CN107624602A - A kind of cuttage breeding method of Ranunculus asiaticus - Google Patents
A kind of cuttage breeding method of Ranunculus asiaticus Download PDFInfo
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- CN107624602A CN107624602A CN201711131984.7A CN201711131984A CN107624602A CN 107624602 A CN107624602 A CN 107624602A CN 201711131984 A CN201711131984 A CN 201711131984A CN 107624602 A CN107624602 A CN 107624602A
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Abstract
The invention provides a kind of cuttage breeding method of Ranunculus asiaticus, belongs to Ranunculus asiaticus reproduction technique field, comprises the following steps：Greenhouse is built, is separated in greenhouse with rib and does some seedling ponds, flat surface；In the dormancy period of Ranunculus asiaticus, choose the raw then, spray of robust growth, sturdy position is chosen after cutting, be cut into 10 ~ 12 centimeter lengths one section is used as cuttage branch, every section of at least two eyes of band, by cuttage branch first use mass percent for 0.05 ~ 0.10% disinfecting solution of potassium permanganate, soaked 10 ~ 15 minutes with the peptide pyridimine nucleosides antibiotic aqua that mass percent is 3 ~ 4% again, after taking-up cuttage branch is hung, cuttage part is immersed in compounding nutrient solution, stand 8 ~ 10 hours, take out, Miao Chizhong is entered in cuttage, after treating cuttage, Miao Chi waters, and seals canopy；Cultivate.The inventive method cuttage is easy, and cuttage survival rate is high, and lifting does not hinder root, and transplanting survival rate is high.
Description
Technical field
The invention belongs to Ranunculus asiaticus reproduction technique field, and in particular to a kind of cuttage breeding method of Ranunculus asiaticus.
Background technology
Ranunculus asiaticus (Ranunculus asianticus L.), also known as celery flower, Persian buttercup, Kunming Areas are commonly called as " ocean
Tree peony ", it is Ranunculaceae Ranunculus asiaticus category perennial herb flowers.Ranunculus asiaticus strain appearance is exquisite beautiful, spends large-scale U.S., wide in variety
Sample, pattern enrich gorgeous, there is the color such as white, powder, Huang, red, purple, polyphyll or half polyphyll, 20 ~ 40cm of plant height, root tuber spindle, constant
Individual consor is in root neck；Stem Dan Sheng, or a small number of branches, hairiness；Basal leaf is wealthy avette, has long handle, stem leaf stockless, is answered for pinniform
Leaf；Hua Dansheng or several basidixed, 3 ~ 4cm of Hua Jing；4 ~ May of florescence.
Ranunculus asiaticus is a kind of flowers type of great growth potential, in recent years due to the successful choosing of first-filial generation new varieties
Educate, further enrich designs and varieties, improve its status and influence power in flowers family, the productive consumption of Ranunculus asiaticus obtains
To developing on an unprecedented scale, seedling demand is rapidly expanded.
Publication No. CN1613298A patent discloses a kind of tissue culture and rapid proliferation method of Ranunculus asiaticus, and it is adopted
With techniques such as bulb vernalization, explant sterilizing, adventitious bud induction culture, adventitious bud squamous subculture, culture of rootage and transition cultivatings
Step, and cultivated on corresponding each culture medium, until directly transplanting after growing up to rooted seedling.It this method solve
Ranunculus asiaticus sterile system establish key technology, while by hormone, nutrition, culture environment comprehensive adjustment, reached enter kind
Efficiency high, growth rate is fast, the stable purpose of production technology, realizes the large-scale production of Ranunculus asiaticus improved seeds.The breeding side
Method cost is high, easy hydridization, quality are unstable, it is difficult to meets Ranunculus asiaticus standardization, high-quality, the demand for development of multi-quantity, seriously
Constrain the business development and application of Ranunculus asiaticus.
Publication No. CN104620816A patent discloses a kind of Ranunculus asiaticus non-woven bag seedling cultural method, and it is included such as
Lower step：1) seed is sowed；2) treat that seedling is grown to 3-4 piece true leaves, during height of seedling 5-7cm, by seedling seedling separation to adaptable with it
In soluble non-woven fabrics pocket, non-woven fabrics pocket is colonized in Seedlings nursery；3) go out non-woven bag seedling after seedling separation from 45 days, press
20-30cm × 20-30cm seeding row spacing is colonized, in growth medium height of seedling 10-15cm, concentration 3000mg/kg
B9 carry out foliage-spray, be spaced January after reuse once.The operation of this method lifting is easy, not fugitive top, piles up simplicity, can
Adapt to relatively long distance transport and jolt；Plant is easy, easy to operate；Non-woven fabrics directly decomposes, and does not produce rubbish；Do not have to after construction
Slow seedling, fast rapid-result scape effect is good, wounded in the battle can transplant, horse back Cheng Jing.Seminal propagation due to percentage of seedgermination it is low (normally only 20%~
40%), kind property is unstable, kind dissociation is big, and production technology operation difficulty is larger.
The content of the invention
In view of this, it is an object of the invention to provide a kind of cuttage breeding method of Ranunculus asiaticus.
