CN103430656B - Seedling culture method capable of relieving pear seeds from dormancy - Google Patents
Seedling culture method capable of relieving pear seeds from dormancy Download PDFInfo
- Publication number
- CN103430656B CN103430656B CN201310342164.8A CN201310342164A CN103430656B CN 103430656 B CN103430656 B CN 103430656B CN 201310342164 A CN201310342164 A CN 201310342164A CN 103430656 B CN103430656 B CN 103430656B
- Authority
- CN
- China
- Prior art keywords
- seed
- pears
- seeds
- dormancy
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y02P60/216—
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the field of plant biotechnology, and particularly relates to a seedling culture method capable of relieving pear seeds from dormancy, which comprises steps of seed selection, immersion, mixture preparation, tray seedling culture and culture management. According to the method, the procedures of pear seed dormancy relief and tray seedling culture are combined together, the seeds are relieved from dormancy and impelled to sprout due to the temperature and humidity environment of a seedling culture tray, the examination for complete relief of seed dormancy is avoided, the seeds can automatically turn into the tray seedling culture stage after being relieved from dormancy, the seed dormancy can be broken to the maximum extent, the emergence rate is also improved, and the germination rate achieves more than 96%. According to the invention, the pears are stacked under the condition of 25 DEG C for 5 to 7 days, and then the seeds are taken out from the pears, so that the after-ripening effect of the seeds can be achieved; after the after-ripening effect, the germination rate of the seeds is greatly improved, and the relief of seed dormancy is facilitated. The pear seeds are hard seeds with poor water permeability. According to the method, concentrated sulfuric acid is adopted for soaking the seeds, so that the barrier of the seed coat is broken and the germination of the seeds is greatly facilitated.
Description
Technical field
The invention belongs to plant biotechnology field, be specifically related to the seed dormant seedling-cultivating method of a kind of Rapid reversal pears.
Background technology
Pears are rose family Pyrus L, perennial deciduous fruit tree, arbor, and leaf is avette, spend how white, fruit succulence, edible.The good reputation that pears have " ancestors of all kinds of fruits ", because of its fresh and tender succulence, sour and sweet palatability, so there is again the title of " natural mineral water ", in world's fruit market, is called as with apple and orange " three large fruits seize by force ".Cultivation mainly contains Ussurian pear, white pear, sand pear, foreign pears four systems.Pear cultivation is with a long history, and kind is wide in variety, and strong adaptability is easily given stable high yields irrespective of drought or water logging, and deeply welcomes by the masses.
Seed dormancy is a normal physiological phenomena of plant growth and development process, is that plant is to environmental condition and the biological adaptability of seasonal variety.Pears seed ubiquity dormancy.Seed coat obstacle, kind embryo dormancy and kind embryo after-ripening etc. cause the seed dormant main cause of pears.In the practices of breeding, it just can need be made to complete the after-ripening of embryo by after real for the allocarpy certain hour of storage under cryogenic.Even if but like this, still have that hybrid seed emergence rate is low, the problem such as irregular of emerging.Current, obtaining the improved Varieties pears with characteristics such as high yield, disease and insect resistance, anti-extraneous bad conditions by selection cross constantly increases.But in crossbreeding, if seed dormancy can not be removed in time, often there is the situation that germination rate is low.
The general method adopting sand to hide removes seed dormancy at present, but this method easily causes seed sprouting to prolong
Late, seed is easily mouldy and in the process of sand Tibetan.The measures such as the employing also had is peeled off, peeling, complexity is taken a lot of work, and has had a strong impact on breeding work efficiency.Therefore the seed dormant processing method of better releasing is sought, to shorten pears seed germination required time and raising
Germination rate, is of great significance pear cultivation nursery and breeding tool.
Summary of the invention
The object of this invention is to provide the seed dormant seedling-cultivating method of a kind of Rapid reversal pears, not only required time short but also
Germination rate is high, greatly facilitates the research of pear cultivation nursery and breeding.
