CN107750511A - A kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition - Google Patents
A kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition Download PDFInfo
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- CN107750511A CN107750511A CN201710847021.0A CN201710847021A CN107750511A CN 107750511 A CN107750511 A CN 107750511A CN 201710847021 A CN201710847021 A CN 201710847021A CN 107750511 A CN107750511 A CN 107750511A
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- pear tree
- seed
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- lifting
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- 235000014443 Pyrus communis Nutrition 0.000 title claims abstract description 89
- 240000001987 Pyrus communis Species 0.000 title claims abstract description 86
- 238000000034 method Methods 0.000 title claims abstract description 40
- 239000011669 selenium Substances 0.000 title claims abstract description 36
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 30
- 230000007613 environmental effect Effects 0.000 title claims abstract description 19
- 238000002513 implantation Methods 0.000 title claims abstract description 19
- 238000012986 modification Methods 0.000 claims abstract description 12
- 230000004048 modification Effects 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 10
- 238000012545 processing Methods 0.000 claims description 54
- 238000011282 treatment Methods 0.000 claims description 51
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 35
- 238000007654 immersion Methods 0.000 claims description 21
- 230000008014 freezing Effects 0.000 claims description 17
- 238000007710 freezing Methods 0.000 claims description 17
- 238000001035 drying Methods 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 235000001014 amino acid Nutrition 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 11
- 238000002525 ultrasonication Methods 0.000 claims description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 239000003513 alkali Substances 0.000 claims description 10
- -1 compound amino acid Chemical class 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 5
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 5
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 5
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 5
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 5
- 239000004472 Lysine Substances 0.000 claims description 5
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 5
- 235000013922 glutamic acid Nutrition 0.000 claims description 5
- 239000004220 glutamic acid Substances 0.000 claims description 5
- 229920001661 Chitosan Polymers 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 4
- 229920001542 oligosaccharide Polymers 0.000 claims description 4
- 150000002482 oligosaccharides Chemical class 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 2
- VAJVDSVGBWFCLW-UHFFFAOYSA-N 3-Phenyl-1-propanol Chemical compound OCCCC1=CC=CC=C1 VAJVDSVGBWFCLW-UHFFFAOYSA-N 0.000 claims 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims 1
- DYUQAZSOFZSPHD-UHFFFAOYSA-N Phenylpropyl alcohol Natural products CCC(O)C1=CC=CC=C1 DYUQAZSOFZSPHD-UHFFFAOYSA-N 0.000 claims 1
- 229910021529 ammonia Inorganic materials 0.000 claims 1
- 229930182817 methionine Natural products 0.000 claims 1
- 235000013399 edible fruits Nutrition 0.000 abstract description 10
- 239000003337 fertilizer Substances 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 7
- 230000006872 improvement Effects 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 239000002689 soil Substances 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 16
- 230000000694 effects Effects 0.000 description 12
- 229910001385 heavy metal Inorganic materials 0.000 description 11
- 241000220324 Pyrus Species 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 235000021017 pears Nutrition 0.000 description 5
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 4
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002420 orchard Substances 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
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- 210000004185 liver Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
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- 238000003672 processing method Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010013789 Dry throat Diseases 0.000 description 1
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- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
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- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
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- 230000035784 germination Effects 0.000 description 1
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- 238000010438 heat treatment Methods 0.000 description 1
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- 231100000283 hepatitis Toxicity 0.000 description 1
- 238000002354 inductively-coupled plasma atomic emission spectroscopy Methods 0.000 description 1
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- 206010022437 insomnia Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 239000002362 mulch Substances 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition, special modification is carried out to pear tree seed specifically in planting process, is comprised the following steps：（1）Pretreatment,（2）Modification.The present invention has carried out special improvement to the implantation methods of pear tree and handled, influence of the unsuitable environmental condition for Growth of Pear Trees can be effectively reduced, and the Se content that absorbs characteristic, improve fruit in of the pear tree for selenium fertilizer can be lifted, there is good economic benefit and promotional value.
Description
Technical field
The invention belongs to pear tree planting technology field, and in particular to one kind lifting pear tree selenium-rich energy under unsuitable environmental condition
The implantation methods of power.