To achieve the above object, the technical solution adopted in the present invention is：A kind of cuttage breeding method of Ranunculus asiaticus, including
Following steps：
Step S1：Build greenhouse, separated in the greenhouse with rib and do some seedling ponds, in the seedling pond by it is bottom up according to
It is secondary uniformly to lay decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa, flat surface；
Step S2：In the dormancy period of Ranunculus asiaticus, the raw then, spray of robust growth is chosen, sturdy position is chosen after cutting, is cut
Cuttage branch is used as into one section of 10 ~ 12 centimeter lengths, every section of at least two eyes of band, the cuttage branch is first used into quality hundred
Fraction be 0.05 ~ 0.10% disinfecting solution of potassium permanganate, then with mass percent be 3 ~ 4% peptide pyridimine nucleosides antibiotic water
Agent is soaked 10 ~ 15 minutes, is taken out after the cuttage branch hangs, and cuttage part is immersed in compounding nutrient solution, stands 8 ~ 10 hours,
Take out, the Miao Chizhong that step S1 is prepared is entered in cuttage, after treating cuttage, Miao Chi waterings, seals canopy；
Step S3：Cultivate：The humidity inside the greenhouse is controlled to be not less than 85%, temperature is 18 ~ 25 DEG C, the humidity in the seedling pond
For 70 ~ 75%, after cuttage branch germination, bactericide is periodically sprayed, hardening can be aerated by treating the early spring of Second Year,
Go out garden.
Preferably, the decomposed biogas residue-haydite mixed layer by decomposed biogas residue and haydite with mass ratio 1：2 ~ 4 uniformly mix institute
.
Preferably, the thickness of the decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa be followed successively by 5 ~ 8 centimetres,
12 ~ 18 centimetres, 3 ~ 5 centimetres.
Preferably, the particle diameter of haydite is less than 15 millimeters in the decomposed biogas residue-haydite mixed layer, thin in the fine sand layer
Husky particle diameter is less than 0.35 millimeter, and the particle diameter of light ceramic is less than 8 millimeters in the Lightweight ceramic granulosa.
Preferably, the depth of the cuttage is the 1/3 of the cuttage branch length.
Preferably, the compounding nutrient solution is made up of the composition of following parts by weight：5 ~ 10 parts of pyrolkigneous liquid, granatum extract solution
1 ~ 5 part, 1 ~ 5 part of sponge gourd extract solution, 1 ~ 5 part of lychee exocarp extract solution, 0.005 ~ 0.008 part of heteroauxin.
Preferably, the spacing that the cuttage branch carries out cuttage is 7 ~ 8 centimetres of 7 ~ 8 cm x.
Compared with prior art, its advantage is as follows by the present invention：
The present invention according to Ranunculus asiaticus growth characteristics and nutritional need the features such as, normal cutting propagation propagation method is improved with it is excellent
Change, pass through the comprehensive adjustment of growing environment, nutrition and hormone so that the cuttage survival rate of Ranunculus asiaticus improves, infection pathogen
Probability reduce, lifting is easy, transplanting survival rate improve.
First, on soil horizon, seedling is used as using the combination of decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa
Pond carries out cutting propagation, and Miao Chi makes simply, is easily managed.Decomposed biogas residue-haydite mixed layer is mixed by decomposed biogas residue and haydite
Form, substantial amounts of trace element, organic nutrient substance etc. are contained in decomposed biogas residue, to provide Ranunculus asiaticus sufficient nutrient, simultaneously
Decomposed biogas residue solid content is low, and haydite specific surface area is big, has certain absorption property, can adsorb the part battalion of decomposed biogas residue
Support, improve the solid content of decomposed biogas residue, make it easier to be molded, while efficiency slow release can also be played a part of so that Ranunculus asiaticus skewer
Nutrition obtains uninterrupted in the whole growth course of seedlings plugging；For fine sand as intermediary substrate, ventilation is good, can save water and
Ponding will not be caused, good support is provided for the breeding of cuttage branch, and fine sand layer is loose is not easy to consolidate, cuttage operation
Easily, final-period management is simple.Light ceramic has excellent insulation, performance of keeping humidity, to maintain seedling pond be in suitable temperature with
Humidity has the function that positive；, can by the combination collocation of decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa
Reach accumulation and the slow release effect of nutritional ingredient, providing growth for cuttage branch supports, and can maintains the temperature and humidity in seedling pond
State, it is in cuttage branch and stablizes relatively and in suitable growing environment, promotes quick, the stable growth of cuttage branch.Flower
Buttercup is grown in after cuttage in decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa, and root system is not up to soil
In, during transplanting, lifting operation is easy, and will not injure root system, and this has the function that positive to the survival rate for improving transplanting.
