out 450-700nm scope when experiment, to determine maximum absorption wavelength, then using deionized water as reference, carries out respectively ABS pH-value determination pH at this maximum absorption wavelength place.
The difference of twice measured value of same sample should be no more than 10% of twice mensuration meansigma methods.
Sample is embodiment 1 Chinese medicine extract.
3, interpretation of result
Experiment the sample that adopts hyaluronic acid enzyme inhibition rate measurement result as shown in Figure 1, the aqueous solution that test concentrations is 1.0%, data are three meansigma methodss.Analyze: wherein positive control is antipruritic and antipruritic agent.Suppression ratio is in effective range as seen from Figure 1, and the suppression ratio of sample is (0-100%) in effective range, has the effect that suppresses hyaluronidase, and larger, effect better (effective range 0-100%).Therefore, in embodiment 1, the Chinese medicine extract of preparation has antiallergic effect.Inventor repeats above-mentioned experimental result with above-mentioned with the Chinese medicine extract of embodiment 2,3 preparations and the essence of embodiment 4,5,6 preparations.
Two, tryrosinase suppresses experiment
In melanic forming process, tryrosinase has played key enzyme, melanin produce number and the activity of tryrosinase have direct relation.Therefore, skin-whitening agents generates by acting on dermal melanin exactly, and in metabolic process, check melanin generates and meet the material of safety standard.Can there is catalytic reaction in L – tryrosinase and its substrate L – tyrosine.Have after the inhibiting reagent of L – tyrosinase activity when having added in experimental system, can produce inhibitory action to catalytic reaction, add reagent front and back in the absorption photometric at 475nm place by mensuration, evaluate the suppression ratio of reagent to L – tyrosinase activity.
Test required solution preparation:
A: accurately take 17.91g disodium hydrogen phosphate and be dissolved in distilled water, with 500mL volumetric flask standardize solution;
B: accurately take 7.8g sodium dihydrogen phosphate dihydrate and be dissolved in distilled water, with 500mL volumetric flask standardize solution;
C: get solution 92.6mL in a, solution 107.4mL in b, is made into 200mLpH=6.8PBS buffer solution.
D:0.05%L – tyrosine solution: accurately take 0.05gL-tyrosine, first use the dissolving with hydrochloric acid of a small amount of 0.1mol/L, be settled to 100mL with the phosphate buffer solution (PBS) of pH=6.8 after it dissolves.
Table 6 white-skinned face function test experience preparation table
Note: C1 and T1 add 0.5mL tryrosinase, and enzyme is lived as 100U/mL.
Experimental procedure:
(1), after C2 pipe prepares and shakes up, heating in water bath 10min in 37 ℃ of water-baths, returns to zero under wavelength 475nm.
(2) C1 pipe solution prepares and shakes up, and after 37 ℃ of water-bath 10min, adds tryrosinase 0.5ml, continues water-bath 10 minutes, measures C1 absorbance.
(3), by (1) (2) same method, measure T1 absorbance with T2 zeroing.
(4) the maximum inhibition T(% of calculation sample to tryrosinase).
Formula: T(%)=(C1-T1)/C1 × 100%
What sample used is the Chinese medicine extract of preparation in embodiment 1.
2, the embodiment 1 that the results are shown in Figure that tryrosinase suppresses experiment prepares the increase of extract along with addition concentration, and suppression ratio improves gradually, proves that this extract has white-skinned face function.Extract is along with the increase of addition concentration, and suppression ratio improves gradually, proves that this extract has white-skinned face function.Inventor repeats above-mentioned experimental result with above-mentioned with the Chinese medicine extract of embodiment 2,3 preparations and the essence of embodiment 4,5,6 preparations.
Three, itching-relieving efficacies research---histamine phosphate causes the method for itching
1, experiment material
Laboratory animal: 96 of Cavia porcelluss, male and female half and half, 350 ± 20g.
Given the test agent: embodiment 4 essence high dose group are 0.8g; Middle dosage group is 0.4g; Low dose group is 0.2g.
Positive control: fluocinonide ointment (1.0g/Kg).
2, experiment reagent
The main experiment reagent summary sheet of table 7
3, experimental apparatus
The main experimental apparatus summary sheet of table 8
4, experimental technique
A. get 32 of Cavia porcelluss, shave hair in first 1 day right back instep of experiment, be divided at random 4 groups.Be sample sets, positive controls, distilled water negative control group, Normal group.
B. within continuous 2 days, evenly smear respectively corresponding dosage sample, positive reference substance and distilled water in shaving hair place, Normal group is left intact.
