Disclosure of Invention
The invention aims to provide a liquid-based thin-layer cell slide making clamp which can reduce the outflow of cells on a glass slide along with water, thereby ensuring the collection of the cells on the glass slide.
The technical purpose of the invention is realized by the following technical scheme: a liquid-based thin-layer cell slide making clamp comprises a sedimentation pipe and a fixing seat, wherein the sedimentation pipe is vertically arranged at one end of the upper part of the fixing seat, the bottom of the sedimentation pipe is communicated with the fixing seat, slots are respectively arranged on two side walls of the fixing seat, a slide glass is connected in the slots in a sliding manner, a baffle is arranged between the inner walls of the lower end of the sedimentation pipe, the lower end of the baffle is abutted against the slide glass, the baffle is arranged close to the central position of a slide making machine, the length of the baffle is smaller than the diameter of the sedimentation pipe, the space formed by the sedimentation pipe and the slide glass is divided into a cell settling area and a water absorption area by the baffle, the cell settling area is arranged close to the central position of the slide making machine, two ends of the baffle are respectively and fixedly connected to the inner wall of the sedimentation pipe, a movable plate which is arranged on, and an extension spring is arranged above the cell precipitation zone on the movable plate, one end of the extension spring is fixed on the movable plate, the other end of the extension spring is fixed on the inner wall of the sedimentation tube, and the sedimentation tube is provided with water-absorbing cotton on the inner wall of the water-absorbing zone.
By adopting the technical scheme, the sedimentation tube is vertically arranged at one end of the upper part of the fixed seat, the bottom of the sedimentation tube is communicated with the fixed seat, a closed space is formed by the sedimentation tube and a glass slide which is connected with the fixed seat in a sliding way, the baffle plate and the moving plate are arranged on the inner wall of the sedimentation tube, the space formed by the baffle plate and the moving plate is divided into a cell precipitation area and a water absorption area, liquid-based cells are limited in the cell precipitation area when entering the sedimentation tube, the water absorption area does not contain the liquid-based cells, when the sheet making clamp is placed on the sheet making machine for centrifugal motion, the cell precipitation area is arranged close to the central position of the sheet making machine, therefore, when the sheet making machine starts to work, the moving plate can rotate towards the water absorption area due to the action of centrifugal force as one end of the moving plate is hinged with the inner wall of the, at the moment, the extension spring is stretched to have elasticity, the baffle is positioned above the diameter of the sedimentation tube, and the movable plate is arranged at the upper end of the baffle, so that the movable plate opens the cell sedimentation zone when rotating, and then the cell sedimentation zone is communicated with the water absorption zone, water in the liquid-based cells enters the water absorption zone due to centrifugal force, the cells are adsorbed on the glass slide in the cell sedimentation zone, the baffle prevents the cells on the glass slide from entering the water absorption zone, the water absorption cotton is arranged on the inner wall of the sedimentation tube in the water absorption zone and is used for absorbing the water entering the water absorption zone, the water in the liquid-based cells is absorbed by the water absorption cotton, meanwhile, the arrangement of the baffle prevents the cells from being absorbed by the water absorption cotton, so that the water in the liquid-based cells is separated from the cells, the loss of the cells along with the water flow flowing out of the sedimentation tube is reduced, the collection of the cells on the glass slide is ensured, when the slide preparation machine stops moving, the elastic force of the extension, thereby shutting off the cell pellet zone.
The invention is further configured to: the sedimentation pipe endotheca is equipped with the filter cup, the filter cup rim of a cup is equipped with the backup pad of outside extension, the backup pad restriction filters the cup and moves down along the sedimentation pipe, the through-hole has been seted up in the position that is located directly over the cell precipitation district at the filter cup bottom of a cup, be equipped with the filtration membrane that seals the through-hole on the through-hole pore wall.
Through adopting above-mentioned technical scheme, the backup pad makes the filter cup fixed in the sedimentation tube, and the setting of filter cup has accelerated the speed that the liquid-based cell flows into in the sedimentation tube, and the through-hole has been seted up on the filter cup bottom of cup on the cell precipitation district, and the setting of through-hole is convenient for the liquid-based cell flow in the cell precipitation district, and the filtration membrane setting on the through-hole has filtered the impurity in the liquid-based cell, avoids impurity and cell to adhere to on the slide glass together, and then influences the collection of cell.