Secondly, the present invention first uses mass percent molten for 0.05 ~ 0.10% potassium permanganate for the cuttage branch of interception
Liquid disinfectant, then soaked 10 ~ 15 minutes with the peptide pyridimine nucleosides antibiotic aqua that mass percent is 3 ~ 4%, it can either reach good
Good disinfection effect, while contribute to the healthy growth of cuttage branch, the survival rate of cuttage branch is significantly improved, and dilute
Multiple is high, does not easily cause pedo relict, bactericidal effect and ageing all apparently higher than the effect of the above two independent roles.Meanwhile
Stop for carrying out 8 ~ 10 hours in the cuttage branch insertion compounding nutrient solution after sterilization so that cuttage branch draws part battalion
Support, while the generation of pre- preventing disease and pest, promote cuttage branch faster, better adapt to cuttage growing environment, Rapid Rooting.Compounding
In nutrient solution, pyrolkigneous liquid can promote cuttage branch to take root, promote root vigor, improve cuttage branch as growth regulator
Growth quality, additionally it is possible to improved soil；Granatum extract solution is extracted by granatum and obtained, and granatum is Punicaceae plant pomegranate
Drying pericarp, granatum extract solution is not only containing the nutritional ingredient such as inorganic mineral, and taking root for cuttage branch can also be improved
Rate, improve the resistance against diseases of cuttage seeding；Sponge gourd extract solution is extracted by sponge gourd and obtained, after sponge gourd extract solution is absorbed by cuttage branch,
Nutriment can be provided for cuttage branch, improve the resistance against diseases of cuttage branch and sprouting, and bloomed after cuttage transplantation of seedlings
Phase is grown, and bulb plumpness is good；Lychee exocarp extract solution is extracted by lychee exocarp and obtained, and lychee exocarp is the outer fruit of sapindaceous plant lichee
Skin, vitamin B1, B2, C, folic acid, citric acid, malic acid, calcium, phosphorus, iron, arginine, tryptophan are contained in lychee exocarp extract solution
Etc. nutritional ingredient, coordinate with granatum extract solution and sponge gourd extract solution, can advantageously promote cuttage branch takes root, and improves cuttage
Branch survival rate, also there is remarkable effect simultaneously for the long-term normal disease-free metaplasia length of cuttage seeding is maintained；Heteroauxin is plant
Thing auxin, appropriate use can promote cuttage branch to take root, germinate in Ranunculus asiaticus cutting propagation；Above-mentioned pyrolkigneous liquid, pomegranate
Skin extract solution, sponge gourd extract solution, lychee exocarp extract solution can significantly improve the rooting rate of Ranunculus asiaticus with heteroauxin compounding, carry
High cuttage survival rate.
Again, the suitable growing environment of cuttage branch is kept during cutting propagation, the morbidity of cuttage branch can be reduced
Probability, the applicant by exploring repeatedly, and by Ranunculus asiaticus cutting propagation, the humid control inside greenhouse is more than 85%, temperature
Control is at 18 ~ 25 DEG C, and Miao Chi humid control is 70 ~ 75%, and cuttage shoot growth is healthy and strong, and adaptability is good.
Embodiment
For a better understanding of the present invention, present disclosure, but this hair are further fairly set out with reference to embodiment
Bright protection content is not limited solely to the following examples.In the following description, give a large amount of concrete details so as to
More thorough understanding of the invention is provided.It will be apparent, however, to one skilled in the art that the present invention can be with
It is carried out without one or more of these details.
Embodiment 1
A kind of cuttage breeding method of Ranunculus asiaticus, comprises the following steps：
Step S1：Build greenhouse, separated in the greenhouse with rib and do some seedling ponds, in the seedling pond by it is bottom up according to
It is secondary uniformly to lay decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa, flat surface；
Step S2：In the dormancy period of Ranunculus asiaticus, the raw then, spray of robust growth is chosen, sturdy position is chosen after cutting, is cut
Cuttage branch is used as into one section of 10 ~ 12 centimeter lengths, every section of at least two eyes of band, the cuttage branch is first used into quality hundred
Fraction is 0.08% disinfecting solution of potassium permanganate, then is soaked with the peptide pyridimine nucleosides antibiotic aqua that mass percent is 3.2%
Bubble 12 minutes, take out after the cuttage branch hangs, cuttage part is immersed in compounding nutrient solution, stands 9 hours, takes out, cuttage
Enter the Miao Chizhong that step S1 is prepared, after treating cuttage, Miao Chi waterings, seal canopy；
Step S3：Cultivate：It is 85% to control the humidity inside the greenhouse, and temperature is 20 DEG C, and the humidity in the seedling pond is 72%, is treated
After the cuttage branch germination, bactericide is periodically sprayed, hardening can be aerated by treating the early spring of Second Year, go out garden.
Decomposed biogas residue-haydite the mixed layer is by decomposed biogas residue and haydite with mass ratio 1：3 uniformly mix gained.
The thickness of the decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa be followed successively by 6 centimetres, 15 centimetres, 4
Centimetre.
The particle diameter of haydite is less than 15 millimeters in the decomposed biogas residue-haydite mixed layer, the grain of fine sand in the fine sand layer
Footpath is less than 0.35 millimeter, and the particle diameter of light ceramic is less than 8 millimeters in the Lightweight ceramic granulosa.
The depth of the cuttage is the 1/3 of the cuttage branch length.
