CN101984828B - Compound pesticide - Google Patents
Compound pesticide Download PDFInfo
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- CN101984828B CN101984828B CN 201010524617 CN201010524617A CN101984828B CN 101984828 B CN101984828 B CN 101984828B CN 201010524617 CN201010524617 CN 201010524617 CN 201010524617 A CN201010524617 A CN 201010524617A CN 101984828 B CN101984828 B CN 101984828B
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- green muscardine
- nimbin
- muscardine fungus
- stabilizing agent
- cosolvent
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Abstract
The invention discloses a compound pesticide, which is mainly prepared from the following raw materials in percentage by weight: 5-20% of azadirachtin and metarrhizium, 8-12% of emulsifier, 3-7% of stabilizer, 2-8% of synergist, 0.1-1.0% of antioxidant, 10-18% of cosolvent and 50-60% of organic solvent. The compound pesticide is environment-friendly, has high compatibility and obvious synergistic effect, and can obviously improve the insecticidal toxicity of a single dosage and reduce the use of high-persistent chemical pesticide.
Description
Technical field
The present invention relates to a kind of compound biotic insecticide, be specifically related to a kind of green muscardine fungus and azadirachtin complex insecticide.
Background technology
Green muscardine fungus is a kind of insect pathogenic fungus of broad spectrum activity, is widely used in multiple agriculture, woods, herds, the control of sanitary insect pest.Research and the application green muscardine fungus control agriculture and forestry injurious insect history of existing more than 100 year.Over nearly 20 years, research is all over the world used the green muscardine fungus pest control and is reached kind more than 300, approximately has 100 various pests to carry out field controling test, and from the basic field that the scale of preventing and treating and research relate to, green muscardine fungus has become the fungus insecticide that is only second to white muscardine fungi.Green muscardine fungus can be used for the soil insect in nursery in forestry, the trees termite, the biological control of trunk borer and defoliator, can be used for the grub control of the soil insect, paddy rice froghopper, peanut, sugarcane etc. of pollution-free vegetable on agricultural, can be used for the biological control of herbage locust and soil insect in livestock breeding.But because of shortcomings such as fungus insecticide have that control efficiency is unstable, desinsection speed is slow and cost is relatively high, be subject to certain limitation on using.
Nimbin is as a kind of efficient botanical pesticide, it is the insecticide that wide spectrum recognized in the world, efficient, low toxicity, easily degraded, noresidue, target pest are difficult for developing immunity to drugs, nearly all plant insect is all had repelling and killing efficacy, and to people and animals and surrounding environment without any pollution.To target pest have food refusal, repellent, inhibition growth, disturb lay eggs, the effects such as interference variation, effectively Control pests causes harm.Proved various pests tool insecticidal action, can prevent and treat more than 200 kind of agriculture, woods, storage and sanitary insect pest, especially the insects such as Lepidoptera, coleoptera and diptera have been had special efficacy.
Since entering the chemical insect protected epoch, people more and more come by force the dependence of chemical pesticide, the long-term use of some chemical chemical insecticide, increased insect pesticide resistance, polluted environment.Multiple mixed insecticide is arranged in the market, mostly be being mixed between the chemical pesticide of different mechanism of action, its usage amount is large, and insect easily develops immunity to drugs.The long-term use of various chemical medicaments has caused the increase of agricultural land used persticide residue, cause multiple product to be concluded the business in the international market limited, and insect to the drug-fast increase of medicament and medicament to the increase that also causes dosage and medicine frequency that kills and wounds of natural enemy insect, usually cause the generation again of a certain insect.
For the insecticidal rate that improves fungus insecticide, reduce use cost, with fungus insecticide and the composite use of compatibility chemical pesticide, but exist the compatibility medicament on problems such as the impact of fungal spore vigor in built agent and chemical pesticide environmental pollutions in production.
Summary of the invention
The object of the invention is to provide a kind of compounded pesticides, this insecticide and environmental friendliness, can enlarge biopesticide insecticidal spectrum, increase insecticidal effect, reduce the use of high residue chemical pesticide.
A kind of compounded pesticides provided by the invention, it is mainly made by the raw material of following weight percentage:
Nimbin and green muscardine fungus 5-20%
Emulsifier 8-12%
Stabilizing agent 3-7%
Synergist 2-8%
Antioxidant 0.1-1.0%
Cosolvent 10-18%
Organic solvent 50-60%.