The technical solution used in the present invention is, the seed dormant seedling-cultivating method of a kind of Rapid reversal pears, comprises the following steps:
Step one: choose maturation, pears without insect pest, pears being placed on temperature is stack 5 ~ 7 days under the condition of 25 DEG C, then from pears, takes out seed;
Step 2: with the pears seed in concentrated sulfuric acid soaking step one 1 ~ 2 minute, to proceed to mass concentration be afterwards 500 ~
Take out after soaking 12 ~ 24h in the gibberellin of 800mg/L, for subsequent use;
Step 3: the pears seed of step 2 process is mixed with perlite and the wet husky volume ratio by 1: 1: 3 ~ 5, form mixture I, saccharomycete and human excrement is added in mixture I, fully mix rear formation mixture II, saccharomycetic addition is 1.0 ~ 2.5 ﹪ of mixture I quality, the addition of human excrement is 4.5 ~ 6.5 ﹪ of mixture I quality, and in described wet sand, moisture weight is 50 ~ 60%;
Step 4: choose nursery cave and coil, cave tray bottom paving spills the wet sand that thickness is 2 ~ 3cm, wet sand repaves and spills the mixture II that thickness is 3 ~ 4cm, cladding thickness is the wet sand of 1 ~ 2cm more afterwards, afterwards cave dish is moved to seedbed, water once permeable to the matrix in the dish of cave, in described seedbed, the depth of water remains on 1 ~ 2cm;
Step 5: seedbed being placed in temperature is cultivate under the condition of 7 ~ 13 DEG C, every day light application time 8 ~ 12 hours, intensity of illumination 80 ~ 120lx, seed starts to sprout in rear transplanting to brooder and manages.
Described wet grains of sand degree is 60 ~ 100 orders.
The invention has the beneficial effects as follows, the present invention will break seed dormancy and point disk seedling growing combines, and utilizes nursery
Temp. and humidity environment in dish impels seed breaking dormancy and sprouting, and need not check whether and break seed dormancy completely, seed is broken
Automatically enter the point disk seedling growing stage after dormancy, can break seed dormancy to greatest extent, improve emergence rate simultaneously, germination rate reaches
More than 96%.It is stack 5 ~ 7 days under the condition of 25 DEG C that pears are placed on temperature by the present invention, from pears, then takes out the effect that seed serves seed afterripening, by afterripening, substantially increase percentage of seedgermination, facilitate seed dormant releasing.Pear tree seed is strong property seed, bad hydraulic permeability, and having to pass through suitably process raising seed coat permeability could sprout.The present invention adopts concentrated sulfuric acid immersion to break seed coat obstacle, greatly facilitates the sprouting of seed, proceeds in gibberellin afterwards and soak, shorten and remove the seed dormancy time used.The present invention by the cave dish being loaded with pears seed is put on seedbed and cultivated, well can keep kind of subenvironment
Humidity, had both avoided humidity too low, was unfavorable for breaking dormancy and seed germination, it also avoid that humidity is too high and the seed that causes rotten
Rotten.The present invention is added with the gas permeability that perlite can improve matrix in the dish of cave in pears seed and wet sand, and the present invention is also coiled in cave
Be added with saccharomycete in interior matrix, saccharomycetes to make fermentation produces the matrix that bubble can loosen in the dish of cave, promotes that the breathing of seed is done
With, it also avoid the mold of seed simultaneously, improve germination rate.The sprouting stage of pears seed needs to consume large amount of organic matter, single
The organic substance of pure dependence in health can not satisfy the demands completely, is added with human excrement, treats in the matrix of the present invention in the dish of cave
After pears seed germination grows young root, organic matter can be provided for pears seed, thus promote the Rapid development of pears seedling.
Embodiment
Below embodiments of the invention are elaborated: the present embodiment carries out under premised on technical solution of the present invention
Implement, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following enforcement
Example.
The seed dormant seedling-cultivating method of a kind of Rapid reversal pears, comprises the following steps:
Step one: choose maturation, pears without insect pest, pears being placed on temperature is stack 5 ~ 7 days under the condition of 25 DEG C, then from
Seed is taken out in pears;
Step 2: with the pears seed in concentrated sulfuric acid soaking step one 1 ~ 2 minute, to proceed to mass concentration be afterwards 500 ~
Take out after soaking 12 ~ 24h in the gibberellin of 800mg/L, for subsequent use;
Step 3: the pears seed of step 2 process is mixed with perlite and the wet husky volume ratio by 1: 1: 3 ~ 5, is formed
Mixture I, adds saccharomycete and human excrement in mixture I, fully mixes rear formation mixture II, saccharomyceticly adds
Amount is 1.0 ~ 2.5 ﹪ of mixture I quality, and the addition of human excrement is 4.5 ~ 6.5 ﹪ of mixture I quality, described wet
In sand, moisture weight is 50 ~ 60%;
Step 4: choose nursery cave and coil, tray bottom paving in cave spills the wet sand that thickness is 2 ~ 3cm, wet sand repaves spill thickness and be
The mixture II of 3 ~ 4cm, cladding thickness is the wet sand of 1 ~ 2cm more afterwards, afterwards cave dish is moved to seedbed, and water once permeable to the matrix in the dish of cave, in described seedbed, the depth of water remains on 1 ~ 2cm;
Step 5: seedbed being placed in temperature is cultivate under the condition of 7 ~ 13 DEG C, every day light application time 8 ~ 12 hours, illumination
Intensity 80 ~ 120lx, seed starts to sprout in rear transplanting to brooder and manages.