The compounding nutrient solution is made up of the composition of following parts by weight：7 parts of pyrolkigneous liquid, 2 parts of granatum extract solution, sponge gourd carry
Take 3 parts of liquid, 2 parts of lychee exocarp extract solution, 0.006 part of heteroauxin.
The spacing that the cuttage branch carries out cuttage is 7 centimetres of 7 cm x, i.e. 7 centimetres of spacing in the rows, 7 centimetres of line-spacing.
In the present invention, in same greenhouse, Miao Chi quantity can make some ponds according to demand, for example, 1,2,3
Individual, even more more, its quantity is not construed as limiting the invention.Seedling pond is separated using rib described in each two.Miao Chi bottom
Portion is horizontal, and surface is less than rib.
In the present embodiment, the decomposed biogas residue-haydite mixed layer is by decomposed biogas residue and haydite with mass ratio 1：2 ~ 4 is uniformly mixed
Close gained.Decomposed biogas residue is solid-liquid mixture, mixed using haydite, on the one hand can reduce the mobility of decomposed biogas residue,
The part nutrition of decomposed biogas residue can be also adsorbed simultaneously in haydite, reach the effect of sustained release, and decomposed biogas residue makes for a long time
It is not susceptible to lump during, keeps the suitable venting capability in seedling pond.
Meanwhile to reach nutrition supply, water-permeable and air permeable, supporting fixed effect, it is strict to control seedling pond thickness, according to cuttage
Shoot growth situation and character of rooting, the present invention by the thickness of decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa according to
Secondary control is at 5 ~ 8 centimetres, 12 ~ 18 centimetres, 3 ~ 5 centimetres.
In addition, seedling pond of the present invention material therefor need to be controlled in suitable particle size range, make the situations such as seedling pool environment, nutrition
It is homogeneous, to realize the healthy growth of Ranunculus asiaticus cuttage branch, wherein：The particle diameter of haydite is 15 in decomposed biogas residue-haydite mixed layer
Millimeter is following, and preferably 9 ~ 15 millimeters, haydite particle diameter is excessive, and mixing difficulty increase, haydite specific surface area reduces, under absorption property
Drop, all significantly affects nutrition supply, and haydite particle diameter is too small, and permeable breathable reduces, and is also unfavorable for the growth of cuttage branch；Institute
The particle diameter for stating fine sand in fine sand layer is less than 0.35 millimeter, and preferably 0.20 ~ 0.35 millimeter, cuttage branch major part root system is in fine sand
Grown in layer, the excessive then root system of fine sand particle diameter is sparse, is not easy to grow, and particle diameter is too small, influences air-and water-permeable performance；The Lightweight ceramic
The particle diameter of light ceramic is less than 8 millimeters in granulosa, and preferably 3 ~ 8 millimeters, particle diameter is excessive, and cutting density reduces, to the guarantor in seedling pond
Temperature, moisture-keeping function decline.
In the present invention, granatum extract solution, sponge gourd extract solution, lychee exocarp extract solution can refer to following methods and prepare：Respectively
Weigh dry granatum, sponge gourd and lychee exocarp to add in 75% ethanol solution, granatum weight：Volumes of aqueous ethanol is 1g:
15ml, sponge gourd weight：Volumes of aqueous ethanol is 1g:12ml, lychee exocarp weight：Volumes of aqueous ethanol is 1g:18ml, ultrasonic wave bar
15 ~ 18 hours are stood under part, ultrasonic power is 200 ~ 250KW, and frequency is 21 ~ 23KHz, steams ethanol and produces.
Step S2 of the present invention is, it is necessary to cuttage branch is hung after above-mentioned sterilization, and the degree hung is directly to see
The surface for examining cuttage branch is defined without obvious solution residual.
The watering of seedling pond reaches control humidity range to ensure that seedling pond has enough moisture described in step S2 of the present invention.Seedling
Pond watering need to be such that moisture infiltrates through completely in fine sand, and the water surface is just defined with fine sand flush.
Step S3 breeding phases of the present invention, humidity and seedling pond humidity in greenhouse are required for being monitored, regulated and controled in time wet
Degree, when humidity reduces, it is necessary to carry out moisturizing to seedling pond.
Step S3 of the present invention monthly sprays a bactericide, bactericide, which can use, to be had after cuttage branch germination
The medicine of broad-spectrum bactericidal action, such as Bravo, Ah young bird's rhzomorph.
Using the above-mentioned cuttage breeding method of the present embodiment, the survival rate of Ranunculus asiaticus cuttage branch to more than 90%, lifting
The more existing soil lifting of efficiency improves more than 40%, and lifting does not hinder root；Ranunculus asiaticus goes out the survival rate 95% of garden cultivation, more traditional shifting
Plant survival rate and improve more than 12%.