Background technology
Pears are known as the laudatory title of " ancestors of all kinds of fruits ".《Compendium of Materia Medica》Described in：Pears " the cool heart of moistening lung, anti-inflammatory fall fire, solution sore,
Wine poison ".Its medicinal can promote the production of body fluid cough-relieving, moistening dryness and resolving phlegm, relax bowel, to pyreticosis Tianjin wound, restlessness and thirst, dryness of the lung dry cough, dry throat tongue
The symptoms such as dry, choke,hiccup and regurgitation, dry and hard excrement have certain adjustment effect.In addition, it also has the effect of heat-clearing, calm, for height
Blood pressure, heart disease, have a dizzy spell, the patient such as insomnia and dreamful sleep has preferable booster action, it or hepatitis, the autumn of nephrotic
Health food, there are preferable liver protection, nourishing the liver and digestive effect.
Selenium, it is a kind of important raw material of industry, while is also the biogenic 14 kinds of trace elements of humans and animals
One of.In recent years, with the development of molecular biology, immunology and nutrition, the effect of selenium cause biology, medical science, environment,
The great attention of the every field scientist such as geochemistry, health care.Therefore Selenium In Foods content is improved to have great importance.
The growth of pear tree be unable to do without soil naturally, but along with the further development of economic globalization, mining and smelting
Refining, motor vehicle exhaust emission, municipal refuse are stacked, the discharge of Industrial " three Waste " and agricultural chemicals containing heavy metal, chemical fertilizer and mulch film
It is unreasonable the human factor such as to use to exacerbate heavy metal pollution of soil.Heavy metal pollution of soil have disguise, latency, for a long time
The characteristics of property and irreversibility.Heavy metal contaminants can not only destroy soil ecosystem, reduce crop yield, and can
In crop cylinder accumulation, to jeopardize the health of the mankind by food chain.The soil of heavy metals exceeding standard brings bad to pear tree
Growing environment.In order to lift the Se content in pear fruit, people are more to be added using selenium fertilizer, and then to carry by extra in plantation
Absorption enriching quantity of the high pear tree for selenium element, but because having complicated repercussion effect between each element, in heavy metals exceeding standard
Soil in addition apply selenium fertilizer often do not reach effectively lifting pear fruit in Se content purpose because heavy metal can reduce pears
Set and absorb effect for selenium fertilizer, so as to have impact on the benefit of plantation, also there are no the skill for effectively solving this problem at present
Art means.
The content of the invention
The purpose of the present invention is to be directed to the problem of existing, there is provided one kind lifting pear tree selenium-rich energy under unsuitable environmental condition
The implantation methods of power.
The present invention is achieved by the following technical solutions：
A kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition, to pear tree kind specifically in planting process
Son carries out special modification, comprises the following steps：
（1）Pretreatment：
Pear tree seed is first put into 5 ~ 7min of immersion treatment in acid solution, after the completion of take out place into immersion treatment 4 in alkali lye ~
6min, after the completion of take out place into 10 ~ 15min of immersion treatment in clear water, finally take out dry it is standby；
（2）Modification：
A. by step（1）Pear tree seed after processing is put into 40 ~ 50min of immersion treatment in treatment fluid A, after the completion of take out it is standby
With；Each composition and its corresponding parts by weight are in the treatment fluid A：5 ~ 7 parts of glucose, 1 ~ 3 part of compound amino acid, 2 ~ 4 parts of shell widows
Sugar, 100 ~ 120 parts of water；Glucose, compound amino acid, the chitosan oligosaccharide of addition not only increase containing for nutriment in pears seed
Amount, beneficial to follow-up sprouting, while it can also strengthen the protection for seed cell and active component, reduce follow-up microwave
Degree of injury；
B. the pear tree seed after a processing will be operated take out and be put into drying process in drying box, treat pear tree seed water content for 34 ~
Taken out after 38% standby；
C. the pear tree seed after b processing will be operated to be put into household freezer and carry out freezing processing, taken after 30 ~ 35min of freezing processing
Go out standby；
D. the pear tree seed after c is handled will be operated to be put into microwave treatment instrument, microwave treatment is carried out to seed, controls microwave
Power is 1.8 ~ 2.0kW, is taken out after 4.5 ~ 5.5s of microwave treatment standby；
E. to operation d processing after pear tree seed repeat once-through operation a, operation b, operation c, operation d processing after it is standby；
F. the pear tree seed after e is handled will be operated to be put into ultrasonication instrument, ultrasonication is carried out to seed, control is super
The frequency of sound wave is 1 ~ 1.1kHz, is taken out after 2 ~ 3min of supersound process standby；
G. the pear tree seed after f is handled will be operated and be put into progress vernalization processing in insulating box, treat that more than 85% seed splits
Vernalization is completed during mouth, and seed is taken out.
