CN102628874B - Full-automatic colloidal gold detection method - Google Patents
Full-automatic colloidal gold detection method Download PDFInfo
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- CN102628874B CN102628874B CN 201210132692 CN201210132692A CN102628874B CN 102628874 B CN102628874 B CN 102628874B CN 201210132692 CN201210132692 CN 201210132692 CN 201210132692 A CN201210132692 A CN 201210132692A CN 102628874 B CN102628874 B CN 102628874B
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Abstract
The invention provides a full-automatic colloidal gold detection instrument which comprises a controller, a detection card conveying platform, a constant-temperature incubator, a cassette, a CCD (Charge Coupled Device) image collector, an image collector positioning guide rail and a waste card collection box, wherein the detection card conveying platform adopts a conveying belt or a conveying disc, the conveying belt or the conveying disc is driven by a servo motor or a stepping motor; detection cards are sequentially placed at conveying positions of the conveying belt or the conveying disc; an image collector is arranged above one region of the conveying belt or the conveying disc and is positioned on the guide rail; a detection card accommodating region is arranged at the input part of the conveying belt or the conveying disc, and the constant-temperature incubator is behind the detection card accommodating region. According to the full-automatic colloidal gold detection instrument, a continuous sampling device is additionally arranged, so that the detection speed is greatly improved; and a positioning track system of the image collector is additionally arranged, so that the collection position of the image collector is optimized, the reaction speed is quickened, the detection process is reasonable and reliable, the test result is enabled to be accurate.
Description
One, technical field
The present invention relates to automatic testing method and the device of Biosample, especially collaurum detection method and test card arrangement for reading.
Two, background technology
The collaurum test card is that specific antigen or antibody (detect and use reagent) are fixed on the film with ribbon (coating), colloid gold label reagent (antibody or monoclonal antibody) is adsorbed on the pad, after sample to be checked is added on the sample pad of test strips one end in the test card, move forward by capillary action, react to each other behind the colloid gold label reagent on the dissolving pad, when moving to fixing antigen or antibody regional again, specificity takes place with it again for thing to be checked and the bond of gold marked reagent is combined and is trapped, accumulate in to detect and be with, whether can develop the color by being observed visually (perhaps according to colour developing degree and colour atla contrast) judges testing result.
Existing collaurum test card reader, existing reader mainly is to be provided with a CCD(or CMOS a magazine inside) image acquisition device carries out image acquisition to the color development area of rapid detection card, by software to the graphical analysis of gathering with the decision analysis result, it is formed structure and generally is provided with a plug-in card hole (or a plug-type cartridge), the test card that will add sample during use is put into the plug-in card hole, and question response finishes the back card reader and gathers image automatically and calculate test result.
Though 1, existing card reader has solved and had judged the parallax error that produces by naked eyes in the past, but because it once can only place a slice test card, (be example with the clenobuterol hydrochloride and testing sample needs the certain reaction time after joining test card, need reaction three minutes), cause detection speed lower, can not satisfy batch samples the detection demand (at present the slaughterhouse required to each slaughter object all take a sample detect).
2, in addition because the reaction tag line of test card is generally all thinner, the markings position of each batch has differences between batches in process of production, and the CCD of card reader generally fixes, and the site error of tag line will produce certain influence to test result.
Three, summary of the invention
The present invention seeks to, propose a kind of continuous automatic inspection method and apparatus of Biosample, especially collaurum detection method and test card arrangement for reading.And improve the accuracy that collaurum detects.
Technical scheme of the present invention is: the collaurum detection method, the colloidal gold test card bar code is read, and image is gathered in advance, the markings location, and the fastening position moves to the optimum position, the precise image collection, image storage and analyzing and processing also go out result's report.
In specific words: the automatic transmission platform that is provided with the collaurum test card, the test card that will add reagent is placed on the automatic transmission platform, the mobile position of the every interval of transmission platform certain hour (time can preestablish), test card is hatched the district by constant temperature earlier, carries out image acquisition again; Image acquisition device is adjusted to best image capture position to image acquisition by the displacement regulating system.
