CN101407767A - Method for producing Chinese caterpillar fungus by fermentation - Google Patents
Method for producing Chinese caterpillar fungus by fermentation Download PDFInfo
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- CN101407767A CN101407767A CNA2008102288465A CN200810228846A CN101407767A CN 101407767 A CN101407767 A CN 101407767A CN A2008102288465 A CNA2008102288465 A CN A2008102288465A CN 200810228846 A CN200810228846 A CN 200810228846A CN 101407767 A CN101407767 A CN 101407767A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 20
- 230000004151 fermentation Effects 0.000 title claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 241000233866 Fungi Species 0.000 title description 4
- 240000001307 Myosotis scorpioides Species 0.000 title description 3
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 17
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 24
- 241000190633 Cordyceps Species 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 9
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 4
- 150000008065 acid anhydrides Chemical class 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 4
- 238000012262 fermentative production Methods 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 239000008176 lyophilized powder Substances 0.000 claims description 4
- 229910052717 sulfur Inorganic materials 0.000 claims description 4
- 239000011593 sulfur Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 abstract description 6
- 239000003814 drug Substances 0.000 abstract description 4
- 239000001963 growth medium Substances 0.000 abstract 3
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 239000002932 luster Substances 0.000 abstract 1
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 24
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 13
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 12
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- 229960005305 adenosine Drugs 0.000 description 12
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 12
- 239000000047 product Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000019156 vitamin B Nutrition 0.000 description 4
- 239000011720 vitamin B Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 150000001408 amides Chemical class 0.000 description 3
- 229940024606 amino acid Drugs 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 230000000144 pharmacologic effect Effects 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 150000003212 purines Chemical class 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 229940075420 xanthine Drugs 0.000 description 2
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 241001264174 Cordyceps militaris Species 0.000 description 1
- 241000130660 Hepialidae Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 241000634997 Leucon Species 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 241000330896 Thitarodes armoricanus Species 0.000 description 1
- 102000030621 adenylate cyclase Human genes 0.000 description 1
- 108060000200 adenylate cyclase Proteins 0.000 description 1
- -1 alkyl silica gel Chemical compound 0.000 description 1
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- 230000003115 biocidal effect Effects 0.000 description 1
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- 230000036983 biotransformation Effects 0.000 description 1
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- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- UQVDQSWZQXDUJB-UHFFFAOYSA-N hydron;7h-purin-6-amine;chloride Chemical compound Cl.NC1=NC=NC2=C1NC=N2 UQVDQSWZQXDUJB-UHFFFAOYSA-N 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
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- 210000003734 kidney Anatomy 0.000 description 1
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- 239000012982 microporous membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of medicine and provides a method for producing aweto by fermentation. The method adopts a MK722 culture medium, places an Erlenmeyer flask containing liquid seeds onto a rotary shaking table, and comprises the liquid seed cultivation that is characterized in that the proportion of the seeds to the culture medium is between 1 to 5 and 1 to 20, the rotation speed is 50 to 300 turns per minute, the cultivation time is 50 to 200 hours, the cultivation temperature is 15 to 30 DEG C, the lighting intensity is 0 to 58.4 Mu mol question mark m<-2> question mark s<-1>, and the lighting time lasts for 1 to 24 hours. After the liquid seeds are cultivated for 50 to 200 hours, the seeds with limpid color and luster, uniform mycelia and the diameter of about 1 to 3 mm in the seed liquid are selected for cultivation, and the selected seeds are inoculated into a bioreactor with the capacity of 0.2 to 5000 L according to the inoculation amount that is provided with the proportion of the liquid seeds to the culture medium between 1 to 1 and 1 to 20, and cultivated under aseptic condition. The cultivation temperature is 15 to 30 DEG C, the cultivation time is 50 to 96 hours, the rotation speed is 50 to 300 turns per minute, the lighting time lasts for 1 to 24 hours, and the lighting intensity is 0 to 58.4 Mu mol question mark m<-2> question mark s<-1>. The cultivated seeds are further freezing dried so as to form the frozen dry powder of fermented aweto. The method has easy operation and low production cost, does not pollute environment, and achieves the industrialization level.
Description
Technical field
The invention belongs to medical technical field, relate to a kind of method by fermentative production Cordyceps sinensis.Be means specifically with the microbial fermentation technology, cultivate and culture is carried out freeze dried method by the aweto in large scale that in the reciprocating type incubator of large-scale constant temperature illumination, carries out.
