CN104904496A - Preparation method for cordyceps sinensis mycelium - Google Patents
Preparation method for cordyceps sinensis mycelium Download PDFInfo
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- CN104904496A CN104904496A CN201510341053.4A CN201510341053A CN104904496A CN 104904496 A CN104904496 A CN 104904496A CN 201510341053 A CN201510341053 A CN 201510341053A CN 104904496 A CN104904496 A CN 104904496A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention relates to a preparation method for a cordyceps sinensis mycelium. The preparation method for the cordyceps sinensis mycelium comprises the following steps that 1, a living worm of a swift moth family larva is beaten to be suspension; 2, the suspemnsion obtained in the step 1 is subjected to sterilization treatment to serve as a stand-by culture medium; 3, the culture medium obtained in the step 2 is placed into a fermentation tank, Hisutella sinensis bacteria and swift moth paecilomyces bainier bacteria are inoculated; 4, the fermentation condition of the fermentation tank is controlled, and the fermental cultivation is performed to obtain the cordyceps sinensis mycelium. Compared with the prior art, the preparation method for the cordyceps sinensis mycelium takes the swift moth family larva as the culture medium, the Hisutella sinensis bacteria and swift moth paecilomyces bainier bacteria are inoculated to be subjected to deep liquid fermentation, the component of the obtained cordyceps sinensis mycelium is highly similar to that of natural cordyceps sinensis, and the content is far higher than that of the cordyceps sinensis mycelium obtained by other fermentation methods.
Description
Technical field
The present invention relates to a kind of fermentation process, especially relate to a kind of preparation method of cordyceps mycelia.
Background technology
Cordyceps sinensis is the entomogenous fungi complex that Clavicipitaceae fungi autoeciousness is formed in Hepialidae larva body.Hirsutella sinensis and Paecilomyces hepiali chen two class fungi has been isolated in the research of Cordyceps sinensis.
The rare characteristic of preciousness due to Cordyceps sinensis, focus has been placed on and has reached acquisition active substance---" cordyceps mycelia " similar to natural cordyceps by cordyceps mycelia liquid mushroom development method by increasing scientific research institution and enterprise.
Present stage mainly with additional nutriment for the submerged fermentation of cordyceps mycelia, if glucose, exogenous proteins powder, dusty yeast, external source electrolyte are culture medium raw material, then is accessed corresponding fungi and ferments.This fermentation process ferments as bacterial classification though have employed Paecilomyces hepiali chen, but medium source is not the Hepialidae larva of Cordyceps sinensis itself, therefore the cordyceps mycelia ingredient of gained of fermenting can be distinguished to some extent with natural cs, does not possess effect of natural cs.
Chinese patent CN 102696392 A discloses a kind of method for increasing of natural cordyceps, and the specific practice of the method is as follows: (1) natural cordyceps increases the selection in base; (2) setting of collection lamp is lured; (3) configuration of liquid collecting is lured; (4) Screening of Media of Hirsutella hepiali Chen et Shen and peacilomyce hepiahi bacterium kind and preparation: the control of (5) volume increase condition.This invention utilizes high-pressure sodium lamp to carry out luring collection when the adult stage, make the bat noctuid adult of its periphery fly collection in Chinese caterpillar fungus base, increase egg laying amount.And configure ethanol, vinegar, liquid glucose spill on the grassy marshland being beneficial to larval growth, and its larva is collected at together.And in larva clustering area, spray Hirsutella hepiali Chen et Shen and Paecilomyces hepiali chen liquid spawn and increase the infection rate of bat exigua larvae, reaching with this object increasing cordyceps yield, thus realize industrialization and produce.
In this patent, medium adopts additional nutriment, if glucose, exogenous proteins powder, dusty yeast, external source electrolyte are culture medium raw material, access corresponding fungi again to ferment, the cordyceps mycelia ingredient of fermentation gained can be distinguished to some extent with natural cs, does not possess effect of natural cs.
Summary of the invention
Object of the present invention is exactly provide a kind of preparation method of cordyceps mycelia to overcome defect that above-mentioned prior art exists.
Object of the present invention can be achieved through the following technical solutions:
A preparation method for cordyceps mycelia, comprises the following steps:
(1) worm alive of Hepialidae larva is beaten into suspension;
(2) carry out sterilization treatment to step (1) gained suspension, temperature is 120-122 DEG C, and the time is 28-32 minute, and the suspension after sterilizing is stand-by as medium;
(3) step (2) gained medium is put into fermentation tank, inoculation Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind, wherein, the inoculum concentration of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is 5 ~ 8wt% of medium, and the weight ratio of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is 1:2 ~ 2:1, be preferably 1:1;
(4) control the fermentation condition of fermentation tank, fermentation tank throughput controls at 0.6-1.0m
3/ h, fermentation temperature 24 ~ 28 DEG C, fermentation time 106-112 hour, after fermentation, the centrifugal filtration of gained zymotic fluid obtains cordyceps mycelia, to cordyceps mycelia freeze drying, and obtains Chinese caterpillar fungus finished product mycelium powder after ultra-fine grinding.