Embodiment 2
A kind of cuttage breeding method of Ranunculus asiaticus, comprises the following steps：
Step S1：Build greenhouse, separated in the greenhouse with rib and do some seedling ponds, in the seedling pond by it is bottom up according to
It is secondary uniformly to lay decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa, flat surface；
Step S2：In the dormancy period of Ranunculus asiaticus, the raw then, spray of robust growth is chosen, sturdy position is chosen after cutting, is cut
Cuttage branch is used as into one section of 10 ~ 12 centimeter lengths, every section of at least two eyes of band, the cuttage branch is first used into quality hundred
Fraction is 0.05% disinfecting solution of potassium permanganate, then is soaked with the peptide pyridimine nucleosides antibiotic aqua that mass percent is 4%
10 minutes, take out after the cuttage branch hangs, cuttage part is immersed in compounding nutrient solution, stands 8 hours, takes out, and cuttage is entered
The Miao Chizhong that step S1 is prepared, after treating cuttage, Miao Chi waterings, seal canopy；
Step S3：Cultivate：It is 90% to control the humidity inside the greenhouse, and temperature is 23 DEG C, and the humidity in the seedling pond is 75%, is treated
After the cuttage branch germination, bactericide is periodically sprayed, hardening can be aerated by treating the early spring of Second Year, go out garden.
Decomposed biogas residue-haydite the mixed layer is by decomposed biogas residue and haydite with mass ratio 1：2 uniformly mix gained.
The thickness of the decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa be followed successively by 5 centimetres, 12 centimetres, 3
Centimetre.
The particle diameter of haydite is less than 15 millimeters in the decomposed biogas residue-haydite mixed layer, the grain of fine sand in the fine sand layer
Footpath is less than 0.35 millimeter, and the particle diameter of light ceramic is less than 8 millimeters in the Lightweight ceramic granulosa.
The depth of the cuttage is the 1/3 of the cuttage branch length.
The compounding nutrient solution is made up of the composition of following parts by weight：5 parts of pyrolkigneous liquid, 1 part of granatum extract solution, sponge gourd carry
Take 1 part of liquid, 1 part of lychee exocarp extract solution, 0.005 part of heteroauxin.
The spacing that the cuttage branch carries out cuttage is 8 centimetres of 8 cm x.
Embodiment 3
A kind of cuttage breeding method of Ranunculus asiaticus, comprises the following steps：
Step S1：Build greenhouse, separated in the greenhouse with rib and do some seedling ponds, in the seedling pond by it is bottom up according to
It is secondary uniformly to lay decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa, flat surface；
Step S2：In the dormancy period of Ranunculus asiaticus, the raw then, spray of robust growth is chosen, sturdy position is chosen after cutting, is cut
Cuttage branch is used as into one section of 10 ~ 12 centimeter lengths, every section of at least two eyes of band, the cuttage branch is first used into quality hundred
Fraction is 0.10% disinfecting solution of potassium permanganate, then is soaked with the peptide pyridimine nucleosides antibiotic aqua that mass percent is 3%
15 minutes, take out after the cuttage branch hangs, cuttage part is immersed in compounding nutrient solution, stands 10 hours, takes out, and cuttage is entered
The Miao Chizhong that step S1 is prepared, after treating cuttage, Miao Chi waterings, seal canopy；
Step S3：Cultivate：It is 88% to control the humidity inside the greenhouse, and temperature is 18 DEG C, and the humidity in the seedling pond is 70%, is treated
After the cuttage branch germination, bactericide is periodically sprayed, hardening can be aerated by treating the early spring of Second Year, go out garden.
Decomposed biogas residue-haydite the mixed layer is by decomposed biogas residue and haydite with mass ratio 1：4 uniformly mix gained.
The thickness of the decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa be followed successively by 8 centimetres, 18 centimetres, 5
Centimetre.
The particle diameter of haydite is less than 15 millimeters in the decomposed biogas residue-haydite mixed layer, the grain of fine sand in the fine sand layer
Footpath is less than 0.35 millimeter, and the particle diameter of light ceramic is less than 8 millimeters in the Lightweight ceramic granulosa.
The depth of the cuttage is the 1/3 of the cuttage branch length.
The compounding nutrient solution is made up of the composition of following parts by weight：10 parts of pyrolkigneous liquid, 5 parts of granatum extract solution, sponge gourd
5 parts of extract solution, 5 parts of lychee exocarp extract solution, 0.008 part of heteroauxin.
The spacing that the cuttage branch carries out cuttage is 8 centimetres of 7 cm x, i.e. 7 centimetres of spacing in the rows, 8 centimetres of line-spacing.
Embodiment 4
A kind of cuttage breeding method of Ranunculus asiaticus, comprises the following steps：
Step S1：Build greenhouse, separated in the greenhouse with rib and do some seedling ponds, in the seedling pond by it is bottom up according to
It is secondary uniformly to lay decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa, flat surface；
Step S2：In the dormancy period of Ranunculus asiaticus, the raw then, spray of robust growth is chosen, sturdy position is chosen after cutting, is cut
Cuttage branch is used as into one section of 10 ~ 12 centimeter lengths, every section of at least two eyes of band, the cuttage branch is first used into quality hundred
Fraction is 0.08% disinfecting solution of potassium permanganate, then is soaked with the peptide pyridimine nucleosides antibiotic aqua that mass percent is 3.5%
Bubble 13 minutes, take out after the cuttage branch hangs, cuttage part is immersed in compounding nutrient solution, stands 8.5 hours, takes out, skewer
The Miao Chizhong that step S1 is prepared is injected, after treating cuttage, Miao Chi waterings, seals canopy；
Step S3：Cultivate：It is 85% to control the humidity inside the greenhouse, and temperature is 25 DEG C, and the humidity in the seedling pond is 73%, is treated
After the cuttage branch germination, bactericide is periodically sprayed, hardening can be aerated by treating the early spring of Second Year, go out garden.