Background technology
Cordyceps sinensis is that Clavicipitaceae (Clavicepitaceae) fungi Cordyceps fungus (Cordycepssinensis (Berk.) Sacc.) colonizes in stroma and the larva cadaveric complex on Hepialidae insect bat moth (Hepialus armoricanus) larva.Have and invigorate the lung and the kidney, effects such as hemostasis and phlegm.Modern pharmacology shows that Cordyceps sinensis also has anticancer, antiviral, the anti-ageing effect of waiting for a long time.Contain multiple active substance with medicinal health care function in the Cordyceps bacterium, wherein adenosine (adenosine) and cordycepin (cordycepin) are one of main active substances.Cordyceps sinensis just extensively is used as invigorant among the people because of its pharmacologically active widely from ancient times, but strict and parasitic conditions is special because of its growing environment, adds the people for excavating transition, and the natural resource rareness costs an arm and a leg.Mainly concentrate on the determining of asexual shape, artificial culture, liquid submerged fermentation cultivation, chemical ingredients and Pharmacological action study about the research of Cordyceps sinensis in recent years.Through studies show that in a large number the artificial fermentation cultivates the mycelium that obtains, through researchs such as toxicity, pharmacology and plants, proof and natural cs chemical constitution, pharmacological action basically identical, and the content of some compositions is higher than natural cordyceps, and therefore people are devoted to submerged fermentation cultivation Cordyceps mycelium product as an alternative always for a long time.Adenosine and cordycepin are the main active substances of Cordyceps sinensis.Adenosine has the blood circulation of the heart and brain of improvement, prevents irregular pulse, suppresses neurotransmitter and discharges and regulate pharmacological actions such as adenylate cyclase activity, at present as the quality control index of Cordyceps sinensis.Cordycepin promptly 3 '-Desoxyadenosine, have immunomodulatory, antibiotic, antiviral, the anticancer and anti-ageing effect of waiting for a long time.
The research of at present domestic liquid culture about Cordyceps sinensis also is in the investigation of the various physical and chemical factors that carry out at different meta-bolitess basically, some scholars were applied to the liquid culture of Cordyceps sinensis with the research method of bio-transformation in recent years, but about investigating precursor substance the influence for secondary metabolite in the Cordyceps sinensis fermentation culture were also rarely had report.
The Cordyceps mycelium preparation that domestic and international Cordyceps sinensis class healthcare products that gone on the market and medicine are natural extract.Because it has very high pharmaceutical use, liked by the patient.Yet because its complicated component, active constituent content is low, has influenced the clinical efficacy of this medicine and the further expansion in market greatly.Particularly natural cordyceps has been subjected to the focused protection of the world and the Chinese government as endangered plant species, forbids to pluck.Each country all drops into the substitute products of lot of manpower and material resources research Cordyceps sinensis for this reason.The substitute products of generally acknowledging in the world are artificial culture Cordyceps sinensis or cordyceps militaris mycelium at present, and the high bacterial classification of screening active constituent content carries out artificial culture, and the mycelium that obtains carries out some conventional processing and makes medicinal extract, capsule or oral liquid.This still belongs to the traditional industry processing mode of Chinese medicine, and it is about 3 months that the production cycle is about.
Summary of the invention
The purpose of this invention is to provide a kind of method by fermentative production Cordyceps sinensis.
The present invention is achieved through the following technical solutions:
The present invention includes liquid seeds cultivation, large scale fermentation cultivation, freeze-drying.
The present invention is according to Cordyceps mycelium fermentation culture production technology, on the MK722 substratum, cultivate, the growth of bacterium ball and the output of effective constituent have been controlled well, the ratio 1 of seed and substratum: 5-20 during liquid seeds is cultivated, rotating speed: 50-300 rev/min, incubation time: 50-200 hour, culture temperature: 15-30 ℃, intensity of illumination: 0-58.4 μ molm
-2S
-1, light application time: 1-24 hour.
Liquid seeds and substratum ratio 1 during large scale fermentation is cultivated: the 1-20 inoculum size is connected in the 0.2-5000L bio-reactor culture temperature: 15-30 ℃, incubation time: 50-96 hour, rotating speed: 50-300 rev/min, light application time: 1-24 hour, intensity of illumination: 0-58.4 μ molm
-2S
-1
Freeze-drying process is sugared acid anhydride class 0.1-5mg/ml of adding and sulfur-containing amino acid 0.0001-12g/100g in fermented liquid ,-20--70 ℃ following freeze-drying, and then form the Cordyceps fermented product lyophilized powder.
Do not see that from domestic and international document of having delivered and patent utilization MK722 substratum and above-mentioned culture condition carry out the report that the Chinese caterpillar fungus technology is cultivated in submerged fermentation.
