CN108925557A - Acetaldehyde is preparing the application in nematode killer - Google Patents
Acetaldehyde is preparing the application in nematode killer Download PDFInfo
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- CN108925557A CN108925557A CN201810714260.3A CN201810714260A CN108925557A CN 108925557 A CN108925557 A CN 108925557A CN 201810714260 A CN201810714260 A CN 201810714260A CN 108925557 A CN108925557 A CN 108925557A
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- nematode
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- knot nematode
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
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Abstract
The invention discloses the active volatile materials acetaldehyde of a kind of pair of root-knot nematode.Acetaldehyde has very strong contact toxicity to Nematicidal Activity as the result is shown, and half lethal concentration is respectively EC50/6h (mg/L)=141.4 μ g/ml；It has fumigation activity to root-knot nematode；There is inhibiting effect to root-knot nematode egg hatching；Chemotaxis test shows that it has attracting action to root-knot nematode.Acetaldehyde is generally as insect attractant and fumigant, and to the insecticidal action of root-knot nematode, inhibiting effect and attracting action to egg hatching are to find for the first time.
Description
Technical field
The invention belongs to technical field of agricultural microbiology, and in particular to acetaldehyde is preparing the application in nematode killer.
Technical background
Root-knot nematode (Meloidogyne incognita；RKNs it is) exclusive root parasitism worm, worldwide infects
More than 3000 kinds plant species, this is one of the plant nematode of most serious in the world, especially in Perenniporia martius
Agricultural district (Kong Xiangyi etc. 2006).Root-knot nematode (Meloidogyne spp.) is a kind of endoparasitism being colonized in root system of plant
Nematode belongs to Tylenchida (Tylenchida), Heteroderidae (Heteroderidae), root knot subfamily (Meloidogyninae), root
Tie lines Eimeria (Meloidogyne).In China, distribution is most wide, the most serious is Meloidogyne incognitas for harm.Root-knot nematode is sometimes
Highly difficult control is waited, since its host range is extensive, life cycle is short, and breeding potential is high, and endoparasitism is good (Chen etc. 2004).
Prevention and treatment for root-knot nematode plays in ensureing higher yield of crops stable yields always based on chemical agent
Important function.But but due to its toxic residue, nematocide environmental pollution is serious, also uneasy to people and animals in use process
Entirely.Many nematode killing agents are disabled, many developing countries, especially the torrid zone or subtropical zone, and weather is suitable for nematode
Activity and breeding, perennial plant and the crop planted year after year in same piece of land, when be subjected to nematode harm very serious,
And the quantity of food that developing country needs is very big, it is necessary to rely on perennial crop and continuous cropping, therefore seldom anti-using crop rotation
Control root-knot nematode；The application of resistant variety is not also extensive at present, first is that due to being limited by economical character, second is that due on a ground
After a disease-resistant variety is continuously planted in area, the selection index system of disease-resistant variety makes the group of nematode change, and makes the kind not
The quantity of anti-a small number of nematode types increases, to gradually lose resistance.Due to above, people are some high except continuing to explore
Effect, low toxicity, low-residual outside highly selective nematode killing agent, should also seek new method, and biological control is wherein from ecological view
One of (Wang Laifa etc. 2002).
In recent years, the Volatile Metabolites that microorganism generates gradually show potential in terms of plant nematode prevention and treatment
Value.Volatile organic compounds (VOC), including alcohol, aldehyde, ketone, alkene, ether, phenols, esters etc., which much all have, well kills
Nematode activity.The characteristic that there is volatile organic compounds general nematocide agent not have: some volatile organic compounds
Plant resistance to environment stress can be improved by the activation of plant defense signal path；Its good soil permeability, volatility keep it straight
Up to cause of disease, plant disease can be more effectively killed；Microorganism generally generates a variety of volatile organic compounds, avoids plant
The drug resistance that disease generates；In addition, volatile organic compounds is generally also provided with and lures or repellent pathogenic nematode and the work of insect
With.
