CN102512547B - Traditional Chinese medicine composition for treating canine distemper virus and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating canine distemper virus and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for treating canine distemper virus. The composition is prepared from pure natural herbs such as cordate houttuynia, honeysuckle, fructus forsythia, kawazure, radix sophorae flavescentis, cyrtomium fortune, Chinese violet, common andrographis herb, root of Chinese pulsatilla, radix isatidis and rhizoma alismatis. The invention further relates to a preparation method of the composition and use of the traditional Chinese medicine composition for preparation of the drug for treating the canine distemper virus. The product, disclosed by the invention, has remarkable treatment effect to early, middle and later stages of cases of the canine distemper virus.
Description
Technical field
The invention belongs to the veterinary medicine field, specifically, is a kind of Chinese medicine composition that is used for the treatment of canine distemper virus and its production and use, also relates to the application of said composition in the bad medicine for the treatment of canine distemper virus.
Background technology
In recent years, living standards of the people significantly improve, and population steps into aging society, and it is a kind of happy that the elderly keeps a pet, and house pet is a member in family, no matter are that the people that city or rural area keep a pet gets more and more.Particularly after China's entry into the World Trade Organization, the house pet of introducing from countries in the world is various in style, have from German shepherd, Australian Shepherd, English Springer Spaniel, Scotland shepherd dog, the U.S. can card, Borzoi, bar brother dog, Canis lupus familiaris, large red to Budgerigar, little have more than 80 to plant famous and precious Canis familiaris L. to the deer Canis familiaris L., also have more than 20 kind of local various stupid Canis familiaris L.s, separately have famous and precious cat more than 30 to plant.Streets and lanes and residential quarter in the city are found everywhere house pet miscellaneous, unavoidably allow the pedestrian see several more.A lot of house pets are played together and are easy to the cross infection various diseases, the diseases such as canine distemper disease as extremely strong in infectiousness, canine parvovirus disease, bacterial enteritis, dysentery, dysentery, viral transmissible gastroenteritis, canine distemper disease particularly, canine parvovirus disease is dog and other house pets are multiple, deadly infectious disease, be difficult to cure, mortality rate accounts for more than 98%.
Summary of the invention
An object of the present invention is to provide a kind of Chinese medicine composition for the treatment of canine distemper virus, said composition is prepared from by the raw material of Chinese medicine of following weight proportion:
Preferably, said composition is prepared from by the raw material of Chinese medicine of following weight proportion:
Chinese medicine composition of the present invention can be made various types of pharmaceutical preparatioies according to actual needs, for example makes injection, and is preferred but be not limited to injection.
Another object of the present invention is to provide a kind of preparation method for the treatment of the canine distemper virus compositions, it is characterized in that: comprise the following steps:
1, with Herba Houttuyniae, Flos Lonicerae, Fructus Forsythiae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Rhizoma Osmundae, Herba Violae, Herba Andrographis, the Radix Pulsatillae, Radix Isatidis, the dried medicine raw materials of Rhizoma Alismatis 11 flavor Chinese medicine clean, clean, drying, weigh by weight, add multi-function extractor, add the dried quality of medicinal material 8-12 of Chinese medicine purified water doubly, and be heated to boil, decocted 1-3 hour, medicinal liquid is leached, get filtrate for the first time, standby;
2, decocting for Chinese herbal medicine for the second time in multi-function extractor, adds the dried quality of medicinal material 6-10 of Chinese medicine purified water doubly, and is heated to boil, and decocts 1-2 hour, and medicinal liquid is leached, and filtrate, standby for the second time;
3, decocting for Chinese herbal medicine for the third time in multi-function extractor, adds the dried quality of medicinal material 4-6 of Chinese medicine purified water doubly, and is heated to boil, and decocts 1-2 hour, and medicinal liquid is leached, and filtrate, standby for the third time;
4, with step 1, step 2, step 3 standby for the first time, for the second time, filtrate is placed in container and mixes for the third time, precipitation is filtered, and gets filtrate, and is standby;
5, step 4 gained filtrate is concentrated with the Chinese medicinal steaming hair device, concentrated concentration is 2/5ths of filtrate, gets concentrated solution, and is standby;
6, step 5 gained concentrated solution is added the ethanol of 1 times of amount content 95%, carried out precipitate with ethanol 50-90 hour, then with separation of ethanol, get fluid extract for the first time; Add the ethanol of 0.8 times of amount content 95% to carry out precipitate with ethanol 30-80 hour fluid extract for the first time, separating alcohol then, fluid extract for the second time; Ethanol precipitate with ethanol 10-50 hour that fluid extract is for the second time added 0.7 times of amount content 95%, and then separation of ethanol, the crude drug extractum for preparing is standby;
7, press the standard preparation of Chinese veterinary pharmacopoeia, the crude drug extractum for preparing is adjusted to pH value 4.6～6.0, with the stock solution that configures, sterilization, packing namely gets the injection for the treatment of canine distemper virus.
