CN101263868A - Application of pickled vegetable in preparing medicaments and feed additive for preventing and controlling pig diarrhea - Google Patents
Application of pickled vegetable in preparing medicaments and feed additive for preventing and controlling pig diarrhea Download PDFInfo
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- CN101263868A CN101263868A CNA2008100528977A CN200810052897A CN101263868A CN 101263868 A CN101263868 A CN 101263868A CN A2008100528977 A CNA2008100528977 A CN A2008100528977A CN 200810052897 A CN200810052897 A CN 200810052897A CN 101263868 A CN101263868 A CN 101263868A
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Abstract
The invention relates to a pickle juice adopted during medicine preparation for preventing and controlling pig diarrhea and feed additive, which is characterized in that the pickle juice is obtained from various vegetable juice fermented by pickle lactic acid bacteria during the process of pickle maturing in pickle production; the pickle juice, concentrated liquid of pickle juice, and dried product or mixture of pickle juice are produced in the condition that the pickle is matured for 5 to 60 days under the temperature of 1 to 40 DEG C from preparation beginning or the pH value reaches to 3.0, and are applied to the medicine for preventing and controlling pig diarrhea and the feed additive; and the pickle lactic acid bacteria in the pickle juice is leuconostoc, lactobacillus and bifidobacterium. As a green and environment-protection natural active product, the pickle juice has the advantages of effectively curing the pig diarrhea, in particular to the secondary bacterial infection caused by viral disease of pigs, relieving symptom of the pig diarrhea, significantly improving immunity, mitigating stress response, and improving and maintaining gastrointestinal microflora balance; besides, the pickle juice also has the advantages of good efficacy, high safety, low cost, no drug resistance, no drug residue, and no poison and side effect.
Description
Technical field
The invention belongs to medicine and feed additive field, the application of particularly a kind of pickle juice in preparation control diarrhea of pigs medicine and feed addictive.
Background technology
Disease with diarrhea of pigs symptom is referred to as the diarrhea of pigs disease usually, and it can cause, and pig is malnutritive obstacle to occur with growing, and can cause death when serious.In large scale of pig farm was produced, because of all multifactor influences, the incidence of disease of grice diarrhoea and case fatality rate were all very high, had caused very big economic loss for the production of raising pigs.Grice diarrhoea is one of modal disease in the Swine Production, is the principal disease that causes piglet death.The pig at various ages all can be taken place clinically, and diarrhea of pigs is often caused by bacterial infection (as enteropathogenic E, salmonella, staphylococcus aureus etc.), virus infections (as rotavirus, parvovirus, coronavirus etc.), parasitic infection (as toxoplasm etc.).
The drug main of treatment pig alimentary infection disease will adopt antibiotic at present, but because above-mentioned various diseases cause of disease complexity, and the bacterial drug resistance bacterial strain constantly occur cause curative effect of medication imprecise, simultaneously, antibiotic can cause the imbalance of pig gastrointestinal bacterial flora, disturbs it to digest and assimilate function normally.Therefore be necessary to seek the new method of preventing and treating.
Pickles refer to the vegetables of pickling with salt (Chinese cabbage, radish, cucumber etc.) and go up flavouring such as adding chilli powder, garlic, green onion, and make it to produce through lactobacillus-fermented a kind of traditional food of acid.It is good to eat that it is vinegar-pepper, and be rich in nutritional labelings such as vitamin C, calcium, is the cuisines that people go with rice or bread.Pickles belong to lactobacillus-fermented food, pickled vegetable making generally all adopts high salinity, long-time pickled technology, its principle is: vegetables are in the high concentration salt solution of 5-10%, by means of natural beneficial microbe attached to vegetable surface (being lactic acid bacteria mainly), the juice of gushing out when utilizing vegetables infusion cutting process, carry out fermentation and acid slowly, reduce the pH value, utilize the hyperosmosis of salt simultaneously, suppress other harmful microbe growth jointly, through fermentation, obtain the pickles finished product.The sweat of pickles be unable to do without probio.As seen, the process of pickle fermentation is to suppress the process of the growth of harmful bacteria by beneficial bacteria (lactic acid bacteria).