Decomposed biogas residue-haydite the mixed layer is by decomposed biogas residue and haydite with mass ratio 1：3 uniformly mix gained.
The thickness of the decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa be followed successively by 7 centimetres, 16 centimetres, 4
Centimetre.
The particle diameter of haydite is less than 15 millimeters in the decomposed biogas residue-haydite mixed layer, the grain of fine sand in the fine sand layer
Footpath is less than 0.35 millimeter, and the particle diameter of light ceramic is less than 8 millimeters in the Lightweight ceramic granulosa.
The depth of the cuttage is the 1/3 of the cuttage branch length.
The compounding nutrient solution is made up of the composition of following parts by weight：8 parts of pyrolkigneous liquid, 4 parts of granatum extract solution, sponge gourd carry
Take 4 parts of liquid, 3 parts of lychee exocarp extract solution, 0.007 part of heteroauxin.
The spacing that the cuttage branch carries out cuttage is 7 centimetres of 8 cm x.
Embodiment 5
The cuttage breeding method of Ranunculus asiaticus described by the present embodiment, it is substantially the same manner as Example 1, except that：
In step S2, the compounding nutrient solution is made up of the composition of following parts by weight：5 parts of pyrolkigneous liquid, 2 parts of granatum extract solution,
1 part of sponge gourd extract solution, 2 parts of lychee exocarp extract solution, 0.005 part of heteroauxin.
Embodiment 6
The cuttage breeding method of Ranunculus asiaticus described by the present embodiment, it is substantially the same manner as Example 1, except that：
In step S2, the compounding nutrient solution is made up of the composition of following parts by weight：7 parts of pyrolkigneous liquid, 3 parts of granatum extract solution,
2 parts of sponge gourd extract solution, 1 part of lychee exocarp extract solution, 0.005 part of heteroauxin.
Embodiment 7
The cuttage breeding method of Ranunculus asiaticus described by the present embodiment, it is substantially the same manner as Example 1, except that：
In step S2, the compounding nutrient solution is made up of the composition of following parts by weight：9 parts of pyrolkigneous liquid, 1 part of granatum extract solution,
3 parts of sponge gourd extract solution, 4 parts of lychee exocarp extract solution, 0.006 part of heteroauxin.
Embodiment 8
The cuttage breeding method of Ranunculus asiaticus described by the present embodiment, it is substantially the same manner as Example 1, except that：
In step S2, the compounding nutrient solution is made up of the composition of following parts by weight：10 parts of pyrolkigneous liquid, 4 parts of granatum extract solution,
5 parts of sponge gourd extract solution, 4 parts of lychee exocarp extract solution, 0.007 part of heteroauxin.
Comparative example 1
A kind of cuttage breeding method of Ranunculus asiaticus, it is substantially the same manner as Example 1, except that：
Step S1：Build greenhouse, separated in the greenhouse with rib and do some seedling ponds, in the seedling pond by it is bottom up according to
It is secondary uniformly to lay decomposed biogas residue layer, fine sand layer and Lightweight ceramic granulosa, flat surface；
Wherein, the thickness of the decomposed biogas residue layer, fine sand layer and Lightweight ceramic granulosa is followed successively by 6 centimetres, 15 centimetres, 4 centimetres；It is described
The particle diameter of fine sand is less than 0.35 millimeter in fine sand layer, and the particle diameter of light ceramic is less than 8 millimeters in the Lightweight ceramic granulosa.
Comparative example 2
A kind of cuttage breeding method of Ranunculus asiaticus, it is substantially the same manner as Example 1, except that：
Step S1：Build greenhouse, separated in the greenhouse with rib and do some seedling ponds, in the seedling pond by it is bottom up according to
It is secondary uniformly to lay decomposed biogas residue layer and fine sand layer, flat surface；
Wherein, the decomposed biogas residue layer, the thickness of fine sand layer are followed successively by 6 centimetres, 15 centimetres；The particle diameter of fine sand in the fine sand layer
For less than 0.35 millimeter.
Comparative example 3
A kind of cuttage breeding method of Ranunculus asiaticus, it is substantially the same manner as Example 1, except that：
Step S2：In the dormancy period of Ranunculus asiaticus, the raw then, spray of robust growth is chosen, sturdy position is chosen after cutting, is cut
Cuttage branch is used as into one section of 10 ~ 12 centimeter lengths, every section of at least two eyes of band, the cuttage branch is used into quality percentage
Number is 0.10% disinfecting solution of potassium permanganate, is taken out after the cuttage branch hangs, and cuttage part is immersed in compounding nutrient solution,
9 hours are stood, is taken out, the Miao Chizhong that step S1 is prepared is entered in cuttage, after treating cuttage, Miao Chi waterings, seals canopy.