The culture process of Cordyceps sinensis is as follows:
One, liquid seeds is cultivated
1.MK722 substratum is formed:
Potato powder 1-4.75%, Semen Maydis powder 0.1-1.5%, peptone 0.1-0.5%, KH
2PO
40.1-0.5%, MgSO
40.1-0.5%, vitamins B
10.001-0.005%, vitamins B
20.001-0.005%, (NH
4)
2SO
40.01-0.10%, purine class 0.05-1%, amides 0.01-1%, all the other are sterile distilled water, the pH value is 5.0-7.0.
2. culture condition:
The Erlenmeyer flask that liquid seeds is housed is put on the rotary shaking table ratio 1 of seed and nutrient solution: 5-20, rotating speed: 50-300 rev/min, incubation time: 50-200 hour.Culture temperature: 15-30 ℃, intensity of illumination: 0-58.4 μ molm
-2S
-1, light application time: 1-24 hour.
Two, fermentation culture
Liquid seeds was cultivated after 50-200 hour, to cultivate when choosing limpid in sight, the mycelium uniformity, the about 1-3mm size of seed diameter in the seed liquor, in liquid seeds and substratum ratio 1: the 1-20 inoculum size, be connected in the 0.2-5000L bio-reactor, under aseptic condition, cultivate.
1. fermention medium is formed:
The same seed liquor substratum is formed.
2. culture condition:
Culture temperature: 15-30 ℃, incubation time: 50-96 hour, rotating speed: 50-300 rev/min, light application time: 1-24 hour, intensity of illumination: 0-58.4 μ molm
-2S
-1
3. check: mycelium uniformity, cordycepin content 0.05-2.6mg/g, adenosine content 0.001-2.0mg/g.
Three, freeze-drying
In fermented liquid, add sugared acid anhydride class 0.1-5mg/ml and sulfur-containing amino acid 0.0001-12g/100g ,-20--70 ℃ following freeze-drying, form Cordyceps sinensis fermentation lyophilized powder.
Four, detect
1. the selection of chromatographic condition:
Chromatographic condition: pump: SHIMADZU LC-10Avp; Detector: SHIMADZUSPD-10Avp; Chromatographic column: DIAMONSIL C-18 alkyl silica gel post (250 * 4.6); 5 μ l quantitatively encircle; Moving phase: acetonitrile: water: methyl alcohol (2: 85: 2); Measure wavelength: 260nm; Flow velocity: 1.0ml/min; Column temperature: 40 ℃.
2. the mensuration of sample:
Precision claims to conclude a contract or treaty the 0.5g lyophilized powder, puts in the ground Erlenmeyer flask, adds 20ml 90% methyl alcohol, refluxing extraction 30 minutes, suction filtration, residue is with 90% methanol wash 2 times, concentrate on the filtrate water bath, 90% methanol constant volume in the 5ml volumetric flask, 0.45 μ m filtering with microporous membrane.The gained trial-product reads peak area respectively at 0h, 2h, 4h, 7h sample introduction, calculates RSD.By substitution typical curve calculation sample cordycepin and adenosine content.
Produce Cordyceps sinensis with the inventive method fermentation culture technology, unchangeability, cost is low, and the cycle is short, the Chinese caterpillar fungus freeze-dried powder of formation, adenosine and cordycepin output height, active good, clinical effectiveness is obvious, has the market competitiveness.
Embodiment:
Embodiment 1: the preparation of Cordyceps sinensis
One, liquid seeds is cultivated
1.MK722 substratum is formed:
Potato powder 2%, Semen Maydis powder 0.8%, peptone 0.4%, KH
2PO
40.3%, MgSO
40.4%, vitamins B
10.003%, vitamins B
20.004%, (NH
4)
2SO
40.05%, purine class 0.65%, amides 0.2%, all the other are sterile distilled water, the pH value is 6.5.
2. culture condition:
The Erlenmeyer flask that liquid seeds is housed is put on the rotary shaking table, and the ratio of seed and nutrient solution 1: 20, was cultivated 100 hours by 100 rev/mins.Culture temperature: 20 ℃, intensity of illumination: 40.7 μ molm
-2S
-1Light application time: 4 hours.
Two, fermentation culture
Liquid seeds was cultivated after 100 hours, to cultivate when choosing limpid in sight, the mycelium uniformity, the about 1mm size of the seed diameter in the seed liquor is pressed 15% inoculum size, is connected in the 0.5L bio-reactor, cultivates under aseptic condition.
1. fermention medium is formed:
The same seed liquor substratum is formed.
2. culture condition:
Culture temperature: 20 ℃, cultivated 85 hours, 150 rev/mins of rotating speeds, light application time: 4 hours, intensity of illumination: 45.5 μ molm
-2S
-1
3. check:
The mycelium uniformity, cordycepin content 1.1mg/g, adenosine content 0.04mg/g.