Above-mentioned raw material and equipment is commercially available arriving all.
Need to prove, method of the present invention is a kind of preferred preparation technology but is not limited to this.Product by the inventive method preparation has good stability.
For many years, adopt Chinese medicine composition of the present invention, the treatment canine distemper virus and take diarrhoea, enteritis as the routine effect of gastroenteropathy incomplete statistics more than 30,000 of feature remarkable, cure rate is more than 99%.
Chinese medicine composition of the present invention is to canine distemper virus, Canine Parvovirus, Feline Panleukopenia Virus, hundstaupe exanthema virus, dog hemorrhagic intestinal class malignant bacteria, dog dust Li Shi body (rickettsia) pasteurellosis bacillus, and the pathogen such as streptococcus have very strong inhibition or killing action.Use the preparation of this Chinese medicine composition, can activate the activity of choline acetylase and inhibition choline enzyme, conditioning choline enzyme nervous system; digestive system, the function of cardiovascular system strengthens gastrointestinal tract to fat; protein, the absorption of sugar and moisture content, and very strong astringing to arrest diarrhea effect is arranged.The prescription compages of this medicine is according to traditional Chinese medical science monarch, minister, the formulation principle scientific composition helping, make, produce after each medicine forms very strong collaborative or shape the antibacterial action of making a concerted effort, can improve the curative effect decades of times.
Pharmacological action: the present invention is pure Chinese medicinal preparation, is efficient specially good effect, specially controls canine distemper, dog, and this medicine is to canine distemper, and Canine Parvovirus has strong inhibition or killing action.This product is to canine distemper, " canine parvovirus disease " early, in, evening, its case had good therapeutic effect ".
Indication: sick dog spirit is depressed, and appetite is absolutely useless, and fervescence is vomitted more than 40 ℃, and ophthalmic has a large amount of viscosity, the purulence eye corner of the eyes, asoscope is dry, walks haltingly on foot, the atrophy of eyeball after 48 hours of falling ill, skin elasticity descends, and has loose bowels, and diarrhoea is serious, the serious dehydration phenomenon occurs.Nervous symptoms appears in the sick dog that has, and namely warm thing is insane swollen, and diarrhoea appears in tic time quick property outbreak then, has blood in stool, and is brick-red, color of soy sauce, and cerise does not wait.This seasonal disease dog can not be stood, and in time injects the sinister pestilence spirit of dog injection, can in time alleviate the disease disease.The sinister pestilence spirit of dog injection is the specific drug of a kind of not controlling " Canine Parvovirus " severe disease, early, in, late period, sick dog was with this medicine cure rate a hundred per cent.Later stage sick dog appearance dehydration takes the condition of illness such as poisoning, heart failure, join Energy mixture, vitamin C, and fluid infusion, heart tonifying, toxin expelling strengthens the Comprehensive Treatments such as body immunity, and cure rate can reach 99 percent.
The functional component for the treatment of canine distemper virus Chinese medicine composition of the present invention is Herba Houttuyniae, Flos Lonicerae, Fructus Forsythiae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Rhizoma Osmundae, Herba Violae, Herba Andrographis, the Radix Pulsatillae, Radix Isatidis, Rhizoma Alismatis.