The composition of Chinese caterpillar fungus finished product mycelium powder obtained by the present invention and the composition of commercially available cordyceps mycelia powder contrast as following table:
Can find out, the Chinese caterpillar fungus finished product mycelium powder main composition content that preparation method of the present invention obtains is far above existing commercially available prod.
Compared with prior art, the present invention is for medium with Hepialidae larva, its growing environment closer to natural cs and nutrition foundation, and other medium are all artificial preparation, access Hirsutella sinensis and peacilomyce hepiahi bacterium kind carry out deep fermentation, the cordyceps mycelia of gained, composition is similar to natural cordyceps height, and content is far away higher than other fermentation process.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
A preparation method for cordyceps mycelia, comprises the following steps:
(1) worm alive of Hepialidae larva is beaten into suspension;
(2) carry out sterilization treatment to step (1) gained suspension, sterilising temp is 121 DEG C, and the time is 30 minutes, and the suspension after sterilizing is stand-by as medium;
(3) step (2) gained medium is put into fermentation tank, inoculation Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind, wherein, the inoculum concentration of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is the 5wt% of medium, and the weight ratio of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is 1:2;
(4) control the fermentation condition of fermentation tank, fermentation tank throughput controls at 0.6m
3/ h, fermentation temperature 24 DEG C, fermentation time 106 hours, after fermentation, the centrifugal filtration of gained zymotic fluid obtains cordyceps mycelia, to cordyceps mycelia freeze drying, and obtains Chinese caterpillar fungus finished product mycelium powder after ultra-fine grinding.
The composition of Chinese caterpillar fungus finished product mycelium powder obtained by the present embodiment and the composition of commercially available cordyceps mycelia powder contrast as following table:
Can find out, the Chinese caterpillar fungus finished product mycelium powder main composition content that the present embodiment preparation method obtains is far above existing commercially available prod.
Embodiment 2
A preparation method for cordyceps mycelia, comprises the following steps:
(1) worm alive of Hepialidae larva is beaten into suspension;
(2) carry out sterilization treatment to step (1) gained suspension, sterilising temp is 121 DEG C, and the time is 30 minutes, and the suspension after sterilizing is stand-by as medium;
(3) step (2) gained medium is put into fermentation tank, inoculation Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind, wherein, the inoculum concentration of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is the 8wt% of medium, and the weight ratio of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is 2:1;
(4) control the fermentation condition of fermentation tank, fermentation tank throughput controls at 1.0m
3/ h, fermentation temperature 28 DEG C, fermentation time 112 hours, after fermentation, the centrifugal filtration of gained zymotic fluid obtains cordyceps mycelia, to cordyceps mycelia freeze drying, and obtains Chinese caterpillar fungus finished product mycelium powder after ultra-fine grinding.
The composition of Chinese caterpillar fungus finished product mycelium powder obtained by the present embodiment and the composition of commercially available cordyceps mycelia powder contrast as following table:
Can find out, the Chinese caterpillar fungus finished product mycelium powder main composition content that the present embodiment preparation method obtains is far above existing commercially available prod.
Embodiment 3
A preparation method for cordyceps mycelia, comprises the following steps:
(1) worm alive of Hepialidae larva is beaten into suspension;
(2) carry out sterilization treatment to step (1) gained suspension, sterilising temp is 121 DEG C, and the time is 30 minutes, and the suspension after sterilizing is stand-by as medium;
(3) step (2) gained medium is put into fermentation tank, inoculation Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind, wherein, the inoculum concentration of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is the 6wt% of medium, and the weight ratio of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is 1:1;
(4) control the fermentation condition of fermentation tank, fermentation tank throughput controls at 0.8m
3/ h, fermentation temperature 26 DEG C, fermentation time 110 hours, after fermentation, the centrifugal filtration of gained zymotic fluid obtains cordyceps mycelia, to cordyceps mycelia freeze drying, and obtains Chinese caterpillar fungus finished product mycelium powder after ultra-fine grinding.
The composition of Chinese caterpillar fungus finished product mycelium powder obtained by the present embodiment and the composition of commercially available cordyceps mycelia powder contrast as following table:
Can find out, the Chinese caterpillar fungus finished product mycelium powder main composition content that the present embodiment preparation method obtains is far above existing commercially available prod.
Above-mentioned is can understand and use invention for ease of those skilled in the art to the description of embodiment.Person skilled in the art obviously easily can make various amendment to these embodiments, and General Principle described herein is applied in other embodiments and need not through performing creative labour.Therefore, the invention is not restricted to above-described embodiment, those skilled in the art, according to announcement of the present invention, do not depart from improvement that scope makes and amendment all should within protection scope of the present invention.