Pickle lactic acid bacteria has and prevents antibiotic and bactericidal effect, strengthens the effect of immunity of organisms.Pickle lactic acid bacteria can suppress and the kill harmful bacterium, and prevents the expansion of these bacteriums.Curative effect for the checking pickle lactic acid bacteria, discover for one of professor Jiang Sixu of Seoul, South Korea university hosting several years ago, research group utilizes the pickle lactic acid bacteria nutrient solution, feed the pig that suffers from breathing problems such as " H9N2 " type swine flu, bronchus influenza, after one week, the sick pig of the overwhelming majority is fully recovered, and is evident in efficacy; And the pig of not taking lactic acid bacteria culture solution has over half dying.Experiment finds that the content of lactic acid bacteria has only about 10,000 in the pickles that every gram had just been pickled, but through after the cold fermentation, can increase to 6,300 ten thousand.Also contain more than 3000 kind of microorganism in the pickles, the some of them microorganism can be controlled the harmful bacteria that causes people's gastritis, gastric ulcer.They in addition think that during the SARSs in 2003, the main cause of one routine SARS does not take place in Korea S, exactly because the edible pickles of Korean.
Pickle juice is the product that obtains during the fermentation by the pickles as traditional food.Above-mentioned pickle juice by human intake, stability height, is the natural probiotics that a kind of antibacterial ability is strong, antimicrobial spectrum is extensive by absorbing for a long time in the diet easily.Pickle lactic acid bacteria function attraction in the control intestines and stomach is more and more paid close attention to, and people get more and more to having beneficial research and application liquid of giving birth to the lactic acid bacteria of function.Pickle juice contains various lactobacillus and natural antibacterial active materials such as pickle lactic acid bacteria, lactein, lactic acid, hydrogen peroxide and acetic acid.Lactic acid bacteria in the pickles changes into lactic acid to other with the sugar part in the vegetables that salt down, plays making the pickles delicious flavour and killing pathogenicity.Pickles are because process by fermentation contains very many lactic acid bacterias, and average 1 gram pickles just contain several hundred million lactic acid bacterias.Body picked-up lactic acid bacteria can not only increase the quantity of beneficial bacterium, suppresses harmful bacterium, prevents abnormal fermentation, improves the intestinal flora environment, can also make intestinal flora maintain the state of relative equilibrium.The fermentation pickles produce a certain amount of organic acid, alcohol, Ester etc., not only give the special local flavor of pickles, prevent putrid and deteriorated, can also improve a poor appetite, promote digestion.Pickles are cuisines that people go with rice or bread, and pickle juice and pickles do not appear in the newspapers in the application and the research of diarrhea of pigs.
Summary of the invention
The present invention seeks to overcome deficiency of the prior art, the application of a kind of pickle juice in preparation control diarrhea of pigs medicine and feed addictive is provided.
Implementation of the present invention is as follows for achieving the above object: the application of a kind of pickle juice in preparation control diarrhea of pigs medicine and feed addictive, it is characterized in that described pickle juice obtains from the pickles maturing process, in the process of pickled vegetable making, fermentation forms various vegetable juice through pickle lactic acid bacteria; Described pickles all reach 3.0 1-40 ℃ of following slaking 5-60 days or pH value from beginning preparation, make pickle juice, pickle juice concentrate, pickle juice drying thing or mixture and are applied to prevent and treat in diarrhea of pigs medicine and the feed addictive.
Pickle lactic acid bacteria belongs to the Leuconostoc lactic acid bacteria in the described pickle juice, milk-globule belongs to lactic acid bacteria, lactobacillus (comprising the bar-shaped subspecies of lactobacillus, Lactobacillus brevis, Bu Shi lactobacillus, Lactobacillus casei cheese subspecies, the bar-shaped Lactobacillus plantarum of Lactobacillus delbrueckii etc.), Bifidobacterium lactic acid bacteria.
