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CN106706937B - A kind of quasi- automated immunoassay analyzer and detection method - Google Patents

A kind of quasi- automated immunoassay analyzer and detection method Download PDF

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Publication number
CN106706937B
CN106706937B CN201710064718.0A CN201710064718A CN106706937B CN 106706937 B CN106706937 B CN 106706937B CN 201710064718 A CN201710064718 A CN 201710064718A CN 106706937 B CN106706937 B CN 106706937B
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reagent card
detection
quasi
sliding block
discharging
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CN106706937A (en
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王东元
武通园
汪德禄
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Shenzhen City Live Near Diagnosis Instrument Co Ltd Waterbed
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Shenzhen City Live Near Diagnosis Instrument Co Ltd Waterbed
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    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
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  • General Physics & Mathematics (AREA)
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Abstract

This application involves a kind of quasi- automated immunoassay analyzer and detection methods, including reagent card conveying device (1), incubating device (3), detection device (4), the detection device (4) is in plane A, the incubating device (3) and reagent card conveying device (1) are in same plane B, the reagent card conveying device (1) is incubated for for reagent card (2) to be successively transmitted to the incubating device (3), the detection device (4) is detected to the reagent card (2) completed is incubated for, at least two parallel card slots are provided on the incubating device (3), the direction of reagent card conveying device (1) the delivery of therapeutic agents card (2) and the motion profile of incubation detection platform (308) of the incubating device (3) are vertical.The structure of the application is simple, compact, occupies little space, and greatly reduces cost;Each component mutual cooperation, co-ordination under control system control, realizes instrument intelligent prompt and false alarm.

Description

A kind of quasi- automated immunoassay analyzer and detection method
Technical field
The present invention relates to a kind of quasi- automated immunoassay analyzer and detection methods, belong to the inspection of the immunoassays such as fluorescence and colloidal gold Survey head of a bed diagnostic field.
Background technique
To existing quasi- automated immunoassay analyzer, the automatic fluorescence immunity analyzer of standard of rotating disk mechanism is especially mostly used, There is following deficiency:
1 sample different in size for incubation time, analyzer can only uniformly be analyzed by same incubation time, be drawn Play inconvenient for operation and inefficiency;
The reagent that 2 rotating disk mechanisms are placed, interruption is transported to incubation mechanism, waiting for a long time inefficient, in the prior art Structure is incubated for by the way of turntable, is arranged radially between reagent card slot, when this kind of mode causes W-response to wait Between it is long, occupy volume it is larger.
3 in the prior art the general usual volume of immune quantitative detector it is larger, it is inconvenient to carry, and is not easy to examine immediately It tests, limits it and having inconvenient traffic, the development of under-developed area.
4 in the prior art when detecting reagent card, needs mobile reagent card itself, be easy to cause the precision of testing result Decline.
Due to the combination and marker material higher cost of antigen-antibody, it is aobvious that large area cannot be equally made into chemical reagent Color, and background has the impurity such as water, blood, marker material that infiltration is caused unevenly to increase the difficulty and precision of detection, how It keeps the volume of POCT product small, the stability detected and sensitivity are improved while easy to carry and improves efficiency, be this Technical field urgent problem to be solved.
Summary of the invention
To solve above-mentioned technical problem: the application proposes a kind of quasi- automated immunoassay analyzer, including reagent card conveying device 1, movable incubating device 3, detection device 4, the detection device 4 are in plane A, the movable incubating device 3 with Reagent card conveying device 1 is in same plane B, and plane A is located at the top of the plane B, the reagent card conveying device 1 It is incubated for for reagent card 2 to be successively transmitted to the movable incubating device 3,4 pairs of the detection device has been incubated for At reagent card 2 detected, be provided at least two parallel card slots on the movable incubating device 3, the incubation dress Setting 3 includes being incubated for detection platform 308, and the motion profile for being incubated for detection platform 308 is straight line, and the reagent card is defeated Send the direction of 1 delivery of therapeutic agents card 2 of device vertical with the incubation motion profile of detection platform 308.
The quasi- automated immunoassay analyzer, wherein further include device for discharging 5, the detection device 4 is filled with discharging It sets 5 and is in the plane A together and the two is arranged in parallel.