C. test the 3rd day, it is appropriate that precision takes histamine phosphate, is made into 0.01%, 0.02%, 0.03%, 0.04% before use with distilled water ... gradient concentration is for subsequent use.With coarse sandpaper, hair place is shaved in right back Cavia porcellus instep and abrade, the about 1cm of area
2, part repastes medicine 1 time, after last coating 10min, drips 0.01% histamine phosphate 0.05mL at abrasion, complies with 0.01%, 0.02%, 0.03%, 0.04% every 3 minutes later ... progressive concentration is 0.05mL at every turn.Until occur that Cavia porcellus later licks right back foot, finally to occur that histamine phosphate total amount that Cavia porcellus later licks right back sufficient time institute's fine-still is as itch-threshold.
D. calculate the diversity between each group of itch-threshold comparable group.
What sample used is essence prepared by embodiment 4.
Table 9 essence causes the impact (x ± s, n=6) of the reaction of itching on Cavia porcellus histamine phosphate
Note: with model control group comparison, * p<0.05, * * p<0.01
As seen from the above table, compare with model group, fluocinolone acetonide ointment causes to Cavia porcellus histamine phosphate the reaction of itching extremely significant inhibitory action (P<0.01), and sample sets also has significant inhibitory action (P<0.05).Inventor repeats above-mentioned experimental result with above-mentioned with the essence of embodiment 5,6 preparations.
Four, the repair of essence to skin
A. get 32 of Cavia porcelluss, be divided at random 4 groups.Be sample sets, positive controls, distilled water negative control group, Normal group.
B. l days before experiment, by 6 treated animal etherizations, cuts neck back wool, and area is 2 × 2cm
2.
C. get acetone: ether=1:1 mixed liquor 150 μ L drip in unhairing place, then get respectively corresponding sample, positive reference substance and distilled water 150 μ L and drip in cropping place, 2 times/day, Normal group is left intact.
D. measured its depilation place transepidermal water loss rate in the 5th day, the diversity of relatively more each group.
What sample used is essence prepared by embodiment 4.
Table 10 to allergy after the repair of skin
Note: with model control group comparison, * p<0.05, * * p<0.01
As seen from the above table, compare with model group, sample sets has extremely significant repair (P<0.01) to allergic skin, and fluocinolone acetonide ointment has significant repair (P<0.05).Inventor repeats above-mentioned experimental result with above-mentioned with the essence of embodiment 5,6 preparations.
Claims (10)
1. there is an external medicine composition for the white-skinned face function of releiving, it is characterized in that being made by the crude drug of following weight parts proportioning:
Herba Hedyotidis Diffusae 10～30, Cortex Lycii 10～30, Radix et Caulis Opuntiae Dillenii 10～20, Radix Sophorae Flavescentis 10～20, Folium Ginkgo 10～20, Radix Glycyrrhizae 10～20, Radix Et Rhizoma Fagopyri Tatarici 10～20.
2. external medicine composition as claimed in claim 1, is characterized in that, described compositions is made up of the crude drug of following weight parts proportioning:
Herba Hedyotidis Diffusae 20～30, Cortex Lycii 20～30, Radix et Caulis Opuntiae Dillenii 15～20, Radix Sophorae Flavescentis 15～20, Folium Ginkgo 10～15, Radix Glycyrrhizae 10～15, Radix Et Rhizoma Fagopyri Tatarici 10～15.
3. a preparation method for external medicine composition described in claim 1 or 2, is characterized in that comprising the steps:
(1) according to weight portion proportioning weighting raw materials described in claim 1 or 2;
(2) percent by volume is 75%～80% ethanol extraction twice, extracts for the first time crude drug and ethanol mass volume ratio g/ml is 1:10～1:20, and 60 ℃～70 ℃ heated and stirred 1～2 hour are filtered to obtain filtrate for the first time; Extract for the second time crude drug and ethanol mass volume ratio g/ml is 1:10～1:20,60 ℃～70 ℃ of heated and stirred 30min～60min, filter to obtain filtrate for the second time; Filtrate and filtrate mixing for the second time, be concentrated into 0.2～0.5g/ml for the first time, obtains concentrate;
(3) be to add propylene glycol or butanediol in the concentrate that obtains to step (2) of 1:1～1:2 according to mass ratio, sucking filtration, 3000～5000 revs/min of centrifuging and taking supernatant, to obtain final product.
4. preparation method as claimed in claim 3, is characterized in that, extracts for the first time crude drug and ethanol mass volume ratio g/ml is 1:20 in described step (2); 60 ℃ of heated and stirred 1 hour.
5. preparation method as claimed in claim 3, is characterized in that, extracts for the second time crude drug and ethanol mass volume ratio g/ml is 1:10 in described step (2); 60 ℃ of heated and stirred 30 minutes.
6. the tool external application Chinese medicine extract for white-skinned face function of releiving, is characterized in that, described external application Chinese medicine extract is extracted and to be obtained by method described in any one in claim 3 to 5.