Described wet grains of sand degree is 60 ~ 100 orders.
Embodiment 1
The seed dormant seedling-cultivating method of a kind of Rapid reversal pears, comprises the following steps:
Step one: choose maturation, pears without insect pest, pears being placed on temperature is stack 5 days under the condition of 25 DEG C, then from pears
Take out seed;
Step 2: the pears seed in use concentrated sulfuric acid soaking step one 1 minute, proceeding to mass concentration is afterwards take out after soaking 12h in the gibberellin of 500mg/L, for subsequent use;
Step 3: the pears seed of step 2 process is mixed with perlite and the wet husky volume ratio by 1: 1: 3, form mixture I, saccharomycete and human excrement is added in mixture I, fully mix rear formation mixture II, saccharomycetic addition is 1.0 ﹪ of mixture I quality, the addition of human excrement is 4.5 ﹪ of mixture I quality, and in described wet sand, moisture weight is 50%;
Step 4: choose nursery cave and coil, cave tray bottom paving spills the wet sand that thickness is 2cm, wet sand repaves and spills the mixture II that thickness is 3cm, cladding thickness is the wet sand of 1cm more afterwards, afterwards cave dish is moved to seedbed, water once permeable to the matrix in the dish of cave, in described seedbed, the depth of water remains on 1cm;
Step 5: seedbed being placed in temperature is cultivate under the condition of 7 DEG C, every day light application time 8 hours, intensity of illumination 80lx, seed starts to sprout in rear transplanting to brooder and manages.
Described wet grains of sand degree is 60 ~ 100 orders.
Embodiment 2
The seed dormant seedling-cultivating method of a kind of Rapid reversal pears, comprises the following steps:
Step one: choose maturation, pears without insect pest, pears being placed on temperature is stack 7 days under the condition of 25 DEG C, then from pears
Take out seed;
Step 2: the pears seed in use concentrated sulfuric acid soaking step one 2 minutes, proceeding to mass concentration is afterwards the red mould of 800mg/L
Take out after soaking 24h in element, for subsequent use;
Step 3: the pears seed of step 2 process is mixed with perlite and the wet husky volume ratio by 1: 1: 5, is formed mixed
Compound I, adds saccharomycete and human excrement in mixture I, fully mixes rear formation mixture II, saccharomycetic addition
For 2.5 ﹪ of mixture I quality, the addition of human excrement is 6.5 ﹪ of mixture I quality, and in described wet sand, moisture weight is
60% ；
Step 4: choose nursery cave and coil, tray bottom paving in cave spills the wet sand that thickness is 3cm, and wet sand repaving and spills thickness is 4cm
Mixture II, cladding thickness is the wet sand of 2cm more afterwards, afterwards by cave dish move to seedbed, to cave dish in matrix water once
Permeable, in described seedbed, the depth of water remains on 2cm;
Step 5: seedbed being placed in temperature is cultivate under the condition of 13 DEG C, every day light application time 12 hours, intensity of illumination
120lx, seed starts to sprout in rear transplanting to brooder and manages.
Described wet grains of sand degree is 60 ~ 100 orders.