Further, step（1）Described in acid solution be mass fraction be 5 ~ 7% hydrochloric acid solution.
Further, step（1）Described in alkali lye be mass fraction be 6 ~ 8% sodium hydroxide solution.
Further, step（2）Operate in the compound amino acid described in a containing lysine, tryptophan, glutamic acid, egg
Propylhomoserin, phenylalanine.
Further, step（2）The temperature for operating the drying process described in b is 75 ~ 80 DEG C.
Further, step（2）The temperature for operating the freezing processing described in c is -18 ~ -22 DEG C.
Further, step（2）Vernalization described in operation g controls the temperature in insulating box when handling be 24 ~ 26 DEG C, phase
It is 70 ~ 75% to humid control.
Grown under the bad environmental conditions such as the higher soil of such as content of beary metal, the state of growing of pear tree can be by
To large effect, and the problem of be limited to technology and condition, the heavy metal element in soil can not be removed effectively in many cases,
The cultivation of pear tree is carried out in this soil can influence the benefit of plantation, while the selenium fertilizer applied can not also play due effect,
Special improvement is carried out to this present invention to the implantation methods of pear tree to handle, specifically the initial stage in plantation, to pear tree seed
Processing is modified, nursery is carried out with the seed after this processing, it is corresponding to have brought out the pear tree of high-quality, and then improve plantation
Benefit, wherein when being modified processing to seed, first carry out treatment fluid A immersion treatments to seed, it is effective in treatment fluid A
Composition is penetrated into pear tree seed, and its main function is the stability for lifting seed inner cell, enzyme etc., reduces follow-up high intensity
The infringement that microwave treatment is come to seed belt, then it will carry out freezing processing operation again after seed drying to certain water content, zero
Under low temperature environment by Interior Seed and the moisture freezing and crystallizing on top layer, improve to material stabilities such as seed inner cell, enzymes
Protection, reduce further the infringement that the processing of follow-up high-intensity microwave comes to seed belt, high intensity then carried out to seed
Microwave treatment, this microwave treatment can produce specific Mutagenesis effect to seed, reduce pear tree seed for heavy metal
The absorption characteristic of element, and the adverse-resistant characteristic of its plant is improved, significantly reduce containing high content heavy metal soil environment pair
In the side effect of Growth of Pear Trees, the application using effect of selenium fertilizer is enhanced, has then carried out ultrasonication operation again, effectively
The activity of a variety of enzymes in seed is improved, enhances the germination characteristic of seed, promotes the growth of follow-up plant.
The present invention has advantages below compared with prior art：
The present invention has carried out special improvement to the implantation methods of pear tree and handled, and can effectively reduce unsuitable environmental condition for pears
The influence of growth is set, and the Se content that absorbs characteristic, improve fruit in of the pear tree for selenium fertilizer can be lifted, is had fine
Economic benefit and promotional value.
Embodiment
Embodiment 1
A kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition, to pear tree kind specifically in planting process
Son carries out special modification, comprises the following steps：
（1）Pretreatment：
Pear tree seed is first put into immersion treatment 5min in acid solution, after the completion of take out place into immersion treatment 4min in alkali lye, it is complete
Immersion treatment 10min in clear water is placed into rear taking-up, finally taking-up is dried standby；
（2）Modification：
A. by step（1）Pear tree seed after processing is put into immersion treatment 40min in treatment fluid A, after the completion of take out it is standby；Institute
Stating each composition and its corresponding parts by weight in treatment fluid A is：5 parts of glucose, 1 part of compound amino acid, 2 parts of chitosan oligosaccharides, 100 parts of water；
B. the pear tree seed after a processing will be operated take out and be put into drying process in drying box, treat pear tree seed water content for 34 ~
Taken out after 35% standby；
C. the pear tree seed after b processing will be operated to be put into household freezer and carry out freezing processing, taken out after freezing processing 30min standby
With；
D. the pear tree seed after c is handled will be operated to be put into microwave treatment instrument, microwave treatment is carried out to seed, controls microwave
Power is 1.8kW, is taken out after microwave treatment 4.5s standby；
E. to operation d processing after pear tree seed repeat once-through operation a, operation b, operation c, operation d processing after it is standby；
F. the pear tree seed after e is handled will be operated to be put into ultrasonication instrument, ultrasonication is carried out to seed, control is super
The frequency of sound wave is 1kHz, is taken out after supersound process 2min standby；
G. the pear tree seed after f is handled will be operated and be put into progress vernalization processing in insulating box, treat that more than 85% seed splits
Vernalization is completed during mouth, and seed is taken out.