The quasi- automated immunoassay analyzer, wherein the device for discharging 5 includes the 5th motor 501, the 5th screw rod 502, the 5th screw rod mother set 503, discharging sliding block 504, the 5th sensing chip 505, the 5th proximal sensor 506, the 5th distal sensor Device 507, the 5th motor 501 control the movement of the 5th screw rod 502, drive the discharging sliding block 504 mobile, to carry out discharging behaviour Make, the 5th sensing chip 505 is coupled in the side of the discharging sliding block 504, cooperates the 5th proximal sensor 506, the Five distal sensors 507 are with the location information of the determination discharging sliding block 504.
The quasi- automated immunoassay analyzer, wherein it further include waste material box 6, the drive of device for discharging 5 incubation, The reagent card 2 detected is into the waste material box 6.
The quasi- automated immunoassay analyzer, wherein the reagent card conveying device 1 includes first support 101, first Motor 102, the first proximal sensor 103, the first screw rod mother set 104, the first screw rod 105, the first sensing chip 106, charging sliding block 107, feeding detection sensor 108, the first distal sensor 109, the first code reader 110, first code reader 110 are fixed on The positive upper end of reagent card 2 and close first distal sensor 109, the charging sliding block 107 is under the drive of the first screw rod 105 The movement of reagent card 2 is transmitted, the first motor 102 is fixed on close to the side of first proximal sensor 103, institute Feeding detection sensor 108 is stated for detection reagent card to determine that reagent card can carry out feeding operation, first sensing chip 106 It is fixed on the position that reagent card transmission is identified on the charging sliding block 107.
The quasi- automated immunoassay analyzer, wherein the movable incubating device 3 is same including third motor 301, third Step belt 302, third sensing chip 304, third support 305, third linear guide 306, third sliding block, is incubated at third inductor 303 Monitor station 308, temperature control electronic module 309 are educated, the third motor 301 drives the third synchronous belt 302, and the third is same Step belt 302 drives the third sliding block to move along the third linear guide 306, and the incubation monitor station 308 is fixedly installed It is snapped onto the third linear guide 306 above the third sliding block, while through the third sliding block.
The quasi- automated immunoassay analyzer, wherein the temperature control electronic module 309 is located at the incubation monitor station 308 Bottom surface, for controlling the temperature being incubated for.
The quasi- automated immunoassay analyzer, wherein the detection device 4 includes replaceable detector, the inspection Gauge head includes at least one fluorescence detection head, colloidal gold detector.
The detection device 4 include the 4th motor 401, the 4th screw rod 402, the 4th screw rod mother set 403, detector 404, Four-slider 405, the 4th linear guide 406, the 4th sensing chip 407, the 4th inductor 408, motor mounting plate 409, the 4th Seat 410, the detection device 4 detects the information of the immunofluorescence of the reagent card 2 by the detector 404, described Detector 404 is mobile by 406 tandem of the 4th linear guide, passes through the 4th sensing chip 407, the 4th inductor 408 determine the location information of detector 404.
A kind of detection method of quasi- automated immunoassay analyzer, includes the following steps:
(1) start analyzer, the reagent card for having added sample is placed in reagent card conveying device 1 by instrument startup self-detection On, pass through the corresponding information of the 110 identification agent card of code reader in reagent card conveying device 1 and is transmitted to system control dress It sets;
(2) reagent card is carried out being transmitted to movably for straight line along reagent card length direction by the reagent card conveying device 1 It is fixed on the incubation monitor station 308 of incubating device 3;
(3) according to the temperature requirements of each reagent card, temperature is carried out by the temperature control electronic module in movable incubating device 3 Degree control, and be incubated for according to the different reaction time;
(4) 4 motion detection head 404 of detection device moves forward, and carries out to the reagent card for reaching defined incubation time Detection, and result is fed back into system control device, after having detected, detector moves backward reset;
(5) discharging after the completion of detection, carries out discharging operations.
The detection method of the quasi- automated immunoassay analyzer, wherein specifically included in the step 1: from reagent card Reagent card is placed in 1 inlet of conveying device, and the first code reader 110 reads the information for the reagent card placed, and judges that information is It is no correct, if incorrect, alert, if correctly, carrying out reagent card conveying operations.
The detection method of the quasi- automated immunoassay analyzer, wherein specifically included in the step 3: the temperature control Electronic module 309 the bottom of the incubation monitor station 308 of movable incubating device 3 with adjust reagent snap into demand at a temperature of, The detection device 4 receives system control device for the incubation time information of each reagent card.
The detection method of the quasi- automated immunoassay analyzer, wherein the step 5 specifically includes: unloading described in control Expect that the 5th motor 501 in device 5 controls the movement of the 5th screw rod 502, drives the discharging sliding block 504 mobile, after will test Reagent card be dumped into waste material box 6.
The structure of the application is simple, compact, occupies little space, and greatly reduces cost;Multiple reagents can be controlled simultaneously It is reacted, and simultaneous reactions can be carried out for the control that different types of reagent is distinguished, improve detection effect Rate, it is convenient to operate, and reduces requirement to the professional standards of tester, easy to carry;Reagent card is fixed, detection device center Heart detector can be detected along guide rail linear movement, be can reach higher detection accuracy compared with other mobile reagent cards, passed through Mobile detector, keeps the stabilization of reagent card, and reducing reagent card movement causes the variations such as reaction environment, temperature to cause reaction result There is error;Each component mutual cooperation, co-ordination under control system control, realizes instrument intelligent prompt and false alarm, Further improve the degree of automation and performance of the analyzer.
Incubation reaction platform is set in the present invention mode of linear movement, the side of the disk of structure compared with the existing technology Formula is simpler, meanwhile, it is arranged between card slot by closely parallel mode, middle disk blocks at radial setting compared with the prior art The obvious volume of the mode of slot is much smaller, keeps analyzer of the invention easy to carry, and the application is skillfully constructed by multiple structures, Keep detection accuracy higher, using effect is prominent.
Detailed description of the invention
Automated immunoassay analyzer schematic diagram of internal structure subject to Fig. 1;
Fig. 2 is reagent card conveying device structural schematic diagram;
Fig. 3 is movable incubating device structural schematic diagram;
Fig. 4 is structure of the detecting device schematic diagram;
Fig. 5 is device for discharging structural schematic diagram.
Specific embodiment
The application is described in further detail with reference to the accompanying drawing, it is necessary to it is indicated herein to be, implement in detail below Mode is served only for that the application is further detailed, and should not be understood as the limitation to the application protection scope, the field Technical staff can make some nonessential modifications and adaptations to the application according to above-mentioned application content.
Embodiment one:
As shown in Figure 1, propose a kind of quasi- automated immunoassay analyzer for the application, including it is reagent card conveying device 1, movable Incubating device 3, detection device 4, device for discharging 5, waste material box 6, the detection device 4 at device for discharging 5 In same plane A and the two is arranged in parallel, and the movable incubating device 3 is in same plane B with reagent card conveying device 1 And be mutually perpendicular, plane A is located at the upper end of the plane B, and the reagent card conveying device 1 is elongated, for according to It is secondary reagent card 2 is transmitted to the movable incubating device 3 along the length direction of reagent card 2 to be incubated for, the discharging Device 5 drives the reagent card 2 being incubated for into the waste material box 6, it should be noted that, waste material box 6 can be wrapped necessarily Include, only illustrate in the example shown, device for discharging 5 can also discharging in other designated positions.
Be illustrated in figure 2 reagent card conveying device structural schematic diagram, the reagent card conveying device 1 include bracket 101, Motor 102, proximal sensor 103, screw rod mother set 104, screw rod 105, sensing chip 106, charging sliding block 107, feeding detection sensing Device 108, distal sensor 109, code reader 110, the code reader 110 are fixed on the positive upper end of reagent card 2 and close to distal ends Sensor 109, the charging sliding block 107 transmit the movement of reagent card 2 under the drive of screw rod 105, and the motor 102 is solid It is scheduled on close to the side of the proximal sensor 103, the feeding detection sensor 108 is for detection reagent card to determine whether Feeding operation can be carried out, the sensing chip 106 is fixed on the position that reagent card transmission is identified on the charging sliding block 107, institute State code reader 110 read each be put into the information of the reagent card conveying device 1, be transferred in system control device into Row judges whether information is correct, such as whether reagent card is face-up, if information is incorrect or relevant information is not detected, It then reports an error, and by alarm sounds operator, if correctly, system control device is according to relevant information, such as reaction time, instead Project is answered, reaction temperature etc. carries out subsequent control, such as detector is detected in the stipulated time.
As Fig. 3 show movable incubating device structural schematic diagram, wherein the movable incubating device 3 includes motor 301, synchronous belt 302, inductor 303, sensing chip 304, support 305, linear guide 306, sliding block (not identified in figure), incubate Monitor station 308, temperature control electronic module 309 are educated, the motor 301 drives the synchronous belt 302,302 band of synchronous belt It moves the sliding block to move along the linear guide 306, the incubation monitor station 308 is fixed above the sliding block, simultaneously It is snapped onto the linear guide 306 by the sliding block, the temperature control electronic module 309 is located at the incubation monitor station 308 Bottom, to need to carry out temperature adjusting according to reagent card, the inductor 303, sensing chip 304 are incubated for monitor station for sensing 308 location information in linear guide.
It is illustrated in figure 4 structure of the detecting device schematic diagram, wherein the detection device 4 includes motor 401, screw rod 402, screw rod mother set 403, detector 404, sliding block 405, linear guide 406, sensing chip 407, inductor 408, motor mounting plate 409, support 410, the detection device 4 detect the state of the reagent card 2, the detection by the detector 404 First 404 is mobile by 406 tandem of linear guide, determines detector 404 by the sensing chip 407, inductor 408 Location information, the motor 401 rotates, and driving screw rod 402 rotates, and pushes the detector 404 along the linear guide 406 is mobile, and the sensing chip 407 keeps the position consistency of relative rectilinear guide rail 406 with the detector 404, passes through inductor 408 sense 407 positions of sensing chip to judge the position of the detector 404.The detector is quantitative fluorescence analysis detector, For carrying out fluorescence detection to reagent card.
It is illustrated in figure 5 device for discharging structural schematic diagram, wherein the device for discharging 5 includes motor 501, screw rod 502, screw rod mother set 503, discharging sliding block 504, sensing chip 505, proximal sensor 506, distal sensor 507, the motor 501 It controls screw rod 502 to act, drives the discharging sliding block 504 mobile, to carry out discharging operations, the sensing chip 505 is coupled in institute The side for stating discharging sliding block 504 cooperates the proximal sensor 506, distal sensor 507 with the determination discharging sliding block 504 Location information.
A kind of detection method of quasi- automated immunoassay analyzer, wherein include the following steps:
The reagent card for having added sample is placed in reagent card conveying device 1 by 1 starting analyzer, instrument startup self-detection On, pass through the corresponding information of the 110 identification agent card of code reader in reagent card conveying device 1 and is transmitted to system control dress It sets;
Reagent card is carried out being transmitted to movably for straight line along reagent card length direction by the 2 reagent card conveying devices 1 It is fixed on the incubation monitor station 308 of incubating device 3;
3 according to the temperature requirements of each reagent card, and the temperature control electronic module passed through in movable incubating device 3 carries out temperature Degree control, and be incubated for according to the different reaction time;
4 detection device, 4 motion detection head 404 moves forward, and carries out to the reagent card for reaching defined incubation time Detection, and result is fed back into system control device, after having detected, detector moves backward reset;
5 dischargings after the completion of detection, carry out discharging operations.
The detection method of the quasi- automated immunoassay analyzer, wherein specifically included in the step 1: from reagent card Reagent card is placed in 1 inlet of conveying device, and just whether code reader 110 reads the information for the reagent card placed, and judge information Really, if it is incorrect, alert, if correctly, carrying out reagent card conveying operations.
The detection method of the quasi- automated immunoassay analyzer, wherein specifically included in the step 3: the temperature control Electronic module 309 the bottom of the incubation monitor station 308 of movable incubating device 3 with adjust reagent snap into demand at a temperature of, The detection device 4 receives system control device for the incubation time information of each reagent card.