Claims (1)
1. the seed dormant seedling-cultivating method of Rapid reversal pears, is characterized in that, comprise the following steps:
Step one: choose maturation, pears without insect pest, pears being placed on temperature is stack 5 ~ 7 days under the condition of 25 DEG C, then from pears, takes out seed;
Step 2: the pears seed in use concentrated sulfuric acid soaking step one 1 ~ 2 minute, proceeding to mass concentration is afterwards take out after soaking 12 ~ 24h in the gibberellin of 500 ~ 800mg/L, for subsequent use;
Step 3: the pears seed of step 2 process is mixed with perlite and the wet husky volume ratio by 1: 1: 3 ~ 5, form mixture I, saccharomycete and human excrement is added in mixture I, fully mix rear formation mixture II, saccharomycetic addition is 1.0 ~ 2.5 ﹪ of mixture I quality, the addition of human excrement is 4.5 ~ 6.5 ﹪ of mixture I quality, and in described wet sand, moisture weight is 50 ~ 60%;
Step 4: choose nursery cave and coil, cave tray bottom paving spills the wet sand that thickness is 2 ~ 3cm, wet sand repaves and spills the mixture II that thickness is 3 ~ 4cm, cladding thickness is the wet sand of 1 ~ 2cm more afterwards, afterwards cave dish is moved to seedbed, water once permeable to the matrix in the dish of cave, in described seedbed, the depth of water remains on 1 ~ 2cm;
Step 5: seedbed being placed in temperature is cultivate under the condition of 7 ~ 13 DEG C, every day light application time 8 ~ 12 hours, intensity of illumination 80 ~ 120lx, seed starts to sprout in rear transplanting to brooder and manages; Described wet grains of sand degree is 60 ~ 100 orders.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310342164.8A CN103430656B (en) | 2013-08-08 | 2013-08-08 | Seedling culture method capable of relieving pear seeds from dormancy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310342164.8A CN103430656B (en) | 2013-08-08 | 2013-08-08 | Seedling culture method capable of relieving pear seeds from dormancy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103430656A CN103430656A (en) | 2013-12-11 |
| CN103430656B true CN103430656B (en) | 2015-06-17 |
Family
ID=49684457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310342164.8A Expired - Fee Related CN103430656B (en) | 2013-08-08 | 2013-08-08 | Seedling culture method capable of relieving pear seeds from dormancy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103430656B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067726B (en) * | 2014-07-11 | 2016-06-01 | 任晓萍 | The seed seedling-raising method of a kind of birch-leaf pear |
| CN104938325A (en) * | 2015-06-23 | 2015-09-30 | 安徽省农业科学院园艺研究所 | Strawberry hybridization and seed seedling method |
| CN105123026A (en) * | 2015-09-25 | 2015-12-09 | 镇江瑞繁农艺有限公司 | Treatment method of seedless watermelon seeds |
| CN105379468B (en) * | 2015-12-03 | 2018-05-25 | 青岛百瑞吉生物工程有限公司 | A kind of method for treating seeds for releasing Fruit Tree Seeds dormancy |
| CN107079627A (en) * | 2017-03-17 | 2017-08-22 | 界首市伟盛家庭农场 | A kind of Dangshan pear method for treating seeds |
| CN106987592B (en) * | 2017-04-07 | 2020-05-19 | 扬州大学 | Method for inhibiting expression of gibberellin synthetic gene of pear seed |
| CN107873164A (en) * | 2017-09-19 | 2018-04-06 | 蚌埠市众星蔬果科技专业合作社联合社 | A kind of breeding method of selenium-rich pear tree |
| CN107750511A (en) * | 2017-09-19 | 2018-03-06 | 蚌埠市众星蔬果科技专业合作社联合社 | A kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition |
| CN107646237A (en) * | 2017-10-26 | 2018-02-02 | 铜仁市万山区吊井文祺生态养殖有限公司 | A kind of method for culturing seedlings for breaking pears seed dormancy |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004049778A1 (en) * | 2002-12-03 | 2004-06-17 | Monsanto Technology, Llc | Method of protecting seeds treated with a phytotoxic agent |
| CN1806525A (en) * | 2006-01-08 | 2006-07-26 | 陈运章 | Method for introducing, domesticating and cultivating superior high-yield seedling of mountain area precious wild thorn |
| CN1989799A (en) * | 2005-12-31 | 2007-07-04 | 刘春山 | Sock growing nursery method for dwarf pear tree |
| CN101507383A (en) * | 2009-04-03 | 2009-08-19 | 湖北省农业科学院果树茶叶研究所 | Germinating breeding method of Chinese pear cross-breed |
| CN101861818A (en) * | 2010-06-21 | 2010-10-20 | 吴江东之田木农业生态园 | Method for quickly breaking birchleaf pear rootstock seed dormancy by using sodium nitroprusside |