The weight part ratio of green muscardine fungus of the present invention and nimbin is 25-99: 1.
Wherein, nimbin and entomogenous fungi are compatible, and have different desinsection modes and desinsection object, both carry out the compound new compounded pesticides that obtains, and can enlarge desinsection general, improve insecticide efficiency.Prepared insecticide has the brand-new mechanism of action that is different from existing insecticide, and insecticidal effect is remarkable, and is especially remarkable for the serious lepidoptera pest toxic action of harm, has simultaneously eco-friendly characteristics.
Green muscardine fungus of the present invention is the conidium that solid fermentation obtains, the high cryptogam that obtains after extracting, and its weight metering mode is the spore content (spore/g) of built agent endoconidium total content/pure conidial powder.
Emulsifier of the present invention is preferably tween, many sorbitol esters, sodium alkyl sulfate, NaLS or alkylbenzenesulfonate.
Stabilizing agent of the present invention is preferably epoxidized soybean oil or epoxidized linseed.
Synergist of the present invention is preferably Butacide, chlorination fat, its ester of carbonic acid alkane or sulfoxide.
Antioxidant of the present invention is preferably one or more in butyl alkyl anisole, dibutyl toluene, n-propyl gallate and dilauryl thiodipropionate.
Cosolvent of the present invention is preferably one or more in isoamyl alcohol, phenol, ethyl acetate and dimethyl sulfoxide (DMSO).
Organic solvent of the present invention is preferably dimethylbenzene.
The preparation method of above-mentioned compounded pesticides is as follows: take the high cryptogam of green muscardine fungus and nimbin raw medicine by above-mentioned metering ratio, first nimbin raw medicine is dissolved in the solvent that contains Partial Antioxidation agent and stabilizing agent, quantitatively add again the high cryptogam of green muscardine fungus, emulsifier, synergist and cosolvent, airtight stirring 10-15min, add again residue antioxidant, stabilizing agent and organic solvent, and be adjusted to aimed concn, and continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Compared with prior art, the present invention has following advantage:
1.. nimbin and muscardine spore have good compatibility, and synergistic effect is obvious, can obviously improve the insecticidal toxicity of each single dose;
2.. production technology is simple, can significantly reduce the usage amount of agricultural chemicals, delays the pesticide resistance of insect, and insect is easy to form epidemic disease, successful in the non-environmental pollution control of insect and lasting integrated control;
3.. to be that host is composite by two kinds of Biocidal single doses form composite insecticide, the compatibility good with the environment tool, extensive use to agricultural production and income, development environmental protection agricultural, improve the ecological environment, improve level of human health, realize that the strategy of sustainable development is significant.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment, and following reagent and raw material are commercially available if no special instructions.
Embodiment 1
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 10%, and the weight proportion of each raw material is as follows:
Nimbin 0.2g
Green muscardine fungus 9.8g (15,000,000,000 spores/g, lower same)
Emulsifier 10g
Stabilizing agent 5g
Synergist 5g
Antioxidant 0.5g
Cosolvent 14.5g
Organic solvent 55g.
Wherein the high cryptogam of green muscardine fungus can obtain (lower same) in the following way: with the green muscardine fungus bombys batryticatus that gathers, through indoor separating and preserving, through Toxicity Determination, filter out the Metarhizium Strains that several forestry pests is had high virulence, for the production of the muscardine spore powder.Adopt liquid-solid biphasic fermentation to cultivate, utilize closed fungus insecticide solid fermentation culture bag, the good fermenting green muscardine fungus liquid of inoculated and cultured in solid culture medium (rice), seal cultivation, open bag and cultivate after solid culture medium covers with mycelia, dry negative pressure extraction wait producing spore after fully, get the high cryptogam of green muscardine fungus, perhaps directly buy commercially available muscardine spore powder.
The preparation process of above-mentioned formula insecticide is: accurately take the high cryptogam of green muscardine fungus and nimbin raw medicine, first nimbin raw medicine is dissolved in the solvent that contains Partial Antioxidation agent butyl alkyl anisole, cosolvent isoamyl alcohol and stabilizing agent epoxidized soybean oil, add again the high cryptogam of green muscardine fungus, emulsifier tween, synergist Butacide, airtight stirring 10-15min, add again residue antioxidant, stabilizing agent and organic solvent dimethylbenzene, and be adjusted to aimed concn, continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Embodiment 2
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 8%, and the weight proportion of each raw material is as follows:
Azadirachtin Cotent 0.2g
Green muscardine fungus content 7.8g
Emulsifier 10g
Stabilizing agent 6g
Synergist 6g
Antioxidant 0.5g
Cosolvent 14.5g
Organic solvent 55g.