The invention has the beneficial effects as follows: after pickle lactic acid bacteria enters body, will in enteron aisle, breed, produce lactic acid, acetate and some antibacterial materials, the pH value of enteron aisle is reduced, thereby can suppress pathogenic bacteria and the growth and breeding that is harmful to the health bacterium, can play the effect of antibiotic diseases prevention.Simultaneously, the balance that gut flora has also been kept in the raised growth of lactic acid bacteria breeding has played whole intestines effect.The generation of lactic acid bacteria and metabolite energy inducing interferon and mitogen, the activation NK also produces immune globulin antibody, thus the function of activated macrophage, the immunocompetence of enhancing body.
The present invention has certain bidirectional modulation body's immunological function, for the control of pig virus disease provides new way.This products material all derives from crude vegetable, reaches the viricidal effect that presses down by the antipathogen mechanism (as immune system, complement system etc.) of transferring the body inherence.Medicine green natural, noresidue endanger, and meet the theory of green health.Medicine of the present invention is primarily aimed at the prevention of bird diarrhea disease and treatment has very significant advantage.Clearing heat and detoxicating ability is strong, clinically is mainly used in treatment, thereby alleviates the symptom of disease, increases disease fowl appetite, promotes the rehabilitation of disease fowl.
The raw material sources that adopted are extensive, and preparation technology is simple, can prevent and treat disease and syndrome thereof that bird often sends out safely and effectively.This medicine is drug residue free in use, has no side effect, and is little to the body excitant, is difficult for producing drug resistance, solved the puzzlement of outlet restriction fully.This cured substance rate height, production cost is low, applied range.
Experiment showed, after deliberation to the present invention is based on pickle lactic acid bacterias a large amount of in pickle lactic acid bacteria above-mentioned functions and the pickle juice that attempt Journal of Sex Research and find, pickle juice has the function of prevention and treatment diarrhea of pigs.Pickle juice of the present invention is effectively treated diarrhea of pigs in the application of preparation control diarrhea of pigs medicine, and especially the pig virus disease causes the symptomatic treatment of secondary bacterial infection, has alleviated the symptom of diarrhea of pigs.Pickle juice of the present invention can obviously improve immunity, slows down stress reaction, improves and safeguards the gastrointestinal bacterial flora balance.Have good effect, safe, have no drug resistance, with low cost, drug residue free, Nonpoisonous, non-environmental-pollution are the natural active matters of environmental protection.
The specific embodiment
Below in conjunction with embodiment, to details are as follows according to the specific embodiment provided by the invention:
Pickle juice described in the present invention obtains from the pickles maturing process, and in the process of pickled vegetable making, fermentation forms various vegetable juice through pickle lactic acid bacteria; Described pickles all reach 3.0 1-40 ℃ of following slaking 5-60 days or pH value from beginning preparation, make pickle juice, pickle juice concentrate, pickle juice drying thing or mixture and are applied to prevent and treat in diarrhea of pigs medicine and the feed addictive.
The application of described pickle juice in preparation control diarrhea of pigs medicine and feed addictive is characterized in that pickle lactic acid bacteria is that Leuconostoc lactic acid bacteria, milk-globule belong to lactic acid bacteria, lactobacillus, Bifidobacterium lactic acid bacteria in the described pickle juice.
Lactic acid bacteria can suppress the growth of pathogenic bacteria in the intestines and stomach and suppress it to be attached to intestines and stomach in the pickle juice, and pickle juice can be used as medicine or feed addictive, is used to prevent and treat diarrhea of pigs.
Embodiment 1
The preparation process of pickles (Chinese cabbage) juice
1, preparation condiment: spring onion are cut sth. askew into thread, onion is cut into thread, and the peeling ginger is smashed into mud to pieces, and leek is cut into 1-2 centimetre of segment, and ternip is wiped into filament.Get spring onion 0.7g, onion 0.5g, ginger 0.8g, leek 0.8g, ternip 1.0, salt 5g, Chinese prickly ash 0.1g, paprika 0.1g, water 91.0g boiled behind the mixing 10-15 minute, was cooled to room temperature.
2, pickled vegetable making: choosing is bright in colour, anosis worm harm, and light green fresh Chinese cabbage is used the clear water flushing, and it is standby that the Chinese cabbage that washes down is placed upside down in online water 1-4 hour controlled naturally of cold dish.Segment is cut in taking-up after removing root, gets 500g Chinese cabbage then and puts into pickle jar, adds the above-mentioned preparation condiment of 1000g again.Chinese cabbage is dipped in the ingredients solution fully, seal tight, the fermentation slaking promptly got pickles in 10-30 days.