Further, step（1）Described in acid solution be mass fraction be 5% hydrochloric acid solution.
Further, step（1）Described in alkali lye be mass fraction be 6% sodium hydroxide solution.
Further, step（2）Operate in the compound amino acid described in a containing lysine, tryptophan, glutamic acid, egg
Propylhomoserin, phenylalanine.
Further, step（2）The temperature for operating the drying process described in b is 75 DEG C.
Further, step（2）The temperature for operating the freezing processing described in c is -18 DEG C.
Further, step（2）Vernalization described in operation g controls the temperature in insulating box when handling be 24 DEG C, relatively
Humid control is 70 ~ 72%.
Embodiment 2
A kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition, to pear tree kind specifically in planting process
Son carries out special modification, comprises the following steps：
（1）Pretreatment：
Pear tree seed is first put into immersion treatment 6min in acid solution, after the completion of take out place into immersion treatment 5min in alkali lye, it is complete
Immersion treatment 13min in clear water is placed into rear taking-up, finally taking-up is dried standby；
（2）Modification：
A. by step（1）Pear tree seed after processing is put into immersion treatment 42min in treatment fluid A, after the completion of take out it is standby；Institute
Stating each composition and its corresponding parts by weight in treatment fluid A is：6 parts of glucose, 2 parts of compound amino acids, 3 parts of chitosan oligosaccharides, 110 parts of water；
B. the pear tree seed after a processing will be operated take out and be put into drying process in drying box, treat pear tree seed water content for 36 ~
Taken out after 37% standby；
C. the pear tree seed after b processing will be operated to be put into household freezer and carry out freezing processing, taken out after freezing processing 33min standby
With；
D. the pear tree seed after c is handled will be operated to be put into microwave treatment instrument, microwave treatment is carried out to seed, controls microwave
Power is 1.9kW, is taken out after microwave treatment 5s standby；
E. to operation d processing after pear tree seed repeat once-through operation a, operation b, operation c, operation d processing after it is standby；
F. the pear tree seed after e is handled will be operated to be put into ultrasonication instrument, ultrasonication is carried out to seed, control is super
The frequency of sound wave is 1.05kHz, is taken out after supersound process 2.5min standby；
G. the pear tree seed after f is handled will be operated and be put into progress vernalization processing in insulating box, treat that more than 85% seed splits
Vernalization is completed during mouth, and seed is taken out.
Further, step（1）Described in acid solution be mass fraction be 6% hydrochloric acid solution.
Further, step（1）Described in alkali lye be mass fraction be 7% sodium hydroxide solution.
Further, step（2）Operate in the compound amino acid described in a containing lysine, tryptophan, glutamic acid, egg
Propylhomoserin, phenylalanine.
Further, step（2）The temperature for operating the drying process described in b is 78 DEG C.
Further, step（2）The temperature for operating the freezing processing described in c is -20 DEG C.
Further, step（2）Vernalization described in operation g controls the temperature in insulating box when handling be 25 DEG C, relatively
Humid control is 72 ~ 74%.
Embodiment 3
A kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition, to pear tree kind specifically in planting process
Son carries out special modification, comprises the following steps：
（1）Pretreatment：
Pear tree seed is first put into immersion treatment 7min in acid solution, after the completion of take out place into immersion treatment 6min in alkali lye, it is complete
Immersion treatment 15min in clear water is placed into rear taking-up, finally taking-up is dried standby；
（2）Modification：
A. by step（1）Pear tree seed after processing is put into immersion treatment 50min in treatment fluid A, after the completion of take out it is standby；Institute
Stating each composition and its corresponding parts by weight in treatment fluid A is：7 parts of glucose, 3 parts of compound amino acids, 4 parts of chitosan oligosaccharides, 120 parts of water；
B. the pear tree seed after a processing will be operated take out and be put into drying process in drying box, treat pear tree seed water content for 34 ~
Taken out after 38% standby；
C. the pear tree seed after b processing will be operated to be put into household freezer and carry out freezing processing, taken out after freezing processing 35min standby
With；
D. the pear tree seed after c is handled will be operated to be put into microwave treatment instrument, microwave treatment is carried out to seed, controls microwave
Power is 2.0kW, is taken out after microwave treatment 5.5s standby；
E. to operation d processing after pear tree seed repeat once-through operation a, operation b, operation c, operation d processing after it is standby；
F. the pear tree seed after e is handled will be operated to be put into ultrasonication instrument, ultrasonication is carried out to seed, control is super
The frequency of sound wave is 1.1kHz, is taken out after supersound process 3min standby；
G. the pear tree seed after f is handled will be operated and be put into progress vernalization processing in insulating box, treat that more than 85% seed splits
Vernalization is completed during mouth, and seed is taken out.
Further, step（1）Described in acid solution be mass fraction be 7% hydrochloric acid solution.
Further, step（1）Described in alkali lye be mass fraction be 8% sodium hydroxide solution.
Further, step（2）Operate in the compound amino acid described in a containing lysine, tryptophan, glutamic acid, egg
Propylhomoserin, phenylalanine.
Further, step（2）The temperature for operating the drying process described in b is 80 DEG C.
Further, step（2）The temperature for operating the freezing processing described in c is -22 DEG C.
Further, step（2）Vernalization described in operation g controls the temperature in insulating box when handling be 26 DEG C, relatively
Humid control is 74 ~ 75%.
Comparative example 1
This comparative example 1 compared with Example 2, saves step（2）In operation a processing, method and step in addition is homogeneous
Together.
Comparative example 2
This comparative example 2 compared with Example 2, saves step（2）In operation c processing, method and step in addition is homogeneous
Together.
Comparative example 3
This comparative example 3 compared with Example 2, saves step（2）In operation d processing, method and step in addition is homogeneous
Together.
Comparative example 4
This comparative example 4 compared with Example 2, saves step（2）In operation a and operation c processing, method in addition walk
Rapid all same.
Control group
Existing pear tree seed plants processing method.
In order to contrast effect of the present invention, from a collection of obtained pear tree seed as experimental subjects, respectively with above-mentioned reality
Apply example 2, comparative example 1, comparative example 2, comparative example 3, comparative example 4, at the method described in control group
Reason, the healthy and strong seedling being then bred as to each group carry out transplanting experiment, to the initial soil nutrient situation in plantation orchard before transplanting
It is measured, concrete operations are：First take soil sample from plantation orchard, sampling depth is 0 ~ 20cm, soil sample through removal plant residue,
Air-dry, after mixing, ground 1mm sieves are standby, then carry out physicochemical property test, and soil pH is measured with pHS-4C+ types Accurate pH
Fixed, the content of organic matter is determined with the Outside Heating Method of potassium bichromate titrimetric method one, and CEC is determined using ammonium acetate method, full nitrogen semimicro
Kelvin method is determined, and alkali-hydrolyzable nitrogen is determined with alkaline hydrolysis diffusion method, soil quick-effective phosphor 0.5molL-1 NaHCO3Method determines, effective potassium
Use 2molL-1HNO3Extract a flame spectrophotometric determination, full Cd HCl-HNO3- HF-HClO4Resolution, sampling Graphite Furnace Atomic Absorption
Spectrophotometer method（NovAA400- Jena, Germanies）Measure, full Pb ICP-AES（TJA, USA）Measure.Specific data such as following table
Shown in 1：
Table 1
| pH | CEC（cmol/ kg） | Organic matter OM （g/ kg） | Full nitrogen（g/ kg） | Alkali-hydrolyzable nitrogen（mg/ kg） | Available phosphorus（mg/ kg） | Effective potassium（mg/ kg） | Quan Ge（mg/ kg） | Full lead（mg/ kg） |
| 6.56 | 16.4 | 44.9 | 0.31 | 168.2 | 15.4 | 100.8 | 2.09 | 6.16 |
Cadmium, lead content are higher in the soil in plantation orchard it can be seen from upper table 1 selected by the present invention, belong to moderate dirt
Dye.
Then to the healthy and strong pear tree seedling after transplant planting（2 ages）Identical selenium-enriching cultivation pipe is carried out according to a conventional method
Reason, specifically add amino acid selenium foliar fertilizer in growth period（Fruit Institute, Chinese Academy of Agricultural Sciences develops, the prosperous Hai Sheng in Anqiu
Thing fertilizer Co., Ltd produces, stoste selenium concentration 3%）500 times of liquid, quality survey finally is carried out to the Pear Fruit of next year growth
Examination, and cultivation data are counted, specific correction data is as shown in table 2 below：
Table 2
| Se content in fruit（mg/kg） | Per mu yield（kg） | |
| Embodiment 2 | 0.210 | 5610 |
| Comparative example 1 | 0.092 | 4880 |
| Comparative example 2 | 0.115 | 5070 |
| Comparative example 3 | 0.025 | 4350 |
| Comparative example 4 | 0.021 | 3700 |
| Control group | 0.033 | 4020 |
It is rich for pear tree to effectively reduce heavy metals in soil for present invention plantation processing method it can be seen from upper table 1
The influence that selenium is cultivated, effectively improves the Se content in Pear Fruit, improves the edible quality and the market competitiveness of fruit, together
When effectively improve the yield of plantation again, there is good economic benefit and promotional value.
Claims (7)
1. a kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition, it is characterised in that be specifically to plant
During special modification is carried out to pear tree seed, comprise the following steps：
（1）Pretreatment：
Pear tree seed is first put into 5 ~ 7min of immersion treatment in acid solution, after the completion of take out place into immersion treatment 4 in alkali lye ~
6min, after the completion of take out place into 10 ~ 15min of immersion treatment in clear water, finally take out dry it is standby；
（2）Modification：A. by step（1）Pear tree seed after processing is put into 40 ~ 50min of immersion treatment in treatment fluid A,
After the completion of take out it is standby；Each composition and its corresponding parts by weight are in the treatment fluid A：5 ~ 7 parts of glucose, 1 ~ 3 part of compounded amino
Acid, 2 ~ 4 parts of chitosan oligosaccharides, 100 ~ 120 parts of water；
B. the pear tree seed after a processing will be operated take out and be put into drying process in drying box, treat pear tree seed water content for 34 ~
Taken out after 38% standby；
C. the pear tree seed after b processing will be operated to be put into household freezer and carry out freezing processing, taken after 30 ~ 35min of freezing processing
Go out standby；
D. the pear tree seed after c is handled will be operated to be put into microwave treatment instrument, microwave treatment is carried out to seed, controls microwave
Power is 1.8 ~ 2.0kW, is taken out after 4.5 ~ 5.5s of microwave treatment standby；
E. to operation d processing after pear tree seed repeat once-through operation a, operation b, operation c, operation d processing after it is standby；
F. the pear tree seed after e is handled will be operated to be put into ultrasonication instrument, ultrasonication is carried out to seed, control is super
The frequency of sound wave is 1 ~ 1.1kHz, is taken out after 2 ~ 3min of supersound process standby；
G. the pear tree seed after f is handled will be operated and be put into progress vernalization processing in insulating box, treat that more than 85% seed splits
Vernalization is completed during mouth, and seed is taken out.
2. a kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition according to claim 1, it is special
Sign is, step（1）Described in acid solution be mass fraction be 5 ~ 7% hydrochloric acid solution.
3. a kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition according to claim 1, it is special
Sign is, step（1）Described in alkali lye be mass fraction be 6 ~ 8% sodium hydroxide solution.
4. a kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition according to claim 1, it is special
Sign is, step（2）Operate in the compound amino acid described in a containing lysine, tryptophan, glutamic acid, methionine, phenylpropyl alcohol ammonia
Acid.
5. a kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition according to claim 1, it is special
Sign is, step（2）The temperature for operating the drying process described in b is 75 ~ 80 DEG C.
6. a kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition according to claim 1, it is special
Sign is, step（2）The temperature for operating the freezing processing described in c is -18 ~ -22 DEG C.
7. a kind of implantation methods for lifting pear tree selenium rich ability under unsuitable environmental condition according to claim 1, it is special
Sign is, step（2）Vernalization described in operation g controls the temperature in insulating box when handling be 24 ~ 26 DEG C, relative humidity control
For 70 ~ 75%.
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| CN103430656A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Seedling culture method capable of relieving pear seeds from dormancy |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430656A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Seedling culture method capable of relieving pear seeds from dormancy |
Non-Patent Citations (4)
| Title |
|---|
| 农业部农民科技教育培训中心: "《现代蔬菜育苗技术》", 30 November 2007, 中国农业科学技术出版社 * |
| 梁建萍: "《植物学》", 28 February 2014, 中国农业出版社 * |
| 王建林: "《高级作物生理学》", 31 March 2013, 中国农业大学出版社 * |
| 邵燮麟: "《超声》", 28 February 1962, 中华人民共和国科学技术协会全国委员会 * |
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