The invention is further configured to: the filter is characterized in that a limiting block is arranged on the outer wall of the filter cup, and a limiting groove for the limiting block to slide into is formed in the inner wall of the sedimentation pipe.
Through adopting above-mentioned technical scheme, when filter the cup and put into the sedimentation pipe, the stopper on the filter cup outer wall slips into the spacing inslot of sedimentation pipe, and stopper and spacing inslot's cooperation is used and has been restricted the rotation of filter cup in the sedimentation pipe, avoids the through-hole to be changeed to the district top that absorbs water, is absorbed by the cotton that absorbs water in the district that absorbs water, influences the collection of cell.
The invention is further configured to: the wall of the filter cup above the water absorption area is provided with a guide surface extending from the cup mouth of the filter cup to the filter membrane.
Through adopting above-mentioned technical scheme, the setting up of spigot surface makes liquid-based cell when getting into the filter bowl, flows fast and filters on the filtration membrane, has improved the speed that the cell was gathered, and avoids liquid-based cell to be detained on filtering the cup wall.
The invention is further configured to: the outer wall of the filter cup gradually extends towards the center of the filter cup from the cup mouth to the cup bottom.
Through adopting above-mentioned technical scheme, will filter the cup outer wall and extend to filtering the cup center from the rim of a cup to the bottom of a cup direction gradually for filter cup lower extreme diameter is less than the upper end diameter, is convenient for put into the sedimentation pipe fast with filtering the cup.
The invention is further configured to: the baffle is obliquely arranged towards the direction far away from the extension spring.
Through adopting above-mentioned technical scheme, baffle slope sets up and is convenient for when the film clamp is in centrifugal state, and the water in the cell sedimentation district can flow to the district that absorbs water along the baffle of slope, is convenient for accelerate the outflow of the water in the cell sedimentation district, reduces the centrifugation time.
The invention is further configured to: and a placing groove matched with the outer wall of the sedimentation tube is arranged on one side of the glass slide facing the sedimentation tube.
Through adopting above-mentioned technical scheme, setting up of standing groove makes the cell adsorbed in the standing groove, when avoiding slide to take out from the fixing base, the operator mistake is met slide top, influences the purity that cell was gathered on the slide.
The invention is further configured to: the lower end of the settling tube is provided with a sealing rubber ring, and the lower end of the settling tube is provided with an annular groove for embedding the sealing rubber ring.
Through adopting above-mentioned technical scheme, the setting of annular is convenient for the fixed of sealing rubber circle and sedimentation tube, and the sealing rubber circle sets up when the film clamp is in centrifugal state at the lower extreme of sedimentation tube, and restriction cell and water flow channel subside outside of the tube, and then influence the use of whole film clamp.
The invention is further configured to: and the groove wall of the annular groove is provided with an inclined plane extending from the lower end of the settling tube to the direction far away from the axis of the settling tube.
Through adopting above-mentioned technical scheme, the setting on inclined plane has increased the area of contact of annular and sealing rubber circle, has increased the frictional force between annular and the sealing rubber circle for better fixing in the annular of sealing rubber circle.
The invention is further configured to: the slide glass fixing device is characterized in that a pressing plate is arranged at the lower end of the slide glass, sliding grooves parallel to the inserting grooves are formed in two side walls of the fixing base respectively, the pressing plate is connected with the sliding grooves in a sliding mode, and pressing blocks for pressing the slide glass upwards in an extruding mode are arranged on the pressing plate.
Through adopting above-mentioned technical scheme, spout and slot parallel arrangement are convenient for the clamp plate and are slided into in the fixing base, and when the clamp plate slided into the fixing base, the compact heap on the clamp plate has compressed tightly the slide glass for slide glass closely laminates with the lower extreme of sedimentation tube, guarantees that the film-making presss from both sides when centrifugal state, and the liquid-based cell on the slide glass is in the sedimentation tube all the time.