In the prescription for the treatment of canine distemper virus Chinese medicine composition of the present invention: put down in writing according to the Chinese medicine voluminous dictionary:
Herba Houttuyniae-[function cures mainly]: heat-clearing and toxic substances removing, inducing diuresis to remove edema.Control pneumonia, the dense infections of lung, hematodiarrhoea, malaria, edema, gonorrhea, leucorrhea, carbuncle, hemorrhoid, proctoptosis, eczema, tinea alba, scabies.
Flos Lonicerae-[function cures mainly]: heat clearing away, detoxifcation.Control the epidemic febrile disease heating, toxic-heat and blood stasis, carbuncle and ulcer, toxic swelling, scrofula, anal fistula.
Herba Violae-[function cures mainly]: clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling.Control furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhoea, conjunctival congestion, sore throat, venom.
Radix Isatidis-[function cures mainly]: heat clearing away, detoxifcation, removing heat from blood.Control influenza, epidemic encephalitis, encephalitis b, pneumonia, erysipelas, temperature is sent out speckle, and coma is told nosebleed, pharyngeal swelling, mumps, acute catarrhal conjunctivitis, skin ulcer rash.
Fructus Forsythiae-[function cures mainly]: heat clearing away, detoxifcation, eliminating stagnation, detumescence.Control warm, erysipelas, macule, carbuncle toxic swelling, scrofula, urine stranguria with urinary obstruction.
Herba Andrographis-[function cures mainly]: heat-clearing and toxic substances removing, removing heat from blood detumescence.Control acute bacillary dysentery, gastroenteritis, flu, epidemic encephalitis, tracheitis, pneumonia, pertussis, pulmonary tuberculosis, the dense infections of lung, cholecystitis, hypertension, epistaxis, oropharynx swells and ache, furuncle carbuncle, burn due to hot liquid or fire, venom.
Radix Sophorae Flavescentis-[function cures mainly]: heat clearing away, dampness, parasite killing.Control toxic-heat and blood stasis, discharging fresh blood stool, jaundice, leucorrhea with red and white discharge, infantile pneumonia, infantile malnutrition, acute tonsillitis, anal fistula, proctoptosis, skin pruritus, furuncle leprosy malignant boil, erosion of vulva pruritus due to damp pathogen, scrofula is scalded.
Rhizoma Osmundae-[function cures mainly]: kill ascarid, silk ribbon, pinworm, heat clearing away, detoxifcation, removing heat from blood, hemostasis.Control a minute hotness and emit, warm skin eruption is spitted blood, epistaxis, discharging fresh blood stool, dysentery, metrorrhagia, leukorrhagia.
The Radix Pulsatillae-[function cures mainly] clearing away heat and cooling blood, detoxifcation.Control toxic-heat and blood stasis, pyrexial malaria cold and heat, epistaxis, internal hemorrhoid with bleeding.
Rhizoma Coptidis-[function cures mainly]: heat clearing and damp drying, eliminating fire and detoxication; Damp-heat dysentery, hyperactivity of heart-fire, excessiveness of stomach-fire, dampness-heat in the liver and gallbladder, conjunctival congestion and swelling pain, fire-toxin carbuncle sore.The red skin of fish, the Bai Guanbai mouth is hemorrhage.
Rhizoma Alismatis-[function cures mainly]: diuretic, eliminating dampness by diuresis expels the heat-evil.Control dysuria, edema distension, vomiting, dysentery, phlegm retention, beriberi, gonorrhea, hematuria.
With the medical material in above-mentioned prescription by weight ratio complex group be fitted together, have following effect:
1, pure Chinese medicinal preparation, pure green medicine, combination is novel, science, rationally, medicine is pollution-free, and noresidue has no side effect.Human body is had no effect.
2, curative effect is outstanding, and effect is remarkable, and dosage is few, and cost is low, the short treating period instant effect, and canine distemper, Canine Parvovirus cures 99%.
3, this prescription is new and effective antibiotic, antiviral drug, and it has antibiotic, and chemicals is without the advantage of desirableization.
4, this prescription can be eliminated rapidly the sinister element of intestinal and the endotoxin in pet body, improves body's immunity.
5, participate in reconciling the body biochemical reaction, reconcile nerve, cardiovascular, the function of digestive system.