Elder generation is through pre-acquisition zone before the image acquisition, pre-acquisition zone is determined the chromogenic line exact position of test card, subsequent picture collector C CD(or CMOS) displacement system is automatically with image acquisition device CCD(or CMOS) adjust to best image capture position, carry out the analysis image collection, and by software image analysis is judged testing result.
Ccd image collector displacement system is adjusted to the ccd image collector best image capture position automatically, carry out the analysis image collection, and by software image analysis is judged testing result, the test card of gathering image is the most at last delivered to useless card and is collected in the box.
Image acquisition device by the method for displacement regulating system is: image acquisition device is regulated by a location guide rail: hang and be displaced in the cross recess a certain position and make image acquisition device obtain chromogenic line exact position.
Transmission platform continued to move after detection was finished, and gathered the test card of image the most at last and delivered in the useless card collection box.Instrument can be set different parameters according to the kind of test card, as qualitative or quantitative test parameter of translational speed, image acquisition number of times and the chromogenic reaction of reaction time, transmission platform etc.
Automatically transmission platform is travelling belt or transmission dish.Device of the present invention is that full-automatic collaurum detector comprises: controller, test card transmission platform, and the constant temperature incubator, magazine, the ccd image collector, the image acquisition device positioning guide rail can also be provided with useless card in addition and collect box; The test card transmission platform adopts travelling belt or transmits disk, and travelling belt or transmission disk increase employing servomotor or stepper motor driving.Test card places travelling belt successively or transmits the delivering position of disk; Travelling belt or transmit disk a zone above be provided with image acquisition device, image acquisition device is positioned on the location guide rail; The input of travelling belt or transmission disk is provided with the test card rest area, is the constant temperature incubator after the test card rest area, is provided with useless card collection box at travelling belt or after transmitting disk image acquisition device zone.Positioning guide rail is provided with cross recess as the guide rail regulating device: hang and be displaced into that a certain position makes image acquisition device obtain chromogenic line exact position in the cross recess.Transmission platform continued to move after detection was finished, and gathered the test card of image the most at last and delivered in the useless card collection box.
Apparatus of the present invention can be set different parameters according to the kind of test card, as qualitative or quantitative test parameter of translational speed, image acquisition number of times and the chromogenic reaction of reaction time, transmission platform etc.
Beneficial effect of the present invention is: the inventive method and detector increase the continuous sample introduction device on existing conventional equipment basis, significantly improved detection speed, are user-friendly to.Increase the positioning track system of image acquisition device, determined the chromogenic line position by locating pre-collection, optimized the collection position of image acquisition device, eliminated the influence of test card chromogenic line error to testing result.Be provided with constant temperature at transporting rail and hatch device, fast reaction speed, testing process rationally reach efficient, guarantee the test result accuracy.
Four, description of drawings
Fig. 1 is pick-up unit synoptic diagram of the present invention;
Fig. 2 is the partial enlarged drawing of Fig. 1;
Fig. 3 is system architecture of the present invention;
Fig. 4 is process flow diagram of the present invention.
Five, embodiment
The collaurum detection method, be provided with the automatic transmission platform of collaurum test sample, the test card that will add reagent is placed on the automatic transmission platform, the mobile position of the every interval of transmission platform certain hour (time can preestablish), test card is hatched the district by constant temperature earlier, carries out image acquisition again; Image acquisition device is adjusted to best image capture position to image acquisition by the displacement regulating system.Pre-acquisition zone is determined the chromogenic line exact position of test card, subsequent picture collector C CD(or CMOS) displacement system is automatically with image acquisition device CCD(or CMOS) adjust to best image capture position, carry out the analysis image collection, and by software image analysis is judged testing result.
Shown in Figure 3, adopting the embedded computer control system is CPU, directly controls power of motor driving and sensor groups test, card mechanical transmission mechanism, temperature control module electric heating device.
Embedded computer is provided with human-computer interaction device: input (button, touch-screen, bar code scan) man-machine interaction output (liquid crystal display, built-in printer, hummer, prompting LED), external interface: RS-232, USB, network interface.