The preparation process of above-mentioned formula insecticide suspending agent is: accurately take the high cryptogam of green muscardine fungus and nimbin raw medicine, first nimbin raw medicine is dissolved in the solvent that contains Partial Antioxidation agent n-propyl gallate and stabilizing agent epoxidized soybean oil, add again the high cryptogam of green muscardine fungus, the many sorbitol esters of emulsifier, synergist chlorination fat and cosolvent phenol, airtight stirring 10-15min, add again residue antioxidant, stabilizing agent and organic solvent dimethylbenzene, and be adjusted to aimed concn, continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Embodiment 3
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 15%, and the weight proportion of each raw material is as follows:
Azadirachtin Cotent 0.3g
Green muscardine fungus content 14.7g
Emulsifier 8g
Stabilizing agent 4g
Synergist 4g
Antioxidant 0.5g
Cosolvent 13.5g
Organic solvent 55g.
The preparation process of above-mentioned formula insecticide suspending agent is: accurately take the high cryptogam of green muscardine fungus and nimbin raw medicine, first nimbin raw medicine is dissolved in the solvent that contains Partial Antioxidation agent dilauryl thiodipropionate and stabilizing agent epoxidized linseed, add again the high cryptogam of green muscardine fungus, emulsifier sodium alkyl sulfate, synergist sulfoxide and cosolvent ethyl acetate, airtight stirring 10-15min, add again residue antioxidant, stabilizing agent and organic solvent dimethylbenzene, and be adjusted to aimed concn, continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Embodiment 4
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 5%, and the weight proportion of each raw material is as follows:
Nimbin 0.19g
Green muscardine fungus 4.81g
Emulsifier 12g
Stabilizing agent 3g
Synergist 8g
Antioxidant 0.1g
Cosolvent 18g
Organic solvent 50g.
Its preparation method is with embodiment 1-3.
Embodiment 5
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 20%, and the weight proportion of each raw material is as follows:
Nimbin 0.4g
Green muscardine fungus 19.6g
Emulsifier 8g
Stabilizing agent 7g
Synergist 2g
Antioxidant 1.0g
Cosolvent 10g
Organic solvent 60g.
Its preparation method is with embodiment 1-3.
The Toxicity Determination of compounded pesticides of the present invention and control effect in forest:
One, the Toxicity Determination effect of compounded pesticides
Reagent agent: green muscardine fungus-nimbin suspending agent (the Ma985 bacterial strain of green muscardine fungus for snout moth is had high virulence, green muscardine fungus and nimbin weight ratio are 98: 2, green muscardine fungus content is 10,000,000,000 spores/ml); For trying insect: oranges and tangerines long paper moth 3 instar larvaes of harm Aegiceras corniculatum.Gather the 3-4 paulownia oranges and tangerines long paper moth larvae in age from woodland, indoor feeding 2-3 days, select the larva of bouncing, put into plastics square box (17cm * 17cm), 3 repetitions of every processing, 10 larvas of every repetition.With 1000 times of institute's dose out powders dilutions, be sprayed to blade surface, 26 ± 1 ℃, raise under 80 ± 1%RH condition, after one day, the blade that is replaced by without medicine continues to raise, and compares with the distilled water that sprays with amount.See examination worm death condition every day, and change fresh blade, in one week of Continuous Observation, add up the corrected mortality of each processed group.
Table 1 green muscardine fungus and nimbin mixed toxicity test to oranges and tangerines long paper moth
Two, compounded pesticides control effect in forest:
The Field efficacy tests point is selected in high bridge protection station, country Mangrove Nature Reserves Lianjiang county, Zhanjiang to carry out, the defoliator oranges and tangerines long paper moth that the control paulownia is taken, and insect mainly is in the middle instar larvae stage, and a small amount of mature larva, pupa and adult are arranged.The insect population radix on experimental investigation ground before the spray medicine, and gather 20 larvas in contrast.Weight percentage is that 15% green muscardine fungus-500 times of nimbin emulsion dilutions evenly are sprayed on leaf surface afterwards, and every mu of consumption 100ml after spray medicine 24h, 20 larvas of each treatment region random acquisition, in indoor feeding, observes the polypide death condition.