6, cardioprotection strengthens the gastrointestinal absorbability, and very strong receipts wound anti-diarrhea effect is arranged, and lasting medicine is stable.
For this reason, another object of the present invention is to provide the application of a kind of present composition in preparation treatment canine distemper disease cytotoxic drug.
The specific embodiment
Embodiment 1
Injection formulation (ProductName: dog poison pestilence spirit injection)
Raw material of Chinese medicine and consumption:
Herba Houttuyniae 2kg, Flos Lonicerae 1kg, Fructus Forsythiae 1kg, Rhizoma Coptidis 1kg, Radix Sophorae Flavescentis 1kg, Rhizoma Osmundae 1kg, Herba Violae 2kg, Herba Andrographis 2kg, Radix Pulsatillae 2kg, Radix Isatidis 2kg, Rhizoma Alismatis 1kg;
The preparation method concrete steps are as follows:
1) with Herba Houttuyniae, Flos Lonicerae, Fructus Forsythiae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Rhizoma Osmundae, Herba Violae, Herba Andrographis, the Radix Pulsatillae, Radix Isatidis, the Rhizoma Alismatis ten dried medicine raw materials of a herb clean, clean, dry, be ground into coarse powder, weigh by weight: Herba Houttuyniae 2kg, Flos Lonicerae 1kg, Fructus Forsythiae 1kg, Rhizoma Coptidis 1kg, Radix Sophorae Flavescentis 1kg, Rhizoma Osmundae 1kg, Herba Violae 2kg, Herba Andrographis 2kg, Radix Pulsatillae 2kg, Radix Isatidis 2kg, Rhizoma Alismatis 1kg adds in extraction pot, inject purified water 160kg, open the vacuum automatic switch, and be heated to boil, decocted 3 hours, medicinal liquid is leached, get filtrate for the first time, standby,
2) herb liquid extracts for the second time, in multi-function extractor, injects 128 kilograms of purified water, and is heated to boil, and decocts 2 hours, and medicinal liquid is leached, and gets filtrate for the second time, and is standby;
3) herb liquid extracts for the third time, in multi-function extractor, injects 90 kilograms of purified water, and is heated to boil, and decocts 1 hour, and medicinal liquid is leached, and gets filtrate for the third time, and is standby;
4) with step 1, step 2, step 3 standby for the first time, for the second time, filtrate is placed in container and mixes for the third time, precipitation is filtered, and gets filtrate, and is standby;
5) step 4 gained filtrate is concentrated with the Chinese medicinal steaming hair device, concentrated concentration is 2/5ths of filtrate, gets concentrated solution, and is standby;
6) step 5 gained concentrated solution is added the ethanol of 1 times of amount content 95%, carried out precipitate with ethanol 72 hours, then with separation of ethanol, get fluid extract for the first time; Add the ethanol of 0.8 times of amount content 95% to carry out precipitate with ethanol 48 hours fluid extract for the first time, separating alcohol then, fluid extract for the second time; The ethanol precipitate with ethanol 24 hours that fluid extract is for the second time added 0.7 times of amount content 95%, and then separation of ethanol, the crude drug extractum for preparing is standby.
7) press the standard preparation of Chinese veterinary pharmacopoeia, the crude drug extractum for preparing is adjusted to pH value 4.6～6.0, with the stock solution that configures, pour in the 5ml cillin bottle by production procedure in the workshop of ten thousand grades of GMP, sterilization again, packing, 6, every box is the injection that brown clear liquid namely gets the treatment canine distemper virus.
Usage and dosage: intramuscular injection or intravenous drip: per kilogram of body weight 0.1～0.4ml, every day 1～2 time, as intravenous drip suggestion 5% glucose injection, 150～250ml mixed diluting slowly instils and penetrates (consumption is decided by the domestic animal size).