3, the pickles that perform are taken out from altar, after directly putting into food masher and pulverizing, make homogenate, put into branch packaging container or polybag, add the pickle juice of 0.5-1 times of volume then.Deposit in after the packing in 3-15 ℃ the environment and preserve.
Pig is fed after pickle juice and the pickles, can take in metabolism products such as a large amount of lactic acid bacterias and lactic acid, and they can regulate the intestinal microecology balance, reach the purpose of prevent disease and health care.Commodity such as domestic and international some kinds of medicines that contain lactic acid bacteria and oral liquid carry out the viable count testing result and show that the live lactobacillus number is higher than these type of commodity far away in the pickle juice, and good and cheap, instant.Pickle juice and pickles can become and have cheap, natural microecologic regulator efficiently.In addition, utilize pickle juice to produce natural microecologic regulators such as high-quality lactic acid bacteria health food or medicine and be used for prevention and treatment diarrhea of pigs, this has important practical value to the general level of the health that improves Swine, the incidence of disease that reduces disease.
Embodiment 2
The preparation process of pickles (wild cabbage) pickle juice
1, raw material is handled: wild cabbage is removed crust clean, the drop branch that anhydrates is cut into 6 parts.
2, weigh batching: take by weighing materials by following prescription, standby.Prescription: 10 kilograms in water, 5 kilograms of wild cabbages, salt 380 grams, anistree 15 grams, sugar 600 grams, Chinese prickly ash 7.5 grams, pepper 12 grams, dried orange peel 7.5 grams, 2 in capsicum, garlic 4 lobes, citric acid (or vinegar) 0.5 gram.
3, the preparation of pickles water: by above-mentioned prescription water, salt, sugar, citric acid (or vinegar) mixing are made pickles water, place stand-by.
4, dress altar fermentation: the pickle jar outwash is dried, below the wild cabbage fragment is placed on, pour pickles water after installing into,, add liquor in a small amount at last, cover the altar lid and seal with clear water until the submergence raw material.Be that maturation promptly gets pickles after 20-40 days.
5, pickles are squeezed the juice, vacuum promptly gets pickle juice after concentrating, and pickle juice is put into branch packaging container or polybag, deposits in the low temperature environment.
The lactic acid bacteria bacteria containing amount can reach 10 in the pickle juice
8-10
9Individual/ml, the pH value stabilization is 3.0.Common lactic acid bacteria mainly contains Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus brevis, Leuconostoc mesenteroides and Bifidobacterium etc. in pickles.The manufacturing process of pickles is under high salt anoxic environment, and the growth of lactic acid bacteria is preponderated, and produces a large amount of lactic acid, has suppressed the growth of other bacterium.Lactic acid not only makes finished product have tart flavour, simultaneously can with the alcohols material generation esterification that produces in the sweat, produce ethyl lactate etc., make finished product have fragrance; Simultaneously, partially protein decomposes generation amino acid in the sweat, has very high nutritive value.
Embodiment 3
The method that the throw type leaven fermentation pickled vegetable is produced pickle juice is as follows:
Screening and efficient fermentation technique research with the throw type leaven bacterial classification, the pickle fermentation special lactobacillus strain of highly effective and safe is cultivated in i.e. screening, as Lactobacillus plantarum B4, lactobacillus curvatus A8,, it is prepared into directly is used for the leavening of fermentation pickled vegetable again through the High Density Cultivation enrichment.This leavening dry powder viable count can reach 1 * 10
11About cfu/g, drop into the 3Kg leavening in 1 ton of vegetables.The surface moisture that dries in the air after vegetables are cleaned up is put into the saline solution that pickle jar adds 6-8%, makes saline solution flood vegetables fully, covers the altar lid, pickles at 25 ℃ of bottom fermentations can ripely be edible pickles in 1-5 days.Pickles are squeezed the juice, and vacuum promptly gets pickle juice after concentrating, and pickle juice is put into branch packaging container or polybag, deposits in the low temperature environment.