The detection method of the quasi- automated immunoassay analyzer, wherein the step 5 specifically includes: unloading described in control Expect that the motor 501 in device 5 controls screw rod 502 and acts, drives the discharging sliding block 504 mobile, the reagent card after will test It is dumped into waste material box 6.
Embodiment two:
Detection device 4 includes detector, and the detector can be replaced manually, and colloidal gold detection can be selected in the detector Head, for carrying out colloidal gold detection, other structures or component are identical as fluorescence detection device.
The structure of the application is simple, compact, occupies little space, and greatly reduces cost;Multiple reagents can be controlled simultaneously It is reacted, and simultaneous reactions can be carried out for the control that different types of reagent is distinguished, improve detection effect Rate, it is convenient to operate, and reduces requirement to the professional standards of tester, easy to carry;Reagent card is fixed, detection device center Heart detector can be detected along guide rail linear movement, be can reach higher detection accuracy compared with other mobile reagent cards, passed through Mobile detector, keeps the stabilization of reagent card, and reducing reagent card movement causes the variations such as reaction environment, temperature to cause reaction result There is error;Each component mutual cooperation, co-ordination under control system control, realizes instrument intelligent prompt and false alarm, Further improve the degree of automation and performance of the analyzer.
Incubation reaction platform is set in the present invention mode of linear movement, the side of the disk of structure compared with the existing technology Formula is simpler, meanwhile, it is arranged between card slot by closely parallel mode, middle disk blocks at radial setting compared with the prior art The obvious volume of the mode of slot is much smaller, keeps analyzer of the invention easy to carry, and the application is skillfully constructed by multiple structures, Keep detection accuracy higher, using effect is prominent.

Claims (13)

1. a kind of quasi- automated immunoassay analyzer, it is characterised in that: including reagent card conveying device (1), movable incubating device (3), Detection device (4), the detection device (4) are in plane A, at the movable incubating device (3) and reagent card conveying device (1) It is located at the top of the plane B in same plane B, plane A, the reagent card conveying device (1) is used for successively by reagent card (2) It is transmitted to the incubating device (3) to be incubated for, the detection device (4) is detected to the reagent card (2) completed is incubated for, institute It states and is provided at least two parallel card slots on movable incubating device (3), the movable incubating device (3) includes being incubated for detection platform (308), the motion profile for being incubated for detection platform (308) is straight line, reagent card conveying device (1) the delivery of therapeutic agents card (2) direction is vertical with the incubation motion profile of detection platform (308), and reagent card can pass through the movable incubating device (3) the temperature control electronic module in carries out temperature control;The detection device (4) includes motion detection head (404), the movement inspection Gauge head (404) moves forward, and detects to the reagent card for reaching defined incubation time, and result is fed back to system control Device, after having detected, detector moves backward reset, by mobile detector, keeps the stabilization of reagent card.
2. quasi- automated immunoassay analyzer as described in claim 1, it is characterised in that: further include device for discharging (5), the detection Device (4) is in the plane A together with device for discharging (5) and the two is arranged in parallel.
3. quasi- automated immunoassay analyzer as claimed in claim 2, it is characterised in that: the device for discharging (5) includes the 5th electricity Machine (501), the 5th screw rod (502), the 5th screw rod mother set (503), discharging sliding block (504), the 5th sensing chip (505), the 5th are closely End sensor (506), the 5th distal sensor (507), the 5th motor (501) control the movement of the 5th screw rod (502), drive The discharging sliding block (504) is mobile, and to carry out discharging operations, the 5th sensing chip (505) is coupled in the discharging sliding block (504) side cooperates the 5th proximal sensor (506), the 5th distal sensor (507) with the determination discharging sliding block (504) location information.
4. quasi- automated immunoassay analyzer as claimed in claim 2, it is characterised in that: it further include waste material box (6), the discharging dress It sets (5) and drives and be incubated for, in the reagent card (2) that has detected to the waste material box (6).