Have now been found that the volatile organic compounds (VOC) for much having eelworm-killing activity, representative is stifling
Agent has halogenated hydrocarbon and thiocarbimide methyl esters class, including bromomethane, iodomethane, vikane, dazomet, metham-sodium, 1,3- dichloro
Propylene, chloropicrin, nitrolim, methyl-isorhodanate, dimethyl disulfide etc. (Lu Zhijun etc. 2016).Volatile organic compounds
Research in terms of nematode lures repellant is less, and nematode lures repellant to have gaultherolin, lauric acid etc..
Summary of the invention
It includes preparation that the object of the present invention is to provide acetaldehyde preparing the application in nematode killer, the application
At insecticide, fumigant and attractant, the acetaldehyde chemical formula is as follows:
In order to reach the goals above, the present invention uses following technical measures:
Acetaldehyde includes using acetaldehyde as main effect component or effective component preparing the application in nematode killer, application process
One of be prepared into nematode killer or nematode fumigant or nematode attractant or nematode ovoinhibitor.
Above-described application, it is preferred that the nematode is Meloidogyne incognita.
Compared with prior art, the invention has the following advantages that
The present invention reports the root-knot nematode attractive activity of acetaldehyde, the killing root-knot nematode activity of acetaldehyde, the suppression of acetaldehyde for the first time
Egg hatching fumigation activity processed provides new selectivity for the preparation of novel root-knot nematode biological prevention and control agent.
The nematode killing agent of acetaldehyde preparation has the advantages that efficient, low toxicity, duration are good, has a variety of binding modes.
Detailed description of the invention
Fig. 1: the desinsection figure of acetaldehyde killing root-knot nematode；
Wherein: A: control group nematode；B: acetaldehyde processing group nematode (for 24 hours).
Fig. 2: acetaldehyde inhibits the desinsection figure of nematode by fumigation action；
Wherein: A: control group nematode；B: acetaldehyde processing group nematode (for 24 hours).
Fig. 3 is Chemotaxis test schematic diagram.
Specific embodiment
Experimental method in following embodiments is unless otherwise instructed the microbiology conventional practices of report.Institute
Reagent or material are stated, if not otherwise specified, derives from commercial channel.
Embodiment 1:
Application of the acetaldehyde in preparation meloidogyne pesticide, application process are as follows:
From tomato root picking nematode infections root knot, pieces of an egg are placed in 20 DEG C of 96 orifice plate incubations, observation root-knot nematode hatching
Situation；It collects root-knot nematode J2 phase larva and is in control nematode suspension in centrifugation；It is loaded 200 μ l in 96 orifice plates, is with solvent
Blank control, is added 2 μ L chloramphenicol (30mg/mL) and 30 nematodes, every group of 3 repetitions, and sealed membrane sealing is placed in constant temperature incubation
Case；In 6h, for 24 hours observation experiment result.
Nemic death rate=nematode death toll/for trying nematode number * 100%
1. acetaldehyde of table kills Meloidogyne incognita activity
Embodiment 2:
Application of the acetaldehyde in preparation root-knot nematode fumigant, application process are as follows:
From tomato root picking nematode infections root knot, pieces of an egg are placed in 20 DEG C of 96 orifice plate incubations, observation root-knot nematode hatching
Situation；It collects root-knot nematode J2 phase larva and is in control nematode suspension in centrifugation；A hole is selected to be loaded 200 μ in 96 orifice plates
L, closely 2 μ L chloramphenicol (30mg/mL) and 60 nematodes are added in the four of the hole hole, each hole, and sealed membrane sealing is placed in constant temperature
Incubator；In 6h, for 24 hours observation experiment result.
Nemic death rate=nematode death toll/for trying nematode number * 100%
2. acetaldehyde of table kills Meloidogyne incognita fumigation activity
10mg/mL acetaldehyde fumigates root-knot nematode, and the 6h death rate reaches 100%.
Embodiment 3:
Acetaldehyde fumigates Inhibition test to Meloidogyne incognita egg hatching
From tomato root picking nematode infections root knot, pieces of an egg are placed in 20 DEG C of 96 orifice plate incubations, observation root-knot nematode hatching
Situation；It collects root-knot nematode J2 phase larva and is in control nematode suspension in centrifugation；A hole is selected to be loaded 200 μ in 96 orifice plates
L, closely 1 egg capsule is added in the four of the hole hole, each hole, and sealed membrane sealing is placed in constant incubator；It is observed in 1d, 2d, 3d
Experimental result.
