CN102898225B - Composite manure water preparation and application thereof - Google Patents
Composite manure water preparation and application thereof Download PDFInfo
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- CN102898225B CN102898225B CN201210342073.XA CN201210342073A CN102898225B CN 102898225 B CN102898225 B CN 102898225B CN 201210342073 A CN201210342073 A CN 201210342073A CN 102898225 B CN102898225 B CN 102898225B
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- rich water
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Abstract
The invention relates to a composite manure water preparation which comprises the following substances by weight percent: 80 to 92 % of decomposed chicken manure, 3 to 10 % of calcium superphosphate, 1 to 5 % of potassium dihydrogen phosphate, 0.5 to 5 % of calcium carbonate, 0.02 to 0.35 % of zinc sulfate, 0.04 to 0.70 % of manganese sulphate, 0.02 to 0.2 % of manganese chloride, 0.1 to 0.6 % of copper sulfate, 0.1 to 0.7 % of ferrous sulfate heptahydrate, 0.02 to 0.2 % ammonium molybdate, 0.02 to 0.35% of the boron ions and 0.5 to 1 % of cyclotella. In addition, the composite manure water preparation provided by the invention is added with bacterial liquid of Bacillus subtilis, lactic acid bacteria and bifidobacteria. According to the invention, by using the fermented duck manure as main raw materials, together with N, P, K, trace elements, etc. necessary for phytoplankton and zooplankton, the prepared organic manure water preparation is sufficient in organic nutrients and trace element nutrients, and reasonable in compatibility, and can quickly supplement small molecular organic debris needed by pearl mussel, and N, P, K and a variety of trace elements needed by the phytoplankton and the zooplankton.
Description
Technical field
The invention belongs to agriculture production product technique field, be specifically related to a kind of rich water compound formulation and application thereof, the rich water compound formulation that a kind of applicable pearl culturing clam cultivation waters is used.
Background technology
At present, the rich water product of culture fishery is a lot, but mostly configures for fishery products such as fishes and shrimps.And the cultivation of pearl freshwater mussel requires with general breed variety difference, current rich water product can not meet the demand of pearl freshwater mussel to basic nutritive elements such as nitrogen, phosphorus, potassium, calcium.And, also have a lot of mineral elements and trace element also very important at the foster freshwater mussel fishing waters of growing cultured pearls, being formed with directly and indirectly of the growth on pearl culturing clam and pearl affects.For example, nitrogen, phosphorus, potassium are the necessary elements of phytoplankton growth and breeding, and plant plankton is the primary productivity of water body, and plankton and organic debris are the basic substances that pearl culturing clam is depended on for existence.When food organism in water are enriched, the nutritional sufficiency of pearl culturing clam, pearl also just looks soon, and quality is high.It is different that this and general aquaculture need to be thrown in bait.Consider that calcium (existing with calcium carbonate form) is the major ingredient of pearl culturing clam shell and pearl, therefore, the calcium contents that the aquaculture water of pearl culturing clam requires is higher than ortho-water product demand.Meanwhile, all require certain content for copper, zinc, manganese, iron etc.Therefore develop for the special rich water preparation of pearl culturing clam aquaculture water and there is very great meaning.
Summary of the invention
The object of this invention is to provide the rich water compound formulation that a kind of applicable pearl culturing clam cultivation waters is used, have that calcic is high, nutritious, fertilizer efficiency is both lasting rapidly, can go out plant plankton and zooplankton by high effect culture.
Rich water compound formulation of the present invention, includes the material of following weight percent: the duck excrement 80~92% that becomes thoroughly decomposed, calcium superphosphate 3~10%, potassium primary phosphate 1~5%, calcium carbonate 0.5~5%, zinc sulfate 0.02~0.35%, manganous sulfate 0.04~0.70%, Manganous chloride tetrahydrate 0.02~0.2%, copper sulfate 0.1~0.6%, iron vitriol 0.1~0.7%, ammonium molybdate 0.02~0.2%, boron ion 0.02~0.35%, little ring algae 0.5~1%.
Rich water compound formulation of the present invention, is also added with the bacterium liquid of genus bacillus, milk-acid bacteria and bifidus bacillus, makes genus bacillus, milk-acid bacteria and the bifidus bacillus quantity in compound formulation be respectively 10
6~10
8cFU/kg.
The present invention utilizes duck excrement after fermentation as main raw material, coordinates plant plankton and the necessary N of zooplankton, P, K and trace element etc., the fertilizer water preparation of making, and organic nutrient and micronutrient abundance, coordinate rationally.Use the advantage of rich water compound formulation of the present invention as follows:
(1) the present invention can supplement the required small molecules organic debris of pearl culturing clam and plankton needs N, P, K and various trace elements fast.
(2) part material of the present invention is taken from and is supported the waste material that duck industry produces, and turns waste into wealth, and avoided a large amount of wastings of resources, and preparation is simple and easy to use.
(3) the present invention can be used for green, organic foodstuff production.
Embodiment
The preparation method of duck excrement of becoming thoroughly decomposed adopts traditional method of naturally becoming thoroughly decomposed, and after duck excrement is piled up, becomes thoroughly decomposed, and stirs therebetween, and to duck excrement odorless, the brown that darkens to black has just completed the preparation of the duck excrement that becomes thoroughly decomposed.
For the specific content that increases little ring algae, in rich water agent of the present invention, be added with the live body of a small amount of little ring algae as algae kind, in the time using, can effectively improve like this amount of little ring algae in aquaculture water, for the growth of pearl culturing clam provides sufficient bait.
In order to strengthen the effect of rich water preparation of the present invention, in the rich water preparation of preparation, be also optionally added into the viable bacteria of genus bacillus, milk-acid bacteria and bifidus bacillus.Wherein the substratum of genus bacillus is composed as follows: 1L distilled water+20g glucose+15g peptone+5g sodium-chlor+0.5g extractum carnis+20g agar;
Milk-acid bacteria substratum (MRS solid medium) is as follows: peptone 10g, extractum carnis 10g, yeast extract paste 5g, K
2hPO
43H
2o 2g, sodium acetate 5g, glucose 20g, tween 80 1g, dibasic ammonium citrate 2gMgSO
47H
2o 0.58g, MnSO
44H
2o 0.25g, adding distil water 1000mL, adjusts PH6.2-6.4 at 121 DEG C, and 15min sterilizing is for subsequent use;
Medium of Bifidobacterium is selected BS substratum, can buy from Qingdao Hai Bo Bioisystech Co., Ltd.
After the substratum of genus bacillus, milk-acid bacteria, bifidus bacillus is prepared, respectively bacterial classification is inoculated in substratum according to 2% inoculum size, after access, after 35 DEG C of incubated overnight 24~48h, or measuring OD600 value is within 0.5 o'clock, to stop cultivating.
Below in conjunction with embodiment, the present invention is described further and verification experimental verification.
Embodiment 1
The rich water preparation of the present embodiment, raw material by following weight percent is made: fermentation duck excrement 91%, calcium superphosphate 3%, potassium primary phosphate 2.3%, calcium carbonate 0.5%, zinc sulfate 0.2%, manganous sulfate 0.3%, Manganous chloride tetrahydrate 0.2%, copper sulfate 0.5%, iron vitriol 0.5%, ammonium molybdate 0.2%, boric acid 0.3%.The said products is added to stirrer stirring and evenly mixing.Then add the algae liquid of little ring algae, air-dry, making the weight percent of little ring algae in rich water agent is 1%.
Little ring algae is bought from hydrobiont institute of Wuhan Chinese Academy of Sciences algae kind storehouse, is numbered FACHB-1030, and substratum is selected D1 substratum.
Embodiment 2
The present embodiment and embodiment 1 difference are that the mass percent of raw material is different, and its preparation method is identical with embodiment 1.
Fermentation duck excrement 87.5%, calcium superphosphate 5%, potassium primary phosphate 3.8%, calcium carbonate 0.5%, zinc sulfate 0.3%, manganous sulfate 0.6%, Manganous chloride tetrahydrate 0.1%, copper sulfate 0.6%, iron vitriol 0.7%, ammonium molybdate 0.2%, boric acid 0.2%, little ring algae 0.5%.
Embodiment 3
The present embodiment and embodiment 1 difference are that the mass percent of raw material is different, and its preparation method is identical with embodiment 1.
Fermentation duck excrement 87.5%, calcium superphosphate 5%, potassium primary phosphate 3.8%, calcium carbonate 0.5%, zinc sulfate 0.3%, manganous sulfate 0.6%, Manganous chloride tetrahydrate 0.1%, copper sulfate 0.6%, iron vitriol 0.7%, ammonium molybdate 0.2%, boric acid 0.2%, little ring algae 0.5%.
Preparing after above-mentioned rich water preparation, the cultivation bacterium liquid of genus bacillus, milk-acid bacteria, bifidus bacillus is being added to stirring, making the quantity of three kinds of bacterium in compound formulation be respectively 10
6~10
8cFU/kg, makes the rich water agent of the present embodiment after being then dried.Wherein genus bacillus can be selected purchased from Chinese agriculture microbial strains preservation center, is numbered the bacterial strain of 1511C0001ACCC02728; Bifidus bacillus, purchased from Chinese agriculture microbial strains preservation center, is numbered 1511C0001ACCC11054; Milk-acid bacteria also, purchased from Chinese agriculture microbial strains preservation center, is numbered 1511C0001ACCC10637.
Owing to having added the viable bacteria of probiotics in rich water agent, before rich water agent is used, bacterium also can carry out biological degradation processing again to fermentation duck excrement; Therefore, compare than the rich water agent of not adding bacterium, when use, have extra beneficial effect.
The using method of rich water compound formulation of the present invention:
By preparation water 1:10 dilution, the culture pond of splashing after being uniformly mixed, 1 meter of pond usage quantity of every mu of depth of water is 1-3Kg.Concrete usage quantity is depending on water colour, and the season of growth of the 5-10 month, every two weeks once.
The experimental study of rich water compound formulation:
Water test is trained in pearl freshwater mussel cultivation waters in the world, Shaoxing, Zhejiang profit company, 22 DEG C of water temperatures, 1 meter of the depth of water, the water surface is 5 mu, uses the rich water compound formulation 10Kg of embodiment 1, test is fetched water after the same day and 10 days 1 part of each pond surrounding and central each water sampling, fully mix, get 1 L mixed sample and pour vial into, add 5% formaldehyde solution to fix, leave standstill 24-48h, concentrated after at plant plankton counting frame microscopy, counting.Result shows, the planktonic microalgae cell quantity in water body is 4 times before using, and little ring algae number preponderates, and rolled up the bait of pearl culturing clam, lays a solid foundation for bringing out high quality pearl.
Adopt the rich water agent of embodiment 2,3 to carry out identical test, also can reach rich water effect of the present invention, the object that provides pearl culturing clam to grow required plant plankton and zooplankton is provided.And owing to having added probiotics in embodiment 3, in cultivation, nacreous sickness rate is all lower than the preparation of embodiment 1 and 2.
Claims (3)
1. the rich water compound formulation that applicable pearl culturing clam cultivation waters is used, is characterized in that, includes the material of following weight percent: duck excrement 80~92% becomes thoroughly decomposed, calcium superphosphate 3~10%, potassium primary phosphate 1~5%, calcium carbonate 0.5~5%, zinc sulfate 0.02~0.35%, manganous sulfate 0.04~0.70%, Manganous chloride tetrahydrate 0.02~0.2%, copper sulfate 0.1~0.6%, iron vitriol 0.1~0.7%, ammonium molybdate 0.02~0.2%, boron ion 0.02~0.35%, little ring algae 0.5~1%.
2. rich water compound formulation as claimed in claim 1, is characterized in that described rich water compound formulation is also added with genus bacillus, milk-acid bacteria and bifidus bacillus.
3. rich water compound formulation as claimed in claim 2, is characterized in that described genus bacillus, milk-acid bacteria and the bifidus bacillus quantity in compound formulation is respectively 10
6~10
8cFU/kg.
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Citations (2)
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---|---|---|---|---|
CN1410397A (en) * | 2002-12-06 | 2003-04-16 | 赵鹏 | Biological organic and inorganic composite fertilizer and its production method |
CN101225007A (en) * | 2008-02-03 | 2008-07-23 | 武汉市科洋生物工程有限公司 | Special biological fish-fertilizer for aquaculture and preparation method thereof |
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2012
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Publication number | Priority date | Publication date | Assignee | Title |
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CN1410397A (en) * | 2002-12-06 | 2003-04-16 | 赵鹏 | Biological organic and inorganic composite fertilizer and its production method |
CN101225007A (en) * | 2008-02-03 | 2008-07-23 | 武汉市科洋生物工程有限公司 | Special biological fish-fertilizer for aquaculture and preparation method thereof |
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