CN103688624B - Method for promoting quick sprouting of Chinese rose seeds - Google Patents
Method for promoting quick sprouting of Chinese rose seeds Download PDFInfo
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- CN103688624B CN103688624B CN201310731663.6A CN201310731663A CN103688624B CN 103688624 B CN103688624 B CN 103688624B CN 201310731663 A CN201310731663 A CN 201310731663A CN 103688624 B CN103688624 B CN 103688624B
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- 238000000034 method Methods 0.000 title claims abstract description 98
- 240000008254 Rosa chinensis Species 0.000 title claims abstract description 31
- 235000000664 Rosa chinensis Nutrition 0.000 title claims abstract description 31
- 230000001737 promoting effect Effects 0.000 title abstract description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 22
- 108010059892 Cellulase Proteins 0.000 claims abstract description 14
- 229940106157 cellulase Drugs 0.000 claims abstract description 14
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 12
- 239000004576 sand Substances 0.000 claims abstract description 12
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 11
- 239000003448 gibberellin Substances 0.000 claims abstract description 11
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910000029 sodium carbonate Inorganic materials 0.000 claims abstract description 6
- 230000035784 germination Effects 0.000 claims description 15
- 230000032823 cell division Effects 0.000 claims description 8
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 6
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 6
- 239000004571 lime Substances 0.000 claims description 6
- 229910002651 NO3 Inorganic materials 0.000 claims description 5
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 5
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 3
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 238000012545 processing Methods 0.000 abstract description 7
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 abstract description 4
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 abstract description 4
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract 1
- 239000004062 cytokinin Substances 0.000 abstract 1
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 6
- CGIDKJRJBMFXKV-UHFFFAOYSA-N 6-n'-benzylpurine-6,6-diamine Chemical compound N1=CN=C2N=CN=C2C1(N)NCC1=CC=CC=C1 CGIDKJRJBMFXKV-UHFFFAOYSA-N 0.000 description 5
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 5
- 230000007226 seed germination Effects 0.000 description 5
- 239000011575 calcium Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 235000011449 Rosa Nutrition 0.000 description 2
- 241000220317 Rosa Species 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- JLIDBLDQVAYHNE-LXGGSRJLSA-N 2-cis-abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\C1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-LXGGSRJLSA-N 0.000 description 1
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 1
- 108010082126 Alanine transaminase Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000035584 blastogenesis Effects 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000026267 regulation of growth Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for promoting quick sprouting of Chinese rose seeds; the method comprises sand burying, concentrated hydrochloric acid processing, thermokalite (sodium carbonate) processing, cellulase processing, gibberellin processing, cytokinin processing and calcium nitrate processing. The method can improve the sprouting rate of the Chinese rose seeds by 3-5 times, can prominently improve the sprouting uniformity, has wide application foreground, and has important significance on the promotion of the cultivation of novel varieties of Chinese roses and the breeding of stocks.
Description
Technical field
The present invention relates to a kind of method promoting seed sprouting, particularly a kind of method promoting Chinese rose seed fast germination.
Background technology
Chinese rose (Rosa chinensis) calls perpetual bloom, Chinese rose, belongs to evergreen or half evergreen shrubs of rose family Rosa.Modern rose cultivars is various, pattern is gorgeous, the fragrance of a flower is strong fragrant, flower pattern is graceful, in extensively distribution and cultivation all over the world, it is collection greening, view and admire, health care, eat the flower variety in one, the favor of people is all over the world subject to many fine qualities, be described as " spending middle queen ", that numerous domestic city is as Tianjin, Beijing, Shijiazhuang, Zhengzhou, Nanchang, Dalian, Changzhi, Yuncheng, Changzhou, Anqing, Yichang, Ji'an, Jiaozhuo, table mountain, Huaian, Hengyang, Yingtan, Qingdao, Weifang, Xingtai, Cangzhou, Langfang, Deyang, the city flower in the cities such as Xinyang, in afforestation, there is good landscape effect.By the definition of 1966 of Chinese rose association of the U.S., ' La France ' occurs that later Breeds is called modern distance education system to the fragrant Chinese rose of first hybrid tea in 1867, and the kind before 1867 is referred to as ancient Chinese rose.Now for the production of and gardens, cut-flower application be modern distance education system mostly.Modern distance education system is repeatedly hybridized, through the everblooming Hybrid group of long-term breeding between planting in Rosa.About there are 200 kinds of guls (original China accounts for 41% of sum) in the whole world, but in the past in rose breeding, 200 process, create more than 25000 modern distance education system kind, create the miracle on flower breeding, the aspects such as pattern, flower pattern, plant type, continuously blooming property be all other flowers incomparable.