In conclusion, the invention has the following beneficial effects: the arrangement of the baffle plate in the settling tube limits the cells adsorbed on the glass slide to flow to the water absorption area, the cells are prevented from being absorbed by the water absorption cotton in the water absorption area, the arrangement of the moving plate is convenient for the water in the liquid-based cells to flow out of the cell settling area, the arrangement of the extension spring on the moving plate is convenient for fixing the moving plate, and the moving plate is prevented from being pushed open by the liquid-based cells.
Example 1: a liquid-based thin-layer cell sheet making clamp is shown in figures 1 and 3 and comprises a fixed seat 1 and a settling tube 11 vertically arranged on the fixed seat 1, wherein the bottom of the settling tube 11 is communicated with the fixed seat 1, a ring groove 117 is arranged at the lower end of the settling tube 11, an inclined surface 1171 extending from the lower end of the settling tube 11 to the direction far away from the axis of the settling tube 11 is arranged on the wall of the ring groove 117, and a sealing rubber ring 116 is fixed in the ring groove 117 so as to limit cells and water to flow out of the settling tube 11 when the sheet making clamp is in a centrifugal state; two side walls of the fixed seat 1 are respectively provided with a slot 112 (as shown in fig. 2), a glass slide 111 is connected in the slot 112 in a sliding manner, one surface of the glass slide 111 facing the sedimentation tube 11 is provided with a placing groove 1111 (as shown in fig. 4) for embedding the sedimentation tube 11, so that when the glass slide 111 is taken out from the fixed seat 1, an operator is prevented from mistakenly touching the upper part of the glass slide 111 to influence the purity of cell collection on the glass slide 111; when the glass slide 111 slides to the slot 112, a closed space is formed with the bottom of the sedimentation tube 11, at this time, a baffle 113 abutting against the upper end of the glass slide 111 is fixedly connected between the inner walls of the lower end of the sedimentation tube 11, the baffle 113 divides the space formed by the sedimentation tube 11 and the glass slide 111 into a cell precipitation zone 114 and a water absorption zone 115, the cell precipitation zone 114 is arranged near the center of the pelleter, a moving plate 1131 which is in the same plane with the baffle 113 is arranged at the upper end of the baffle 113, one end of the moving plate 1131 is hinged with the inner wall of the sedimentation tube 11, the other end of the moving plate 1131 abuts against the inner wall of the sedimentation tube 11, so that the moving plate 1131 can rotate around the hinged part in the sedimentation tube 11, in order to avoid the liquid-based cells pushing the moving plate 1131, a tension spring 1132 is arranged above the cell precipitation zone 114 of the moving plate 1131, the moving plate 1131 is fixed by the extension spring 1132, so that the moving plate 1131 is not squeezed by liquid-based cells and rotates, the baffle 113 is obliquely arranged in the direction away from the extension spring 1132, so that water in the cell settling zone 114 can flow to the water absorption zone 115 along the oblique baffle 113, the outflow of the water in the cell settling zone 114 is facilitated, the centrifugation time is reduced, the baffle 113 is arranged at the position close to the center of the pelleter, and the length of the baffle 113 is smaller than the diameter of the sedimentation tube 11, so that the moving plate 1131 cannot be clamped by the inner wall of the sedimentation tube 11 when moving; and water absorption cotton 1151 is arranged on the inner wall of the settling pipe 11 in the water absorption area 115.
The specific operation process comprises the following steps: firstly, slide glass 111 in the slot 112, let in liquid-based cell in the cell sedimentation zone 114 in the sedimentation tube 11 and precipitate, make the cell adsorbed on slide glass 111, later put the slide making clamp into the slide making machine, carry out centrifugal treatment, movable plate 1131 rotates around the position articulated with sedimentation tube 11 inner wall because of the effect of centrifugal force this moment, and then open cell sedimentation zone 114, make the water in the cell sedimentation zone 114 flow to water absorption zone 115 because of centrifugal effect, extension spring 1132 is in the tensile state and has elasticity this moment, the moisture that gets into water absorption zone 115 is absorbed by water absorption cotton 1151, and then need not pour out the moisture in the sedimentation tube 11 after the centrifugation finishes, and extension spring 1132 resets movable plate 1131.