7. described in claim 6, external application Chinese medicine extract has the purposes in the external-use skin care preparation of the white-skinned face function of releiving in preparation.
8. a skin care formulation with the white-skinned face function of releiving, is characterized in that, described skin care formulation is made up of external application Chinese medicine extract described in claim 6 and the conventional adjuvant of skin care field.
9. an external skin-care composition with the white-skinned face function of releiving, is characterized in that, described external skin-care composition comprises A phase B phase, C phase and D phase, and described A, B, C, D are made up of the raw material of following weight percentage ratio:
10. a preparation method for external skin-care composition described in claim 9, is characterized in that comprising the steps:
(1) water is first heated to 40 ℃～50 ℃ dissolvings completely by two-PEG-18 methyl ether dimethylsilane, stand-by;
(2) A phase: add after water weighs up in water tank, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross linked polymer is evenly sprinkled upon to water surface, after soaking completely, add A other raw materials mutually, stir 30～50 revs/min, stir;
(3) in A phase suction emulsion tank step (2) being prepared, vacuum mix and blend, 30～50 revs/min of stir speed (S.S.)s, are heated to 80～85 ℃ of insulations 30～40 minutes, stir cooling;
(4) in the time that temperature drops to 45～50 ℃, add B phase NaOH, vacuum stirring is even;
(5) in the time that temperature drops to below 40 ℃, add C phase and D phase raw material, vacuum stirring is even, to obtain final product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105581937A (en) * | 2015-10-10 | 2016-05-18 | 北京工商大学 | External use plant composition with skin whitening effect, preparation and preparing method of external use plant composition and preparing method of external use plant composition preparation |
| CN106166256A (en) * | 2016-08-06 | 2016-11-30 | 北京国济众芳中医药研究所 | There is the external medicine composition of antiallergic itching-relieving efficacies, preparation and preparation method thereof |
| CN106562913A (en) * | 2015-10-10 | 2017-04-19 | 孟宏 | An externally applied composition and cosmetic preparation having whitening effects and preparing methods thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101732205A (en) * | 2009-12-29 | 2010-06-16 | 北京工商大学 | Skin-care composition and preparation for relieving skin allergies and resisting inflammatory and irritation and preparation method thereof |
| CN102247305A (en) * | 2010-05-18 | 2011-11-23 | 北京工商大学 | Extract capable of inhibiting ultraviolet ray damages on skin, and its preparation method and use |
-
2014
- 2014-01-15 CN CN201410018343.0A patent/CN103800559B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101732205A (en) * | 2009-12-29 | 2010-06-16 | 北京工商大学 | Skin-care composition and preparation for relieving skin allergies and resisting inflammatory and irritation and preparation method thereof |
| CN102247305A (en) * | 2010-05-18 | 2011-11-23 | 北京工商大学 | Extract capable of inhibiting ultraviolet ray damages on skin, and its preparation method and use |
Non-Patent Citations (6)
| Title |
|---|
| JEONGCHEOL HA等: ""含白花蛇舌草提取物的化妆品美白功效研究"", 《第八届中国化妆品学术研讨会论文集》 * |
| 刘德军: "《现代中药化妆品制作工艺及配方》", 28 February 2009 * |
| 曾鸣等: ""化妆品用植物提取物美白潜力评估的研究进展"", 《日用化学工业》 * |
| 王建新: "《化妆品天然功能成分》", 31 January 2007 * |
| 董银卯等: ""中医药理论与技术在化妆品中的应用"", 《日用化学品科学》 * |
| 董银卯等: "《本草药妆品》", 30 June 2010 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105581937A (en) * | 2015-10-10 | 2016-05-18 | 北京工商大学 | External use plant composition with skin whitening effect, preparation and preparing method of external use plant composition and preparing method of external use plant composition preparation |
| CN106562913A (en) * | 2015-10-10 | 2017-04-19 | 孟宏 | An externally applied composition and cosmetic preparation having whitening effects and preparing methods thereof |
| CN105581937B (en) * | 2015-10-10 | 2018-01-12 | 北京工商大学 | Exterior-applied plant composition, preparation with white-skinned face function and preparation method thereof |
| CN106562913B (en) * | 2015-10-10 | 2020-07-28 | 孟宏 | External composition with whitening effect, cosmetic preparation and preparation method thereof |
| CN106166256A (en) * | 2016-08-06 | 2016-11-30 | 北京国济众芳中医药研究所 | There is the external medicine composition of antiallergic itching-relieving efficacies, preparation and preparation method thereof |
| CN106166256B (en) * | 2016-08-06 | 2019-09-20 | 北京国济众芳中医药研究所 | External medicine composition, preparation with antiallergic itching-relieving efficacies and preparation method thereof |
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