Table 2 green muscardine fungus and nimbin mixed woodland control oranges and tangerines long paper moth Toxicity Analysis
Annotate: in table, " A " represents adult, and " L " represents larva, and " P " represents pupa, and the adult that sprouts wings in process of the test all is considered as surviving, and contrast examination worm is four mixing that the district gathers.
Above-described embodiment is the better embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included in protection scope of the present invention.
Claims (1)
1. compounded pesticides is characterized in that the weight proportion of each raw material is as follows:
Nimbin 0.2g
Green muscardine fungus 9.8g (15,000,000,000 spores/g)
Emulsifier tween 10g
Stabilizing agent epoxidized soybean oil 10g
Synergist Butacide 5g
Antioxidant butyl alkyl anisole 0.5g
Cosolvent isoamyl alcohol 14.5g
Organic solvent dimethylbenzene 55g
The preparation process of above-mentioned formula insecticide is: accurately take the high cryptogam of green muscardine fungus and nimbin raw medicine, first nimbin raw medicine is dissolved in the solvent that contains Partial Antioxidation agent butyl alkyl anisole, cosolvent isoamyl alcohol and stabilizing agent epoxidized soybean oil, add again the high cryptogam of green muscardine fungus, emulsifier tween, synergist Butacide, airtight stirring 10-15min, add again residue antioxidant, stabilizing agent and organic solvent dimethylbenzene, and be adjusted to aimed concn, continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010524617 CN101984828B (en) | 2010-10-29 | 2010-10-29 | Compound pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010524617 CN101984828B (en) | 2010-10-29 | 2010-10-29 | Compound pesticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101984828A CN101984828A (en) | 2011-03-16 |
| CN101984828B true CN101984828B (en) | 2013-06-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010524617 Expired - Fee Related CN101984828B (en) | 2010-10-29 | 2010-10-29 | Compound pesticide |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535387A (en) * | 2013-10-15 | 2014-01-29 | 黄学松 | Slow-release pesticide |
| CN107950551A (en) * | 2017-12-25 | 2018-04-24 | 云南保山嘉宏农业开发（集团）有限公司 | Pure biology neem-based insecticide preparation process |
| CN107969439A (en) * | 2017-12-25 | 2018-05-01 | 云南保山嘉宏农业开发（集团）有限公司 | Pure biology neem-based insecticide formula |
| CN110269067A (en) * | 2018-03-16 | 2019-09-24 | 成都特普生物科技股份有限公司 | A kind of prevention and treatment agricultural has the combination preparation of pest type |
| CN110447661B (en) * | 2019-08-19 | 2021-08-03 | 重庆谷百奥生物研究院有限公司 | Application of metarhizium anisopliae in preparation of medicament for preventing and treating potato late blight |
| CN115104632A (en) * | 2022-08-01 | 2022-09-27 | 福建农林大学 | Compound oil suspension emulsion compounded by metarhizium anisopliae and azadirachtin as well as preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1357239A (en) * | 2001-12-27 | 2002-07-10 | 海南英得利生物技术开发有限公司 | Nim extractive and biological pesticide nim prepn |
| CN1802928A (en) * | 2005-12-26 | 2006-07-19 | 重庆大学 | Mixed insecticide of green muscardine fungus and pyrethrum group and its preparation method |
-
2010
- 2010-10-29 CN CN 201010524617 patent/CN101984828B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1357239A (en) * | 2001-12-27 | 2002-07-10 | 海南英得利生物技术开发有限公司 | Nim extractive and biological pesticide nim prepn |
| CN1802928A (en) * | 2005-12-26 | 2006-07-19 | 重庆大学 | Mixed insecticide of green muscardine fungus and pyrethrum group and its preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 杀蝗绿僵菌与植物源农药混用对亚洲小车蝗的杀虫效果;高书晶,等;《农药》;20101010;第49卷(第10期);第757-759页 * |
| 高书晶,等.杀蝗绿僵菌与植物源农药混用对亚洲小车蝗的杀虫效果.《农药》.2010,第49卷(第10期),第757-759页. |
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