The throw type leaven fermentation pickled vegetable has the following advantages: can keep stable quality, enlarge links such as cultivation in the middle of avoiding; Simplify production technology, shortened the production cycle, can make fermentation time foreshorten to 1/2～1/3 of spontaneous fermentation, reduced cost; Number of viable in the pickle juice exceeds more than 100 times than spontaneous fermentation.
Application example
In October, 2007, pig farm, Ji County, Tianjin 10-45 age in days grice diarrhoea, (viable lactic acid bacteria quantity is 1 * 10 with adopting specific embodiment 1 to make pickle juice in disease time is no more than 24h
9Individual/g) treatment and observing effect.45 of 10-28 age in days piglets are divided into three groups of A, B, C, and 15 every group have been carried out the diarrhea treatment contrast test.The A group is pressed the 10ml/ head, and twice, 3 day on the one is a course of treatment.The B group is pressed the oral Ciprofloxacin of 5mg/Kg, and every day 2 times, 3 days is a course of treatment.The C group takes drugless medication, as negative control.A group the efficient of piglet is 79.8% as a result; It is 84.8% that B organizes piglet efficient; It is 25.5% that C organizes pig efficient.The A group is efficient extremely remarkable with C group difference.The effect highly significant that shows pickle juice treatment diarrhea of pigs.
The using method of pharmaceutical composition of control grice diarrhoea is: will contain a kind of pharmaceutical composition of preventing and treating grice diarrhoea and be by weight percentage 0.5% ratio and evenly admix in the feed, and allow the piglet free choice feeding observe prevention effect.
Animal experiment: the piglet of 90 10-20 ages in days is divided into two groups of A, B, C, and 30 every group have been carried out 15 days prevention diarrhea of pigs contrast tests.The A group is admixed a kind of green premixing material of preventing and treating grice diarrhoea of embodiment 1 preparation in feed by weight 1.0% ratio, the B group is admixed Ciprofloxacin Lactate by weight 0.5% ratio in feed, do not contain the premix of the pharmaceutical composition of preventing and treating grice diarrhoea in the C group feed.Result of the test is that the protective rate of A group reaches 100.0%, and the B group is 100.0%, and the C group only is 82%, and A group protective rate and C group difference are extremely remarkable.The effect that shows pickle juice prevention diarrhea of pigs is remarkable.
The experimental study of pickle juice control grice diarrhoea:
The preparation of pickle juice: adopt embodiment 2 to make pickle juice, viable lactic acid bacteria quantity is 1 * 10
9Cfu/g.Enrofloxacin: adopt Haikang, Guangdong animal pharmaceutical factory product.
The feeding and management of test pig: all are supported or the cement flooring is flat supports in the childbirth drylot feeding of the same terms with its sow for the examination piglet, and routine disinfection is 1 time weekly.
According to clinical observation, totally 32 of the piglets with obvious symptom of diarrhea of selection pig farm, suburbs, Tianjin 10～28 ages in days.
The piglet that is diagnosed as the availability test of diarrhoea is divided into two groups:
Test I group: 14 of 10～28 ages in days; Press 10ml/ the oral pickle juice of body weight, every day 2 times, 3 days is a course of treatment.
Test II group: 18 of 10～28 ages in days; Press the oral Enrofloxacin of RD, every day 2 times, 3 days is a course of treatment.
Curative effect judging standard:
During the treatment, examine the elasticity of the state of mind, appetite, stool situation, circumanal humidity and the anal sphincter of respectively organizing the disease pig every day sooner or later for twice.After the drug withdrawal 3 days every group choose 1 immediately and cut open and kill, observe the enteron aisle pathology, it is fixing to get intestinal tissue (jejunal segment), histotomy to be done.
Cure: treatment 24～48h ight soil is strip, and color, smell are normal, and piglet spirit, sucking or material feeding are normal, the symptom complete obiteration.
Take a turn for the better: treatment 48～72h ight soil proterties takes a turn for the better, moisture obviously reduces clinical symptom remission or basic the disappearance.
Invalid: behind the treatment 72h, ight soil number of times and proterties do not have improvement, even the state of an illness increases the weight of.
Cure the number revolution of becoming reconciled and add up to total significant figure; Invalid number comprises death toll.