Claims (7)
1. a preparation method for cordyceps mycelia, is characterized in that, comprises the following steps:
(1) worm alive of Hepialidae larva is beaten into suspension;
(2) sterilization treatment is carried out to step (1) gained suspension, stand-by as medium;
(3) step (2) gained medium is put into fermentation tank, inoculation Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind;
(4) control the fermentation condition of fermentation tank, carry out fermented and cultured, obtained cordyceps mycelia.
2. the preparation method of a kind of cordyceps mycelia according to claim 1, is characterized in that, in step (2), when carrying out sterilization treatment to suspension, temperature is 120-122 DEG C, and the time is 28-32 minute.
3. the preparation method of a kind of cordyceps mycelia according to claim 1, is characterized in that, in step (3), the inoculum concentration of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is 5 ~ 8wt% of medium.
4. the preparation method of a kind of cordyceps mycelia according to claim 1, is characterized in that, in step (3), the weight ratio of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is 1:2 ~ 2:1.
5. the preparation method of a kind of cordyceps mycelia according to claim 4, is characterized in that, in step (3), the weight ratio of Hirsutella sinensis bacterial classification and peacilomyce hepiahi bacterium kind is 1:1.
6. the preparation method of a kind of cordyceps mycelia according to claim 1, is characterized in that, in step (4), fermentation tank throughput controls at 0.6-1.0m
3/ h, fermentation temperature 24 ~ 28 DEG C, fermentation time 106-112 hour.
7. the preparation method of a kind of cordyceps mycelia according to claim 1, it is characterized in that, in step (4), after fermentation, the centrifugal filtration of gained zymotic fluid obtains cordyceps mycelia, to cordyceps mycelia freeze drying, and after ultra-fine grinding, obtain Chinese caterpillar fungus finished product mycelium powder.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101194721A (en) * | 2007-12-28 | 2008-06-11 | 王辉 | Ferment cordyceps sinensis bacterium mix powder and production method |
| CN101214265A (en) * | 2007-12-28 | 2008-07-09 | 王辉 | Chinese cephalosporin cordyceps sinensis mixed powder and preparation method |
| CN101214268A (en) * | 2007-12-28 | 2008-07-09 | 王辉 | Chinses hirsutella hepialid sinensis mixed powder and preparation method |
| CN101407767A (en) * | 2008-11-17 | 2009-04-15 | 贾景明 | Method for producing Chinese caterpillar fungus by fermentation |
| CN102696392A (en) * | 2012-05-30 | 2012-10-03 | 苏诚玉 | Method for increasing yield of natural cordyceps sinensis |
| CN102845218A (en) * | 2012-08-23 | 2013-01-02 | 大化金玉草生物科技有限责任公司 | Original ecological separation method of cordyceps sinensis hirsutella sinensis |
| CN103392513A (en) * | 2013-08-14 | 2013-11-20 | 西藏现代农业有限公司 | Ferment of aweto mycelia and application of ferment |
| CN103431368A (en) * | 2013-09-05 | 2013-12-11 | 东方中科生命科学有限责任公司 | Preparation method and comprehensive utilization of submerged fermentation product of cordyceps sinensis liquid |
| CN103432174A (en) * | 2013-08-27 | 2013-12-11 | 王辉 | Method for producing cordyceps sinensis powder by virtue of liquid fermentation on mixed bacterial strains |
-
2015
- 2015-06-18 CN CN201510341053.4A patent/CN104904496A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101194721A (en) * | 2007-12-28 | 2008-06-11 | 王辉 | Ferment cordyceps sinensis bacterium mix powder and production method |
| CN101214265A (en) * | 2007-12-28 | 2008-07-09 | 王辉 | Chinese cephalosporin cordyceps sinensis mixed powder and preparation method |
| CN101214268A (en) * | 2007-12-28 | 2008-07-09 | 王辉 | Chinses hirsutella hepialid sinensis mixed powder and preparation method |
| CN101407767A (en) * | 2008-11-17 | 2009-04-15 | 贾景明 | Method for producing Chinese caterpillar fungus by fermentation |
| CN102696392A (en) * | 2012-05-30 | 2012-10-03 | 苏诚玉 | Method for increasing yield of natural cordyceps sinensis |
| CN102845218A (en) * | 2012-08-23 | 2013-01-02 | 大化金玉草生物科技有限责任公司 | Original ecological separation method of cordyceps sinensis hirsutella sinensis |
| CN103392513A (en) * | 2013-08-14 | 2013-11-20 | 西藏现代农业有限公司 | Ferment of aweto mycelia and application of ferment |
| CN103432174A (en) * | 2013-08-27 | 2013-12-11 | 王辉 | Method for producing cordyceps sinensis powder by virtue of liquid fermentation on mixed bacterial strains |
| CN103431368A (en) * | 2013-09-05 | 2013-12-11 | 东方中科生命科学有限责任公司 | Preparation method and comprehensive utilization of submerged fermentation product of cordyceps sinensis liquid |
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Application publication date: 20150916 |