Wherein, the composition for compounding nutrient solution is same as Example 1.
Comparative example 4
A kind of cuttage breeding method of Ranunculus asiaticus, it is substantially the same manner as Example 1, except that：
Step S2：In the dormancy period of Ranunculus asiaticus, the raw then, spray of robust growth is chosen, sturdy position is chosen after cutting, is cut
Cuttage branch is used as into one section of 10 ~ 12 centimeter lengths, every section of at least two eyes of band, the cuttage branch is first used into quality hundred
Fraction is 0.08% disinfecting solution of potassium permanganate, then is soaked with the peptide pyridimine nucleosides antibiotic aqua that mass percent is 3.2%
Bubble 12 minutes, take out after the cuttage branch hangs, the Miao Chizhong that step S1 is prepared, after treating cuttage, Miao Chi are entered in cuttage
Watering, seal canopy.
Comparative example 5
A kind of cuttage breeding method of Ranunculus asiaticus, it is substantially the same manner as Example 1, except that：
In step S2, the compounding nutrient solution is made up of the composition of following parts by weight：7 parts of pyrolkigneous liquid, 0.006 part of heteroauxin.
Comparative example 6
A kind of cuttage breeding method of Ranunculus asiaticus, it is substantially the same manner as Example 1, except that：
In step S2, the compounding nutrient solution is made up of the composition of following parts by weight：7 parts of pyrolkigneous liquid, 2 parts of granatum extract solution,
3 parts of sponge gourd extract solution, 0.006 part of heteroauxin.
Comparative example 7
A kind of cuttage breeding method of Ranunculus asiaticus, it is substantially the same manner as Example 1, except that：
In step S2, the compounding nutrient solution is made up of the composition of following parts by weight：7 parts of pyrolkigneous liquid, 2 parts of lychee exocarp extract solution,
0.006 part of heteroauxin.
Effect assessment
Evaluation index：Cuttage complexity, cuttage survival rate, lifting hinder root degree, bulb quality, transplanting survival rate.
The basis for estimation of above-mentioned judge index is as follows in the present invention：
Cuttage complexity：The probability of cuttage in place, >=95% is easy, and 95 ~ 85% is are easier to, 85 ~ 75% be it is more difficult,
Less than 75% is difficult；
Cuttage survival rate：The cuttage branch quantity survived after cutting propagation and the cuttage branch ratio of number of initial cuttage, with % tables
Show；
Lifting hinders root degree：It is defined by 100 seedlings, in the case of normal operation, hinders within 2 plants of radical amount not hinder root, hinder root
2 ~ 10 plants of quantity is slightly to hinder root；It is to be easier to hinder root to hinder 10 ~ 20 plants of root；Hinder root more than 20 easily to hinder root.
Bulb quality：Detection method and decision principle are carried out by GB2772；Girth diameter：With the week of girth diameter dimensional measurement bulb
Long, reading is accurate to 1cm；Plumpness：Carried out by the chapters of GB 2772-1999 the 7th；Pest and disease damage：Enter by the chapters of GB 2772-1999 the 8th
OK；Sampling：Carried out by the chapters of GB 2772-1999 the 3rd；Grade：According to GB/T18247.6-2000《Main flowers product hierarchy
6th part：Flowers bulb》The grade criterion of middle bulb is judged.
Transplanting survival rate：The quantity of Ranunculus asiaticus transplant survival and the ratio of number initially transplanted, are represented with %.
Following table is the evaluation index result of the embodiment of the present invention 1 ~ 3 and comparative example 1 ~ 7
。
As can be seen from the above table, using the present invention carry out Ranunculus asiaticus cutting propagation bring notable results, cuttage is easy, cuttage into
Motility rate is higher than 90%, and lifting is easy, does not hinder root, and bulb quality one-level, transplanting survival rate is higher than 95%.1 reality more of the present invention of comparative example
Example 1 to be applied, decomposed biogas residue is laid in seedling pond and is not mixed using haydite, experimental result shows that cuttage survival rate drops to 78.3%,
Lifting is easier to hinder root, and bulb girth diameter is smaller, and it is more single as bottom laying to illustrate that decomposed biogas residue and haydite mix by the present invention
The one decomposed biogas residue effect of laying is more notable, enables in particular to improve cuttage survival rate.Comparative example 2 is compared with the embodiment of the present invention 1, in seedling pond
Lay decomposed biogas residue not mix using haydite, and do not lay light ceramic on upper strata, experimental result is shown, cuttage difficulty increases
Greatly, cuttage survival rate drops to 71.6%, easily hinders root, and bulb girth diameter is smaller, and plumpness is good, further illustrates step of the present invention
The laying of seedling pond is reasonable in S1, can preferably meet cuttage and the nutritional need of Ranunculus asiaticus, and make it that growth of cuttage seedlings is more healthy and stronger,
Finished product bulb better quality.Comparative example 3 is not soaked using peptide pyridimine nucleosides antibiotic aqua to cuttage branch, is as a result shown
Show, finished product bulb Quality Down, bulb disease rate is 8.3%, illustrates the use of mentioned component and can improve the long-term anti-of cuttage seeding
Sick ability.Comparative example 4 is not soaked using compounding nutrient solution to cuttage branch, is as a result shown, cuttage survival rate is reduced to
53.6%, cuttage seeding is more weak, lifting is easier to hinder root, and bulb is of poor quality, disease rate 17.1%, and insect pest rate is 7.6%, overall effect
Fruit is remarkably decreased, and illustrates the use of present invention compounding nutrient solution and can significantly improve growth and the fertility of cuttage branch, and
The long-term diseases and insect pests resistance of Ranunculus asiaticus can be improved.Comparative example 5 ~ 7 changes the composition of compounding nutrient solution, as a result shows, more right
Though ratio 4 makes moderate progress in various degree, general effect is still poor compared with embodiment 1, illustrates that compositional liquor of the present invention assembles rationally,
Synergistic can be played, the common adaptive capacity to environment and diseases and insect pests resistance for improving Ranunculus asiaticus, significant effect.