5. quasi- automated immunoassay analyzer as described in claim 1, it is characterised in that: the reagent card conveying device (1) includes First support (101), first motor (102), the first proximal sensor (103), the first screw rod mother set (104), the first screw rod (105), the first sensing chip (106), charging sliding block (107), feeding detection sensor (108), the first distal sensor (109), First code reader (110), first code reader (110) are fixed on the positive upper end of reagent card (2) and close to the first distal sensors Device (109), the charging sliding block (107) transmitted under the drive of the first screw rod (105) reagent card (2) it is mobile, described the One motor (102) is fixed on close to the side of first proximal sensor (103), and the feeding detection sensor (108) is used In detection reagent card to determine that reagent card can carry out feeding operation, first sensing chip (106) is fixed on the charging sliding block (107) position of reagent card transmission is identified on.
6. quasi- automated immunoassay analyzer as described in claim 1, it is characterised in that: the movable incubating device (3) includes the Three motors (301), third synchronous belt (302), third inductor (303), third sensing chip (304), third support (305), Third linear guide (306), is incubated for monitor station (308), temperature control electronic module (309), the third motor at third sliding block (301) the third synchronous belt (302) is driven, the third synchronous belt (302) drives the third sliding block along described the Three linear guides (306) are mobile, and the incubation monitor station (308) is fixed above the third sliding block, and by described Third sliding block snaps on the third linear guide (306).
7. quasi- automated immunoassay analyzer as claimed in claim 6, it is characterised in that: the temperature control electronic module (309) is located at Incubation monitor station (308) bottom surface, for controlling the temperature being incubated for.
8. quasi- automated immunoassay analyzer as described in claim 1, it is characterised in that: the detection device (4) includes replaceable Detector, the detector includes at least one fluorescence detection head, colloidal gold detector.
9. quasi- automated immunoassay analyzer as claimed in claim 8, it is characterised in that: the detection device (4) further includes the 4th Motor (401), the 4th screw rod (402), the 4th screw rod mother set (403), detector (404), Four-slider (405), the 4th straight line Guide rail (406), the 4th sensing chip (407), the 4th inductor (408), motor mounting plate (409), the 4th support (410), it is described Detection device (4) detects the immunofluorescence of the reagent card (2) or the information of colloidal gold by the detector (404), described Detector (404) is mobile by the 4th linear guide (406) tandem, passes through the 4th sensing chip (407), the 4th Inductor (408) determines the location information of detector (404).
10. a kind of detection method of quasi- automated immunoassay analyzer, which comprises the steps of:
(1) starting analyzer, the reagent card for having added sample is placed on reagent card conveying device (1) by instrument startup self-detection, Pass through the corresponding information of the first code reader (110) identification agent card on reagent card conveying device (1) and is transmitted to system control Device;
(2) reagent card is transmitted to movable incubation along reagent card length direction progress straight line by the reagent card conveying device (1) It is fixed on the incubation monitor station (308) of device (3);
(3) according to the temperature requirements of each reagent card, temperature control is carried out by the temperature control electronic module in movable incubating device (3) System, and be incubated for according to the different reaction time;
(4) detection device (4) motion detection head (404) moves forward, and examines to the reagent card for reaching defined incubation time It surveys, and result is fed back into system control device, after having detected, detector moves backward reset, by mobile detector, keeps The stabilization of reagent card;
(5) discharging after the completion of detection, carries out discharging operations.
11. the detection method of quasi- automated immunoassay analyzer as claimed in claim 10, it is characterised in that: in the step (1) It specifically includes: placing reagent card from reagent card conveying device (1) inlet, the first code reader (110) reads the reagent card placed Information, and judge whether information correct, if incorrect, alert, if correctly, carrying out reagent card conveying Operation.
12. the detection method of quasi- automated immunoassay analyzer as claimed in claim 10, it is characterised in that: in the step (3) Specifically include: the temperature control electronic module (309) is in the bottom of the incubation monitor station (308) of movable incubating device (3) to adjust Reagent snaps at a temperature of demand, and the detection device (4) receives system control device for the incubation time of each reagent card Information.
13. the detection method of quasi- automated immunoassay analyzer as claimed in claim 10, it is characterised in that: step (5) tool Body includes: that the 5th motor (501) in the control device for discharging (5) controls the movement of the 5th screw rod (502), drives the discharging Sliding block (504) is mobile, and the reagent card after will test is dumped into waste material box (6).
CN201710064718.0A 2017-02-05 2017-02-05 A kind of quasi- automated immunoassay analyzer and detection method Active CN106706937B (en)

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