Along with the effect of Chinese rose in the industry of flowers and plants becomes increasingly conspicuous, utilization and the rearing new variety of Chinese rose resource are paid attention to day by day.And seminal propagation is the main method of cultivating new varieties and breeding stock.But Chinese rose seed seed coat is hard, have confinement effect to the absorption of moisture and respiratory metabolism, and pericarp, seed coat, in embryo all containing abscisic acid (ABA), have the characteristic of deep-sleep, therefore germination rate is general very low and irregular.For improving breeding efficiency and resource utilization, need to process seed, conventional method for treating seeds safety is lower, and treatment effect is undesirable.
The present invention by Seed Germination Test, the method adopting physics and chemistry process to combine, buried by sand, concentrated hydrochloric acid, thermokalite, cellulase, gibberellin, basic element of cell division process, preferred process combines, and solves the problem that Chinese rose seed germination rate is low.
Summary of the invention
The object of this invention is to provide a kind of method promoting Chinese rose seed fast germination, the method comprises the steps such as sand buries, concentrated hydrochloric acid process, thermokalite (sodium carbonate) process, cellulase process, gibberellin process, basic element of cell division process, nitrate of lime process.Facts have proved, use the method that Chinese rose percentage of seedgermination can be made to improve 3-5 doubly, germination regularity can be significantly improved simultaneously, have broad application prospects, for the breeding of the cultivation and stock that promote Chinese rose new varieties has great importance.
The technical solution used in the present invention is:
Promote a method for Chinese rose seed fast germination, the method comprises: sand buries, concentrated hydrochloric acid process, thermokalite (sodium carbonate) process, cellulase process, gibberellin process, basic element of cell division process, nitrate of lime process.
Further, the method for this promotion Chinese rose seed fast germination, the method comprises the steps:
1), sand buries: mixed with wet sand by Chinese rose seed, after plastic bag sealing, be placed in 2-5 DEG C of refrigerator low temperature treatment, take out and clean after 30d;
2), concentrated hydrochloric acid process: by through step 1) seed after process is soaked in concentrated hydrochloric acid, process 20min, takes out and cleans;
3), sodium carbonate process: by through step 2) seed after process is soaked in Na
2cO
3in solution, constantly stir, process 10min, takes out and cleans;
4), cellulase process: by through step 3) seed after process is immersed in cellulase solution, process 48h, takes out and cleans;
5), gibberellin process: by through step 4) seed after process is soaked in Gibberellins solution, process 36h, takes out and cleans;
6), basic element of cell division process: by through step 5) seed after process is soaked in 6-BA solution, process 24h, takes out and cleans.
7), nitrate of lime process: by through step 6) seed after process is soaked in Ca (NO
3)
2in solution, process 24h, takes out and cleans.
More specifically, the method for promotion Chinese rose seed fast germination of the present invention, comprises the steps:
1), sand buries: mixed with wet sand by Chinese rose seed, wet husky water content is about 40%, after plastic bag sealing, is placed in 2-5 DEG C of refrigerator low temperature treatment, takes out and clean after 30d;
2), concentrated hydrochloric acid process: by through step 1) seed after process is soaked in concentrated hydrochloric acid, process 20min, takes out and cleans;
3), sodium carbonate process: by through step 2) seed after process is soaked in the Na of the concentration 1% of 75 DEG C
2cO
3in solution, constantly stir, process 10min, takes out and cleans;
4), cellulase process: by through step 3) seed after process is immersed in the cellulase solution of concentration 5%, 25 DEG C by day, night processes 48h under the room temperature condition of 15 DEG C, take out and clean;
5), gibberellin process: by through step 4) seed after process is soaked in the Gibberellins solution of 100mg/L, 25 DEG C by day, night processes 36h under the room temperature condition of 15 DEG C, take out and clean;
6), basic element of cell division process: by through step 5) seed after process is soaked in the 6-BA solution of 20mg/L, 25 DEG C by day, night processes 24h under the room temperature condition of 15 DEG C, take out and clean.