The above results and analysis shows, the cuttage breeding method of Ranunculus asiaticus of the present invention is good to Ranunculus asiaticus adaptability, cuttage into
Motility rate is high, and cuttage is easily and lifting does not hinder root, and Ranunculus asiaticus transplanting survival rate is high, and finished product bulb quality is good, and diseases and insect pests resistance is strong.
Although the foregoing describing the embodiment of the present invention, those familiar with the art should manage
Solution, the specific embodiment described by us are merely exemplary, rather than for the restriction to the scope of the present invention, are familiar with this
The equivalent modification and change that the technical staff in field is made in the spirit according to the present invention, should all cover the present invention's
In scope of the claimed protection.
Claims (7)
- A kind of 1. cuttage breeding method of Ranunculus asiaticus, it is characterised in that：Comprise the following steps：Step S1：Build greenhouse, separated in the greenhouse with rib and do some seedling ponds, in the seedling pond by it is bottom up according to It is secondary uniformly to lay decomposed biogas residue-haydite mixed layer, fine sand layer and Lightweight ceramic granulosa, flat surface；Step S2：In the dormancy period of Ranunculus asiaticus, the raw then, spray of robust growth is chosen, sturdy position is chosen after cutting, is cut Cuttage branch is used as into one section of 10 ~ 12 centimeter lengths, every section of at least two eyes of band, the cuttage branch is first used into quality hundred Fraction be 0.05 ~ 0.10% disinfecting solution of potassium permanganate, then with mass percent be 3 ~ 4% peptide pyridimine nucleosides antibiotic water Agent is soaked 10 ~ 15 minutes, is taken out after the cuttage branch hangs, and cuttage part is immersed in compounding nutrient solution, stands 8 ~ 10 hours, Take out, the Miao Chizhong that step S1 is prepared is entered in cuttage, after treating cuttage, Miao Chi waterings, seals canopy；Step S3：Cultivate：The humidity inside the greenhouse is controlled to be not less than 85%, temperature is 18 ~ 25 DEG C, the humidity in the seedling pond For 70 ~ 75%, after cuttage branch germination, bactericide is periodically sprayed, hardening can be aerated by treating the early spring of Second Year, Go out garden.
- 2. the cuttage breeding method of Ranunculus asiaticus as claimed in claim 1, it is characterised in that：The decomposed biogas residue-haydite mixing Layer is by decomposed biogas residue and haydite with mass ratio 1：2 ~ 4 uniformly mix gained.
- 3. the cuttage breeding method of Ranunculus asiaticus as claimed in claim 2, it is characterised in that：The decomposed biogas residue-haydite mixing The thickness of layer, fine sand layer and Lightweight ceramic granulosa is followed successively by 5 ~ 8 centimetres, 12 ~ 18 centimetres, 3 ~ 5 centimetres.
- 4. the cuttage breeding method of Ranunculus asiaticus as claimed in claim 3, it is characterised in that：The decomposed biogas residue-haydite mixing The particle diameter of haydite is less than 15 millimeters in layer, and the particle diameter of fine sand is less than 0.35 millimeter in the fine sand layer, the light ceramic The particle diameter of light ceramic is less than 8 millimeters in layer.
- 5. the cuttage breeding method of Ranunculus asiaticus as claimed in claim 1, it is characterised in that：The depth of the cuttage is the skewer Insert the 1/3 of branch length.
- 6. the cuttage breeding method of Ranunculus asiaticus as claimed in claim 1, it is characterised in that：The compounding nutrient solution by weighing as follows The composition of amount part is made：5 ~ 10 parts of pyrolkigneous liquid, 1 ~ 5 part of granatum extract solution, 1 ~ 5 part of sponge gourd extract solution, lychee exocarp extract solution 1 ~ 5 Part, 0.005 ~ 0.008 part of heteroauxin.
- 7. the cuttage breeding method of Ranunculus asiaticus as claimed in claim 1, it is characterised in that：The cuttage branch carries out cuttage Spacing is 7 ~ 8 centimetres of 7 ~ 8 cm x.
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