Embodiment 2
Injection formulation (ProductName: dog poison pestilence spirit injection)
Raw material of Chinese medicine and consumption:
Herba Houttuyniae 3kg, Flos Lonicerae 0.5kg, Fructus Forsythiae 0.5kg, Rhizoma Coptidis 0.5kg, Radix Sophorae Flavescentis 0.5kg, Rhizoma Osmundae 0.5kg, Herba Violae 1kg, Herba Andrographis 1kg, Radix Pulsatillae 1kg, Radix Isatidis 1kg, Rhizoma Alismatis 0.5kg;
Preparation method is identical with the preparation method of embodiment 1
Embodiment 3
Injection formulation (ProductName: dog poison pestilence spirit injection)
Raw material of Chinese medicine and consumption:
Herba Houttuyniae 1kg, Flos Lonicerae 1.5kg, Fructus Forsythiae 1.5kg, Rhizoma Coptidis 1.5kg, Radix Sophorae Flavescentis 1.5kg, Rhizoma Osmundae 1.5kg, Herba Violae 3kg, Herba Andrographis 3kg, Radix Pulsatillae 3kg, Radix Isatidis 3kg, Rhizoma Alismatis 1.5kg;
Preparation method is identical with the preparation method of embodiment 1
Embodiment 4
Clinical trial
Clinical symptoms:
Sick dog spirit is depressed, and appetite is absolutely useless, and fervescence more than 40 ℃, vomiting, ophthalmic have that a large amount of viscosity, the purulence eye corner of the eyes, asoscope are dry, nervous symptoms appears in sick dog that have: i.e. the intermittent attacks such as spasm, epilepsy, tic.The appearance that continues diarrhoea, have blood in stool, brick-red, color of soy sauce, cerise do not wait, and serious dehydration phenomenon occurs afterwards in three days, atrophy of eyeball, and skin elasticity descends, and has pinched the skin gauffer and has disappeared slow.Because acidosis appears in dehydration, heart failure and death.
4 groups of trouble " canine distemper disease, canine parvovirus disease " are carried out the clinical experiment treatment, suffer from dog for 325 every group, therapeutic outcome sees the following form:
1, use " the injection formulation of embodiments of the invention 1 (ProductName: dog poison pestilence spirit injection) treatment " canine distemper disease ", " canine parvovirus disease " effect observation catalog (experimental group)
2, use " antibiotics, antiviral agents " treatment " canine distemper disease ", " canine parvovirus disease " effect observation catalog (matched group)
From the injection formulation of above-mentioned application embodiments of the invention 1 (ProductName: dog poison pestilence spirit injection) and application " antibiotics, antiviral agents " respectively two groups " canine distemper disease ", " canine parvovirus disease " are carried out randomized controlled treatment.Result of the test shows, (ProductName: dog poison pestilence spirit injection) capacity treatment " canine distemper disease ", " canine parvovirus disease " cure rate can reach 99% to use the injection formulation of embodiments of the invention 1.
Use " antibiotics, antiviral agents " and carry out randomized controlled treatment to two groups respectively.Result shows that cure rate is zero, can only be to have seldom a part of sick dog that slight improvement is arranged.Experimental results show that antibiotics, antiviral agents are treated " canine distemper disease ", " canine parvovirus disease " is invalid.
Above example of the present invention is had been described in detail, but described content is only preferred embodiments of the present invention, can not be considered to be used to limiting practical range of the present invention.All equalizations of doing according to the present patent application scope change and improve etc., within all should belonging to patent covering scope of the present invention.
Claims (4)
1. Chinese medicine composition for the treatment of canine distemper virus, said composition is prepared from by the raw material of Chinese medicine of following weight proportion:
Herba Houttuyniae: 10-30 Flos Lonicerae: 5-15 Fructus Forsythiae: 5-15
Rhizoma Coptidis: 5-15 Radix Sophorae Flavescentis: 5-15 Rhizoma Osmundae: 5-15
Herba Violae: 10-30 Rhizoma Alismatis: the 5-15 Radix Pulsatillae: 10-30
Radix Isatidis: 10-30 Herba Andrographis: 10-30.
2. a kind of Chinese medicine composition for the treatment of canine distemper virus according to claim 1 is characterized in that: said composition is that the raw material of Chinese medicine by following weight proportion is prepared from:
Herba Houttuyniae: 20 Flos Loniceraes: 10 Fructus Forsythiaes: 10
Rhizoma Coptidis 10 Radix Sophorae Flavescentiss: 10 Rhizoma Osmundae: 10
Herba Violae: 20 Rhizoma Alismatis: 10 Radix Pulsatillaes: 20
Radix Isatidis: 20 Herba Andrographis: 20.