7), nitrate of lime process: by through step 6) seed after process is soaked in the Ca (NO of 10mmol/L
3)
2in solution, 25 DEG C by day, night processes 24h under the room temperature condition of 15 DEG C, take out and clean.
The Na of the concentration 1% mentioned in said method
2cO
3the concentration involved by cellulase solution of solution, concentration 5% is mass concentration.
In said method, step 1)-7) " take out and clean " of mentioning be and clean with distilled water.
In said method, each material mentioned and effect thereof are respectively:
Cellulase is extensively present in natural organism, is a kind of complex enzyme, and one that is made up of multiple hydrolase complicated enzyme system, plays biocatalysis when decomposition of cellulose, cellulose decomposition can be become glucose; Bacterium, fungi, cellulase can be produced in animal body etc.
Gibberellin (GA) is the class plant hormone extensively existed, can growth regulation and the various growth course of impact, and comprise the prolongation promoting stem, germinate, seed dormancy, blooms, Sex, the aging suppression of leaf and fruit.
The basic element of cell division, i.e. 6-benzyl aminoadenine (6-BA), it is the plant growth regulator to person poultry safety, mainly be distributed in and carry out fissional position, as inner in the seed of stem apex, the tip of a root, immature seed, sprouting, the fruit that grows, can promote cell division, promote seed sprouting, induced dormancy blastogenesis is long.
Calcium ion is the necessary mineral nutrient element of plant growth, is also a kind of important Metabolism regulation material.The interpolation of External Calcium nitrate of lime can improve the content of soluble sugar in endosperm, can also improve the activity of amylase in Seed Germination, lipase, glutamic-pyruvic transaminase, and then promotes the sprouting of seed.
The beneficial effect that the present invention has:
The inventive method can make Chinese rose percentage of seedgermination improve 3-5 doubly, can significantly improve germination regularity simultaneously, have broad application prospects, for the breeding of the cultivation and stock that promote Chinese rose new varieties has great importance.
Embodiment
Below by specific embodiment, the invention will be further described, but do not limit protection scope of the present invention.
1 gets 800 Chinese rose " spectrum " seeds, and sand buries process.Mixed with wet sand by Chinese rose seed, wet husky water content is about 40%, after plastic bag sealing, is placed in 2-5 DEG C of refrigerator low temperature treatment, takes out and clean after 30d;
2 get wherein 400 with concentrated hydrochloric acid process 20min, take out and clean; Then the Na of 75 DEG C 1% is used
2cO
3solution-treated 10min, takes out and cleans; Then with cellulase solution 25 DEG C by day, the night of 5% processing 48h under the room temperature condition of 15 DEG C, taking out and cleaning; Then the Gibberellins solution of 100mg/L 25 DEG C by day, night process 36h under the room temperature condition of 15 DEG C, take out and clean; Then the 6-BA solution of 20mg/L 25 DEG C by day, night process 24h under the room temperature condition of 15 DEG C, take out and clean; Then Ca (the NO of 10mmol/L
3)
2solution-treated 24h, takes out and cleans; After being disposed, get wherein 300 carry out sprouting experiment, survey ABA content for other 100.
3 other 400 seeds as a control group, are got 300 and are carried out sprouting experiment, survey ABA content for 100.
Control group 45d germination rate is 12%, ABA content is 0.798 μ g/g, and experimental group 15d germination rate is 51%, ABA content is 0.211 μ g/g.The method of visible promotion Chinese rose seed fast germination provided by the present invention significantly can reduce the content of seed ABA, improves germination rate and germination regularity.
The above is only preferred embodiment of the present invention, not does any pro forma restriction to technical scheme of the present invention.Every above embodiment is done according to technical spirit of the present invention any simple modification, equivalent variations and modification, all still belong in the scope of technical scheme of the present invention.