-
2013
- 2013-08-08 CN CN201310342164.8A patent/CN103430656B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004049778A1 (en) * | 2002-12-03 | 2004-06-17 | Monsanto Technology, Llc | Method of protecting seeds treated with a phytotoxic agent |
| CN1989799A (en) * | 2005-12-31 | 2007-07-04 | 刘春山 | Sock growing nursery method for dwarf pear tree |
| CN1806525A (en) * | 2006-01-08 | 2006-07-26 | 陈运章 | Method for introducing, domesticating and cultivating superior high-yield seedling of mountain area precious wild thorn |
| CN101507383A (en) * | 2009-04-03 | 2009-08-19 | 湖北省农业科学院果树茶叶研究所 | Germinating breeding method of Chinese pear cross-breed |
| CN101861818A (en) * | 2010-06-21 | 2010-10-20 | 吴江东之田木农业生态园 | Method for quickly breaking birchleaf pear rootstock seed dormancy by using sodium nitroprusside |
Non-Patent Citations (2)
| Title |
|---|
| 化学药剂及温光条件对促进杜梨种子萌发的影响;吴翠云 等;《种子》;20090125;第28卷(第1期);第109页右栏 * |
| 早熟砂梨种子萌发的影响因素分析;王月志 等;《浙江农业学报》;20120811(第8期);第1130页右栏至第1132页左栏 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103430656A (en) | 2013-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103430656B (en) | Seedling culture method capable of relieving pear seeds from dormancy | |
| CN103299812B (en) | Apocarya hardwood cutting seedling raising method | |
| CN101379918B (en) | Stem cottage propagation tree-reconstruction method | |
| CN104737670A (en) | Seedling growing method for oil expression peony seeds | |
| CN102783349B (en) | Method for improving breeding quality of dalbergia odorifera cuttings | |
| CN103210848A (en) | Broccoli lateral branch cultivating plant and hybrid seed production method | |
| CN103609295A (en) | Method for large-scale cultivation of jatropha curcas seedlings | |
| CN105165512A (en) | Crown inclination controlling four-season cutting rapid propagation method for alnus formosana | |
| CN103053395A (en) | Hole tray substrate for fast cultivation of bonsai semperflorens and use method | |
| CN101273692B (en) | Method for cutting, breeding and seedling of Wild Vitis quinquangularis in Guangxi Province | |
| CN103636374B (en) | The light filling seedling-cultivating method of lijiang spruce container seedling and application | |
| CN103039330B (en) | Formula of tray substrate for fast rooting of jade color osmanthus cuttings and application thereof | |
| CN103460940A (en) | Machilus pauhoi full-exposure cuttage fast propagation method | |
| CN108307911B (en) | Root cutting seedling raising method for fish wood | |
| CN105493906A (en) | Orange seedling grafting method | |
| CN105123253B (en) | A kind of method for culturing seedlings of paris polyphylla | |
| CN102668988A (en) | Fast seedling breeding method for tissue cultivation of callicarpa bodinieri and method for transplanting rooting seedlings of callicarpa bodinieri | |
| CN104737761B (en) | Cutting propagation method of sinojackia xylocarpa | |
| CN101595825A (en) | The fast breeding method of sandalwood seedling | |
| CN105461383A (en) | Container nursery light substrate for eucalyptus and preparation method for container nursery light substrate | |
| CN107306733A (en) | A kind of method of blueberry cuttage and seedling culture | |
| CN106069535A (en) | A kind of method improving seed of Fructus Vitis viniferae planting percent | |
| CN102577698A (en) | Treatment method of white flower loropetalum chinense seeds | |
| CN103947434B (en) | A kind of method planting wild soybean on salt-soda soil | |
| CN102893847A (en) | Seedling raising substrate and cutting propagation method of bilberry plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Chen Dixin Inventor after: Deng Yuanping Inventor after: Liu Shengming Inventor after: Wu Zufeng Inventor after: Li Dongsheng Inventor after: Li Xiaojing Inventor before: Chen Dixin Inventor before: Deng Yuanping Inventor before: Li Xiaojing Inventor before: Wu Zufeng Inventor before: Li Dongsheng |
|
| CB03 | Change of inventor or designer information | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: CHEN DIXIN DENG YUANPING LI XIAOJING WU ZUFENG LI DONGSHENG TO: CHEN DIXIN DENG YUANPING LIU SHENGMING WU ZUFENG LI DONGSHENG LI XIAOJING |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150617 Termination date: 20160808 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |