CN220583894U

CN220583894U - Disposable fecal sample processor

Info

Publication number: CN220583894U
Application number: CN202321626004.1U
Authority: CN
Inventors: 顾嘉迪
Original assignee: China Science And Technology Investment Zhejiang Biomedical Co ltd
Current assignee: China Science And Technology Investment Zhejiang Biomedical Co ltd
Priority date: 2023-06-26
Filing date: 2023-06-26
Publication date: 2024-03-12
Anticipated expiration: 2033-06-26

Abstract

The utility model provides a disposable fecal sample processor, which comprises a sealing cover, a sample container, a sealing guide seat and a first filter seat, wherein the top of the sample container is provided with a sample inlet, the sealing cover is connected to the sample inlet of the sample container, the bottom of the sample container is provided with a sample outlet, the sample outlet is in a frustum shape gradually widened from inside to outside of the sample container, the sealing guide seat is sleeved outside the sample container, the bottom of the sealing guide seat is provided with a sealing plug, the sealing guide seat is circumferentially provided with a plurality of sample passing openings at the periphery of the sealing plug, the inner wall of the sealing guide seat is provided with a buckle, the outer wall of the sample container is provided with a first clamping groove and a second clamping groove corresponding to the buckle, the first filter seat is connected to the bottom end of the sealing guide seat and is communicated up and down, and the inside of the first filter seat is provided with a first filter layer. According to the disposable fecal sample processor, through the integrated flow design, the operation steps are simplified, and the working efficiency is improved.

Description

Disposable fecal sample processor

Technical Field

The utility model belongs to the technical field of experimental sample processors, and particularly relates to a disposable fecal sample processor.

Background

The normal feces mainly consist of food residues which are not absorbed after digestion, digestive tract secretions, a large amount of bacteria, inorganic salts, water and the like. The main purpose of fecal examination is: 1. knowing whether the digestive tract has inflammation, hemorrhage, parasitic infection, malignant tumor and the like; 2. indirectly judging the functional status of gastrointestinal, pancreatic, hepatobiliary systems according to the characters, the compositions and the like of the excrement; 3. knowing whether the distribution of the intestinal landform flora is reasonable or not, checking whether pathogenic bacteria exist in the feces or not, and assisting in diagnosing intestinal infectious diseases.

After sampling, the fecal sample is subjected to a certain solution treatment and then subjected to the required experiment to detect the specific substance content, for example: hemoglobin, tumor-associated antigens, specific DNA and RNA, play a role in diagnosing or aiding in the diagnosis of disease clinically. Other solid or semi-solid samples, such as soil, food, tablets, etc., also require specific solutions to be dissolved, filtered, separated, etc., prior to performing a specific assay, to achieve the basic preconditions of detection.

In the prior art, the sample processing process is performed in multiple steps in a laboratory, including weighing the sample and the dissolution solution, transferring to another device for filtration, separation, collection and sample addition. Complicated operation steps, easy individual difference generation and influence on detection results. Meanwhile, excessive operation steps are easy to generate sample splashing and pollute the components.

Disclosure of Invention

In view of the above technical problems, the utility model provides a disposable fecal sample processor, which aims to simplify operation steps and improve working efficiency through the design of an integrated sampling and filtering flow of fecal samples.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a disposable fecal sample treater, includes sealed lid, sample container, sealed guide seat, first filtration seat, sample container's top is equipped with the application of sample mouth, sealed lid is connected sample mouth department of sample container, sample container's bottom is equipped with the play appearance mouth, the shape of appearance mouth is by the interior frustum shape that gradually widens outward of sample container, sealed guide seat is open-ended column, sealed guide seat suit is in sample container is outer, sealed guide seat's bottom be equipped with the sealing plug that appearance mouth shape suited, sealed guide seat is in the periphery of sealing plug is equipped with a plurality of appearance mouthfuls in circumference, sealed guide seat inner wall is equipped with the buckle be equipped with on sample container's the outer wall with the corresponding first draw-in groove of buckle and second draw-in groove, first filtration seat is connected the bottom of sealed guide seat, first filtration seat link up from top to bottom, the inside of first filtration seat is equipped with first filter layer.

Further, the disposable fecal sample processor further comprises a second filter seat, wherein the second filter seat is connected with the bottom end of the first filter seat, the second filter seat is vertically communicated, and a second filter layer for adsorbing specific DNA, RNA and antigens is arranged in the second filter seat.

Further, a disposable fecal sample processor still include out the liquid seat, go out the liquid seat and connect the bottom of first filter seat, go out the liquid seat and link up from top to bottom, go out the liquid seat and be the awl bucket shape, the bottom of going out the liquid seat is equipped with the liquid outlet is equipped with the rotary switch who is used for switching liquid outlet.

Further, a disposable fecal sample processor still include out the liquid seat, go out the liquid seat and connect the bottom of second filter seat, go out the liquid seat and link up from top to bottom, go out the liquid seat and be the awl bucket shape, the bottom of going out the liquid seat is equipped with the liquid outlet is equipped with the rotary switch who is used for switching liquid outlet.

Further, the second filter layer comprises an upper filter screen, a plurality of immunoadsorption magnetic beads and a lower filter screen, a space for containing the immunoadsorption magnetic beads is formed between the upper filter screen and the lower filter screen, the lower filter screen is fixedly connected with the second filter seat, and the upper filter screen is detachably connected with the second filter seat.

Furthermore, the side wall of the sample container is provided with 1-10ml of scale marks, and the precision of the scale marks is 0.1ml plus or minus 0.05ml.

Further, the cross section of the buckle is inverted saw-tooth-shaped.

Further, the shape of the liquid dropping mouth is conical, the length is 1.5cm, and the diameter of the outlet is 0.5-1.0mm.

After the technical scheme is adopted, the utility model has the following advantages:

after the excrement is sampled and added into the sample container, the disposable excrement sample processor can be used for preserving, delivering and preprocessing samples, such as vibration, shaking, ultrasound and the like, and does not generate leakage; according to the disposable fecal sample processor disclosed by the utility model, the sealing guide seat is pushed to enable the sealing guide seat to move outwards relative to the sample container until the buckle enters the second clamping groove, so that a sample outlet at the bottom of the sample container can be opened, and the sample container is connected with the first filtering seat and the second filtering seat to realize filtering of samples.

In conclusion, the disposable fecal sample processor provided by the utility model simplifies the operation steps and improves the working efficiency through the integrated flow design. The disposable fecal sample processor disclosed by the utility model has the advantages that the sample filtering treatment and sample adding are closed, other objects are not required to be contacted, the risk of leakage and human body and environmental pollution is avoided, the pollution to personnel and the environment is avoided, and the safety is higher.

Drawings

FIG. 1 is a schematic diagram of a first combination of a disposable fecal sample processor;

FIG. 2 is a schematic diagram of a disposable fecal sample processor of a second combination;

FIG. 3 is a schematic diagram of a third combination of a disposable fecal sample processor;

fig. 4 is a bottom view of the seal deflector.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1-4, a disposable fecal sample processor comprising: the device comprises a sealing cover 1, a sample container 2, a sealing flow guiding seat 3, a first filtering seat 4, a second filtering seat 6 and a liquid outlet seat 5.

The sample container 2 is made of an environment-friendly degradable plastic material, 1-10ml of scale marks are arranged on the side wall of the sample container 2, and the precision of the scale marks is 0.1ml. The sample container 2 is cylindrical in shape, preferably cylindrical, as appropriate for assembly and handling.

The top of the sample container 2 is provided with a sample adding port 201, and the sealing cover 1 is connected to the sample adding port 201 of the sample container 2. The connection mode of the sealing cover 1 and the sample container 2 may be: screw connection, clamping connection and the like, as long as the sealing cover 1 and the sample container 2 can be connected without liquid leakage.

The bottom of the sample container 2 is provided with a sample outlet 202, and the shape of the sample outlet 202 is a frustum shape gradually widened from inside to outside of the sample container 2. The sealing guide seat 3 is columnar with an opening at the top end, and the sealing guide seat 3 is sleeved outside the sample container 2. The bottom of the sealing flow guiding seat 3 is provided with a sealing plug 301 which is matched with the shape of the sample outlet 202, and the sealing plug 301 is made of rubber material. As shown in fig. 4, the sealing diversion seat 3 is provided with a plurality of sample passing openings 302 along the circumferential direction at the periphery of the sealing plug 301.

The top of the inner wall of the sealed flow guiding seat 3 is provided with a buckle 303, and the outer wall of the sample container 2 is provided with a first clamping groove 203 and a second clamping groove 204 corresponding to the buckle 303. The cross section of the buckle 303 is inverted saw-tooth.

When the buckle 303 is located in the first clamping groove 203, the sealing plug 301 is tightly attached to the side wall of the sample outlet 202, and the sample outlet 202 is closed; pulling the sealing guide seat 3 to enable the sealing guide seat 3 to move outwards relative to the sample container 2 until the buckle 303 enters the second clamping groove 204, at this time, the sealing plug 301 is separated from the side wall of the sample outlet 202, a sample outlet flow channel is formed between the sealing plug 301 and the side wall of the sample outlet 202, and a sample enters the sample passing port 302 after passing through the sample outlet flow channel.

The buckle 303 with the inverted saw-tooth-shaped cross section enables the buckle 303 to not retract from the second clamping groove 204 to the first clamping groove 203, so that after the buckle 303 enters the second clamping groove 304, the container containing the sample, which is composed of the sealing cover 1, the sample container 2 and the sealing guide seat 3, cannot be reused and needs to be discarded.

The height of the sealing plug 301 is higher than the height of the sample outlet 202, at least so that the top of the sealing plug 301 is higher than the inner bottom surface of the sample container 2 when the catch 303 is located in the second catch groove 204. Thus, the samples are annularly distributed at the bottom of the sample container 2, and the samples can smoothly flow to the sample outlet flow channel.

The first filter seat 4 is connected to the bottom end of the sealing guide seat 3. The connection manner of the first filtering seat 4 and the sealing diversion seat 3 may be: threaded connection, clamping connection and the like, as long as the first filter seat 4 and the sealing guide seat 3 can be connected without liquid leakage.

The first filter seat 4 is vertically penetrated, and a first filter layer 401 is arranged inside the first filter seat 4. The first filtering layer 401 is a filter screen or a filter membrane, and is used for preventing insoluble particulate matters with the diameter larger than 10 mu M from passing through. The filter screen is a glass sand or glass microsphere or glass fiber filter screen, and the filter screen is a cellulose filter screen.

The first filter layer 401 arrangement is typically a cone-shaped arrangement or a horizontal arrangement. Even when the first filter layer 401 is horizontally arranged, a situation in which the middle of the first filter layer 401 is depressed occurs under the action of the gravity of the sample, resulting in uneven distribution of the sample on the first filter layer 401. In this embodiment, the sample passing ports 302 are disposed in the circumferential direction of the sealing plug 301, so that samples flow from the position of the first filter layer 401 near the outer edge to the middle of the first filter layer 401, so that the samples are uniformly distributed on the first filter layer 401, the filtering efficiency of the first filter layer 401 is increased, and the filtering effect of the first filter layer 401 is improved.

The second filter seat 6 is connected to the bottom end of the first filter seat 4. The connection manner between the second filter base 6 and the first filter base 4 may be: threaded connection, clamping connection and the like, as long as the second filter seat 6 and the first filter seat 4 can be connected without liquid leakage.

The second filter seat 6 is vertically communicated, and a second filter layer is arranged in the second filter seat 6. The second filter layer is an immunoadsorption microplate or magnetic beads and is used for adsorbing specific DNA, RNA, antigens and the like. In this embodiment, the second filter layer includes an upper filter 601, a plurality of immunoadsorption magnetic beads 602, and a lower filter 603, and a space for accommodating the plurality of immunoadsorption magnetic beads 602 is formed between the upper filter 601 and the lower filter 603. The upper screen 601 and the lower screen 603 also have the function of preventing the passage of insoluble particulate matter having a diameter greater than 10 μm. The lower filter screen 603 is fixedly connected with the second filter seat 6, and the upper filter screen 601 is detachably connected with the second filter seat 6, so that the immunoadsorption magnetic beads 602 can be conveniently taken and placed.

The liquid outlet seat 5 is connected to the bottom end of the first filtering seat 4 or the second filtering seat 6. The connection mode between the liquid outlet seat 5 and the first filtering seat 4/the second filtering seat 6 may be: threaded connection, clamping connection and the like, as long as the liquid outlet seat 5 and the first filter seat 4/the second filter seat 6 can be connected without liquid leakage.

The liquid outlet seat 5 is vertically communicated, the liquid outlet seat 5 is in a cone bucket shape, and a liquid drop port 501 is arranged at the bottom of the liquid outlet seat 5. The drip port 501 is preferably conical in shape, 1.5cm in length and 0.5-1.0mm in diameter at the outlet, ensuring that the size of each drip of filtered liquid is 30+ -1.5 μl. The drip port 501 is provided with a rotary switch 7 for opening and closing the drip port 501.

The utility model relates to a disposable fecal sample processor, which has three combination forms:

the first combination, as shown in figure 1, consists of a sealing cover 1, a sample container 2 and a sealing flow guiding seat 3. The buckle 303 of the sealing diversion seat 3 is positioned in the first clamping groove 203 of the sample container 2. The first combination is used for sample preservation and delivery and pretreatment, such as shaking, ultrasound, etc., without leakage.

The second combination, as shown in figure 2, consists of a sealing cover 1, a sample container 2, a sealing flow guiding seat 3, a first filtering seat 4 and a liquid outlet seat 5. At this time, the buckle of the sealing diversion seat 3 is positioned in the second clamping groove 204 of the sample container 2. The second combination was used for filtration of the samples.

The third combination, as shown in figure 3, consists of a sealing cover 1, a sample container 2, a sealing flow guiding seat 3, a first filtering seat 4, a second filtering seat 6 and a liquid outlet seat 5. At this time, the buckle 303 of the sealing diversion seat 3 is positioned in the second clamping groove 204 of the sample container 2. The third combination was used for DNA, RNA, antigen adsorption of the samples.

The following illustrates an application scenario of a disposable fecal sample processor of the present utility model:

application scenario one: fecal occult blood test (chemical or colloidal gold method).

A disposable fecal sample processor is used for the treatment and loading of fecal samples in clinical assays. The implementation method comprises the following steps: one gram of excrement is taken by a sampler (by using a quantitative sampler), the sealing cover 1 is opened, the excrement is put into a sample container 2 filled with 9ml of excrement sample dissolving liquid (timely or pre-filled), the sealing cover 1 is covered, and the excrement sample dissolving liquid is uniformly mixed. Holding or supporting frame keeps upright position, after installing first filter seat 4 and play liquid seat 5, aim at the application of sample window of test paper, open sealed lid 1 in top, promote sealed water conservancy diversion seat 3 downwards, then rotatory rotation switch 7 of opening out liquid seat 5 bottom, liquid drips out to the application of sample window of test paper from dropping liquid mouth 501 behind first filter seat 4, after two drips (according to test paper specification if certain) rotatory rotation switch 7 closes dropping liquid mouth 501, the application of sample is accomplished.

And (2) an application scene II: pretreatment of fecal gene detection test samples.

A disposable fecal sample processor is used for the treatment and loading of fecal samples in clinical assays. The implementation method comprises the following steps: one gram of excrement is taken by a sampler (by using a quantitative sampler), the sealing cover 1 is opened, the excrement is put into a sample container 2 filled with 9ml of excrement sample dissolving liquid (timely or pre-filled), the sealing cover 1 is covered, and the excrement sample dissolving liquid is uniformly mixed. The hand-held or stand is kept in an upright position, after the first filter seat 4 and the liquid outlet seat 5 are installed, the liquid collector is aligned, the top sealing cover 1 is opened, the sealing diversion seat 3 is pushed downwards, then the rotary switch 7 at the bottom of the liquid outlet seat 5 is rotated and opened, liquid is dripped into the collector from the liquid dripping port 501 after passing through the first filter seat 4, and the maximum collection amount is estimated to be 9ml. The collected liquid is the fecal dissolving liquid after treatment and filtration, and is used for further separation, purification, extraction or detection (according to the detection method).

And (3) an application scene III: adsorption filtration-fecal sample DNA adsorption.

A disposable fecal sample processor for the adsorption of specific substances (DNA) in a fecal sample. The implementation method comprises the following steps: one gram of excrement is taken by a sampler (by using a quantitative sampler), the sealing cover 1 is opened, the excrement is put into a sample container 2 filled with 9ml of excrement sample dissolving liquid (timely or pre-filled), the sealing cover 1 is covered, and the excrement sample dissolving liquid is uniformly mixed. The hand or the support keeps the upright position, after the first filtering seat 4, the second filtering seat 6 and the liquid outlet seat 5 are installed, the liquid collector is aligned, the top sealing cover 1 is opened, then the rotary switch 7 at the bottom of the liquid outlet seat 5 is rotated to open, and the liquid is dripped into the collector from the liquid dripping port 501 after passing through the first filtering seat 4 and the second filtering seat 6. The DNA in the sample is retained in the second filter holder 6 for further elution, separation and purification, gene detection, and the like.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims

1. The utility model provides a disposable fecal sample treater, its characterized in that, includes sealed lid, sample container, sealed guide seat, first filter seat, sample container's top is equipped with the application of sample mouth, sealed lid is connected sample container's application of sample mouth department, sample container's bottom is equipped with out the appearance mouth, the shape of appearance mouth is by the interior taper frustum shape that gradually widens outward of sample container, sealed guide seat is open-ended column, sealed guide seat suit is in sample container is outer, sealed guide seat's bottom be equipped with the sealing plug that appearance mouth shape suited, sealed guide seat is in the periphery of sealing plug is equipped with a plurality of sample mouths in circumference, sealed guide seat inner wall is equipped with the buckle be equipped with on sample container's the outer wall with the corresponding first draw-in groove of buckle and second draw-in groove, first filter seat is connected the bottom of sealed guide seat, first filter seat link up from top to bottom, the inside of first filter seat is equipped with first filter layer.

2. The disposable fecal sample processor according to claim 1, further comprising a second filter holder, wherein the second filter holder is connected to the bottom end of the first filter holder, the second filter holder is vertically penetrated, and a second filter layer for adsorbing specific DNA, RNA and antigen is provided inside the second filter holder.

3. The disposable fecal sample processor according to claim 1, further comprising a liquid outlet seat, wherein the liquid outlet seat is connected to the bottom end of the first filter seat, the liquid outlet seat is vertically through, the liquid outlet seat is in a cone bucket shape, a liquid drop port is arranged at the bottom of the liquid outlet seat, and a rotary switch for opening and closing the liquid drop port is arranged at the liquid drop port.

4. The disposable fecal sample processor according to claim 2, further comprising a liquid outlet seat, wherein the liquid outlet seat is connected to the bottom end of the second filter seat, the liquid outlet seat is vertically through, the liquid outlet seat is in a cone bucket shape, a liquid drop port is arranged at the bottom of the liquid outlet seat, and a rotary switch for opening and closing the liquid drop port is arranged at the liquid drop port.

5. The disposable fecal sample processor according to claim 2, wherein the second filter layer comprises an upper filter screen, a plurality of immunoadsorption magnetic beads, a lower filter screen, wherein a space for accommodating the plurality of immunoadsorption magnetic beads is formed between the upper filter screen and the lower filter screen, the lower filter screen is fixedly connected with the second filter seat, and the upper filter screen is detachably connected with the second filter seat.

6. A disposable fecal sample processor according to claim 1, wherein the side wall of the sample container is provided with 1-10ml graduation marks with an accuracy of 0.1ml ± 0.05ml.

7. The disposable fecal sample processor according to claim 1, wherein the cross section of the clasp is inverted saw-tooth shaped.

8. A disposable fecal sample processor according to claim 3 or 4, wherein the drip opening is conical in shape with a length of 1.5cm and an outlet diameter of 0.5-1.0mm.