Claims (1)
1. promote a method for Chinese rose seed fast germination, it is characterized in that: comprise the steps:
1), sand buries: mixed with wet sand by Chinese rose seed, wet husky water content is 40%, after plastic bag sealing, is placed in 2-5 DEG C of refrigerator low temperature treatment, takes out and clean after 30d;
2), concentrated hydrochloric acid process: by through step 1) seed after process is soaked in concentrated hydrochloric acid, process 20min, takes out and cleans;
3), sodium carbonate process: by through step 2) seed after process is soaked in the Na of the concentration 1% of 75 DEG C
2cO
3in solution, constantly stir, process 10min, takes out and cleans;
4), cellulase process: by through step 3) seed after process is immersed in the cellulase solution of concentration 5%, 25 DEG C by day, night processes 48h under the room temperature condition of 15 DEG C, take out and clean;
5), gibberellin process: by through step 4) seed after process is soaked in the Gibberellins solution of 100mg/L, 25 DEG C by day, night processes 36h under the room temperature condition of 15 DEG C, take out and clean;
6), basic element of cell division process: by through step 5) seed after process is soaked in the 6-BA solution of 20mg/L, 25 DEG C by day, night processes 24h under the room temperature condition of 15 DEG C, take out and clean;
7), nitrate of lime process: by through step 6) seed after process is soaked in the Ca (NO of 10mmol/L
3)
2in solution, 25 DEG C by day, night processes 24h under the room temperature condition of 15 DEG C, take out and clean.
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|---|---|---|---|
| CN201310731663.6A CN103688624B (en) | 2013-12-18 | 2013-12-18 | Method for promoting quick sprouting of Chinese rose seeds |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310731663.6A CN103688624B (en) | 2013-12-18 | 2013-12-18 | Method for promoting quick sprouting of Chinese rose seeds |
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| Publication Number | Publication Date |
|---|---|
| CN103688624A CN103688624A (en) | 2014-04-02 |
| CN103688624B true CN103688624B (en) | 2015-02-25 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104956809A (en) * | 2015-07-10 | 2015-10-07 | 天津泰达绿化集团有限公司 | Method for treating sesbania seeds before sowing |
| CN105027733B (en) * | 2015-07-28 | 2017-06-27 | 平顶山学院 | A kind of method promoting the seed germination of southern yew |
| CN109548584B (en) * | 2019-01-16 | 2021-07-06 | 山东农业大学 | A method for promoting reed seed germination and improving salt tolerance |
| CN113424738A (en) * | 2021-06-03 | 2021-09-24 | 安徽农之源生态农业有限公司 | Method for promoting rapid germination of Chinese rose seeds |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101663934B (en) * | 2009-09-22 | 2011-05-11 | 四川大学 | Regulating and controlling method for improving germination rate of woody seeds |
| CN102652469A (en) * | 2012-05-11 | 2012-09-05 | 句容谷歌庄园现代农业科技发展有限公司 | High-efficiency seedling method for goldenrain tree |
| CN102696301B (en) * | 2012-06-29 | 2013-09-18 | 中南林业科技大学 | Method for enhancing germination rate of litsea cubeba seeds |
| CN103141181B (en) * | 2013-03-21 | 2015-03-18 | 云南省农业科学院花卉研究所 | Method for promoting rosa chinensis seed to sprout rapidly |
| CN103355084B (en) * | 2013-07-30 | 2014-10-29 | 云南省农业科学院花卉研究所 | Method for promoting sprouting of rosa praelucens seeds |
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Address after: No. 26 Muning Road, Economic and Technological Development Zone, Binhai New Area, Tianjin, 300457 Patentee after: Tianjin TEDA Green Technology Group Co.,Ltd. Address before: No. 26 Muning Road, Economic and Technological Development Zone, Binhai New Area, Tianjin, 300457 Patentee before: TIANJIN TEDA GREENING GROUP Co.,Ltd. |
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Address after: No. 26 Muning Road, Economic and Technological Development Zone, Binhai New Area, Tianjin, 300457 Patentee after: Tianjin TEDA Green Technology Group Co.,Ltd. Address before: No. 26 Muning Road, Economic and Technological Development Zone, Binhai New Area, Tianjin, 300457 Patentee before: Tianjin TEDA Green Technology Group Co.,Ltd. |