As seen from Table 1, pickle juice treatment 10-28 age in days grice diarrhoea total effective rate reaches 92.9%, and is basic identical with Enrofloxacin treatment group; The 48h overwhelming majority piglet state of an illness is controlled after medication, but with regard to the whole course of disease, the curative effect of pickle juice is all significantly better than other several anti-infectives.Experimental study shows that pickle juice treatment grice diarrhoea effect is remarkable.Pickle juice is better as prevention or medicine effect, can not produce any drug resistance, but prolonged application, the resistance to the action of a drug of avoiding adopting drug therapy such as Enrofloxacin to occur.
Table 1 10～28 ages in days diarrhoea piglet treatment results relatively
The oral pickle juice treatment of piglet diarrhoea, basic identical with Enrofloxacin; Better to the piglet result of treatment of falling ill early stage.Cause the reason of grice diarrhoea a lot, when particularly being secondary to some deadly infectious disease, still must cooperate corresponding countermeasures just can achieve good results.
Pickle juice of the present invention is made powder-injection or liquid drugs injection at pharmaceutically acceptable carrier, and it can be used as injection also can make oral formulations.
Pickle juice of the present invention removes the diarrhoea that viral diseases such as directly applying to the infection of treatment porcine rotavirus causes, is applicable to the treatment in the bacterial infection disease simultaneously.Pickle juice wide material sources of the present invention, and the preparation method is easy, easily applies aborning.
Above-mentioned with reference to the embodiment detailed description that the application in preparation control diarrhea of pigs medicine and feed addictive is carried out to this pickle juice; be illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (4)
1, the application of a kind of pickle juice in preparation control diarrhea of pigs medicine and feed addictive is characterized in that described pickle juice obtains from the pickles maturing process, in the process of pickled vegetable making, fermentation forms various vegetable juice through pickle lactic acid bacteria; Described pickles all reach 3.0 1-40 ℃ of following slaking 5-60 days or pH value from beginning preparation, make pickle juice, pickle juice concentrate, pickle juice drying thing or mixture and are applied to prevent and treat in diarrhea of pigs medicine and the feed addictive.
2, the application of pickle juice according to claim 1 in preparation control diarrhea of pigs medicine and feed addictive is characterized in that pickle lactic acid bacteria is that Leuconostoc lactic acid bacteria, milk-globule belong to one of lactic acid bacteria, lactobacillus, Bifidobacterium lactic acid bacteria in the described pickle juice.
3, the application of pickle juice according to claim 1 in preparation control diarrhea of pigs medicine and feed addictive is characterized in that the medicinal application of described pickle juice as the control diarrhea of pigs.
4, the application of pickle juice according to claim 1 in preparation control diarrhea of pigs medicine and feed addictive is characterized in that the additive application of described pickle juice as control diarrhea of pigs feed.
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| CNA2008100528977A CN101263868A (en) | 2008-04-25 | 2008-04-25 | Application of pickled vegetable in preparing medicaments and feed additive for preventing and controlling pig diarrhea |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404698A (en) * | 2013-08-08 | 2013-11-27 | 辽宁禾丰牧业股份有限公司 | Soft pellet feed and preparation thereof |
| CN104286414A (en) * | 2014-09-24 | 2015-01-21 | 邵素英 | Nutritional feed additive and preparation method thereof |
| CN104286465A (en) * | 2014-09-24 | 2015-01-21 | 邵素英 | Feed additive product for dairy cattle and production method thereof |
-
2008
- 2008-04-25 CN CNA2008100528977A patent/CN101263868A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404698A (en) * | 2013-08-08 | 2013-11-27 | 辽宁禾丰牧业股份有限公司 | Soft pellet feed and preparation thereof |
| CN103404698B (en) * | 2013-08-08 | 2015-01-28 | 辽宁禾丰牧业股份有限公司 | Soft pellet feed and preparation thereof |
| CN104286414A (en) * | 2014-09-24 | 2015-01-21 | 邵素英 | Nutritional feed additive and preparation method thereof |
| CN104286465A (en) * | 2014-09-24 | 2015-01-21 | 邵素英 | Feed additive product for dairy cattle and production method thereof |
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Application publication date: 20080917 |