Flow process of the present invention is as follows: the test card bar code reads, and image is gathered in advance, the markings location, and the fastening position moves to the optimum position, precise image collection, image storage and analyzing and processing and result's report.
When the test card bar code reads by being provided with the automatic transmission platform of collaurum test sample, the test card that will add reagent is placed on the automatic transmission platform, the mobile position of the every interval of transmission platform certain hour (time can preestablish), test card can be hatched the district by constant temperature earlier, carries out image acquisition again; During image acquisition through image gather in advance, markings location, the fastening position moves to the optimum position and carries out the precise image collection again.Image acquisition device is adjusted to best image capture position to the meticulous collection of image by the displacement regulating system.
Elder generation is through pre-acquisition zone before the image acquisition, pre-acquisition zone is determined chromogenic line (markings) exact position of test card, subsequent picture collector C CD(or CMOS) displacement system is automatically with image acquisition device CCD(or CMOS) adjust to best image capture position, carry out the analysis image collection, and by software image analysis is judged testing result.
Ccd image collector displacement system is adjusted to the ccd image collector best image capture position automatically, carry out the analysis image collection, and by software image analysis is judged testing result, the test card of gathering image is the most at last delivered to useless card and is collected in the box.
During analyser work, the test card that only needs to have added sample is placed on the transmission platform, the mobile position of the every interval of transmission platform certain hour (time can preestablish), test card is hatched device by constant temperature earlier, enter magazine again, the ccd image collector carried out first time image earlier and gathered in advance this moment, pass through software analysis, determine the chromogenic line exact position of test card, ccd image collector displacement system is adjusted to the ccd image collector best image capture position automatically subsequently, carry out the analysis image collection, and by software image analysis is judged testing result, transmission platform continued to move after detection was finished, and gathered the test card of image the most at last and delivered in the useless card collection box.
The formation of apparatus of the present invention is: pilot lamp 1, guide rail 2, controller adopts little process chip (typical chip), integrated or establish storer in addition, its output is by controller (the typical chip model of control chip control step or driven by servomotor, being listed as under Xiao slightly and getting final product) constant temperature hatches the temperature controller of device, with by stepping or driven by servomotor track drive shaft 3, reference column 4, constant temperature oven 5, ccd image collector 6 adopts existing equipment, test card 7, useless card gatherer 9, the ccd image collector comprises analysis image acquisition system software (available present technology), and by software image analysis is judged testing result.The analysis image acquisition system can with controller integral of the present invention, the speed that controller control step or driven by servomotor guide rail drive is finished the qualitative or quantitative test parameter etc. of hatching translational speed, image acquisition number of times and the chromogenic reaction of humidity control, reaction time, transmission platform as constant temperature.
Claims (2)
1. collaurum detection method, it is characterized in that being provided with the automatic transmission platform of collaurum test sample, the test card that will add reagent is placed on the automatic transmission platform, and the every interval of transmission platform certain hour moves a position, test card is hatched the district by constant temperature earlier, carries out image acquisition again; Image acquisition device is adjusted to best image capture position to image acquisition by the displacement regulating system; Elder generation is through pre-acquisition zone before the image acquisition, pre-acquisition zone is determined the chromogenic line exact position of test card, subsequent picture collector C CD or CMOS displacement system are adjusted to best image capture position with image acquisition device CCD or CMOS automatically, carry out the analysis image collection; Image acquisition device by the method for displacement regulating system is: image acquisition device is regulated by a location guide rail, hangs and is displaced in the cross recess a certain position and make image acquisition device obtain chromogenic line exact position.