Three, freeze-drying
In fermented liquid, add vehicle sugar acid anhydride class 3mg/ml and sulfur-containing amino acid 2g/100g,, form Cordyceps sinensis fermentation lyophilized powder-45 ℃ of following freeze-drying.
Embodiment 2: different purine are to the influence of adenosine and cordycepin output in the Cordyceps sinensis fermentation
1, xanthine;
| Xanthine | Cordycepin content (mg/g) | Adenosine content (mg/g) |
| (0.5％) | 1.72 | 0.05 |
2, VITAMIN B4
3, Leucon
4, guanine
Embodiment 3: different acid amides are to the influence of adenosine and cordycepin output in the Cordyceps sinensis fermentation
1, l-asparagine
2, glutamine
3, niacinamide
4, o-dime thyl phosphoroamidothioate
Embodiment 4: the comparison of adenosine and cordycepin output in 3 batches of samples of wild cordyceps and Cordyceps fermented product
Claims (5)
1. method by fermentative production Cordyceps sinensis, it is characterized in that: this method comprises that liquid seeds is cultivated, large scale fermentation is cultivated, freeze-drying.
2. method according to claim 1 is characterized in that: the ratio 1 of seed and substratum: 5-20 during liquid seeds is cultivated, and rotating speed 50-300 rev/min, cultivated 50-200 hour, culture temperature: 15-30 ℃, intensity of illumination: 0-58.4 μ molm
-2S
-1, light application time: 1-24 hour.
3. method according to claim 2 is characterized in that: described substratum is the MK722 substratum.
4. method according to claim 1, it is characterized in that: liquid seeds and substratum ratio 1 during described large scale fermentation is cultivated: 1-20 inoculum size, be connected in the 0.2-5000L bio-reactor, culture temperature: 15-30 ℃, cultivated 50-96 hour, rotating speed: 50-300 rev/min, light application time: 1-24 hour, intensity of illumination: 0-58.4 μ molm
-2S
-1
5. method according to claim 1 is characterized in that: freeze-drying process is sugared acid anhydride 0.1-5mg/ml of adding and sulfur-containing amino acid 0.0001-12g/100g in fermented liquid ,-20--70 ℃ following freeze-drying, and then form the Cordyceps fermented product lyophilized powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008102288465A CN101407767A (en) | 2008-11-17 | 2008-11-17 | Method for producing Chinese caterpillar fungus by fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008102288465A CN101407767A (en) | 2008-11-17 | 2008-11-17 | Method for producing Chinese caterpillar fungus by fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101407767A true CN101407767A (en) | 2009-04-15 |
Family
ID=40570981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008102288465A Pending CN101407767A (en) | 2008-11-17 | 2008-11-17 | Method for producing Chinese caterpillar fungus by fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101407767A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103211212A (en) * | 2013-04-11 | 2013-07-24 | 天津天狮生物发展有限公司 | Cordyceps mycelia and preparation method thereof |
| CN104904496A (en) * | 2015-06-18 | 2015-09-16 | 上海善力健生物科技有限公司 | Preparation method for cordyceps sinensis mycelium |
| CN105154491A (en) * | 2015-09-02 | 2015-12-16 | 天津现代职业技术学院 | Cordyceps sinensis liquid state fermentation culture medium for producing exopolysaccharides |
| CN106282028A (en) * | 2015-05-29 | 2017-01-04 | 东海大学 | Method for culturing cordyceps sinensis |
-
2008
- 2008-11-17 CN CNA2008102288465A patent/CN101407767A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103211212A (en) * | 2013-04-11 | 2013-07-24 | 天津天狮生物发展有限公司 | Cordyceps mycelia and preparation method thereof |
| CN103211212B (en) * | 2013-04-11 | 2016-01-20 | 天津天狮生物发展有限公司 | A kind of Cordyceps mycelium and preparation method |
| CN106282028A (en) * | 2015-05-29 | 2017-01-04 | 东海大学 | Method for culturing cordyceps sinensis |
| CN106282028B (en) * | 2015-05-29 | 2020-02-18 | 东海大学 | Method for culturing cordyceps sinensis |
| CN104904496A (en) * | 2015-06-18 | 2015-09-16 | 上海善力健生物科技有限公司 | Preparation method for cordyceps sinensis mycelium |
| CN105154491A (en) * | 2015-09-02 | 2015-12-16 | 天津现代职业技术学院 | Cordyceps sinensis liquid state fermentation culture medium for producing exopolysaccharides |
| CN105154491B (en) * | 2015-09-02 | 2018-08-24 | 天津现代职业技术学院 | A kind of culture medium for cordyceps sinensis liquid state fermentation production exocellular polysaccharide |
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