According to claim 1 and 2 arbitrary group the treatment canine distemper virus Chinese medicine composition, it is characterized in that: described compositions is injection.
4. the preparation methoies of Chinese medicine composition of according to claim 1-3 described arbitrary group for the treatment of canine distemper virus is characterized in that: comprise the following steps:
1) with Herba Houttuyniae, Flos Lonicerae, Fructus Forsythiae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Rhizoma Osmundae, Herba Violae, Herba Andrographis, the Radix Pulsatillae, Radix Isatidis, the dried medicine raw materials of Rhizoma Alismatis 11 flavor Chinese medicine clean, clean, drying, weigh by weight, add multi-function extractor, add the dried quality of medicinal material 8-12 of Chinese medicine purified water doubly, and be heated to boil, decocted 1-3 hour, medicinal liquid is leached, get filtrate for the first time, standby;
2) decocting for Chinese herbal medicine for the second time in multi-function extractor, adds the dried quality of medicinal material 6-10 of Chinese medicine purified water doubly, and is heated to boil, and decocts 1-2 hour, and medicinal liquid is leached, and filtrate, standby for the second time;
3) decocting for Chinese herbal medicine for the third time in multi-function extractor, adds the dried quality of medicinal material 4-6 of Chinese medicine purified water doubly, and is heated to boil, and decocts 1-2 hour, and medicinal liquid is leached, and filtrate, standby for the third time;
4) with step 1, step 2, step 3 standby for the first time, for the second time, filtrate is placed in container and mixes for the third time, precipitation is filtered, and gets filtrate, and is standby;
5) step 4 gained filtrate is concentrated with the Chinese medicinal steaming hair device, concentrated concentration is 2/5ths of filtrate, gets concentrated solution, and is standby;
6) step 5 gained concentrated solution is added the ethanol of 1 times of amount content 95%, carried out precipitate with ethanol 50-90 hour, then with separation of ethanol, get fluid extract for the first time; Add the ethanol of 0.8 times of amount content 95% to carry out precipitate with ethanol 30-80 hour fluid extract for the first time, separating alcohol then, fluid extract for the second time; Ethanol precipitate with ethanol 10-50 hour that fluid extract is for the second time added 0.7 times of amount content 95%, and then separation of ethanol, the crude drug extractum for preparing is standby;
7) press the standard preparation of Chinese veterinary pharmacopoeia, the crude drug extractum for preparing is adjusted to pH value 4.6～6.0, with the stock solution that configures, then sterilization, packing namely gets the injection for the treatment of canine distemper virus.
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| CN103720929A (en) * | 2013-11-15 | 2014-04-16 | 北安市飞龙动物药厂 | Andrographis paniculata injection |
| CN103720948A (en) * | 2013-11-15 | 2014-04-16 | 北安市飞龙动物药厂 | Traditional Chinese medicine composition for treating canine distemper and canine parvovirus diseases |
| CN103720927A (en) * | 2013-11-15 | 2014-04-16 | 北安市飞龙动物药厂 | Traditional Chinese medicine preparation for treating dairy cow mastitis |
| CN105434941A (en) * | 2015-12-22 | 2016-03-30 | 王琪 | Traditional Chinese medicine composition treating skin type canine distemper |
| CN107456516A (en) * | 2017-06-28 | 2017-12-12 | 禹州市合同泰药业有限公司 | A kind of traditional Chinese herbal decoction of effectively treatment dog pest |
| ES2964618T3 (en) * | 2018-01-31 | 2024-04-08 | Sichuan Inlu Weite Pharmaceutical Tech Co Ltd | Saponin B4 from pulsatilla for the treatment of mastitis in dairy cows |
| CN112293576B (en) * | 2020-10-30 | 2023-11-24 | 四川九章生物科技有限公司 | Application of chlorogenic acid in preparation of medicines or feed additives for preventing and/or treating canine parvovirus diseases and canine distemper |
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