2. collaurum detection method according to claim 1 is characterized in that detecting and finishes the back transmission platform and continue mobilely, gathers the test card of image the most at last and delivers in the useless card collection box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210132692 CN102628874B (en) | 2012-04-28 | 2012-04-28 | Full-automatic colloidal gold detection method |
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| CN 201210132692 CN102628874B (en) | 2012-04-28 | 2012-04-28 | Full-automatic colloidal gold detection method |
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| CN102628874A CN102628874A (en) | 2012-08-08 |
| CN102628874B true CN102628874B (en) | 2013-07-03 |
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| CN 201210132692 Expired - Fee Related CN102628874B (en) | 2012-04-28 | 2012-04-28 | Full-automatic colloidal gold detection method |
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Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105759024B (en) * | 2013-07-15 | 2018-06-26 | 艾博生物医药(杭州)有限公司 | A kind of calibration method for the reading equipment for reading immunoassay device |
| CN103760338B (en) * | 2014-01-26 | 2016-02-03 | 杭州海士利生物科技有限公司 | Quantitative detection method using reverse immunochromatography reaction, kit and external equipment |
| CN104836942B (en) * | 2014-11-03 | 2017-12-19 | 中国计量学院 | The portable CCD readout instruments of collaurum |
| CN104714004B (en) * | 2015-03-11 | 2016-07-06 | 厦门为正生物科技股份有限公司 | Biological detection card integrated information acquisition method |
| CN105203723A (en) * | 2015-07-15 | 2015-12-30 | 宁波高新区绿邦科技发展有限公司 | Device and method for quickly and quantitatively detecting clenbuterol hydrochloride |
| CN105044336A (en) * | 2015-07-25 | 2015-11-11 | 中山瑞福医疗器械科技有限公司 | Multi-channel detecting instrument |
| CN108132346A (en) * | 2017-12-30 | 2018-06-08 | 天津博硕东创科技发展有限公司 | A kind of Portable fluorescence immunity analysis instrument |
| CN109490299A (en) * | 2018-09-04 | 2019-03-19 | 广东朗源生物科技有限公司 | A kind of portable mycotoxin colloidal gold detector and application method |
| CN109613286A (en) * | 2018-12-27 | 2019-04-12 | 宁波大学 | A kind of qualitative/quantitative bio-chip test device |
| CN109696439A (en) * | 2019-02-26 | 2019-04-30 | 长沙协大生物科技有限公司 | A kind of urine dry chemical scrip image collecting device |
| CN110220895A (en) * | 2019-06-28 | 2019-09-10 | 中国计量大学 | A kind of image collecting device of high throughput portable colloidal gold CCD readout instrument |
| CN110794151A (en) * | 2019-10-30 | 2020-02-14 | 中山生物工程有限公司 | Method, device, terminal, medium and equipment for detecting quality of blood type card |
| CN112485182A (en) * | 2020-11-16 | 2021-03-12 | 安徽省富鑫雅光电科技有限公司 | LED lamp panel detection device |
| CN115436650A (en) * | 2022-11-08 | 2022-12-06 | 山东康华生物医疗科技股份有限公司 | Full-automatic colloidal gold analyzer |
| CN117420292A (en) * | 2023-11-07 | 2024-01-19 | 青岛华晶生物技术有限公司 | Incubator |
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| US20050168747A1 (en) * | 2004-01-29 | 2005-08-04 | Fox Shaw G. | Information-encoded tests and method |
| US20070134812A1 (en) * | 2003-11-07 | 2007-06-14 | Guzman Norberto A | Controlled electrophoresis method |
| CN201051102Y (en) * | 2007-05-29 | 2008-04-23 | 陈晓汀 | Quick tester for gold colloid |
| CN102253195A (en) * | 2011-06-28 | 2011-11-23 | 上海贝西生物科技有限公司 | Full-automatic immunochromatographic analyzer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101551340B (en) * | 2009-05-07 | 2011-03-23 | 上海交通大学 | Gold spot quantitative determination optical device |
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|---|---|---|---|---|
| US20070134812A1 (en) * | 2003-11-07 | 2007-06-14 | Guzman Norberto A | Controlled electrophoresis method |
| US20050168747A1 (en) * | 2004-01-29 | 2005-08-04 | Fox Shaw G. | Information-encoded tests and method |
| CN201051102Y (en) * | 2007-05-29 | 2008-04-23 | 陈晓汀 | Quick tester for gold colloid |
| CN102253195A (en) * | 2011-06-28 | 2011-11-23 | 上海贝西生物科技有限公司 | Full-automatic immunochromatographic analyzer |
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