Inhibiting effect of 3. acetaldehyde of table to Meloidogyne incognita egg hatching
10mg/mL acetaldehyde fumigates root-knot nematode egg capsule, and the hatching of ovum can be effectively suppressed.
Embodiment 4:
Acetaldehyde prepares the application of the attractant of Meloidogyne incognita, and application process is as follows:
The root-knot nematode of hatching is collected in centrifuge tube, obtains J2 nematode suspension；Sample solution is configured, and is diluted to
10mg/mL, 3mg/mL, 1mg/mL；By sample-adding product shown in Chemotaxis test schematic diagram (Fig. 3), nematode is added in plate center and suspends
Liquid contains nematode 100；Plate is sealed with sealed membrane, is placed in 20 DEG C of insulating boxs, shading, 8h is observed and counted the experiment of buffer area two sides
The nematode number in area and check plot.
Chemotactic index (C.I value)=(test block nematode number-check plot nematode number)/(test block nematode number+check plot nematode
Number)
C.I ∈ (0,1] indicate attracting action
C.I ∈ [- 1,0) indicate repellent action
Attracting action of 4. acetaldehyde of table to Meloidogyne incognita
C.I value is all larger than 0, and acetaldehyde has sucking action to root-knot nematode.
Claims (4)
1. acetaldehyde is preparing the application in nematode killer.
2. application of the acetaldehyde in preparation nematode attractant.
3. application of the acetaldehyde in preparation nematode fumigant.
4. acetaldehyde is preparing the application in line eggs inhibitor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810714260.3A CN108925557B (en) | 2018-07-03 | 2018-07-03 | Application of acetaldehyde in preparation of nematode insecticide |
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| CN201810714260.3A CN108925557B (en) | 2018-07-03 | 2018-07-03 | Application of acetaldehyde in preparation of nematode insecticide |
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| CN108925557A true CN108925557A (en) | 2018-12-04 |
| CN108925557B CN108925557B (en) | 2020-10-16 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL77330C (en) * | ||||
| CN102027901A (en) * | 2010-12-07 | 2011-04-27 | 浙江农林大学 | Adult monochamus alternatus hope attractant |
| CN103299999A (en) * | 2013-06-26 | 2013-09-18 | 中国农业科学院植物保护研究所 | Application technology of furfural serving as soil fumigant |
| CN104412968A (en) * | 2013-09-06 | 2015-03-18 | 中国农业大学 | Application of vanillic aldehyde in preparation of Meloidogyne incognita Chitwood insecticide inhibitor |
| CN107047621A (en) * | 2017-06-14 | 2017-08-18 | 福建农林大学 | Gentamicin phenylacetaldehyde mixture nematicide and preparation method thereof |
-
2018
- 2018-07-03 CN CN201810714260.3A patent/CN108925557B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL77330C (en) * | ||||
| CN102027901A (en) * | 2010-12-07 | 2011-04-27 | 浙江农林大学 | Adult monochamus alternatus hope attractant |
| CN103299999A (en) * | 2013-06-26 | 2013-09-18 | 中国农业科学院植物保护研究所 | Application technology of furfural serving as soil fumigant |
| CN104412968A (en) * | 2013-09-06 | 2015-03-18 | 中国农业大学 | Application of vanillic aldehyde in preparation of Meloidogyne incognita Chitwood insecticide inhibitor |
| CN107047621A (en) * | 2017-06-14 | 2017-08-18 | 福建农林大学 | Gentamicin phenylacetaldehyde mixture nematicide and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| CAPLUS: "Effect of acetaldehyde on free living soil nematodes Caenorhabditis elegans, N2- and IPE1-lines", 《STN CAPLUS 摘要 159:650734》 * |
| CHRISTIE, J. R. ET.AL.: "Testing the efficacy of chemicals for killing soil-inhabiting nematodes under field conditions", 《PROC. HELMINTHOL. SOC. WASH., D.C.》 * |
| DIAN HUANG ET.AL.: "Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493", 《MOLECULES》 * |
| TERRILL, WILLLIAM F. ET.AL.: "Threshold chemosensitivity and hypothetical chemoreceptor function of the nematode Caenorhabditis elegans", 《JOURNAL OF CHEMICAL ECOLOGY》 * |
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